Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 926,255 A→G V134A (GTC→GCC)  aat ← leucyl, phenylalanyl‑tRNA‑protein transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913926,2550AG98.9% 913.7 / ‑1.4 267V134A (GTC→GCC) aatleucyl, phenylalanyl‑tRNA‑protein transferase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (145/119);  total (146/121)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 284 total aligned reads displayed.

CATTTTCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGACTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGC  >  NC_000913/926163‑926345
                                                                                            |                                                                                          
   tttCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTc                                                                                        <  2:8147933/94‑1 (MQ=255)
   tttCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTc                                                                                        <  2:7041117/94‑1 (MQ=255)
    ttCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCa                                                                                       >  2:6876049/1‑94 (MQ=255)
    ttCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCGTCACGCCAGGCTTCa                                                                                       >  1:3702940/1‑94 (MQ=255)
      ccATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAAt                                                                                     <  1:5009603/94‑1 (MQ=255)
      ccATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAAt                                                                                     <  2:7897324/94‑1 (MQ=255)
       cATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATg                                                                                    >  1:934295/1‑94 (MQ=255)
        aTCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTTATCTTCACGTCAGTCTTCAATgg                                                                                   >  1:2641463/1‑94 (MQ=255)
        aTCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATgg                                                                                   >  1:5483314/1‑94 (MQ=255)
         tCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGa                                                                                  >  1:9257664/1‑94 (MQ=255)
           cGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGt                                                                                <  1:614449/94‑1 (MQ=255)
            ggCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTg                                                                               >  1:976694/1‑94 (MQ=255)
             gCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTgg                                                                              <  2:10361255/94‑1 (MQ=255)
               tGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATCCCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGc                                                                            >  1:6454923/1‑94 (MQ=255)
                gAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCa                                                                           >  2:5611564/1‑94 (MQ=255)
                 aaCATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCAt                                                                          <  2:3134092/94‑1 (MQ=255)
                  aCATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATg                                                                         >  2:8812266/1‑94 (MQ=255)
                  aCATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATC‑TCACGCCAGGCTTCCATGGAGGGGGCATgg                                                                        >  1:6532677/1‑94 (MQ=255)
                   cATGGACTCGCCACAAAATAGCGTTCCCTGGGCCCCGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATgg                                                                        <  1:8084570/94‑1 (MQ=255)
                    aTGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGc                                                                       <  2:3796561/94‑1 (MQ=255)
                    aTGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGc                                                                       <  2:5856578/94‑1 (MQ=255)
                     tGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGcc                                                                      <  2:6870519/94‑1 (MQ=255)
                     tGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGcc                                                                      <  2:8691966/94‑1 (MQ=255)
                        aCTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAg                                                                   >  1:10295392/1‑94 (MQ=255)
                         cTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGt                                                                  >  1:8394611/1‑94 (MQ=255)
                         cTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGt                                                                  >  1:3906396/1‑94 (MQ=255)
                          tCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGtt                                                                 >  1:10443701/1‑94 (MQ=255)
                          tCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGtt                                                                 <  2:10086288/94‑1 (MQ=255)
                            gCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCg                                                               <  2:5323205/94‑1 (MQ=255)
                            gCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCg                                                               >  1:2865889/1‑94 (MQ=255)
                             ccACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGt                                                              >  2:6258522/1‑94 (MQ=255)
                             ccACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGt                                                              >  2:3908626/1‑94 (MQ=255)
                              cacaAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATTCAGCGCGCACGGCCGTGTTCGTg                                                             >  1:6809629/1‑94 (MQ=255)
                              cacaAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTg                                                             >  2:8481053/1‑94 (MQ=255)
                               acaAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGa                                                            >  1:7723518/1‑94 (MQ=255)
                                 aaaaTAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAg                                                          >  2:446420/1‑94 (MQ=255)
                                   aaTAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGc                                                        <  2:1099874/94‑1 (MQ=255)
                                   aaTAGCGTTCCCTGGGCCACGCCATACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGc                                                        <  1:5812093/94‑1 (MQ=255)
                                    aTAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCg                                                       >  1:2227168/1‑94 (MQ=255)
                                    aTAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGATCGTGAAGGCg                                                       >  2:7253423/1‑94 (MQ=255)
                                      aGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGAt                                                     <  2:3432917/94‑1 (MQ=255)
                                      aGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGAt                                                     <  1:4709594/94‑1 (MQ=255)
                                            cccTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAgg                                               <  1:1596530/94‑1 (MQ=255)
                                            cccTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAgg                                               <  2:7995329/94‑1 (MQ=255)
                                            cccTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAgg                                               <  1:5446736/94‑1 (MQ=255)
                                             ccTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGc                                              >  2:2380460/1‑94 (MQ=255)
                                              cTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCt                                             >  2:2705706/1‑94 (MQ=255)
                                               tGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGTCCGAGTTCGTGAAGGCGATGGTAGGCtt                                            >  1:45555/1‑94 (MQ=255)
                                               tGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCtt                                            >  2:9924722/1‑94 (MQ=255)
                                               tGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCtt                                            <  1:5571557/94‑1 (MQ=255)
                                                 ggCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCg                                          >  2:10026060/1‑94 (MQ=255)
                                                 ggCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCg                                          >  1:1911192/1‑94 (MQ=255)
                                                 ggCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCg                                          <  2:6530339/94‑1 (MQ=255)
                                                   ccACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAc                                        >  1:4034627/1‑94 (MQ=255)
                                                    cACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAcc                                       >  1:5251/1‑94 (MQ=255)
                                                    cACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAcc                                       >  2:199966/1‑94 (MQ=255)
                                                    cACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAcc                                       <  1:8903761/94‑1 (MQ=255)
                                                     aCGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAcca                                      >  2:2042394/1‑94 (MQ=255)
                                                     aCGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAcca                                      >  1:1245092/1‑94 (MQ=255)
                                                     aCGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAcca                                      >  2:6135205/1‑94 (MQ=255)
                                                       gCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAccacg                                    <  2:4575079/94‑1 (MQ=255)
                                                         cGTACATCCCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAccacgcc                                  <  1:3424661/94‑1 (MQ=255)
                                                         cGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAccacgcc                                  >  1:2700193/1‑94 (MQ=255)
                                                          gTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAccacgcca                                 >  1:1141967/1‑94 (MQ=255)
                                                          gTACATACCGCCGACAAGCTCATCATCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAccacgcca                                 <  2:3445296/94‑1 (MQ=255)
                                                           tACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAccacgccac                                <  2:2510266/94‑1 (MQ=255)
                                                            aCATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTCGGCTTCGAccacgccacg                               >  1:4872143/1‑94 (MQ=255)
                                                             cATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACcacgccacgc                              <  1:7392413/94‑1 (MQ=255)
                                                              aTACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCCCGCCAcgcg                             <  1:3625083/94‑1 (MQ=255)
                                                              aTACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCAcgcg                             >  1:10587653/1‑94 (MQ=255)
                                                                 ccgccgACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGa                          <  1:746225/94‑1 (MQ=255)
                                                                   gccgACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATc                        >  1:2431504/1‑94 (MQ=255)
                                                                    ccgACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATcc                       >  1:8008424/1‑94 (MQ=255)
                                                                    ccgACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATcc                       >  2:8983125/1‑94 (MQ=255)
                                                                      gACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAg                     >  2:1465356/1‑94 (MQ=255)
                                                                      gACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAg                     >  2:915213/1‑94 (MQ=255)
                                                                       aCAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAgg                    >  2:7478953/1‑94 (MQ=255)
                                                                        cAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGt                   <  1:9017304/94‑1 (MQ=255)
                                                                          aGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTc                 <  1:6741201/94‑1 (MQ=255)
                                                                           gCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTcc                >  1:7137699/1‑94 (MQ=255)
                                                                            cTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCct               >  2:9573952/1‑94 (MQ=255)
                                                                             tCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGCTCCAGGTTCctt              >  1:938785/1‑94 (MQ=255)
                                                                             tCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCctt              <  1:4308891/94‑1 (MQ=255)
                                                                             tCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCctt              >  2:4050332/1‑94 (MQ=255)
                                                                               aTCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCcttct            <  2:2953329/94‑1 (MQ=255)
                                                                               aTCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGGTCGTGAAGGCGCTGGTAGGCTTCGACCACGCCACGCGTGATCCCGGTTCcttct            >  2:4311015/1‑94 (MQ=255)
                                                                               aTCTTCACGCCAGGCTACAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCcttct            <  2:9420239/94‑1 (MQ=255)
                                                                                 cctCACGCCAGGCGTCAAGGGATGTGTCATGGCCGAGTTCGGAAAGGCGATGGTAGGCGTCGACCACGCCACGCGTGATCCAGGTTCCttcttc          <  1:10147677/92‑1 (MQ=255)
                                                                                 cTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCttcttc          <  2:4216145/94‑1 (MQ=255)
                                                                                 cTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCttcttc          <  2:7197077/94‑1 (MQ=255)
                                                                                  ttCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTcg         <  2:7684027/94‑1 (MQ=255)
                                                                                  ttCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTcg         >  2:4995316/1‑94 (MQ=255)
                                                                                  ttCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTcg         <  2:921931/94‑1 (MQ=255)
                                                                                    cACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTcgcg       >  2:6655867/1‑94 (MQ=255)
                                                                                    cACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTcgcg       >  1:8784820/1‑94 (MQ=255)
                                                                                    cACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTcgcg       >  1:2018737/1‑94 (MQ=255)
                                                                                    cACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTcgcg       <  1:7910162/94‑1 (MQ=255)
                                                                                      cGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGAt     >  1:9872820/1‑94 (MQ=255)
                                                                                       gCCAGGCTTCAATGGAGGGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATc    <  2:3270886/94‑1 (MQ=255)
                                                                                        ccAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCg   >  2:5932915/1‑94 (MQ=255)
                                                                                            |                                                                                          
CATTTTCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGACTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGC  >  NC_000913/926163‑926345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCATTTTCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGACTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTG  >  NC_000913/926162‑926347
                                                                                             |                                                                                            
GCATTTTCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGG                                                                                              >  SRR4302545.9333273/1‑94 (MQ=60)
 CATTTTCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGC                                                                                             >  SRR4302545.8251192/1‑94 (MQ=60)
 CATTTTCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGAC                                                                                             <  SRR4302545.3738140/94‑1 (MQ=60)
  ATTTTCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCT                                                                                            <  SRR4302545.8538409/94‑1 (MQ=60)
  ATTTTCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCT                                                                                            <  SRR4302545.5799034/94‑1 (MQ=60)
     TTCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCGCGCCAGGCTTCA                                                                                         <  SRR4302545.2145059/94‑1 (MQ=60)
     TTCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCA                                                                                         >  SRR4302545.3793737/1‑94 (MQ=60)
     TTCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCGTCACGCCAGGCTTCA                                                                                         >  SRR4302545.3793740/1‑94 (MQ=60)
      TCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAA                                                                                        >  SRR4302545.7361932/1‑94 (MQ=60)
      TCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACAGCCGACAAGCTCATCTTCACGCCAGGCTTCAA                                                                                        >  SRR4302545.952402/1‑94 (MQ=60)
       CCATCCGGCTGAACATGGACTCGGCACAAAATTGGCTTCCCTGGGCCACGGCGTTCAATCCGCCGACAAGCTCATCTTCACGCCAGGCTTCAAT                                                                                       <  SRR4302545.10185627/94‑1 (MQ=53)
       CCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAAT                                                                                       <  SRR4302545.5136618/94‑1 (MQ=60)
       CCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAAT                                                                                       <  SRR4302545.5136617/94‑1 (MQ=60)
        CATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATG                                                                                      >  SRR4302545.955250/1‑94 (MQ=60)
         ATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTTATCTTCACGTCAGTCTTCAATGG                                                                                     >  SRR4302545.2705033/1‑94 (MQ=60)
         ATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGG                                                                                     <  SRR4302545.10766124/94‑1 (MQ=60)
         ATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGG                                                                                     >  SRR4302545.2705034/1‑94 (MQ=60)
         ATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGG                                                                                     >  SRR4302545.5627392/1‑94 (MQ=60)
          TCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGA                                                                                    <  SRR4302545.3641293/94‑1 (MQ=60)
          TCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGA                                                                                    >  SRR4302545.9529086/1‑94 (MQ=60)
            CGGCTGAACATGGACTCGTCACAAAATAGCGTTGCCTGGGCCACGCCGTACATACTGACGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGG                                                                                  >  SRR4302545.1244049/1‑94 (MQ=60)
            CGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGT                                                                                  <  SRR4302545.628263/94‑1 (MQ=60)
             GGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTG                                                                                 <  SRR4302545.1990805/94‑1 (MQ=60)
             GGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTG                                                                                 <  SRR4302545.5417847/94‑1 (MQ=60)
             GGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTG                                                                                 >  SRR4302545.998503/1‑94 (MQ=60)
              GCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGG                                                                                >  SRR4302545.245171/1‑94 (MQ=60)
              GCTGAAAATGGACGCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGG                                                                                <  SRR4302545.2505675/94‑1 (MQ=60)
              GCTCACCATGGACTCGCCACAAAATAGCGTGCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGG                                                                                <  SRR4302545.1543773/94‑1 (MQ=60)
                TGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATCCCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGC                                                                              >  SRR4302545.6631397/1‑94 (MQ=60)
                TGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGC                                                                              <  SRR4302545.8095357/94‑1 (MQ=60)
                 GAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCA                                                                             >  SRR4302545.762409/1‑94 (MQ=60)
                  AACATGGCCTCGCCACAAAATAGCGTTCCCTGGGCCAGGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAAGGGAGTGGGCAT                                                                            <  SRR4302545.10923137/94‑1 (MQ=60)
                   ACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATG                                                                           <  SRR4302545.1153527/94‑1 (MQ=60)
                   ACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATC‑TCACGCCAGGCTTCCATGGAGGGGGCATGG                                                                          >  SRR4302545.6711387/1‑94 (MQ=60)
                    CATGGACTCGCCACAAAATAGCGTTCCCTGGGCCCCGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGG                                                                          <  SRR4302545.8317022/94‑1 (MQ=60)
                    CATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGG                                                                          <  SRR4302545.6671856/94‑1 (MQ=60)
                    CATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGG                                                                          <  SRR4302545.6671855/94‑1 (MQ=60)
                    CATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGG                                                                          <  SRR4302545.3306197/94‑1 (MQ=60)
                    CATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGG                                                                          <  SRR4302545.9352642/94‑1 (MQ=60)
                     ATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGC                                                                         >  SRR4302545.3990210/1‑94 (MQ=60)
                         ACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAG                                                                     >  SRR4302545.10599010/1‑94 (MQ=60)
                          CTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGT                                                                    >  SRR4302545.4003890/1‑94 (MQ=60)
                          CTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGT                                                                    >  SRR4302545.8637583/1‑94 (MQ=60)
                           TCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTT                                                                   >  SRR4302545.10751434/1‑94 (MQ=60)
                            CGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTC                                                                  <  SRR4302545.7221484/94‑1 (MQ=60)
                            CGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGACTTCAATGGAGTGGGCATGGCCGAGTTC                                                                  <  SRR4302545.9159473/94‑1 (MQ=60)
                             GCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCG                                                                 >  SRR4302545.2935174/1‑94 (MQ=60)
                             GCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCG                                                                 <  SRR4302545.3666283/94‑1 (MQ=60)
                               CACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATtcagcgcgcacggccgtgttcgtg                                                               >  SRR4302545.6996767/1‑70 (MQ=60)
                               CACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTG                                                               >  SRR4302545.6996765/1‑94 (MQ=60)
                               CACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTG                                                               >  SRR4302545.6996766/1‑94 (MQ=60)
                               CACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTG                                                               >  SRR4302545.5616983/1‑94 (MQ=60)
                               CACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCAAGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTG                                                               >  SRR4302545.4214303/1‑94 (MQ=60)
                                ACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGA                                                              <  SRR4302545.9312040/94‑1 (MQ=60)
                                ACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGA                                                              >  SRR4302545.7942136/1‑94 (MQ=60)
                                   AAATAGCGTCCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGG                                                           <  SRR4302545.10705485/94‑1 (MQ=60)
                                    AATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGC                                                          >  SRR4302545.2443514/1‑94 (MQ=60)
                                    AATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGC                                                          <  SRR4302545.5968451/94‑1 (MQ=60)
                                    AATAGCGTTCCCTGGGCCACGCCATACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGC                                                          <  SRR4302545.5968454/94‑1 (MQ=60)
                                     ATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCG                                                         <  SRR4302545.6200804/94‑1 (MQ=60)
                                     ATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCG                                                         >  SRR4302545.2281507/1‑94 (MQ=60)
                                       AGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGAT                                                       <  SRR4302545.4829619/94‑1 (MQ=60)
                                       AGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGAT                                                       >  SRR4302545.8847380/1‑94 (MQ=60)
                                        GCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATG                                                      <  SRR4302545.9096611/94‑1 (MQ=60)
                                          GTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGT                                                    <  SRR4302545.6907382/94‑1 (MQ=60)
                                          GTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGT                                                    <  SRR4302545.8822924/94‑1 (MQ=60)
                                          GTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGT                                                    >  SRR4302545.10233955/1‑94 (MQ=60)
                                          GTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGCTCGTGAAGGCGATGGT                                                    >  SRR4302545.1232417/1‑94 (MQ=60)
                                           TTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTA                                                   >  SRR4302545.6578985/1‑94 (MQ=60)
                                           TTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTA                                                   >  SRR4302545.10487096/1‑94 (MQ=60)
                                            TCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAG                                                  <  SRR4302545.9798882/94‑1 (MQ=60)
                                            TCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAG                                                  <  SRR4302545.9572947/94‑1 (MQ=60)
                                             CCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGG                                                 <  SRR4302545.1634334/94‑1 (MQ=60)
                                             CCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGG                                                 <  SRR4302545.5415002/94‑1 (MQ=60)
                                             CCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGG                                                 <  SRR4302545.5589311/94‑1 (MQ=60)
                                               CTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCT                                               <  SRR4302545.7396223/94‑1 (MQ=60)
                                                TGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGTCCGAGTTCGTGAAGGCGATGGTAGGCTT                                              >  SRR4302545.46607/1‑94 (MQ=60)
                                                TGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTT                                              >  SRR4302545.1058493/1‑94 (MQ=60)
                                                TGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTT                                              >  SRR4302545.2718391/1‑94 (MQ=60)
                                                TGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTT                                              >  SRR4302545.9626459/1‑94 (MQ=60)
                                                TGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTT                                              >  SRR4302545.1070263/1‑94 (MQ=60)
                                                TGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTT                                              <  SRR4302545.5719055/94‑1 (MQ=60)
                                                TGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTT                                              >  SRR4302545.6411327/1‑94 (MQ=60)
                                                 GGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTC                                             >  SRR4302545.4870259/1‑94 (MQ=60)
                                                  GGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCG                                            >  SRR4302545.1958434/1‑94 (MQ=60)
                                                  GGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCG                                            <  SRR4302545.4777553/94‑1 (MQ=60)
                                                   GCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGA                                           <  SRR4302545.8302607/94‑1 (MQ=60)
                                                    CCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAC                                          >  SRR4302545.4135640/1‑94 (MQ=60)
                                                    CCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGAC                                          >  SRR4302545.7707592/1‑94 (MQ=60)
                                                     CACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACC                                         >  SRR4302545.774023/1‑94 (MQ=60)
                                                     CACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACC                                         <  SRR4302545.9162565/94‑1 (MQ=60)
                                                     CACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACC                                         >  SRR4302545.5381/1‑94 (MQ=60)
                                                      ACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCA                                        >  SRR4302545.9857202/1‑94 (MQ=60)
                                                      ACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCA                                        >  SRR4302545.1273087/1‑94 (MQ=60)
                                                       CGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCAC                                       <  SRR4302545.3951995/94‑1 (MQ=60)
                                                        GCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACG                                      >  SRR4302545.1902152/1‑94 (MQ=60)
                                                          CGTACATCCCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCC                                    <  SRR4302545.3508330/94‑1 (MQ=60)
                                                          CGTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCC                                    >  SRR4302545.2765234/1‑94 (MQ=60)
                                                           GTACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCA                                   >  SRR4302545.1166986/1‑94 (MQ=60)
                                                             ACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTCGGCTTCGACCACGCCACG                                 >  SRR4302545.4996057/1‑94 (MQ=60)
                                                             ACATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACG                                 >  SRR4302545.2088902/1‑94 (MQ=60)
                                                              CATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGC                                >  SRR4302545.4109238/1‑94 (MQ=60)
                                                              CATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGC                                <  SRR4302545.7601035/94‑1 (MQ=60)
                                                               ATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCCCGCCACGCG                               <  SRR4302545.3713490/94‑1 (MQ=60)
                                                               ATACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCG                               >  SRR4302545.10899481/1‑94 (MQ=60)
                                                                 ACCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTG                             >  SRR4302545.10277311/1‑94 (MQ=60)
                                                                  CCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGA                            <  SRR4302545.762991/94‑1 (MQ=60)
                                                                  CCGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGA                            >  SRR4302545.4013704/1‑94 (MQ=60)
                                                                   CGCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGAT                           <  SRR4302545.8154893/94‑1 (MQ=60)
                                                                    GCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATC                          >  SRR4302545.10126558/1‑94 (MQ=60)
                                                                    GCCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATC                          >  SRR4302545.2490427/1‑94 (MQ=60)
                                                                     CCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTTGACCACGCCACGCATGATCC                         >  SRR4302545.5695614/1‑94 (MQ=60)
                                                                     CCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCC                         >  SRR4302545.8236397/1‑94 (MQ=60)
                                                                     CCGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCC                         >  SRR4302545.8236398/1‑94 (MQ=60)
                                                                      CGACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCA                        <  SRR4302545.4652574/94‑1 (MQ=60)
                                                                       GACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAG                       >  SRR4302545.5671058/1‑94 (MQ=60)
                                                                       GACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAG                       >  SRR4302545.1962066/1‑94 (MQ=60)
                                                                       GACAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAG                       >  SRR4302545.8604795/1‑94 (MQ=60)
                                                                         CAAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGT                     <  SRR4302545.9279932/94‑1 (MQ=60)
                                                                         CAAGCACATCTTCACGCCAGGCTTCAGTGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGATAGGCTTCGACCACGCCACGCGTGATCCAGGT                     <  SRR4302545.8302537/94‑1 (MQ=60)
                                                                          AAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTT                    <  SRR4302545.4935893/94‑1 (MQ=60)
                                                                          AAGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTT                    >  SRR4302545.4548149/1‑94 (MQ=60)
                                                                           AGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGTTAGGCTTCGACCACGCCACGCGTGATCCAGGTTC                   <  SRR4302545.6925764/94‑1 (MQ=60)
                                                                           AGCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTC                   <  SRR4302545.6926033/94‑1 (MQ=60)
                                                                            GCTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCC                  >  SRR4302545.7335695/1‑94 (MQ=60)
                                                                             CTCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCT                 <  SRR4302545.535129/94‑1 (MQ=60)
                                                                              TCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGCTCCAGGTTCCTT                >  SRR4302545.959843/1‑94 (MQ=60)
                                                                              TCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTT                <  SRR4302545.4417401/94‑1 (MQ=60)
                                                                              TCATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGGTCCTT                >  SRR4302545.9617774/1‑94 (MQ=60)
                                                                               CATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTC               <  SRR4302545.1522833/94‑1 (MQ=60)
                                                                                ATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCCCGCCACGCGTGATCCAGGTTCCTTCT              >  SRR4302545.5115432/1‑94 (MQ=60)
                                                                                ATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCT              >  SRR4302545.5115433/1‑94 (MQ=60)
                                                                                ATCTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCT              >  SRR4302545.4688071/1‑94 (MQ=60)
                                                                                  CTTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTC            >  SRR4302545.6137877/1‑94 (MQ=60)
                                                                                   TTCACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCG           <  SRR4302545.2512896/94‑1 (MQ=60)
                                                                                     CACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCG         <  SRR4302545.8134721/94‑1 (MQ=60)
                                                                                     CACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCG         >  SRR4302545.9039757/1‑94 (MQ=60)
                                                                                     CACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCG         >  SRR4302545.2068585/1‑94 (MQ=60)
                                                                                      ACGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGA        >  SRR4302545.2929296/1‑94 (MQ=60)
                                                                                       CGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGAT       >  SRR4302545.10166215/1‑94 (MQ=60)
                                                                                       CGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGAT       >  SRR4302545.10166217/1‑94 (MQ=60)
                                                                                       CGCCAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGAT       <  SRR4302545.7230049/94‑1 (MQ=60)
                                                                                          CAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGC    <  SRR4302545.3159823/94‑1 (MQ=60)
                                                                                          CAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGC    >  SRR4302545.9269441/1‑94 (MQ=60)
                                                                                          CAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGC    >  SRR4302545.1280839/1‑94 (MQ=60)
                                                                                          CAGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGC    >  SRR4302545.7621976/1‑94 (MQ=60)
                                                                                           AGGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCT   <  SRR4302545.10186959/94‑1 (MQ=60)
                                                                                            GGCTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTG  <  SRR4302545.8761210/94‑1 (MQ=60)
                                                                                             |                                                                                            
GCATTTTCCATCCGGCTGAACATGGACTCGCCACAAAATAGCGTTCCCTGGGCCACGCCGTACATACCGCCGACAAGCTCATCTTCACGCCAGACTTCAATGGAGTGGGCATGGCCGAGTTCGTGAAGGCGATGGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTG  >  NC_000913/926162‑926347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑