Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,070,275 G→A G239S (GGC→AGC)  b2000 → outer membrane fluffing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,070,2750GA100.0% 670.9 / NA 188G239S (GGC→AGC) b2000outer membrane fluffing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (98/90);  total (98/90)

Only 100 of 202 total aligned reads displayed.

GGGGATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCGGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACT  >  NC_000913/2070185‑2070364
                                                                                          |                                                                                         
ggggATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCggcagc                                                                                         >  1:6475557/1‑93 (MQ=255)
ggggATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCggcagc                                                                                         <  1:5108524/93‑1 (MQ=255)
gaggATGCCGTACCCACATCAAGCAATAAAACCGGTAGCCAGATTGTGAGACCTGACGGAACGGCAAATTCCACTGTGGTTTATGCCggcagc                                                                                         <  2:2811514/91‑1 (MQ=255)
 gggATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGgcagcg                                                                                        <  2:5218070/93‑1 (MQ=255)
  ggctGCCGTACGCACAACCATCAATAAAAACCGTCGCCAGCTTGTGAGAGCTGAAGGAACCGCAAATACCACTGTGGTTTATGCCGGCAGCGa                                                                                       <  2:5794514/90‑1 (MQ=255)
    aTGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGAcc                                                                                     <  1:5018419/93‑1 (MQ=255)
     tGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCa                                                                                    >  2:4805960/1‑93 (MQ=255)
     tGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCa                                                                                    >  2:1700012/1‑93 (MQ=255)
     tGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCa                                                                                    >  2:5892642/1‑93 (MQ=255)
      gCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAg                                                                                   >  2:6406453/1‑93 (MQ=255)
        cGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGAc                                                                                 <  1:182458/93‑1 (MQ=255)
        cGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGAc                                                                                 <  2:4675171/93‑1 (MQ=255)
         gTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACt                                                                                >  1:2329464/1‑93 (MQ=255)
          tACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTg                                                                               <  1:1446894/93‑1 (MQ=255)
          tACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTg                                                                               >  2:3011112/1‑93 (MQ=255)
            cGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTa                                                                             <  1:5299481/93‑1 (MQ=255)
              cacaACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACa                                                                           >  2:1535203/1‑93 (MQ=255)
              cacaACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACa                                                                           <  1:2799892/93‑1 (MQ=255)
              cacaACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACa                                                                           <  2:419349/93‑1 (MQ=255)
               acaACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACAt                                                                          <  2:1778689/93‑1 (MQ=255)
               acaACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACAt                                                                          <  2:5372464/93‑1 (MQ=255)
               acaACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACAt                                                                          >  1:1631084/1‑93 (MQ=255)
                caACCATCAATAAAAACGGTCGCCCGATTGTGAGAGCTGAAGGACCGGCAAATACCACTGTGGTTTATGCAGGCAGCGACCCGAATGTACATg                                                                         >  1:1830971/1‑93 (MQ=255)
                caACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATg                                                                         <  2:1799785/93‑1 (MQ=255)
                caACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATg                                                                         <  1:1730335/93‑1 (MQ=255)
                 aaCCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATgg                                                                        <  2:382125/93‑1 (MQ=255)
                   ccATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTc                                                                      >  2:3702495/1‑93 (MQ=255)
                   ccATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTc                                                                      >  2:5190666/1‑93 (MQ=255)
                   ccATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTc                                                                      <  1:2886484/93‑1 (MQ=255)
                    cATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCa                                                                     <  1:34823/93‑1 (MQ=255)
                    cATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCa                                                                     <  1:3259314/93‑1 (MQ=255)
                       cAATAAAAACTGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGc                                                                  <  2:4266620/93‑1 (MQ=255)
                        aaTAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCa                                                                 >  2:4712890/1‑93 (MQ=255)
                         aTAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCAc                                                                >  1:707729/1‑93 (MQ=255)
                          tAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACt                                                               <  1:4091860/93‑1 (MQ=255)
                           aaaaaCGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTg                                                              <  1:873505/93‑1 (MQ=255)
                           aaaaaCGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTg                                                              <  1:3427378/93‑1 (MQ=255)
                           aaaaaCGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTg                                                              <  2:2016295/93‑1 (MQ=255)
                           aaaaaCGGTCGCAAGATTGTGAGAGCTGAAGGAACGTCAAATGCCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTg                                                              <  2:4148363/93‑1 (MQ=255)
                            aaaaCGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGGACATTGTCACGCACTgg                                                             >  2:4970183/1‑93 (MQ=255)
                             aaaCGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGa                                                            >  2:5407627/1‑93 (MQ=255)
                             aaaCGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGa                                                            >  2:1956253/1‑93 (MQ=255)
                              aaCGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGAt                                                           >  2:4070394/1‑93 (MQ=255)
                                 ggTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATAcc                                                        >  1:4121222/1‑93 (MQ=255)
                                  gTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGAATGTACATGGTCACGCACTGGATACCa                                                       <  2:2395460/93‑1 (MQ=255)
                                     gCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTTGATACCACGc                                                    >  1:6264120/1‑93 (MQ=255)
                                     gCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGc                                                    >  2:5066459/1‑93 (MQ=255)
                                      ccAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCt                                                   <  1:4198677/93‑1 (MQ=255)
                                       cAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTg                                                  >  2:4742992/1‑93 (MQ=255)
                                       cAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTg                                                  >  2:4751088/1‑93 (MQ=255)
                                        aGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGa                                                 >  1:6271303/1‑93 (MQ=255)
                                        aGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTCGATACCACGCTGa                                                 >  1:5580201/1‑93 (MQ=255)
                                         gATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGaa                                                >  2:3766382/1‑93 (MQ=255)
                                         gATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGaa                                                >  2:2534816/1‑93 (MQ=255)
                                         gATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGaa                                                >  1:1042796/1‑93 (MQ=255)
                                           ttGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATg                                              >  1:4019611/1‑93 (MQ=255)
                                            tgtgAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATTCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATgg                                             >  1:1692763/1‑93 (MQ=255)
                                            tgtgAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATgg                                             <  1:5900775/93‑1 (MQ=255)
                                            tgtgAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATgg                                             <  1:5672277/93‑1 (MQ=255)
                                              tgAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATgggg                                           <  2:1889533/93‑1 (MQ=255)
                                              tgAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATgggg                                           <  2:6290654/93‑1 (MQ=255)
                                              tgAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATgggg                                           >  1:3597570/1‑93 (MQ=255)
                                                 gagCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGAt                                        >  1:930243/1‑93 (MQ=255)
                                                  agCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATa                                       <  1:238773/93‑1 (MQ=255)
                                                      gAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAg                                   <  2:2825699/93‑1 (MQ=255)
                                                      gAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAg                                   >  2:107799/1‑93 (MQ=255)
                                                         ggAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTAt                                >  1:4783238/1‑93 (MQ=255)
                                                         ggAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTAt                                <  1:123175/93‑1 (MQ=255)
                                                         ggAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTAt                                >  2:437803/1‑93 (MQ=255)
                                                          gAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTAtg                               >  2:5707468/1‑93 (MQ=255)
                                                            aCGGCAAATACCACTGTGGTTTATGCGGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTAtgtg                             <  2:2778545/93‑1 (MQ=255)
                                                            aCGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTAtgtg                             >  2:3898566/1‑93 (MQ=255)
                                                            aCGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTAtgtg                             >  2:308958/1‑93 (MQ=255)
                                                              ggCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGca                           <  2:2306657/93‑1 (MQ=255)
                                                                cAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGcaca                         <  2:2589466/93‑1 (MQ=255)
                                                                 aaaTGCCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACaa                        <  2:2869481/93‑1 (MQ=255)
                                                                 aaaTACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACaa                        <  1:1942676/93‑1 (MQ=255)
                                                                 aaaTACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACaa                        >  1:2519543/1‑93 (MQ=255)
                                                                 aaaTACAACTGTGGTTTATGCCGGCCGCGACCAGAATGTACATGGTCACGCACTGGATACTACGCTGAACGGGGGGTACCAGTATGTCGACaa                        >  2:767226/1‑93 (MQ=255)
                                                                  aaTACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAAc                       >  1:445310/1‑93 (MQ=255)
                                                                  aaTACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAAc                       >  1:39735/1‑93 (MQ=255)
                                                                  aaTACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAAc                       >  2:2505620/1‑93 (MQ=255)
                                                                     aCCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAAcggc                    >  2:3269307/1‑93 (MQ=255)
                                                                      ccACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAAcggcg                   <  2:594503/93‑1 (MQ=255)
                                                                       cACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAAcggcgg                  >  1:4150441/1‑93 (MQ=255)
                                                                       cACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAAcggcgg                  >  1:3103251/1‑93 (MQ=255)
                                                                        aCTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGt                 >  2:2929168/1‑93 (MQ=255)
                                                                         cTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTa                >  2:3304824/1‑93 (MQ=255)
                                                                          tgtgGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGCGGATACCAGTATGTGCACAACGGCGGTAc               >  1:1912089/1‑93 (MQ=255)
                                                                           gtgGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACa              <  1:5803365/93‑1 (MQ=255)
                                                                           gtgGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACa              <  2:2646316/93‑1 (MQ=255)
                                                                           gtgGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACa              >  1:3673492/1‑93 (MQ=255)
                                                                             ggTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGc            >  1:2533088/1‑93 (MQ=255)
                                                                              gTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCg           >  2:6172779/1‑93 (MQ=255)
                                                                                ttATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTc         >  2:3379535/1‑93 (MQ=255)
                                                                                ttATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGAGTc         >  2:5652404/1‑93 (MQ=255)
                                                                                ttATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGGACAGCGTc         >  1:2246326/1‑93 (MQ=255)
                                                                                   tGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTgc      >  1:1028666/1‑92 (MQ=255)
                                                                                     ccGGCAGCGACCAGACTGTACATGGTCACGCACTGCATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGaca    <  2:2219192/93‑1 (MQ=255)
                                                                                       ggcagcGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGTATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACt  <  2:6346934/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGGGATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCGGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACT  >  NC_000913/2070185‑2070364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGGGGATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCGGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACTGTT  >  NC_000913/2070183‑2070367
                                                                                            |                                                                                            
GCGGGGATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAG                                                                                             <  SRR4302537.2164550/94‑1 (MQ=60)
 CGGGGATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTTAAGGAACGGCAAATACCACTGTTGTTTATGCTGGCAGC                                                                                            >  SRR4302537.6096713/1‑94 (MQ=60)
 CGGGGATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGC                                                                                            >  SRR4302537.6519524/1‑94 (MQ=60)
  GGGGATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCG                                                                                           <  SRR4302537.5143726/94‑1 (MQ=60)
  GGGGATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCG                                                                                           <  SRR4302537.2273558/94‑1 (MQ=60)
    GGATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGAC                                                                                         >  SRR4302537.1198952/1‑94 (MQ=60)
     GATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACC                                                                                        >  SRR4302537.4724/1‑94 (MQ=60)
      ATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCA                                                                                       <  SRR4302537.5052988/94‑1 (MQ=60)
      ATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCA                                                                                       >  SRR4302537.1943487/1‑94 (MQ=60)
          CGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACT                                                                                   >  SRR4302537.2345893/1‑94 (MQ=60)
          CGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACT                                                                                   <  SRR4302537.183753/94‑1 (MQ=60)
           GTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTG                                                                                  <  SRR4302537.6307493/94‑1 (MQ=60)
            TACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGT                                                                                 <  SRR4302537.1456975/94‑1 (MQ=60)
             ACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTA                                                                                >  SRR4302537.1740116/1‑94 (MQ=60)
              CGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTAC                                                                               <  SRR4302537.5448497/94‑1 (MQ=60)
              CGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTAC                                                                               <  SRR4302537.5335881/94‑1 (MQ=60)
                CACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACAT                                                                             <  SRR4302537.3687373/94‑1 (MQ=60)
                CACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACAT                                                                             >  SRR4302537.1642510/1‑94 (MQ=60)
                CACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACAT                                                                             <  SRR4302537.2819466/94‑1 (MQ=60)
                CACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACAT                                                                             <  SRR4302537.5136652/94‑1 (MQ=60)
                CACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGCCTATGCCGGCAGCGACCAGACTGTACAT                                                                             >  SRR4302537.911490/1‑94 (MQ=60)
                 ACAACCATCAATAAAAACGGTCGCCCGATTGTGAGAGCTGAAGGACCGGCAAATACCACTGTGGTTTATGCAGGCAGCGACCCGAATGTACATG                                                                            >  SRR4302537.1843721/1‑94 (MQ=60)
                  CAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGG                                                                           <  SRR4302537.1742413/94‑1 (MQ=60)
                    ACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTC                                                                         <  SRR4302537.687140/94‑1 (MQ=60)
                     CCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCA                                                                        <  SRR4302537.2906744/94‑1 (MQ=60)
                      CATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCAC                                                                       >  SRR4302537.1861449/1‑94 (MQ=60)
                      CATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCAC                                                                       <  SRR4302537.35095/94‑1 (MQ=60)
                      CATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCAC                                                                       <  SRR4302537.3282299/94‑1 (MQ=60)
                         CAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCA                                                                    >  SRR4302537.2396333/1‑94 (MQ=60)
                          AATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCAC                                                                   >  SRR4302537.712571/1‑94 (MQ=60)
                          AATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCAC                                                                   >  SRR4302537.4303469/1‑94 (MQ=60)
                           ATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACT                                                                  <  SRR4302537.6271824/94‑1 (MQ=60)
                            TAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTG                                                                 <  SRR4302537.4120437/94‑1 (MQ=60)
                             AAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGG                                                                <  SRR4302537.879513/94‑1 (MQ=60)
                             AAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGG                                                                <  SRR4302537.3451636/94‑1 (MQ=60)
                              AAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGA                                                               >  SRR4302537.5619888/1‑94 (MQ=60)
                               AAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGAT                                                              >  SRR4302537.5728484/1‑94 (MQ=60)
                                  CGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACC                                                           >  SRR4302537.4149994/1‑94 (MQ=60)
                                      CGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTTGATACCACGC                                                       >  SRR4302537.6307118/1‑94 (MQ=60)
                                        CCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTG                                                     <  SRR4302537.4227992/94‑1 (MQ=60)
                                         CAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGA                                                    >  SRR4302537.6314342/1‑94 (MQ=60)
                                         CAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTCGATACCACGCTGA                                                    >  SRR4302537.5618338/1‑94 (MQ=60)
                                          AGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAA                                                   >  SRR4302537.1049997/1‑94 (MQ=60)
                                           GATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAAT                                                  <  SRR4302537.5872768/94‑1 (MQ=60)
                                           GATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAAT                                                  <  SRR4302537.3014999/94‑1 (MQ=60)
                                            ATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATG                                                 >  SRR4302537.4047751/1‑94 (MQ=60)
                                             TTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATTCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGG                                                >  SRR4302537.1704613/1‑94 (MQ=60)
                                              TGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGG                                               <  SRR4302537.5711088/94‑1 (MQ=60)
                                              TGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGG                                               <  SRR4302537.5941267/94‑1 (MQ=60)
                                               GTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGG                                              >  SRR4302537.3622926/1‑94 (MQ=60)
                                               GTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGG                                              >  SRR4302537.853581/1‑94 (MQ=60)
                                                TGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGG                                             <  SRR4302537.1337343/94‑1 (MQ=60)
                                                  AGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGAT                                           >  SRR4302537.936653/1‑94 (MQ=60)
                                                    AGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATAC                                         <  SRR4302537.240461/94‑1 (MQ=60)
                                                     GCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACC                                        <  SRR4302537.2878017/94‑1 (MQ=60)
                                                     GCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACC                                        >  SRR4302537.410491/1‑94 (MQ=60)
                                                      CTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCA                                       <  SRR4302537.404155/94‑1 (MQ=60)
                                                       TGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAG                                      <  SRR4302537.3475799/94‑1 (MQ=60)
                                                        GAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGT                                     <  SRR4302537.2427343/94‑1 (MQ=60)
                                                        GAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGT                                     <  SRR4302537.6393003/94‑1 (MQ=60)
                                                         AAGGAACGGCAAATACCACTGTGGTTTATGCCGGAAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTA                                    >  SRR4302537.1489768/1‑94 (MQ=60)
                                                          AGGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTAT                                   >  SRR4302537.4816256/1‑94 (MQ=60)
                                                           GGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATG                                  <  SRR4302537.2537886/94‑1 (MQ=60)
                                                           GGAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATG                                  <  SRR4302537.124063/94‑1 (MQ=60)
                                                            GAACGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGT                                 <  SRR4302537.104972/94‑1 (MQ=60)
                                                               CGGCAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCA                              >  SRR4302537.4928247/1‑94 (MQ=60)
                                                                  CAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAA                           >  SRR4302537.2537238/1‑94 (MQ=60)
                                                                  CAAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAA                           <  SRR4302537.3204322/94‑1 (MQ=60)
                                                                   AAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAAC                          >  SRR4302537.448400/1‑94 (MQ=60)
                                                                   AAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAAC                          <  SRR4302537.1956180/94‑1 (MQ=60)
                                                                   AAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAAC                          >  SRR4302537.40035/1‑94 (MQ=60)
                                                                   AAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAAC                          <  SRR4302537.3761561/94‑1 (MQ=60)
                                                                   AAATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAAC                          <  SRR4302537.3558580/94‑1 (MQ=60)
                                                                    AATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACG                         <  SRR4302537.990304/94‑1 (MQ=60)
                                                                    AATACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACG                         <  SRR4302537.3319655/94‑1 (MQ=60)
                                                                      TACCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATTGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGC                       >  SRR4302537.4122907/1‑94 (MQ=60)
                                                                        CCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGG                     >  SRR4302537.4179417/1‑94 (MQ=60)
                                                                        CCACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGG                     >  SRR4302537.3124922/1‑94 (MQ=60)
                                                                         CACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGA                    >  SRR4302537.1836490/1‑94 (MQ=60)
                                                                         CACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGA                    >  SRR4302537.1841791/1‑94 (MQ=60)
                                                                         CACTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACGGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGT                    <  SRR4302537.4221102/94‑1 (MQ=60)
                                                                           CTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTAC                  >  SRR4302537.2781611/1‑94 (MQ=60)
                                                                           CTGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGCGGATACCAGTATGTGCACAACGGCGGTAC                  >  SRR4302537.1925361/1‑94 (MQ=60)
                                                                            TGTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACA                 >  SRR4302537.3699335/1‑94 (MQ=60)
                                                                             GTGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAG                <  SRR4302537.5843161/94‑1 (MQ=60)
                                                                              TGGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGC               >  SRR4302537.2550861/1‑94 (MQ=60)
                                                                               GGTTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCG              <  SRR4302537.2107043/94‑1 (MQ=60)
                                                                                 TTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTC            <  SRR4302537.5226368/94‑1 (MQ=60)
                                                                                 TTTATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGGACAGCGTC            >  SRR4302537.2262177/1‑94 (MQ=60)
                                                                                    ATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGC         >  SRR4302537.1035748/1‑94 (MQ=60)
                                                                                    ATGCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGA         >  SRR4302537.168436/1‑94 (MQ=60)
                                                                                      GCCGGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACA       >  SRR4302537.4168015/1‑94 (MQ=60)
                                                                                         GGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACTG    >  SRR4302537.3951197/1‑94 (MQ=60)
                                                                                         GGCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACTG    >  SRR4302537.5021076/1‑94 (MQ=60)
                                                                                          GCAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACTGT   <  SRR4302537.3422375/94‑1 (MQ=60)
                                                                                           CAGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACTGTT  <  SRR4302537.5536323/94‑1 (MQ=60)
                                                                                            |                                                                                            
GCGGGGATGCCGTACGCACAACCATCAATAAAAACGGTCGCCAGATTGTGAGAGCTGAAGGAACGGCAAATACCACTGTGGTTTATGCCGGCGGCGACCAGACTGTACATGGTCACGCACTGGATACCACGCTGAATGGGGGATACCAGTATGTGCACAACGGCGGTACAGCGTCTGACACTGTT  >  NC_000913/2070183‑2070367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑