Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	686,978	T→C	intergenic (‑8/+242)	ybeJ ← / ← trs53	putative periplasmic binding transport protein/IS5 transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	686,978	0	T	C	96.8%	718.9 / 6.5	221	intergenic (‑8/+242)	ybeJ/trs53	putative periplasmic binding transport protein/IS5 transposase
Reads supporting (aligned to +/- strand): ref base T (4/3); new base C (88/126); total (92/129)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 232 total aligned reads displayed.

GCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCC  >  NC_000913/686888‑687067

gCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAAcccc                                                                                         <  1:3138433/93‑1 (MQ=255)

gCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAAcccc                                                                                         <  2:3565999/93‑1 (MQ=255)

  cGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCCACTCCtt                                                                                       >  2:6009925/1‑93 (MQ=255)

   ggggCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGGTTACGTAATTGCATATCCAACCCCttt                                                                                      >  1:4893972/1‑93 (MQ=255)

    gggCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTa                                                                                     <  1:3104799/93‑1 (MQ=255)

    gggCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTa                                                                                     <  1:2912001/93‑1 (MQ=255)

    gggCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTa                                                                                     <  2:2900629/93‑1 (MQ=255)

     ggcggcGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTAt                                                                                    >  1:6411424/1‑93 (MQ=255)

      gcggcgTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTAtc                                                                                   <  2:3020021/93‑1 (MQ=255)

      gcggcgTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTAtc                                                                                   <  2:714361/93‑1 (MQ=255)

      gcggcgTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGGTTACGTAATTGCATATTCAACCCCTTTAtc                                                                                   >  2:5869754/1‑93 (MQ=255)

         gcgTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTAtcttc                                                                                >  1:2497823/1‑93 (MQ=255)

         gcgTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGGTTACGTAATTGCATATCCCAACCCTTTAtcttc                                                                                >  1:5540531/1‑93 (MQ=255)

          ggTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTAtcttct                                                                               <  2:3791374/92‑1 (MQ=255)

          cgTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTAtcttct                                                                               <  2:4835266/93‑1 (MQ=255)

            tCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTTTCTTCTGc                                                                             >  1:2338508/1‑93 (MQ=255)

            tCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGc                                                                             >  2:410457/1‑93 (MQ=255)

            tCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGc                                                                             <  1:786746/93‑1 (MQ=255)

              aTCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGt                                                                           >  2:6399398/1‑93 (MQ=255)

              aTCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGt                                                                           <  1:5608490/93‑1 (MQ=255)

               tCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGtt                                                                          >  1:5201770/1‑93 (MQ=255)

                 tGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTaa                                                                        <  1:6261336/93‑1 (MQ=255)

                   ccTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGa                                                                      <  2:1925979/93‑1 (MQ=255)

                    cTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGaa                                                                     <  1:4577547/93‑1 (MQ=255)

                    cTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGaa                                                                     <  2:2191406/93‑1 (MQ=255)

                    cTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGaa                                                                     >  1:3723961/1‑93 (MQ=255)

                    cTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGaa                                                                     >  1:4819174/1‑93 (MQ=255)

                     tgtgCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAAc                                                                    <  1:3165345/93‑1 (MQ=255)

                     tgtgCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGGTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAAc                                                                    >  2:5295723/1‑93 (MQ=255)

                      tttccAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACg                                                                   <  2:4514711/90‑1 (MQ=255)

                        gCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCa                                                                 <  2:168517/93‑1 (MQ=255)

                          cAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACGCCTTTATCTTCTGCGTTAAGAACGCATg                                                               >  1:929373/1‑93 (MQ=255)

                          cAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATg                                                               <  2:2845511/93‑1 (MQ=255)

                            gTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGa                                                             <  1:1143037/93‑1 (MQ=255)

                            gTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGGAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGa                                                             >  1:1882613/1‑93 (MQ=255)

                              ccTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATa                                                           >  2:1956705/1‑93 (MQ=255)

                                   ggAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGt                                                      <  2:2310803/93‑1 (MQ=255)

                                   ggAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGt                                                      <  2:3902602/93‑1 (MQ=255)

                                    gAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGtt                                                     <  2:141614/93‑1 (MQ=255)

                                     aaaGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTtg                                                    >  1:4141361/1‑93 (MQ=255)

                                      aaGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTtgt                                                   >  1:5169801/1‑93 (MQ=255)

                                           ccAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAgtg                                              >  1:1841798/1‑93 (MQ=255)

                                            cAGGGCGAGGATTGCTGTGGCAGGTTTCCGTCATTGAAAATACAACCCCCTTATCTTCCGCGTTAAGAACGCATGGATAACCGTTGTGAgtgt                                             >  2:5251416/1‑93 (MQ=255)

                                               ggCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTtg                                          <  1:5913916/93‑1 (MQ=255)

                                               ggCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTtg                                          <  1:4436242/93‑1 (MQ=255)

                                                 cGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTCTTtgtg                                        <  2:3733250/93‑1 (MQ=255)

                                                  gAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTgtgt                                       <  1:6337117/93‑1 (MQ=255)

                                                  gAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTgtgt                                       <  2:6159730/93‑1 (MQ=255)

                                                   aGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGtgtt                                      <  2:4957408/93‑1 (MQ=255)

                                                   aGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGtgtt                                      >  2:4898267/1‑93 (MQ=255)

                                                   aGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGtgtt                                      <  1:2044243/93‑1 (MQ=255)

                                                    ggATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGtgttg                                     >  1:3923372/1‑93 (MQ=255)

                                                     gATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGtgttgt                                    <  2:4359372/93‑1 (MQ=255)

                                                      aTTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTgttgtt                                   <  1:5304351/93‑1 (MQ=255)

                                                       ttGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTa                                  <  2:5436425/93‑1 (MQ=255)

                                                       ttGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTa                                  >  2:1126493/1‑93 (MQ=255)

                                                       ttGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTa                                  <  1:4265729/93‑1 (MQ=255)

                                                        tGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTAt                                 <  2:19838/93‑1 (MQ=255)

                                                         gCTGTGTCTAGTTTACGTAGTTGCATATCTACTTCCTTGACCTGCTGCGTTAAGAACGCATGGACACACGTTGTGAGTGTTTGTGTTGTTATc                                <  2:2741965/93‑1 (MQ=255)

                                                         gCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATc                                <  1:3802265/93‑1 (MQ=255)

                                                          cTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCg                               <  1:4583303/93‑1 (MQ=255)

                                                          cTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCg                               >  1:3863421/1‑93 (MQ=255)

                                                          cTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCg                               >  1:2465490/1‑93 (MQ=255)

                                                          cTGTGGCAGGTTTACGTAATTGCATAT‑CAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGt                              <  1:4700993/93‑1 (MQ=255)

                                                           tgtgGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGt                              <  2:907639/93‑1 (MQ=255)

                                                            gtgGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTc                             <  2:5362228/93‑1 (MQ=255)

                                                            gtgGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTc                             <  1:4064236/93‑1 (MQ=255)

                                                              ggCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTg                           >  2:46487/1‑93 (MQ=255)

                                                               gCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGc                          >  1:3548319/1‑93 (MQ=255)

                                                               gCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGc                          >  1:5997544/1‑93 (MQ=255)

                                                               gCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGc                          >  1:133596/1‑93 (MQ=255)

                                                                 aGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCaa                        <  1:2731117/93‑1 (MQ=255)

                                                                 aGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCaa                        >  1:982917/1‑93 (MQ=255)

                                                                  ggTTTACGTAGTTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAAc                       >  2:3948583/1‑93 (MQ=255)

                                                                  ggTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAAc                       >  1:2077336/1‑93 (MQ=255)

                                                                  ggTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAAc                       <  1:5593778/93‑1 (MQ=255)

                                                                  ggTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAAc                       <  1:6203255/93‑1 (MQ=255)

                                                                    tttACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACtt                     <  2:4045563/93‑1 (MQ=255)

                                                                    tttACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACtt                     >  2:1303598/1‑93 (MQ=255)

                                                                     ttACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACttt                    <  2:2820218/93‑1 (MQ=255)

                                                                     ttACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACttt                    <  1:1005301/93‑1 (MQ=255)

                                                                       aCGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTAt                  >  1:1734146/1‑93 (MQ=255)

                                                                       aCGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTAt                  <  2:3153708/93‑1 (MQ=255)

                                                                        cGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTAtt                 <  2:865720/93‑1 (MQ=255)

                                                                         gTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATtg                >  1:5824751/1‑93 (MQ=255)

                                                                          tAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATtgt               <  1:2861464/93‑1 (MQ=255)

                                                                          tAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATtgt               >  1:4353761/1‑93 (MQ=255)

                                                                            aTTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGc             <  2:1546957/93‑1 (MQ=255)

                                                                            aTTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGc             >  2:6252862/1‑93 (MQ=255)

                                                                               tcATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgt          <  1:4972002/92‑1 (MQ=255)

                                                                               gCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgt          <  2:5024483/93‑1 (MQ=255)

                                                                               gCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgt          >  2:1893583/1‑93 (MQ=255)

                                                                                cATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgtg         >  1:654336/1‑93 (MQ=255)

                                                                                 atatCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgtgt        >  2:5115395/1‑93 (MQ=255)

                                                                                 atatCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgtgt        >  2:4739574/1‑93 (MQ=255)

                                                                                 atatCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgtgt        <  2:575500/93‑1 (MQ=255)

                                                                                 atatCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgtgt        <  1:2151772/93‑1 (MQ=255)

                                                                                  tatCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGtgtt       >  1:1445260/1‑93 (MQ=255)

                                                                                    tCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGtgttgt     >  2:3348648/1‑93 (MQ=255)

                                                                                      cAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGc   <  2:3507577/93‑1 (MQ=255)

GCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCC  >  NC_000913/686888‑687067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTT  >  NC_000913/686885‑687071

GCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACC                                                                                               <  SRR4302537.2655602/94‑1 (MQ=60)

   GCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCT                                                                                            <  SRR4302537.3160458/94‑1 (MQ=60)

    CCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTT                                                                                           <  SRR4302537.1362524/94‑1 (MQ=60)

    CCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTT                                                                                           <  SRR4302537.215954/94‑1 (MQ=60)

     CGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGGTTACGTAATTGCATATCCAACCCCTTT                                                                                          >  SRR4302537.4927766/1‑94 (MQ=60)

      GGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTATTTGCATATCCAACTCtctta                                                                                         >  SRR4302537.3213917/1‑89 (MQ=60)

      GGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGGTTACGTAATTGCATATCCAACCCCTTTA                                                                                         >  SRR4302537.4898316/1‑94 (MQ=60)

       GGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTAT                                                                                        <  SRR4302537.2190103/94‑1 (MQ=60)

       GGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTAT                                                                                        <  SRR4302537.3126484/94‑1 (MQ=60)

       GGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTAT                                                                                        <  SRR4302537.2932457/94‑1 (MQ=60)

       GGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTAT                                                                                        <  SRR4302537.1732964/94‑1 (MQ=60)

       GGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTAT                                                                                        >  SRR4302537.6455042/1‑94 (MQ=60)

         GCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCT                                                                                      <  SRR4302537.296607/94‑1 (MQ=60)

           GGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTC                                                                                    <  SRR4302537.1208034/94‑1 (MQ=60)

           GGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTC                                                                                    >  SRR4302537.2515403/1‑94 (MQ=60)

           GGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGGTTACGTAATTGCATATCCCAACCCTTTATCTTC                                                                                    >  SRR4302537.5578361/1‑94 (MQ=60)

           GGCGTCATCTGCCTGCGCCAGTCCTGCGGAAAGCGCCAGGGCGATGATTGCTGTGGCAGGTTTTCGTAAATGGATTACCAACCCCTTTATCTTC                                                                                    >  SRR4302537.5654432/1‑94 (MQ=60)

            GCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCT                                                                                   <  SRR4302537.5492707/94‑1 (MQ=60)

             CGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTG                                                                                  <  SRR4302537.2378237/94‑1 (MQ=60)

              GTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTTTCTTCTGC                                                                                 >  SRR4302537.2354994/1‑94 (MQ=60)

               TCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCG                                                                                <  SRR4302537.792149/94‑1 (MQ=60)

                 ATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTT                                                                              <  SRR4302537.5646834/94‑1 (MQ=60)

                 ATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTT                                                                              >  SRR4302537.559060/1‑94 (MQ=60)

                 ATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTT                                                                              >  SRR4302537.5237553/1‑94 (MQ=60)

                 ATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGGTTACGTAATTGCATATCCAACCCCTTTATCTTCTTCGTT                                                                              >  SRR4302537.45731/1‑94 (MQ=60)

                 ATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGGTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTT                                                                              >  SRR4302537.95203/1‑94 (MQ=60)

                    TGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAG                                                                           <  SRR4302537.6304322/94‑1 (MQ=60)

                    TGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAG                                                                           >  SRR4302537.3156420/1‑94 (MQ=60)

                     GCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCCACCCCTTTATCTTCTGCGTTAAGA                                                                          >  SRR4302537.644184/1‑94 (MQ=60)

                     GCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGA                                                                          >  SRR4302537.2066018/1‑94 (MQ=60)

                     GCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGA                                                                          <  SRR4302537.6146013/94‑1 (MQ=60)

                      CCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAA                                                                         >  SRR4302537.4852449/1‑94 (MQ=60)

                      CCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAA                                                                         >  SRR4302537.3750128/1‑94 (MQ=60)

                       CTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAAC                                                                        <  SRR4302537.4609225/94‑1 (MQ=60)

                        TGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACG                                                                       <  SRR4302537.2328383/94‑1 (MQ=60)

                        TGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACG                                                                       <  SRR4302537.3187571/94‑1 (MQ=60)

                        TGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACG                                                                       <  SRR4302537.4264845/94‑1 (MQ=60)

                           GCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCAT                                                                    <  SRR4302537.4366190/94‑1 (MQ=60)

                            CCAGTCCTGCGGAAAGCGCCAGGGCGATGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATG                                                                   <  SRR4302537.415804/94‑1 (MQ=60)

                            CCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACGCCTTTATCTTCTGCGTTAAGAACGCATG                                                                   >  SRR4302537.935778/1‑94 (MQ=60)

                            CCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATG                                                                   <  SRR4302537.5217888/94‑1 (MQ=60)

                            CCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATG                                                                   <  SRR4302537.2560256/94‑1 (MQ=60)

                              AGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGA                                                                 <  SRR4302537.188819/94‑1 (MQ=60)

                              AGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGA                                                                 <  SRR4302537.3491437/94‑1 (MQ=60)

                              AGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGGAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGA                                                                 >  SRR4302537.1895703/1‑94 (MQ=60)

                               GTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGAT                                                                <  SRR4302537.1150984/94‑1 (MQ=60)

                                 CCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATAC                                                              <  SRR4302537.99719/94‑1 (MQ=60)

                                  CTGCGGAAAGCGCCAGGGCGAGGATTGCTGGGGCAGGGTTACGGAATTGCATATCCAACCCCTTTATCTTCTCCGTTAAGGAAGCATTGATACC                                                             >  SRR4302537.402465/1‑94 (MQ=60)

                                      GGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTT                                                         <  SRR4302537.2899285/94‑1 (MQ=60)

                                       GAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTG                                                        >  SRR4302537.4170251/1‑94 (MQ=60)

                                        AAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGT                                                       >  SRR4302537.5205403/1‑94 (MQ=60)

                                            CGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGT                                                   <  SRR4302537.5731114/94‑1 (MQ=60)

                                            CGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGT                                                   <  SRR4302537.1921705/94‑1 (MQ=60)

                                            CGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGT                                                   >  SRR4302537.4120250/1‑94 (MQ=60)

                                             GCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGgggg                                                  >  SRR4302537.510415/1‑90 (MQ=60)

                                             GCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTG                                                  <  SRR4302537.6416785/94‑1 (MQ=60)

                                             GCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTG                                                  >  SRR4302537.6127690/1‑94 (MQ=60)

                                             GCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTG                                                  >  SRR4302537.1854618/1‑94 (MQ=60)

                                              CCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGT                                                 <  SRR4302537.663590/94‑1 (MQ=60)

                                              CCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGT                                                 >  SRR4302537.6477909/1‑94 (MQ=60)

                                               CAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTT                                                >  SRR4302537.1603663/1‑94 (MQ=60)

                                                  GGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGT                                             <  SRR4302537.4466872/94‑1 (MQ=60)

                                                  GGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGT                                             <  SRR4302537.5954505/94‑1 (MQ=60)

                                                   GCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCATCCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTG                                            <  SRR4302537.809645/94‑1 (MQ=60)

                                                    CGAGGATTGCTGTGGCAGGGTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGT                                           >  SRR4302537.2508349/1‑94 (MQ=60)

                                                     GAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTT                                          <  SRR4302537.6380430/94‑1 (MQ=60)

                                                     GAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTT                                          >  SRR4302537.3570833/1‑94 (MQ=60)

                                                      AGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTG                                         <  SRR4302537.2058559/94‑1 (MQ=60)

                                                      AGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTG                                         >  SRR4302537.3950785/1‑94 (MQ=60)

                                                      AGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTG                                         <  SRR4302537.5861532/94‑1 (MQ=60)

                                                       GGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGT                                        <  SRR4302537.204581/94‑1 (MQ=60)

                                                        GATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTT                                       <  SRR4302537.6306242/94‑1 (MQ=60)

                                                         ATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTA                                      <  SRR4302537.5340809/94‑1 (MQ=60)

                                                          TTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTAT                                     <  SRR4302537.4466909/94‑1 (MQ=60)

                                                          TTGCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTAT                                     <  SRR4302537.4295369/94‑1 (MQ=60)

                                                            GCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCG                                   <  SRR4302537.806697/94‑1 (MQ=60)

                                                            GCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCG                                   >  SRR4302537.2482825/1‑94 (MQ=60)

                                                            GCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCG                                   <  SRR4302537.3828936/94‑1 (MQ=60)

                                                            GCTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCG                                   >  SRR4302537.3890465/1‑94 (MQ=60)

                                                             CTGTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGT                                  <  SRR4302537.4615004/94‑1 (MQ=60)

                                                             CTGTGGCAGGTTTACGTAATTGCATAT‑CAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTC                                 <  SRR4302537.4733512/94‑1 (MQ=60)

                                                               GTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCT                                <  SRR4302537.6020494/94‑1 (MQ=60)

                                                               GTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCT                                <  SRR4302537.4092636/94‑1 (MQ=60)

                                                               GTGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCT                                >  SRR4302537.4659348/1‑94 (MQ=60)

                                                                TGGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTG                               <  SRR4302537.4387894/94‑1 (MQ=60)

                                                                 GGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGC                              >  SRR4302537.6038743/1‑94 (MQ=60)

                                                                 GGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGC                              >  SRR4302537.3573386/1‑94 (MQ=60)

                                                                 GGCAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGC                              >  SRR4302537.134542/1‑94 (MQ=60)

                                                                   CAGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAA                            >  SRR4302537.989707/1‑94 (MQ=60)

                                                                    AGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAAC                           <  SRR4302537.6288446/94‑1 (MQ=60)

                                                                    AGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGCGTGTTTGTGTTGTTATCGTCTGCAAC                           >  SRR4302537.4966060/1‑94 (MQ=60)

                                                                    AGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAAC                           >  SRR4302537.4827462/1‑94 (MQ=60)

                                                                    AGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAAC                           <  SRR4302537.2750182/94‑1 (MQ=60)

                                                                    AGGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAAC                           >  SRR4302537.2091965/1‑94 (MQ=60)

                                                                     GGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACT                          <  SRR4302537.6246012/94‑1 (MQ=60)

                                                                     GGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACT                          <  SRR4302537.5632022/94‑1 (MQ=60)

                                                                     GGTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACT                          <  SRR4302537.2964346/94‑1 (MQ=60)

                                                                      GTTTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTAGTGTTGTTATCGTCTGCAACTT                         >  SRR4302537.5239539/1‑94 (MQ=60)

                                                                        TTACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTA                       <  SRR4302537.1012239/94‑1 (MQ=60)

                                                                         TACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTAT                      >  SRR4302537.1746241/1‑94 (MQ=60)

                                                                         TACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTAT                      >  SRR4302537.1831475/1‑94 (MQ=60)

                                                                          ACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATT                     <  SRR4302537.1114866/94‑1 (MQ=60)

                                                                          ACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATT                     >  SRR4302537.4609707/1‑94 (MQ=60)

                                                                          ACGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATT                     >  SRR4302537.317688/1‑94 (MQ=60)

                                                                           CGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTG                    >  SRR4302537.5864668/1‑94 (MQ=60)

                                                                           CGTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAAAGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTG                    >  SRR4302537.2788399/1‑94 (MQ=60)

                                                                            GTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGT                   >  SRR4302537.4383907/1‑94 (MQ=60)

                                                                            GTAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGT                   >  SRR4302537.4806354/1‑94 (MQ=60)

                                                                             TAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTG                  <  SRR4302537.2747976/94‑1 (MQ=60)

                                                                             TAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTG                  <  SRR4302537.2881529/94‑1 (MQ=60)

                                                                             TAATTGCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTG                  <  SRR4302537.3868959/94‑1 (MQ=60)

                                                                                  TCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTG             <  SRR4302537.5006272/94‑1 (MQ=60)

                                                                                  GCATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTG             >  SRR4302537.658792/1‑94 (MQ=60)

                                                                                   CATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGT            >  SRR4302537.5554401/1‑94 (MQ=60)

                                                                                    ATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTT           >  SRR4302537.1455331/1‑94 (MQ=60)

                                                                                    ATATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTT           <  SRR4302537.2166938/94‑1 (MQ=60)

                                                                                     TATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTG          <  SRR4302537.5944474/94‑1 (MQ=60)

                                                                                      ATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGT         >  SRR4302537.3684942/1‑94 (MQ=60)

                                                                                      ATCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGT         <  SRR4302537.5007108/94‑1 (MQ=60)

                                                                                       TCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTG        <  SRR4302537.3851014/94‑1 (MQ=60)

                                                                                       TCCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTG        >  SRR4302537.3923860/1‑94 (MQ=60)

                                                                                        CCAACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGC       >  SRR4302537.5644761/1‑94 (MQ=60)

                                                                                          AACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCT     >  SRR4302537.3212896/1‑94 (MQ=60)

                                                                                           ACCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTG    >  SRR4302537.477805/1‑94 (MQ=60)

                                                                                            CCCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGT   >  SRR4302537.1560299/1‑94 (MQ=60)

                                                                                             CCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTT  >  SRR4302537.409167/1‑94 (MQ=60)

                                                                                             CCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTT  >  SRR4302537.2188552/1‑94 (MQ=60)

                                                                                             CCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTT  >  SRR4302537.4451087/1‑94 (MQ=60)

GCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTT  >  NC_000913/686885‑687071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`