Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 586,139 T→C intergenic (‑8/+175) envY ← / ← ybcH envelope protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913586,1390TC94.9% 801.9 / 23.4 258intergenic (‑8/+175)envY/ybcHenvelope protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (6/7);  new base C (109/135);  total (116/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 269 total aligned reads displayed.

TAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCAC  >  NC_000913/586047‑586230
                                                                                            |                                                                                           
   aCGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCAcccct                                                                                          <  1:5128376/93‑1 (MQ=255)
    cGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCAcccctc                                                                                         >  1:232842/1‑93 (MQ=255)
    cGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCAcccctc                                                                                         >  1:2384169/1‑93 (MQ=255)
        gCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGat                                                                                     >  2:5456865/1‑93 (MQ=255)
        gCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGat                                                                                     >  1:202164/1‑93 (MQ=255)
         cATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGata                                                                                    <  1:5270177/93‑1 (MQ=255)
         cATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGata                                                                                    >  1:3198354/1‑93 (MQ=255)
          aTGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGatat                                                                                   >  2:1584116/1‑93 (MQ=255)
          aTGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGatat                                                                                   >  2:4682954/1‑93 (MQ=255)
          aTGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGatat                                                                                   >  2:3054392/1‑93 (MQ=255)
           tGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATc                                                                                  <  1:4301294/93‑1 (MQ=255)
            ggTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCa                                                                                 <  2:944079/93‑1 (MQ=255)
            ggTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCa                                                                                 <  2:3813894/93‑1 (MQ=255)
            ggTTATAGACGCTTTCCTCTACCTGTTTTTCGGTCAGGATCCCCCCGCAAGTTTCACTGCTGCTCAATTGCAGTTCGCCCCCCTCAGATATCa                                                                                 <  2:5186395/93‑1 (MQ=255)
             gTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAg                                                                                >  1:5486116/1‑93 (MQ=255)
             gTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAg                                                                                <  2:1851906/93‑1 (MQ=255)
             gTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAg                                                                                >  1:3072232/1‑93 (MQ=255)
             gTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAg                                                                                >  1:5950560/1‑93 (MQ=255)
             gTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAg                                                                                <  2:1379528/93‑1 (MQ=255)
                aTTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGaaa                                                                             >  2:4805275/1‑93 (MQ=255)
                 ttGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAAc                                                                            <  1:2664818/93‑1 (MQ=255)
                 ttGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAAc                                                                            <  2:2664198/93‑1 (MQ=255)
                  tGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACt                                                                           <  1:6368798/93‑1 (MQ=255)
                   gACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTc                                                                          <  2:6162845/93‑1 (MQ=255)
                    aCGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTcc                                                                         <  1:5717233/93‑1 (MQ=255)
                     cGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCg                                                                        >  2:2972285/1‑93 (MQ=255)
                       cTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCt                                                                      >  1:883755/1‑93 (MQ=255)
                         tacctctacctcTTTTTCGGCCAGGATCAGCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATGAGGAACTCCGCTCa                                                                    >  2:1608871/1‑93 (MQ=255)
                             tctacctctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAgg                                                                <  2:3861474/93‑1 (MQ=255)
                             tctacctctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAgg                                                                >  1:5557207/1‑93 (MQ=255)
                             tctacctctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAgg                                                                >  1:1071297/1‑93 (MQ=255)
                              ctacctctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGa                                                               >  1:1835075/1‑93 (MQ=255)
                               tacctctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGAt                                                              >  1:1442235/1‑93 (MQ=255)
                               tacctctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGAt                                                              <  1:1903080/93‑1 (MQ=255)
                                 cctctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCt                                                            >  2:5102560/1‑93 (MQ=255)
                                  ctctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTa                                                           >  1:469899/1‑93 (MQ=255)
                                  ctctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTa                                                           <  2:6344315/93‑1 (MQ=255)
                                  ctctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTa                                                           <  2:4188961/93‑1 (MQ=255)
                                  ctctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTa                                                           <  2:884651/93‑1 (MQ=255)
                                  ctctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACACCTCAGATATCAGAAACTCCGCTCAAAGGATCTa                                                           >  2:3440450/1‑93 (MQ=255)
                                   tctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTAt                                                          <  1:1348328/93‑1 (MQ=255)
                                    ctTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATg                                                         >  1:4658672/1‑93 (MQ=255)
                                        ttCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTc                                                     <  2:5810178/93‑1 (MQ=255)
                                         tCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTcc                                                    <  1:5048815/93‑1 (MQ=255)
                                         tCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTcc                                                    <  2:5497431/93‑1 (MQ=255)
                                          cGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCt                                                   <  1:3916838/93‑1 (MQ=255)
                                           ggTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTg                                                  >  1:5214083/1‑93 (MQ=255)
                                            gTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGc                                                 >  1:2527062/1‑93 (MQ=255)
                                             tCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCa                                                >  2:2138588/1‑93 (MQ=255)
                                             tCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCa                                                <  1:66815/93‑1 (MQ=255)
                                              cAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCAt                                               >  2:2449187/1‑93 (MQ=255)
                                               aGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATg                                              >  1:2642445/1‑93 (MQ=255)
                                                ggATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGa                                             >  2:278886/1‑93 (MQ=255)
                                                ggATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGAGATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGa                                             <  2:3165124/93‑1 (MQ=255)
                                                 gATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAg                                            >  2:3873993/1‑93 (MQ=255)
                                                   tCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTg                                          >  1:4859700/1‑93 (MQ=255)
                                                      ccacGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATc                                       <  1:3962280/93‑1 (MQ=255)
                                                      ccacGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATc                                       <  2:4393235/93‑1 (MQ=255)
                                                          gCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGcc                                   <  1:945163/93‑1 (MQ=255)
                                                           cAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGccc                                  >  1:2196768/1‑93 (MQ=255)
                                                           cAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGccc                                  >  1:4458885/1‑93 (MQ=255)
                                                             aGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGt                                <  2:4291992/93‑1 (MQ=255)
                                                              ggTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGtt                               <  2:3680832/93‑1 (MQ=255)
                                                              ggTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGtt                               >  1:3409831/1‑93 (MQ=255)
                                                               gTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTc                              <  1:712082/93‑1 (MQ=255)
                                                               gTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTc                              <  2:5747981/93‑1 (MQ=255)
                                                                 tCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGc                            >  1:4218045/1‑93 (MQ=255)
                                                                   aCTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCg                          >  2:2721633/1‑93 (MQ=255)
                                                                    ctgctgCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGa                         >  2:568600/1‑93 (MQ=255)
                                                                    ctgctgCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGa                         <  2:751408/93‑1 (MQ=255)
                                                                     tgctgcTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGAt                        >  2:1933767/1‑93 (MQ=255)
                                                                      gctgctCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATa                       >  2:4612222/1‑93 (MQ=255)
                                                                      gctgctCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATa                       >  2:1534315/1‑93 (MQ=255)
                                                                       ctgctCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATaa                      <  1:1039010/93‑1 (MQ=255)
                                                                         gctCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACg                    <  2:5960024/93‑1 (MQ=255)
                                                                         gctCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACg                    <  2:1005259/93‑1 (MQ=255)
                                                                         gctCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACg                    >  1:5870270/1‑93 (MQ=255)
                                                                          ctCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGa                   <  1:6025572/93‑1 (MQ=255)
                                                                          ctCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGa                   >  1:2975071/1‑93 (MQ=255)
                                                                           tCAATTGCATTTCGCCCCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAt                  <  1:1225590/93‑1 (MQ=255)
                                                                            cAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAtc                 <  2:2314682/93‑1 (MQ=255)
                                                                            cAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAtc                 <  2:5056568/93‑1 (MQ=255)
                                                                             aaTTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAtct                <  1:5398904/93‑1 (MQ=255)
                                                                             aaTTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAtct                <  2:2464122/93‑1 (MQ=255)
                                                                             aaTTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAtct                <  1:3974783/93‑1 (MQ=255)
                                                                              aTTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAtctt               >  1:3487193/1‑93 (MQ=255)
                                                                              aTTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAtctt               >  1:2826252/1‑93 (MQ=255)
                                                                              aTTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAtctt               >  2:6044577/1‑93 (MQ=255)
                                                                               ttGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAtcttc              >  2:2758279/1‑93 (MQ=255)
                                                                                tGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAtcttct             <  1:5678372/93‑1 (MQ=255)
                                                                                 gCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATcttctt            >  1:3744372/1‑93 (MQ=255)
                                                                                 gCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATcttctt            <  1:6223128/93‑1 (MQ=255)
                                                                                   aTTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCttctttc          <  2:1243737/93‑1 (MQ=255)
                                                                                   aTTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCttctttc          >  2:3624863/1‑93 (MQ=255)
                                                                                   aTTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCttctttc          >  2:4015164/1‑93 (MQ=255)
                                                                                   aTTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCttctttc          <  1:2722964/93‑1 (MQ=255)
                                                                                       cGCACCCCTCAGATATCATAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTa      <  2:1135388/93‑1 (MQ=255)
                                                                                       cGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGTGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTa      <  2:646336/93‑1 (MQ=255)
                                                                                        gCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAg     <  2:2334906/93‑1 (MQ=255)
                                                                                         cACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGc    <  2:528826/93‑1 (MQ=255)
                                                                                            |                                                                                           
TAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCAC  >  NC_000913/586047‑586230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGC  >  NC_000913/586046‑586232
                                                                                             |                                                                                             
GTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACC                                                                                               >  SRR4302537.3060795/1‑94 (MQ=60)
GTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACC                                                                                               <  SRR4302537.5913812/94‑1 (MQ=60)
 TAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCC                                                                                              >  SRR4302537.4413207/1‑94 (MQ=60)
   AACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCT                                                                                            >  SRR4302537.2426641/1‑94 (MQ=60)
    ACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTC                                                                                           >  SRR4302537.2400964/1‑94 (MQ=60)
    ACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTC                                                                                           >  SRR4302537.234486/1‑94 (MQ=60)
    ACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTC                                                                                           <  SRR4302537.5163718/94‑1 (MQ=60)
     CGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCA                                                                                          <  SRR4302537.1273311/94‑1 (MQ=60)
      GTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAG                                                                                         >  SRR4302537.1409668/1‑94 (MQ=60)
        AGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCGGAT                                                                                       <  SRR4302537.4714928/94‑1 (MQ=60)
        AGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGAT                                                                                       >  SRR4302537.203609/1‑94 (MQ=60)
         GCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATA                                                                                      >  SRR4302537.3220844/1‑94 (MQ=60)
         GCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATA                                                                                      <  SRR4302537.5907137/94‑1 (MQ=60)
          CATGGTTGTTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATAT                                                                                     <  SRR4302537.1143701/94‑1 (MQ=60)
          CATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATAT                                                                                     >  SRR4302537.999681/1‑94 (MQ=60)
          CATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATAT                                                                                     >  SRR4302537.4302821/1‑94 (MQ=60)
          CATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATAT                                                                                     <  SRR4302537.5306449/94‑1 (MQ=60)
           ATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATC                                                                                    >  SRR4302537.3977559/1‑94 (MQ=60)
            TGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCA                                                                                   <  SRR4302537.3006846/94‑1 (MQ=60)
            TGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCA                                                                                   <  SRR4302537.4331186/94‑1 (MQ=60)
            TGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCA                                                                                   <  SRR4302537.3158441/94‑1 (MQ=60)
            TGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCA                                                                                   <  SRR4302537.2420841/94‑1 (MQ=60)
             GGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAG                                                                                  >  SRR4302537.5523564/1‑94 (MQ=60)
             GGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAG                                                                                  >  SRR4302537.3093633/1‑94 (MQ=60)
             GGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAG                                                                                  >  SRR4302537.5991409/1‑94 (MQ=60)
               TTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAA                                                                                >  SRR4302537.5962428/1‑94 (MQ=60)
                 ATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAAC                                                                              >  SRR4302537.5264169/1‑94 (MQ=60)
                 ATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAAC                                                                              >  SRR4302537.2222688/1‑94 (MQ=60)
                  TTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACT                                                                             <  SRR4302537.2683432/94‑1 (MQ=60)
                   TGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTC                                                                            <  SRR4302537.6412234/94‑1 (MQ=60)
                   TGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTC                                                                            <  SRR4302537.5530554/94‑1 (MQ=60)
                     ACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCG                                                                          <  SRR4302537.5756362/94‑1 (MQ=60)
                       GCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCT                                                                        >  SRR4302537.3889938/1‑94 (MQ=60)
                       GCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCT                                                                        >  SRR4302537.889836/1‑94 (MQ=60)
                            CCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAG                                                                   >  SRR4302537.4049434/1‑94 (MQ=60)
                            CCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAG                                                                   >  SRR4302537.5238525/1‑94 (MQ=60)
                            CCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAG                                                                   <  SRR4302537.25956/94‑1 (MQ=60)
                             CTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGG                                                                  <  SRR4302537.899312/94‑1 (MQ=60)
                             CTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGG                                                                  >  SRR4302537.5743066/1‑94 (MQ=60)
                             CTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGG                                                                  >  SRR4302537.1078684/1‑94 (MQ=60)
                             CTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGG                                                                  >  SRR4302537.5595178/1‑94 (MQ=60)
                              TCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGA                                                                 >  SRR4302537.1847850/1‑94 (MQ=60)
                               CTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGAT                                                                >  SRR4302537.1452284/1‑94 (MQ=60)
                                TACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATC                                                               <  SRR4302537.1916297/94‑1 (MQ=60)
                                TACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATC                                                               >  SRR4302537.2267748/1‑94 (MQ=60)
                                TACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATC                                                               <  SRR4302537.2063885/94‑1 (MQ=60)
                                  CCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTA                                                             >  SRR4302537.473119/1‑94 (MQ=60)
                                  CCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTA                                                             <  SRR4302537.534215/94‑1 (MQ=60)
                                   CTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTAT                                                            <  SRR4302537.4505662/94‑1 (MQ=60)
                                    TCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATG                                                           >  SRR4302537.4690880/1‑94 (MQ=60)
                                    TCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATG                                                           <  SRR4302537.1357725/94‑1 (MQ=60)
                                     CTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGC                                                          >  SRR4302537.5993480/1‑94 (MQ=60)
                                      TTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCT                                                         <  SRR4302537.3499174/94‑1 (MQ=60)
                                       TTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTT                                                        <  SRR4302537.4954123/94‑1 (MQ=60)
                                       TTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTT                                                        >  SRR4302537.5970492/1‑94 (MQ=60)
                                          TCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCT                                                     >  SRR4302537.18984/1‑94 (MQ=60)
                                          TCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCT                                                     <  SRR4302537.5083579/94‑1 (MQ=60)
                                          TCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCT                                                     <  SRR4302537.2732357/94‑1 (MQ=60)
                                           CGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAACCTCCGCTCAAAGGATCTATGCTTCCTG                                                    <  SRR4302537.755737/94‑1 (MQ=60)
                                           CGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTG                                                    <  SRR4302537.3944215/94‑1 (MQ=60)
                                           CGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTG                                                    <  SRR4302537.5396111/94‑1 (MQ=60)
                                           CGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTG                                                    <  SRR4302537.6366957/94‑1 (MQ=60)
                                           CGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTG                                                    <  SRR4302537.190610/94‑1 (MQ=60)
                                           CGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTG                                                    >  SRR4302537.5249936/1‑94 (MQ=60)
                                            GGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGC                                                   >  SRR4302537.2544793/1‑94 (MQ=60)
                                            GGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGC                                                   >  SRR4302537.5571298/1‑94 (MQ=60)
                                              TCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCAT                                                 <  SRR4302537.67309/94‑1 (MQ=60)
                                              TCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCAT                                                 <  SRR4302537.4334600/94‑1 (MQ=60)
                                               CAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATG                                                >  SRR4302537.2660933/1‑94 (MQ=60)
                                                AGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGA                                               >  SRR4302537.3118928/1‑94 (MQ=60)
                                                 GGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAG                                              <  SRR4302537.3270160/94‑1 (MQ=60)
                                                  GATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGT                                             <  SRR4302537.648778/94‑1 (MQ=60)
                                                   ATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTG                                            <  SRR4302537.1312966/94‑1 (MQ=60)
                                                   ATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTG                                            >  SRR4302537.4893227/1‑94 (MQ=60)
                                                   ATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTG                                            <  SRR4302537.1651292/94‑1 (MQ=60)
                                                   ATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTGCGCACCCATCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTG                                            <  SRR4302537.2804432/94‑1 (MQ=60)
                                                    TCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGA                                           >  SRR4302537.6396870/1‑94 (MQ=60)
                                                    TCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGA                                           <  SRR4302537.4598510/94‑1 (MQ=60)
                                                     CACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGAT                                          <  SRR4302537.6144857/94‑1 (MQ=60)
                                                      ACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATC                                         <  SRR4302537.3851499/94‑1 (MQ=60)
                                                      ACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATC                                         >  SRR4302537.4454336/1‑94 (MQ=60)
                                                       CCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCG                                        <  SRR4302537.3989999/94‑1 (MQ=60)
                                                       CCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCG                                        <  SRR4302537.3989998/94‑1 (MQ=60)
                                                           GCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCC                                    <  SRR4302537.5125930/94‑1 (MQ=60)
                                                           GCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCC                                    <  SRR4302537.951698/94‑1 (MQ=60)
                                                           GCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCC                                    <  SRR4302537.896814/94‑1 (MQ=60)
                                                           GCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCC                                    >  SRR4302537.4489716/1‑94 (MQ=60)
                                                           GCAAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCC                                    >  SRR4302537.2212247/1‑94 (MQ=60)
                                                             AAGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGT                                  >  SRR4302537.1962997/1‑94 (MQ=60)
                                                              AGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTT                                 >  SRR4302537.4090386/1‑94 (MQ=60)
                                                              AGGTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTT                                 >  SRR4302537.3433984/1‑94 (MQ=60)
                                                               GCTTCACTGCTGCTCAATTGCATTTCGCACCCATCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTC                                <  SRR4302537.2561950/94‑1 (MQ=60)
                                                                GTTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCG                               <  SRR4302537.716957/94‑1 (MQ=60)
                                                                 TTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGC                              <  SRR4302537.4768624/94‑1 (MQ=60)
                                                                 TTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGC                              <  SRR4302537.5481609/94‑1 (MQ=60)
                                                                 TTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGC                              >  SRR4302537.4247445/1‑94 (MQ=60)
                                                                 TTCACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGC                              <  SRR4302537.5708987/94‑1 (MQ=60)
                                                                   TACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCG                            <  SRR4302537.2763710/94‑1 (MQ=60)
                                                                   CACTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCG                            <  SRR4302537.6224793/94‑1 (MQ=60)
                                                                     CTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGAT                          <  SRR4302537.1081215/94‑1 (MQ=60)
                                                                     CTGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGAT                          >  SRR4302537.4722143/1‑94 (MQ=60)
                                                                      TGCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATA                         >  SRR4302537.5091637/1‑94 (MQ=60)
                                                                       GCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAA                        <  SRR4302537.1071705/94‑1 (MQ=60)
                                                                       GCTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAA                        <  SRR4302537.2356521/94‑1 (MQ=60)
                                                                        CTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAAC                       >  SRR4302537.2666226/1‑94 (MQ=60)
                                                                        CTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAAC                       <  SRR4302537.2689187/94‑1 (MQ=60)
                                                                        CTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAAC                       <  SRR4302537.6519806/94‑1 (MQ=60)
                                                                        CTGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAAC                       <  SRR4302537.1046161/94‑1 (MQ=60)
                                                                         TGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACG                      <  SRR4302537.3766304/94‑1 (MQ=60)
                                                                         TGCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACG                      >  SRR4302537.5910538/1‑94 (MQ=60)
                                                                          GCTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGA                     >  SRR4302537.2995902/1‑94 (MQ=60)
                                                                           CTCAATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGAT                    <  SRR4302537.6066976/94‑1 (MQ=60)
                                                                            TCAATTGCATTTCGCCCCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATC                   <  SRR4302537.1234108/94‑1 (MQ=60)
                                                                              AATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTT                 <  SRR4302537.5435757/94‑1 (MQ=60)
                                                                              AATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTT                 >  SRR4302537.2846007/1‑94 (MQ=60)
                                                                              AATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTT                 >  SRR4302537.3511853/1‑94 (MQ=60)
                                                                              AATTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTT                 <  SRR4302537.4002605/94‑1 (MQ=60)
                                                                                TTGCATTTCGCACCCCTCAGATATCAGAAACTGCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCT               <  SRR4302537.749269/94‑1 (MQ=60)
                                                                                TTGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCT               <  SRR4302537.21365/94‑1 (MQ=60)
                                                                                 TGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTT              <  SRR4302537.5717221/94‑1 (MQ=60)
                                                                                 TGCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTT              >  SRR4302537.3770665/1‑94 (MQ=60)
                                                                                  GCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTT             >  SRR4302537.5844565/1‑94 (MQ=60)
                                                                                  GCATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTT             <  SRR4302537.6265961/94‑1 (MQ=60)
                                                                                   CATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTC            >  SRR4302537.3707222/1‑94 (MQ=60)
                                                                                   CATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTC            <  SRR4302537.2880702/94‑1 (MQ=60)
                                                                                   CATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTC            >  SRR4302537.691363/1‑94 (MQ=60)
                                                                                    ATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCT           >  SRR4302537.3418633/1‑94 (MQ=60)
                                                                                    ATTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCT           <  SRR4302537.2741980/94‑1 (MQ=60)
                                                                                     TTTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTT          <  SRR4302537.5020897/94‑1 (MQ=60)
                                                                                      TTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTT         <  SRR4302537.5607694/94‑1 (MQ=60)
                                                                                      TTCGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTT         <  SRR4302537.2922923/94‑1 (MQ=60)
                                                                                        CGCACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAG       <  SRR4302537.4002467/94‑1 (MQ=60)
                                                                                          CACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCA     >  SRR4302537.3277131/1‑94 (MQ=60)
                                                                                          CACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCA     >  SRR4302537.4972078/1‑94 (MQ=60)
                                                                                          CACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCA     <  SRR4302537.5298367/94‑1 (MQ=60)
                                                                                           ACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCAC    >  SRR4302537.4280216/1‑94 (MQ=60)
                                                                                           ACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCAC    <  SRR4302537.5858505/94‑1 (MQ=60)
                                                                                           ACCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCAC    <  SRR4302537.2744389/94‑1 (MQ=60)
                                                                                            CTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACG   <  SRR4302537.160780/94‑1 (MQ=60)
                                                                                            CCCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACG   >  SRR4302537.1614964/1‑94 (MQ=60)
                                                                                             CCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGC  >  SRR4302537.1932057/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTAAACGTAGCATGGTTATTGACGCTTACCTCTACCTCTTTTTCGGTCAGGATCACCACGCAAGGTTCACTGCTGCTCAATTGCATTTCGCACTCCTCAGATATCAGAAACTCCGCTCAAAGGATCTATGCTTCCTGCATGAGTGATCGGCCCGTTCGCCGATAACGATCTTCTTTCTTTAGCACGC  >  NC_000913/586046‑586232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑