Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,963,151 A→G A355A (GCT→GCC)  flhB ← FlhB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,963,1510AG99.4% 607.5 / ‑4.5 179A355A (GCT→GCC) flhBFlhB
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (99/79);  total (100/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-01

Only 100 of 199 total aligned reads displayed.

GCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATC  >  NC_000913/1963061‑1963238
                                                                                          |                                                                                       
gCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccggc                                                                                       >  2:1422977/1‑93 (MQ=255)
gCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccggc                                                                                       >  2:6018128/1‑93 (MQ=255)
 ccAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccggcc                                                                                      >  2:4858198/1‑93 (MQ=255)
  cAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccggcca                                                                                     >  2:1038518/1‑93 (MQ=255)
    gATTACTCATGGGTCGGTTTCTCGTTAATAAAAGCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGa                                                                                   <  1:2526730/93‑1 (MQ=255)
    gATTACTCATGGGTCGGTTTATCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGa                                                                                   <  2:4253239/93‑1 (MQ=255)
     aTTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGAc                                                                                  <  2:3756338/93‑1 (MQ=255)
     aTTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGAc                                                                                  <  2:1105470/93‑1 (MQ=255)
      ttACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACg                                                                                 <  1:4952310/93‑1 (MQ=255)
        aCTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGcc                                                                               >  1:2927414/1‑93 (MQ=255)
        aCTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGcc                                                                               >  2:3830555/1‑93 (MQ=255)
         cTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGccg                                                                              >  1:2217781/1‑92 (MQ=255)
         cTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCa                                                                              >  2:5896296/1‑93 (MQ=255)
          tCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAg                                                                             <  1:1732385/93‑1 (MQ=255)
          tCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAg                                                                             <  1:2956951/93‑1 (MQ=255)
          tCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAg                                                                             >  2:2311077/1‑93 (MQ=255)
           cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGc                                                                            >  2:813927/1‑93 (MQ=255)
            aTGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCg                                                                           <  1:1817121/93‑1 (MQ=255)
            aTGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCg                                                                           <  2:6302254/93‑1 (MQ=255)
              gggTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGtt                                                                         >  1:4875088/1‑93 (MQ=255)
                gTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTc                                                                       >  1:6105168/1‑93 (MQ=255)
                  cGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCat                                                                     >  1:3095520/1‑92 (MQ=255)
                  cGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAg                                                                     >  1:907970/1‑93 (MQ=255)
                   ggTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGt                                                                    <  2:2933689/93‑1 (MQ=255)
                   ggTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGt                                                                    <  2:3218234/93‑1 (MQ=255)
                    gTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGtt                                                                   >  1:1908826/1‑93 (MQ=255)
                      ttCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGc                                                                 <  2:2474523/93‑1 (MQ=255)
                      ttCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGACAGCGATTCAGTTGc                                                                 >  2:3946633/1‑93 (MQ=255)
                       tctcGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGcc                                                                >  1:5246280/1‑93 (MQ=255)
                        ctcGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCa                                                               >  1:1017834/1‑93 (MQ=255)
                        ctcGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCa                                                               >  1:1670682/1‑93 (MQ=255)
                        ctcGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCa                                                               <  1:6226333/93‑1 (MQ=255)
                        ctcGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGGAGGTTGTGCAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCa                                                               >  2:5307363/1‑93 (MQ=255)
                         tcGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAg                                                              >  2:701399/1‑93 (MQ=255)
                          cGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGa                                                             <  1:5729047/93‑1 (MQ=255)
                           gTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGAc                                                            >  2:4324448/1‑93 (MQ=255)
                            ttAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGAcc                                                           >  2:5938452/1‑93 (MQ=255)
                             taataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGAccc                                                          >  2:1960362/1‑93 (MQ=255)
                              aataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCa                                                         <  2:4435554/93‑1 (MQ=255)
                              aataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCa                                                         <  2:5476461/93‑1 (MQ=255)
                               ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAg                                                        <  1:5460640/93‑1 (MQ=255)
                               ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAg                                                        <  1:3562409/93‑1 (MQ=255)
                                taaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAgg                                                       <  2:2193591/93‑1 (MQ=255)
                                  aaaTCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGcc                                                     >  1:533566/1‑93 (MQ=255)
                                   aaTCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCa                                                    <  2:4669730/93‑1 (MQ=255)
                                    aTCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAg                                                   >  2:2593513/1‑93 (MQ=255)
                                      ccAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                                                 >  2:4928992/1‑93 (MQ=255)
                                        aGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACt                                               >  2:326280/1‑93 (MQ=255)
                                          ggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTc                                             <  2:1000332/93‑1 (MQ=255)
                                          ggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTc                                             <  1:3639110/93‑1 (MQ=255)
                                          ggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTc                                             <  1:849989/93‑1 (MQ=255)
                                           gCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTcc                                            <  1:3925184/93‑1 (MQ=255)
                                           gCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTcc                                            <  2:4052128/93‑1 (MQ=255)
                                           gCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTcc                                            <  1:271671/93‑1 (MQ=255)
                                           gCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTcc                                            >  1:1184473/1‑93 (MQ=255)
                                           gCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGACAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTcc                                            <  1:2109004/93‑1 (MQ=255)
                                            cTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCg                                           <  2:2011112/93‑1 (MQ=255)
                                             ttCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGc                                          >  1:1633024/1‑93 (MQ=255)
                                             ttCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGc                                          >  1:1530884/1‑93 (MQ=255)
                                              tCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGcc                                         <  1:6475336/93‑1 (MQ=255)
                                              tCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGcc                                         >  1:2357028/1‑93 (MQ=255)
                                               ccGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCa                                        >  1:3411965/1‑93 (MQ=255)
                                               ccGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCa                                        >  2:2685714/1‑93 (MQ=255)
                                                cGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAc                                       >  1:1080217/1‑93 (MQ=255)
                                                cGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAc                                       <  1:5201582/93‑1 (MQ=255)
                                                 ggCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAcc                                      >  1:4111703/1‑93 (MQ=255)
                                                 ggCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAcc                                      >  1:1123984/1‑93 (MQ=255)
                                                  gCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACcg                                     >  1:3819667/1‑93 (MQ=255)
                                                   cACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACcgc                                    <  1:2256791/93‑1 (MQ=255)
                                                     ccGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCgcgg                                  <  1:5581511/93‑1 (MQ=255)
                                                     ccGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCgcgg                                  <  1:415526/93‑1 (MQ=255)
                                                      cGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCgcggc                                 >  2:3905365/1‑93 (MQ=255)
                                                       ggAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCgcggcg                                >  1:470710/1‑93 (MQ=255)
                                                        gAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTTCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGt                               >  2:6133291/1‑93 (MQ=255)
                                                        gAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGt                               <  1:1950426/93‑1 (MQ=255)
                                                        gAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGt                               >  1:3472632/1‑93 (MQ=255)
                                                         aaGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTa                              >  2:3484418/1‑93 (MQ=255)
                                                           gATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACa                            >  1:6146036/1‑93 (MQ=255)
                                                             tGAGTAGGTTGTACAGGGCGCTGTCCACCGACCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGt                          <  2:5638089/93‑1 (MQ=255)
                                                              gAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGtt                         <  2:5828995/93‑1 (MQ=255)
                                                               aGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTg                        <  2:3733639/93‑1 (MQ=255)
                                                                gTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGa                       <  1:4988578/93‑1 (MQ=255)
                                                                   ggTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGAccc                    <  1:562348/93‑1 (MQ=255)
                                                                   ggTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGAccc                    <  2:3014359/93‑1 (MQ=255)
                                                                   ggTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGAccc                    <  1:4928992/93‑1 (MQ=255)
                                                                    gTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCg                   >  2:5692834/1‑93 (MQ=255)
                                                                       gTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGa                <  1:4457718/93‑1 (MQ=255)
                                                                       gTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGa                >  1:1369293/1‑93 (MQ=255)
                                                                         aCAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGAtt              >  1:5927161/1‑93 (MQ=255)
                                                                          cAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGAttt             >  1:2512643/1‑93 (MQ=255)
                                                                            gggCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATttgt           <  2:6413696/93‑1 (MQ=255)
                                                                            gggCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATttgt           >  2:3505276/1‑93 (MQ=255)
                                                                             ggCGCTGCCCACCGGCCAGCCGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGTACCGGGGCttgtt          >  2:1268786/1‑93 (MQ=255)
                                                                               cgcTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGa        >  1:3206227/1‑93 (MQ=255)
                                                                               cgcTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGa        <  2:2371334/93‑1 (MQ=255)
                                                                                  tGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCa     >  2:315463/1‑93 (MQ=255)
                                                                                    tCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAAt   >  2:3649757/1‑93 (MQ=255)
                                                                                    tCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAAt   >  1:260314/1‑93 (MQ=255)
                                                                                     ccaccgGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAAtc  <  1:2032245/93‑1 (MQ=255)
                                                                                     ccaccgGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAAtc  <  2:4981376/93‑1 (MQ=255)
                                                                                          |                                                                                       
GCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATC  >  NC_000913/1963061‑1963238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCA  >  NC_000913/1963058‑1963244
                                                                                             |                                                                                             
GCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCG                                                                                               >  SRR4302537.3664751/1‑94 (MQ=60)
 CGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGG                                                                                              >  SRR4302537.4115383/1‑94 (MQ=60)
   GCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCC                                                                                            <  SRR4302537.5522163/94‑1 (MQ=60)
   GCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCC                                                                                            <  SRR4302537.2322250/94‑1 (MQ=60)
     CAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAG                                                                                          >  SRR4302537.1757412/1‑94 (MQ=60)
      AGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGA                                                                                         >  SRR4302537.344611/1‑94 (MQ=60)
       GATTACTCATGGGTCGGTTTCTCGTTAATAAAAGCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGAC                                                                                        <  SRR4302537.2544459/94‑1 (MQ=60)
        ATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACG                                                                                       >  SRR4302537.5385371/1‑94 (MQ=60)
        ATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACC                                                                                       >  SRR4302537.5015339/1‑94 (MQ=60)
         TTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGC                                                                                      <  SRR4302537.4986464/94‑1 (MQ=60)
          TACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCC                                                                                     >  SRR4302537.2947971/1‑94 (MQ=60)
           ACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCG                                                                                    >  SRR4302537.2233417/1‑94 (MQ=60)
             TCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGC                                                                                  <  SRR4302537.2977697/94‑1 (MQ=60)
             TCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGC                                                                                  <  SRR4302537.1744472/94‑1 (MQ=60)
               ATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGT                                                                                <  SRR4302537.1829784/94‑1 (MQ=60)
               ATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGT                                                                                >  SRR4302537.1452118/1‑94 (MQ=60)
                TGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTT                                                                               <  SRR4302537.1977216/94‑1 (MQ=60)
                TGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTT                                                                               >  SRR4302537.2135914/1‑94 (MQ=60)
                TGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTT                                                                               >  SRR4302537.4908737/1‑94 (MQ=60)
                  GGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTC                                                                             >  SRR4302537.6147112/1‑94 (MQ=60)
                  GGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTC                                                                             <  SRR4302537.1167707/94‑1 (MQ=60)
                    TCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAT                                                                           >  SRR4302537.3117120/1‑94 (MQ=60)
                    TCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAG                                                                           >  SRR4302537.1906894/1‑94 (MQ=60)
                    TCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAG                                                                           >  SRR4302537.914243/1‑94 (MQ=60)
                      GGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTT                                                                         >  SRR4302537.1922078/1‑94 (MQ=60)
                      GGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGCTTCAGTT                                                                         >  SRR4302537.879559/1‑94 (MQ=60)
                       GTTTCTCGTTAATAAAATCCAGGGCTTCCGTCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTG                                                                        >  SRR4302537.2061993/1‑94 (MQ=60)
                       GTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTG                                                                        >  SRR4302537.5940737/1‑94 (MQ=60)
                         TTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCC                                                                      >  SRR4302537.838888/1‑94 (MQ=60)
                         TTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCC                                                                      >  SRR4302537.5282379/1‑94 (MQ=60)
                          TCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCA                                                                     >  SRR4302537.1682384/1‑94 (MQ=60)
                          TCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCA                                                                     >  SRR4302537.1024844/1‑94 (MQ=60)
                           CTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAG                                                                    <  SRR4302537.6269179/94‑1 (MQ=60)
                             CGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGAC                                                                  <  SRR4302537.5768263/94‑1 (MQ=60)
                                TAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCA                                                               >  SRR4302537.3180930/1‑94 (MQ=60)
                                TAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCA                                                               >  SRR4302537.1582883/1‑94 (MQ=60)
                                  ATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGG                                                             <  SRR4302537.3587538/94‑1 (MQ=60)
                                  ATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGG                                                             <  SRR4302537.5497889/94‑1 (MQ=60)
                                    AAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCC                                                           >  SRR4302537.537227/1‑94 (MQ=60)
                                     AAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCA                                                          <  SRR4302537.4144760/94‑1 (MQ=60)
                                      AATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAG                                                         >  SRR4302537.2656131/1‑94 (MQ=60)
                                       ATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGC                                                        >  SRR4302537.1687284/1‑94 (MQ=60)
                                       ATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGC                                                        <  SRR4302537.6260001/94‑1 (MQ=60)
                                        TCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCA                                                       >  SRR4302537.5687441/1‑94 (MQ=60)
                                         CCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCAC                                                      <  SRR4302537.2824414/94‑1 (MQ=60)
                                           AGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTT                                                    >  SRR4302537.4975561/1‑94 (MQ=60)
                                            GGGCTTCTGGCACCGCAAGATGAGTAGGTTGTACAGGGTGCTGTCCACCGGACAGGCGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTC                                                   <  SRR4302537.5671874/94‑1 (MQ=60)
                                             GGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCC                                                  >  SRR4302537.3012286/1‑94 (MQ=60)
                                             GGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCC                                                  >  SRR4302537.1192707/1‑94 (MQ=60)
                                             GGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCC                                                  <  SRR4302537.3664738/94‑1 (MQ=60)
                                             GGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCC                                                  >  SRR4302537.1097576/1‑94 (MQ=60)
                                             GGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCC                                                  <  SRR4302537.855817/94‑1 (MQ=60)
                                              GCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCG                                                 <  SRR4302537.273581/94‑1 (MQ=60)
                                              GCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCG                                                 <  SRR4302537.3952606/94‑1 (MQ=60)
                                              GCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGACAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCG                                                 <  SRR4302537.2123872/94‑1 (MQ=60)
                                               CTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGC                                                >  SRR4302537.1644466/1‑94 (MQ=60)
                                               CTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGC                                                >  SRR4302537.1541582/1‑94 (MQ=60)
                                                TTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCC                                               >  SRR4302537.2373651/1‑94 (MQ=60)
                                                 TCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCA                                              >  SRR4302537.3583943/1‑94 (MQ=60)
                                                 TCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCA                                              >  SRR4302537.3436128/1‑94 (MQ=60)
                                                 TCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCA                                              <  SRR4302537.6519298/94‑1 (MQ=60)
                                                  CCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAC                                             >  SRR4302537.1087672/1‑94 (MQ=60)
                                                   CGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACC                                            >  SRR4302537.1131779/1‑94 (MQ=60)
                                                   CGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACC                                            >  SRR4302537.4518949/1‑94 (MQ=60)
                                                   CGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACC                                            <  SRR4302537.3719181/94‑1 (MQ=60)
                                                   CGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACC                                            <  SRR4302537.5237364/94‑1 (MQ=60)
                                                   CGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACC                                            >  SRR4302537.4140423/1‑94 (MQ=60)
                                                    GGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCG                                           >  SRR4302537.3846451/1‑94 (MQ=60)
                                                    GGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCG                                           <  SRR4302537.407887/94‑1 (MQ=60)
                                                      CACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCG                                         <  SRR4302537.2272719/94‑1 (MQ=60)
                                                      CACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCAAGCCCTTCCGCCACCGCG                                         >  SRR4302537.3361793/1‑94 (MQ=60)
                                                       ACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGG                                        >  SRR4302537.1328796/1‑94 (MQ=60)
                                                       ACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGG                                        <  SRR4302537.6057212/94‑1 (MQ=60)
                                                        CCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGC                                       <  SRR4302537.418408/94‑1 (MQ=60)
                                                        CCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGC                                       <  SRR4302537.5619664/94‑1 (MQ=60)
                                                         CGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCG                                      <  SRR4302537.2949037/94‑1 (MQ=60)
                                                         CGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCG                                      >  SRR4302537.473933/1‑94 (MQ=60)
                                                         CGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCG                                      >  SRR4302537.2542255/1‑94 (MQ=60)
                                                          GGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGT                                     >  SRR4302537.3497206/1‑94 (MQ=60)
                                                           GAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTA                                    <  SRR4302537.1963988/94‑1 (MQ=60)
                                                           GAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTA                                    >  SRR4302537.5057076/1‑94 (MQ=60)
                                                             AGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACA                                  >  SRR4302537.6188308/1‑94 (MQ=60)
                                                             AGATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACA                                  >  SRR4302537.5271150/1‑94 (MQ=60)
                                                               ATGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGT                                >  SRR4302537.49163/1‑94 (MQ=60)
                                                                TGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTT                               <  SRR4302537.6179129/94‑1 (MQ=60)
                                                                TGAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTT                               >  SRR4302537.1950328/1‑94 (MQ=60)
                                                                 GAGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTG                              <  SRR4302537.4248200/94‑1 (MQ=60)
                                                                  AGTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGA                             >  SRR4302537.6435796/1‑94 (MQ=60)
                                                                   GTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGAC                            >  SRR4302537.933608/1‑94 (MQ=60)
                                                                   GTAGGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGAC                            <  SRR4302537.5022955/94‑1 (MQ=60)
                                                                      GGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCG                         <  SRR4302537.4962967/94‑1 (MQ=60)
                                                                      GGTTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCG                         <  SRR4302537.566216/94‑1 (MQ=60)
                                                                        TTGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGG                       <  SRR4302537.5333378/94‑1 (MQ=60)
                                                                         TGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGA                      >  SRR4302537.1378832/1‑94 (MQ=60)
                                                                         TGTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGA                      >  SRR4302537.2557796/1‑94 (MQ=60)
                                                                          GTACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGAT                     <  SRR4302537.4488506/94‑1 (MQ=60)
                                                                          GTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGAT                     >  SRR4302537.3919030/1‑94 (MQ=60)
                                                                           TACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATT                    >  SRR4302537.5967839/1‑94 (MQ=60)
                                                                            ACAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTT                   >  SRR4302537.2530309/1‑94 (MQ=60)
                                                                             CAGGGCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTG                  >  SRR4302537.2256233/1‑94 (MQ=60)
                                                                                 GCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGA              <  SRR4302537.5640903/94‑1 (MQ=60)
                                                                                 GCGCTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGA              >  SRR4302537.3228768/1‑94 (MQ=60)
                                                                                    CTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCA           >  SRR4302537.1669975/1‑94 (MQ=60)
                                                                                    CTGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCA           <  SRR4302537.2397511/94‑1 (MQ=60)
                                                                                     TGTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAA          >  SRR4302537.4827172/1‑94 (MQ=60)
                                                                                      GTCCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAAT         >  SRR4302537.262150/1‑94 (MQ=60)
                                                                                        CCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCT       <  SRR4302537.3594201/94‑1 (MQ=60)
                                                                                        CCACCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCT       <  SRR4302537.2046408/94‑1 (MQ=60)
                                                                                           CCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCG    >  SRR4302537.392205/1‑94 (MQ=60)
                                                                                           CCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCG    <  SRR4302537.2659947/94‑1 (MQ=60)
                                                                                           CCGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCG    >  SRR4302537.585699/1‑94 (MQ=60)
                                                                                            CGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGC   <  SRR4302537.2508885/94‑1 (MQ=60)
                                                                                            CGGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGC   <  SRR4302537.330496/94‑1 (MQ=60)
                                                                                             GGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCA  >  SRR4302537.2649844/1‑94 (MQ=60)
                                                                                             GGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCA  >  SRR4302537.3706865/1‑94 (MQ=60)
                                                                                             GGCCAGACGCCAGCGTTTCAGTTGACAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCA  >  SRR4302537.764857/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACAGTTGACCCGGGATTTGTTGACCAATCTCCGCA  >  NC_000913/1963058‑1963244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑