Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 883,572 T→C L226P (CTG→CCG)  cmr → proton motive force efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913883,5720TC98.2% 358.4 / ‑2.3 111L226P (CTG→CCG) cmrproton motive force efflux pump
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (40/69);  total (40/71)
Fisher's exact test for biased strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

Only 100 of 185 total aligned reads displayed.

CCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAA  >  NC_000913/883482‑883663
                                                                                          |                                                                                            
ccACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggcgccgg                                                                                            <  2:3979419/93‑1 (MQ=255)
ccACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGCGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggcgccgg                                                                                            >  2:2060535/1‑93 (MQ=255)
  aCGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggggccggcg                                                                                          >  1:1390454/1‑93 (MQ=255)
  aCGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggggccggcg                                                                                          >  1:4657168/1‑93 (MQ=255)
  aCGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggcgccggcg                                                                                          >  1:3732702/1‑93 (MQ=255)
   cgcgTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggcgccggcgc                                                                                         <  1:3453448/93‑1 (MQ=255)
   cgcgTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggcgccggcgc                                                                                         >  1:6199867/1‑93 (MQ=255)
    gcgTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggggccggcgcg                                                                                        >  1:5059943/1‑92 (MQ=255)
    gcgTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggcgccggcgct                                                                                        >  2:5854915/1‑93 (MQ=255)
     cgTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGgcgccggcgctg                                                                                       <  1:3029327/93‑1 (MQ=255)
      gTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGggccggcgctgg                                                                                      >  2:112104/1‑93 (MQ=255)
      gTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGcgccggcgctgg                                                                                      <  1:4102010/93‑1 (MQ=255)
         taGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGGCGGCGCTGGGAt                                                                                   >  2:3878524/1‑93 (MQ=255)
         taGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGAt                                                                                   <  1:5429270/93‑1 (MQ=255)
            gCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCg                                                                                <  1:2100844/93‑1 (MQ=255)
             cGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCCGCGCTGGGGTTCGt                                                                               >  2:189173/1‑93 (MQ=255)
              gagaAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGGCGGCGCTGGGGTTCGtt                                                                              >  1:4156832/1‑93 (MQ=255)
              gagaAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGGTTCGtt                                                                              >  1:1892368/1‑93 (MQ=255)
                 aaaCTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGGTTTGTTAGt                                                                           >  2:4475742/1‑93 (MQ=255)
                 aaaCTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGt                                                                           <  2:5408951/93‑1 (MQ=255)
                  aaCTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGATTCGTTAGtt                                                                          >  1:1816736/1‑92 (MQ=255)
                  aaCTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTc                                                                          <  2:4798333/93‑1 (MQ=255)
                  aaCTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTc                                                                          <  2:818131/93‑1 (MQ=255)
                   aCTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCt                                                                         >  2:6146023/1‑93 (MQ=255)
                    cTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGGGTTGTTAGtgtg                                                                        >  1:1912472/1‑93 (MQ=255)
                    cTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCCGGGGTTCGTTAGTCTg                                                                        >  2:4981005/1‑93 (MQ=255)
                       tCACTGAAAGAACGCGGCCGTAACTATAGGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCg                                                                     <  2:57888/93‑1 (MQ=255)
                        cACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGt                                                                    <  2:5095969/93‑1 (MQ=255)
                         aCTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCCGGGGTTCGTTAGTTTGCCGtt                                                                   >  1:3839264/1‑93 (MQ=255)
                          cTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGGGCTGGGGTTCGTTAGTCTGCCGTTg                                                                  >  1:1639923/1‑93 (MQ=255)
                          cTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTTGTTAGTTTGGCGttt                                                                  >  1:101586/1‑92 (MQ=255)
                           tGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGc                                                                 >  2:2502140/1‑93 (MQ=255)
                           tGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGc                                                                 >  1:4286122/1‑93 (MQ=255)
                           tGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGc                                                                 <  2:2858229/93‑1 (MQ=255)
                            gAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCt                                                                >  1:4563959/1‑93 (MQ=255)
                            gAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCt                                                                <  2:4558727/93‑1 (MQ=255)
                             aaaGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTg                                                               >  1:3813536/1‑93 (MQ=255)
                             aaaGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTg                                                               <  2:385307/93‑1 (MQ=255)
                               aGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGc                                                             >  2:4218179/1‑93 (MQ=255)
                               aGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGGCCGCGCTGTGATTCGTTTGTCTGCCGCTGCTGGc                                                             >  1:6072261/1‑93 (MQ=255)
                               aGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGc                                                             <  1:4808698/93‑1 (MQ=255)
                               aGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGc                                                             <  1:4591304/93‑1 (MQ=255)
                                gAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGGTGCTGGCg                                                            >  2:2888187/1‑93 (MQ=255)
                                gAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCg                                                            >  2:3655343/1‑93 (MQ=255)
                                 aaCTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCgg                                                           >  2:1663900/1‑92 (MQ=255)
                                 aaCTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGt                                                           <  1:4501284/93‑1 (MQ=255)
                                 aaCTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGt                                                           <  2:2043502/93‑1 (MQ=255)
                                   cTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTgg                                                         <  1:2768792/93‑1 (MQ=255)
                                    tCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGTggg                                                        >  2:6179717/1‑92 (MQ=255)
                                     cGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑At                                                      <  2:3264887/93‑1 (MQ=255)
                                      ggTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATc                                                     <  2:1956758/93‑1 (MQ=255)
                                       gTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGATTCGTTAGTCTGGCGGTTGTGGCGGGG‑GTCg                                                    >  2:2586831/1‑93 (MQ=255)
                                         cGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGcc                                                  >  1:5904821/1‑93 (MQ=255)
                                           tGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCa                                                <  1:102763/93‑1 (MQ=255)
                                           tGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCa                                                <  1:2431349/93‑1 (MQ=255)
                                            gACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGccccg                                               >  1:805061/1‑91 (MQ=255)
                                             aCTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGTGG‑GTCGCCCAGt                                              >  2:3131792/1‑93 (MQ=255)
                                             aCTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGGGG‑GTCCGCCAgc                                              >  1:4795936/1‑92 (MQ=255)
                                             aCTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGt                                              <  2:6474796/93‑1 (MQ=255)
                                             aCTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGt                                              <  2:561636/93‑1 (MQ=255)
                                               tataAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCg                                            >  2:6422148/1‑93 (MQ=255)
                                               tataAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGGTGCTGGCGTGG‑GTCGGCCCGTCg                                            >  2:570477/1‑93 (MQ=255)
                                                 taAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGcc                                          <  2:2148552/93‑1 (MQ=255)
                                                 taAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGcc                                          <  1:527515/93‑1 (MQ=255)
                                                      tGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑GACGCCCAGTCGCCGAttt                                     >  2:6432468/1‑92 (MQ=255)
                                                      tGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGCGG‑GTCGCCCCGCCGGCGAttt                                     >  1:5171610/1‑92 (MQ=255)
                                                      tGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGACGTGGGATTTGTTTGTCTGCCGTTGCTGTCGGGG‑GTGGCCCAGGCGCCGGttt                                     >  1:3205719/1‑92 (MQ=255)
                                                       ggTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTat                                    <  2:1573228/93‑1 (MQ=255)
                                                       ggTGCTGAAGAACGGCCGCTTTGTAGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTat                                    >  2:2967024/1‑93 (MQ=255)
                                                        gTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTatc                                   <  1:1485951/93‑1 (MQ=255)
                                                        gTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTatc                                   <  1:5523906/93‑1 (MQ=255)
                                                            tGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGTGG‑GTCGCCCAGTCGCCGATTAtcatca                               >  2:635031/1‑93 (MQ=255)
                                                            tGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑GTCGCCCAGTCGCCGGTTTttatca                               >  2:2531427/1‑93 (MQ=255)
                                                            tGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCATTCGCCGATTAtcatca                               <  1:3919703/93‑1 (MQ=255)
                                                            tGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTAtcatca                               <  2:3629410/93‑1 (MQ=255)
                                                             gaagaaCGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATcatcat                              <  1:6012693/93‑1 (MQ=255)
                                                             gaagaaCGGCCGCTTTGTGGCGGGGGCGCCGGCGCCAGGATTCGTTAGTGTGCCCTTGCTGGCGTGGTTTCGCCC‑GTCCTCGAGAATcatcat                              >  2:5475813/1‑93 (MQ=255)
                                                               agaaCGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTa                            >  1:1988036/1‑93 (MQ=255)
                                                                gaaCGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTAc                           <  1:4774169/93‑1 (MQ=255)
                                                                gaaCGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCCGCCAGTCGCCGATTATTATCATTAc                           >  1:4950096/1‑93 (MQ=255)
                                                                  aCGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCg                         <  2:2666626/93‑1 (MQ=255)
                                                                  aCGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCg                         <  2:4573781/93‑1 (MQ=255)
                                                                  aCGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCg                         >  2:3045911/1‑93 (MQ=255)
                                                                    ggCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGAGTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGGCCAGTCGCCGGTTATTATCATTACCGGc                       >  2:1245833/1‑93 (MQ=255)
                                                                     gCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCGGCg                      >  2:2955999/1‑93 (MQ=255)
                                                                     gCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCAGCg                      >  2:3479913/1‑93 (MQ=255)
                                                                      ccGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGACGATTATCATCATTACCGGCGa                     <  1:4294570/93‑1 (MQ=255)
                                                                       cGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATTGCCCAGTCGCCGATTATCATCATTAACcgcgtg                    >  2:5549299/1‑91 (MQ=255)
                                                                       cGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCGGCGAg                    >  2:1391839/1‑93 (MQ=255)
                                                                       cGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCGGCGAg                    >  2:2007435/1‑93 (MQ=255)
                                                                        gCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑GTCGCCCAGTCGCCGATTATCATCATTACCGGCGGGc                   >  1:5002000/1‑93 (MQ=255)
                                                                        gCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGGCCAGTCGCCGGTTATTATCATTTACGGCgggg                   >  2:3444362/1‑90 (MQ=255)
                                                                         cTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCa                  >  1:2596493/1‑93 (MQ=255)
                                                                          tttGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑GTCGCCCCGTCGCCGAGTATCATCATTACCCGCGAGCAg                 >  1:2238565/1‑93 (MQ=255)
                                                                           ttGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGt                <  2:4860091/93‑1 (MQ=255)
                                                                               ggCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGag            <  2:2839573/93‑1 (MQ=255)
                                                                               ggCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGag            <  1:2515717/93‑1 (MQ=255)
                                                                               ggCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGag            >  1:333753/1‑93 (MQ=255)
                                                                                     ggcgccggcgctGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTa      <  1:942004/93‑1 (MQ=255)
                                                                                         ctggcgctggGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGGTGATCATCATTACTGGCGAGCAGTTGAGCAGCTATGaa  >  1:3625083/1‑93 (MQ=255)
                                                                                          |                                                                                            
CCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG‑ATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAA  >  NC_000913/883482‑883663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAAT  >  NC_000913/883481‑883664
                                                                                           |                                                                                            
CCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGG                                                                                            <  SRR4302537.3401714/94‑1 (MQ=60)
  CACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCG                                                                                          >  SRR4302537.1400150/1‑94 (MQ=60)
  CACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCG                                                                                          >  SRR4302537.4689366/1‑94 (MQ=60)
  CACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCG                                                                                          >  SRR4302537.3758920/1‑94 (MQ=60)
   ACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGC                                                                                         >  SRR4302537.6242594/1‑94 (MQ=60)
    CGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCT                                                                                        <  SRR4302537.3477871/94‑1 (MQ=60)
      CGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGG                                                                                      >  SRR4302537.1540175/1‑94 (MQ=60)
      CGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGG                                                                                      <  SRR4302537.3050446/94‑1 (MQ=60)
      CGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGG                                                                                      >  SRR4302537.1489542/1‑94 (MQ=60)
       GTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGG                                                                                     <  SRR4302537.4130651/94‑1 (MQ=60)
        TATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGGCGGCGCTGGGG                                                                                    >  SRR4302537.481745/1‑94 (MQ=60)
         ATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGAT                                                                                   >  SRR4302537.1830109/1‑94 (MQ=60)
          TAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATT                                                                                  <  SRR4302537.5466293/94‑1 (MQ=60)
             GCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGT                                                                               <  SRR4302537.2115660/94‑1 (MQ=60)
              CGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGGCGGCGCTGGGGTTCGTT                                                                              >  SRR4302537.4185863/1‑94 (MQ=60)
              CGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGGTTCGTT                                                                              >  SRR4302537.1905521/1‑94 (MQ=60)
                  AAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGATTCGTTAGTT                                                                          >  SRR4302537.1829398/1‑94 (MQ=60)
                  AAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTC                                                                          <  SRR4302537.2512211/94‑1 (MQ=60)
                    ACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGggttgttagtgtg                                                                        >  SRR4302537.1925747/1‑81 (MQ=60)
                     CTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGC                                                                       >  SRR4302537.3864012/1‑94 (MQ=60)
                         CACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGGTTCGTTAGTCTGCCGTT                                                                   >  SRR4302537.2986676/1‑94 (MQ=60)
                         CACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCCGGGGTTCGTTAGTTTGCCGTT                                                                   >  SRR4302537.3866162/1‑94 (MQ=60)
                          ACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGGCCGGGGCTGGGGTTCGTTAGTCTGCCGTTG                                                                  >  SRR4302537.1651411/1‑94 (MQ=60)
                          ACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGgtttgttagtttggcgttt                                                                  >  SRR4302537.102315/1‑75 (MQ=60)
                          ACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCCGCGCTGGGCTTCGTTAtgcttgcggtg                                                                  >  SRR4302537.881469/1‑83 (MQ=60)
                           CTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGC                                                                 <  SRR4302537.4675318/94‑1 (MQ=60)
                           CTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGC                                                                 >  SRR4302537.4315912/1‑94 (MQ=60)
                           CTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCCGGGATTCGTTAGTCTGCCGTTGC                                                                 >  SRR4302537.1081991/1‑94 (MQ=60)
                            TGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCT                                                                >  SRR4302537.4595552/1‑94 (MQ=60)
                            TGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCT                                                                >  SRR4302537.4999918/1‑94 (MQ=60)
                             GAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTG                                                               >  SRR4302537.3840280/1‑94 (MQ=60)
                              AAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTTCCGTTGCTGG                                                              >  SRR4302537.3880275/1‑94 (MQ=60)
                              AAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGG                                                              >  SRR4302537.792387/1‑94 (MQ=60)
                               AATAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGC                                                             <  SRR4302537.2586008/94‑1 (MQ=60)
                               AAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGGCCGCGCTGTGATTCGTTTGTCTGCCGCTGCTGGC                                                             >  SRR4302537.6113986/1‑94 (MQ=60)
                               AAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGC                                                             >  SRR4302537.6085563/1‑94 (MQ=60)
                                AGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTTGTCTGCCGTTGCTGGCG                                                            >  SRR4302537.3187843/1‑94 (MQ=60)
                                AGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCG                                                            <  SRR4302537.4841888/94‑1 (MQ=60)
                                AGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCG                                                            <  SRR4302537.4623063/94‑1 (MQ=60)
                                  AACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTG                                                          <  SRR4302537.4532414/94‑1 (MQ=60)
                                  AACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTG                                                          <  SRR4302537.4870929/94‑1 (MQ=60)
                                   ACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGG                                                         >  SRR4302537.1776901/1‑94 (MQ=60)
                                    CTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGA                                                        <  SRR4302537.2788140/94‑1 (MQ=60)
                                     TCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGAT                                                       <  SRR4302537.5236606/94‑1 (MQ=60)
                                      CGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATC                                                      <  SRR4302537.3391878/94‑1 (MQ=60)
                                         TCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCC                                                   >  SRR4302537.5945340/1‑94 (MQ=60)
                                          CGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGAGTCGTTAGTCTCGCGGTGCTGGCGTGGGTCGCCC                                                  >  SRR4302537.2293663/1‑94 (MQ=60)
                                            TGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCCG                                                >  SRR4302537.810590/1‑94 (MQ=60)
                                            TGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAG                                                <  SRR4302537.3606417/94‑1 (MQ=60)
                                            TGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAG                                                <  SRR4302537.2448476/94‑1 (MQ=60)
                                            TGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAG                                                <  SRR4302537.103504/94‑1 (MQ=60)
                                             GACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGggggtccgccagc                                               >  SRR4302537.4829034/1‑81 (MQ=60)
                                                  TAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCG                                          <  SRR4302537.531137/94‑1 (MQ=60)
                                                    AGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGAT                                        <  SRR4302537.5896060/94‑1 (MQ=60)
                                                      CTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGcgggtcgccccgccggcgattt                                      >  SRR4302537.5207217/1‑72 (MQ=60)
                                                      CTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGACGTGGGATTTGTTTGTCTGCCGTTGCTGtcgggggtggcccaggcgccggttt                                      >  SRR4302537.3228257/1‑69 (MQ=42)
                                                       TGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGCTTAT                                     >  SRR4302537.1974792/1‑94 (MQ=60)
                                                       TGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGGTGGCGTGGGTCGCCCAGTCGCCGGTTAT                                     >  SRR4302537.3972858/1‑94 (MQ=60)
                                                         GTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCA                                   <  SRR4302537.1496289/94‑1 (MQ=60)
                                                         GTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCA                                   <  SRR4302537.5561611/94‑1 (MQ=60)
                                                         GTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCA                                   <  SRR4302537.3683245/94‑1 (MQ=60)
                                                             TGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCATTCGCCGATTATCATCAT                               <  SRR4302537.3947099/94‑1 (MQ=60)
                                                             TGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCAT                               >  SRR4302537.6025650/1‑94 (MQ=60)
                                                             TGAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCCATTATCATCAT                               >  SRR4302537.4794516/1‑94 (MQ=60)
                                                              GAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATT                              <  SRR4302537.4223697/94‑1 (MQ=60)
                                                              GAAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATT                              <  SRR4302537.6054004/94‑1 (MQ=60)
                                                               AAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTA                             >  SRR4302537.5379029/1‑94 (MQ=60)
                                                               AAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTA                             >  SRR4302537.2001859/1‑94 (MQ=60)
                                                               AAGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTA                             <  SRR4302537.4548437/94‑1 (MQ=60)
                                                                AGAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCCGCCAGTCGCCGATTATTATCATTAC                            >  SRR4302537.4984236/1‑94 (MQ=60)
                                                                 GAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGGTAGTCTGCCGGTGCTGGCGTGGATCGCCCccgtgccgcgtatcatcattaac                           >  SRR4302537.6522474/1‑71 (MQ=60)
                                                                 GAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACC                           <  SRR4302537.4807129/94‑1 (MQ=60)
                                                                 GAACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACC                           <  SRR4302537.2206907/94‑1 (MQ=60)
                                                                  AACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGTTTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCG                          <  SRR4302537.4181877/94‑1 (MQ=60)
                                                                  AACGGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGGTTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCCATTATCATCATTTCCG                          >  SRR4302537.5648623/1‑94 (MQ=60)
                                                                     GGCCGCTTTGTGGCGGGGGGGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCG                       >  SRR4302537.5145884/1‑94 (MQ=60)
                                                                     GGCCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCG                       >  SRR4302537.4355101/1‑94 (MQ=60)
                                                                       CCGCTTTGTGGCGGGGGCGCCGGCGCTTGGATTCCTTTGTCTGCCGGTGCTGGCGTGGgttgcccacgcgccccgtttccttattagccgtgac                     >  SRR4302537.4775457/1‑58 (MQ=32)
                                                                       CCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAG                     <  SRR4302537.460310/94‑1 (MQ=60)
                                                                       CCGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGACGATTATCATCATTACCGGCGAG                     <  SRR4302537.4324428/94‑1 (MQ=60)
                                                                        CGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGGTCGCCCAGTCGCCGATTATCATCATTACCGGCGGGC                    >  SRR4302537.5036454/1‑94 (MQ=60)
                                                                        CGCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGC                    >  SRR4302537.3280386/1‑94 (MQ=60)
                                                                         GCTTTGTGGCGGGGGCGCTGGCGCTGGGGTTCGTTTGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCCATTATTATCATTACCcgcccgca                   >  SRR4302537.733814/1‑86 (MQ=60)
                                                                         GCTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCA                   >  SRR4302537.2614656/1‑94 (MQ=60)
                                                                          CTTTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGGTCGCCCCGTCGCCGAGTATCATCATTACCCGCGAGCAG                  >  SRR4302537.2254351/1‑94 (MQ=60)
                                                                            TTGTGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTT                <  SRR4302537.6220480/94‑1 (MQ=60)
                                                                               TGGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAG             >  SRR4302537.336063/1‑94 (MQ=60)
                                                                               TGGCAGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAG             <  SRR4302537.5071854/94‑1 (MQ=60)
                                                                                GGCGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGC            <  SRR4302537.2533395/94‑1 (MQ=60)
                                                                                  CGGGGGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAG          >  SRR4302537.4082454/1‑94 (MQ=60)
                                                                                      GGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTAT      <  SRR4302537.948501/94‑1 (MQ=60)
                                                                                      GGCGCCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTAT      <  SRR4302537.4395426/94‑1 (MQ=60)
                                                                                         GCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGGTGATCATCATTACTGGCGAGCAGTTGAGCAGCTATGAA   >  SRR4302537.3650630/1‑94 (MQ=60)
                                                                                          CCGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAAT  <  SRR4302537.1544184/94‑1 (MQ=60)
                                                                                           |                                                                                            
GCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAAT  >  NC_000913/883481‑883664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑