Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,961,301 G→A P592S (CCT→TCT)  flhA ← FlhA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,961,3010GA95.8% 748.9 / 10.6 216P592S (CCT→TCT) flhAFlhA
Reads supporting (aligned to +/- strand):  ref base G (3/6);  new base A (117/90);  total (120/96)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 223 total aligned reads displayed.

GCTCCAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCG  >  NC_000913/1961211‑1961390
                                                                                          |                                                                                         
 cTCCAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGaaaa                                                                                        <  1:2218550/93‑1 (MQ=255)
  tCCAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAAc                                                                                       >  2:5603376/1‑93 (MQ=255)
   ccAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAAcc                                                                                      <  1:2619111/93‑1 (MQ=255)
   ccAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAAcc                                                                                      >  2:4967719/1‑93 (MQ=255)
   ccAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAAcc                                                                                      >  1:2156331/1‑93 (MQ=255)
    cAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGGAAACCa                                                                                     <  1:6077477/93‑1 (MQ=255)
     aGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCAc                                                                                    >  2:4135715/1‑93 (MQ=255)
      gTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCAct                                                                                   <  1:42530/93‑1 (MQ=255)
      gTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCAct                                                                                   <  2:5492958/93‑1 (MQ=255)
      gTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCAct                                                                                   <  2:3937657/93‑1 (MQ=255)
       tcctccCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCActg                                                                                  >  1:2359994/1‑93 (MQ=255)
         ctccCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCActgct                                                                                >  2:4480906/1‑93 (MQ=255)
          tccCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCActgctg                                                                               >  1:4929304/1‑93 (MQ=255)
          tccCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCActgctg                                                                               >  2:1831786/1‑93 (MQ=255)
           cccccGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTgg                                                                              <  1:4814083/93‑1 (MQ=255)
             cccGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGt                                                                            <  1:152982/93‑1 (MQ=255)
              ccGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTa                                                                           >  1:4623596/1‑93 (MQ=255)
               cGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTaa                                                                          <  2:1064679/93‑1 (MQ=255)
               cGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTaa                                                                          <  2:1651124/93‑1 (MQ=255)
               cGCCCTGCAGCGCCTGTAGTAACAAACGTTCCACCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGACAGAAAAGCACTGCTGGGTaa                                                                          >  2:2209340/1‑93 (MQ=255)
                gCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAAt                                                                         >  1:1269269/1‑93 (MQ=255)
                gCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAAt                                                                         >  2:3666791/1‑93 (MQ=255)
                gCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAAt                                                                         <  2:2932206/93‑1 (MQ=255)
                 cccTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATACCATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATc                                                                        <  2:2761516/93‑1 (MQ=255)
                   cTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGc                                                                      <  1:134575/93‑1 (MQ=255)
                   cTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGc                                                                      >  2:3864633/1‑93 (MQ=255)
                          gcCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTccc                                                               <  1:2785715/93‑1 (MQ=255)
                           ccTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCa                                                              >  1:190995/1‑93 (MQ=255)
                           ccTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCa                                                              >  1:3195034/1‑93 (MQ=255)
                            cTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCaa                                                             <  1:1493366/93‑1 (MQ=255)
                            cTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCaa                                                             >  2:5416073/1‑93 (MQ=255)
                            cTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCaa                                                             <  1:799002/93‑1 (MQ=255)
                               tagtaACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCAGTCCCAACGc                                                          >  2:372197/1‑93 (MQ=255)
                               tagtaACAAACGTTCCAGCGGCGTATCGAGGCCCATAACCTGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAATCGCCCGTCCCCAccc                                                          >  2:1564944/1‑91 (MQ=255)
                                 gtaACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCa                                                        >  1:531263/1‑93 (MQ=255)
                                   aaCAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCAcg                                                      >  2:2109630/1‑93 (MQ=255)
                                   aaCAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCAcg                                                      >  1:1742435/1‑93 (MQ=255)
                                    aCAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCAcgc                                                     <  2:1554992/93‑1 (MQ=255)
                                       aaCGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCa                                                  <  2:2318829/93‑1 (MQ=255)
                                       aaCGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCa                                                  <  2:5460110/93‑1 (MQ=255)
                                        aCGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCac                                                 <  2:5985961/93‑1 (MQ=255)
                                           ttCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCacgac                                              <  2:6382739/93‑1 (MQ=255)
                                           ttCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCacgac                                              >  1:3919657/1‑93 (MQ=255)
                                              cAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGAcggc                                           >  1:1809215/1‑93 (MQ=255)
                                              cAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGAcggc                                           >  1:3040510/1‑93 (MQ=255)
                                              cAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGAcggc                                           >  2:2564305/1‑93 (MQ=255)
                                               aGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGAcggcg                                          >  1:4967445/1‑93 (MQ=255)
                                               aGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGAcggcg                                          >  2:5855938/1‑93 (MQ=255)
                                                gCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGCAATCGCCCGTCCCAACGCCCCGCGCACGCcggcgg                                         >  2:5865900/1‑93 (MQ=255)
                                                 cGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGt                                        <  1:3275843/93‑1 (MQ=255)
                                                  ggTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGtt                                       >  1:1355511/1‑93 (MQ=255)
                                                       aTCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTc                                  <  2:1393886/93‑1 (MQ=255)
                                                       aTCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTc                                  >  1:1166055/1‑93 (MQ=255)
                                                       aTCGAGGCCAAAAACATGGACTTCATCTTTGCCAGAAACCCACTGCTGGGTAACCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTc                                  <  2:1373519/93‑1 (MQ=255)
                                                        tCGAGGCCAATAACATGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGCTAATTCa                                 >  2:5113465/1‑93 (MQ=255)
                                                        tCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCa                                 <  2:5557015/93‑1 (MQ=255)
                                                        tCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCa                                 <  1:3745937/93‑1 (MQ=255)
                                                        tCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCa                                 <  1:1580664/93‑1 (MQ=255)
                                                         cGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCAt                                <  1:904944/93‑1 (MQ=255)
                                                           aGGCCAATAACATGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCAtgt                              <  1:6041722/93‑1 (MQ=255)
                                                           aGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCAtgt                              <  2:3368309/93‑1 (MQ=255)
                                                            ggCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCAtgtg                             <  2:4638241/93‑1 (MQ=255)
                                                             gCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTgg                            <  2:323044/93‑1 (MQ=255)
                                                              ccAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGa                           <  1:5662954/93‑1 (MQ=255)
                                                               cAATAACATGGACTTCATCTTTTCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGAt                          <  1:615110/93‑1 (MQ=255)
                                                               cAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGAt                          <  2:6471808/93‑1 (MQ=255)
                                                               cAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGAt                          >  1:4420205/1‑93 (MQ=255)
                                                                 aTAACATGTACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCg                        <  2:4182551/93‑1 (MQ=255)
                                                                 aTAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCg                        >  2:4306183/1‑93 (MQ=255)
                                                                   aaCATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCt                      >  1:5846761/1‑93 (MQ=255)
                                                                    aCATGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCtt                     <  1:1401692/93‑1 (MQ=255)
                                                                    aCATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGTGGTTAATTCATGTGGATCGCtt                     <  2:126040/93‑1 (MQ=255)
                                                                     cATGGACTGCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCttt                    >  2:6304565/1‑93 (MQ=255)
                                                                      aTGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTg                   <  1:5133359/93‑1 (MQ=255)
                                                                       tGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTgg                  >  1:3569655/1‑93 (MQ=255)
                                                                       tGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTgg                  >  1:5289316/1‑93 (MQ=255)
                                                                        ggACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGa                 >  2:1328561/1‑93 (MQ=255)
                                                                        ggACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGa                 <  2:1115827/93‑1 (MQ=255)
                                                                         gACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGAt                >  2:2874192/1‑93 (MQ=255)
                                                                         gACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGAt                <  1:624216/93‑1 (MQ=255)
                                                                         gACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGAt                <  1:1132411/93‑1 (MQ=255)
                                                                          aCTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATg               <  2:3381564/93‑1 (MQ=255)
                                                                          aCTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATg               <  1:1864016/93‑1 (MQ=255)
                                                                          aCTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATg               >  1:2303390/1‑93 (MQ=255)
                                                                           cTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATgg              >  2:1968860/1‑93 (MQ=255)
                                                                           cTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATgg              <  1:317361/93‑1 (MQ=255)
                                                                           cTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATgg              >  1:414653/1‑93 (MQ=255)
                                                                           cTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATgg              >  1:6329890/1‑93 (MQ=255)
                                                                            ttCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATggg             <  1:4474772/93‑1 (MQ=255)
                                                                            ttCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATggc             >  1:5335617/1‑92 (MQ=255)
                                                                             tCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGgc            <  1:5061202/93‑1 (MQ=255)
                                                                              cATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGgcg           >  2:2796302/1‑93 (MQ=255)
                                                                                tCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCa         >  1:5760383/1‑93 (MQ=255)
                                                                                tCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCa         >  2:719997/1‑93 (MQ=255)
                                                                                  tttGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATg       <  1:2438389/93‑1 (MQ=255)
                                                                                  tttGCAAGAAAACCACTGCTGGTAAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATg       <  2:5704286/93‑1 (MQ=255)
                                                                                      ccAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTcc   <  2:811108/93‑1 (MQ=255)
                                                                                      ccAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTcc   <  1:5879063/93‑1 (MQ=255)
                                                                                      ccAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTcc   >  2:3440993/1‑93 (MQ=255)
                                                                                       cAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCg  >  2:5349121/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCTCCAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCG  >  NC_000913/1961211‑1961390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGGCTCCAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCG  >  NC_000913/1961208‑1961390
                                                                                             |                                                                                         
CTGGCTCCAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGA                                                                                           >  SRR4302537.6030357/1‑94 (MQ=60)
    CTCCAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAAC                                                                                       <  SRR4302537.2234187/94‑1 (MQ=60)
     TCCAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACC                                                                                      >  SRR4302537.2171528/1‑94 (MQ=60)
     TCCAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACC                                                                                      <  SRR4302537.1482342/94‑1 (MQ=60)
      CCAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCA                                                                                     <  SRR4302537.4609934/94‑1 (MQ=60)
      CCAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCA                                                                                     <  SRR4302537.2637419/94‑1 (MQ=60)
       CAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGGAAACCAC                                                                                    <  SRR4302537.6119237/94‑1 (MQ=60)
       CAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCAC                                                                                    <  SRR4302537.6380403/94‑1 (MQ=60)
       CAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCAC                                                                                    >  SRR4302537.5142313/1‑94 (MQ=60)
        AGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACT                                                                                   <  SRR4302537.1711316/94‑1 (MQ=60)
         GTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTG                                                                                  <  SRR4302537.42849/94‑1 (MQ=60)
         GTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTG                                                                                  >  SRR4302537.2376637/1‑94 (MQ=60)
         GTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGGTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTG                                                                                  >  SRR4302537.1603303/1‑94 (MQ=60)
           CCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCT                                                                                <  SRR4302537.905553/94‑1 (MQ=60)
            CTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTG                                                                               >  SRR4302537.4963282/1‑94 (MQ=60)
            CTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTG                                                                               >  SRR4302537.1758520/1‑94 (MQ=60)
             TCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGG                                                                              >  SRR4302537.5729232/1‑94 (MQ=60)
             CCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGG                                                                              <  SRR4302537.618896/94‑1 (MQ=60)
              CCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGG                                                                             <  SRR4302537.4847313/94‑1 (MQ=60)
                CCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTA                                                                           <  SRR4302537.154074/94‑1 (MQ=60)
                CCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTA                                                                           >  SRR4302537.4655597/1‑94 (MQ=60)
                  CGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAAT                                                                         >  SRR4302537.3754745/1‑94 (MQ=60)
                  CGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAAT                                                                         >  SRR4302537.4600265/1‑94 (MQ=60)
                  CGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAAT                                                                         >  SRR4302537.1278098/1‑94 (MQ=60)
                   GCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATC                                                                        >  SRR4302537.1576331/1‑94 (MQ=60)
                   GCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATC                                                                        <  SRR4302537.2401459/94‑1 (MQ=60)
                    CCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCG                                                                       >  SRR4302537.1525480/1‑94 (MQ=60)
                     CCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAAGCGC                                                                      >  SRR4302537.5981650/1‑94 (MQ=60)
                      CTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCC                                                                     <  SRR4302537.135528/94‑1 (MQ=60)
                            CGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCC                                                               >  SRR4302537.6265198/1‑94 (MQ=60)
                             GCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCA                                                              <  SRR4302537.2805193/94‑1 (MQ=60)
                             GCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCA                                                              >  SRR4302537.192352/1‑94 (MQ=60)
                             GCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCA                                                              >  SRR4302537.3217499/1‑94 (MQ=60)
                               CTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAAC                                                            <  SRR4302537.804488/94‑1 (MQ=60)
                               CTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAAC                                                            <  SRR4302537.1503768/94‑1 (MQ=60)
                                 GTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCTTCCCAACGC                                                          >  SRR4302537.1370590/1‑94 (MQ=60)
                                   AGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCA                                                        >  SRR4302537.534910/1‑94 (MQ=60)
                                   AGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCA                                                        >  SRR4302537.6080632/1‑94 (MQ=60)
                                    GTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCAC                                                       <  SRR4302537.1962019/94‑1 (MQ=60)
                                    GTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCAC                                                       >  SRR4302537.5426773/1‑94 (MQ=60)
                                     TAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACG                                                      >  SRR4302537.1754583/1‑94 (MQ=60)
                                            CGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGC                                               >  SRR4302537.3980556/1‑94 (MQ=60)
                                            CGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGA                                               >  SRR4302537.5367647/1‑94 (MQ=60)
                                             GTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGAC                                              >  SRR4302537.3947053/1‑94 (MQ=60)
                                             GTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGAC                                              <  SRR4302537.2490451/94‑1 (MQ=60)
                                                CCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGC                                           >  SRR4302537.3061698/1‑94 (MQ=60)
                                                CCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGC                                           >  SRR4302537.1821834/1‑94 (MQ=60)
                                                 CAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCG                                          >  SRR4302537.218/1‑94 (MQ=60)
                                                 CAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCG                                          >  SRR4302537.5001682/1‑94 (MQ=60)
                                                    CGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTT                                       >  SRR4302537.3201831/1‑94 (MQ=60)
                                                    CGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTT                                       <  SRR4302537.5832794/94‑1 (MQ=60)
                                                    CGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTT                                       >  SRR4302537.3848470/1‑94 (MQ=60)
                                                    CGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTT                                       >  SRR4302537.1364957/1‑94 (MQ=60)
                                                    CGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTT                                       <  SRR4302537.3298960/94‑1 (MQ=60)
                                                      GTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAA                                     >  SRR4302537.1301778/1‑94 (MQ=60)
                                                       TGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAAT                                    >  SRR4302537.3499605/1‑94 (MQ=60)
                                                       TGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAAT                                    <  SRR4302537.5075462/94‑1 (MQ=60)
                                                       TGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCACAACGCCACGCGCACGACGGCGGTTAAT                                    >  SRR4302537.591202/1‑94 (MQ=60)
                                                        GTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATT                                   <  SRR4302537.5637694/94‑1 (MQ=60)
                                                         TATCGAGGCCAATAACATGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTC                                  <  SRR4302537.4974068/94‑1 (MQ=60)
                                                         TATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTC                                  >  SRR4302537.1174162/1‑94 (MQ=60)
                                                          ATCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCA                                 >  SRR4302537.2601353/1‑94 (MQ=60)
                                                           TCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCAT                                <  SRR4302537.3772244/94‑1 (MQ=60)
                                                           TCGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCAT                                <  SRR4302537.1591711/94‑1 (MQ=60)
                                                            CGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATG                               <  SRR4302537.911189/94‑1 (MQ=60)
                                                            CGAGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACCGCGGGTAATTTATG                               >  SRR4302537.1841194/1‑94 (MQ=60)
                                                              AGGCCAATAACATGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTG                             <  SRR4302537.6083225/94‑1 (MQ=60)
                                                              AGGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTG                             >  SRR4302537.628773/1‑94 (MQ=60)
                                                               GGCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGG                            <  SRR4302537.6105916/94‑1 (MQ=60)
                                                                GCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGA                           <  SRR4302537.1152492/94‑1 (MQ=60)
                                                                GCCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGA                           >  SRR4302537.5173009/1‑94 (MQ=60)
                                                                 CCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGAT                          >  SRR4302537.296546/1‑94 (MQ=60)
                                                                 CCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGAT                          <  SRR4302537.5701701/94‑1 (MQ=60)
                                                                 CCAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGAT                          >  SRR4302537.4450723/1‑94 (MQ=60)
                                                                  CAATAACATGGACTTCATCTTTTCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATC                         <  SRR4302537.619300/94‑1 (MQ=60)
                                                                  CAATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATC                         >  SRR4302537.1370406/1‑94 (MQ=60)
                                                                   AATAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGCTCG                        >  SRR4302537.4922690/1‑94 (MQ=60)
                                                                     TAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCT                      >  SRR4302537.5886851/1‑94 (MQ=60)
                                                                     TAACATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCCCACGACGGCGGTTAATTCATGTGGATCGCT                      >  SRR4302537.1910910/1‑94 (MQ=60)
                                                                       ACATGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTT                    <  SRR4302537.1411459/94‑1 (MQ=60)
                                                                        CATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTG                   >  SRR4302537.5339377/1‑94 (MQ=60)
                                                                        CATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTG                   >  SRR4302537.2709292/1‑94 (MQ=60)
                                                                         ATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGG                  <  SRR4302537.5168730/94‑1 (MQ=60)
                                                                         ATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGG                  >  SRR4302537.5325671/1‑94 (MQ=60)
                                                                         ATGGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGG                  >  SRR4302537.3594826/1‑94 (MQ=60)
                                                                           GGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGAT                <  SRR4302537.419977/94‑1 (MQ=60)
                                                                           GGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGAT                <  SRR4302537.3091046/94‑1 (MQ=60)
                                                                           GGACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGAT                >  SRR4302537.2212261/1‑94 (MQ=60)
                                                                            GACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATG               >  SRR4302537.2319648/1‑94 (MQ=60)
                                                                            GACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATG               <  SRR4302537.1140273/94‑1 (MQ=60)
                                                                            GACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATG               <  SRR4302537.628475/94‑1 (MQ=60)
                                                                             ACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGG              <  SRR4302537.2262525/94‑1 (MQ=60)
                                                                             ACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGG              >  SRR4302537.6373173/1‑94 (MQ=60)
                                                                             ACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGG              <  SRR4302537.1876995/94‑1 (MQ=60)
                                                                             ACTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGG              >  SRR4302537.417526/1‑94 (MQ=60)
                                                                              CTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGG             >  SRR4302537.1223000/1‑94 (MQ=60)
                                                                              CTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGG             <  SRR4302537.319561/94‑1 (MQ=60)
                                                                              CTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGG             <  SRR4302537.4752844/94‑1 (MQ=60)
                                                                              CTTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGC             >  SRR4302537.5372203/1‑94 (MQ=60)
                                                                               TTCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGC            <  SRR4302537.4505729/94‑1 (MQ=60)
                                                                               TTCATCTTTGCCAGAAAACCACTGCTGGGTAATAGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCACGTGGATCGCTTTGGATGGGC            >  SRR4302537.3218778/1‑94 (MQ=60)
                                                                                TCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTATTTCATGTGGATCGCTTTGGATGGGCG           >  SRR4302537.4393158/1‑94 (MQ=60)
                                                                                TCATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCG           <  SRR4302537.5096045/94‑1 (MQ=60)
                                                                                  ATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCA         >  SRR4302537.2335924/1‑94 (MQ=60)
                                                                                  ATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCA         >  SRR4302537.5799840/1‑94 (MQ=60)
                                                                                  ATCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTAATGTGGATCGCTTTGGATGGGCGCA         <  SRR4302537.5017816/94‑1 (MQ=60)
                                                                                   TCTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCAT        <  SRR4302537.3203223/94‑1 (MQ=60)
                                                                                    TTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATG       <  SRR4302537.4112314/94‑1 (MQ=60)
                                                                                    CTTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATG       >  SRR4302537.6301475/1‑94 (MQ=60)
                                                                                     TTTGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGT      <  SRR4302537.2455566/94‑1 (MQ=60)
                                                                                       TGCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTC    >  SRR4302537.4464302/1‑94 (MQ=60)
                                                                                        GCCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCC   <  SRR4302537.1363466/94‑1 (MQ=60)
                                                                                         CCAGAAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCG  <  SRR4302537.5919396/94‑1 (MQ=60)
                                                                                             |                                                                                         
CTGGCTCCAGTCCTCCCCCGCCCTGCAGCGCCTGTAGTAACAAACGTTCCAGCGGTGTATCGAGGCCAATAACATGGACTTCATCTTTGCCAGGAAACCACTGCTGGGTAATCGCCCGTCCCAACGCCACGCGCACGACGGCGGTTAATTCATGTGGATCGCTTTGGATGGGCGCATGTTCCG  >  NC_000913/1961208‑1961390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑