Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,087,648 T→C T39A (ACT→GCT)  yefM ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,087,6480TC96.4% 721.7 / 10.2 221T39A (ACT→GCT) yefMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (5/3);  new base C (96/116);  total (102/119)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 233 total aligned reads displayed.

GTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGT  >  NC_000913/2087556‑2087737
                                                                                            |                                                                                         
   aGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGcaat                                                                                        >  2:551897/1‑93 (MQ=255)
     cGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGACCACAAGCCTCTCCATTCTGGCGAGcaataa                                                                                      <  2:4570128/93‑1 (MQ=255)
     cGGGGGAGCGCAGTAGATAAGCCGTCTCTGCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGcaataa                                                                                      <  2:4349493/93‑1 (MQ=255)
       ggggAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAgg                                                                                    <  2:236959/93‑1 (MQ=255)
        gggAGCGCAGTAGATAAGGCGTATCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGa                                                                                   >  2:797250/1‑93 (MQ=255)
        gggAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGa                                                                                   <  2:3943528/93‑1 (MQ=255)
        gggAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGAAATAAGGa                                                                                   >  1:4809089/1‑93 (MQ=255)
         ggAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGAt                                                                                  <  1:887062/93‑1 (MQ=255)
         ggAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGAt                                                                                  >  2:5926296/1‑93 (MQ=255)
          gAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATc                                                                                 <  1:3778565/93‑1 (MQ=255)
           aGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCg                                                                                <  2:1286571/93‑1 (MQ=255)
           aGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCg                                                                                <  1:4652282/93‑1 (MQ=255)
            gcgcAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCgg                                                                               >  1:1612708/1‑93 (MQ=255)
             cgcAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCggg                                                                              <  2:4910582/93‑1 (MQ=255)
              gcAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCgggg                                                                             <  2:2149913/93‑1 (MQ=255)
              gcAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCgggg                                                                             >  1:4181748/1‑93 (MQ=255)
               cAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGc                                                                            >  2:6180297/1‑93 (MQ=255)
               cAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGc                                                                            >  1:3540466/1‑93 (MQ=255)
                aGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCa                                                                           >  1:4118250/1‑93 (MQ=255)
                 gTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCAt                                                                          >  2:2170499/1‑93 (MQ=255)
                  tAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATg                                                                         >  1:1665935/1‑93 (MQ=255)
                  tAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATg                                                                         >  1:3910109/1‑93 (MQ=255)
                   aGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGa                                                                        <  2:4215540/93‑1 (MQ=255)
                   aGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGa                                                                        <  2:2651157/93‑1 (MQ=255)
                   aGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGa                                                                        <  2:4374176/93‑1 (MQ=255)
                    gATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGAt                                                                       <  1:2547912/93‑1 (MQ=255)
                      tAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCt                                                                     <  2:3889831/93‑1 (MQ=255)
                        aGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTc                                                                   <  2:5127998/93‑1 (MQ=255)
                          ccGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCaa                                                                 <  1:4153228/93‑1 (MQ=255)
                           cGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAAc                                                                <  1:6448080/93‑1 (MQ=255)
                           cGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCAGACGAGCAATAAGGATCGGGGCATGATCTTCCAc                                                                >  2:1682211/1‑93 (MQ=255)
                            gTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACg                                                               >  2:4663538/1‑93 (MQ=255)
                              ttctTCCGGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGc                                                             <  1:2778618/92‑1 (MQ=255)
                              ctctTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGc                                                             >  1:525873/1‑93 (MQ=255)
                               tctTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCt                                                            >  1:3300408/1‑93 (MQ=255)
                               tctTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCt                                                            >  2:1967681/1‑93 (MQ=255)
                                ctTCCAGCGAGTTGTATTCTGCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCtt                                                           <  1:2061428/93‑1 (MQ=255)
                                 ttCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCttt                                                          >  1:1972113/1‑93 (MQ=255)
                                  tCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTtc                                                         <  2:470738/93‑1 (MQ=255)
                                   ccAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTtca                                                        <  1:3146986/93‑1 (MQ=255)
                                      gCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca                                                     <  1:5016092/93‑1 (MQ=255)
                                      gCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca                                                     <  1:1408422/93‑1 (MQ=255)
                                      gCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca                                                     >  2:1516/1‑93 (MQ=255)
                                      gCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca                                                     <  2:685078/93‑1 (MQ=255)
                                      gCGAGTTGTATTCTTCGAGTAACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca                                                     <  1:4688380/93‑1 (MQ=255)
                                       cGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTcatcat                                                    <  2:2456806/93‑1 (MQ=255)
                                        gAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAtt                                                   >  1:4975624/1‑93 (MQ=255)
                                        gAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAtt                                                   >  1:1063223/1‑93 (MQ=255)
                                        gAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAtt                                                   >  2:1528708/1‑93 (MQ=255)
                                         aGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg                                                  <  1:6006615/93‑1 (MQ=255)
                                         aGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg                                                  >  2:3438948/1‑93 (MQ=255)
                                          gTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgt                                                 <  1:5088605/93‑1 (MQ=255)
                                          gTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgt                                                 <  2:6046405/93‑1 (MQ=255)
                                          gTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgt                                                 >  1:4262690/1‑93 (MQ=255)
                                            tGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgttg                                               >  1:1456226/1‑93 (MQ=255)
                                              tATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGcc                                             >  2:2588842/1‑93 (MQ=255)
                                                 tcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGAc                                          <  1:5833416/93‑1 (MQ=255)
                                                    tggggTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACaaa                                       <  1:5951404/89‑1 (MQ=255)
                                                    tcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACaaa                                       <  2:3329297/93‑1 (MQ=255)
                                                    tcGAGTGACATAAGAACACAAGCCTCACCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACaaa                                       <  1:4413767/93‑1 (MQ=255)
                                                         ggACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTg                                  <  1:1807292/92‑1 (MQ=255)
                                                          gACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGa                                 <  2:3607130/93‑1 (MQ=255)
                                                           aCATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGAc                                <  1:5627327/93‑1 (MQ=255)
                                                           aCATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGAc                                <  1:4338803/93‑1 (MQ=255)
                                                           aCATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGAc                                <  2:308483/93‑1 (MQ=255)
                                                            cATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGAcg                               >  1:6102095/1‑93 (MQ=255)
                                                                aGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCt                           >  1:3866249/1‑93 (MQ=255)
                                                                 gAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGTGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCtt                          >  2:5260937/1‑93 (MQ=255)
                                                                  aaCACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTc                         >  1:2487486/1‑93 (MQ=255)
                                                                  aaCACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTc                         <  1:3592391/93‑1 (MQ=255)
                                                                  aaCACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTc                         >  2:2806205/1‑93 (MQ=255)
                                                                   acacAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCg                        >  1:2846459/1‑93 (MQ=255)
                                                                   acacAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCg                        >  1:724472/1‑93 (MQ=255)
                                                                     acaAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCt                      >  1:493557/1‑93 (MQ=255)
                                                                     acaAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCt                      <  2:6435948/93‑1 (MQ=255)
                                                                     acaAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCt                      >  1:5672102/1‑93 (MQ=255)
                                                                     acaAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCt                      >  1:3032291/1‑93 (MQ=255)
                                                                     acaAGCCTCTCCATTCTGAAGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCt                      >  2:6292217/1‑93 (MQ=255)
                                                                      caAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTg                     <  1:2644860/93‑1 (MQ=255)
                                                                      caAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTg                     <  2:1383327/93‑1 (MQ=255)
                                                                       aaGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGt                    >  1:1239161/1‑93 (MQ=255)
                                                                        aGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTa                   <  2:3102183/93‑1 (MQ=255)
                                                                         gCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAg                  >  2:4155612/1‑93 (MQ=255)
                                                                          ccTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGc                 <  2:1700603/93‑1 (MQ=255)
                                                                           ctctCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCt                >  2:621511/1‑93 (MQ=255)
                                                                           ctctCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCt                >  1:3201504/1‑93 (MQ=255)
                                                                           ctctCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCt                >  2:4789766/1‑93 (MQ=255)
                                                                             ctcCATTCTGACGAGCAAGAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTaa              <  2:3014843/93‑1 (MQ=255)
                                                                              tcCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAAt             <  2:3574639/93‑1 (MQ=255)
                                                                              tcCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAAt             >  1:4079651/1‑93 (MQ=255)
                                                                              tcCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAAt             >  1:908019/1‑93 (MQ=255)
                                                                                 aTTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGt          >  2:1324441/1‑93 (MQ=255)
                                                                                  ttCTTACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTa         <  1:5102836/93‑1 (MQ=255)
                                                                                  ttCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTa         >  2:2788596/1‑93 (MQ=255)
                                                                                      gACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCa     <  2:3311799/93‑1 (MQ=255)
                                                                                       aCGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATGATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCAt    >  1:3458132/1‑93 (MQ=255)
                                                                                        cGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATg   >  2:1100528/1‑93 (MQ=255)
                                                                                        cGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATg   <  1:210116/93‑1 (MQ=255)
                                                                                         gAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGt  <  2:2205007/93‑1 (MQ=255)
                                                                                         gAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGt  <  1:5321863/93‑1 (MQ=255)
                                                                                            |                                                                                         
GTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGT  >  NC_000913/2087556‑2087737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAAT  >  NC_000913/2087555‑2087741
                                                                                             |                                                                                             
CGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGC                                                                                               <  SRR4302537.5590566/94‑1 (MQ=60)
CGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGC                                                                                               >  SRR4302537.2465012/1‑94 (MQ=60)
 GTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTA                                                                                              <  SRR4302537.6066710/94‑1 (MQ=60)
 GTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCA                                                                                              >  SRR4302537.2067931/1‑94 (MQ=60)
 GTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCA                                                                                              >  SRR4302537.1644899/1‑94 (MQ=60)
   TAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAAT                                                                                            >  SRR4302537.93365/1‑94 (MQ=60)
    AGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATA                                                                                           >  SRR4302537.1088982/1‑94 (MQ=60)
    AGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATA                                                                                           <  SRR4302537.3150194/94‑1 (MQ=60)
      GGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAG                                                                                         <  SRR4302537.5319316/94‑1 (MQ=60)
      CGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAG                                                                                         >  SRR4302537.5800903/1‑94 (MQ=60)
      CGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAG                                                                                         >  SRR4302537.279383/1‑94 (MQ=60)
       GGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGG                                                                                        <  SRR4302537.1424095/94‑1 (MQ=60)
        GGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGAAATAAGGA                                                                                       >  SRR4302537.4842283/1‑94 (MQ=60)
         GGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGAT                                                                                      <  SRR4302537.600964/94‑1 (MQ=60)
         GGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGAT                                                                                      <  SRR4302537.2130388/94‑1 (MQ=60)
          GGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATC                                                                                     <  SRR4302537.893165/94‑1 (MQ=60)
           GAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCG                                                                                    <  SRR4302537.3805099/94‑1 (MQ=60)
            AGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGG                                                                                   >  SRR4302537.1623980/1‑94 (MQ=60)
            AGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGG                                                                                   <  SRR4302537.4684448/94‑1 (MQ=60)
              CGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGG                                                                                 >  SRR4302537.4210941/1‑94 (MQ=60)
              CGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGG                                                                                 >  SRR4302537.416990/1‑94 (MQ=60)
               GCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGC                                                                                >  SRR4302537.5532490/1‑94 (MQ=60)
               GCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGC                                                                                >  SRR4302537.3565474/1‑94 (MQ=60)
                CAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCA                                                                               >  SRR4302537.4147010/1‑94 (MQ=60)
                  GTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATG                                                                             >  SRR4302537.1677608/1‑94 (MQ=60)
                  GTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATG                                                                             >  SRR4302537.3937443/1‑94 (MQ=60)
                   ctgaatatccaccagcagtttcattccgtgTTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGA                                                                            >  SRR4302537.5345315/31‑94 (MQ=60)
                   TAGATAAGCCGTCTCTTCCAGCGAGTTGTATCCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGA                                                                            <  SRR4302537.504658/94‑1 (MQ=60)
                    AGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGAT                                                                           <  SRR4302537.4124845/94‑1 (MQ=60)
                    AGAGAAGCCTCCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGAT                                                                           <  SRR4302537.5010843/94‑1 (MQ=60)
                     GATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATC                                                                          <  SRR4302537.2565759/94‑1 (MQ=60)
                     GATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCCAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATC                                                                          >  SRR4302537.5911717/1‑94 (MQ=60)
                        AAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTC                                                                       <  SRR4302537.4821435/94‑1 (MQ=60)
                         AGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCA                                                                      <  SRR4302537.5709563/94‑1 (MQ=60)
                           CCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAAC                                                                    <  SRR4302537.4182228/94‑1 (MQ=60)
                           CCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAAC                                                                    >  SRR4302537.1987059/1‑94 (MQ=60)
                           CCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAAC                                                                    <  SRR4302537.4758159/94‑1 (MQ=60)
                            CGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACG                                                                   <  SRR4302537.6491819/94‑1 (MQ=60)
                            CGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACG                                                                   >  SRR4302537.2937934/1‑94 (MQ=60)
                             GTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGG                                                                  >  SRR4302537.4258473/1‑94 (MQ=60)
                             GTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGG                                                                  <  SRR4302537.4939320/94‑1 (MQ=60)
                              TCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGC                                                                 >  SRR4302537.529479/1‑94 (MQ=60)
                               TTCTTCCGGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCT                                                                <  SRR4302537.2798049/94‑1 (MQ=60)
                               CTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCT                                                                >  SRR4302537.3323744/1‑94 (MQ=60)
                                 CTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTT                                                              >  SRR4302537.1985825/1‑94 (MQ=60)
                                 CTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCGGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTT                                                              <  SRR4302537.4701086/94‑1 (MQ=60)
                                 CTTCCAGCGAGTTGTATTCTGCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTT                                                              <  SRR4302537.2075907/94‑1 (MQ=60)
                                 CTCCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTT                                                              <  SRR4302537.3816883/94‑1 (MQ=60)
                                  TTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTC                                                             >  SRR4302537.711429/1‑94 (MQ=60)
                                   TCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCA                                                            <  SRR4302537.3564076/94‑1 (MQ=60)
                                    CCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCAT                                                           <  SRR4302537.3169063/94‑1 (MQ=60)
                                      AGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCA                                                         <  SRR4302537.1735656/94‑1 (MQ=60)
                                      AGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCA                                                         >  SRR4302537.3302065/1‑94 (MQ=60)
                                      AGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCA                                                         >  SRR4302537.1608461/1‑94 (MQ=60)
                                       GCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAT                                                        <  SRR4302537.1418232/94‑1 (MQ=60)
                                       GCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAT                                                        <  SRR4302537.5050636/94‑1 (MQ=60)
                                       GCGAGTTGTATTCTTCGAGTAACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAT                                                        <  SRR4302537.4720813/94‑1 (MQ=60)
                                        CGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATT                                                       >  SRR4302537.5009920/1‑94 (MQ=60)
                                        CGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATT                                                       >  SRR4302537.1070551/1‑94 (MQ=60)
                                         GAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTG                                                      <  SRR4302537.3463473/94‑1 (MQ=60)
                                          AGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGT                                                     >  SRR4302537.4292310/1‑94 (MQ=60)
                                          AGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGT                                                     <  SRR4302537.6047884/94‑1 (MQ=60)
                                           GTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTT                                                    <  SRR4302537.5123636/94‑1 (MQ=60)
                                            TTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTG                                                   <  SRR4302537.5097243/94‑1 (MQ=60)
                                            TTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTG                                                   >  SRR4302537.1466368/1‑94 (MQ=60)
                                               TATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCG                                                >  SRR4302537.1834325/1‑94 (MQ=60)
                                                 TTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGAC                                              <  SRR4302537.3922231/94‑1 (MQ=60)
                                                  TCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACA                                             <  SRR4302537.5873396/94‑1 (MQ=60)
                                                     tgggGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAAT                                          <  SRR4302537.5992258/90‑1 (MQ=60)
                                                     tcgaaggcccTCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAAT                                          <  SRR4302537.3648447/84‑1 (MQ=60)
                                                     TCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAAT                                          >  SRR4302537.2551337/1‑94 (MQ=60)
                                                     TCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAAT                                          <  SRR4302537.5167473/94‑1 (MQ=60)
                                                     TCGAGTGACATAAGAACACAAGCCTCACCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAAT                                          <  SRR4302537.4444230/94‑1 (MQ=60)
                                                        AGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCT                                       <  SRR4302537.4204791/94‑1 (MQ=60)
                                                          TGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGA                                     >  SRR4302537.4556436/1‑94 (MQ=60)
                                                          GGACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGA                                     <  SRR4302537.1819901/94‑1 (MQ=60)
                                                            ACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACG                                   <  SRR4302537.5665824/94‑1 (MQ=60)
                                                            ACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACG                                   >  SRR4302537.6144021/1‑94 (MQ=60)
                                                            ACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACG                                   <  SRR4302537.4400926/94‑1 (MQ=60)
                                                            ACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACG                                   <  SRR4302537.4368914/94‑1 (MQ=60)
                                                            ACATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACG                                   <  SRR4302537.3642337/94‑1 (MQ=60)
                                                              ATCAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCG                                 >  SRR4302537.5135330/1‑94 (MQ=60)
                                                                CAGAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCT                               >  SRR4302537.3893308/1‑94 (MQ=60)
                                                                  GAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTC                             <  SRR4302537.436402/94‑1 (MQ=60)
                                                                  GAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTC                             >  SRR4302537.2505003/1‑94 (MQ=60)
                                                                  AAACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTC                             <  SRR4302537.2579336/94‑1 (MQ=60)
                                                                   AACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCG                            >  SRR4302537.729417/1‑94 (MQ=60)
                                                                   AACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCG                            >  SRR4302537.2866386/1‑94 (MQ=60)
                                                                   AACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCG                            <  SRR4302537.3617714/94‑1 (MQ=60)
                                                                    ACACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGC                           >  SRR4302537.1857629/1‑94 (MQ=60)
                                                                     CGCAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCT                          <  SRR4302537.1545144/94‑1 (MQ=60)
                                                                     CACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCT                          >  SRR4302537.4376330/1‑94 (MQ=60)
                                                                     CACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCT                          >  SRR4302537.3053434/1‑94 (MQ=60)
                                                                     CACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCT                          >  SRR4302537.5710913/1‑94 (MQ=60)
                                                                     CACAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCT                          >  SRR4302537.496958/1‑94 (MQ=60)
                                                                       CAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGT                        >  SRR4302537.1247777/1‑94 (MQ=60)
                                                                       CAAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGT                        <  SRR4302537.2663358/94‑1 (MQ=60)
                                                                        AAGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTA                       >  SRR4302537.87681/1‑94 (MQ=60)
                                                                         AGCCTCTCCATTCTGACGAGCAATAAGGATCGTGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAG                      <  SRR4302537.2856848/94‑1 (MQ=60)
                                                                         AGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAG                      <  SRR4302537.4025365/94‑1 (MQ=60)
                                                                         AGCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAG                      <  SRR4302537.1345116/94‑1 (MQ=60)
                                                                          GCCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTGAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGC                     >  SRR4302537.3473061/1‑94 (MQ=60)
                                                                           CCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCT                    <  SRR4302537.6003842/94‑1 (MQ=60)
                                                                           CCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCT                    <  SRR4302537.2462045/94‑1 (MQ=60)
                                                                           CCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCT                    <  SRR4302537.2114517/94‑1 (MQ=60)
                                                                           CCTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCT                    >  SRR4302537.3224013/1‑94 (MQ=60)
                                                                            CTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTA                   <  SRR4302537.4023415/94‑1 (MQ=60)
                                                                            CTCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTA                   >  SRR4302537.2665551/1‑94 (MQ=60)
                                                                             TCTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAA                  >  SRR4302537.3073640/1‑94 (MQ=60)
                                                                              CTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAAT                 >  SRR4302537.4108133/1‑94 (MQ=60)
                                                                              CTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAAT                 >  SRR4302537.914294/1‑94 (MQ=60)
                                                                              CTCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAAT                 >  SRR4302537.570621/1‑94 (MQ=60)
                                                                               TCCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATT                >  SRR4302537.1633890/1‑94 (MQ=60)
                                                                                CCATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTG               >  SRR4302537.3199721/1‑94 (MQ=60)
                                                                                 CATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGT              >  SRR4302537.6509189/1‑94 (MQ=60)
                                                                                  ATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTA             >  SRR4302537.4183651/1‑94 (MQ=60)
                                                                                  ATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTA             <  SRR4302537.5243423/94‑1 (MQ=60)
                                                                                  ATTCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTA             >  SRR4302537.4973850/1‑94 (MQ=60)
                                                                                   TTCTTACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTAC            <  SRR4302537.5138004/94‑1 (MQ=60)
                                                                                    TCTGACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACG           <  SRR4302537.6167227/94‑1 (MQ=60)
                                                                                       GACGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATGATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCAT        >  SRR4302537.3482582/1‑94 (MQ=60)
                                                                                        ACGAGTAATAAGGATCGGGGCATGATCTTCATCGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATG       <  SRR4302537.3568382/94‑1 (MQ=60)
                                                                                         CGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGT      <  SRR4302537.211624/94‑1 (MQ=60)
                                                                                         CGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGT      <  SRR4302537.905573/94‑1 (MQ=60)
                                                                                         CGAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGT      >  SRR4302537.3695100/1‑94 (MQ=60)
                                                                                          GAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTC     <  SRR4302537.5358388/94‑1 (MQ=60)
                                                                                          GAGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTC     >  SRR4302537.5646886/1‑94 (MQ=60)
                                                                                           AGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCA    >  SRR4302537.6259198/1‑94 (MQ=60)
                                                                                           AGCAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCA    >  SRR4302537.4439411/1‑94 (MQ=60)
                                                                                             CAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAAT  <  SRR4302537.1880661/94‑1 (MQ=60)
                                                                                             CAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAAT  >  SRR4302537.5160409/1‑94 (MQ=60)
                                                                                             CAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAAT  <  SRR4302537.4640312/94‑1 (MQ=60)
                                                                                             CAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAAT  >  SRR4302537.3446055/1‑94 (MQ=60)
                                                                                             CAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAAT  >  SRR4302537.5591994/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAAT  >  NC_000913/2087555‑2087741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑