Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 630,951 C→T A612V (GCC→GTC)  cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913630,9510CT94.2% 673.9 / 18.6 205A612V (GCC→GTC) cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base C (6/6);  new base T (92/101);  total (98/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-02

Only 100 of 211 total aligned reads displayed.

CGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACA  >  NC_000913/630861‑631040
                                                                                          |                                                                                         
 gcTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCAt                                                                                        <  1:3583220/93‑1 (MQ=255)
  cTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCAtt                                                                                       <  2:569823/93‑1 (MQ=255)
  cTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCAtt                                                                                       <  1:3823246/93‑1 (MQ=255)
   tGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGTCCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGGTGGCTTCCTGGTCAttc                                                                                      >  2:423881/1‑92 (MQ=255)
     gTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCt                                                                                    >  1:1837954/1‑93 (MQ=255)
      tACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCta                                                                                   >  2:2865690/1‑93 (MQ=255)
       aCCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGCAAGCGTTTAGCCCGGCTGCGAAAGTCGGCTTCCTGGTCATTGCtaa                                                                                  >  1:5835183/1‑93 (MQ=255)
       aCCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCtaa                                                                                  <  2:6399664/93‑1 (MQ=255)
       aCCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCtaa                                                                                  <  2:1636940/93‑1 (MQ=255)
       aCCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCtaa                                                                                  <  1:3735390/93‑1 (MQ=255)
          aaCGGCCTGGCTGCTGATTTGTACACTGACCGCAGGCTGGCAGAACGCGTTTAGCCCCCATGCGAAAGTCCGCTTCCTTGGCCTTGCtaataa                                                                               >  2:2633694/1‑93 (MQ=255)
            cGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGt                                                                             <  2:1690125/93‑1 (MQ=255)
             ggCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGtt                                                                            <  1:1945724/93‑1 (MQ=255)
              gCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTc                                                                           >  1:5600339/1‑93 (MQ=255)
              gCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTc                                                                           >  1:1533043/1‑93 (MQ=255)
               ccTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTcc                                                                          >  2:3411467/1‑93 (MQ=255)
               ccTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTcc                                                                          <  2:3987652/93‑1 (MQ=255)
                cTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCa                                                                         <  1:2844328/93‑1 (MQ=255)
                cTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCa                                                                         >  2:442597/1‑93 (MQ=255)
                    ctgctgATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCa                                                                     <  1:2914649/93‑1 (MQ=255)
                     tgctgATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCaa                                                                    >  1:374611/1‑93 (MQ=255)
                      gctgATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAAt                                                                   >  2:3363051/1‑93 (MQ=255)
                      gctgATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAAt                                                                   >  2:297601/1‑93 (MQ=255)
                      gctgATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAAt                                                                   >  2:2495613/1‑93 (MQ=255)
                        tgATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGa                                                                 <  1:760015/93‑1 (MQ=255)
                        tgATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGa                                                                 <  1:4174808/93‑1 (MQ=255)
                         gATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGAt                                                                <  1:4777311/93‑1 (MQ=255)
                          aTTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATc                                                               >  1:2552914/1‑93 (MQ=255)
                          aTTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATc                                                               >  1:917970/1‑93 (MQ=255)
                            ttGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGCCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGa                                                             <  1:3455013/93‑1 (MQ=255)
                              gTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACa                                                           <  1:1460313/93‑1 (MQ=255)
                               tACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAg                                                          <  1:1257161/93‑1 (MQ=255)
                                aCCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGc                                                         <  2:4687576/93‑1 (MQ=255)
                                aCCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGc                                                         <  2:5043129/93‑1 (MQ=255)
                                 cccTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCg                                                        >  1:5654386/1‑93 (MQ=255)
                                  ccTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCgg                                                       >  1:451357/1‑93 (MQ=255)
                                    tGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCa                                                     >  2:5324154/1‑93 (MQ=255)
                                     gACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCaa                                                    <  2:1027790/93‑1 (MQ=255)
                                     gACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCaa                                                    <  1:515001/93‑1 (MQ=255)
                                     gACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCaa                                                    >  2:5164939/1‑93 (MQ=255)
                                      aCCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAat                                                   >  1:4823813/1‑93 (MQ=255)
                                       ccGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAata                                                  >  1:4757605/1‑93 (MQ=255)
                                         gCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGGTCCAGGCAATGATCGACAGCGGCAATGtt                                                >  2:2239777/1‑93 (MQ=255)
                                          cAGGCTGGCAGAAAGCGTTTAGCTCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGGCAGCGGCAATATTc                                               <  2:844040/93‑1 (MQ=255)
                                          cAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTc                                               <  2:6226871/93‑1 (MQ=255)
                                          cAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTc                                               <  1:2400318/93‑1 (MQ=255)
                                           aGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTcc                                              <  2:1923763/93‑1 (MQ=255)
                                            ggCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCg                                             >  2:1973644/1‑93 (MQ=255)
                                             gCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGt                                            <  1:4999992/93‑1 (MQ=255)
                                              cTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTc                                           <  2:4351828/93‑1 (MQ=255)
                                              cTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTc                                           <  2:1005247/93‑1 (MQ=255)
                                              cTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTc                                           >  1:5571068/1‑93 (MQ=255)
                                                    gAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGta                                     >  2:5010952/1‑93 (MQ=255)
                                                     aaaGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGtat                                    <  2:615362/93‑1 (MQ=255)
                                                      aaGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGtata                                   <  1:3154881/93‑1 (MQ=255)
                                                      aaGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGtata                                   >  1:3234631/1‑93 (MQ=255)
                                                      aaGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGtata                                   >  1:2646685/1‑93 (MQ=255)
                                                       aGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATAc                                  >  2:1150364/1‑93 (MQ=255)
                                                        gCGTTTAGGCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACt                                 <  1:4889407/93‑1 (MQ=255)
                                                        gCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACt                                 <  2:5482446/93‑1 (MQ=255)
                                                          gTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGa                               >  2:6113577/1‑93 (MQ=255)
                                                          gTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGa                               >  2:2335017/1‑93 (MQ=255)
                                                          gTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGa                               <  2:4567520/93‑1 (MQ=255)
                                                          gTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGa                               >  1:2594159/1‑93 (MQ=255)
                                                           tttAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAg                              <  1:150580/93‑1 (MQ=255)
                                                           tttAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAg                              <  2:126007/93‑1 (MQ=255)
                                                             tAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTc                            >  2:2825855/1‑93 (MQ=255)
                                                               gCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTcac                          <  1:589441/93‑1 (MQ=255)
                                                                cccGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTcaca                         >  2:5846182/1‑93 (MQ=255)
                                                                  cGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGc                       <  1:1231246/93‑1 (MQ=255)
                                                                  cGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGc                       <  2:353023/93‑1 (MQ=255)
                                                                  cGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGc                       >  1:2338774/1‑93 (MQ=255)
                                                                   ggATGCGACAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCt                      >  2:6315275/1‑93 (MQ=255)
                                                                   ggATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCt                      >  2:4832673/1‑93 (MQ=255)
                                                                   ggATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCt                      >  1:3987505/1‑93 (MQ=255)
                                                                   ggATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCAGTCGCAGTATACTGAGTCACAGCt                      >  2:4878740/1‑93 (MQ=255)
                                                                     aTGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTgg                    >  2:2672728/1‑93 (MQ=255)
                                                                      tGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGgc                   >  1:1193897/1‑93 (MQ=255)
                                                                      tGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGgc                   >  1:5339206/1‑93 (MQ=255)
                                                                       gCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGgcg                  >  1:1333003/1‑93 (MQ=255)
                                                                         gAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCa                >  1:6461423/1‑93 (MQ=255)
                                                                         gAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCa                >  2:3849658/1‑93 (MQ=255)
                                                                         gAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCa                >  2:3448126/1‑93 (MQ=255)
                                                                          aaaGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCaa               <  2:5534810/93‑1 (MQ=255)
                                                                             gTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTg            <  1:4715513/93‑1 (MQ=255)
                                                                              tCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTgg           <  1:4321269/93‑1 (MQ=255)
                                                                              tCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTgg           <  1:4347454/93‑1 (MQ=255)
                                                                                 gCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGTAACTGgtgt        >  2:1747277/1‑93 (MQ=255)
                                                                                 gCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGgtgt        >  1:5049194/1‑93 (MQ=255)
                                                                                 gCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGgtgt        <  1:5460101/93‑1 (MQ=255)
                                                                                 gCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGgtgt        >  1:4652186/1‑93 (MQ=255)
                                                                                 gCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGgtgt        <  1:6279735/93‑1 (MQ=255)
                                                                                 gCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGgtgt        >  2:6020136/1‑93 (MQ=255)
                                                                                 gCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGgtgt        <  2:4389507/93‑1 (MQ=255)
                                                                                 gCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGgtgt        <  2:1406015/93‑1 (MQ=255)
                                                                                  cTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGtt       >  2:3072970/1‑93 (MQ=255)
                                                                                   ttCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGTGCAACTGGTGTTc      <  2:6357958/93‑1 (MQ=255)
                                                                                     ccTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTcaa    <  2:1200357/93‑1 (MQ=255)
                                                                                      cTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTcaac   <  1:816738/93‑1 (MQ=255)
                                                                                       tGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTcaaca  >  1:1005924/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACA  >  NC_000913/630861‑631040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACC  >  NC_000913/630858‑631043
                                                                                             |                                                                                            
TGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGT                                                                                              <  SRR4302537.4800042/94‑1 (MQ=60)
 GGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTC                                                                                             >  SRR4302537.3227208/1‑94 (MQ=60)
   CGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCAT                                                                                           <  SRR4302537.2227516/94‑1 (MQ=60)
   CGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGGCAT                                                                                           >  SRR4302537.3222100/1‑94 (MQ=60)
    GCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATT                                                                                          <  SRR4302537.3608476/94‑1 (MQ=60)
     CTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTG                                                                                         <  SRR4302537.6444877/94‑1 (MQ=60)
     CTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTG                                                                                         <  SRR4302537.3850051/94‑1 (MQ=60)
      TGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGC                                                                                        <  SRR4302537.3392913/94‑1 (MQ=60)
       GGTACCAACGGCCTGGCTGCTGATTTGTACCGTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCT                                                                                       <  SRR4302537.5649669/94‑1 (MQ=60)
       GGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCT                                                                                       >  SRR4302537.2857630/1‑94 (MQ=60)
       GGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCT                                                                                       >  SRR4302537.1850746/1‑94 (MQ=60)
       GGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCT                                                                                       >  SRR4302537.2882647/1‑94 (MQ=60)
        GTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTA                                                                                      <  SRR4302537.640230/94‑1 (MQ=60)
         TACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGCAAGCGTTTAGCCCGGCTGCGAAAGTCGGCTTCCTGGTCATTGCTAA                                                                                     >  SRR4302537.5875176/1‑94 (MQ=60)
          ACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAAT                                                                                    <  SRR4302537.3761627/94‑1 (MQ=60)
            CAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTTATAA                                                                                  >  SRR4302537.2347644/1‑94 (MQ=60)
             AACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCAGGCAGAAAGCGTTTAGCTCGGATTCGAAAGTCGGCTTCCTGGTCATTGCTAATAAG                                                                                 <  SRR4302537.2710394/94‑1 (MQ=60)
              ACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGT                                                                                <  SRR4302537.5555952/94‑1 (MQ=60)
              ACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGT                                                                                <  SRR4302537.4895554/94‑1 (MQ=60)
                GGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTC                                                                              <  SRR4302537.1959252/94‑1 (MQ=60)
                GGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTC                                                                              >  SRR4302537.239717/1‑94 (MQ=60)
                GGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTC                                                                              <  SRR4302537.1559091/94‑1 (MQ=60)
                GGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTC                                                                              >  SRR4302537.1543754/1‑94 (MQ=60)
                GGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTC                                                                              >  SRR4302537.5638625/1‑94 (MQ=60)
                   CTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAG                                                                           <  SRR4302537.5632622/94‑1 (MQ=60)
                   CTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAG                                                                           <  SRR4302537.5712216/94‑1 (MQ=60)
                   CTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAG                                                                           <  SRR4302537.2864235/94‑1 (MQ=60)
                    TGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGG                                                                          <  SRR4302537.4630228/94‑1 (MQ=60)
                     GGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGC                                                                         <  SRR4302537.931106/94‑1 (MQ=60)
                       CTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAA                                                                       >  SRR4302537.2569664/1‑94 (MQ=60)
                       CTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAA                                                                       >  SRR4302537.377209/1‑94 (MQ=60)
                       CTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAA                                                                       <  SRR4302537.2935121/94‑1 (MQ=60)
                        TGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAAT                                                                      >  SRR4302537.4691587/1‑94 (MQ=60)
                         GCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATG                                                                     >  SRR4302537.5881826/1‑94 (MQ=60)
                           TGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGAT                                                                   >  SRR4302537.1044083/1‑94 (MQ=60)
                           TGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGAT                                                                   <  SRR4302537.4203957/94‑1 (MQ=60)
                           TGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGAT                                                                   <  SRR4302537.765209/94‑1 (MQ=60)
                           TGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGAT                                                                   <  SRR4302537.1887718/94‑1 (MQ=60)
                            GATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATC                                                                  <  SRR4302537.4810293/94‑1 (MQ=60)
                            GATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATC                                                                  >  SRR4302537.899101/1‑94 (MQ=60)
                            GATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATC                                                                  <  SRR4302537.1434659/94‑1 (MQ=60)
                            GATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATC                                                                  >  SRR4302537.2570821/1‑94 (MQ=60)
                            GATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATC                                                                  <  SRR4302537.2603025/94‑1 (MQ=60)
                            GATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATC                                                                  >  SRR4302537.924308/1‑94 (MQ=60)
                             ATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCG                                                                 >  SRR4302537.4308789/1‑94 (MQ=60)
                               TTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGCCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGAC                                                               <  SRR4302537.3479451/94‑1 (MQ=60)
                                 GTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAG                                                             <  SRR4302537.723023/94‑1 (MQ=60)
                                 GTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAG                                                             <  SRR4302537.1470475/94‑1 (MQ=60)
                                 GTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAG                                                             >  SRR4302537.4482787/1‑94 (MQ=60)
                                  TACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGC                                                            <  SRR4302537.4510589/94‑1 (MQ=60)
                                  TACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGC                                                            <  SRR4302537.1265909/94‑1 (MQ=60)
                                   ACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCG                                                           >  SRR4302537.5693082/1‑94 (MQ=60)
                                   ACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCG                                                           >  SRR4302537.1501777/1‑94 (MQ=60)
                                    CCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGG                                                          >  SRR4302537.454482/1‑94 (MQ=60)
                                        GACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAAT                                                      <  SRR4302537.518543/94‑1 (MQ=60)
                                        GACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAAT                                                      <  SRR4302537.5082738/94‑1 (MQ=60)
                                        GACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAAT                                                      >  SRR4302537.4857132/1‑94 (MQ=60)
                                         ACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATA                                                     >  SRR4302537.3592505/1‑94 (MQ=60)
                                         ACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATA                                                     >  SRR4302537.4790466/1‑94 (MQ=60)
                                            GCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTC                                                  <  SRR4302537.1300493/94‑1 (MQ=60)
                                             CAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATAGACAGCGGCAATATTCC                                                 >  SRR4302537.5231921/1‑94 (MQ=60)
                                             CAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCC                                                 <  SRR4302537.2417224/94‑1 (MQ=60)
                                              AGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCG                                                <  SRR4302537.4821611/94‑1 (MQ=60)
                                              AGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCG                                                <  SRR4302537.803129/94‑1 (MQ=60)
                                               GGCTGGCAGAAAGCGTTTAGCCCGGATTCAAACGTCGGCTTCCTGGTCATTTCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGT                                               <  SRR4302537.5427152/94‑1 (MQ=60)
                                                GCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTC                                              >  SRR4302537.5609149/1‑94 (MQ=60)
                                                GCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTC                                              <  SRR4302537.4352725/94‑1 (MQ=60)
                                                GCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTC                                              >  SRR4302537.3824768/1‑94 (MQ=60)
                                                GCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTC                                              <  SRR4302537.5034441/94‑1 (MQ=60)
                                                 CTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCG                                             >  SRR4302537.4127367/1‑94 (MQ=60)
                                                 CTGGCAGAAAGCGGTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCG                                             >  SRR4302537.3885397/1‑94 (MQ=60)
                                                  TGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGC                                            <  SRR4302537.2650376/94‑1 (MQ=60)
                                                   GGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCA                                           >  SRR4302537.5001510/1‑94 (MQ=60)
                                                        AAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATA                                      >  SRR4302537.3257430/1‑94 (MQ=60)
                                                        AAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATA                                      >  SRR4302537.2665195/1‑94 (MQ=60)
                                                         AAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATAC                                     <  SRR4302537.3177029/94‑1 (MQ=60)
                                                         AAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATAC                                     >  SRR4302537.1540255/1‑94 (MQ=60)
                                                           GCGTTTAGGCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTG                                   <  SRR4302537.4923173/94‑1 (MQ=60)
                                                           GCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTG                                   <  SRR4302537.5128793/94‑1 (MQ=60)
                                                            CGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGA                                  >  SRR4302537.2612306/1‑94 (MQ=60)
                                                              TTTAGCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGT                                <  SRR4302537.151656/94‑1 (MQ=60)
                                                                  GCCCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACA                            <  SRR4302537.593466/94‑1 (MQ=60)
                                                                   CCCTGATGCAAAAGTTGGATTCCTGGTCATTGCTAATAAGTTCCAGGAAATGAGAGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAG                           <  SRR4302537.1251182/94‑1 (MQ=60)
                                                                    CCGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGC                          <  SRR4302537.610307/94‑1 (MQ=60)
                                                                    CCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGC                          >  SRR4302537.2355260/1‑94 (MQ=60)
                                                                     CGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCT                         <  SRR4302537.1239807/94‑1 (MQ=60)
                                                                     CGGATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCT                         >  SRR4302537.4015433/1‑94 (MQ=60)
                                                                        ATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGC                      >  SRR4302537.5375805/1‑94 (MQ=60)
                                                                        ATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGC                      >  SRR4302537.3615747/1‑94 (MQ=60)
                                                                        ATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGC                      <  SRR4302537.4802287/94‑1 (MQ=60)
                                                                        ATGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGC                      >  SRR4302537.1202186/1‑94 (MQ=60)
                                                                         TGCGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCG                     >  SRR4302537.1342288/1‑94 (MQ=60)
                                                                           CGAAAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCA                   >  SRR4302537.6505208/1‑94 (MQ=60)
                                                                              AAGTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACT                <  SRR4302537.4767987/94‑1 (MQ=60)
                                                                                GTCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGG              <  SRR4302537.4748126/94‑1 (MQ=60)
                                                                                 TCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGT             <  SRR4302537.4351308/94‑1 (MQ=60)
                                                                                 TCGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGT             <  SRR4302537.4377586/94‑1 (MQ=60)
                                                                                  CGGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCTGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTG            <  SRR4302537.3764143/94‑1 (MQ=60)
                                                                                   GGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGT           >  SRR4302537.4684351/1‑94 (MQ=60)
                                                                                   GGCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGT           >  SRR4302537.5083959/1‑94 (MQ=60)
                                                                                    GCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTT          <  SRR4302537.5497347/94‑1 (MQ=60)
                                                                                    GCTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTT          <  SRR4302537.6322805/94‑1 (MQ=60)
                                                                                    GCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTT          >  SRR4302537.5087552/1‑94 (MQ=60)
                                                                                     CTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTC         <  SRR4302537.3089639/94‑1 (MQ=60)
                                                                                     CTTCCTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTC         >  SRR4302537.1212488/1‑94 (MQ=60)
                                                                                         CTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACA     <  SRR4302537.822350/94‑1 (MQ=60)
                                                                                         CTGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACA     >  SRR4302537.1012867/1‑94 (MQ=60)
                                                                                         CTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACA     <  SRR4302537.3451138/94‑1 (MQ=60)
                                                                                          TGGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAA    >  SRR4302537.3058160/1‑94 (MQ=60)
                                                                                           GGTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAAC   <  SRR4302537.3282898/94‑1 (MQ=60)
                                                                                            GTCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACC  <  SRR4302537.3902155/94‑1 (MQ=60)
                                                                                             |                                                                                            
TGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACC  >  NC_000913/630858‑631043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑