Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,103,209	T→C	M291V (ATG→GTG)	wbbI ←	putative Galf transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,103,209	0	T	C	95.2%	812.8 / 19.7	252	M291V (ATG→GTG)	wbbI	putative Galf transferase
Reads supporting (aligned to +/- strand): ref base T (7/5); new base C (128/112); total (135/117)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 253 total aligned reads displayed.

CAAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTTTA  >  NC_000913/2103119‑2103297

cAAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACgg                                                                                        >  2:6032234/1‑93 (MQ=255)

 aaGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGa                                                                                       >  2:886168/1‑93 (MQ=255)

 aaGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGa                                                                                       <  1:958626/93‑1 (MQ=255)

 aaGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGa                                                                                       <  2:3367072/93‑1 (MQ=255)

  aGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAg                                                                                      >  1:200608/1‑93 (MQ=255)

   gAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGt                                                                                     >  2:1275230/1‑93 (MQ=255)

    aaCATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTc                                                                                    <  1:5922099/93‑1 (MQ=255)

    aaCATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTc                                                                                    <  2:1324658/93‑1 (MQ=255)

    aaCATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTc                                                                                    >  2:5490072/1‑93 (MQ=255)

      cATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTcaa                                                                                  >  1:6405803/1‑93 (MQ=255)

      cATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTcaa                                                                                  <  1:3982531/93‑1 (MQ=255)

      cATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTcaa                                                                                  <  2:3271493/93‑1 (MQ=255)

       aTCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTcaac                                                                                 <  1:2027575/93‑1 (MQ=255)

          ccTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAAt                                                                              >  1:1233722/1‑93 (MQ=255)

          ccTGAAGTAACTTCCTGTTCGAATTCTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAAt                                                                              >  2:431081/1‑93 (MQ=255)

           cTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAAtc                                                                             <  1:2533857/93‑1 (MQ=255)

            tGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAAtct                                                                            >  2:4995103/1‑93 (MQ=255)

              aaGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATctct                                                                          <  2:2443092/93‑1 (MQ=255)

              aaGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATctct                                                                          <  2:6053481/93‑1 (MQ=255)

              aaGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATctct                                                                          >  2:1789005/1‑93 (MQ=255)

                  aaCTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCa                                                                      >  2:5583328/1‑93 (MQ=255)

                  aaCTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCa                                                                      >  2:4185203/1‑93 (MQ=255)

                   aCTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCAt                                                                     >  2:1754277/1‑93 (MQ=255)

                    cTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCAtt                                                                    <  2:1659524/93‑1 (MQ=255)

                      tCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTc                                                                  <  2:2383605/93‑1 (MQ=255)

                         tGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCttt                                                               <  2:3928869/93‑1 (MQ=255)

                           ttCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTttga                                                             <  2:3206680/93‑1 (MQ=255)

                           ttCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTttga                                                             >  2:5986102/1‑93 (MQ=255)

                             cGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTttgatt                                                           <  1:2812659/93‑1 (MQ=255)

                             cGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTttgatt                                                           <  2:5880243/93‑1 (MQ=255)

                              gAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTttgattg                                                          <  1:6470144/93‑1 (MQ=255)

                              gAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTttgattg                                                          <  1:5157451/93‑1 (MQ=255)

                               aaTTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTttgattga                                                         >  1:3071459/1‑93 (MQ=255)

                                 ttttCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATc                                                       <  1:3180990/93‑1 (MQ=255)

                                 ttttCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATc                                                       >  1:4757935/1‑93 (MQ=255)

                                  tttCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATcc                                                      <  1:10823/93‑1 (MQ=255)

                                  tttCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATcc                                                      >  1:2781513/1‑93 (MQ=255)

                                   ttCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATccc                                                     >  2:1700480/1‑93 (MQ=255)

                                   ttCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATccc                                                     <  2:6168547/93‑1 (MQ=255)

                                   ttCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATccc                                                     >  2:6396836/1‑93 (MQ=255)

                                    tCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCa                                                    <  2:2824406/93‑1 (MQ=255)

                                     cTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCAc                                                   <  2:3135253/93‑1 (MQ=255)

                                      tGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACt                                                  >  1:1800900/1‑93 (MQ=255)

                                       gagaAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTg                                                 <  2:4819076/93‑1 (MQ=255)

                                          aaaTAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCat                                              >  2:2679552/1‑93 (MQ=255)

                                          aaaTAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCat                                              >  1:4205495/1‑93 (MQ=255)

                                           aataatTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCata                                             >  1:1789686/1‑93 (MQ=255)

                                             taatTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATc                                           >  1:4912691/1‑93 (MQ=255)

                                              aatTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATcc                                          >  2:5420668/1‑93 (MQ=255)

                                                tttttGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCcta                                        <  2:3453013/93‑1 (MQ=255)

                                                   ttGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCctattc                                     <  1:6273566/93‑1 (MQ=255)

                                                   ttGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCctattc                                     <  2:5091461/93‑1 (MQ=255)

                                                     gTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCctattcta                                   <  1:4566613/93‑1 (MQ=255)

                                                     gTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCctattcta                                   >  1:295584/1‑93 (MQ=255)

                                                      tATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCctattctat                                  <  1:342104/93‑1 (MQ=255)

                                                      tATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCctattctat                                  >  1:5361740/1‑93 (MQ=255)

                                                      tATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCctattctat                                  >  1:5049207/1‑93 (MQ=255)

                                                       aTTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCctattctatt                                 >  2:3410778/1‑93 (MQ=255)

                                                        ttCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCtatta                                <  1:2620858/93‑1 (MQ=255)

                                                        ttCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCtatta                                >  1:5591398/1‑93 (MQ=255)

                                                         tctcACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCtattat                               <  2:2899234/93‑1 (MQ=255)

                                                         gctcACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCtattat                               >  1:6094694/2‑93 (MQ=255)

                                                          ctcACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATc                              <  2:6274944/93‑1 (MQ=255)

                                                           tcACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCt                             >  1:5410831/1‑93 (MQ=255)

                                                            cACTAATTTGCTTATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTa                            <  2:798467/93‑1 (MQ=255)

                                                            cACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTa                            <  1:1014902/93‑1 (MQ=255)

                                                             aCTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTAc                           <  1:2290114/93‑1 (MQ=255)

                                                              cTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACa                          >  2:3024733/1‑93 (MQ=255)

                                                               tAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACaa                         >  1:5550971/1‑93 (MQ=255)

                                                               tAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACaa                         <  2:5314406/93‑1 (MQ=255)

                                                               tAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACaa                         <  2:4493673/93‑1 (MQ=255)

                                                                  tttGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGa                      <  1:366081/93‑1 (MQ=255)

                                                                  tttGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGa                      >  1:1599555/1‑93 (MQ=255)

                                                                  tttGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGa                      >  1:4298060/1‑93 (MQ=255)

                                                                  tttGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGAATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGa                      <  1:5485318/93‑1 (MQ=255)

                                                                   ttGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGaa                     >  1:2373320/1‑93 (MQ=255)

                                                                    tGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGaaa                    <  1:4958317/93‑1 (MQ=255)

                                                                      cTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATc                  <  2:5047917/93‑1 (MQ=255)

                                                                       ttATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATcc                 <  2:3544355/93‑1 (MQ=255)

                                                                       ttATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATcc                 <  1:3976467/93‑1 (MQ=255)

                                                                       ttATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATcc                 >  2:1496827/1‑93 (MQ=255)

                                                                        tataAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCg                >  1:4470152/1‑93 (MQ=255)

                                                                          taAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCa              >  2:4959462/1‑93 (MQ=255)

                                                                          taAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCa              >  2:1979812/1‑93 (MQ=255)

                                                                          taAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCa              >  1:4216815/1‑93 (MQ=255)

                                                                          taAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCa              >  2:5822002/1‑93 (MQ=255)

                                                                            aGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAg            <  1:6150227/93‑1 (MQ=255)

                                                                            aGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAg            <  2:5906458/93‑1 (MQ=255)

                                                                            aGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAg            <  2:2157650/93‑1 (MQ=255)

                                                                             gTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAgg           <  2:5673414/93‑1 (MQ=255)

                                                                             gTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAgg           <  1:3239796/93‑1 (MQ=255)

                                                                             gTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAgg           <  1:3637405/93‑1 (MQ=255)

                                                                              tttCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAggg          >  2:2226230/1‑93 (MQ=255)

                                                                                tCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGggcg        >  2:1066434/1‑93 (MQ=255)

                                                                                tCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGggcg        >  2:3988725/1‑93 (MQ=255)

                                                                                tCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGggcg        <  2:4201410/93‑1 (MQ=255)

                                                                                 cTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGggcgg       >  2:1933532/1‑93 (MQ=255)

                                                                                 cTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGggcgg       <  2:461644/93‑1 (MQ=255)

                                                                                  tATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGggcggc      >  1:3338874/1‑93 (MQ=255)

                                                                                   aTTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCt     <  1:3560061/93‑1 (MQ=255)

CAAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTTTA  >  NC_000913/2103119‑2103297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTCAAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTTTATCCCA  >  NC_000913/2103116‑2103302

CTTCAAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCAC                                                                                               >  SRR4302537.4327297/1‑94 (MQ=60)

 TTCAAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACG                                                                                              <  SRR4302537.5583958/94‑1 (MQ=60)

 TTCAAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACG                                                                                              >  SRR4302537.2386833/1‑94 (MQ=60)

  TCAAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGG                                                                                             <  SRR4302537.3194538/94‑1 (MQ=60)

   CAAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGA                                                                                            >  SRR4302537.3681836/1‑94 (MQ=60)

    AAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAG                                                                                           <  SRR4302537.1083937/94‑1 (MQ=60)

    AAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAG                                                                                           <  SRR4302537.965238/94‑1 (MQ=60)

    AAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAG                                                                                           <  SRR4302537.4239377/94‑1 (MQ=60)

    AAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAG                                                                                           >  SRR4302537.202045/1‑94 (MQ=60)

    AAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTCTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAG                                                                                           <  SRR4302537.3481467/94‑1 (MQ=60)

      GAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTC                                                                                         <  SRR4302537.1149391/94‑1 (MQ=60)

       AACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCA                                                                                        >  SRR4302537.5725189/1‑94 (MQ=60)

       AACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCA                                                                                        >  SRR4302537.5408659/1‑94 (MQ=60)

       AACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCA                                                                                        >  SRR4302537.4297483/1‑94 (MQ=60)

       AACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCA                                                                                        <  SRR4302537.5962733/94‑1 (MQ=60)

        ACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAA                                                                                       >  SRR4302537.6449403/1‑94 (MQ=60)

        ACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAA                                                                                       <  SRR4302537.6442503/94‑1 (MQ=60)

         CATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAAC                                                                                      <  SRR4302537.4010410/94‑1 (MQ=60)

          ATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACA                                                                                     <  SRR4302537.2041713/94‑1 (MQ=60)

            CCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAAT                                                                                   >  SRR4302537.1242303/1‑94 (MQ=60)

             CCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATC                                                                                  >  SRR4302537.362226/1‑94 (MQ=60)

             CCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATC                                                                                  <  SRR4302537.222736/94‑1 (MQ=60)

              CTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCT                                                                                 <  SRR4302537.2551639/94‑1 (MQ=60)

               TGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTC                                                                                >  SRR4302537.5057885/1‑94 (MQ=60)

                   GTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGC                                                                            >  SRR4302537.4515897/1‑94 (MQ=60)

                     AACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCAT                                                                          >  SRR4302537.2212989/1‑94 (MQ=60)

                     AACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCAT                                                                          >  SRR4302537.3313760/1‑94 (MQ=60)

                      ACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTCTCACGGAGTCAACAATCTCTTGCATT                                                                         >  SRR4302537.2451910/1‑94 (MQ=60)

                       CTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTT                                                                        <  SRR4302537.3593966/94‑1 (MQ=60)

                           CTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTTT                                                                    <  SRR4302537.3927080/94‑1 (MQ=60)

                             GTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGA                                                                  <  SRR4302537.4992981/94‑1 (MQ=60)

                              TTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGAT                                                                 <  SRR4302537.5818103/94‑1 (MQ=60)

                               TCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTTTGATT                                                                >  SRR4302537.6182607/1‑94 (MQ=60)

                                CGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTG                                                               <  SRR4302537.2832327/94‑1 (MQ=60)

                                 gaatggtctgatgAATAATTTTGGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAAAAATCTCTTGCATTTCTTTGATTGA                                                              <  SRR4302537.5192993/81‑1 (MQ=60)

                                 GAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTTTGATTGA                                                              >  SRR4302537.3092856/1‑94 (MQ=60)

                                 GAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGA                                                              <  SRR4302537.5192992/94‑1 (MQ=60)

                                 GAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGA                                                              <  SRR4302537.6514006/94‑1 (MQ=60)

                                   ATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATC                                                            >  SRR4302537.4790799/1‑94 (MQ=60)

                                    TTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCC                                                           <  SRR4302537.3203348/94‑1 (MQ=60)

                                    TTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCC                                                           <  SRR4302537.3111/94‑1 (MQ=60)

                                    TTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCC                                                           >  SRR4302537.2800958/1‑94 (MQ=60)

                                     TTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCC                                                          <  SRR4302537.10900/94‑1 (MQ=60)

                                        CTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACT                                                       <  SRR4302537.4119412/94‑1 (MQ=60)

                                        CTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACT                                                       >  SRR4302537.1813465/1‑94 (MQ=60)

                                        CTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACT                                                       <  SRR4302537.3441959/94‑1 (MQ=60)

                                        CTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACT                                                       >  SRR4302537.5335342/1‑94 (MQ=60)

                                           AGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCA                                                    >  SRR4302537.3066703/1‑94 (MQ=60)

                                            GAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCAT                                                   >  SRR4302537.4234844/1‑94 (MQ=60)

                                             AAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATA                                                  >  SRR4302537.1802185/1‑94 (MQ=60)

                                             AAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATA                                                  <  SRR4302537.2039338/94‑1 (MQ=60)

                                              AATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATAT                                                 <  SRR4302537.2532768/94‑1 (MQ=60)

                                               ATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATC                                                >  SRR4302537.4946595/1‑94 (MQ=60)

                                                TAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCC                                               >  SRR4302537.2836536/1‑94 (MQ=60)

                                                   TTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTAT                                            <  SRR4302537.440212/94‑1 (MQ=60)

                                                    TTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATT                                           <  SRR4302537.2884396/94‑1 (MQ=60)

                                                     TTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTT                                          >  SRR4302537.5155893/1‑94 (MQ=60)

                                                     TTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTC                                          >  SRR4302537.3920643/1‑94 (MQ=60)

                                                      TTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCT                                         <  SRR4302537.6316614/94‑1 (MQ=60)

                                                       TGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTA                                        >  SRR4302537.297645/1‑94 (MQ=60)

                                                       TGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTA                                        >  SRR4302537.3215389/1‑94 (MQ=60)

                                                        GTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTAT                                       >  SRR4302537.5398449/1‑94 (MQ=60)

                                                        GTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTAT                                       <  SRR4302537.2348971/94‑1 (MQ=60)

                                                        GTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTAT                                       <  SRR4302537.4598221/94‑1 (MQ=60)

                                                        GTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTAT                                       >  SRR4302537.5083972/1‑94 (MQ=60)

                                                         TATTCTCACTAATTTGCTTATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATT                                      <  SRR4302537.1513803/94‑1 (MQ=60)

                                                         TATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATT                                      <  SRR4302537.344478/94‑1 (MQ=60)

                                                          ATTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTA                                     >  SRR4302537.5629627/1‑94 (MQ=60)

                                                           TTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTAT                                    <  SRR4302537.2639217/94‑1 (MQ=60)

                                                           TTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTAT                                    >  SRR4302537.6489281/1‑94 (MQ=60)

                                                           TTCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTAT                                    <  SRR4302537.5546616/94‑1 (MQ=60)

                                                           TGCTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTAT                                    >  SRR4302537.6136573/1‑94 (MQ=60)

                                                             CTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCT                                  >  SRR4302537.5447729/1‑94 (MQ=60)

                                                             CTCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCT                                  <  SRR4302537.751497/94‑1 (MQ=60)

                                                              TCACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTA                                 >  SRR4302537.281319/1‑94 (MQ=60)

                                                               CACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTAC                                <  SRR4302537.1021890/94‑1 (MQ=60)

                                                               CACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTAC                                >  SRR4302537.2017548/1‑94 (MQ=60)

                                                                ACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACA                               <  SRR4302537.562373/94‑1 (MQ=60)

                                                                ACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACA                               >  SRR4302537.5130337/1‑94 (MQ=60)

                                                                ACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACA                               >  SRR4302537.4895427/1‑94 (MQ=60)

                                                                ACTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACA                               <  SRR4302537.2306287/94‑1 (MQ=60)

                                                                 CTAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAA                              >  SRR4302537.5588877/1‑94 (MQ=60)

                                                                  TAATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAAT                             >  SRR4302537.5504989/1‑94 (MQ=60)

                                                                   AATTTGCTTATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATG                            >  SRR4302537.6151332/1‑94 (MQ=60)

                                                                   AATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATG                            >  SRR4302537.3164032/1‑94 (MQ=60)

                                                                    ATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGA                           >  SRR4302537.4327931/1‑94 (MQ=60)

                                                                    ATTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGA                           >  SRR4302537.1610734/1‑94 (MQ=60)

                                                                     TTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAA                          >  SRR4302537.6248899/1‑94 (MQ=60)

                                                                     TTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAA                          >  SRR4302537.2390042/1‑94 (MQ=60)

                                                                     TTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAA                          <  SRR4302537.368623/94‑1 (MQ=60)

                                                                     TTTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGAATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAA                          <  SRR4302537.5522763/94‑1 (MQ=60)

                                                                      TTGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAA                         >  SRR4302537.2737677/1‑94 (MQ=60)

                                                                      TTGCTTATAAGTTTCTATTGTAACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAA                         <  SRR4302537.4194633/94‑1 (MQ=60)

                                                                       TGCTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAAT                        <  SRR4302537.4992498/94‑1 (MQ=60)

                                                                         CTTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCC                      >  SRR4302537.1859155/1‑94 (MQ=60)

                                                                          TTATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCG                     >  SRR4302537.4972298/1‑94 (MQ=60)

                                                                          TTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCG                     >  SRR4302537.4501067/1‑94 (MQ=60)

                                                                          TTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCG                     <  SRR4302537.4004297/94‑1 (MQ=60)

                                                                          TTATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCG                     <  SRR4302537.4004298/94‑1 (MQ=60)

                                                                           TATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGC                    >  SRR4302537.3219724/1‑94 (MQ=60)

                                                                           TATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTCATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGC                    <  SRR4302537.4588541/94‑1 (MQ=60)

                                                                            ATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCA                   <  SRR4302537.5580485/94‑1 (MQ=60)

                                                                            ATAAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCA                   >  SRR4302537.4246209/1‑94 (MQ=60)

                                                                              AAGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAG                 <  SRR4302537.4666174/94‑1 (MQ=60)

                                                                               AGTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGG                <  SRR4302537.6192528/94‑1 (MQ=60)

                                                                                GTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGG               <  SRR4302537.3262633/94‑1 (MQ=60)

                                                                                GTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGG               <  SRR4302537.3663021/94‑1 (MQ=60)

                                                                                GTTTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGG               >  SRR4302537.2592256/1‑94 (MQ=60)

                                                                                  TTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCG             <  SRR4302537.1801502/94‑1 (MQ=60)

                                                                                  TTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCG             >  SRR4302537.1804416/1‑94 (MQ=60)

                                                                                  TTCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCG             <  SRR4302537.2712714/94‑1 (MQ=60)

                                                                                   TCTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGG            <  SRR4302537.6088716/94‑1 (MQ=60)

                                                                                    CTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGC           >  SRR4302537.3362511/1‑94 (MQ=60)

                                                                                    CTATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGC           >  SRR4302537.5441997/1‑94 (MQ=60)

                                                                                     TATTGTCATGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCCGCT          >  SRR4302537.4323076/1‑94 (MQ=60)

                                                                                     TATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCT          >  SRR4302537.3420469/1‑94 (MQ=60)

                                                                                     TATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATACGCAAGGGCGGCT          >  SRR4302537.6366966/1‑94 (MQ=60)

                                                                                      ATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTT         <  SRR4302537.3585176/94‑1 (MQ=60)

                                                                                      ATTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTT         >  SRR4302537.4796299/1‑94 (MQ=60)

                                                                                       TTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTTT        <  SRR4302537.1761299/94‑1 (MQ=60)

                                                                                       TTGTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTTT        <  SRR4302537.1235914/94‑1 (MQ=60)

                                                                                         GTCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTTTAT      <  SRR4302537.698890/94‑1 (MQ=60)

                                                                                          TCACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTTTATC     >  SRR4302537.5896744/1‑94 (MQ=60)

                                                                                           CACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTTTATCC    >  SRR4302537.3630491/1‑94 (MQ=60)

                                                                                           CACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTTTATCC    >  SRR4302537.4446274/1‑94 (MQ=60)

                                                                                           CACGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTTTATCC    >  SRR4302537.783412/1‑94 (MQ=60)

                                                                                             CGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTTTATCCCA  >  SRR4302537.6217370/1‑94 (MQ=60)

CTTCAAGAACATCCCTGAAGTAACTTCCTGTTCGAATTTTCTGAGAAATAATTTTTGTATTCTCACTAATTTGCTTATAAGTTTCTATTGTCATGGAGTCAACAATCTCTTGCATTTCTTTGATTGATCCCACTGCATATCCTATTCTATTATCTACAATGAAATCCGCAAGGGCGGCTTTATCCCA  >  NC_000913/2103116‑2103302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`