Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 507,205 A→G F34L (TTT→CTT)  ybaP ← putative ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913507,2050AG97.9% 801.2 / 4.2 234F34L (TTT→CTT) ybaPputative ligase
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base G (142/87);  total (144/90)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 235 total aligned reads displayed.

GCGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAAATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTACA  >  NC_000913/507115‑507294
                                                                                          |                                                                                         
gcgcATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGAt                                                                                         <  2:5416947/93‑1 (MQ=255)
 cgcATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATg                                                                                        >  1:5332804/1‑93 (MQ=255)
 cgcATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATg                                                                                        <  1:6447173/93‑1 (MQ=255)
  gcATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATgg                                                                                       >  1:1258464/1‑93 (MQ=255)
    aTCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCg                                                                                     >  1:4517929/1‑93 (MQ=255)
     tCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGa                                                                                    >  1:3997466/1‑93 (MQ=255)
     tCGGCGTTTTTGAGCTTTTTAAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGa                                                                                    <  1:811901/93‑1 (MQ=255)
      cGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGAt                                                                                   <  1:4719741/93‑1 (MQ=255)
       ggCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGAtt                                                                                  >  1:2124498/1‑93 (MQ=255)
          gTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGcc                                                                               >  2:792498/1‑93 (MQ=255)
          gTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGcc                                                                               <  1:3730538/93‑1 (MQ=255)
           tttttgagctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCg                                                                              <  2:1227849/93‑1 (MQ=255)
           tttttgagctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCg                                                                              >  2:2156482/1‑93 (MQ=255)
           tttttgagctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCg                                                                              >  2:6158221/1‑93 (MQ=255)
           tttttgagctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCg                                                                              >  1:5891576/1‑93 (MQ=255)
           tttttgagctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCg                                                                              >  1:1069337/1‑93 (MQ=255)
            ttttgagctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCgg                                                                             >  1:4261088/1‑93 (MQ=255)
              ttgagctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCgggg                                                                           >  2:5815833/1‑93 (MQ=255)
               tgagctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGa                                                                          <  1:5604360/93‑1 (MQ=255)
               tgagctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGa                                                                          <  1:458208/93‑1 (MQ=255)
               tgagctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGa                                                                          >  2:6238087/1‑93 (MQ=255)
                gagctttttgagcAAACGGGTGTGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAg                                                                         <  1:6275975/93‑1 (MQ=255)
                 agctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAgg                                                                        <  1:4943784/93‑1 (MQ=255)
                  gctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGTAggg                                                                       <  1:1459364/93‑1 (MQ=255)
                  gctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAggg                                                                       >  1:5278499/1‑93 (MQ=255)
                  gctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAggg                                                                       <  1:4898941/93‑1 (MQ=255)
                  gctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAggg                                                                       >  2:4381899/1‑93 (MQ=255)
                   ctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCTATTGTCGGGGAgggg                                                                      >  2:1876967/1‑92 (MQ=255)
                   ctttttgagcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGt                                                                      >  1:1556303/1‑93 (MQ=255)
                           gcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGa                                                              >  1:5904764/1‑93 (MQ=255)
                           gcAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGa                                                              <  1:1978031/93‑1 (MQ=255)
                              aaCGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATgg                                                           >  1:1585142/1‑93 (MQ=255)
                               aCGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGc                                                          <  1:3921137/93‑1 (MQ=255)
                                 gggTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAg                                                        <  2:708528/93‑1 (MQ=255)
                                  ggTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAgg                                                       >  2:1197331/1‑93 (MQ=255)
                                  ggTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAgg                                                       <  1:2846148/93‑1 (MQ=255)
                                   gTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGc                                                      >  2:5126750/1‑93 (MQ=255)
                                   gTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGc                                                      >  1:1327627/1‑93 (MQ=255)
                                     gggCAGGGGAGCTATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCa                                                    <  1:969484/93‑1 (MQ=255)
                                       gCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAgg                                                  >  2:1413158/1‑93 (MQ=255)
                                        cAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAggg                                                 >  2:2409850/1‑92 (MQ=255)
                                         aGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGgtg                                                <  2:3263261/93‑1 (MQ=255)
                                         aGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGgtg                                                >  2:5991661/1‑93 (MQ=255)
                                          ggggAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGgtgt                                               >  2:224363/1‑93 (MQ=255)
                                          ggggAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGgtgt                                               >  1:377632/1‑93 (MQ=255)
                                          ggggAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGgtgt                                               <  1:4786520/93‑1 (MQ=255)
                                          ggggAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGgtgt                                               >  1:2929901/1‑93 (MQ=255)
                                             gAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAAt                                            >  2:4741489/1‑93 (MQ=255)
                                             gAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGGGATATCGATGGCAGGCCAGGTGTAAt                                            >  1:6245702/1‑93 (MQ=255)
                                             gAGCCATATCGTCGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAAt                                            <  2:1915421/93‑1 (MQ=255)
                                               gCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCAATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGa                                          <  2:1321860/93‑1 (MQ=255)
                                                ccATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGAt                                         >  2:4766623/1‑93 (MQ=255)
                                                ccATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGAt                                         >  2:3827466/1‑93 (MQ=255)
                                                ccATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGGAATGAt                                         >  1:3315275/1‑93 (MQ=255)
                                                 cATATCGTGGCTACCCATAAGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGAtt                                        >  2:6271104/1‑93 (MQ=255)
                                                  atatCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTa                                       >  1:1884686/1‑93 (MQ=255)
                                                   tatCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTAc                                      >  2:1022356/1‑93 (MQ=255)
                                                   tatCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTAc                                      >  1:5128826/1‑93 (MQ=255)
                                                   tatCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTAc                                      >  2:3609865/1‑93 (MQ=255)
                                                    atCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTAcc                                     >  1:5772439/1‑93 (MQ=255)
                                                    atCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTAcc                                     <  1:4618453/93‑1 (MQ=255)
                                                        tGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCgcgc                                 >  1:1188168/1‑93 (MQ=255)
                                                        tGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCgcgc                                 >  1:4774249/1‑93 (MQ=255)
                                                           cTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAgc                              <  2:1562994/93‑1 (MQ=255)
                                                            tACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAgcg                             >  1:5297506/1‑93 (MQ=255)
                                                            tACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGGGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAgcg                             >  1:3630561/1‑93 (MQ=255)
                                                                cATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGc                         <  1:879366/93‑1 (MQ=255)
                                                                cATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGc                         >  1:1651550/1‑93 (MQ=255)
                                                                cATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGc                         <  2:6428629/93‑1 (MQ=255)
                                                                 atatGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGcc                        >  2:6045188/1‑93 (MQ=255)
                                                                 atatGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGGGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGcc                        >  1:1753538/1‑93 (MQ=255)
                                                                  tatGAATACTGCCAATCAGATGAAAATGGCGATTGCCGGGGAGGGAGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGccc                       >  2:1108717/1‑93 (MQ=255)
                                                                   atGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCa                      >  2:4290041/1‑93 (MQ=255)
                                                                   atGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCa                      <  2:4224664/93‑1 (MQ=255)
                                                                    tGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCaa                     >  1:5036432/1‑93 (MQ=255)
                                                                    tGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCaa                     >  2:2320702/1‑93 (MQ=255)
                                                                    tGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCaa                     >  1:3169509/1‑93 (MQ=255)
                                                                     gAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCaaa                    <  2:4517929/93‑1 (MQ=255)
                                                                      aaTACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAg                   >  2:4953859/1‑93 (MQ=255)
                                                                      aaTACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAg                   >  2:2722073/1‑93 (MQ=255)
                                                                      aaTACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAg                   >  1:4693258/1‑93 (MQ=255)
                                                                      aaTACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAg                   >  1:384824/1‑93 (MQ=255)
                                                                       aTACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGc                  >  1:1139564/1‑93 (MQ=255)
                                                                       aTACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGc                  >  2:5856931/1‑93 (MQ=255)
                                                                         aCTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGt                <  2:2930351/93‑1 (MQ=255)
                                                                          cTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGtt               >  2:5033762/1‑93 (MQ=255)
                                                                           tGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGttt              >  1:242963/1‑93 (MQ=255)
                                                                           tGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGttt              >  1:2748926/1‑93 (MQ=255)
                                                                             ccAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGttttt            <  1:3524562/93‑1 (MQ=255)
                                                                              cAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTa           >  1:126152/1‑93 (MQ=255)
                                                                               aaTCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTAc          <  2:3008930/93‑1 (MQ=255)
                                                                                aTCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTAcc         <  1:1401549/93‑1 (MQ=255)
                                                                                aTCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTAcc         >  2:1681661/1‑93 (MQ=255)
                                                                                  cAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTAccag       <  2:4072298/93‑3 (MQ=255)
                                                                                   aGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCgg      >  1:2731399/1‑93 (MQ=255)
                                                                                   aGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCgg      >  1:5188956/1‑93 (MQ=255)
                                                                                     aTGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTa    <  1:5555431/93‑1 (MQ=255)
                                                                                       gAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTaca  >  1:681764/1‑93 (MQ=255)
                                                                                       gAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGGAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTaca  >  2:3501157/1‑93 (MQ=255)
                                                                                       gAAGATGGCGATTGCCGGGGAGGGCGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTaca  >  1:435262/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAAATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTACA  >  NC_000913/507115‑507294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGCGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAAATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTACAACAG  >  NC_000913/507112‑507298
                                                                                             |                                                                                             
TCAGCGCATCGGCGTTTTTGAGCTTTTGGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAG                                                                                               <  SRR4302537.2733135/94‑1 (MQ=60)
 CAGCGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGA                                                                                              <  SRR4302537.568307/94‑1 (MQ=60)
 CAGCGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGA                                                                                              >  SRR4302537.934564/1‑94 (MQ=60)
 CAGCGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGA                                                                                              >  SRR4302537.3750242/1‑94 (MQ=60)
   GCGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATG                                                                                            >  SRR4302537.5369379/1‑94 (MQ=60)
    CGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGG                                                                                           >  SRR4302537.156831/1‑94 (MQ=60)
    CGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGG                                                                                           >  SRR4302537.4900309/1‑94 (MQ=60)
    CGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGG                                                                                           <  SRR4302537.4729881/94‑1 (MQ=60)
    CGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGG                                                                                           <  SRR4302537.4679723/94‑1 (MQ=60)
    CGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGG                                                                                           >  SRR4302537.1267222/1‑94 (MQ=60)
    CGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGG                                                                                           <  SRR4302537.6490905/94‑1 (MQ=60)
    CGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAAATGG                                                                                           <  SRR4302537.4808800/94‑1 (MQ=60)
     GCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGC                                                                                          <  SRR4302537.909481/94‑1 (MQ=60)
      CATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCG                                                                                         >  SRR4302537.4549182/1‑94 (MQ=60)
      CATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCG                                                                                         >  SRR4302537.4401745/1‑94 (MQ=60)
       ATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGA                                                                                        >  SRR4302537.4025452/1‑94 (MQ=60)
       ATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGA                                                                                        >  SRR4302537.5331727/1‑94 (MQ=60)
        TCGGCGTTTTTGAGCTTTTTAAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGAT                                                                                       <  SRR4302537.817466/94‑1 (MQ=60)
         CGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATT                                                                                      <  SRR4302537.4752376/94‑1 (MQ=60)
         CGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATT                                                                                      >  SRR4302537.2139442/1‑94 (MQ=60)
          GGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTG                                                                                     <  SRR4302537.3650324/94‑1 (MQ=60)
          GGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTG                                                                                     >  SRR4302537.1680762/1‑94 (MQ=60)
            CGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCC                                                                                   >  SRR4302537.2311060/1‑94 (MQ=60)
            CGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAAATGGCGATTGCC                                                                                   >  SRR4302537.3133263/1‑94 (MQ=60)
             GTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCG                                                                                  >  SRR4302537.1076708/1‑94 (MQ=60)
             GTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCG                                                                                  >  SRR4302537.5209639/1‑94 (MQ=60)
             GTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCG                                                                                  <  SRR4302537.3756743/94‑1 (MQ=60)
             GTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCG                                                                                  >  SRR4302537.2152226/1‑94 (MQ=60)
             GTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCG                                                                                  >  SRR4302537.4038101/1‑94 (MQ=60)
             GTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCG                                                                                  >  SRR4302537.5932001/1‑94 (MQ=60)
              TTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGG                                                                                 >  SRR4302537.4290697/1‑94 (MQ=60)
               TTTTGAGATTTATGAGCAAACGGGTGGGCAGGGTAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGG                                                                                <  SRR4302537.3869825/94‑1 (MQ=60)
                TTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGG                                                                               <  SRR4302537.1961060/94‑1 (MQ=60)
                  TGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAG                                                                             <  SRR4302537.5642676/94‑1 (MQ=60)
                  TGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAG                                                                             >  SRR4302537.5840618/1‑94 (MQ=60)
                  TGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAG                                                                             <  SRR4302537.461368/94‑1 (MQ=60)
                   GAGCTTTTTGAGCAAACGGGTGTGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGG                                                                            <  SRR4302537.6319033/94‑1 (MQ=60)
                   GAGCTTTTTGAGCAAACGGGTGTGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGG                                                                            <  SRR4302537.6319034/94‑1 (MQ=60)
                   GAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGG                                                                            <  SRR4302537.6326771/94‑1 (MQ=60)
                   GAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGG                                                                            <  SRR4302537.1635423/94‑1 (MQ=60)
                    AGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGG                                                                           >  SRR4302537.4007214/1‑94 (MQ=60)
                    AGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGG                                                                           >  SRR4302537.5314813/1‑94 (MQ=60)
                    AGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGG                                                                           <  SRR4302537.4977874/94‑1 (MQ=60)
                    AGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGG                                                                           >  SRR4302537.4185207/1‑94 (MQ=60)
                    AGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATACGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGG                                                                           >  SRR4302537.1569264/1‑94 (MQ=60)
                     GCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGTAGGGT                                                                          <  SRR4302537.1469521/94‑1 (MQ=60)
                     GCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGT                                                                          >  SRR4302537.1567165/1‑94 (MQ=60)
                     GCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGT                                                                          <  SRR4302537.4932757/94‑1 (MQ=60)
                       TTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGGGATTGCCGGGGAGGGTGA                                                                        >  SRR4302537.1547381/1‑94 (MQ=60)
                       TTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGA                                                                        >  SRR4302537.5299047/1‑94 (MQ=60)
                         TTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATA                                                                      >  SRR4302537.593494/1‑94 (MQ=60)
                          taaagaaaacaGGTGGGAAGGGGAGCGATATCGTGGCTACCCATATGAATACTGCCAATAAGATGAAGATGGCGATTGCCGGGGAGGGTGATAT                                                                     <  SRR4302537.596440/83‑1 (MQ=60)
                          TTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATAT                                                                     <  SRR4302537.2755921/94‑1 (MQ=60)
                           TGNGNNNANGGGTGGGCAGNGNNNCCATATCGTGGCTACCCANATNAATACTGCCAATCAGATGAAAATGGCGATTNCCGGGGAGGGTGATATC                                                                    <  SRR4302537.4896418/94‑1 (MQ=60)
                             AGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGA                                                                  >  SRR4302537.5945282/1‑94 (MQ=60)
                              GCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGAT                                                                 <  SRR4302537.1991776/94‑1 (MQ=60)
                              GCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGAG                                                                 >  SRR4302537.1312959/1‑94 (MQ=60)
                               CAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATG                                                                <  SRR4302537.4991801/94‑1 (MQ=60)
                                AAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGG                                                               >  SRR4302537.1596223/1‑94 (MQ=60)
                                AAACGGGGGGCAAGGGGAGCCAGATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGG                                                               <  SRR4302537.4633165/94‑1 (MQ=60)
                                 AACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGCGATATCGATGGC                                                              >  SRR4302537.5627744/1‑94 (MQ=60)
                                  ACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCA                                                             <  SRR4302537.3948543/94‑1 (MQ=60)
                                   CGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAG                                                            <  SRR4302537.459136/94‑1 (MQ=60)
                                   CGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAG                                                            >  SRR4302537.4751793/1‑94 (MQ=60)
                                     GGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGC                                                          >  SRR4302537.1336887/1‑94 (MQ=60)
                                     GGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGC                                                          <  SRR4302537.3595128/94‑1 (MQ=60)
                                     GGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGC                                                          <  SRR4302537.2866073/94‑1 (MQ=60)
                                      GTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCC                                                         <  SRR4302537.2775823/94‑1 (MQ=60)
                                        GGGCAGGGGAGCTATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAG                                                       <  SRR4302537.976166/94‑1 (MQ=60)
                                        GGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAG                                                       >  SRR4302537.994000/1‑94 (MQ=60)
                                           GAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTG                                                    <  SRR4302537.1798916/94‑1 (MQ=60)
                                           CAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTG                                                    <  SRR4302537.4362264/94‑1 (MQ=60)
                                            AGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGT                                                   >  SRR4302537.2950475/1‑94 (MQ=60)
                                            AGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGT                                                   >  SRR4302537.380250/1‑94 (MQ=60)
                                             GGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTA                                                  <  SRR4302537.4819555/94‑1 (MQ=60)
                                               GGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGGGATATCGATGGCAGGCCAGGTGTAAT                                                >  SRR4302537.6288624/1‑94 (MQ=60)
                                                  GCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTCATGAT                                             <  SRR4302537.650561/94‑1 (MQ=60)
                                                  GCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGGAATGAT                                             >  SRR4302537.3338727/1‑94 (MQ=60)
                                                    CATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTA                                           >  SRR4302537.1897794/1‑94 (MQ=60)
                                                    CATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTA                                           >  SRR4302537.4955099/1‑94 (MQ=60)
                                                    CATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTA                                           >  SRR4302537.756111/1‑94 (MQ=60)
                                                     ATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTAC                                          >  SRR4302537.5164169/1‑94 (MQ=60)
                                                     ATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTAC                                          <  SRR4302537.5131262/94‑1 (MQ=60)
                                                      TATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACC                                         >  SRR4302537.5812002/1‑94 (MQ=60)
                                                      TATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACC                                         >  SRR4302537.2771870/1‑94 (MQ=60)
                                                       ATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCG                                        <  SRR4302537.4650439/94‑1 (MQ=60)
                                                          GTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGC                                     >  SRR4302537.1196422/1‑94 (MQ=60)
                                                          GTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGC                                     >  SRR4302537.1358731/1‑94 (MQ=60)
                                                          GTGGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGC                                     >  SRR4302537.4807211/1‑94 (MQ=60)
                                                            GGCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAG                                   <  SRR4302537.4565848/94‑1 (MQ=60)
                                                             GTTACCCATATGAATACTGCCAATCAGATGAAGAGGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGC                                  <  SRR4302537.3029108/94‑1 (MQ=60)
                                                             GCTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGC                                  <  SRR4302537.3884047/94‑1 (MQ=60)
                                                              CTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCG                                 >  SRR4302537.4786722/1‑94 (MQ=60)
                                                              CTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCG                                 >  SRR4302537.5333896/1‑94 (MQ=60)
                                                              CTACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGGGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCG                                 >  SRR4302537.3656144/1‑94 (MQ=60)
                                                                ACCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCG                               <  SRR4302537.3794267/94‑1 (MQ=60)
                                                                 CCCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGG                              >  SRR4302537.1207062/1‑94 (MQ=60)
                                                                  CCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGC                             >  SRR4302537.54562/1‑94 (MQ=60)
                                                                  CCATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGC                             >  SRR4302537.1663131/1‑94 (MQ=60)
                                                                   CATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCC                            <  SRR4302537.885418/94‑1 (MQ=60)
                                                                   CATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGACATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCC                            >  SRR4302537.6225523/1‑94 (MQ=60)
                                                                   CATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGGGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCC                            >  SRR4302537.1765763/1‑94 (MQ=60)
                                                                   CATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGAGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCC                            >  SRR4302537.1822964/1‑94 (MQ=60)
                                                                    ATATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGGGTAATGATTACCGCGCAGCGCGGCCA                           >  SRR4302537.2059290/1‑94 (MQ=60)
                                                                      ATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAA                         >  SRR4302537.3191776/1‑94 (MQ=60)
                                                                      ATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAA                         >  SRR4302537.5071104/1‑94 (MQ=60)
                                                                      ATGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAA                         >  SRR4302537.773816/1‑94 (MQ=60)
                                                                       TGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAA                        <  SRR4302537.5347476/94‑1 (MQ=60)
                                                                       TGAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAA                        <  SRR4302537.287908/94‑1 (MQ=60)
                                                                        GAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAG                       >  SRR4302537.387487/1‑94 (MQ=60)
                                                                        GAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAG                       >  SRR4302537.4725720/1‑94 (MQ=60)
                                                                        GAATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAG                       <  SRR4302537.4999177/94‑1 (MQ=60)
                                                                         AATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGC                      >  SRR4302537.1147480/1‑94 (MQ=60)
                                                                         AATACTGCCAATCAGATGAAAATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGC                      <  SRR4302537.851556/94‑1 (MQ=60)
                                                                          ATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCG                     >  SRR4302537.5882783/1‑94 (MQ=60)
                                                                          ATACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCG                     >  SRR4302537.1192595/1‑94 (MQ=60)
                                                                           TACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGT                    >  SRR4302537.2470191/1‑94 (MQ=60)
                                                                            ACTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTT                   >  SRR4302537.1137862/1‑94 (MQ=60)
                                                                             CTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTT                  >  SRR4302537.244684/1‑94 (MQ=60)
                                                                             CTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTT                  <  SRR4302537.141881/94‑1 (MQ=60)
                                                                             CTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTT                  <  SRR4302537.5283360/94‑1 (MQ=60)
                                                                             CTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTT                  >  SRR4302537.2768100/1‑94 (MQ=60)
                                                                             CTGCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTT                  <  SRR4302537.1268080/94‑1 (MQ=60)
                                                                               GCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCATAGCGATTTT                >  SRR4302537.3754602/1‑94 (MQ=60)
                                                                               GCCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTT                >  SRR4302537.4893395/1‑94 (MQ=60)
                                                                                CCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTA               >  SRR4302537.3351093/1‑94 (MQ=60)
                                                                                CCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTA               <  SRR4302537.3549494/94‑1 (MQ=60)
                                                                                CCAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTA               >  SRR4302537.127057/1‑94 (MQ=60)
                                                                                 CAATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTAC              >  SRR4302537.5382306/1‑94 (MQ=60)
                                                                                   ATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCC            <  SRR4302537.1411316/94‑1 (MQ=60)
                                                                                   ATCAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCC            <  SRR4302537.6306809/94‑1 (MQ=60)
                                                                                   ATCAGATGAAGATGGCGATTGCCGGGGAGGGGGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCC            >  SRR4302537.380261/1‑94 (MQ=60)
                                                                                     CAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGG          >  SRR4302537.5224647/1‑94 (MQ=60)
                                                                                     CAGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGG          >  SRR4302537.2750465/1‑94 (MQ=60)
                                                                                     CAGATGAAAATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGG          <  SRR4302537.4041001/94‑1 (MQ=60)
                                                                                      AGATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGT         <  SRR4302537.319928/94‑1 (MQ=60)
                                                                                        ATGAAGATTGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTAC       <  SRR4302537.1345526/94‑1 (MQ=60)
                                                                                        ATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTAC       <  SRR4302537.5593361/94‑1 (MQ=60)
                                                                                        ATGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTAC       <  SRR4302537.5781356/94‑1 (MQ=60)
                                                                                         TGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTACA      >  SRR4302537.686434/1‑94 (MQ=60)
                                                                                         TGAAGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTACA      <  SRR4302537.1266067/94‑1 (MQ=60)
                                                                                         TGAAGATGGCGATTGCCGGGGAGGGCGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTACA      >  SRR4302537.438297/1‑94 (MQ=60)
                                                                                            AGATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCACAGCGTTTTTACCCGGTACAACA   >  SRR4302537.3706091/1‑94 (MQ=60)
                                                                                             GATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTACAACAG  >  SRR4302537.2952890/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCAGCGCATCGGCGTTTTTGAGCTTTTTGAGCAAACGGGTGGGCAGGGGAGCCATATCGTGGCTACCCATATGAATACTGCCAATCAGATGAAAATGGCGATTGCCGGGGAGGGTGATATCGATGGCAGGCCAGGTGTAATGATTACCGCGCAGCGCGGCCCAAAGCGTTTTTACCCGGTACAACAG  >  NC_000913/507112‑507298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑