Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 840,672 A→G V28A (GTG→GCG)  b0805 ← putative outer membrane receptor for iron transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913840,6720AG96.8% 711.8 / 8.2 220V28A (GTG→GCG) b0805putative outer membrane receptor for iron transport
Reads supporting (aligned to +/- strand):  ref base A (5/2);  new base G (108/105);  total (113/107)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 234 total aligned reads displayed.

TGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGT  >  NC_000913/840582‑840763
                                                                                          |                                                                                           
tgttgtGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCcgca                                                                                           <  2:552992/93‑1 (MQ=255)
 gttgtGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAg                                                                                          >  2:3814032/1‑93 (MQ=255)
        cgcATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGAt                                                                                   <  2:4994502/93‑1 (MQ=255)
         gcATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATg                                                                                  >  1:855998/1‑93 (MQ=255)
          cATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGc                                                                                 <  1:6438041/93‑1 (MQ=255)
           aTAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGcc                                                                                >  1:451373/1‑93 (MQ=255)
            tAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCa                                                                               >  1:2728424/1‑93 (MQ=255)
             aaaGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCaa                                                                              <  2:5797482/93‑1 (MQ=255)
               aGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATa                                                                            >  1:1167069/1‑93 (MQ=255)
               aGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATa                                                                            >  1:208638/1‑93 (MQ=255)
                 cGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACa                                                                          <  1:4079569/93‑1 (MQ=255)
                  gAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCACTACaa                                                                         >  1:1600205/1‑93 (MQ=255)
                  gAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACaa                                                                         <  2:5135994/93‑1 (MQ=255)
                    aGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCTATACAAAg                                                                       >  2:4049485/1‑93 (MQ=255)
                     ggCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGa                                                                      >  2:6031849/1‑93 (MQ=255)
                     ggCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGa                                                                      >  2:5100784/1‑93 (MQ=255)
                      gCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGAc                                                                     <  2:3162838/93‑1 (MQ=255)
                        gTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCg                                                                   <  1:1815329/93‑1 (MQ=255)
                          tGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGc                                                                 <  1:5563035/93‑1 (MQ=255)
                           gATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCa                                                                >  2:1903045/1‑93 (MQ=255)
                           gATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCa                                                                <  1:3396279/93‑1 (MQ=255)
                               cTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGa                                                            <  2:2943535/93‑1 (MQ=255)
                               cTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGa                                                            >  1:4416012/1‑93 (MQ=255)
                               cTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGa                                                            <  2:4624675/93‑1 (MQ=255)
                                ttCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGaa                                                           <  2:3667292/93‑1 (MQ=255)
                                ttCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGaa                                                           <  1:1423064/93‑1 (MQ=255)
                                 tCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAAc                                                          <  1:3204191/93‑1 (MQ=255)
                                   gACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGt                                                        <  1:1382733/93‑1 (MQ=255)
                                    aCAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTg                                                       >  2:1572642/1‑93 (MQ=255)
                                    aCAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTg                                                       >  2:1035088/1‑93 (MQ=255)
                                    aCAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTg                                                       >  1:5324734/1‑93 (MQ=255)
                                     cAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGa                                                      <  1:3894803/93‑1 (MQ=255)
                                     cAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGa                                                      <  1:3234651/93‑1 (MQ=255)
                                      aaCCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAg                                                     <  1:3532164/93‑1 (MQ=255)
                                      aaCCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAg                                                     >  2:5943515/1‑93 (MQ=255)
                                      aaCCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAg                                                     <  1:5116093/93‑1 (MQ=255)
                                       aCCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGc                                                    <  1:1962685/93‑1 (MQ=255)
                                       aCCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGc                                                    >  1:1332845/1‑93 (MQ=255)
                                       aCCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGAGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGc                                                    >  2:2083544/1‑93 (MQ=255)
                                        ccAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCg                                                   >  2:268783/1‑93 (MQ=255)
                                           gCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAAt                                                <  1:2286793/93‑1 (MQ=255)
                                           gCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAAt                                                >  1:3038884/1‑93 (MQ=255)
                                            cGTGTCATCCGCGTTAGTTTGCCATTCGGCGGCGAGTGACTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATg                                               <  2:2383451/93‑1 (MQ=255)
                                             gtgtCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGa                                              >  1:3630662/1‑93 (MQ=255)
                                              tgtCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGaa                                             >  1:3285166/1‑93 (MQ=255)
                                              tgtCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGaa                                             >  1:1159733/1‑93 (MQ=255)
                                                tCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTTAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAAt                                           <  2:4778486/93‑1 (MQ=255)
                                                tCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAATAACGTGAGCGAATGAAAt                                           <  2:1315982/93‑1 (MQ=255)
                                                tCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAAt                                           >  1:741489/1‑93 (MQ=255)
                                                tCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAAt                                           <  1:4981234/93‑1 (MQ=255)
                                                tCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAAt                                           <  2:3325619/93‑1 (MQ=255)
                                                  aTCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTg                                         >  2:2561652/1‑93 (MQ=255)
                                                  aTCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTg                                         >  2:4212397/1‑93 (MQ=255)
                                                   tCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAAgtgt                                        >  2:1285554/1‑93 (MQ=255)
                                                   tCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGt                                        >  1:4272849/1‑93 (MQ=255)
                                                    ccGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTc                                       >  1:2428515/1‑93 (MQ=255)
                                                      gcgTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTg                                     <  2:3403594/93‑1 (MQ=255)
                                                      gcgTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTg                                     <  1:5679928/93‑1 (MQ=255)
                                                      gcgTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTg                                     <  1:6265725/93‑1 (MQ=255)
                                                        gTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGc                                   <  2:5635581/93‑1 (MQ=255)
                                                        gTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGc                                   <  2:5974072/93‑1 (MQ=255)
                                                         ttAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCa                                  <  2:4015654/93‑1 (MQ=255)
                                                          tAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAg                                 <  2:1862537/93‑1 (MQ=255)
                                                          tAGTATGCCGCTCGGCGGCGAGTGCCTGAGCCTCAGGCGTGATGCCATTACAAAGTCCGGCAAGGAACGTGAGCGAATGAAAATGTCTGGCAg                                 <  2:2733478/93‑1 (MQ=255)
                                                           aGTTTGCCCTTCGTCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAgg                                <  2:1207964/93‑1 (MQ=255)
                                                           aGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGTCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAgg                                <  2:4387766/93‑1 (MQ=255)
                                                           aGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAgg                                >  2:6091713/1‑93 (MQ=255)
                                                            gTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAggg                               >  2:4059492/1‑93 (MQ=255)
                                                            gTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAggg                               >  1:5996672/1‑93 (MQ=255)
                                                             tttGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGa                              <  2:147226/93‑1 (MQ=255)
                                                                gCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAAt                           >  2:5606265/1‑93 (MQ=255)
                                                                 cccTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAAtt                          >  1:6065175/1‑93 (MQ=255)
                                                                  ccTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTg                         <  1:3802116/93‑1 (MQ=255)
                                                                    ttCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCg                       >  1:612694/1‑93 (MQ=255)
                                                                     tCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGa                      <  1:5850074/93‑1 (MQ=255)
                                                                      cggcggCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCATGGAAATTGCGAt                     <  2:2518035/93‑1 (MQ=255)
                                                                       ggcggcGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGAtt                    >  2:2147794/1‑93 (MQ=255)
                                                                         cggcgAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGt                  >  2:3638158/1‑93 (MQ=255)
                                                                         cggcgAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGt                  <  1:4651720/93‑1 (MQ=255)
                                                                         cggcgAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGt                  <  2:4164149/93‑1 (MQ=255)
                                                                          ggcgAGTGCCTGAGCGGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGtt                 <  2:89857/93‑1 (MQ=255)
                                                                          ggcgAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGtt                 >  1:4764582/1‑93 (MQ=255)
                                                                          ggcgAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGtt                 >  1:1008163/1‑93 (MQ=255)
                                                                           gcgAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGTCCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGttt                <  1:3143690/93‑1 (MQ=255)
                                                                           gcgAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGttt                <  2:1112392/93‑1 (MQ=255)
                                                                           gcgAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGttt                <  1:985838/93‑1 (MQ=255)
                                                                           gcgAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGttt                >  2:6055160/1‑93 (MQ=255)
                                                                           gcgAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGttt                <  1:2938653/93‑1 (MQ=255)
                                                                            cgAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGtttt               >  1:5417687/1‑93 (MQ=255)
                                                                            cgAGTGCCTGAGCCGCAGGCGAGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGtttt               >  2:4107982/1‑93 (MQ=255)
                                                                               gTGCCTGAGCCGCAGGAGTGATGCGAATACAAAGACCGGCAAAGAACGTGAGCGAATGCAATTGTCTGGCAGGGAAATTTCGATTGTTTTCCa            >  2:2901975/1‑93 (MQ=255)
                                                                                 gCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCAtt          >  1:5918983/1‑93 (MQ=255)
                                                                                 gCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCAtt          <  1:4151162/93‑1 (MQ=255)
                                                                                   cTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCAtttt        <  1:4205555/93‑1 (MQ=255)
                                                                                   cTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCAtttt        <  1:273714/93‑1 (MQ=255)
                                                                                   cTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCAtttt        <  2:2163550/93‑1 (MQ=255)
                                                                                    tGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTg       >  2:6423929/1‑93 (MQ=255)
                                                                                      aGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCa     >  1:2654383/1‑93 (MQ=255)
                                                                                      aGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCa     <  2:166772/93‑1 (MQ=255)
                                                                                       gCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAg    >  1:4847322/1‑93 (MQ=255)
                                                                                          |                                                                                           
TGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGT  >  NC_000913/840582‑840763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGT  >  NC_000913/840579‑840763
                                                                                             |                                                                                           
GATTGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCG                                                                                             >  SRR4302537.5569541/1‑94 (MQ=60)
 ATTGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGC                                                                                            >  SRR4302537.904269/1‑94 (MQ=60)
 ATTGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGC                                                                                            >  SRR4302537.1441620/1‑94 (MQ=60)
   TGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAG                                                                                          >  SRR4302537.4833459/1‑94 (MQ=60)
     TTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGC                                                                                        <  SRR4302537.341320/94‑1 (MQ=60)
         GGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGA                                                                                    <  SRR4302537.72218/94‑1 (MQ=60)
           CGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATG                                                                                  >  SRR4302537.861873/1‑94 (MQ=60)
           CGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATG                                                                                  >  SRR4302537.6338649/1‑94 (MQ=60)
             CATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCC                                                                                <  SRR4302537.6481750/94‑1 (MQ=60)
             CATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCC                                                                                >  SRR4302537.454498/1‑94 (MQ=60)
              ATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCA                                                                               <  SRR4302537.2783849/94‑1 (MQ=60)
              ATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCA                                                                               >  SRR4302537.2747476/1‑94 (MQ=60)
              ATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCA                                                                               >  SRR4302537.5964911/1‑94 (MQ=60)
               TAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAA                                                                              >  SRR4302537.5955262/1‑94 (MQ=60)
                AAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAAT                                                                             >  SRR4302537.2506488/1‑94 (MQ=60)
                 AAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATA                                                                            >  SRR4302537.210138/1‑94 (MQ=60)
                 AAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATA                                                                            >  SRR4302537.1175183/1‑94 (MQ=60)
                    CGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCACTACAA                                                                         >  SRR4302537.1611389/1‑94 (MQ=60)
                    CGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAA                                                                         <  SRR4302537.1076916/94‑1 (MQ=60)
                    CGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAA                                                                         <  SRR4302537.4108050/94‑1 (MQ=60)
                       AGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGA                                                                      >  SRR4302537.6134522/1‑94 (MQ=60)
                        GGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGAC                                                                     >  SRR4302537.4593186/1‑94 (MQ=60)
                        GGCGTGGATGCTTCGACAACCAGCGTGTCAGCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGAC                                                                     <  SRR4302537.1584930/94‑1 (MQ=60)
                           GTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGG                                                                  >  SRR4302537.782942/1‑94 (MQ=60)
                           GTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGG                                                                  <  SRR4302537.1827983/94‑1 (MQ=60)
                           GTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGG                                                                  >  SRR4302537.3244254/1‑94 (MQ=60)
                           GTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGG                                                                  >  SRR4302537.2630117/1‑94 (MQ=60)
                             TGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCA                                                                <  SRR4302537.5601056/94‑1 (MQ=60)
                             TGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCA                                                                >  SRR4302537.6186901/1‑94 (MQ=60)
                             TGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCA                                                                <  SRR4302537.543889/94‑1 (MQ=60)
                              GATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAA                                                               <  SRR4302537.3420336/94‑1 (MQ=60)
                                 GCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGA                                                            <  SRR4302537.4736021/94‑1 (MQ=60)
                                 GCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGA                                                            >  SRR4302537.4446500/1‑94 (MQ=60)
                                   TTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAAC                                                          <  SRR4302537.1432974/94‑1 (MQ=60)
                                    TCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACG                                                         <  SRR4302537.3226715/94‑1 (MQ=60)
                                     CGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGT                                                        <  SRR4302537.5810787/94‑1 (MQ=60)
                                     CGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGT                                                        <  SRR4302537.3821585/94‑1 (MQ=60)
                                      GACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTG                                                       <  SRR4302537.1392380/94‑1 (MQ=60)
                                      GACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTG                                                       >  SRR4302537.5361273/1‑94 (MQ=60)
                                       ACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGA                                                      >  SRR4302537.2417763/1‑94 (MQ=60)
                                        CAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAG                                                     <  SRR4302537.3257450/94‑1 (MQ=60)
                                        CAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAG                                                     <  SRR4302537.3922029/94‑1 (MQ=60)
                                         AACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGC                                                    >  SRR4302537.1342130/1‑94 (MQ=60)
                                         AACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGC                                                    >  SRR4302537.5951398/1‑94 (MQ=60)
                                         AACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGC                                                    <  SRR4302537.3557126/94‑1 (MQ=60)
                                         AACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGC                                                    <  SRR4302537.5151354/94‑1 (MQ=60)
                                          ACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCG                                                   <  SRR4302537.1976332/94‑1 (MQ=60)
                                             AGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAAT                                                >  SRR4302537.3265327/1‑94 (MQ=60)
                                             AGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAAT                                                >  SRR4302537.4910832/1‑94 (MQ=60)
                                             AGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAAT                                                >  SRR4302537.3060063/1‑94 (MQ=60)
                                             AGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAAT                                                >  SRR4302537.4396417/1‑94 (MQ=60)
                                             AGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAAT                                                >  SRR4302537.5086064/1‑94 (MQ=60)
                                              GCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGTAAAGAACGTGAGCGAATG                                               >  SRR4302537.4399033/1‑94 (MQ=60)
                                              GCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATG                                               >  SRR4302537.905759/1‑94 (MQ=60)
                                              GCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATG                                               <  SRR4302537.2302923/94‑1 (MQ=60)
                                              ACGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATG                                               <  SRR4302537.4263284/94‑1 (MQ=60)
                                               CGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGA                                              >  SRR4302537.3656246/1‑94 (MQ=60)
                                                GTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAA                                             >  SRR4302537.901076/1‑94 (MQ=60)
                                                GTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAA                                             >  SRR4302537.3308370/1‑94 (MQ=60)
                                                GTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAA                                             <  SRR4302537.2071370/94‑1 (MQ=60)
                                                GTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAA                                             >  SRR4302537.1167802/1‑94 (MQ=60)
                                                  GTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAAT                                           >  SRR4302537.746554/1‑94 (MQ=60)
                                                  GTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAAT                                           >  SRR4302537.746093/1‑94 (MQ=60)
                                                   TCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGGCAATACAAAGACCGGCAACGCACGTGAGCGAATGAAATT                                          >  SRR4302537.3954772/1‑94 (MQ=60)
                                                   TCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATT                                          <  SRR4302537.5015569/94‑1 (MQ=60)
                                                     ATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGT                                        >  SRR4302537.4302539/1‑94 (MQ=60)
                                                     ATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGT                                        >  SRR4302537.2033089/1‑94 (MQ=60)
                                                     ATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGAGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAGTGAGATTGT                                        >  SRR4302537.3201166/1‑94 (MQ=60)
                                                      TCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTC                                       >  SRR4302537.2445624/1‑94 (MQ=60)
                                                       CCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCT                                      <  SRR4302537.1862806/94‑1 (MQ=60)
                                                        CGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTG                                     >  SRR4302537.3083701/1‑94 (MQ=60)
                                                         GCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGG                                    <  SRR4302537.3337027/94‑1 (MQ=60)
                                                         GCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGG                                    <  SRR4302537.3180326/94‑1 (MQ=60)
                                                         GCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGG                                    <  SRR4302537.6308728/94‑1 (MQ=60)
                                                         GCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGG                                    <  SRR4302537.5718783/94‑1 (MQ=60)
                                                          CGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGC                                   >  SRR4302537.5687095/1‑94 (MQ=60)
                                                            TTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAG                                 >  SRR4302537.1613796/1‑94 (MQ=60)
                                                             TAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGG                                <  SRR4302537.6117587/94‑1 (MQ=60)
                                                             TAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGG                                <  SRR4302537.1325586/94‑1 (MQ=60)
                                                              AGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGG                               >  SRR4302537.2954122/1‑94 (MQ=60)
                                                              AGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGG                               >  SRR4302537.6037864/1‑94 (MQ=60)
                                                              AGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGG                               >  SRR4302537.3734130/1‑94 (MQ=60)
                                                              AGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGG                               <  SRR4302537.1298210/94‑1 (MQ=60)
                                                               GTTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGA                              >  SRR4302537.4203139/1‑94 (MQ=60)
                                                                TTTGCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAA                             <  SRR4302537.1172212/94‑1 (MQ=60)
                                                                   GCCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATT                          >  SRR4302537.6106844/1‑94 (MQ=60)
                                                                    CCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTG                         >  SRR4302537.3622119/1‑94 (MQ=60)
                                                                    CCCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTG                         >  SRR4302537.6095953/1‑94 (MQ=60)
                                                                     CCTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGC                        <  SRR4302537.3828787/94‑1 (MQ=60)
                                                                      CTTCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCG                       >  SRR4302537.616873/1‑94 (MQ=60)
                                                                        TCGGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGAT                     <  SRR4302537.5890201/94‑1 (MQ=60)
                                                                          GGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTG                   <  SRR4302537.5053543/94‑1 (MQ=60)
                                                                          GGCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTG                   <  SRR4302537.5492561/94‑1 (MQ=60)
                                                                           GCGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGT                  >  SRR4302537.2999606/1‑94 (MQ=60)
                                                                            CGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTT                 >  SRR4302537.4797487/1‑94 (MQ=60)
                                                                            CGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTT                 >  SRR4302537.1015119/1‑94 (MQ=60)
                                                                            CGGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTT                 <  SRR4302537.4683881/94‑1 (MQ=60)
                                                                             GGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTT                <  SRR4302537.1379387/94‑1 (MQ=60)
                                                                             GGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTT                >  SRR4302537.1475246/1‑94 (MQ=60)
                                                                             GGCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGACAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTT                >  SRR4302537.3491215/1‑94 (MQ=60)
                                                                              GCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGTCCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTT               <  SRR4302537.3165754/94‑1 (MQ=60)
                                                                              GCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTT               <  SRR4302537.992645/94‑1 (MQ=60)
                                                                              GCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTT               >  SRR4302537.5454616/1‑94 (MQ=60)
                                                                              GCGAGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTT               <  SRR4302537.2959282/94‑1 (MQ=60)
                                                                                 AGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCA            >  SRR4302537.2692629/1‑94 (MQ=60)
                                                                                 AGTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCA            <  SRR4302537.4792422/94‑1 (MQ=60)
                                                                                  GTGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCAT           <  SRR4302537.4140417/94‑1 (MQ=60)
                                                                                   TGCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATT          >  SRR4302537.5959600/1‑94 (MQ=60)
                                                                                    GCCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTT         <  SRR4302537.4180147/94‑1 (MQ=60)
                                                                                     CCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTT        <  SRR4302537.5558295/94‑1 (MQ=60)
                                                                                     CCTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTT        <  SRR4302537.2253972/94‑1 (MQ=60)
                                                                                     CCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTT        >  SRR4302537.4535224/1‑94 (MQ=60)
                                                                                      CTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTG       <  SRR4302537.275639/94‑1 (MQ=60)
                                                                                      CTGAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTG       <  SRR4302537.4234905/94‑1 (MQ=60)
                                                                                        GAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGTAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCA     <  SRR4302537.4115705/94‑1 (MQ=60)
                                                                                        GAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCA     >  SRR4302537.2672940/1‑94 (MQ=60)
                                                                                        GAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCA     <  SRR4302537.1413192/94‑1 (MQ=60)
                                                                                        GAGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCA     <  SRR4302537.3846026/94‑1 (MQ=60)
                                                                                         AGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAG    >  SRR4302537.5315937/1‑94 (MQ=60)
                                                                                         AGCCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAG    >  SRR4302537.4880771/1‑94 (MQ=60)
                                                                                           CCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGT  <  SRR4302537.3104544/94‑1 (MQ=60)
                                                                                           CCGCAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGT  <  SRR4302537.6073294/94‑1 (MQ=60)
                                                                                           CCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGT  >  SRR4302537.2751408/1‑94 (MQ=60)
                                                                                             |                                                                                           
GATTGTTGTGGCGCATAAAGCGAAGGCGTTGATGCTTCGACAACCAGCGTGTCATCCGCGTTAGTTTGCCCTTCGGCGGCGAGTGCCTGAGCCACAGGCGTGATGCCAATACAAAGACCGGCAAAGAACGTGAGCGAATGAAATTGTCTGGCAGGGAAATTGCGATTGTTTTCCATTTTGCAGGT  >  NC_000913/840579‑840763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑