Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,183,364 T→C intergenic (‑43/+180) thiM ← / ← yohL hydoxyethylthiazole kinase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,183,3640TC96.3% 701.1 / 11.8 218intergenic (‑43/+180)thiM/yohLhydoxyethylthiazole kinase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (4/4);  new base C (107/103);  total (111/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01

Only 100 of 231 total aligned reads displayed.

GTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTG  >  NC_000913/2183274‑2183455
                                                                                          |                                                                                           
gtgtAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATACCGTGAAGAAGCAATGACCTGGTGGccc                                                                                           <  1:3193772/93‑1 (MQ=255)
gtgtAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGccc                                                                                           >  1:2291835/1‑93 (MQ=255)
   tAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTg                                                                                        >  2:1001640/1‑93 (MQ=255)
     aCGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGAc                                                                                      <  2:1550004/93‑1 (MQ=255)
      cgcgTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACt                                                                                     >  1:2315561/1‑93 (MQ=255)
          tGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTccc                                                                                 <  2:1966173/93‑1 (MQ=255)
          tGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTccc                                                                                 <  2:3352326/93‑1 (MQ=255)
           gcgcAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCt                                                                                <  2:922273/93‑1 (MQ=255)
             gcAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTAc                                                                              >  1:3631274/1‑93 (MQ=255)
             gcAGATTGCGCGCAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTAc                                                                              <  1:2073799/93‑1 (MQ=255)
                gATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCt                                                                           >  2:3727644/1‑93 (MQ=255)
                 aTTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTg                                                                          <  2:4885395/93‑1 (MQ=255)
                 aTTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTg                                                                          >  1:4296549/1‑93 (MQ=255)
                      gcTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCAtt                                                                     >  1:162447/1‑93 (MQ=255)
                      gcTGAACCCAGCAGGGCGACTTCCATAGTTTTCTTCTGCCTTAACGTGAAGAAGCAATGACCTGGTGGCCCGGGACTTCCCTCCCCTGGCAtt                                                                     >  2:4427732/1‑93 (MQ=255)
                       cTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAATAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGATGGCATTa                                                                    >  2:6381376/1‑93 (MQ=255)
                       cTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTa                                                                    >  1:2831060/1‑93 (MQ=255)
                       cTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTa                                                                    >  1:4041293/1‑93 (MQ=255)
                         gAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATc                                                                  >  1:1273267/1‑93 (MQ=255)
                         gAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATc                                                                  >  1:893303/1‑93 (MQ=255)
                           aCCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCa                                                                <  1:5275713/93‑1 (MQ=255)
                           aCCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCGGGGGGCCGGTGACTTCCCTCCGCTGGCTTTATCCa                                                                >  1:5759837/1‑93 (MQ=255)
                            cccAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAg                                                               <  1:4487934/93‑1 (MQ=255)
                            cccAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAg                                                               >  1:4405805/1‑93 (MQ=255)
                             ccAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGa                                                              <  1:2636055/93‑1 (MQ=255)
                               agcagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATc                                                            >  2:5339922/1‑93 (MQ=255)
                               agcagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATc                                                            >  1:2422787/1‑93 (MQ=255)
                                gcagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCa                                                           <  2:5459847/93‑1 (MQ=255)
                                gcagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCa                                                           <  2:1084436/93‑1 (MQ=255)
                                gcagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCa                                                           >  2:414942/1‑93 (MQ=255)
                                 cagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAg                                                          <  1:1232068/93‑1 (MQ=255)
                                 cagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAg                                                          <  1:4849082/93‑1 (MQ=255)
                                  agGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAgg                                                         >  2:829867/1‑93 (MQ=255)
                                    gTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTg                                                       >  2:5731149/1‑93 (MQ=255)
                                     tCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGa                                                      <  1:2744238/93‑1 (MQ=255)
                                     tCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGa                                                      >  1:3932962/1‑93 (MQ=255)
                                     tCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGa                                                      >  2:5657491/1‑93 (MQ=255)
                                       gACTTGCATAGTTTGCTCCTGCCATAACCTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGATGGCATTATCCAGATCAGGTGAtc                                                    >  1:3123964/1‑92 (MQ=255)
                                        aCTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATAc                                                   >  1:5560287/1‑93 (MQ=255)
                                        aCTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATAc                                                   <  1:5108336/93‑1 (MQ=255)
                                        aCTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATAc                                                   <  1:3654787/93‑1 (MQ=255)
                                         cTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACg                                                  <  2:4229470/93‑1 (MQ=255)
                                         cTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACg                                                  >  1:6137014/1‑93 (MQ=255)
                                          ttGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACgg                                                 >  1:935335/1‑93 (MQ=255)
                                              aTAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTAt                                             >  2:3037478/1‑93 (MQ=255)
                                              aTAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTAt                                             <  2:2393207/93‑1 (MQ=255)
                                              aTAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTAt                                             <  2:2313063/93‑1 (MQ=255)
                                              aTAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTAt                                             >  1:3001766/1‑93 (MQ=255)
                                               tAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTAtt                                            >  2:1273461/1‑93 (MQ=255)
                                                aGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTAttt                                           >  1:1845508/1‑93 (MQ=255)
                                                 gTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTtc                                          >  2:2067864/1‑93 (MQ=255)
                                                 atttGCTCCTGCCATAACGTGAAGAAGAAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTtc                                          <  2:4688130/92‑1 (MQ=255)
                                                  tttGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTtct                                         <  1:6085730/93‑1 (MQ=255)
                                                   ttGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTtctc                                        <  1:6251813/93‑1 (MQ=255)
                                                   ttGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTtctc                                        >  1:2164171/1‑93 (MQ=255)
                                                    tGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCa                                       >  1:5402484/1‑93 (MQ=255)
                                                    tGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCa                                       <  1:5623770/93‑1 (MQ=255)
                                                     gCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAg                                      >  2:3503543/1‑93 (MQ=255)
                                                       tCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGcc                                    >  2:849492/1‑93 (MQ=255)
                                                        ccTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCt                                   <  1:1390568/93‑1 (MQ=255)
                                                         cTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCtt                                  <  2:6185082/93‑1 (MQ=255)
                                                         cTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCtt                                  >  2:2200988/1‑93 (MQ=255)
                                                           gCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCa                                <  2:4378348/93‑1 (MQ=255)
                                                            gcATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCAc                               <  1:3345586/92‑1 (MQ=255)
                                                             cATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACg                              >  2:1301420/1‑93 (MQ=255)
                                                              aTAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGc                             <  1:4459327/93‑1 (MQ=255)
                                                              aTAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGc                             <  1:4770364/93‑1 (MQ=255)
                                                               tAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCa                            <  2:5044274/93‑1 (MQ=255)
                                                               tAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCa                            >  2:2746715/1‑93 (MQ=255)
                                                               tAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCa                            <  2:2267711/93‑1 (MQ=255)
                                                                   gTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAg                        <  1:5946378/93‑1 (MQ=255)
                                                                    tGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAgg                       >  2:708596/1‑93 (MQ=255)
                                                                    tGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAgg                       >  1:3909689/1‑93 (MQ=255)
                                                                      aagaagCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGc                     >  1:4269935/1‑93 (MQ=255)
                                                                      aagaagCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGc                     >  1:4791611/1‑93 (MQ=255)
                                                                      aagaagCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGc                     >  2:3195162/1‑93 (MQ=255)
                                                                       agaagCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCa                    >  2:3631087/1‑93 (MQ=255)
                                                                        gaagCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCAc                   <  2:3572628/93‑1 (MQ=255)
                                                                         aagCAATTACCTTGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCATGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCAcc                  <  1:2148238/93‑1 (MQ=255)
                                                                         aagCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCAcc                  >  2:365490/1‑93 (MQ=255)
                                                                          agCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCAccc                 >  1:3871671/1‑93 (MQ=255)
                                                                           gCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCAcccc                >  2:2789662/1‑93 (MQ=255)
                                                                            cAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCg               >  2:3852184/1‑93 (MQ=255)
                                                                             aaTGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGa              >  2:5762713/1‑93 (MQ=255)
                                                                             aaTGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGa              <  2:5611598/93‑1 (MQ=255)
                                                                             aaTGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGa              >  1:195844/1‑93 (MQ=255)
                                                                              aTGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAg             >  2:2773288/1‑93 (MQ=255)
                                                                              aTGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAg             <  1:6369168/93‑1 (MQ=255)
                                                                              aTGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAg             >  1:2544624/1‑93 (MQ=255)
                                                                               tGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGt            <  2:3462599/93‑1 (MQ=255)
                                                                                gACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTc           <  2:5890353/93‑1 (MQ=255)
                                                                                 aCCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCg          <  1:1453036/93‑1 (MQ=255)
                                                                                  ccTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGt         >  2:1422791/1‑93 (MQ=255)
                                                                                   cTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGtt        >  1:3787625/1‑93 (MQ=255)
                                                                                   cTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGtt        <  2:1816394/93‑1 (MQ=255)
                                                                                    tggtggCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGttt       <  2:5991099/93‑1 (MQ=255)
                                                                                    tggtggCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGttt       <  2:5850023/93‑1 (MQ=255)
                                                                                     ggtggcCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTg      <  2:596097/93‑1 (MQ=255)
                                                                                     ggtggcCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGCATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTg      >  2:4980355/1‑93 (MQ=255)
                                                                                      gtggcCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTgg     >  2:2625665/1‑93 (MQ=255)
                                                                                          |                                                                                           
GTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTG  >  NC_000913/2183274‑2183455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTG  >  NC_000913/2183271‑2183455
                                                                                             |                                                                                           
AAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGC                                                                                             >  SRR4302537.2328995/1‑94 (MQ=60)
 AGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCC                                                                                            >  SRR4302537.553895/1‑94 (MQ=60)
 AGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCC                                                                                            >  SRR4302537.6454271/1‑94 (MQ=60)
  GGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCC                                                                                           <  SRR4302537.984985/94‑1 (MQ=60)
  GGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCC                                                                                           >  SRR4302537.2308016/1‑94 (MQ=60)
   GTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATACCGTGAAGAAGCAATGACCTGGTGGCCCG                                                                                          <  SRR4302537.3216230/94‑1 (MQ=60)
      TAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGA                                                                                       >  SRR4302537.5695549/1‑94 (MQ=60)
        ACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACT                                                                                     >  SRR4302537.2331907/1‑94 (MQ=60)
            GTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCC                                                                                 >  SRR4302537.5090180/1‑94 (MQ=60)
            GTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCC                                                                                 >  SRR4302537.230573/1‑94 (MQ=60)
               CGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTAC                                                                              <  SRR4302537.935273/94‑1 (MQ=60)
               CGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTAC                                                                              >  SRR4302537.3656859/1‑94 (MQ=60)
                GCAGATTGCGCGCAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACG                                                                             <  SRR4302537.2088404/94‑1 (MQ=60)
                  AGATTGCGCTGACCCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCT                                                                           <  SRR4302537.3782381/94‑1 (MQ=60)
                  AGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCT                                                                           <  SRR4302537.3196133/94‑1 (MQ=60)
                  AGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCT                                                                           >  SRR4302537.2233042/1‑94 (MQ=60)
                   GATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGNAGAAGCAANGACCTGGNGGCCCGTGACTTCNCNANGNTG                                                                          >  SRR4302537.1973545/1‑94 (MQ=60)
                   GATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTG                                                                          >  SRR4302537.1755324/1‑94 (MQ=60)
                   GATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTG                                                                          >  SRR4302537.4326415/1‑94 (MQ=60)
                        CGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATT                                                                     >  SRR4302537.5567174/1‑94 (MQ=60)
                        CGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATT                                                                     >  SRR4302537.163603/1‑94 (MQ=60)
                         GCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTA                                                                    >  SRR4302537.2850843/1‑94 (MQ=60)
                         GCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTA                                                                    >  SRR4302537.4069562/1‑94 (MQ=60)
                          CTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTAT                                                                   >  SRR4302537.6157612/1‑94 (MQ=60)
                           TGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATC                                                                  >  SRR4302537.1282118/1‑94 (MQ=60)
                           TGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATC                                                                  >  SRR4302537.899467/1‑94 (MQ=60)
                            GAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCC                                                                 <  SRR4302537.4033277/94‑1 (MQ=60)
                             AACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCA                                                                >  SRR4302537.2132763/1‑94 (MQ=60)
                             AACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCGGGGGGCCGGTGACTTCCCTCCGCTGGCTTTATCCA                                                                >  SRR4302537.5799289/1‑94 (MQ=60)
                              ACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAG                                                               >  SRR4302537.4436206/1‑94 (MQ=60)
                              ACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAG                                                               <  SRR4302537.5312017/94‑1 (MQ=60)
                               CCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGA                                                              <  SRR4302537.4518980/94‑1 (MQ=60)
                                CCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGAT                                                             >  SRR4302537.5960630/1‑94 (MQ=60)
                                CCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGAT                                                             <  SRR4302537.2654501/94‑1 (MQ=60)
                                CCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGAT                                                             <  SRR4302537.2471454/94‑1 (MQ=60)
                                 CAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATC                                                            >  SRR4302537.2439854/1‑94 (MQ=60)
                                 CAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATC                                                            <  SRR4302537.4496291/94‑1 (MQ=60)
                                   GCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAG                                                          <  SRR4302537.21660/94‑1 (MQ=60)
                                    CAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGG                                                         <  SRR4302537.2963079/94‑1 (MQ=60)
                                    CAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGG                                                         <  SRR4302537.4882542/94‑1 (MQ=60)
                                    CAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGG                                                         <  SRR4302537.1240638/94‑1 (MQ=60)
                                     AGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGT                                                        <  SRR4302537.3380320/94‑1 (MQ=60)
                                      GGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTG                                                       <  SRR4302537.2007303/94‑1 (MQ=60)
                                       GTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGA                                                      >  SRR4302537.276758/1‑94 (MQ=60)
                                       GTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGA                                                      >  SRR4302537.3960447/1‑94 (MQ=60)
                                       GTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGA                                                      >  SRR4302537.3960263/1‑94 (MQ=60)
                                        TCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGAT                                                     >  SRR4302537.4684102/1‑94 (MQ=60)
                                        TCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGAT                                                     <  SRR4302537.5041012/94‑1 (MQ=60)
                                        TCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGAT                                                     <  SRR4302537.3347017/94‑1 (MQ=60)
                                        TCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGAT                                                     <  SRR4302537.2763382/94‑1 (MQ=60)
                                         CGACTTGCATAGTTTGCTCCTGCCATAACCTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGATGGCATTATCCAGATCAGGTGATC                                                    >  SRR4302537.3145829/1‑94 (MQ=60)
                                          GACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATAC                                                   >  SRR4302537.5598281/1‑94 (MQ=60)
                                          GACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATAC                                                   <  SRR4302537.369499/94‑1 (MQ=60)
                                           ACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACG                                                  <  SRR4302537.6373033/94‑1 (MQ=60)
                                           ACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACG                                                  <  SRR4302537.6070266/94‑1 (MQ=60)
                                           ACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACG                                                  <  SRR4302537.5143537/94‑1 (MQ=60)
                                           ACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACG                                                  >  SRR4302537.6179208/1‑94 (MQ=60)
                                           ACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACG                                                  <  SRR4302537.3680507/94‑1 (MQ=60)
                                            CTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTCCGCTGGCATTATCCAGATCAGGTGATACGG                                                 >  SRR4302537.5610546/1‑94 (MQ=60)
                                            CTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGG                                                 >  SRR4302537.941781/1‑94 (MQ=60)
                                            CTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGG                                                 <  SRR4302537.4426548/94‑1 (MQ=60)
                                            CTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGG                                                 >  SRR4302537.435271/1‑94 (MQ=60)
                                                CATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTAT                                             >  SRR4302537.3022684/1‑94 (MQ=60)
                                                CATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTAT                                             >  SRR4302537.6216757/1‑94 (MQ=60)
                                                  TAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTT                                           <  SRR4302537.782906/94‑1 (MQ=60)
                                                  TAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTT                                           >  SRR4302537.1858350/1‑94 (MQ=60)
                                                  TAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTT                                           <  SRR4302537.4315892/94‑1 (MQ=60)
                                                   AGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTC                                          >  SRR4302537.3131237/1‑94 (MQ=60)
                                                   AGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTC                                          >  SRR4302537.2678137/1‑94 (MQ=60)
                                                     TTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTC                                        >  SRR4302537.2179417/1‑94 (MQ=60)
                                                     TTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTC                                        <  SRR4302537.6127538/94‑1 (MQ=60)
                                                      TTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCA                                       >  SRR4302537.5439351/1‑94 (MQ=60)
                                                      TTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCA                                       <  SRR4302537.6294762/94‑1 (MQ=60)
                                                      TTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCA                                       <  SRR4302537.1486382/94‑1 (MQ=60)
                                                       TGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAG                                      <  SRR4302537.5662229/94‑1 (MQ=60)
                                                       TGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAG                                      <  SRR4302537.2416066/94‑1 (MQ=60)
                                                         CTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCC                                    >  SRR4302537.4863138/1‑94 (MQ=60)
                                                          TCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCT                                   <  SRR4302537.6367814/94‑1 (MQ=60)
                                                           CCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTT                                  <  SRR4302537.1400266/94‑1 (MQ=60)
                                                           CCTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTT                                  >  SRR4302537.3254932/1‑94 (MQ=60)
                                                            CTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTC                                 >  SRR4302537.4164732/1‑94 (MQ=60)
                                                            CTGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTC                                 >  SRR4302537.3883155/1‑94 (MQ=60)
                                                             TGCCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCA                                <  SRR4302537.395295/94‑1 (MQ=60)
                                                              GCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGATATTTCTCAGCCTTCAC                               >  SRR4302537.1254690/1‑94 (MQ=60)
                                                               GCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACG                              <  SRR4302537.3369269/94‑1 (MQ=60)
                                                               CCATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACG                              <  SRR4302537.1991406/94‑1 (MQ=60)
                                                                CATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGC                             <  SRR4302537.5361860/94‑1 (MQ=60)
                                                                 ATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCA                            <  SRR4302537.4803302/94‑1 (MQ=60)
                                                                 ATAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCA                            <  SRR4302537.4490159/94‑1 (MQ=60)
                                                                  TAACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAG                           <  SRR4302537.998910/94‑1 (MQ=60)
                                                                   AACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGA                          <  SRR4302537.132719/94‑1 (MQ=60)
                                                                   AACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGA                          >  SRR4302537.4814885/1‑94 (MQ=60)
                                                                   AACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGA                          <  SRR4302537.1515328/94‑1 (MQ=60)
                                                                    ACGTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAA                         <  SRR4302537.1426996/94‑1 (MQ=60)
                                                                      GTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGG                       <  SRR4302537.5987192/94‑1 (MQ=60)
                                                                      GTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGG                       >  SRR4302537.3937018/1‑94 (MQ=60)
                                                                      GTGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGG                       >  SRR4302537.3905098/1‑94 (MQ=60)
                                                                       TGAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGGATTTCTCAGCCTTCACGCAGAAGGG                      >  SRR4302537.5851985/1‑94 (MQ=60)
                                                                        GAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGC                     >  SRR4302537.4824683/1‑94 (MQ=60)
                                                                        GAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGC                     <  SRR4302537.2318264/94‑1 (MQ=60)
                                                                        GAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGC                     >  SRR4302537.4299602/1‑94 (MQ=60)
                                                                        GAAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGC                     <  SRR4302537.5555122/94‑1 (MQ=60)
                                                                         AAGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCA                    <  SRR4302537.2635246/94‑1 (MQ=60)
                                                                          AGAAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCAC                   >  SRR4302537.5232555/1‑94 (MQ=60)
                                                                            AAGCAATTACCTTGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCATGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCC                 <  SRR4302537.2163382/94‑1 (MQ=60)
                                                                            AAGCAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCC                 >  SRR4302537.3898762/1‑94 (MQ=60)
                                                                               CAATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGA              >  SRR4302537.197237/1‑94 (MQ=60)
                                                                                AATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAG             >  SRR4302537.2562458/1‑94 (MQ=60)
                                                                                 ATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGT            >  SRR4302537.4524501/1‑94 (MQ=60)
                                                                                 ATGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGT            <  SRR4302537.6412609/94‑1 (MQ=60)
                                                                                  TGACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTC           >  SRR4302537.2994554/1‑94 (MQ=60)
                                                                                   GACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCG          <  SRR4302537.98459/94‑1 (MQ=60)
                                                                                    ACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGT         <  SRR4302537.1463165/94‑1 (MQ=60)
                                                                                    ACCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGGCGT         >  SRR4302537.1867364/1‑94 (MQ=60)
                                                                                     CCTGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTT        >  SRR4302537.3814219/1‑94 (MQ=60)
                                                                                       TGGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTG      >  SRR4302537.3666323/1‑94 (MQ=60)
                                                                                        GGTGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGG     >  SRR4302537.2531163/1‑94 (MQ=60)
                                                                                          TGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTT   >  SRR4302537.4545191/1‑94 (MQ=60)
                                                                                          TGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTT   <  SRR4302537.443644/94‑1 (MQ=60)
                                                                                          TGGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTT   >  SRR4302537.5756629/1‑94 (MQ=60)
                                                                                           GGCCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTG  <  SRR4302537.3055633/94‑1 (MQ=60)
                                                                                             |                                                                                           
AAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTG  >  NC_000913/2183271‑2183455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑