Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 451,591 T→C V393V (GTA→GTG)  ampG ← AmpG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913451,5910TC96.0% 628.2 / 11.2 199V393V (GTA→GTG) ampGAmpG
Reads supporting (aligned to +/- strand):  ref base T (4/4);  new base C (102/88);  total (106/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 211 total aligned reads displayed.

TGCCGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCGCC  >  NC_000913/451501‑451679
                                                                                          |                                                                                        
tGCCGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCAc                                                                                        <  1:3064142/93‑1 (MQ=255)
tGCCGGATATGCGGTACGGGAGATAAAGTTGACATTTACTCGTGTATATTCAAGAGTCTGGCGGCACACCAGCAGCAAAATAAGACCGGGCAc                                                                                        >  2:46724/1‑93 (MQ=255)
 gCCGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACg                                                                                       <  2:3584900/93‑1 (MQ=255)
   cgaatatGCGGTACGGGAGATAAAGTTGTCATTTACTCTTGTATATTCAAGCGTCTGGCGGCAATCCAGCAGCAAAATAAGCCCTGGCACGGc                                                                                     <  2:1018630/90‑1 (MQ=255)
   cGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGc                                                                                     >  2:3478177/1‑93 (MQ=255)
   cGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGc                                                                                     <  1:3659652/93‑1 (MQ=255)
    ggATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCa                                                                                    >  2:2901750/1‑93 (MQ=255)
     gATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAg                                                                                   >  1:6103958/1‑93 (MQ=255)
      atatGCGGTACTGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAgc                                                                                  <  2:173627/93‑1 (MQ=255)
      atatGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAgc                                                                                  >  1:3622813/1‑93 (MQ=255)
      atatGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAgc                                                                                  >  2:3706153/1‑93 (MQ=255)
        atGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAgcgg                                                                                >  2:2996223/1‑93 (MQ=255)
         tGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAgcggc                                                                               >  2:4008716/1‑93 (MQ=255)
          gCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAgcggcg                                                                              >  1:5803134/1‑93 (MQ=255)
           cGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGa                                                                             <  1:149326/93‑1 (MQ=255)
           cGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGa                                                                             <  2:4465111/93‑1 (MQ=255)
            ggTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGAc                                                                            >  1:5208623/1‑93 (MQ=255)
            ggTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGAc                                                                            >  2:3247813/1‑93 (MQ=255)
            ggTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGAc                                                                            <  2:5060818/93‑1 (MQ=255)
            ggTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGAc                                                                            <  2:829863/93‑1 (MQ=255)
             gTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACg                                                                           <  1:4664381/93‑1 (MQ=255)
              tACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACgg                                                                          <  1:1052662/93‑1 (MQ=255)
              gaCGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACgg                                                                          <  1:1180445/92‑1 (MQ=255)
               aCGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGga                                                                         <  2:1840519/93‑1 (MQ=255)
                cGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGgag                                                                        >  2:702869/1‑93 (MQ=255)
                cGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGgag                                                                        <  1:558717/93‑1 (MQ=255)
                 gggAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGgaga                                                                       <  1:537266/93‑1 (MQ=255)
                  ggAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGaa                                                                      <  2:1455347/93‑1 (MQ=255)
                  ggAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGaa                                                                      >  1:4215220/1‑93 (MQ=255)
                   gagaTAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAat                                                                     >  1:6360082/1‑93 (MQ=255)
                   gagaTAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAat                                                                     >  1:3480131/1‑93 (MQ=255)
                   gagaTAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAat                                                                     <  2:5313189/93‑1 (MQ=255)
                    agaTAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAata                                                                    <  2:2136373/93‑1 (MQ=255)
                       tAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAatagat                                                                 >  1:1634328/1‑93 (MQ=255)
                       tAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAatagat                                                                 <  1:5857506/93‑1 (MQ=255)
                       tAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAatagat                                                                 <  1:4903847/93‑1 (MQ=255)
                        aaaGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAatagata                                                                <  1:2279304/93‑1 (MQ=255)
                         aaGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAatagatag                                                               <  1:6310059/93‑1 (MQ=255)
                         aaGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAatagatag                                                               >  1:2420125/1‑93 (MQ=255)
                          aGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAAtagataga                                                              <  1:839881/93‑1 (MQ=255)
                          aGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAAtagataga                                                              >  2:3524481/1‑93 (MQ=255)
                            ttGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAAt                                                            >  1:2110251/1‑93 (MQ=255)
                            ttGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAAt                                                            <  1:819808/93‑1 (MQ=255)
                            ttGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAAt                                                            <  2:3422622/93‑1 (MQ=255)
                             tGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATg                                                           >  2:1399039/1‑93 (MQ=255)
                              gTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGt                                                          >  1:3214475/1‑93 (MQ=255)
                                 aTTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGa                                                       >  2:2643274/1‑93 (MQ=255)
                                   ttACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGGCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGAcc                                                     >  1:2214743/1‑93 (MQ=255)
                                    tACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCa                                                    >  1:5423846/1‑93 (MQ=255)
                                    tACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCa                                                    >  2:3815661/1‑93 (MQ=255)
                                      cTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGc                                                  <  1:2008586/93‑1 (MQ=255)
                                      cTCGTGTATATTCAAGCGTCTCGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGc                                                  >  1:1924728/1‑93 (MQ=255)
                                       tCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGcc                                                 <  1:6447905/93‑1 (MQ=255)
                                         gtgtATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCgt                                               >  1:4096028/1‑93 (MQ=255)
                                         gtgtATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCgt                                               <  2:1547579/93‑1 (MQ=255)
                                         gtgtATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCgg                                               >  2:599867/1‑92 (MQ=255)
                                           gtATATTCAAGCGTCTGGCGGGAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGGCGGAGAATAGATAGAATGTCGACCAGCCgtgc                                             >  2:6151881/1‑92 (MQ=255)
                                             atatTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGc                                           <  1:246920/93‑1 (MQ=255)
                                               atTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCtt                                         <  2:1115999/93‑1 (MQ=255)
                                               atTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCtt                                         >  1:3181589/1‑93 (MQ=255)
                                                ttCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTc                                        >  2:1794330/1‑93 (MQ=255)
                                                ttCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTc                                        >  1:4049393/1‑93 (MQ=255)
                                                 tCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTca                                       >  1:4459728/1‑93 (MQ=255)
                                                  cAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTcaa                                      <  1:1759050/93‑1 (MQ=255)
                                                     gCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTcaacaa                                   >  1:5735032/1‑93 (MQ=255)
                                                      cGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACaaa                                  >  2:1952614/1‑93 (MQ=255)
                                                       gTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAaac                                 >  1:4803825/1‑93 (MQ=255)
                                                        tCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAaacc                                >  1:4568669/1‑93 (MQ=255)
                                                         cTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAaacca                               <  1:1699355/93‑1 (MQ=255)
                                                         cTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAaacca                               <  1:5348879/93‑1 (MQ=255)
                                                          tGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAaaccaa                              <  1:2030522/93‑1 (MQ=255)
                                                            gcggcAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAaaccaacc                            <  2:1375699/93‑1 (MQ=255)
                                                              ggcAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCg                          >  1:5681566/1‑93 (MQ=255)
                                                                  aaCCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCAc                      >  1:367389/1‑93 (MQ=255)
                                                                  aaCCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCAc                      >  1:417513/1‑93 (MQ=255)
                                                                   aCCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACg                     >  2:54671/1‑93 (MQ=255)
                                                                   aCCAGCAGCAAAATAACCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACg                     <  2:2815524/93‑1 (MQ=255)
                                                                    ccAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGGCGGGGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAAACAAACCCCCCCgg                    >  2:2049973/1‑93 (MQ=255)
                                                                    ccAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACgg                    >  1:2437358/1‑93 (MQ=255)
                                                                     cagcagCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGCCCAGCCGTGTGCTTCAACAAACCAACCCGCCACggg                   <  2:2433974/93‑1 (MQ=255)
                                                                       gcagcaAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGc                 >  2:3962170/1‑93 (MQ=255)
                                                                       gcagcaAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGc                 <  1:4684912/93‑1 (MQ=255)
                                                                       gcagcaAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGATTCAACAAACCAACCCGCCACGGGGc                 >  2:5218901/1‑93 (MQ=255)
                                                                          gcaAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGa              >  2:2673429/1‑93 (MQ=255)
                                                                          gcaAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGa              >  1:496510/1‑93 (MQ=255)
                                                                          gcaAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGa              <  2:2917843/93‑1 (MQ=255)
                                                                          gcaAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGa              >  1:2855857/1‑93 (MQ=255)
                                                                            aaaaTAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACa            <  2:6438278/93‑1 (MQ=255)
                                                                             aaaTAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACAt           >  2:6121945/1‑93 (MQ=255)
                                                                              aaTAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATa          >  1:3017559/1‑93 (MQ=255)
                                                                                tAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATaaa        >  1:5007299/1‑93 (MQ=255)
                                                                                tAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATaaa        <  2:6222588/93‑1 (MQ=255)
                                                                                  aGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACt      >  2:1227080/1‑93 (MQ=255)
                                                                                  aGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACt      <  2:6293507/93‑1 (MQ=255)
                                                                                   gCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGCCCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTc     <  1:4852003/93‑1 (MQ=255)
                                                                                   gCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTTTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTc     <  2:2345008/93‑1 (MQ=255)
                                                                                   gCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTc     <  1:5073248/93‑1 (MQ=255)
                                                                                      cTGGCACGGCAGCGGCGAGGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAACCCACCCCGCCACGGGGCCGACATAAACTCGcc  <  2:6385348/93‑1 (MQ=255)
                                                                                      cTGGCACGGCAGCGGCGACGGAGAATAGATAGGATGTCGACCAGCCGGGTGCTTCAACAAACCCACCCGCCACGGGGCTGACATTAACTCGcc  >  2:4299515/1‑93 (MQ=255)
                                                                                      cTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATACACTCGcc  >  2:4618214/1‑93 (MQ=255)
                                                                                          |                                                                                        
TGCCGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCGCC  >  NC_000913/451501‑451679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCTGCCGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCGCCCTACA  >  NC_000913/451498‑451684
                                                                                             |                                                                                             
ACCTGCCGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGC                                                                                               <  SRR4302537.4561570/94‑1 (MQ=60)
ACCTGCCGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGC                                                                                               <  SRR4302537.2634634/94‑1 (MQ=60)
ACCTGCCGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGC                                                                                               <  SRR4302537.2464325/94‑1 (MQ=60)
   TGCCGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACG                                                                                            <  SRR4302537.3085489/94‑1 (MQ=60)
      cggattatcggtacgggaagaaaaggtggtatttacttgtttattatccAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCA                                                                                         <  SRR4302537.2656974/45‑1 (MQ=60)
      CGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCA                                                                                         <  SRR4302537.3685408/94‑1 (MQ=60)
      CGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCA                                                                                         >  SRR4302537.4914030/1‑94 (MQ=60)
       GGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAG                                                                                        >  SRR4302537.1080505/1‑94 (MQ=60)
       GGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAG                                                                                        >  SRR4302537.6145896/1‑94 (MQ=60)
       GGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAG                                                                                        >  SRR4302537.3236435/1‑94 (MQ=60)
        GATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGC                                                                                       >  SRR4302537.3648347/1‑94 (MQ=60)
          TATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGG                                                                                     <  SRR4302537.4259166/94‑1 (MQ=60)
           ATGCGGTACGGGAGATAAAGTTGTCATTTTCTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGC                                                                                    <  SRR4302537.4227447/94‑1 (MQ=60)
           ATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGC                                                                                    >  SRR4302537.4961685/1‑94 (MQ=60)
            TGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCG                                                                                   >  SRR4302537.244196/1‑94 (MQ=60)
            TGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCG                                                                                   >  SRR4302537.580503/1‑94 (MQ=60)
            TGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCG                                                                                   >  SRR4302537.5842928/1‑94 (MQ=60)
              CGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGAC                                                                                 <  SRR4302537.150395/94‑1 (MQ=60)
              CGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGAC                                                                                 >  SRR4302537.5244441/1‑94 (MQ=60)
                GTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGG                                                                               <  SRR4302537.4696618/94‑1 (MQ=60)
                 TACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGA                                                                              <  SRR4302537.1059923/94‑1 (MQ=60)
                 TACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGA                                                                              <  SRR4302537.843180/94‑1 (MQ=60)
                 GACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGA                                                                              <  SRR4302537.1188658/94‑1 (MQ=60)
                  ACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAG                                                                             <  SRR4302537.1285720/94‑1 (MQ=60)
                  ACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAG                                                                             <  SRR4302537.1674465/94‑1 (MQ=60)
                   CGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGA                                                                            <  SRR4302537.562562/94‑1 (MQ=60)
                    GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAA                                                                           <  SRR4302537.740814/94‑1 (MQ=60)
                    GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAA                                                                           <  SRR4302537.540953/94‑1 (MQ=60)
                    GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAA                                                                           >  SRR4302537.4244607/1‑94 (MQ=60)
                     GGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAAT                                                                          >  SRR4302537.3504752/1‑94 (MQ=60)
                     GGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAAT                                                                          >  SRR4302537.6403475/1‑94 (MQ=60)
                      GAGATAAAGTTGTCGTTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATA                                                                         >  SRR4302537.1583993/1‑94 (MQ=60)
                       AGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAG                                                                        <  SRR4302537.2047512/94‑1 (MQ=60)
                        GATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGA                                                                       <  SRR4302537.5524550/94‑1 (MQ=60)
                         ATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGAT                                                                      >  SRR4302537.1645777/1‑94 (MQ=60)
                         ATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGAT                                                                      >  SRR4302537.5875075/1‑94 (MQ=60)
                          TAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATA                                                                     <  SRR4302537.4937695/94‑1 (MQ=60)
                          TAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATA                                                                     <  SRR4302537.4563826/94‑1 (MQ=60)
                          TAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATA                                                                     <  SRR4302537.5897684/94‑1 (MQ=60)
                           AAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAG                                                                    <  SRR4302537.2295394/94‑1 (MQ=60)
                           AAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAG                                                                    >  SRR4302537.5860357/1‑94 (MQ=60)
                           AAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAG                                                                    >  SRR4302537.2437179/1‑94 (MQ=60)
                           AAAATTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAG                                                                    <  SRR4302537.5438431/94‑1 (MQ=60)
                            AAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGA                                                                   <  SRR4302537.6353261/94‑1 (MQ=60)
                            AAGTTGTCATGTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCATCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGA                                                                   <  SRR4302537.6346992/94‑1 (MQ=60)
                             AGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAA                                                                  <  SRR4302537.2043633/94‑1 (MQ=60)
                             AGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAA                                                                  <  SRR4302537.845635/94‑1 (MQ=60)
                              GTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAAT                                                                 <  SRR4302537.4860630/94‑1 (MQ=60)
                              GTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAAT                                                                 >  SRR4302537.2125125/1‑94 (MQ=60)
                               TTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATG                                                                <  SRR4302537.825438/94‑1 (MQ=60)
                               TTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATG                                                                >  SRR4302537.572618/1‑94 (MQ=60)
                               TTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATG                                                                >  SRR4302537.5394839/1‑94 (MQ=60)
                                TGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGT                                                               >  SRR4302537.3237097/1‑94 (MQ=60)
                                 TTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGGACGGCAGCGGCGACGGAGAATAGATAGAATGTC                                                              <  SRR4302537.2403765/94‑1 (MQ=60)
                                    ATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGAC                                                           >  SRR4302537.5083560/1‑94 (MQ=60)
                                    ATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGAC                                                           <  SRR4302537.3270767/94‑1 (MQ=60)
                                     TTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGGCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACC                                                          >  SRR4302537.2230363/1‑94 (MQ=60)
                                      TTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCA                                                         >  SRR4302537.5460815/1‑94 (MQ=60)
                                      TTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCA                                                         >  SRR4302537.368634/1‑94 (MQ=60)
                                       TACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAG                                                        <  SRR4302537.3211945/94‑1 (MQ=60)
                                        ACTCGTGTATATTCAAGCGTCTCGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGC                                                       >  SRR4302537.1938088/1‑94 (MQ=60)
                                         CTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCC                                                      <  SRR4302537.2022585/94‑1 (MQ=60)
                                         CTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCC                                                      <  SRR4302537.2256418/94‑1 (MQ=60)
                                         CTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCC                                                      <  SRR4302537.3110200/94‑1 (MQ=60)
                                          TCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCG                                                     <  SRR4302537.6491641/94‑1 (MQ=60)
                                           CGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGT                                                    >  SRR4302537.4124630/1‑94 (MQ=60)
                                             TGTATATTCAAGCGTCTGGCGCCAACCCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGT                                                  <  SRR4302537.2481178/94‑1 (MQ=60)
                                              GTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTG                                                 <  SRR4302537.2134763/94‑1 (MQ=60)
                                              GTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTG                                                 <  SRR4302537.3539670/94‑1 (MQ=60)
                                                ATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCT                                               >  SRR4302537.3718530/1‑94 (MQ=60)
                                                ATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCC                                               <  SRR4302537.248673/94‑1 (MQ=60)
                                                 TATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTT                                              >  SRR4302537.3203950/1‑94 (MQ=60)
                                                 TATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTT                                              >  SRR4302537.1762486/1‑94 (MQ=60)
                                                  ATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTC                                             >  SRR4302537.5929409/1‑94 (MQ=60)
                                                  ATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTC                                             >  SRR4302537.4077706/1‑94 (MQ=60)
                                                  ATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTC                                             >  SRR4302537.2142391/1‑94 (MQ=60)
                                                   TTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCA                                            >  SRR4302537.4490563/1‑94 (MQ=60)
                                                     CAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAAC                                          <  SRR4302537.1771311/94‑1 (MQ=60)
                                                      AAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACA                                         >  SRR4302537.6385694/1‑94 (MQ=60)
                                                       AGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAA                                        >  SRR4302537.5774296/1‑94 (MQ=60)
                                                         CGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAAC                                      >  SRR4302537.4836964/1‑94 (MQ=60)
                                                         CGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAAC                                      <  SRR4302537.2009592/94‑1 (MQ=60)
                                                          GTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACC                                     >  SRR4302537.4600287/1‑94 (MQ=60)
                                                           TCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCA                                    <  SRR4302537.480251/94‑1 (MQ=60)
                                                            CTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAA                                   <  SRR4302537.1711237/94‑1 (MQ=60)
                                                            CTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAA                                   <  SRR4302537.5385526/94‑1 (MQ=60)
                                                             TGGCGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAAC                                  <  SRR4302537.2044676/94‑1 (MQ=60)
                                                                CGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCG                               >  SRR4302537.3040429/1‑94 (MQ=60)
                                                                CGGCAAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCG                               >  SRR4302537.5720428/1‑94 (MQ=60)
                                                                    AAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCAC                           >  SRR4302537.420411/1‑94 (MQ=60)
                                                                    AAACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCAC                           >  SRR4302537.369938/1‑94 (MQ=60)
                                                                     AACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACG                          >  SRR4302537.4677140/1‑94 (MQ=60)
                                                                     AACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCAACCAGCCGTGTGCTTCAACAAACCAACCCGCCACG                          <  SRR4302537.5191647/94‑1 (MQ=60)
                                                                      ACCAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGG                         >  SRR4302537.2454528/1‑94 (MQ=60)
                                                                        CAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGG                       <  SRR4302537.5090973/94‑1 (MQ=60)
                                                                        CAGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGG                       <  SRR4302537.500812/94‑1 (MQ=60)
                                                                         AGCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAAAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGC                      <  SRR4302537.1757377/94‑1 (MQ=60)
                                                                          GCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCC                     <  SRR4302537.893028/94‑1 (MQ=60)
                                                                          GCAGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCC                     <  SRR4302537.4717318/94‑1 (MQ=60)
                                                                            AGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGA                   >  SRR4302537.499926/1‑94 (MQ=60)
                                                                            AGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGA                   >  SRR4302537.3648631/1‑94 (MQ=60)
                                                                            AGCAAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGA                   >  SRR4302537.2875877/1‑94 (MQ=60)
                                                                               AAAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACAT                <  SRR4302537.4166179/94‑1 (MQ=60)
                                                                                AAATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATA               >  SRR4302537.3038593/1‑94 (MQ=60)
                                                                                  ATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAA             >  SRR4302537.5668921/1‑94 (MQ=60)
                                                                                  ATAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAA             >  SRR4302537.5041788/1‑94 (MQ=60)
                                                                                   AAAGCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAAC            <  SRR4302537.2898206/94‑1 (MQ=60)
                                                                                      GCCCTGGCACGGCAGCGGCGTCGGAGAATAAATAGAATTTCGGCCAGCCGTGTCCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCG         <  SRR4302537.3060014/94‑1 (MQ=60)
                                                                                      GCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGCCCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCG         <  SRR4302537.4885475/94‑1 (MQ=60)
                                                                                      GCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCACCCCGCCACGGGGCCGACATAAACTCG         <  SRR4302537.4710869/94‑1 (MQ=60)
                                                                                      GCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCG         <  SRR4302537.5108175/94‑1 (MQ=60)
                                                                                      GCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCG         <  SRR4302537.2820116/94‑1 (MQ=60)
                                                                                      GCCCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCC         >  SRR4302537.6443900/1‑94 (MQ=60)
                                                                                        CCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCGCC       >  SRR4302537.5250907/1‑94 (MQ=60)
                                                                                        CCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCGCC       >  SRR4302537.4109249/1‑94 (MQ=60)
                                                                                        CCTGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGCGCCGACATAAACCCGCC       >  SRR4302537.1916634/1‑94 (MQ=60)
                                                                                          TGGCACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGCGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCGCCCT     >  SRR4302537.2160386/1‑94 (MQ=60)
                                                                                             CACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCGCCCTACA  >  SRR4302537.3111275/1‑94 (MQ=60)
                                                                                             CACGGCAGCGCCGACGGAAAATAGATAGAATGTCGACCATCCGTGTGCTTAAACAAACCAACCCGCCACGGGGCCGACATAAACTCGCCCTACA  <  SRR4302537.2021182/94‑1 (MQ=60)
                                                                                             |                                                                                             
ACCTGCCGGATATGCGGTACGGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAACAAACCAACCCGCCACGGGGCCGACATAAACTCGCCCTACA  >  NC_000913/451498‑451684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑