Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	851,438	C→T	R128K (AGG→AAG)	ybiP ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	851,438	0	C	T	97.9%	690.3 / 0.4	195	R128K (AGG→AAG)	ybiP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (2/2); new base T (100/90); total (102/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 197 total aligned reads displayed.

ACGGGAGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCC  >  NC_000913/851348‑851527

 cGGGAGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGa                                                                                        >  2:5541596/1‑93 (MQ=255)

  gggAGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTTTCTTTTTTTCG‑CTTGAt                                                                                       <  2:2957656/93‑1 (MQ=255)

  gggAGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGAt                                                                                       >  1:5186400/1‑93 (MQ=255)

   ggAGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCTAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGAtt                                                                                      <  1:4525358/93‑1 (MQ=255)

     aGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGc                                                                                    <  1:1665602/93‑1 (MQ=255)

       caacaaAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTa                                                                                  >  2:2269610/1‑93 (MQ=255)

       caacaaAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTa                                                                                  <  2:5914168/93‑1 (MQ=255)

        aacaaAAAATGTCGCCACACTGAATACCGGACACAGGCTGTCGAAGGGATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTAt                                                                                 >  2:6444190/1‑93 (MQ=255)

         acaaAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTAtt                                                                                >  2:2818665/1‑93 (MQ=255)

          caaAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTa                                                                               <  2:2054307/93‑1 (MQ=255)

          caaAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTa                                                                               <  2:2898109/93‑1 (MQ=255)

          caaAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTa                                                                               <  2:298341/93‑1 (MQ=255)

           aaaaaaTGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTAc                                                                              <  1:998318/93‑1 (MQ=255)

            aaaaaTGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CCTGATTGCTATTACg                                                                             >  2:1892211/1‑93 (MQ=255)

              aaaTGTCGCCACACTGAATCCCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGcc                                                                           <  2:4239829/93‑1 (MQ=255)

               aaTGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCg                                                                          <  1:4075324/93‑1 (MQ=255)

               aaTGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCg                                                                          >  2:4931298/1‑93 (MQ=255)

                aTGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGa                                                                         <  2:3704785/93‑1 (MQ=255)

                     gCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCa                                                                    >  1:2982813/1‑93 (MQ=255)

                      ccACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCaa                                                                   >  1:1450434/1‑93 (MQ=255)

                      ccACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCaa                                                                   <  1:4920558/93‑1 (MQ=255)

                        acacTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCaaaa                                                                 >  1:1430626/1‑93 (MQ=255)

                          acTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAaaat                                                               <  1:2268445/93‑1 (MQ=255)

                           cTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAaaata                                                              >  2:5937025/1‑93 (MQ=255)

                           cTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAaaata                                                              >  2:5065996/1‑93 (MQ=255)

                            tGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CATGATTGCTATTACGCCGAGCGCAAaaataa                                                             <  1:2538221/93‑1 (MQ=255)

                             gAATACCAGACACAGGCTGTCGAAGGGATTCCAGCGCG‑CCTTTTCATTCTCTTTTTTTCG‑CTTGATTGATATTACGCCGAGCGCAAaaataaa                                                            >  2:5507557/1‑93 (MQ=255)

                              aaTCCCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAaaataaat                                                           <  2:90063/93‑1 (MQ=255)

                              aaTACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAaaataaat                                                           >  1:4403104/1‑93 (MQ=255)

                              aaTACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CCTGATTGCTATTACGCCGAGCGCAAaaataaat                                                           >  1:6429086/1‑93 (MQ=255)

                               aTACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAaataaata                                                          <  2:3207606/93‑1 (MQ=255)

                               aTACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAaataaata                                                          <  2:3483454/93‑1 (MQ=255)

                                tACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAataaataa                                                         <  1:35999/93‑1 (MQ=255)

                                 aCCAGACACAGGCTGTCGAAGGGATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAg                                                        >  2:4354369/1‑93 (MQ=255)

                                  ccAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGc                                                       <  2:1521079/93‑1 (MQ=255)

                                  ccAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGc                                                       <  1:6207806/93‑1 (MQ=255)

                                  ccAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGc                                                       <  2:6267698/93‑1 (MQ=255)

                                   cAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGcc                                                      >  1:1928358/1‑93 (MQ=255)

                                    aGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCg                                                     <  2:3586430/93‑1 (MQ=255)

                                      acacAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGAc                                                   <  2:93899/93‑1 (MQ=255)

                                         caGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAg                                                <  2:5750550/93‑1 (MQ=255)

                                           ggCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGt                                              >  1:1127844/1‑93 (MQ=255)

                                            gCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTa                                             <  1:3759973/93‑1 (MQ=255)

                                            gCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTa                                             <  1:1214413/93‑1 (MQ=255)

                                              tGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGc                                           <  1:727342/93‑1 (MQ=255)

                                              tGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGc                                           <  1:4707405/93‑1 (MQ=255)

                                               gTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCt                                          >  1:2697857/1‑93 (MQ=255)

                                               gTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCt                                          >  2:6478681/1‑93 (MQ=255)

                                                tCGAAGGTATTCCAGCGCGCCTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCt                                          >  1:6092022/1‑93 (MQ=255)

                                                  gAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTa                                       >  2:5183994/1‑93 (MQ=255)

                                                  gAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTa                                       <  1:876425/93‑1 (MQ=255)

                                                      gTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCAc                                   <  1:3552644/93‑1 (MQ=255)

                                                      gTAGTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCAc                                   <  1:4981335/93‑1 (MQ=255)

                                                        aTTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACgg                                 >  1:5428486/1‑93 (MQ=255)

                                                         ttCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGa                                >  2:760837/1‑93 (MQ=255)

                                                           ccAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGaaa                              >  2:4051580/1‑93 (MQ=255)

                                                             aGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGaaaaa                            >  1:5393494/1‑93 (MQ=255)

                                                             aGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGaaaaa                            >  1:2147712/1‑93 (MQ=255)

                                                             aGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGaaaaa                            >  2:2542086/1‑93 (MQ=255)

                                                              gcgcg‑cTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAAt                           >  2:3495935/1‑93 (MQ=255)

                                                              gcgcg‑cTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAAt                           >  2:3782456/1‑93 (MQ=255)

                                                              gcgcg‑cTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAAt                           >  2:1021164/1‑93 (MQ=255)

                                                               cgcg‑cTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATc                          <  2:2088592/93‑1 (MQ=255)

                                                                  g‑cTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTc                       >  2:611822/1‑93 (MQ=255)

                                                                  g‑cTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTc                       <  1:2061877/93‑1 (MQ=255)

                                                                    cTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCa                      <  2:6430851/93‑1 (MQ=255)

                                                                    cTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCa                      <  1:4357489/93‑1 (MQ=255)

                                                                    cTTTTTCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCa                      >  2:2183977/1‑93 (MQ=255)

                                                                     tttttCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAg                     >  1:2247739/1‑93 (MQ=255)

                                                                     tttttCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAg                     >  1:3361316/1‑93 (MQ=255)

                                                                     tttttCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAg                     >  2:4485977/1‑93 (MQ=255)

                                                                     tttttCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAg                     <  2:5519791/93‑1 (MQ=255)

                                                                     tttttCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAg                     <  1:5572972/93‑1 (MQ=255)

                                                                     tttttCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAg                     >  2:1884739/1‑93 (MQ=255)

                                                                      ttttCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAgg                    >  2:1110253/1‑93 (MQ=255)

                                                                      ttttCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTAAgg                    >  2:4350959/1‑93 (MQ=255)

                                                                       tttCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGa                   <  2:489498/93‑1 (MQ=255)

                                                                       tttCATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGa                   >  1:5464008/1‑93 (MQ=255)

                                                                        ttCATTCTCTTTTTTTCG‑CCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGAt                  <  1:1863201/93‑1 (MQ=255)

                                                                          cATTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGAttt                >  1:2799670/1‑93 (MQ=255)

                                                                           aTTCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAATTAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTc               >  1:1579443/1‑93 (MQ=255)

                                                                            ttCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCg              >  1:5009249/1‑93 (MQ=255)

                                                                            ttCTCTTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAAGCGTCAGGATTTCg              <  1:5698921/93‑1 (MQ=255)

                                                                              ctctTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGt            >  2:3678842/1‑93 (MQ=255)

                                                                              ctctTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGt            <  1:3094806/93‑1 (MQ=255)

                                                                              ctctTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGt            <  1:316234/93‑1 (MQ=255)

                                                                              ctctTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGt            <  2:177405/93‑1 (MQ=255)

                                                                               tctTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTc           >  1:6229967/1‑93 (MQ=255)

                                                                               tctTTTTTTCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTc           >  2:2409658/1‑93 (MQ=255)

                                                                                 tttttttCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa         >  2:1082322/1‑93 (MQ=255)

                                                                                 tttttttCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa         >  2:4689022/1‑93 (MQ=255)

                                                                                 tttttttCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa         >  2:3816222/1‑93 (MQ=255)

                                                                                 tttttttCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa         >  1:2809187/1‑93 (MQ=255)

                                                                                  ttttttCGCCCGGTTTGTTTTTACGCCAAGCCCAAAAATAATAAAGCCACCCAGGAACTTGTACCACGGAAAAATCGCAAGGATTTCGGTCgc         >  2:2570781/1‑92 (MQ=255)

                                                                                   tttttCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGAct       <  2:2334165/93‑1 (MQ=255)

                                                                                    ttttCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGActc      >  1:6385161/1‑93 (MQ=255)

                                                                                    ttttCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGAatc      >  1:1161523/1‑93 (MQ=255)

                                                                                       tCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc   <  1:961982/93‑1 (MQ=255)

                                                                                       tCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc   >  1:4639876/1‑93 (MQ=255)

                                                                                       tCG‑CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc   >  1:1485860/1‑93 (MQ=255)

ACGGGAGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCG‑CCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCC  >  NC_000913/851348‑851527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGGAGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATAT  >  NC_000913/851349‑851531

CGGGAGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGAT                                                                                           <  SRR4302537.3136233/94‑1 (MQ=60)

CGGGAGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGAT                                                                                           >  SRR4302537.5222070/1‑94 (MQ=60)

  GGAGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCTAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTT                                                                                         <  SRR4302537.4556670/94‑1 (MQ=60)

   GAGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGC                                                                                        <  SRR4302537.2806373/94‑1 (MQ=60)

    AGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCT                                                                                       <  SRR4302537.1677271/94‑1 (MQ=60)

         CAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTAC                                                                                  >  SRR4302537.4971468/1‑94 (MQ=60)

          AAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACG                                                                                 <  SRR4302537.1005208/94‑1 (MQ=60)

            AAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTTCTATTACGCC                                                                               >  SRR4302537.2822960/1‑94 (MQ=60)

              AATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGA                                                                             <  SRR4302537.4103779/94‑1 (MQ=60)

                TGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGC                                                                           <  SRR4302537.647071/94‑1 (MQ=60)

                   CGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCA                                                                        >  SRR4302537.3969760/1‑94 (MQ=60)

                   CGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCA                                                                        >  SRR4302537.3003665/1‑94 (MQ=60)

                    GCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAA                                                                       >  SRR4302537.1460540/1‑94 (MQ=60)

                     CCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAA                                                                      <  SRR4302537.4954501/94‑1 (MQ=60)

                      CACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAA                                                                     >  SRR4302537.1440586/1‑94 (MQ=60)

                       ACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAA                                                                    <  SRR4302537.5450161/94‑1 (MQ=60)

                       ACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAA                                                                    >  SRR4302537.3717639/1‑94 (MQ=60)

                       ACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAA                                                                    <  SRR4302537.1367520/94‑1 (MQ=60)

                        CACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAAT                                                                   <  SRR4302537.1194276/94‑1 (MQ=60)

                         ACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATA                                                                  >  SRR4302537.4846830/1‑94 (MQ=60)

                         ACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATA                                                                  <  SRR4302537.2284465/94‑1 (MQ=60)

                         ACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTATTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATA                                                                  >  SRR4302537.4580308/1‑94 (MQ=60)

                           TGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCGTTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAA                                                                >  SRR4302537.4499344/1‑94 (MQ=60)

                           TGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCATGATTGCTATTACGCCGAGCGCAAAAATAAA                                                                <  SRR4302537.2556028/94‑1 (MQ=60)

                            GAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAAT                                                               >  SRR4302537.4433488/1‑94 (MQ=60)

                            GAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAAT                                                               >  SRR4302537.6472767/1‑94 (MQ=60)

                              ATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAA                                                             <  SRR4302537.1457861/94‑1 (MQ=60)

                               TACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAGAAATAAATAAG                                                            <  SRR4302537.1640079/94‑1 (MQ=60)

                               TACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAG                                                            <  SRR4302537.36275/94‑1 (MQ=60)

                                ACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGC                                                           <  SRR4302537.1971515/94‑1 (MQ=60)

                                ACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGC                                                           >  SRR4302537.3579468/1‑94 (MQ=60)

                                 CCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCC                                                          >  SRR4302537.1941738/1‑94 (MQ=60)

                                 CCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCC                                                          >  SRR4302537.2639984/1‑94 (MQ=60)

                                 CCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCC                                                          <  SRR4302537.6250578/94‑1 (MQ=60)

                                  CAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCG                                                         >  SRR4302537.5551432/1‑94 (MQ=60)

                                     ACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACC                                                      <  SRR4302537.4685214/94‑1 (MQ=60)

                                      CGCAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCA                                                     <  SRR4302537.6261037/94‑1 (MQ=60)

                                      CACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTATGCCGAGCGCCAAAATAAATAAGCCGACCA                                                     >  SRR4302537.1461620/1‑94 (MQ=60)

                                         AGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGT                                                  >  SRR4302537.1135668/1‑94 (MQ=60)

                                           GCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAG                                                >  SRR4302537.1559272/1‑94 (MQ=60)

                                           GCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAG                                                <  SRR4302537.3786370/94‑1 (MQ=60)

                                           GCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAG                                                <  SRR4302537.1936845/94‑1 (MQ=60)

                                           GCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAG                                                <  SRR4302537.1222862/94‑1 (MQ=60)

                                             TGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCT                                              >  SRR4302537.2716703/1‑94 (MQ=60)

                                             TGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCT                                              <  SRR4302537.732309/94‑1 (MQ=60)

                                             TGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCT                                              <  SRR4302537.4739973/94‑1 (MQ=60)

                                             TGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCT                                              <  SRR4302537.210979/94‑1 (MQ=60)

                                             TGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCT                                              >  SRR4302537.884083/1‑94 (MQ=60)

                                             TGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCT                                              <  SRR4302537.5706744/94‑1 (MQ=60)

                                             TGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCT                                              <  SRR4302537.6214665/94‑1 (MQ=60)

                                              GTCGAAGGTATTCCAGCGCGCCTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCT                                              >  SRR4302537.6133879/1‑94 (MQ=60)

                                                 GAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTAC                                          <  SRR4302537.882454/94‑1 (MQ=60)

                                                     GTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACG                                      <  SRR4302537.3577728/94‑1 (MQ=60)

                                                     GTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACG                                      >  SRR4302537.1834093/1‑94 (MQ=60)

                                                     GTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACG                                      >  SRR4302537.732953/1‑94 (MQ=60)

                                                     GTAGTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACG                                      <  SRR4302537.5015670/94‑1 (MQ=60)

                                                      TATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGG                                     >  SRR4302537.5465500/1‑94 (MQ=60)

                                                       ATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGA                                    <  SRR4302537.3050264/94‑1 (MQ=60)

                                                          CCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAA                                 >  SRR4302537.6115168/1‑94 (MQ=60)

                                                           CAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAA                                >  SRR4302537.2162819/1‑94 (MQ=60)

                                                           CAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAA                                >  SRR4302537.5430330/1‑94 (MQ=60)

                                                             GCGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATC                              <  SRR4302537.2340702/94‑1 (MQ=60)

                                                              CGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCG                             <  SRR4302537.3413035/94‑1 (MQ=60)

                                                              CGCG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCG                             >  SRR4302537.4347121/1‑94 (MQ=60)

                                                                CG‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTC                           >  SRR4302537.1005027/1‑94 (MQ=60)

                                                                 G‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCA                          >  SRR4302537.5472149/1‑94 (MQ=60)

                                                                 G‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCA                          <  SRR4302537.2076358/94‑1 (MQ=60)

                                                                 G‑CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCA                          >  SRR4302537.5352028/1‑94 (MQ=60)

                                                                   CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAG                         >  SRR4302537.3385125/1‑94 (MQ=60)

                                                                   CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAG                         >  SRR4302537.2263600/1‑94 (MQ=60)

                                                                   CTTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAG                         <  SRR4302537.4387651/94‑1 (MQ=60)

                                                                    TTTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGG                        <  SRR4302537.5611063/94‑1 (MQ=60)

                                                                     TTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGA                       >  SRR4302537.5501277/1‑94 (MQ=60)

                                                                     TTTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGA                       >  SRR4302537.3187583/1‑94 (MQ=60)

                                                                      TTTCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGAT                      >  SRR4302537.5038733/1‑94 (MQ=60)

                                                                      TTTCATTCTCTTTCTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGAT                      <  SRR4302537.6289840/94‑1 (MQ=60)

                                                                       TTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATT                     <  SRR4302537.1876177/94‑1 (MQ=60)

                                                                        TCATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTT                    >  SRR4302537.2819244/1‑94 (MQ=60)

                                                                         CATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAATTAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTC                   >  SRR4302537.1590480/1‑94 (MQ=60)

                                                                          ATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCG                  >  SRR4302537.5043757/1‑94 (MQ=60)

                                                                          ATTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCG                  <  SRR4302537.2283817/94‑1 (MQ=60)

                                                                           TTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCCGGATTTCGG                 >  SRR4302537.4094521/1‑94 (MQ=60)

                                                                           TTCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAAGCGTCAGGATTTCGG                 <  SRR4302537.5737919/94‑1 (MQ=60)

                                                                            TCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGT                >  SRR4302537.2214990/1‑94 (MQ=60)

                                                                            TCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGT                <  SRR4302537.6520122/94‑1 (MQ=60)

                                                                            TCTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGT                >  SRR4302537.28327/1‑94 (MQ=60)

                                                                             CTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTC               <  SRR4302537.318429/94‑1 (MQ=60)

                                                                             CTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTC               <  SRR4302537.3116395/94‑1 (MQ=60)

                                                                             CTCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTC               >  SRR4302537.6272825/1‑94 (MQ=60)

                                                                              TCTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCG              >  SRR4302537.4405405/1‑94 (MQ=60)

                                                                               CTTTTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGA             >  SRR4302537.2828825/1‑94 (MQ=60)

                                                                                  TTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTC          >  SRR4302537.6428678/1‑94 (MQ=60)

                                                                                  TTTTTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGAATC          >  SRR4302537.1169600/1‑94 (MQ=60)

                                                                                     TTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTC       >  SRR4302537.4671960/1‑94 (MQ=60)

                                                                                     TTCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTC       >  SRR4302537.1496197/1‑94 (MQ=60)

                                                                                      TCGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCC      <  SRR4302537.968622/94‑1 (MQ=60)

                                                                                       CGCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAGAATCGTCAGGATTTCGGTCGACTCTTCCA     >  SRR4302537.1399776/1‑94 (MQ=60)

                                                                                        GCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCAT    <  SRR4302537.5040838/94‑1 (MQ=60)

                                                                                        GCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCAT    >  SRR4302537.4917303/1‑94 (MQ=60)

                                                                                        GCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCAT    >  SRR4302537.4906000/1‑94 (MQ=60)

                                                                                        GCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCAT    >  SRR4302537.2717218/1‑94 (MQ=60)

                                                                                        GCTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCAT    <  SRR4302537.2250185/94‑1 (MQ=60)

                                                                                         CTTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATA   >  SRR4302537.4358841/1‑94 (MQ=60)

                                                                                          TTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATAT  <  SRR4302537.209545/94‑1 (MQ=60)

CGGGAGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCG‑CTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATAT  >  NC_000913/851349‑851531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`