Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	924,932	A→G	intergenic (+169/+175)	clpA → / ← serW	ATP‑binding component of serine protease/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	924,932	0	A	G	93.7%	727.3 / 29.2	239	intergenic (+169/+175)	clpA/serW	ATP‑binding component of serine protease/tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base A (6/9); new base G (111/113); total (117/122)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

Only 100 of 243 total aligned reads displayed.

TTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTC  >  NC_000913/924841‑925021

ttCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGAt                                                                                          >  1:6345120/1‑93 (MQ=255)

 tCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTg                                                                                         <  2:5483337/93‑1 (MQ=255)

 tCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTg                                                                                         >  2:1236643/1‑93 (MQ=255)

 tCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTg                                                                                         <  1:4179304/93‑1 (MQ=255)

 tCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATg                                                                                         <  1:2928984/93‑1 (MQ=255)

   aGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGggtggt                                                                                       >  1:1198826/1‑93 (MQ=255)

    gACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAGGGTAAGGAAAAAAAAGGGGGCTGCGGGAGATTGGGGGGGtt                                                                                      >  2:4506221/1‑93 (MQ=255)

    gACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGtt                                                                                      >  2:4386652/1‑93 (MQ=255)

    gACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGtt                                                                                      >  1:4507468/1‑93 (MQ=255)

    gACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGtt                                                                                      >  1:1505995/1‑93 (MQ=255)

     aCCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGttt                                                                                     <  1:3107215/93‑1 (MQ=255)

       ccTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTg                                                                                   >  2:3073150/1‑93 (MQ=255)

       ccTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTg                                                                                   >  2:3032172/1‑93 (MQ=255)

         tttttttAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCt                                                                                 >  2:920669/1‑93 (MQ=255)

          ttttttAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCtt                                                                                >  1:4395466/1‑93 (MQ=255)

             tttAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTttat                                                                             <  2:2358467/93‑1 (MQ=255)

              ttAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTttatt                                                                            <  1:6268579/93‑1 (MQ=255)

               tAATGATGATGGTAAGTTTTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTttatta                                                                           <  1:2809140/93‑1 (MQ=255)

               tAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTttatta                                                                           <  2:3389603/93‑1 (MQ=255)

               tAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTttatta                                                                           <  2:2445142/93‑1 (MQ=255)

                 atgatgatgGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATTGGGTGGTTTTGCTTTATTaac                                                                         >  1:590988/1‑93 (MQ=255)

                 atgatgatgGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTaac                                                                         <  2:3471179/93‑1 (MQ=255)

                 atgatgatgGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTaac                                                                         <  1:3325049/93‑1 (MQ=255)

                 atgatgatgGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTaac                                                                         <  1:515711/93‑1 (MQ=255)

                  tgatgatgGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTaaca                                                                        <  1:6327930/93‑1 (MQ=255)

                  agatgatgGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTaaca                                                                        <  1:1947192/92‑1 (MQ=255)

                   gatgatgGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGATTTATTaacaa                                                                       >  1:610505/1‑93 (MQ=255)

                    atgatgGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTaacaac                                                                      >  1:507480/1‑93 (MQ=255)

                     tgatgGTAAGTTGTTGATAATTAGTGATGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACg                                                                     <  1:2215543/93‑1 (MQ=255)

                      gatgGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACgg                                                                    <  2:5602941/93‑1 (MQ=255)

                      gatgGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGAGGTTTTGCTTTATTAACAACgg                                                                    >  1:1078595/1‑93 (MQ=255)

                       atgGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACggg                                                                   >  1:4958182/1‑93 (MQ=255)

                        tgGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGc                                                                  <  2:1467205/93‑1 (MQ=255)

                          gTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGAGCTGCAGGAGAATGGGGTGGCTTTGCTTTATTAACAACGGGCTa                                                                >  1:1410943/1‑93 (MQ=255)

                            aaGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTaaa                                                              <  2:1161915/93‑1 (MQ=255)

                            aaGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTaaa                                                              <  1:1734334/93‑1 (MQ=255)

                             aGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGATaaag                                                             >  1:5648546/1‑92 (MQ=255)

                              gttgttGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACg                                                            >  2:1653208/1‑93 (MQ=255)

                              gttgttGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACg                                                            <  1:1381792/93‑1 (MQ=255)

                               ttgttgATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACgt                                                           >  2:425034/1‑93 (MQ=255)

                               ttgttgATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACgt                                                           >  1:3675094/1‑93 (MQ=255)

                               ttgttgATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACgt                                                           >  1:861858/1‑93 (MQ=255)

                                  ttgATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGCATGGGGTGGTTTTGCTTTATTAACAACGCGCTAAACGTgta                                                        >  1:4813037/1‑93 (MQ=255)

                                  ttgATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTgta                                                        >  2:2657250/1‑93 (MQ=255)

                                  ttgATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTgta                                                        <  1:3919398/93‑1 (MQ=255)

                                   tgATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTgtag                                                       >  2:4535040/1‑93 (MQ=255)

                                    gATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTgtagt                                                      >  2:2872118/1‑93 (MQ=255)

                                     aTAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTgtagta                                                     <  2:5535075/93‑1 (MQ=255)

                                     aTAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTgtagta                                                     <  2:508071/93‑1 (MQ=255)

                                       aaTTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTAtt                                                   >  1:3856352/1‑93 (MQ=255)

                                              gctgcGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTc                                            <  2:6097044/93‑1 (MQ=255)

                                                 gcGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACt                                         <  1:5527442/93‑1 (MQ=255)

                                                 gcGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACt                                         <  2:807024/93‑1 (MQ=255)

                                                 gcGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACt                                         <  1:2386736/93‑1 (MQ=255)

                                                  cGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTg                                        <  1:663076/93‑1 (MQ=255)

                                                   gggAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGc                                       >  2:5370101/1‑93 (MQ=255)

                                                        ggTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTAc                                  >  2:4114214/1‑93 (MQ=255)

                                                         gTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACa                                 >  2:4820025/1‑93 (MQ=255)

                                                          tAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAg                                <  2:161683/93‑1 (MQ=255)

                                                            aGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGc                              >  2:389204/1‑93 (MQ=255)

                                                            aGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGc                              >  1:2788220/1‑93 (MQ=255)

                                                            aGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGc                              <  2:1973708/93‑1 (MQ=255)

                                                             ggATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCa                             <  1:1471697/93‑1 (MQ=255)

                                                             ggATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCa                             <  1:3802218/93‑1 (MQ=255)

                                                             ggATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCa                             >  1:1615352/1‑93 (MQ=255)

                                                             ggATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCa                             >  2:5300239/1‑93 (MQ=255)

                                                             ggATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCa                             >  2:6396637/1‑93 (MQ=255)

                                                             ggATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCa                             <  1:300346/93‑1 (MQ=255)

                                                               aTAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGc                           <  1:2332286/93‑1 (MQ=255)

                                                                tAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCt                          <  1:2476391/93‑1 (MQ=255)

                                                                 aaaaaaGTGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCtt                         <  1:1112863/93‑1 (MQ=255)

                                                                  aaaaaGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTa                        <  1:3788242/93‑1 (MQ=255)

                                                                  aaaaaGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTa                        <  2:2777508/93‑1 (MQ=255)

                                                                  aaaaaGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTa                        <  1:5599305/93‑1 (MQ=255)

                                                                   aaaaGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAg                       <  1:1267799/93‑1 (MQ=255)

                                                                      aGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGaaa                    >  2:1765091/1‑93 (MQ=255)

                                                                      aGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGaaa                    >  2:6072588/1‑93 (MQ=255)

                                                                      aGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGaaa                    <  1:959931/93‑1 (MQ=255)

                                                                      aGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGaaa                    <  1:5244711/93‑1 (MQ=255)

                                                                       gggTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAAt                   <  1:6152855/93‑1 (MQ=255)

                                                                       gggTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAAt                   >  1:4591520/1‑93 (MQ=255)

                                                                       gggTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAAt                   >  1:2559905/1‑93 (MQ=255)

                                                                           gctgcAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTcac               <  2:3696985/93‑1 (MQ=255)

                                                                               cAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGt           >  2:5759308/1‑93 (MQ=255)

                                                                                aGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTa          <  2:4237988/93‑1 (MQ=255)

                                                                                aGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTa          <  1:1450024/93‑1 (MQ=255)

                                                                                 ggggaATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAAAGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTac         >  2:5939368/4‑92 (MQ=255)

                                                                                 ggAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTaa         <  2:6353869/93‑1 (MQ=255)

                                                                                 ggAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTaa         <  1:1786210/93‑1 (MQ=255)

                                                                                  gagaATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAAc        <  2:4969743/93‑1 (MQ=255)

                                                                                   agaATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACa       >  2:2498405/1‑93 (MQ=255)

                                                                                   agaATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACa       <  1:2678913/93‑1 (MQ=255)

                                                                                    gaATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACAt      <  1:1017517/93‑1 (MQ=255)

                                                                                    gaATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACAt      >  1:3272785/1‑93 (MQ=255)

                                                                                    gaATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACAt      >  1:4694894/1‑93 (MQ=255)

                                                                                    gaAGGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACAt      <  1:4255285/93‑1 (MQ=255)

                                                                                     aaTGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATa     <  2:312156/93‑1 (MQ=255)

                                                                                       tGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACt   >  2:4330320/1‑93 (MQ=255)

                                                                                       tGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACt   <  2:1521633/93‑1 (MQ=255)

                                                                                        ggggTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTc  >  1:2936011/1‑93 (MQ=255)

TTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTC  >  NC_000913/924841‑925021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACC  >  NC_000913/924839‑925025

GGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGG                                                                                               >  SRR4302537.5136377/1‑94 (MQ=60)

 GTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGAT                                                                                              >  SRR4302537.6388459/1‑94 (MQ=60)

  TTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTG                                                                                             <  SRR4302537.5225084/94‑1 (MQ=60)

  TTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTG                                                                                             <  SRR4302537.5167080/94‑1 (MQ=60)

  TTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTG                                                                                             >  SRR4302537.5090347/1‑94 (MQ=60)

  TTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTG                                                                                             <  SRR4302537.3974414/94‑1 (MQ=60)

  TTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTG                                                                                             <  SRR4302537.2063949/94‑1 (MQ=60)

  TTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTG                                                                                             <  SRR4302537.185994/94‑1 (MQ=60)

  TTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGATGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTG                                                                                             <  SRR4302537.3568667/94‑1 (MQ=60)

   TCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGG                                                                                            >  SRR4302537.911226/1‑94 (MQ=60)

   TCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGG                                                                                            <  SRR4302537.4208476/94‑1 (MQ=60)

   TCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGG                                                                                            <  SRR4302537.2949553/94‑1 (MQ=60)

    CAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGT                                                                                           >  SRR4302537.1207158/1‑94 (MQ=60)

     AGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTT                                                                                          >  SRR4302537.1794658/1‑94 (MQ=60)

     AGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTT                                                                                          >  SRR4302537.4538636/1‑94 (MQ=60)

     AGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTT                                                                                          >  SRR4302537.1516502/1‑94 (MQ=60)

       ACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTT                                                                                        <  SRR4302537.3128916/94‑1 (MQ=60)

        CCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTG                                                                                       >  SRR4302537.517078/1‑94 (MQ=60)

         CCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGC                                                                                      >  SRR4302537.5596947/1‑94 (MQ=60)

          CTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCT                                                                                     <  SRR4302537.2074218/94‑1 (MQ=60)

          CTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCT                                                                                     >  SRR4302537.1346835/1‑94 (MQ=60)

           TTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTT                                                                                    >  SRR4302537.3872660/1‑94 (MQ=60)

           TTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTT                                                                                    >  SRR4302537.4425789/1‑94 (MQ=60)

            TTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTT                                                                                   <  SRR4302537.1162722/94‑1 (MQ=60)

            TTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTT                                                                                   >  SRR4302537.1588242/1‑94 (MQ=60)

              TTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTAT                                                                                 <  SRR4302537.3337299/94‑1 (MQ=60)

               TTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATT                                                                                <  SRR4302537.3903716/94‑1 (MQ=60)

                TTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTA                                                                               <  SRR4302537.6311602/94‑1 (MQ=60)

                TTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTA                                                                               <  SRR4302537.6311604/94‑1 (MQ=60)

                 TAATGATGATGGTAAGTTTTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAA                                                                              <  SRR4302537.2828778/94‑1 (MQ=60)

                 TAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAA                                                                              <  SRR4302537.2376687/94‑1 (MQ=60)

                  AATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAACAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAAC                                                                             >  SRR4302537.2818198/1‑94 (MQ=60)

                  AATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATTGGGTGGTTTTGCTTTATTAAC                                                                             >  SRR4302537.595031/1‑94 (MQ=60)

                  AATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAAC                                                                             <  SRR4302537.5263942/94‑1 (MQ=60)

                   ATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGTTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACA                                                                            <  SRR4302537.2826385/94‑1 (MQ=60)

                   ATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACA                                                                            <  SRR4302537.519260/94‑1 (MQ=60)

                   ATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACA                                                                            <  SRR4302537.3348576/94‑1 (MQ=60)

                    TGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAA                                                                           <  SRR4302537.2594618/94‑1 (MQ=60)

                    TGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAA                                                                           <  SRR4302537.6371207/94‑1 (MQ=60)

                    TGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAA                                                                           >  SRR4302537.5716082/1‑94 (MQ=60)

                    TGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGATTTATTAACAA                                                                           >  SRR4302537.614676/1‑94 (MQ=60)

                    AGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAA                                                                           <  SRR4302537.1960732/94‑1 (MQ=60)

                     GATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAAC                                                                          >  SRR4302537.510964/1‑94 (MQ=60)

                      ATGATGGTAAGTTGTTGATAATTAGTGCTGCTGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACC                                                                         <  SRR4302537.2224117/94‑1 (MQ=60)

                       TGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGG                                                                        >  SRR4302537.2956211/1‑94 (MQ=60)

                       TGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGG                                                                        >  SRR4302537.404218/1‑94 (MQ=60)

                       TGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGG                                                                        <  SRR4302537.108958/94‑1 (MQ=60)

                       TGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGAGGTTTTGCTTTATTAACAACGG                                                                        >  SRR4302537.1086035/1‑94 (MQ=60)

                       TGATGGTAAGTTGTTGATAATTAGTGATGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGG                                                                        <  SRR4302537.2231165/94‑1 (MQ=60)

                        GATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGG                                                                       >  SRR4302537.4992363/1‑94 (MQ=60)

                           GGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGAGCTGCAGGAGAATGGGGTGGCTTTGCTTTATTAACAACGGGCTA                                                                    >  SRR4302537.1420771/1‑94 (MQ=60)

                              AAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAAC                                                                 <  SRR4302537.1746430/94‑1 (MQ=60)

                              AAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAAC                                                                 <  SRR4302537.1515035/94‑1 (MQ=60)

                              AAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAAC                                                                 <  SRR4302537.1444566/94‑1 (MQ=60)

                              AAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGATAAAG                                                                 >  SRR4302537.5687205/1‑94 (MQ=60)

                              AAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATCAACAACGGGCTAAAC                                                                 <  SRR4302537.3271100/94‑1 (MQ=60)

                                GTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGT                                                               >  SRR4302537.867765/1‑94 (MQ=60)

                                GTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGT                                                               >  SRR4302537.3432032/1‑94 (MQ=60)

                                GTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGT                                                               >  SRR4302537.3700943/1‑94 (MQ=60)

                                GTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACGT                                                               <  SRR4302537.1391433/94‑1 (MQ=60)

                                 TTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTG                                                              >  SRR4302537.5935499/1‑94 (MQ=60)

                                  TGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGT                                                             >  SRR4302537.5991310/1‑94 (MQ=60)

                                   GTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGCATGGGGTGGTTTTGCTTTATTAACAACGCGCTAAACGTGTA                                                            >  SRR4302537.4846261/1‑94 (MQ=60)

                                    TTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAG                                                           <  SRR4302537.3946794/94‑1 (MQ=60)

                                    TTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAG                                                           <  SRR4302537.4726015/94‑1 (MQ=60)

                                     TGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGT                                                          >  SRR4302537.122701/1‑94 (MQ=60)

                                       ATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTAT                                                        <  SRR4302537.6275199/94‑1 (MQ=60)

                                       ATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTAT                                                        <  SRR4302537.490468/94‑1 (MQ=60)

                                        TAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATT                                                       >  SRR4302537.1076010/1‑94 (MQ=60)

                                        TAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATT                                                       >  SRR4302537.3883343/1‑94 (MQ=60)

                                         AATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTT                                                      >  SRR4302537.778737/1‑94 (MQ=60)

                                          ATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTG                                                     >  SRR4302537.2558072/1‑94 (MQ=60)

                                          ATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTG                                                     <  SRR4302537.4950410/94‑1 (MQ=60)

                                          ATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTG                                                     <  SRR4302537.4654384/94‑1 (MQ=60)

                                             AGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGT                                                  >  SRR4302537.6496532/1‑94 (MQ=60)

                                              GTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTT                                                 <  SRR4302537.1406618/94‑1 (MQ=60)

                                                  TGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACT                                             <  SRR4302537.778115/94‑1 (MQ=60)

                                                   GCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTG                                            <  SRR4302537.4553405/94‑1 (MQ=60)

                                                   GCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTG                                            <  SRR4302537.5565175/94‑1 (MQ=60)

                                                   GCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTG                                            <  SRR4302537.2403546/94‑1 (MQ=60)

                                                    CGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGC                                           <  SRR4302537.667590/94‑1 (MQ=60)

                                                     GGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCC                                          <  SRR4302537.1463951/94‑1 (MQ=60)

                                                        AAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTA                                       <  SRR4302537.3586568/94‑1 (MQ=60)

                                                         AGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTAC                                      >  SRR4302537.4227319/1‑94 (MQ=60)

                                                         AGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTAC                                      >  SRR4302537.562751/1‑94 (MQ=60)

                                                           GTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAG                                    <  SRR4302537.5446625/94‑1 (MQ=60)

                                                             AAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGC                                  >  SRR4302537.2807715/1‑94 (MQ=60)

                                                             AAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGC                                  >  SRR4302537.1657152/1‑94 (MQ=60)

                                                             AAGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGC                                  >  SRR4302537.4288278/1‑94 (MQ=60)

                                                              AGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCA                                 >  SRR4302537.1626637/1‑94 (MQ=60)

                                                              AGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCA                                 <  SRR4302537.2957121/94‑1 (MQ=60)

                                                              AGGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCA                                 >  SRR4302537.4792859/1‑94 (MQ=60)

                                                               GGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAG                                <  SRR4302537.302440/94‑1 (MQ=60)

                                                               GGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAG                                <  SRR4302537.1481928/94‑1 (MQ=60)

                                                               GGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAG                                <  SRR4302537.3828889/94‑1 (MQ=60)

                                                               GGATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAG                                >  SRR4302537.1323548/1‑94 (MQ=60)

                                                                GATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGC                               >  SRR4302537.2784240/1‑94 (MQ=60)

                                                                 ATAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCT                              <  SRR4302537.2348728/94‑1 (MQ=60)

                                                                  TAAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTT                             <  SRR4302537.2493800/94‑1 (MQ=60)

                                                                   AAAAAAGTGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTA                            <  SRR4302537.1120547/94‑1 (MQ=60)

                                                                   AAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTA                            >  SRR4302537.1789524/1‑94 (MQ=60)

                                                                   AAAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTA                            <  SRR4302537.5136604/94‑1 (MQ=60)

                                                                    AAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAG                           <  SRR4302537.5637585/94‑1 (MQ=60)

                                                                    AAAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAG                           <  SRR4302537.3814836/94‑1 (MQ=60)

                                                                     AAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGA                          >  SRR4302537.2481225/1‑94 (MQ=60)

                                                                     AAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGA                          >  SRR4302537.2657449/1‑94 (MQ=60)

                                                                     AAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGA                          <  SRR4302537.1276617/94‑1 (MQ=60)

                                                                     AAAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGA                          >  SRR4302537.3231024/1‑94 (MQ=60)

                                                                      AAAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAA                         >  SRR4302537.1952088/1‑94 (MQ=60)

                                                                       AAGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAA                        >  SRR4302537.1529407/1‑94 (MQ=60)

                                                                        AGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAAT                       <  SRR4302537.5280801/94‑1 (MQ=60)

                                                                        AGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAAT                       <  SRR4302537.966550/94‑1 (MQ=60)

                                                                        AGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAAT                       >  SRR4302537.4623282/1‑94 (MQ=60)

                                                                        AGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAAT                       >  SRR4302537.2577845/1‑94 (MQ=60)

                                                                        AGGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAAT                       >  SRR4302537.3110558/1‑94 (MQ=60)

                                                                         GGGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATT                      <  SRR4302537.6195182/94‑1 (MQ=60)

                                                                         GGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATT                      <  SRR4302537.1437474/94‑1 (MQ=60)

                                                                          GGTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTC                     >  SRR4302537.2424079/1‑94 (MQ=60)

                                                                           GTGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCA                    <  SRR4302537.3869781/94‑1 (MQ=60)

                                                                            TGCTGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCAC                   >  SRR4302537.4514725/1‑94 (MQ=60)

                                                                               TGCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGG                <  SRR4302537.1201267/94‑1 (MQ=60)

                                                                                GCAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGT               <  SRR4302537.4070336/94‑1 (MQ=60)

                                                                                 CAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTA              <  SRR4302537.6378638/94‑1 (MQ=60)

                                                                                 CAGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTA              <  SRR4302537.6167324/94‑1 (MQ=60)

                                                                                  AGGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAA             <  SRR4302537.1460127/94‑1 (MQ=60)

                                                                                   GGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAAC            <  SRR4302537.5663197/94‑1 (MQ=60)

                                                                                   GGAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAAC            <  SRR4302537.1798668/94‑1 (MQ=60)

                                                                                    GAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACA           >  SRR4302537.2246382/1‑94 (MQ=60)

                                                                                    GAGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACA           >  SRR4302537.3827319/1‑94 (MQ=60)

                                                                                     AGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACAT          >  SRR4302537.2976900/1‑94 (MQ=60)

                                                                                     AGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACAT          >  SRR4302537.4727369/1‑94 (MQ=60)

                                                                                     AGAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACAT          >  SRR4302537.3295882/1‑94 (MQ=60)

                                                                                     AGAATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACAT          <  SRR4302537.2697618/94‑1 (MQ=60)

                                                                                      GAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATA         <  SRR4302537.1024522/94‑1 (MQ=60)

                                                                                      GAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATA         >  SRR4302537.6380490/1‑94 (MQ=60)

                                                                                      GAATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATA         <  SRR4302537.4351155/94‑1 (MQ=60)

                                                                                      GAAGGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATA         <  SRR4302537.4284861/94‑1 (MQ=60)

                                                                                       AATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATAC        >  SRR4302537.6193950/1‑94 (MQ=60)

                                                                                       AATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATAC        >  SRR4302537.2859468/1‑94 (MQ=60)

                                                                                       AATGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATAC        >  SRR4302537.3718854/1‑94 (MQ=60)

                                                                                         TGGGGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTC      >  SRR4302537.2956627/1‑94 (MQ=60)

                                                                                            GGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCAC   <  SRR4302537.4047252/94‑1 (MQ=60)

                                                                                            GGTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCAC   <  SRR4302537.1010463/94‑1 (MQ=60)

                                                                                             GTGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACC  >  SRR4302537.5552611/1‑94 (MQ=60)

GGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGCTAAACGTGTAGTATTTGAGTTCACTGCCGTACAGGCAGCTTAGAAATTCACAGGTAACATACTCCACC  >  NC_000913/924839‑925025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`