Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 502,653 C→T intergenic (‑191/+47) ybaL ← / ← fsr putative transport protein/fosmidomycin resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913502,6530CT99.2% 895.1 / NA 258intergenic (‑191/+47)ybaL/fsrputative transport protein/fosmidomycin resistance protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (138/117);  minor base G (2/0);  total (140/118)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

ATTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGCGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATC  >  NC_000913/502563‑502742
                                                                                          |                                                                                         
attattGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGa                                                                                         >  1:6369921/1‑93 (MQ=255)
 ttattGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGAc                                                                                        <  1:3371939/93‑1 (MQ=255)
   attGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTg                                                                                      >  2:3439066/1‑93 (MQ=255)
   attGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTg                                                                                      <  1:2786208/93‑1 (MQ=255)
   attGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTg                                                                                      >  1:2330659/1‑93 (MQ=255)
         ttttGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAAc                                                                                <  2:870546/93‑1 (MQ=255)
         ttttGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAAc                                                                                >  2:2174127/1‑93 (MQ=255)
           ttGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGa                                                                              >  2:2370882/1‑93 (MQ=255)
              cAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGc                                                                           >  1:4623778/1‑93 (MQ=255)
              cAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGc                                                                           <  1:4058116/93‑1 (MQ=255)
              cAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGc                                                                           <  2:3324862/93‑1 (MQ=255)
              cAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGc                                                                           >  2:333684/1‑93 (MQ=255)
               aGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCa                                                                          <  2:3740201/93‑1 (MQ=255)
               aGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCCGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCa                                                                          <  1:3458139/93‑1 (MQ=255)
                gAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAg                                                                         >  2:5925455/1‑93 (MQ=255)
                  aaaaTGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGcc                                                                       <  1:5311511/93‑1 (MQ=255)
                   aaaTGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCa                                                                      >  2:4700784/1‑93 (MQ=255)
                   aaaTGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCa                                                                      >  1:4195706/1‑93 (MQ=255)
                   aaaTGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCa                                                                      >  2:1948024/1‑93 (MQ=255)
                    aaTGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCaa                                                                     <  2:773019/93‑1 (MQ=255)
                    aaTGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCaa                                                                     >  1:4507786/1‑93 (MQ=255)
                      tGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAg                                                                   >  2:690071/1‑93 (MQ=255)
                      tGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAg                                                                   >  2:256676/1‑93 (MQ=255)
                        gAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGtt                                                                 <  1:1654578/93‑1 (MQ=255)
                        gAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGtt                                                                 >  2:6438688/1‑93 (MQ=255)
                        gAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACGGTTAAACGACGCCGACAAAgct                                                                 >  1:2083639/1‑91 (MQ=255)
                         aaTTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGttt                                                                >  1:5404988/1‑93 (MQ=255)
                          aTTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTg                                                               >  2:4978594/1‑93 (MQ=255)
                          aTTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTg                                                               <  1:1206487/93‑1 (MQ=255)
                          aTTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTg                                                               <  2:5612677/93‑1 (MQ=255)
                           ttAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGc                                                              >  2:2240571/1‑93 (MQ=255)
                            tAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGcc                                                             <  2:4991540/93‑1 (MQ=255)
                               cTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCttt                                                          <  1:6240762/93‑1 (MQ=255)
                                tGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTg                                                         <  2:2488640/93‑1 (MQ=255)
                                tGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTg                                                         >  1:2277606/1‑93 (MQ=255)
                                  cAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGc                                                       <  1:4998337/93‑1 (MQ=255)
                                  cAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGc                                                       >  1:3116117/1‑93 (MQ=255)
                                   aaaaaaaGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCt                                                      >  1:4360765/1‑93 (MQ=255)
                                    aaaaaaGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTg                                                     <  2:4146901/93‑1 (MQ=255)
                                    aaaaaaGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTg                                                     >  2:1129825/1‑93 (MQ=255)
                                     aaaaaGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGc                                                    >  1:1290054/1‑93 (MQ=255)
                                      aaaaGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTTGCTGCa                                                   >  2:5531583/1‑93 (MQ=255)
                                      aaaaGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCa                                                   >  2:4492735/1‑93 (MQ=255)
                                        aaGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAAt                                                 <  1:1953983/93‑1 (MQ=255)
                                            aCGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGaaa                                             >  1:1788018/1‑93 (MQ=255)
                                            aCGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGaaa                                             >  2:4342291/1‑93 (MQ=255)
                                             cGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAAt                                            <  1:5118757/93‑1 (MQ=255)
                                             cGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAAt                                            >  2:1365760/1‑93 (MQ=255)
                                             cGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAAt                                            <  2:203619/93‑1 (MQ=255)
                                              ggATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATc                                           >  1:5183579/1‑93 (MQ=255)
                                              ggATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATc                                           <  1:4713970/93‑1 (MQ=255)
                                              ggATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATc                                           >  2:2769672/1‑93 (MQ=255)
                                                    gAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCtt                                     <  1:2973578/93‑1 (MQ=255)
                                                      aaaCAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTa                                   <  2:2124929/93‑1 (MQ=255)
                                                      aaaCAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTa                                   <  1:2459412/93‑1 (MQ=255)
                                                      aaaCAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTa                                   <  2:4785123/93‑1 (MQ=255)
                                                       aaCAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTAt                                  <  2:1812596/93‑1 (MQ=255)
                                                       aaCAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTAt                                  <  2:483648/93‑1 (MQ=255)
                                                        aCAGAAGTCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATg                                 <  1:6055846/93‑1 (MQ=255)
                                                          aGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGcc                               >  2:230475/1‑93 (MQ=255)
                                                          aGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGcc                               <  2:1889913/93‑1 (MQ=255)
                                                           gAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCg                              >  1:3841410/1‑93 (MQ=255)
                                                           gAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCg                              <  1:48802/93‑1 (MQ=255)
                                                            aaGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGGTGCAGCCAAAGTTTGCCTTTGGCTGCAAAGAAATCAGTCTTTATGCCgg                             <  2:3768242/93‑1 (MQ=255)
                                                             aGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGt                            >  1:4676486/1‑93 (MQ=255)
                                                              ggCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGtt                           >  2:2791564/1‑93 (MQ=255)
                                                               gCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTa                          <  1:1334707/93‑1 (MQ=255)
                                                                 ggTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATc                        <  2:1272806/93‑1 (MQ=255)
                                                                 ggTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATc                        <  1:591182/93‑1 (MQ=255)
                                                                  gTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATca                       <  2:5299341/93‑1 (MQ=255)
                                                                  gTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATca                       <  1:577266/93‑1 (MQ=255)
                                                                  gTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATca                       <  2:3429680/93‑1 (MQ=255)
                                                                  gTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGACGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATca                       >  2:3118905/1‑93 (MQ=255)
                                                                   tttAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATcag                      >  1:1006189/1‑93 (MQ=255)
                                                                    ttAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATcagg                     >  1:3533730/1‑93 (MQ=255)
                                                                    ttAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATcagg                     <  2:3546938/93‑1 (MQ=255)
                                                                    ttAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATcagg                     <  1:6299867/93‑1 (MQ=255)
                                                                     tAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATcaggc                    >  1:4925396/1‑93 (MQ=255)
                                                                      aaCAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATcaggca                   >  2:5269787/1‑93 (MQ=255)
                                                                      aaCAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATcaggca                   <  2:4454332/93‑1 (MQ=255)
                                                                      aaCAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATcaggca                   >  1:3967966/1‑93 (MQ=255)
                                                                       aCAGAGGAAGGGCGTATGGTGACTGTATCACGATGGAGCGAAAGTTTGGCTTTTGCTGCAATGAAATCAGTATTTGTGCTGGTTGTcggggag                  >  2:2266112/1‑93 (MQ=255)
                                                                       aCAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATcaggcag                  >  2:5126036/1‑93 (MQ=255)
                                                                        cAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATcaggcagg                 >  1:4906706/1‑93 (MQ=255)
                                                                         agagGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGa                <  1:5547029/93‑1 (MQ=255)
                                                                         agagGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGGTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGa                >  1:1869392/1‑93 (MQ=255)
                                                                          gagGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGaa               <  2:2689553/93‑1 (MQ=255)
                                                                          gagGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGaa               <  2:5020995/93‑1 (MQ=255)
                                                                           agGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAat              >  2:620274/1‑93 (MQ=255)
                                                                           agGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAat              <  2:4252656/93‑1 (MQ=255)
                                                                            ggAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAata             >  1:3575997/1‑93 (MQ=255)
                                                                            ggAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAata             <  1:6446420/93‑1 (MQ=255)
                                                                              aaaGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATg           >  2:1188534/1‑93 (MQ=255)
                                                                                aGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGt         >  1:5515075/1‑93 (MQ=255)
                                                                                   cTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCaa      >  2:2696884/1‑93 (MQ=255)
                                                                                   cTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCaa      >  1:790929/1‑93 (MQ=255)
                                                                                    ttATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAAc     >  1:562830/1‑93 (MQ=255)
                                                                                    ttATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGGCaaa     >  1:4153616/1‑92 (MQ=255)
                                                                                     tATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACa    <  2:4721214/93‑1 (MQ=255)
                                                                                     tATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACa    <  2:1669893/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGCGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATC  >  NC_000913/502563‑502742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAATTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGCGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATCCCCA  >  NC_000913/502560‑502746
                                                                                             |                                                                                             
ATAATTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGGAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGT                                                                                               >  SRR4302537.5049/1‑94 (MQ=60)
ATAATTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGT                                                                                               <  SRR4302537.1102029/94‑1 (MQ=60)
ATAATTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGT                                                                                               <  SRR4302537.606663/94‑1 (MQ=60)
  AATTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGA                                                                                             >  SRR4302537.2478729/1‑94 (MQ=60)
  AATTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGA                                                                                             >  SRR4302537.6413364/1‑94 (MQ=60)
  AATTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGA                                                                                             <  SRR4302537.798316/94‑1 (MQ=60)
    TTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGNTNGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCNTATGGTGACT                                                                                           <  SRR4302537.5594000/94‑1 (MQ=60)
    TTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACT                                                                                           <  SRR4302537.4133137/94‑1 (MQ=60)
    TTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACT                                                                                           <  SRR4302537.3395834/94‑1 (MQ=60)
    TTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACT                                                                                           >  SRR4302537.4808925/1‑94 (MQ=60)
     TATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTG                                                                                          >  SRR4302537.2347092/1‑94 (MQ=60)
      ATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGT                                                                                         <  SRR4302537.3455403/94‑1 (MQ=60)
      ATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGT                                                                                         <  SRR4302537.2805689/94‑1 (MQ=60)
       TTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTA                                                                                        <  SRR4302537.5010127/94‑1 (MQ=60)
          AATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAA                                                                                     >  SRR4302537.4413364/1‑94 (MQ=60)
          AATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAA                                                                                     <  SRR4302537.3360519/94‑1 (MQ=60)
           ATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAAC                                                                                    <  SRR4302537.5180076/94‑1 (MQ=60)
                GCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGC                                                                               >  SRR4302537.2116751/1‑94 (MQ=60)
                GCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGC                                                                               <  SRR4302537.2039815/94‑1 (MQ=60)
                GCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGC                                                                               >  SRR4302537.4655780/1‑94 (MQ=60)
                 CAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCA                                                                              <  SRR4302537.4086473/94‑1 (MQ=60)
                 CAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCA                                                                              >  SRR4302537.1037688/1‑94 (MQ=60)
                  AGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAG                                                                             >  SRR4302537.6493761/1‑94 (MQ=60)
                  AGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAG                                                                             <  SRR4302537.4680960/94‑1 (MQ=60)
                  AGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAG                                                                             <  SRR4302537.1773108/94‑1 (MQ=60)
                  AGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCCGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAG                                                                             <  SRR4302537.3482589/94‑1 (MQ=60)
                  AGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGACGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAG                                                                             >  SRR4302537.5696804/1‑94 (MQ=60)
                   GAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGC                                                                            <  SRR4302537.6295787/94‑1 (MQ=60)
                     AAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCA                                                                          >  SRR4302537.4225010/1‑94 (MQ=60)
                     AAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCA                                                                          <  SRR4302537.5347995/94‑1 (MQ=60)
                     AAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCCTATGGGGACTGTAAAACGATGCAGCCA                                                                          >  SRR4302537.5966857/1‑94 (MQ=60)
                      AAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAA                                                                         >  SRR4302537.4538958/1‑94 (MQ=60)
                        ATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAG                                                                       <  SRR4302537.5367029/94‑1 (MQ=60)
                         TGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGT                                                                      >  SRR4302537.4754319/1‑94 (MQ=60)
                          GGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTT                                                                     >  SRR4302537.4387636/1‑94 (MQ=60)
                          GGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTT                                                                     >  SRR4302537.2214287/1‑94 (MQ=60)
                           GAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTT                                                                    <  SRR4302537.1666175/94‑1 (MQ=60)
                           GAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTT                                                                    >  SRR4302537.311919/1‑94 (MQ=60)
                           GAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTT                                                                    >  SRR4302537.1891449/1‑94 (MQ=60)
                           GAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTT                                                                    >  SRR4302537.5441862/1‑94 (MQ=60)
                            AATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTG                                                                   >  SRR4302537.5344978/1‑94 (MQ=60)
                            AATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTG                                                                   >  SRR4302537.3993441/1‑94 (MQ=60)
                             ATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGC                                                                  <  SRR4302537.1214884/94‑1 (MQ=60)
                                AGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTT                                                               >  SRR4302537.5190015/1‑94 (MQ=60)
                                AGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTT                                                               >  SRR4302537.5236144/1‑94 (MQ=60)
                                 GCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTT                                                              >  SRR4302537.469473/1‑94 (MQ=60)
                                  CTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTG                                                             <  SRR4302537.6283664/94‑1 (MQ=60)
                                  CTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTG                                                             <  SRR4302537.5466506/94‑1 (MQ=60)
                                  CTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTG                                                             >  SRR4302537.2293685/1‑94 (MQ=60)
                                   TGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGG                                                            >  SRR4302537.3974073/1‑94 (MQ=60)
                                    GCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGC                                                           >  SRR4302537.3137880/1‑94 (MQ=60)
                                     CAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCT                                                          >  SRR4302537.1864785/1‑94 (MQ=60)
                                     CAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCT                                                          >  SRR4302537.4390939/1‑94 (MQ=60)
                                     CAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCT                                                          >  SRR4302537.3147304/1‑94 (MQ=60)
                                     CAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCT                                                          <  SRR4302537.5032779/94‑1 (MQ=60)
                                      AAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTG                                                         <  SRR4302537.3562564/94‑1 (MQ=60)
                                       AAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGC                                                        >  SRR4302537.1299026/1‑94 (MQ=60)
                                        AAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCA                                                       >  SRR4302537.4436487/1‑94 (MQ=60)
                                         AAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAA                                                      <  SRR4302537.2387592/94‑1 (MQ=60)
                                          AAACCGCGGATCGGAAAACAGAAGGCGGCTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAAT                                                     <  SRR4302537.3191451/94‑1 (MQ=60)
                                           AAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATG                                                    <  SRR4302537.1967574/94‑1 (MQ=60)
                                           AAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATG                                                    >  SRR4302537.4595269/1‑94 (MQ=60)
                                              CACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAA                                                 >  SRR4302537.1800512/1‑94 (MQ=60)
                                               ACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAAT                                                >  SRR4302537.5139002/1‑94 (MQ=60)
                                               ACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAAT                                                >  SRR4302537.3683971/1‑94 (MQ=60)
                                                CGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATC                                               >  SRR4302537.5219242/1‑94 (MQ=60)
                                                CGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATC                                               <  SRR4302537.4932298/94‑1 (MQ=60)
                                                CGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATC                                               <  SRR4302537.5154030/94‑1 (MQ=60)
                                                 GGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCA                                              <  SRR4302537.4957830/94‑1 (MQ=60)
                                                 GGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCA                                              <  SRR4302537.4746574/94‑1 (MQ=60)
                                                  GATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAG                                             >  SRR4302537.1955110/1‑94 (MQ=60)
                                                   ATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGT                                            >  SRR4302537.1420630/1‑94 (MQ=60)
                                                   ATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGT                                            <  SRR4302537.2332312/94‑1 (MQ=60)
                                                   ATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGT                                            <  SRR4302537.2309592/94‑1 (MQ=60)
                                                    TCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTC                                           <  SRR4302537.4711711/94‑1 (MQ=60)
                                                     CGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCT                                          <  SRR4302537.586332/94‑1 (MQ=60)
                                                      GGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTT                                         <  SRR4302537.2639365/94‑1 (MQ=60)
                                                      GGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTT                                         >  SRR4302537.4207086/1‑94 (MQ=60)
                                                       GAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTT                                        <  SRR4302537.2892196/94‑1 (MQ=60)
                                                       GAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTT                                        <  SRR4302537.2994405/94‑1 (MQ=60)
                                                         AAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCGGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTAT                                      >  SRR4302537.5453642/1‑94 (MQ=60)
                                                         AAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTAT                                      <  SRR4302537.2476708/94‑1 (MQ=60)
                                                           ACAGAAGTCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGC                                    <  SRR4302537.6097445/94‑1 (MQ=60)
                                                           ACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGC                                    <  SRR4302537.3035301/94‑1 (MQ=60)
                                                            CAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCC                                   >  SRR4302537.1320567/1‑94 (MQ=60)
                                                            CAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCC                                   >  SRR4302537.1434151/1‑94 (MQ=60)
                                                             AGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCG                                  >  SRR4302537.3868317/1‑94 (MQ=60)
                                                             AGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCG                                  >  SRR4302537.3647749/1‑94 (MQ=60)
                                                             AGAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCG                                  <  SRR4302537.6430736/94‑1 (MQ=60)
                                                              GAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGG                                 <  SRR4302537.1263620/94‑1 (MQ=60)
                                                              GAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGG                                 >  SRR4302537.5008332/1‑94 (MQ=60)
                                                              GAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGG                                 <  SRR4302537.49167/94‑1 (MQ=60)
                                                              GAAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGG                                 <  SRR4302537.17758/94‑1 (MQ=60)
                                                               AAGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGT                                >  SRR4302537.4708840/1‑94 (MQ=60)
                                                                AGGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTT                               >  SRR4302537.4029846/1‑94 (MQ=60)
                                                                 GGCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTA                              >  SRR4302537.1295050/1‑94 (MQ=60)
                                                                  GCGGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTAT                             <  SRR4302537.1344005/94‑1 (MQ=60)
                                                                    GGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCA                           >  SRR4302537.866003/1‑94 (MQ=60)
                                                                    GGTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCA                           <  SRR4302537.595225/94‑1 (MQ=60)
                                                                     GTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAG                          >  SRR4302537.1013135/1‑94 (MQ=60)
                                                                     GTTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAG                          <  SRR4302537.581220/94‑1 (MQ=60)
                                                                      TTTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGG                         >  SRR4302537.3558699/1‑94 (MQ=60)
                                                                       TTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGC                        >  SRR4302537.4959353/1‑94 (MQ=60)
                                                                       TTAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGC                        <  SRR4302537.6343013/94‑1 (MQ=60)
                                                                        TAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCA                       <  SRR4302537.6409325/94‑1 (MQ=60)
                                                                        TAACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCA                       >  SRR4302537.3995731/1‑94 (MQ=60)
                                                                         AACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAG                      <  SRR4302537.4285276/94‑1 (MQ=60)
                                                                         AACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAG                      <  SRR4302537.5988862/94‑1 (MQ=60)
                                                                          ACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGG                     >  SRR4302537.4940573/1‑94 (MQ=60)
                                                                          ACAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGG                     <  SRR4302537.3493778/94‑1 (MQ=60)
                                                                           CAGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGGTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGA                    >  SRR4302537.1882406/1‑94 (MQ=60)
                                                                            AGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAA                   <  SRR4302537.5584904/94‑1 (MQ=60)
                                                                            AGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAA                   >  SRR4302537.6246322/1‑94 (MQ=60)
                                                                            AGAGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAA                   <  SRR4302537.3535640/94‑1 (MQ=60)
                                                                              AGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATA                 <  SRR4302537.744284/94‑1 (MQ=60)
                                                                              AGGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATA                 >  SRR4302537.3601210/1‑94 (MQ=60)
                                                                               GGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATAT                <  SRR4302537.6490150/94‑1 (MQ=60)
                                                                               GGAAAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATAT                <  SRR4302537.5571083/94‑1 (MQ=60)
                                                                                  AAGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGT             >  SRR4302537.5552733/1‑94 (MQ=60)
                                                                                   AGGCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTC            >  SRR4302537.5770748/1‑94 (MQ=60)
                                                                                     GCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAA          >  SRR4302537.6324214/1‑94 (MQ=60)
                                                                                     GCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAA          >  SRR4302537.796359/1‑94 (MQ=60)
                                                                                     GCTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAA          >  SRR4302537.6394408/1‑94 (MQ=60)
                                                                                      CTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGgcaaa         >  SRR4302537.4182618/1‑89 (MQ=60)
                                                                                      CTTATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAAC         >  SRR4302537.566704/1‑94 (MQ=60)
                                                                                        TATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACAT       >  SRR4302537.4090821/1‑94 (MQ=60)
                                                                                         ATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATC      <  SRR4302537.6195122/94‑1 (MQ=60)
                                                                                         ATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATC      >  SRR4302537.562495/1‑94 (MQ=60)
                                                                                         ATGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATC      >  SRR4302537.4916052/1‑94 (MQ=60)
                                                                                          TGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATCC     >  SRR4302537.4632316/1‑94 (MQ=60)
                                                                                          TGGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATCC     >  SRR4302537.983807/1‑94 (MQ=60)
                                                                                           GGTGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATCCC    <  SRR4302537.1954296/94‑1 (MQ=60)
                                                                                             TGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATCCCCA  <  SRR4302537.4661568/94‑1 (MQ=60)
                                                                                             TGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATCCCCA  <  SRR4302537.1114367/94‑1 (MQ=60)
                                                                                             TGACTGTAAAACGATGCACCCAAAGTTTGCCTTTGTCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATCCCCA  <  SRR4302537.4551516/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATAATTATTGAATTTTGCAGAAAAATGGAATTAGCTGCAAAAAAAGCACGGATCGGAAAACAGAAGGCGGTTTAACAGAGGAAAGGCTTATGGCGACTGTAAAACGATGCAGCCAAAGTTTGCCTTTGGCTGCAATGAAATCAGTCTTTATGCCGGTTATCAGGCAGGAATATGGTCAACATCCCCA  >  NC_000913/502560‑502746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑