Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 763,538 A→G T46A (ACC→GCC)  sucD → succinyl‑CoA synthetase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913763,5380AG96.2% 404.3 / 3.2 132T46A (ACC→GCC) sucDsuccinyl‑CoA synthetase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base G (80/46);  total (83/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 155 total aligned reads displayed.

TTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCA  >  NC_000913/763448‑763627
                                                                                          |                                                                                         
tttACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATTGTTGGCGGCGTAACCCCAGGTAAAGGCGGcgcc                                                                                         >  2:130469/1‑93 (MQ=255)
tttACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGcgcc                                                                                         <  1:910708/93‑1 (MQ=255)
tttACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGcgcc                                                                                         >  1:5411722/1‑93 (MQ=255)
tttACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGcgcc                                                                                         >  1:2030192/1‑93 (MQ=255)
tttACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGcgcc                                                                                         >  1:3099545/1‑93 (MQ=255)
 ttACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGcgcca                                                                                        >  1:3429378/1‑93 (MQ=255)
   aCCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCgccacc                                                                                      >  2:122184/1‑93 (MQ=255)
   aCCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCgccacc                                                                                      <  1:925884/93‑1 (MQ=255)
   aCCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCaccacc                                                                                      >  1:5816013/1‑93 (MQ=255)
    ccGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGccaccc                                                                                     >  2:480383/1‑93 (MQ=255)
       gTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCcacccacc                                                                                  <  2:3024551/93‑1 (MQ=255)
        tAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCt                                                                                 >  1:1089278/1‑93 (MQ=255)
         aGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTc                                                                                <  1:6379440/93‑1 (MQ=255)
          gCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCg                                                                               >  2:4174696/1‑93 (MQ=255)
          gCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCg                                                                               <  1:5066111/93‑1 (MQ=255)
            cAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGc                                                                             >  1:1710168/1‑93 (MQ=255)
            cAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGc                                                                             >  1:3637059/1‑93 (MQ=255)
             aGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGcc                                                                            <  1:2593360/93‑1 (MQ=255)
                ggACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGc                                                                         >  1:1325882/1‑93 (MQ=255)
                ggACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGc                                                                         >  2:564240/1‑93 (MQ=255)
                 gACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGcc                                                                        >  1:2732881/1‑93 (MQ=255)
                  aCTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCg                                                                       >  1:2567506/1‑93 (MQ=255)
                   cTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCgg                                                                      >  2:402677/1‑93 (MQ=255)
                     ttCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGgtg                                                                    >  2:1579364/1‑93 (MQ=255)
                      tCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGgtgt                                                                   <  1:3808210/93‑1 (MQ=255)
                       ccACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGtt                                                                  >  2:3726883/1‑93 (MQ=255)
                        cACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTc                                                                 <  2:643628/93‑1 (MQ=255)
                         aCTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCa                                                                >  2:4432328/1‑93 (MQ=255)
                          cTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCaa                                                               >  2:2905737/1‑93 (MQ=255)
                           tCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAac                                                              >  1:5500520/1‑93 (MQ=255)
                           tCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGCTCAac                                                              >  1:1528232/1‑93 (MQ=255)
                             aGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAacac                                                            >  2:4889023/1‑93 (MQ=255)
                              gAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACAcc                                                           >  2:5166963/1‑93 (MQ=255)
                               aaCAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACcg                                                          >  1:2694405/1‑93 (MQ=255)
                                 cAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTACCCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACcgtg                                                        <  1:1277244/93‑1 (MQ=255)
                                 cAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACcgtg                                                        >  2:2478430/1‑93 (MQ=255)
                                 cAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACcgtg                                                        <  1:6328043/93‑1 (MQ=255)
                                 cAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACcgtg                                                        >  2:1190639/1‑93 (MQ=255)
                                  aGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACcgtgc                                                       <  1:215696/93‑1 (MQ=255)
                                   ggCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACcgtgcg                                                      >  2:3332868/1‑93 (MQ=255)
                                   ggCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACcgtgag                                                      <  2:3970298/93‑3 (MQ=255)
                                     tcATTGCAGCCGGGACTAAAATGGTTGGGGCCCTAACCCCAAGTAAAGGCGGCGCCGCCCACCTCGGCCTGCCGGTGTTCAACACcgtgcgtg                                                    <  1:4294453/92‑1 (MQ=255)
                                     ccATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACcgtgcgtg                                                    >  1:1261048/1‑93 (MQ=255)
                                     ccATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACcgtgcgtg                                                    >  2:741605/1‑93 (MQ=255)
                                      cATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGa                                                   >  2:2276570/1‑93 (MQ=255)
                                      cATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGa                                                   <  2:735095/93‑1 (MQ=255)
                                       aTTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGaa                                                  >  2:3648644/1‑93 (MQ=255)
                                       aTTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGGAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGCGCGTGaa                                                  >  2:176681/1‑93 (MQ=255)
                                         tGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGc                                                <  1:2500042/93‑1 (MQ=255)
                                           cATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCg                                              >  1:2581889/1‑93 (MQ=255)
                                            aTACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGt                                             <  1:1462370/93‑1 (MQ=255)
                                             tACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGtt                                            >  2:1215409/1‑93 (MQ=255)
                                              aCGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTtg                                           <  2:911320/93‑1 (MQ=255)
                                              aCGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTtg                                           >  2:3429177/1‑93 (MQ=255)
                                               cGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTtgc                                          >  1:3746407/1‑93 (MQ=255)
                                                   aCTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGcc                                      <  2:6271913/93‑1 (MQ=255)
                                                    cTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCa                                     >  2:5185819/1‑93 (MQ=255)
                                                    cTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCa                                     <  2:5876037/93‑1 (MQ=255)
                                                     tAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCAc                                    <  1:1004463/93‑1 (MQ=255)
                                                      aaaaTGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACt                                   >  2:4993718/1‑93 (MQ=255)
                                                       aaaTGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTg                                  <  2:2361698/93‑1 (MQ=255)
                                                        aaTGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTgg                                 <  2:6217486/93‑1 (MQ=255)
                                                         aTGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGgc                                <  1:4915502/93‑1 (MQ=255)
                                                          tGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGgcg                               >  1:1347007/1‑93 (MQ=255)
                                                           ggTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGgcgc                              >  2:304087/1‑93 (MQ=255)
                                                           ggTGGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGgcgc                              <  2:4446653/93‑1 (MQ=255)
                                                            gTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCt                             >  1:5292646/1‑93 (MQ=255)
                                                            gTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCt                             <  2:6136808/93‑1 (MQ=255)
                                                              tGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTAc                           <  2:3238197/93‑1 (MQ=255)
                                                              tGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTAc                           <  1:5207100/93‑1 (MQ=255)
                                                               ggcggcGTAACCCCAGGTAAAGGCGGCCCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTAcc                          <  2:1875754/93‑1 (MQ=255)
                                                                gcggcgTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCg                         >  2:3572869/1‑93 (MQ=255)
                                                                gcggcgTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCGGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCg                         >  1:3462101/1‑93 (MQ=255)
                                                                  ggcgTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCt                       >  2:4618749/1‑93 (MQ=255)
                                                                   gcgTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCtt                      >  2:5704426/1‑93 (MQ=255)
                                                                    cgTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCGGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTc                     <  1:481921/93‑1 (MQ=255)
                                                                     gTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCt                    >  1:608003/1‑93 (MQ=255)
                                                                     gTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCt                    >  2:1785566/1‑93 (MQ=255)
                                                                        aCCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGtt                 >  1:6184178/1‑93 (MQ=255)
                                                                        aCCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGCGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACAGCTTCTGtt                 >  1:3357242/1‑93 (MQ=255)
                                                                          cccAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTAt               >  1:4672486/1‑93 (MQ=255)
                                                                          cccAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTAt               >  2:3928322/1‑93 (MQ=255)
                                                                            cAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCt             >  1:4160026/1‑93 (MQ=255)
                                                                            cAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCt             >  2:5882614/1‑93 (MQ=255)
                                                                             aGGTAAAGGCGGCGCCCCCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTa            <  1:6128909/93‑1 (MQ=255)
                                                                             aGGTAAAGGCGGCGCCCCCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTa            <  2:6422009/93‑1 (MQ=255)
                                                                             aGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTa            <  2:4067781/93‑1 (MQ=255)
                                                                             aGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTa            >  2:5916283/1‑93 (MQ=255)
                                                                             aGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTa            <  2:4788911/93‑1 (MQ=255)
                                                                             aGGTAAAGGCGCCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTa            <  1:3323769/93‑1 (MQ=255)
                                                                              ggTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTAc           >  1:2203939/1‑93 (MQ=255)
                                                                               gTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACg          >  1:3669792/1‑93 (MQ=255)
                                                                                  aaGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTAc       >  2:4334352/1‑93 (MQ=255)
                                                                                  aaGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTAc       <  2:614665/93‑1 (MQ=255)
                                                                                   aGGCGGCGCCACCCCCCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTAcc      <  2:314404/93‑1 (MQ=255)
                                                                                     gcggcgCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAg    <  2:2961406/93‑1 (MQ=255)
                                                                                     gcggcCCCCCCCACCCCGGCCTGCCGGTTTACAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCTCTTCTGTTATCTACGTACCAg    <  2:1524722/93‑1 (MQ=255)
                                                                                      cggcGCCACCCCCCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGc   <  2:1176468/93‑1 (MQ=255)
                                                                                      cggcGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGc   >  1:1829171/1‑93 (MQ=255)
                                                                                       ggcCCCTCCCGCCTCGGCCTGCCGGTGTCCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCa  <  2:2329448/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCA  >  NC_000913/763448‑763627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCTTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCG  >  NC_000913/763445‑763630
                                                                                             |                                                                                            
GGCTTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCG                                                                                              <  SRR4302537.2085218/94‑1 (MQ=60)
  CTTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCC                                                                                            >  SRR4302537.5448623/1‑94 (MQ=60)
  CTTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCC                                                                                            >  SRR4302537.3627370/1‑94 (MQ=60)
  CTTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCC                                                                                            >  SRR4302537.3121174/1‑94 (MQ=60)
  CTTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCC                                                                                            >  SRR4302537.2044346/1‑94 (MQ=60)
   TTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCA                                                                                           <  SRR4302537.916994/94‑1 (MQ=60)
   TTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCA                                                                                           >  SRR4302537.3453649/1‑94 (MQ=60)
     TACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACC                                                                                         >  SRR4302537.5855880/1‑94 (MQ=60)
      ACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCC                                                                                        <  SRR4302537.932266/94‑1 (MQ=60)
      ACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCC                                                                                        >  SRR4302537.189709/1‑94 (MQ=60)
      ACCGGTAGCCACGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGTGTAACCCCAGGTAAAGGCGGCGCCACCC                                                                                        <  SRR4302537.1581469/94‑1 (MQ=60)
          GTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCT                                                                                    >  SRR4302537.1096802/1‑94 (MQ=60)
            AGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCG                                                                                  <  SRR4302537.6422931/94‑1 (MQ=60)
             GCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGG                                                                                 <  SRR4302537.5100987/94‑1 (MQ=60)
              CCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGC                                                                                >  SRR4302537.1722115/1‑94 (MQ=60)
              CCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGC                                                                                >  SRR4302537.2164188/1‑94 (MQ=60)
              CCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGC                                                                                >  SRR4302537.3662673/1‑94 (MQ=60)
               CAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCC                                                                               >  SRR4302537.411456/1‑94 (MQ=60)
                AGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCT                                                                              >  SRR4302537.1250179/1‑94 (MQ=60)
                AGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCT                                                                              <  SRR4302537.2611502/94‑1 (MQ=60)
                  GGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGC                                                                            >  SRR4302537.1335124/1‑94 (MQ=60)
                   GGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCC                                                                           >  SRR4302537.2751957/1‑94 (MQ=60)
                    GACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCG                                                                          >  SRR4302537.2585496/1‑94 (MQ=60)
                         TCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTT                                                                     <  SRR4302537.3834912/94‑1 (MQ=60)
                            ACTCACAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAA                                                                  <  SRR4302537.6474667/94‑1 (MQ=60)
                             CTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAca                                                                 >  SRR4302537.4520863/1‑92 (MQ=60)
                             CTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAAC                                                                 >  SRR4302537.5538082/1‑94 (MQ=60)
                             CTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGCTCAAC                                                                 >  SRR4302537.1538910/1‑94 (MQ=60)
                                 GAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCG                                                             >  SRR4302537.2713231/1‑94 (MQ=60)
                                  AACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGT                                                            >  SRR4302537.5507625/1‑94 (MQ=60)
                                    CAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTACCCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGC                                                          <  SRR4302537.1286119/94‑1 (MQ=60)
                                    CAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGC                                                          <  SRR4302537.6371320/94‑1 (MQ=60)
                                     AGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCG                                                         >  SRR4302537.4826209/1‑94 (MQ=60)
                                     AGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCG                                                         <  SRR4302537.217237/94‑1 (MQ=60)
                                     AGGACATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCG                                                         <  SRR4302537.3108243/94‑1 (MQ=60)
                                      GGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGT                                                        <  SRR4302537.4988474/94‑1 (MQ=60)
                                       GCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTG                                                       >  SRR4302537.1269820/1‑94 (MQ=60)
                                        tcattgcagccgggACTAAAATGGTTGGGGCCCTAACCCCAAGTAAAGGCGGCGCCGCCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGA                                                      <  SRR4302537.4324311/80‑1 (MQ=60)
                                          ATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAG                                                    <  SRR4302537.532650/94‑1 (MQ=60)
                                            TGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCC                                                  >  SRR4302537.3402098/1‑94 (MQ=60)
                                            TGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCC                                                  <  SRR4302537.2693414/94‑1 (MQ=60)
                                            TGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCC                                                  <  SRR4302537.2517634/94‑1 (MQ=60)
                                            TGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCC                                                  >  SRR4302537.2404817/1‑94 (MQ=60)
                                             GCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCG                                                 >  SRR4302537.2599959/1‑94 (MQ=60)
                                               ATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTT                                               <  SRR4302537.1472542/94‑1 (MQ=60)
                                                TACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTG                                              <  SRR4302537.3763063/94‑1 (MQ=60)
                                                 ACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGC                                             >  SRR4302537.3772721/1‑94 (MQ=60)
                                                     CACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCC                                         <  SRR4302537.627488/94‑1 (MQ=60)
                                                      ACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCA                                        >  SRR4302537.1396729/1‑94 (MQ=60)
                                                       CTAAAATGGTTGGCGGCGTAACCCCAGGGAAACGCGGCGCCACCCACCTCGGCCTGCCGATGTTCAAGACCGTGCGTGAAGCCGTTGCTGCCAC                                       >  SRR4302537.3187581/1‑94 (MQ=60)
                                                        TAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACT                                      <  SRR4302537.1011396/94‑1 (MQ=60)
                                                         AAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTG                                     >  SRR4302537.6161812/1‑94 (MQ=60)
                                                            ATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCG                                  <  SRR4302537.4949423/94‑1 (MQ=60)
                                                            ATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCG                                  >  SRR4302537.1356396/1‑94 (MQ=60)
                                                              GGTTGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCT                                >  SRR4302537.5329019/1‑94 (MQ=60)
                                                                 TGGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACC                             <  SRR4302537.5242916/94‑1 (MQ=60)
                                                                  GGCGGCGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCGGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCG                            >  SRR4302537.3486578/1‑94 (MQ=60)
                                                                       CGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCGGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCT                       <  SRR4302537.485222/94‑1 (MQ=60)
                                                                       CGTAACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCT                       >  SRR4302537.612160/1‑94 (MQ=60)
                                                                          AACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTT                    >  SRR4302537.5968073/1‑94 (MQ=60)
                                                                          AACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTT                    >  SRR4302537.6226779/1‑94 (MQ=60)
                                                                          AACCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGCGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACAGCTTCTGTT                    >  SRR4302537.3381022/1‑94 (MQ=60)
                                                                           ACCCCCGGCAAGGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACtgtcaaggaagccgtggaagccaccggcgccgaagcctcggtca                   <  SRR4302537.6487436/94‑45 (MQ=55)
                                                                            CCCCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTAT                  >  SRR4302537.4704815/1‑94 (MQ=60)
                                                                              CCAGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCT                >  SRR4302537.4189079/1‑94 (MQ=60)
                                                                                AGGTAAAGGCGGCGCCCCCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTAC              <  SRR4302537.6171032/94‑1 (MQ=60)
                                                                                AGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTAC              <  SRR4302537.3137090/94‑1 (MQ=60)
                                                                                AGGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTAC              >  SRR4302537.2219482/1‑94 (MQ=60)
                                                                                AGGTAAAGGCGCCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTAC              <  SRR4302537.3347288/94‑1 (MQ=60)
                                                                                 GGTAAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACG             >  SRR4302537.3695612/1‑94 (MQ=60)
                                                                                    AAAGGCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTAC          >  SRR4302537.2587942/1‑94 (MQ=60)
                                                                                    AAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTAC          <  SRR4302537.131109/94‑1 (MQ=60)
                                                                                        GCGGCGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGC      >  SRR4302537.1841916/1‑94 (MQ=60)
                                                                                            CGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTCTCTACGTACCACCACCG  >  SRR4302537.2481697/1‑94 (MQ=60)
                                                                                            CGCCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCG  >  SRR4302537.3544801/1‑94 (MQ=60)
                                                                                             |                                                                                            
GGCTTTACCGGTAGCCAGGGGACTTTCCACTCAGAACAGGCCATTGCATACGGCACTAAAATGGTTGGCGGCGTAACCCCAGGTAAAGGCGGCACCACCCACCTCGGCCTGCCGGTGTTCAACACCGTGCGTGAAGCCGTTGCTGCCACTGGCGCTACCGCTTCTGTTATCTACGTACCAGCACCG  >  NC_000913/763445‑763630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑