Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,126,663	T→C	D83G (GAC→GGC)	wcaF ←	putative transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,126,663	0	T	C	95.7%	731.7 / 16.1	233	D83G (GAC→GGC)	wcaF	putative transferase
Reads supporting (aligned to +/- strand): ref base T (5/5); new base C (111/112); total (116/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 240 total aligned reads displayed.

TGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATT  >  NC_000913/2126573‑2126752

tGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCa                                                                                         <  1:6023357/93‑1 (MQ=255)

tGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCa                                                                                         <  2:1964003/93‑1 (MQ=255)

  gTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATc                                                                                       >  2:4159765/1‑93 (MQ=255)

  gTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATc                                                                                       <  1:559858/93‑1 (MQ=255)

      tGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCg                                                                                   >  2:2547851/1‑93 (MQ=255)

      tGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCg                                                                                   >  2:4355251/1‑93 (MQ=255)

       ggCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGa                                                                                  <  2:908102/93‑1 (MQ=255)

        gCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGAc                                                                                 <  2:4511725/93‑1 (MQ=255)

         cTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGAcc                                                                                >  1:1448675/1‑93 (MQ=255)

           aCCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCa                                                                              <  1:514054/93‑1 (MQ=255)

           aCCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCa                                                                              >  1:589705/1‑93 (MQ=255)

           aCCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCa                                                                              >  2:2216897/1‑93 (MQ=255)

           aCCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCa                                                                              >  2:4947685/1‑93 (MQ=255)

            ccGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCAc                                                                             >  1:2822006/1‑93 (MQ=255)

             cGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCAcg                                                                            <  1:5643238/93‑1 (MQ=255)

              ggTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCAcgc                                                                           <  2:6408163/93‑1 (MQ=255)

               gTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCAcgcg                                                                          >  1:741503/1‑93 (MQ=255)

                 gCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTa                                                                        >  1:963585/1‑93 (MQ=255)

                 gCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTa                                                                        <  2:1364368/93‑1 (MQ=255)

                  cATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTaa                                                                       <  1:4292212/93‑1 (MQ=255)

                   ataaataaCTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAAt                                                                      >  1:4926254/1‑93 (MQ=255)

                    taaataaCTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATc                                                                     <  1:3757585/93‑1 (MQ=255)

                     aaataaCTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCa                                                                    <  1:1334177/93‑1 (MQ=255)

                       ataaCTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCAcc                                                                  >  1:5396303/1‑93 (MQ=255)

                       ataaCTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCAcc                                                                  >  1:5618999/1‑93 (MQ=255)

                       ataaCTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCAcc                                                                  <  2:6265551/93‑1 (MQ=255)

                        taaCTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCt                                                                 >  1:1594743/1‑93 (MQ=255)

                        taaCTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCt                                                                 >  2:5575555/1‑93 (MQ=255)

                         aaCTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTa                                                                <  1:895549/93‑1 (MQ=255)

                         aaCTTTTTCGCGATATCGCCGAACGGGCGCCAATGGTTATTTCACCGAGGTTATATAAAGGGACGCCATCGCCGACCCACGCGTAATCACCTa                                                                <  2:3182806/93‑1 (MQ=255)

                          aCTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTaa                                                               >  2:3435940/1‑93 (MQ=255)

                           cTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAg                                                              <  2:4726322/93‑1 (MQ=255)

                           cTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAg                                                              >  2:1345217/1‑93 (MQ=255)

                           cTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAg                                                              <  2:3590675/93‑1 (MQ=255)

                             tttttGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGt                                                            <  1:5864874/93‑1 (MQ=255)

                             tttttGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGt                                                            <  1:2292204/93‑1 (MQ=255)

                                ttGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTaa                                                         >  1:5766172/1‑93 (MQ=255)

                                 tGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAAt                                                        >  2:1223001/1‑93 (MQ=255)

                                 tGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAAt                                                        >  2:1079928/1‑93 (MQ=255)

                                  gCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAAtt                                                       <  1:2649866/93‑1 (MQ=255)

                                  gCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAAtt                                                       <  2:2863366/93‑1 (MQ=255)

                                   cGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAAttt                                                      >  1:5514850/1‑93 (MQ=255)

                                       atCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCAc                                                  >  2:2282950/1‑93 (MQ=255)

                                          aCCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGa                                               >  1:4488328/1‑93 (MQ=255)

                                          aCCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGa                                               <  2:2473525/93‑1 (MQ=255)

                                          aCCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGa                                               <  1:3251926/93‑1 (MQ=255)

                                            cGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCAACGCGTAATCACCTAAGGTTAATTTCCACGGATa                                             >  2:5838794/1‑93 (MQ=255)

                                             gAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAAGTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAg                                            <  1:40739/93‑1 (MQ=255)

                                              aaTGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAgg                                           <  2:2792135/93‑1 (MQ=255)

                                              aaTGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAgg                                           <  1:2440396/93‑1 (MQ=255)

                                               aTGTGCGCTAATGGGTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATCGCCACGGATAGGt                                          <  2:1032200/93‑1 (MQ=255)

                                                tgtgCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTa                                         <  2:3041265/93‑1 (MQ=255)

                                                 gtgCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTaa                                        <  2:3935037/93‑1 (MQ=255)

                                                 gtgCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTaa                                        <  2:750674/93‑1 (MQ=255)

                                                     gcCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAAtttt                                    >  2:717234/1‑93 (MQ=255)

                                                      ccAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAAttttt                                   >  1:1102056/1‑93 (MQ=255)

                                                       cAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTa                                  >  1:254117/1‑93 (MQ=255)

                                                       cAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTa                                  <  1:1030290/93‑1 (MQ=255)

                                                       cAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTa                                  >  2:6097292/1‑93 (MQ=255)

                                                       cAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTa                                  >  1:510192/1‑93 (MQ=255)

                                                        aaTGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTat                                 >  2:3783232/1‑92 (MQ=255)

                                                        aaTGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTAc                                 <  1:3913852/93‑1 (MQ=255)

                                                        aaTGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTAc                                 >  2:5676054/1‑93 (MQ=255)

                                                         aTGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACt                                <  1:4912747/93‑1 (MQ=255)

                                                           ggTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTga                              >  2:2462576/1‑93 (MQ=255)

                                                           ggTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTga                              <  2:378919/93‑1 (MQ=255)

                                                            gTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTgac                             <  1:2086023/93‑1 (MQ=255)

                                                            gTTATTGCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTgac                             <  1:4586578/93‑1 (MQ=255)

                                                              tATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTgacgg                           >  1:2694644/1‑93 (MQ=255)

                                                               aTTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTgacgga                          <  2:6181724/93‑1 (MQ=255)

                                                               aTTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTgacgga                          >  1:2191640/1‑93 (MQ=255)

                                                               aTTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTgacgga                          <  1:2947665/93‑1 (MQ=255)

                                                                tttCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTgacggac                         >  1:4809580/1‑93 (MQ=255)

                                                                 ttCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTgacggacg                        <  2:1448287/93‑1 (MQ=255)

                                                                  tCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGa                       >  2:6461550/1‑93 (MQ=255)

                                                                   cagcGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGaa                      <  2:2730754/90‑1 (MQ=255)

                                                                   cACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGaa                      <  2:820353/93‑1 (MQ=255)

                                                                     ccGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATa                    >  2:5995264/1‑93 (MQ=255)

                                                                      cGAGCGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATaa                   <  2:5025738/93‑1 (MQ=255)

                                                                       gAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAAc                  <  1:666177/93‑1 (MQ=255)

                                                                       gAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAAc                  >  2:4211689/1‑93 (MQ=255)

                                                                         gggTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTa                <  2:3151335/93‑1 (MQ=255)

                                                                         gggTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTa                <  2:1173817/93‑1 (MQ=255)

                                                                          ggTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTAc               <  1:5570026/93‑1 (MQ=255)

                                                                           gTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACg              >  2:5125025/1‑93 (MQ=255)

                                                                             atataAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGtt            <  2:640074/93‑1 (MQ=255)

                                                                              tataAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGttt           <  2:3740623/93‑1 (MQ=255)

                                                                               ataAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGtttt          >  1:3426099/1‑93 (MQ=255)

                                                                               ataAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGtttt          <  1:851257/93‑1 (MQ=255)

                                                                                 aaaTTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGtttttt        <  2:5421207/93‑1 (MQ=255)

                                                                                 aaaTTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGtttttt        <  2:4862920/93‑1 (MQ=255)

                                                                                  aaTTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTc       <  1:3668869/93‑1 (MQ=255)

                                                                                   aTTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTcc      >  1:4215146/1‑93 (MQ=255)

                                                                                   aTTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTcc      <  1:720132/93‑1 (MQ=255)

                                                                                   aTTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTcc      >  1:6415465/1‑93 (MQ=255)

                                                                                    ttGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCt     >  2:1869828/1‑93 (MQ=255)

                                                                                     tGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTa    <  1:617922/93‑1 (MQ=255)

                                                                                      gACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTAt   >  1:2050731/1‑93 (MQ=255)

                                                                                      gACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTAt   >  1:5667624/1‑93 (MQ=255)

                                                                                       aCGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTAtt  >  2:3686216/1‑93 (MQ=255)

TGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATT  >  NC_000913/2126573‑2126752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTG  >  NC_000913/2126571‑2126756

CATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCC                                                                                              >  SRR4302537.190421/1‑94 (MQ=60)

 ATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCA                                                                                             >  SRR4302537.2476246/1‑94 (MQ=60)

 ATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCA                                                                                             <  SRR4302537.5496757/94‑1 (MQ=60)

  TGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCAT                                                                                            <  SRR4302537.6064740/94‑1 (MQ=60)

  TGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCAT                                                                                            <  SRR4302537.1589052/94‑1 (MQ=60)

   GGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATC                                                                                           <  SRR4302537.5017602/94‑1 (MQ=60)

   GGTCGT‑GCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGGATATAAATTGACCCCATCG                                                                                          >  SRR4302537.185622/1‑94 (MQ=60)

    GTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCG                                                                                          <  SRR4302537.563714/94‑1 (MQ=60)

    GTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCG                                                                                          <  SRR4302537.2772134/94‑1 (MQ=60)

    GTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCG                                                                                          <  SRR4302537.5649905/94‑1 (MQ=60)

      CGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCGCCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCC                                                                                        <  SRR4302537.668289/94‑1 (MQ=60)

      CGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCC                                                                                        <  SRR4302537.492972/94‑1 (MQ=60)

          GCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACC                                                                                    >  SRR4302537.1458768/1‑94 (MQ=60)

            TACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCA                                                                                  >  SRR4302537.593731/1‑94 (MQ=60)

             ACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCAC                                                                                 <  SRR4302537.517586/94‑1 (MQ=60)

             ACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCAC                                                                                 >  SRR4302537.2841733/1‑94 (MQ=60)

              CCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACG                                                                                <  SRR4302537.4515768/94‑1 (MQ=60)

              CCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACG                                                                                <  SRR4302537.878967/94‑1 (MQ=60)

               CGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGC                                                                               <  SRR4302537.5681857/94‑1 (MQ=60)

                GGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCG                                                                              <  SRR4302537.5722063/94‑1 (MQ=60)

                GGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCG                                                                              >  SRR4302537.746568/1‑94 (MQ=60)

                GGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCG                                                                              <  SRR4302537.5963423/94‑1 (MQ=60)

                GGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCG                                                                              >  SRR4302537.4061612/1‑94 (MQ=60)

                GGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCG                                                                              <  SRR4302537.5922205/94‑1 (MQ=60)

                  TGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTA                                                                            <  SRR4302537.5469025/94‑1 (MQ=60)

                  TGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTA                                                                            >  SRR4302537.144275/1‑94 (MQ=60)

                  TGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTA                                                                            >  SRR4302537.970234/1‑94 (MQ=60)

                    CATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAAT                                                                          >  SRR4302537.4960213/1‑94 (MQ=60)

                    CATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAAT                                                                          <  SRR4302537.4322044/94‑1 (MQ=60)

                     ATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATC                                                                         >  SRR4302537.5680830/1‑94 (MQ=60)

                      TAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCA                                                                        >  SRR4302537.2251491/1‑94 (MQ=60)

                      TAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCA                                                                        >  SRR4302537.6487407/1‑94 (MQ=60)

                      TAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCA                                                                        <  SRR4302537.3783963/94‑1 (MQ=60)

                      TAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCA                                                                        >  SRR4302537.5553298/1‑94 (MQ=60)

                       AAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCAC                                                                       <  SRR4302537.561092/94‑1 (MQ=60)

                       AAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCAC                                                                       <  SRR4302537.1343467/94‑1 (MQ=60)

                        AATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACC                                                                      >  SRR4302537.5433149/1‑94 (MQ=60)

                        AATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACC                                                                      >  SRR4302537.1454597/1‑94 (MQ=60)

                        AATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACC                                                                      >  SRR4302537.5657423/1‑94 (MQ=60)

                         ATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCT                                                                     >  SRR4302537.1605887/1‑94 (MQ=60)

                          TAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTA                                                                    >  SRR4302537.3781135/1‑94 (MQ=60)

                           AACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAA                                                                   <  SRR4302537.901732/94‑1 (MQ=60)

                             CTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGG                                                                 <  SRR4302537.3443913/94‑1 (MQ=60)

                              TTTTTTGCGATATCACCGAATGTGCGCGAATGGTTATCTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGT                                                                <  SRR4302537.421469/94‑1 (MQ=60)

                               TTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTT                                                               >  SRR4302537.2171651/1‑94 (MQ=60)

                               TTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTT                                                               <  SRR4302537.2308386/94‑1 (MQ=60)

                               TTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTT                                                               <  SRR4302537.5905100/94‑1 (MQ=60)

                                 TTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAA                                                             >  SRR4302537.5805668/1‑94 (MQ=60)

                                   TGCGATATCACCGAATGTGCGCCAATGGTTATTACACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATT                                                           <  SRR4302537.999278/94‑1 (MQ=60)

                                    GCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTT                                                          <  SRR4302537.2668399/94‑1 (MQ=60)

                                    GCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTT                                                          >  SRR4302537.5552506/1‑94 (MQ=60)

                                       ATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCA                                                       >  SRR4302537.2708788/1‑94 (MQ=60)

                                         ATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACG                                                     >  SRR4302537.5810725/1‑94 (MQ=60)

                                          TCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGG                                                    >  SRR4302537.3807519/1‑94 (MQ=60)

                                          TCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGG                                                    >  SRR4302537.3710044/1‑94 (MQ=60)

                                           CACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGA                                                   >  SRR4302537.3663661/1‑94 (MQ=60)

                                           CACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGA                                                   >  SRR4302537.4519377/1‑94 (MQ=60)

                                            ACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGAT                                                  <  SRR4302537.3274849/94‑1 (MQ=60)

                                             CCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATA                                                 >  SRR4302537.2082995/1‑94 (MQ=60)

                                             CCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATA                                                 >  SRR4302537.950662/1‑94 (MQ=60)

                                              CGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAG                                                >  SRR4302537.91534/1‑94 (MQ=60)

                                               GAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGG                                               >  SRR4302537.5411752/1‑94 (MQ=60)

                                               GAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAAGTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGG                                               <  SRR4302537.41045/94‑1 (MQ=60)

                                                AATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGT                                              <  SRR4302537.4502250/94‑1 (MQ=60)

                                                AATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGT                                              <  SRR4302537.2457580/94‑1 (MQ=60)

                                                AATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGT                                              <  SRR4302537.4831803/94‑1 (MQ=60)

                                                  TGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAA                                            <  SRR4302537.2288337/94‑1 (MQ=60)

                                                      CGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTT                                        >  SRR4302537.5673085/1‑94 (MQ=60)

                                                       GCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTT                                       >  SRR4302537.1040703/1‑94 (MQ=60)

                                                       GCCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTT                                       >  SRR4302537.1109671/1‑94 (MQ=60)

                                                        CCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTA                                      <  SRR4302537.1127057/94‑1 (MQ=60)

                                                        CCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTA                                      >  SRR4302537.513693/1‑94 (MQ=60)

                                                        CCAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTA                                      >  SRR4302537.255920/1‑94 (MQ=60)

                                                         CAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTAC                                     <  SRR4302537.1037386/94‑1 (MQ=60)

                                                         CAATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTAC                                     <  SRR4302537.696809/94‑1 (MQ=60)

                                                          AATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACT                                    <  SRR4302537.3941212/94‑1 (MQ=60)

                                                           ATGGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTG                                   <  SRR4302537.4946652/94‑1 (MQ=60)

                                                             GGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGAC                                 >  SRR4302537.740251/1‑94 (MQ=60)

                                                             GGTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGAC                                 <  SRR4302537.570439/94‑1 (MQ=60)

                                                              GTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACG                                <  SRR4302537.2100731/94‑1 (MQ=60)

                                                              GTTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACG                                <  SRR4302537.6302913/94‑1 (MQ=60)

                                                              GTTATTGCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACG                                <  SRR4302537.4618303/94‑1 (MQ=60)

                                                               TTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGG                               <  SRR4302537.4949004/94‑1 (MQ=60)

                                                               TTATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGG                               >  SRR4302537.2713474/1‑94 (MQ=60)

                                                                TATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGA                              >  SRR4302537.4002099/1‑94 (MQ=60)

                                                                TATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGA                              >  SRR4302537.2207081/1‑94 (MQ=60)

                                                                 ATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGAC                             >  SRR4302537.4842779/1‑94 (MQ=60)

                                                                 ATTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGAC                             <  SRR4302537.2968355/94‑1 (MQ=60)

                                                                  TTTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACG                            <  SRR4302537.2965701/94‑1 (MQ=60)

                                                                   TTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGA                           >  SRR4302537.598466/1‑94 (MQ=60)

                                                                   TTCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGA                           <  SRR4302537.4812277/94‑1 (MQ=60)

                                                                   TTCACCGAGGGTATATAAATTGAAGCCATAGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTCACGGACGA                           >  SRR4302537.829842/1‑94 (MQ=60)

                                                                    TCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAA                          >  SRR4302537.4610051/1‑94 (MQ=60)

                                                                    TCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAA                          >  SRR4302537.3150801/1‑94 (MQ=60)

                                                                    TCACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGAAGAA                          <  SRR4302537.4303145/94‑1 (MQ=60)

                                                                      ACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATA                        <  SRR4302537.4986888/94‑1 (MQ=60)

                                                                      ACCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATA                        >  SRR4302537.2512596/1‑94 (MQ=60)

                                                                       CCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAA                       <  SRR4302537.5139707/94‑1 (MQ=60)

                                                                       CCGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAA                       >  SRR4302537.2452712/1‑94 (MQ=60)

                                                                        CGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAAC                      <  SRR4302537.5883829/94‑1 (MQ=60)

                                                                        CGAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAAC                      >  SRR4302537.2650775/1‑94 (MQ=60)

                                                                         GAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACT                     <  SRR4302537.670717/94‑1 (MQ=60)

                                                                         GAGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACT                     <  SRR4302537.5619260/94‑1 (MQ=60)

                                                                          AGGGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTA                    >  SRR4302537.6084020/1‑94 (MQ=60)

                                                                            GGTATATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACG                  <  SRR4302537.5608100/94‑1 (MQ=60)

                                                                                TATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTT              >  SRR4302537.319266/1‑94 (MQ=60)

                                                                                TATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTT              >  SRR4302537.3450350/1‑94 (MQ=60)

                                                                                 ATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTT             <  SRR4302537.857092/94‑1 (MQ=60)

                                                                                 ATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTT             <  SRR4302537.1634874/94‑1 (MQ=60)

                                                                                 ATAAATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACCAATAAATACGGTTTT             >  SRR4302537.730492/1‑94 (MQ=60)

                                                                                    AATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCC          <  SRR4302537.3694684/94‑1 (MQ=60)

                                                                                    AATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCC          >  SRR4302537.4244533/1‑94 (MQ=60)

                                                                                    AATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCC          >  SRR4302537.6459099/1‑94 (MQ=60)

                                                                                     ATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCT         <  SRR4302537.725050/94‑1 (MQ=60)

                                                                                     ATTGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCT         <  SRR4302537.2781393/94‑1 (MQ=60)

                                                                                       TGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTAT       <  SRR4302537.622126/94‑1 (MQ=60)

                                                                                       TGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTAT       >  SRR4302537.2065115/1‑94 (MQ=60)

                                                                                       TGACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTAT       >  SRR4302537.5706405/1‑94 (MQ=60)

                                                                                        GACGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATT      <  SRR4302537.5212451/94‑1 (MQ=60)

                                                                                          CGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTT    >  SRR4302537.2147087/1‑94 (MQ=60)

                                                                                          CGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTT    >  SRR4302537.6257969/1‑94 (MQ=60)

                                                                                          CGCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTT    <  SRR4302537.2858949/94‑1 (MQ=60)

                                                                                           GCCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTT   >  SRR4302537.2199070/1‑94 (MQ=60)

                                                                                            CCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTG  <  SRR4302537.5682704/94‑1 (MQ=60)

                                                                                            CCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTG  >  SRR4302537.1745930/1‑94 (MQ=60)

                                                                                            CCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTG  <  SRR4302537.3069/94‑1 (MQ=60)

CATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTCCACGGATAGGTAATTTTTACTGACGGACGAATAACTACGTTTTTTCCTATTTTTG  >  NC_000913/2126571‑2126756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`