Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 439,636 T→C G230G (GGA→GGG)  ispA ← geranyltranstransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913439,6360TC100.0% 750.2 / NA 214G230G (GGA→GGG) ispAgeranyltranstransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (96/118);  total (96/118)

Only 100 of 223 total aligned reads displayed.

CCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTC  >  NC_000913/439546‑439724
                                                                                          |                                                                                        
ccGGGCTTGCTCAAGACCCAGAAGTGTAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATcccc                                                                                        <  2:347695/93‑1 (MQ=255)
ccGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATcccc                                                                                        <  1:5776620/93‑1 (MQ=255)
  gggCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCcca                                                                                      <  1:5039321/93‑1 (MQ=255)
  gggCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCcca                                                                                      <  2:495068/93‑1 (MQ=255)
    gCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCTACGTTGCAGTATCCCccacc                                                                                    <  2:3672222/93‑1 (MQ=255)
    gCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGAGTTTTCCCAACGTTGCAGTATCCCccacc                                                                                    >  2:1628977/1‑93 (MQ=255)
      ttGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCcaccac                                                                                  >  1:1615376/1‑93 (MQ=255)
       tGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACcaca                                                                                 <  1:3162872/93‑1 (MQ=255)
       tGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACcaca                                                                                 <  2:1940519/93‑1 (MQ=255)
        gCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACAt                                                                                >  2:1063005/1‑93 (MQ=255)
         cTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATc                                                                               <  2:1338467/93‑1 (MQ=255)
         cTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATc                                                                               <  2:307546/93‑1 (MQ=255)
          tCAAGACGCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATACCCCACCACATcc                                                                              >  2:5231975/1‑93 (MQ=255)
          tCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATcc                                                                              <  2:3607499/93‑1 (MQ=255)
          tCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATcc                                                                              <  1:840577/93‑1 (MQ=255)
           cAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCa                                                                             <  2:1528259/93‑1 (MQ=255)
           cAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCa                                                                             <  1:1423988/93‑1 (MQ=255)
             aGACCCAGAAGTGCAGGGTAGGTACTTTTCCCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTACCCCCCACCACATCCAgg                                                                           <  1:2791271/93‑1 (MQ=255)
             aGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAgg                                                                           <  2:4867088/93‑1 (MQ=255)
             aGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAgg                                                                           >  1:2532461/1‑93 (MQ=255)
             aGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAgg                                                                           >  1:6131879/1‑93 (MQ=255)
              gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGa                                                                          >  2:2225487/1‑93 (MQ=255)
              gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGa                                                                          <  2:2967150/93‑1 (MQ=255)
              gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGa                                                                          <  1:5515105/93‑1 (MQ=255)
              gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGa                                                                          >  2:2696299/1‑93 (MQ=255)
              gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGa                                                                          >  1:2032826/1‑93 (MQ=255)
              gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGa                                                                          <  1:629675/93‑1 (MQ=255)
               aCCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGAt                                                                         <  2:5554265/93‑1 (MQ=255)
               aCCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGAt                                                                         <  2:2357475/93‑1 (MQ=255)
                 ccAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGt                                                                       <  2:1332517/93‑1 (MQ=255)
                  cAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTc                                                                      >  1:5583947/1‑93 (MQ=255)
                   aGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCa                                                                     >  1:5871837/1‑93 (MQ=255)
                    gAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCAt                                                                    <  1:1787245/93‑1 (MQ=255)
                      aGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATcc                                                                  <  2:2066585/93‑1 (MQ=255)
                      aGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATcc                                                                  >  2:1214323/1‑93 (MQ=255)
                       gTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCt                                                                 <  2:6015109/93‑1 (MQ=255)
                          cAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGaa                                                              <  1:1295437/93‑1 (MQ=255)
                              gtaggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTg                                                          <  2:393750/93‑1 (MQ=255)
                               taggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTgg                                                         <  1:4101416/93‑1 (MQ=255)
                               taggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTgg                                                         <  2:4134788/93‑1 (MQ=255)
                                 ggtaCTTTTACCAAGTTGCTGGTCGGCCCCCTGCCGTTTTCCCAGCGTTGCAGTATCCCGCACCACATCCAGGATGTCATCCTGAACCTGGaa                                                       <  2:5618238/93‑1 (MQ=255)
                                 ggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGaa                                                       >  2:3892503/1‑93 (MQ=255)
                                 ggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGaa                                                       >  2:5644719/1‑93 (MQ=255)
                                 ggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGaa                                                       <  2:505900/93‑1 (MQ=255)
                                 ggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGaa                                                       <  2:5494668/93‑1 (MQ=255)
                                   taCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGaagg                                                     <  2:3292944/93‑1 (MQ=255)
                                      ttttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggcaa                                                  >  1:5960137/1‑93 (MQ=255)
                                      ttttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggcaa                                                  >  1:5398299/1‑93 (MQ=255)
                                       tttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggcaag                                                 <  2:5466437/93‑1 (MQ=255)
                                         tACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggcaaggc                                               <  2:2504507/93‑1 (MQ=255)
                                          aCCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGcc                                              >  2:6116567/1‑93 (MQ=255)
                                           ccAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCg                                             >  2:141412/1‑93 (MQ=255)
                                            cAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGa                                            <  2:6043675/93‑1 (MQ=255)
                                            cAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGa                                            <  1:2351676/93‑1 (MQ=255)
                                             aaGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGAt                                           <  2:2571267/93‑1 (MQ=255)
                                              aGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATg                                          <  2:2152223/93‑1 (MQ=255)
                                                 tGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctc                                       <  1:943060/93‑1 (MQ=255)
                                                  gCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctct                                      <  2:3756379/93‑1 (MQ=255)
                                                   cTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctctc                                     <  1:1012763/93‑1 (MQ=255)
                                                   cTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctctc                                     >  2:4480700/1‑93 (MQ=255)
                                                   cTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctctc                                     >  2:1765714/1‑93 (MQ=255)
                                                   cTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctctc                                     >  1:3049090/1‑93 (MQ=255)
                                                   cTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctctc                                     <  2:902324/93‑1 (MQ=255)
                                                     ggTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTg                                   >  1:4494090/1‑93 (MQ=255)
                                                     ggTCGGAACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTg                                   >  2:4056610/1‑93 (MQ=255)
                                                       tCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCa                                 <  1:5264987/93‑1 (MQ=255)
                                                         ggCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATa                               <  2:1549307/93‑1 (MQ=255)
                                                         ggCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATa                               <  1:2293913/93‑1 (MQ=255)
                                                         ggCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATa                               <  2:4753453/93‑1 (MQ=255)
                                                         ggCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATa                               >  2:4549199/1‑93 (MQ=255)
                                                            aCCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACtt                            >  1:6041965/1‑93 (MQ=255)
                                                            aCCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACtt                            <  2:537086/93‑1 (MQ=255)
                                                            aCCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACtt                            >  2:5842671/1‑93 (MQ=255)
                                                             cccTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTg                           <  1:1322745/93‑1 (MQ=255)
                                                             cccTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTg                           <  1:3984784/93‑1 (MQ=255)
                                                              ccTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGt                          >  1:2863098/1‑93 (MQ=255)
                                                              ccTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGt                          >  1:3632798/1‑93 (MQ=255)
                                                              ccTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGt                          <  2:5545453/93‑1 (MQ=255)
                                                              ccTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGt                          <  1:4018177/93‑1 (MQ=255)
                                                              ccTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGt                          <  2:1108951/93‑1 (MQ=255)
                                                               cTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTc                         <  1:2934486/93‑1 (MQ=255)
                                                               cTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTc                         <  1:4613144/93‑1 (MQ=255)
                                                                   cGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGt                     <  2:2397851/93‑1 (MQ=255)
                                                                     ttttCCCACCGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                   >  2:6314345/1‑93 (MQ=255)
                                                                      tttCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAcc                  <  1:4804826/93‑1 (MQ=255)
                                                                       ttCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCg                 <  2:5983791/93‑1 (MQ=255)
                                                                          ccAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCa              >  1:1246155/1‑93 (MQ=255)
                                                                           cAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCag             >  2:6230004/1‑93 (MQ=255)
                                                                              cGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGc          <  1:3952927/93‑1 (MQ=255)
                                                                              cGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGc          <  1:2904437/93‑1 (MQ=255)
                                                                              cGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGc          <  1:2610038/93‑1 (MQ=255)
                                                                                ttGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCac        <  2:1590178/93‑1 (MQ=255)
                                                                                ttGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCac        <  1:2413660/93‑1 (MQ=255)
                                                                                  gCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacga      >  2:65525/1‑93 (MQ=255)
                                                                                   cAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacgac     <  2:428530/93‑1 (MQ=255)
                                                                                   cAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacgac     <  1:1912379/93‑1 (MQ=255)
                                                                                    aGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacgacg    >  1:344887/1‑93 (MQ=255)
                                                                                     gTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGt   >  2:255810/1‑93 (MQ=255)
                                                                                     gTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGt   >  2:2323344/1‑93 (MQ=255)
                                                                                      tATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTc  <  2:4033103/93‑1 (MQ=255)
                                                                                          |                                                                                        
CCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTC  >  NC_000913/439546‑439724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTA  >  NC_000913/439546‑439729
                                                                                          |                                                                                             
CCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCC                                                                                            <  SRR4302537.5816217/94‑1 (MQ=60)
  GGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCAC                                                                                          <  SRR4302537.5074016/94‑1 (MQ=60)
   GGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACC                                                                                         >  SRR4302537.2034835/1‑94 (MQ=60)
     CTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCAC                                                                                       >  SRR4302537.1626661/1‑94 (MQ=60)
      TTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACA                                                                                      >  SRR4302537.2341681/1‑94 (MQ=60)
       TGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACAT                                                                                     <  SRR4302537.3185078/94‑1 (MQ=60)
       TGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACAT                                                                                     <  SRR4302537.5579277/94‑1 (MQ=60)
        GCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGGTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATC                                                                                    >  SRR4302537.4583922/1‑94 (MQ=60)
         CTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCC                                                                                   <  SRR4302537.3023498/94‑1 (MQ=60)
          TCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCA                                                                                  <  SRR4302537.6471702/94‑1 (MQ=60)
          TCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCA                                                                                  <  SRR4302537.846336/94‑1 (MQ=60)
           CAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAG                                                                                 <  SRR4302537.1433904/94‑1 (MQ=60)
            AAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGG                                                                                >  SRR4302537.6174030/1‑94 (MQ=60)
            AAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGG                                                                                <  SRR4302537.775838/94‑1 (MQ=60)
            AAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGG                                                                                >  SRR4302537.2550231/1‑94 (MQ=60)
             AGACCCAGAAGTGCAGGGTAGGTACTTTTCCCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTACCCCCCACCACATCCAGGA                                                                               <  SRR4302537.2810782/94‑1 (MQ=60)
             AGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGA                                                                               >  SRR4302537.2046995/1‑94 (MQ=60)
              GACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGAT                                                                              <  SRR4302537.633957/94‑1 (MQ=60)
              GACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGAT                                                                              <  SRR4302537.5552763/94‑1 (MQ=60)
                CCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGT                                                                            <  SRR4302537.599555/94‑1 (MQ=60)
                 CCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTC                                                                           >  SRR4302537.5622120/1‑94 (MQ=60)
                  CAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCA                                                                          >  SRR4302537.5912116/1‑94 (MQ=60)
                  CAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCA                                                                          >  SRR4302537.2088068/1‑94 (MQ=60)
                   AGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCAT                                                                         <  SRR4302537.6107970/94‑1 (MQ=60)
                    GAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATC                                                                        <  SRR4302537.1799737/94‑1 (MQ=60)
                    GAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATC                                                                        <  SRR4302537.4715516/94‑1 (MQ=60)
                          CAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAAC                                                                  <  SRR4302537.1304443/94‑1 (MQ=60)
                             GGTAGGTACTTTTACCAAGTTGCTGTGCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTG                                                               <  SRR4302537.4004675/94‑1 (MQ=60)
                               TAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGA                                                             <  SRR4302537.4130053/94‑1 (MQ=60)
                                AGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAA                                                            <  SRR4302537.2435926/94‑1 (MQ=60)
                                  GTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGG                                                          >  SRR4302537.5255566/1‑94 (MQ=60)
                                  GTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGG                                                          <  SRR4302537.1846185/94‑1 (MQ=60)
                                  GTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGG                                                          >  SRR4302537.1613588/1‑94 (MQ=60)
                                     CTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAA                                                       >  SRR4302537.6001046/1‑94 (MQ=60)
                                     CTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAA                                                       >  SRR4302537.1601296/1‑94 (MQ=60)
                                     CTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAA                                                       >  SRR4302537.5435150/1‑94 (MQ=60)
                                        TTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGC                                                    <  SRR4302537.2072878/94‑1 (MQ=60)
                                         TACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCC                                                   <  SRR4302537.3452701/94‑1 (MQ=60)
                                           CCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCGCCCACCACATCCAGGATGTCATCCTGCACCTGGAAGGCCATGCCGA                                                 >  SRR4302537.6035552/1‑94 (MQ=60)
                                           CCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGA                                                 >  SRR4302537.5849468/1‑94 (MQ=60)
                                            CAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGAT                                                <  SRR4302537.2368234/94‑1 (MQ=60)
                                             AAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATG                                               >  SRR4302537.4891262/1‑94 (MQ=60)
                                                TTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTC                                            <  SRR4302537.1325328/94‑1 (MQ=60)
                                                TTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTC                                            <  SRR4302537.1412839/94‑1 (MQ=60)
                                                 TGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCT                                           <  SRR4302537.949571/94‑1 (MQ=60)
                                                  GCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTC                                          >  SRR4302537.3070338/1‑94 (MQ=60)
                                                  GCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTC                                          >  SRR4302537.4019418/1‑94 (MQ=60)
                                                   CTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCT                                         <  SRR4302537.1019744/94‑1 (MQ=60)
                                                    TGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTG                                        >  SRR4302537.2819881/1‑94 (MQ=60)
                                                    TGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTG                                        >  SRR4302537.4525179/1‑94 (MQ=60)
                                                       TCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCAT                                     <  SRR4302537.5301216/94‑1 (MQ=60)
                                                       TCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCAT                                     <  SRR4302537.2399937/94‑1 (MQ=60)
                                                         GGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATAC                                   <  SRR4302537.2310106/94‑1 (MQ=60)
                                                         GGCACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATAC                                   >  SRR4302537.5661947/1‑94 (MQ=60)
                                                          GCACCCTGGCGTTTCCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGACCCTGGAAGGCAAGGCCGATGCTCTCTGCATACT                                  <  SRR4302537.3613583/94‑1 (MQ=60)
                                                           CACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTT                                 >  SRR4302537.6083468/1‑94 (MQ=60)
                                                            CCCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTG                                <  SRR4302537.6178840/94‑1 (MQ=60)
                                                            ACCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTG                                >  SRR4302537.2929513/1‑94 (MQ=60)
                                                             CCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGT                               >  SRR4302537.3658394/1‑94 (MQ=60)
                                                             CCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGT                               >  SRR4302537.2883172/1‑94 (MQ=60)
                                                             CCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGT                               <  SRR4302537.4012684/94‑1 (MQ=60)
                                                             CCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGT                               >  SRR4302537.2627916/1‑94 (MQ=60)
                                                             CCCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGT                               <  SRR4302537.1331967/94‑1 (MQ=60)
                                                              CCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTC                              <  SRR4302537.4046308/94‑1 (MQ=60)
                                                              CCTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTC                              >  SRR4302537.4058528/1‑94 (MQ=60)
                                                               CTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCG                             >  SRR4302537.3871043/1‑94 (MQ=60)
                                                               CTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCG                             <  SRR4302537.2955090/94‑1 (MQ=60)
                                                               CTGGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCG                             <  SRR4302537.4645089/94‑1 (MQ=60)
                                                                 GGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAG                           <  SRR4302537.1301127/94‑1 (MQ=60)
                                                                 GGCGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAG                           >  SRR4302537.4350135/1‑94 (MQ=60)
                                                                   CGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTA                         >  SRR4302537.5343236/1‑94 (MQ=60)
                                                                   CGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTA                         >  SRR4302537.2978274/1‑94 (MQ=60)
                                                                   CGTTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTA                         <  SRR4302537.2810118/94‑1 (MQ=60)
                                                                     TTTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACC                       >  SRR4302537.395959/1‑94 (MQ=60)
                                                                      TTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCG                      <  SRR4302537.4837968/94‑1 (MQ=60)
                                                                      TTTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCG                      <  SRR4302537.931464/94‑1 (MQ=60)
                                                                       TTCCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGG                     <  SRR4302537.2641084/94‑1 (MQ=60)
                                                                         CCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCA                   >  SRR4302537.1254836/1‑94 (MQ=60)
                                                                         CCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCA                   >  SRR4302537.5880220/1‑94 (MQ=60)
                                                                         CCCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCA                   >  SRR4302537.4532566/1‑94 (MQ=60)
                                                                          CCAACGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAG                  >  SRR4302537.2898501/1‑94 (MQ=60)
                                                                              CGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCA              <  SRR4302537.2924842/94‑1 (MQ=60)
                                                                              CGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCA              <  SRR4302537.2628295/94‑1 (MQ=60)
                                                                              CGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCA              >  SRR4302537.3079516/1‑94 (MQ=60)
                                                                              CGTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCA              <  SRR4302537.3980579/94‑1 (MQ=60)
                                                                               GTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCAC             >  SRR4302537.5369694/1‑94 (MQ=60)
                                                                               GTTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCAC             >  SRR4302537.5454082/1‑94 (MQ=60)
                                                                                TTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACG            <  SRR4302537.2430645/94‑1 (MQ=60)
                                                                                TTGCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACG            <  SRR4302537.1706338/94‑1 (MQ=60)
                                                                                  GCAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGAC          <  SRR4302537.1158375/94‑1 (MQ=60)
                                                                                   CAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACG         <  SRR4302537.1925653/94‑1 (MQ=60)
                                                                                   CAGTATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACG         >  SRR4302537.347284/1‑94 (MQ=60)
                                                                                       ATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCT     >  SRR4302537.2419507/1‑94 (MQ=60)
                                                                                       ATCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCT     >  SRR4302537.3908284/1‑94 (MQ=60)
                                                                                        TCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTT    <  SRR4302537.1581639/94‑1 (MQ=60)
                                                                                        TCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTT    <  SRR4302537.4549860/94‑1 (MQ=60)
                                                                                        TCCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTT    <  SRR4302537.1162216/94‑1 (MQ=60)
                                                                                         CCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTT   >  SRR4302537.1939014/1‑94 (MQ=60)
                                                                                         CCCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTT   >  SRR4302537.5944336/1‑94 (MQ=60)
                                                                                          CCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTA  >  SRR4302537.446502/1‑94 (MQ=60)
                                                                                          |                                                                                             
CCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTA  >  NC_000913/439546‑439729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑