Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 858,619 T→C V211V (GTA→GTG)  b0822 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913858,6190TC97.0% 880.7 / 10.7 271V211V (GTA→GTG) b0822hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (5/3);  new base C (114/149);  total (119/152)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.89e-01

Only 100 of 280 total aligned reads displayed.

TTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCTACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAA  >  NC_000913/858529‑858708
                                                                                          |                                                                                         
 tGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCAcc                                                                                        >  2:2800034/1‑93 (MQ=255)
  gTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGccacca                                                                                       <  2:2312418/93‑1 (MQ=255)
   tttAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCcaccac                                                                                      >  1:5053904/1‑93 (MQ=255)
    ttAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACcaca                                                                                     >  2:3253387/1‑93 (MQ=255)
      aaTGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACAtt                                                                                   >  2:6412102/1‑93 (MQ=255)
        tGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCTCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACAtttt                                                                                 <  1:2479753/93‑1 (MQ=255)
        tGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACAtttt                                                                                 <  1:4209770/93‑1 (MQ=255)
        tGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACAtttt                                                                                 <  2:802401/93‑1 (MQ=255)
         gTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTg                                                                                >  1:4261725/1‑93 (MQ=255)
           tttCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCg                                                                              >  1:3167226/1‑93 (MQ=255)
                 cagcaTTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACa                                                                        <  2:5867056/93‑1 (MQ=255)
                 cagcaTTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACa                                                                        <  1:4622313/93‑1 (MQ=255)
                  agcaTTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAg                                                                       <  1:2220480/93‑1 (MQ=255)
                  agcaTTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAg                                                                       <  1:275171/93‑1 (MQ=255)
                  agcaTTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAg                                                                       >  2:2293944/1‑93 (MQ=255)
                  agcaTTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAg                                                                       <  2:5928091/93‑1 (MQ=255)
                   gcaTTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGa                                                                      >  1:5458173/1‑93 (MQ=255)
                   gcaTTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGa                                                                      <  1:2981572/93‑1 (MQ=255)
                   gcaTTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGa                                                                      <  2:4435066/93‑1 (MQ=255)
                   gcaTTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGa                                                                      >  2:919217/1‑93 (MQ=255)
                     aTTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATc                                                                    >  1:1112844/1‑93 (MQ=255)
                        gCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCa                                                                 <  2:3594541/93‑1 (MQ=255)
                          ccATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGc                                                               <  2:5389246/93‑1 (MQ=255)
                           cATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCg                                                              >  1:3932162/1‑93 (MQ=255)
                            aTCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGt                                                             <  2:5790991/93‑1 (MQ=255)
                            aTCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGt                                                             >  1:649604/1‑93 (MQ=255)
                             tCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGtt                                                            >  1:3847353/1‑93 (MQ=255)
                             tCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGtt                                                            >  1:1009657/1‑93 (MQ=255)
                             tCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGtt                                                            <  2:6332425/93‑1 (MQ=255)
                             tCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGtt                                                            >  2:3383080/1‑93 (MQ=255)
                               gCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTc                                                          <  2:1154553/93‑1 (MQ=255)
                               gCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTc                                                          <  1:5876549/93‑1 (MQ=255)
                                cAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCGAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCa                                                         <  2:1706632/93‑1 (MQ=255)
                                cAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCa                                                         <  2:387219/93‑1 (MQ=255)
                                  aaGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGt                                                       <  2:2743915/93‑1 (MQ=255)
                                  aaGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGt                                                       >  2:2234180/1‑93 (MQ=255)
                                   aGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCTACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTa                                                      <  1:43509/93‑1 (MQ=255)
                                     gAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAAc                                                    <  1:1611179/93‑1 (MQ=255)
                                      aaTAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAAcc                                                   >  1:89999/1‑93 (MQ=255)
                                      aaTAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAAcc                                                   <  1:3982803/93‑1 (MQ=255)
                                       aTAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACcg                                                  <  2:5653287/93‑1 (MQ=255)
                                       aTAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACcg                                                  <  2:5967509/93‑1 (MQ=255)
                                        tAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACcgc                                                 <  2:1208942/93‑1 (MQ=255)
                                         aaCGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACcgcg                                                <  1:3597645/93‑1 (MQ=255)
                                            gcgcCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGaaa                                             >  2:5534688/1‑93 (MQ=255)
                                             cgcCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAAt                                            <  1:2012975/93‑1 (MQ=255)
                                              gcCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATa                                           <  1:5816876/93‑1 (MQ=255)
                                              gcCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATa                                           <  1:2086170/93‑1 (MQ=255)
                                              gcCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATa                                           <  2:6456890/93‑1 (MQ=255)
                                               ccATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATAc                                          <  2:3135152/93‑1 (MQ=255)
                                               ccATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATAc                                          <  1:1657576/93‑1 (MQ=255)
                                                cATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATAcc                                         <  2:2506594/93‑1 (MQ=255)
                                                 aTTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCg                                        >  1:130113/1‑93 (MQ=255)
                                                  ttttCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCTACCACATTTTGCGGTTACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGt                                       >  1:4402436/1‑93 (MQ=255)
                                                    ttCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGttt                                     <  2:3058905/93‑1 (MQ=255)
                                                    ttCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGttt                                     <  1:4146813/93‑1 (MQ=255)
                                                    ttCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGttt                                     <  1:6286397/93‑1 (MQ=255)
                                                     tCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTg                                    <  1:636215/93‑1 (MQ=255)
                                                     tCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTg                                    <  1:3053921/93‑1 (MQ=255)
                                                      cAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGc                                   <  2:4373225/93‑1 (MQ=255)
                                                      cAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGc                                   <  1:5619176/93‑1 (MQ=255)
                                                       aGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCt                                  >  2:4963718/1‑93 (MQ=255)
                                                          aTCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTa                               >  2:1845017/1‑93 (MQ=255)
                                                          aTCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTa                               <  1:5173467/93‑1 (MQ=255)
                                                           tctcCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTAt                              >  2:1069564/1‑93 (MQ=255)
                                                              ccGCATCGTTACCGCTGTCGCCAATCTCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTa                           <  1:236992/93‑1 (MQ=255)
                                                              ccGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTa                           <  1:3727517/93‑1 (MQ=255)
                                                              ccGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTa                           <  1:5100271/93‑1 (MQ=255)
                                                               cGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAg                          <  2:2543905/93‑1 (MQ=255)
                                                                gCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGa                         >  2:3688651/1‑93 (MQ=255)
                                                                gCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGa                         <  2:1436982/93‑1 (MQ=255)
                                                                 cATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGAc                        >  2:3739700/1‑93 (MQ=255)
                                                                 cATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGAc                        <  2:5312848/93‑1 (MQ=255)
                                                                  aTCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGAcc                       >  1:4834714/1‑93 (MQ=255)
                                                                   tCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCg                      >  1:5962144/1‑93 (MQ=255)
                                                                   tCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCg                      >  1:2781514/1‑93 (MQ=255)
                                                                   tCGTTACCGCTGTCGCCAATCGCCACCAAATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGTCCg                      >  1:6370861/1‑93 (MQ=255)
                                                                    cGTAACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCgg                     <  2:6470220/93‑1 (MQ=255)
                                                                     gTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCggg                    >  2:100121/1‑93 (MQ=255)
                                                                     gTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCggg                    >  2:2958322/1‑93 (MQ=255)
                                                                       tACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGaa                  <  2:6125148/93‑1 (MQ=255)
                                                                        aCCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGaat                 <  2:5130108/93‑1 (MQ=255)
                                                                         ccGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGaata                >  1:4465168/1‑93 (MQ=255)
                                                                         ccGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGaata                >  2:1303812/1‑93 (MQ=255)
                                                                         ccGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGaata                >  2:3985769/1‑93 (MQ=255)
                                                                            cTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGCCCGGGAATAATc             <  2:3910486/93‑1 (MQ=255)
                                                                            cTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGTAATAATc             >  1:570630/1‑93 (MQ=255)
                                                                             tGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCa            <  2:4601823/93‑1 (MQ=255)
                                                                              gTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAg           <  2:4784955/93‑1 (MQ=255)
                                                                                cGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGt         <  1:1908712/93‑1 (MQ=255)
                                                                                 gCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTc        >  1:4043106/1‑93 (MQ=255)
                                                                                 gCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTc        <  1:2619076/93‑1 (MQ=255)
                                                                                   cAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGa      >  1:6096660/1‑93 (MQ=255)
                                                                                   cAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGa      >  1:1453231/1‑93 (MQ=255)
                                                                                    aaTCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGAt     >  1:778745/1‑93 (MQ=255)
                                                                                    aaTCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGAt     >  1:1549586/1‑93 (MQ=255)
                                                                                    aaTCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGAt     >  2:154190/1‑93 (MQ=255)
                                                                                     aTCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATa    >  2:4212865/1‑93 (MQ=255)
                                                                                      tCGCTACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATaa   >  1:3418329/1‑93 (MQ=255)
                                                                                      tCGCTACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATaa   >  2:285013/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCTACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAA  >  NC_000913/858529‑858708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCTACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAAGCCA  >  NC_000913/858526‑858712
                                                                                             |                                                                                             
GATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCC                                                                                               >  SRR4302537.2034561/1‑94 (MQ=60)
GATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCC                                                                                               >  SRR4302537.3988649/1‑94 (MQ=60)
 ATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCA                                                                                              >  SRR4302537.683442/1‑94 (MQ=60)
 ATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCA                                                                                              >  SRR4302537.4193860/1‑94 (MQ=60)
 ATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCA                                                                                              >  SRR4302537.250561/1‑94 (MQ=60)
  TTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCTAC                                                                                             >  SRR4302537.849782/1‑94 (MQ=60)
  TTTGTTTAATGTTTTCAGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCAC                                                                                             >  SRR4302537.431157/1‑94 (MQ=60)
    TGTTTAATGTTTTCCGCAGCATTGCCCATCGCAACGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCA                                                                                           >  SRR4302537.6410104/1‑94 (MQ=60)
     GTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCAC                                                                                          >  SRR4302537.5088698/1‑94 (MQ=60)
      TTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACA                                                                                         >  SRR4302537.258165/1‑94 (MQ=60)
       TTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACAT                                                                                        <  SRR4302537.2090673/94‑1 (MQ=60)
        TAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATT                                                                                       <  SRR4302537.6370354/94‑1 (MQ=60)
        TAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATT                                                                                       <  SRR4302537.3232956/94‑1 (MQ=60)
        TAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATT                                                                                       >  SRR4302537.4251501/1‑94 (MQ=60)
           TGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCTCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTG                                                                                    <  SRR4302537.2497185/94‑1 (MQ=60)
           TGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTG                                                                                    <  SRR4302537.1710886/94‑1 (MQ=60)
           TGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTG                                                                                    <  SRR4302537.4239137/94‑1 (MQ=60)
           TGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTG                                                                                    >  SRR4302537.4291339/1‑94 (MQ=60)
             TTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCG                                                                                  >  SRR4302537.3189473/1‑94 (MQ=60)
                 CCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGA                                                                              >  SRR4302537.5322581/1‑94 (MQ=60)
                  CGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGAC                                                                             >  SRR4302537.6053017/1‑94 (MQ=60)
                    CAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAG                                                                           <  SRR4302537.4654311/94‑1 (MQ=60)
                    CAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAG                                                                           <  SRR4302537.6228568/94‑1 (MQ=60)
                     AGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGA                                                                          <  SRR4302537.2236127/94‑1 (MQ=60)
                     AGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGA                                                                          <  SRR4302537.2673819/94‑1 (MQ=60)
                     AGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGA                                                                          <  SRR4302537.277100/94‑1 (MQ=60)
                     AGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGA                                                                          >  SRR4302537.3213646/1‑94 (MQ=60)
                     AGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGA                                                                          >  SRR4302537.5495402/1‑94 (MQ=60)
                     AGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGA                                                                          >  SRR4302537.746804/1‑94 (MQ=60)
                     AGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGA                                                                          >  SRR4302537.3998906/1‑94 (MQ=60)
                      GCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGAT                                                                         <  SRR4302537.3002423/94‑1 (MQ=60)
                      GCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGAT                                                                         >  SRR4302537.5119749/1‑94 (MQ=60)
                       CATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATC                                                                        >  SRR4302537.4237907/1‑94 (MQ=60)
                       CATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATC                                                                        >  SRR4302537.1120527/1‑94 (MQ=60)
                        ATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCC                                                                       <  SRR4302537.307281/94‑1 (MQ=60)
                        ATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGAATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCC                                                                       >  SRR4302537.2270929/1‑94 (MQ=60)
                          TGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATACCA                                                                     >  SRR4302537.6196522/1‑94 (MQ=60)
                           GCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCTACCACATTTTGCGGTGACAGATCCCAG                                                                    <  SRR4302537.2785646/94‑1 (MQ=60)
                            CCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGC                                                                   <  SRR4302537.1142999/94‑1 (MQ=60)
                            CCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGC                                                                   >  SRR4302537.3876740/1‑94 (MQ=60)
                             CCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCG                                                                  >  SRR4302537.154507/1‑94 (MQ=60)
                             CCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCG                                                                  >  SRR4302537.3959637/1‑94 (MQ=60)
                              CATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGT                                                                 >  SRR4302537.654031/1‑94 (MQ=60)
                               ATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTT                                                                >  SRR4302537.1016620/1‑94 (MQ=60)
                               ATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTT                                                                >  SRR4302537.3874287/1‑94 (MQ=60)
                               ATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTT                                                                <  SRR4302537.6382174/94‑1 (MQ=60)
                                TCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTT                                                               >  SRR4302537.2895647/1‑94 (MQ=60)
                                 CGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTC                                                              >  SRR4302537.349591/1‑94 (MQ=60)
                                 CGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTC                                                              >  SRR4302537.3992887/1‑94 (MQ=60)
                                  GCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCA                                                             <  SRR4302537.5916868/94‑1 (MQ=60)
                                  GCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCA                                                             >  SRR4302537.5912113/1‑94 (MQ=60)
                                  GCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCA                                                             <  SRR4302537.4642143/94‑1 (MQ=60)
                                   CAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAG                                                            <  SRR4302537.1053826/94‑1 (MQ=60)
                                    AAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGT                                                           >  SRR4302537.2796269/1‑94 (MQ=60)
                                     AAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTA                                                          >  SRR4302537.5143560/1‑94 (MQ=60)
                                     AAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTA                                                          >  SRR4302537.2398344/1‑94 (MQ=60)
                                      AGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCTACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAA                                                         <  SRR4302537.43833/94‑1 (MQ=60)
                                      AGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAA                                                         <  SRR4302537.5707938/94‑1 (MQ=60)
                                      AGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAA                                                         <  SRR4302537.4280924/94‑1 (MQ=60)
                                      AGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAA                                                         <  SRR4302537.1968021/94‑1 (MQ=60)
                                        GAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACC                                                       >  SRR4302537.90658/1‑94 (MQ=60)
                                        GAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACC                                                       >  SRR4302537.4389397/1‑94 (MQ=60)
                                        GAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACC                                                       <  SRR4302537.1622439/94‑1 (MQ=60)
                                         AATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCG                                                      <  SRR4302537.3139128/94‑1 (MQ=60)
                                         AATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCG                                                      <  SRR4302537.5236315/94‑1 (MQ=60)
                                         AATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCG                                                      <  SRR4302537.4010685/94‑1 (MQ=60)
                                          ATAGCGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGC                                                     <  SRR4302537.600500/94‑1 (MQ=60)
                                          ATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGC                                                     >  SRR4302537.462634/1‑94 (MQ=60)
                                          ATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGGCGCCAAGCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGC                                                     >  SRR4302537.5123514/1‑94 (MQ=60)
                                            AACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGA                                                   <  SRR4302537.3623002/94‑1 (MQ=60)
                                            AACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGA                                                   >  SRR4302537.6442522/1‑94 (MQ=60)
                                                tgtCATTTTCAGCATCTCCGCATCGTTCCCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATA                                               <  SRR4302537.5661390/91‑1 (MQ=60)
                                                CGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATA                                               <  SRR4302537.1548995/94‑1 (MQ=60)
                                                CGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATA                                               <  SRR4302537.2027005/94‑1 (MQ=60)
                                                CGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATA                                               <  SRR4302537.374516/94‑1 (MQ=60)
                                                 GCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATAC                                              <  SRR4302537.5605828/94‑1 (MQ=60)
                                                 GCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATAC                                              <  SRR4302537.5962337/94‑1 (MQ=60)
                                                 GCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATAC                                              <  SRR4302537.2100879/94‑1 (MQ=60)
                                                 GCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATAC                                              <  SRR4302537.275954/94‑1 (MQ=60)
                                                 GCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATAC                                              <  SRR4302537.5856746/94‑1 (MQ=60)
                                                  CCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACC                                             <  SRR4302537.1669192/94‑1 (MQ=60)
                                                  CCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACC                                             <  SRR4302537.5980043/94‑1 (MQ=60)
                                                  CCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACC                                             >  SRR4302537.403359/1‑94 (MQ=60)
                                                   CATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCG                                            >  SRR4302537.131035/1‑94 (MQ=60)
                                                    ATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCTACCACATTTTGCGGTTACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGT                                           >  SRR4302537.4432814/1‑94 (MQ=60)
                                                     TTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTT                                          <  SRR4302537.4939075/94‑1 (MQ=60)
                                                     TTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTT                                          <  SRR4302537.2194746/94‑1 (MQ=60)
                                                      TTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTT                                         <  SRR4302537.1621235/94‑1 (MQ=60)
                                                       TTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTG                                        <  SRR4302537.4175749/94‑1 (MQ=60)
                                                       TTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTG                                        <  SRR4302537.6329494/94‑1 (MQ=60)
                                                        TCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGC                                       <  SRR4302537.3075204/94‑1 (MQ=60)
                                                        TCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGC                                       <  SRR4302537.640541/94‑1 (MQ=60)
                                                         CAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCT                                      <  SRR4302537.5657600/94‑1 (MQ=60)
                                                         CAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCT                                      <  SRR4302537.4774476/94‑1 (MQ=60)
                                                         CAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCT                                      <  SRR4302537.4418347/94‑1 (MQ=60)
                                                         CAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCT                                      <  SRR4302537.3016573/94‑1 (MQ=60)
                                                          AGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTT                                     >  SRR4302537.3902613/1‑94 (MQ=60)
                                                          AGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTT                                     >  SRR4302537.1459211/1‑94 (MQ=60)
                                                             ATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTAT                                  >  SRR4302537.3563367/1‑94 (MQ=60)
                                                             ATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTAT                                  <  SRR4302537.5209079/94‑1 (MQ=60)
                                                             ATCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTAT                                  <  SRR4302537.2444614/94‑1 (MQ=60)
                                                              TCTCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATG                                 <  SRR4302537.3861016/94‑1 (MQ=60)
                                                                TCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTA                               <  SRR4302537.5583526/94‑1 (MQ=60)
                                                                TCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTA                               >  SRR4302537.584284/1‑94 (MQ=60)
                                                                TCCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTA                               <  SRR4302537.3766512/94‑1 (MQ=60)
                                                                 CCGCATCGTTACCGCTGTCGCCAATCTCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAG                              <  SRR4302537.238662/94‑1 (MQ=60)
                                                                 CCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAG                              <  SRR4302537.5135412/94‑1 (MQ=60)
                                                                 CCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAG                              <  SRR4302537.3753703/94‑1 (MQ=60)
                                                                 CCGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAG                              <  SRR4302537.4183849/94‑1 (MQ=60)
                                                                  CGCATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATAACGTTTGCTCTATGTAGA                             >  SRR4302537.1211449/1‑94 (MQ=60)
                                                                    CATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACC                           >  SRR4302537.3171487/1‑94 (MQ=60)
                                                                    CATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACC                           <  SRR4302537.4474609/94‑1 (MQ=60)
                                                                    CATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACC                           >  SRR4302537.4868098/1‑94 (MQ=60)
                                                                    CATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACC                           <  SRR4302537.2202528/94‑1 (MQ=60)
                                                                     ATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCG                          >  SRR4302537.6003066/1‑94 (MQ=60)
                                                                     ATCGTTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCG                          >  SRR4302537.2800959/1‑94 (MQ=60)
                                                                     ATCGTTACCGCTGTCGCCAATCGCCACCAAATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGTCCG                          >  SRR4302537.6414309/1‑94 (MQ=60)
                                                                         TTACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAA                      >  SRR4302537.1591962/1‑94 (MQ=60)
                                                                           ACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATA                    >  SRR4302537.644381/1‑94 (MQ=60)
                                                                           ACCGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATA                    >  SRR4302537.4496049/1‑94 (MQ=60)
                                                                             CGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAAT                  <  SRR4302537.988228/94‑1 (MQ=60)
                                                                             CGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAAT                  >  SRR4302537.4348161/1‑94 (MQ=60)
                                                                             CGCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAAT                  <  SRR4302537.4080926/94‑1 (MQ=60)
                                                                              GCTGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGTAATAATC                 >  SRR4302537.574548/1‑94 (MQ=60)
                                                                                TGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAG               >  SRR4302537.581285/1‑94 (MQ=60)
                                                                                TGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAG               <  SRR4302537.920359/94‑1 (MQ=60)
                                                                                TGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAG               >  SRR4302537.2039176/1‑94 (MQ=60)
                                                                                TGTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAG               <  SRR4302537.4968644/94‑1 (MQ=60)
                                                                                 GTCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGG              <  SRR4302537.2590431/94‑1 (MQ=60)
                                                                                  TCGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGT             <  SRR4302537.126898/94‑1 (MQ=60)
                                                                                   CGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTC            >  SRR4302537.4071379/1‑94 (MQ=60)
                                                                                   CGCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTC            <  SRR4302537.1921964/94‑1 (MQ=60)
                                                                                    GCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCG           <  SRR4302537.868523/94‑1 (MQ=60)
                                                                                    GCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCG           <  SRR4302537.2637382/94‑1 (MQ=60)
                                                                                    GCCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCG           >  SRR4302537.1439030/1‑94 (MQ=60)
                                                                                     CCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGA          >  SRR4302537.6138549/1‑94 (MQ=60)
                                                                                     CCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGA          >  SRR4302537.1463362/1‑94 (MQ=60)
                                                                                     CCAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGA          >  SRR4302537.170220/1‑94 (MQ=60)
                                                                                      CAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGAT         >  SRR4302537.784090/1‑94 (MQ=60)
                                                                                      CAATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGAT         >  SRR4302537.1560401/1‑94 (MQ=60)
                                                                                        ATCGCTACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAA       >  SRR4302537.3442526/1‑94 (MQ=60)
                                                                                        ATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAA       >  SRR4302537.4028635/1‑94 (MQ=60)
                                                                                        ATCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAA       <  SRR4302537.1953887/94‑1 (MQ=60)
                                                                                        ACCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAA       <  SRR4302537.4715158/94‑1 (MQ=60)
                                                                                         TCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAA      <  SRR4302537.1630706/94‑1 (MQ=60)
                                                                                         TCGCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAA      >  SRR4302537.782666/1‑94 (MQ=60)
                                                                                          CGCCACCACATTTTGCGGTGACAGAT‑CCAGCGTTTCAGTAACCGCGAAATACCGCTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAAGC    >  SRR4302537.3638908/1‑94 (MQ=60)
                                                                                           GCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAAGC    <  SRR4302537.1643215/94‑1 (MQ=60)
                                                                                           GCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAAGC    <  SRR4302537.2176658/94‑1 (MQ=60)
                                                                                           GCCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAAGC    <  SRR4302537.5869621/94‑1 (MQ=60)
                                                                                            CCACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAAGCC   <  SRR4302537.5093762/94‑1 (MQ=60)
                                                                                             CACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAAGCCA  >  SRR4302537.1332589/1‑94 (MQ=60)
                                                                                             CACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAAGCCA  >  SRR4302537.5198260/1‑94 (MQ=60)
                                                                                             |                                                                                             
GATTTGTTTAATGTTTTCCGCAGCATTGCCCATCGCAAAGGAATAACGCGCCATTTTCAGCATCTCCGCATCGTTACCGCTGTCGCCAATCGCTACCACATTTTGCGGTGACAGATCCCAGCGTTTCAGTAACCGCGAAATACCGTTTGCTTTATGTAGACCGGGAATAATCAGGTCGATAAAGCCA  >  NC_000913/858526‑858712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑