Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,252,644	C→G	G188A (GGC→GCC)	yeiK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,644	0	C	G	91.6%	564.5 / 36.0	180	G188A (GGC→GCC)	yeiK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (8/7); new base G (84/80); total (92/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-01

Only 100 of 188 total aligned reads displayed.

TCGCTGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAG  >  NC_000913/2252554‑2252733

  gCTGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGTGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCcat                                                                                       >  1:4556020/1‑93 (MQ=255)

  gCTGAACAGCTCTCCGGCGCGGCCGCCTGCCCTTTTCCTCCGAGCAATCACGTCCGGGGCGGCATCGGTCTGGTTGGAGAGATGGAGTCTcat                                                                                       >  2:520726/1‑93 (MQ=255)

   cTGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCcatc                                                                                      <  2:1165398/93‑1 (MQ=255)

    tGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCcatca                                                                                     >  2:1444021/1‑93 (MQ=255)

     gAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCcatcat                                                                                    >  1:642584/1‑93 (MQ=255)

      aaCAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCCAACGGTCTGGTTGGTGAGCTCGAGGGGCatcatc                                                                                   >  2:1602582/1‑93 (MQ=255)

      aaCAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCatcatc                                                                                   <  2:5016724/93‑1 (MQ=255)

        cAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCAc                                                                                 <  1:4093786/93‑1 (MQ=255)

         aGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACt                                                                                <  2:1248579/93‑1 (MQ=255)

          gCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTa                                                                               >  1:3232474/1‑93 (MQ=255)

          gCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTa                                                                               >  2:4702919/1‑93 (MQ=255)

            tctcCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGTTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAAt                                                                             <  2:3465531/93‑1 (MQ=255)

            tctcCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAAt                                                                             <  2:2374678/93‑1 (MQ=255)

                cggcggGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTTAGATCGAGGGCCATCATCACTAATGGaa                                                                         <  1:5162976/93‑1 (MQ=255)

                cggcggGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGaa                                                                         <  2:2959216/93‑1 (MQ=255)

                 ggcggGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAAc                                                                        <  2:6121332/93‑1 (MQ=255)

                  gcggGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACg                                                                       >  1:5722201/1‑93 (MQ=255)

                   cggGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGc                                                                      >  2:2751256/1‑93 (MQ=255)

                    ggggCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGcc                                                                     <  1:3182105/93‑1 (MQ=255)

                     gggCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCg                                                                    >  1:3724122/1‑93 (MQ=255)

                       gccgccTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGa                                                                  <  1:4674033/93‑1 (MQ=255)

                        ccgccTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAg                                                                 <  2:5149167/93‑1 (MQ=255)

                          gccTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGt                                                               <  1:1024027/93‑1 (MQ=255)

                          gccTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGt                                                               >  1:2004366/1‑93 (MQ=255)

                           ccTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTg                                                              >  1:4902141/1‑93 (MQ=255)

                           ccTGCCCTTTCCATCCGAGCAATCACGTCCGGGGGGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTg                                                              >  2:5442350/1‑93 (MQ=255)

                            cTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGa                                                             >  1:2772625/1‑93 (MQ=255)

                            cTGCCATTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCGGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGa                                                             <  2:373794/93‑1 (MQ=255)

                             tGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGCACGCCGGAGGTGaa                                                            >  2:1941953/1‑93 (MQ=255)

                             tGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGaa                                                            <  1:2516204/93‑1 (MQ=255)

                             tGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGaa                                                            <  1:5783733/93‑1 (MQ=255)

                             tGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGaa                                                            >  2:3085882/1‑93 (MQ=255)

                               cccTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGaaga                                                          <  2:5344105/93‑3 (MQ=255)

                               cccTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAaca                                                          >  1:500148/1‑93 (MQ=255)

                               cccTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAaca                                                          <  1:4114691/93‑1 (MQ=255)

                                ccTTTCCATCCGAGCAATCACGTCCGGTGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAacac                                                         <  2:2547637/93‑1 (MQ=255)

                                ccTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAacac                                                         >  1:2079674/1‑93 (MQ=255)

                                ccTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGCGAacac                                                         >  1:5834633/1‑93 (MQ=255)

                                ccTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAacac                                                         <  2:2580763/93‑1 (MQ=255)

                                   ttCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTAc                                                      >  1:997100/1‑93 (MQ=255)

                                    tCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTAcg                                                     <  2:6431138/93‑1 (MQ=255)

                                      cATCCGAGCAATCACGTCCGGGGGGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTAcgcg                                                   >  1:6104459/1‑93 (MQ=255)

                                       aTCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCTATCATCACTAATGGAACGCCGCAGGCGAACACTAcgcgc                                                  >  1:1431969/1‑92 (MQ=255)

                                       aTCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGt                                                  >  1:3853718/1‑93 (MQ=255)

                                       aTCCGAGCAATCACGTCCGGGGTGCAAACGGCCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGt                                                  <  2:4615219/93‑1 (MQ=255)

                                        tCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTg                                                 <  1:2408227/93‑1 (MQ=255)

                                          cGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCg                                               >  1:3223859/1‑93 (MQ=255)

                                            aGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGc                                             <  1:1642579/93‑1 (MQ=255)

                                            aGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGc                                             <  1:3997856/93‑1 (MQ=255)

                                             gCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCt                                            >  1:6045116/1‑93 (MQ=255)

                                             gCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCt                                            <  1:3338534/93‑1 (MQ=255)

                                             gCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCt                                            <  1:2319749/93‑1 (MQ=255)

                                                aTCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTcc                                         >  2:32982/1‑93 (MQ=255)

                                                 tCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCg                                        <  2:4889901/93‑1 (MQ=255)

                                                  cACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCgg                                       <  1:1902066/93‑1 (MQ=255)

                                                   aCGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCggg                                      >  1:2655187/1‑93 (MQ=255)

                                                    cGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGt                                     >  1:2292147/1‑93 (MQ=255)

                                                    cGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGt                                     <  2:6229683/93‑1 (MQ=255)

                                                     gTCCGGGGTGCAAACGGTCTGTTTGGTGAGATCGAGGGCCATCATCACTAATGGATCGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTc                                    <  2:784484/93‑1 (MQ=255)

                                                     gTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTc                                    <  1:2246770/93‑1 (MQ=255)

                                                      tCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCggtggg                                   >  2:3159885/1‑89 (MQ=255)

                                                      tCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCg                                   >  1:1568776/1‑93 (MQ=255)

                                                      gccGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGACCGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCg                                   <  1:5565034/92‑1 (MQ=255)

                                                        cGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGc                                 <  1:6164471/93‑1 (MQ=255)

                                                        cGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGc                                 >  1:2922611/1‑93 (MQ=255)

                                                         ggggTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCa                                <  1:4043062/93‑1 (MQ=255)

                                                         ggggTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTACGGGTCGGCa                                >  1:3253453/1‑93 (MQ=255)

                                                          gggTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGCGAACACTACGCGTGCGGCTTCCGGGTCGGCaa                               >  1:1756516/1‑93 (MQ=255)

                                                           ggTGCAAACGGTCTGGTGGGTGAGATCGAGGGCCACCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCaaa                              <  1:1093433/93‑1 (MQ=255)

                                                            gTGCAGACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGTTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAg                             <  1:273155/93‑1 (MQ=255)

                                                            gTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAg                             <  1:4853049/93‑1 (MQ=255)

                                                             tGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGa                            >  1:6325950/1‑93 (MQ=255)

                                                              gCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGAt                           >  2:2619206/1‑93 (MQ=255)

                                                               cAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATg                          >  2:210034/1‑93 (MQ=255)

                                                               cAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATg                          >  1:1293658/1‑93 (MQ=255)

                                                                 aaCGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGtt                        >  2:3147884/1‑93 (MQ=255)

                                                                 aaCGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGtt                        >  2:5657471/1‑93 (MQ=255)

                                                                  aCGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGCTGTTg                       >  2:3947091/1‑93 (MQ=255)

                                                                  aCGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTg                       >  2:4365853/1‑93 (MQ=255)

                                                                   cGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTTAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGa                      <  1:3036961/93‑1 (MQ=255)

                                                                    ggTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGaa                     <  1:1225487/93‑1 (MQ=255)

                                                                    ggTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGaa                     >  1:1387468/1‑93 (MQ=255)

                                                                    ggTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGaa                     <  2:5305491/93‑1 (MQ=255)

                                                                     gTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAAc                    >  1:5233739/1‑93 (MQ=255)

                                                                     gTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAAc                    <  1:2511836/93‑1 (MQ=255)

                                                                      tCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACt                   <  1:741768/93‑1 (MQ=255)

                                                                          gttggtGAGATCGAGTGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGc               <  1:3530180/93‑1 (MQ=255)

                                                                           ttggtGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCa              >  1:856336/1‑93 (MQ=255)

                                                                             ggtGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGa            <  1:5974976/93‑1 (MQ=255)

                                                                              gtGAGATCCAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGATTCCGGGTCGGCAAAGATGTTGAACTCGGCAggt           >  2:5554227/1‑91 (MQ=255)

                                                                                  gaTCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCg       <  2:4789803/93‑1 (MQ=255)

                                                                                    tCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTg     >  1:5373042/1‑93 (MQ=255)

                                                                                    tCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCCGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTg     >  2:3587497/1‑93 (MQ=255)

                                                                                     cGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGa    <  2:4131379/93‑1 (MQ=255)

                                                                                     cGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGa    <  2:492577/93‑1 (MQ=255)

                                                                                     cGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGa    <  1:5091202/93‑1 (MQ=255)

                                                                                      gAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGaa   <  1:432537/93‑1 (MQ=255)

                                                                                      gAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGaa   <  2:3354879/93‑1 (MQ=255)

                                                                                      gAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGAGaa   >  1:3093042/1‑93 (MQ=255)

                                                                                       aGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAg  <  2:5731895/93‑1 (MQ=255)

TCGCTGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAG  >  NC_000913/2252554‑2252733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTCGCTGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGC  >  NC_000913/2252552‑2252737

TGTCGCTGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGC                                                                                              <  SRR4302537.738828/94‑1 (MQ=60)

 GTCGCTGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCC                                                                                             >  SRR4302537.2433199/1‑94 (MQ=60)

   CGCTGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGTGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCAT                                                                                           >  SRR4302537.4587565/1‑94 (MQ=60)

     CTGAACAACTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCA                                                                                         >  SRR4302537.2342589/1‑94 (MQ=60)

      TGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCAT                                                                                        >  SRR4302537.646961/1‑94 (MQ=60)

         ACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCAC                                                                                     >  SRR4302537.2664679/1‑94 (MQ=60)

          CAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACT                                                                                    <  SRR4302537.5247043/94‑1 (MQ=60)

          CAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACT                                                                                    <  SRR4302537.4122377/94‑1 (MQ=60)

           AGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTA                                                                                   >  SRR4302537.3255250/1‑94 (MQ=60)

           AGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTA                                                                                   >  SRR4302537.4913323/1‑94 (MQ=60)

            GCTCTCCGGCGGGGCTGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAA                                                                                  <  SRR4302537.2038534/94‑1 (MQ=60)

            GCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAA                                                                                  >  SRR4302537.2190696/1‑94 (MQ=60)

             CTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAAT                                                                                 >  SRR4302537.5173045/1‑94 (MQ=60)

              TCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATG                                                                                <  SRR4302537.8320/94‑1 (MQ=60)

              TCGCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATG                                                                                <  SRR4302537.2764196/94‑1 (MQ=60)

                 CCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAA                                                                             <  SRR4302537.1289781/94‑1 (MQ=60)

                  CGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTTAGATCGAGGGCCATCATCACTAATGGAAC                                                                            <  SRR4302537.5198560/94‑1 (MQ=60)

                  CGGCGGGCCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAAC                                                                            <  SRR4302537.1216739/94‑1 (MQ=60)

                   GGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACG                                                                           >  SRR4302537.5761371/1‑94 (MQ=60)

                   GGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACG                                                                           >  SRR4302537.3239835/1‑94 (MQ=60)

                     CGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCC                                                                         >  SRR4302537.4001457/1‑94 (MQ=60)

                      GGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCG                                                                        <  SRR4302537.3204469/94‑1 (MQ=60)

                      GGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCG                                                                        >  SRR4302537.3750289/1‑94 (MQ=60)

                       GGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGG                                                                       <  SRR4302537.2293681/94‑1 (MQ=60)

                        GGCCGCCTGCCCTTTCCATCCGATCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGA                                                                      >  SRR4302537.5720702/1‑94 (MQ=60)

                         GCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAG                                                                     <  SRR4302537.4706367/94‑1 (MQ=60)

                          CCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGG                                                                    >  SRR4302537.6100659/1‑94 (MQ=60)

                          CCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGG                                                                    >  SRR4302537.3327234/1‑94 (MQ=60)

                           CGCCTTCCCTTTCCATCCGAGCAATCTCGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGT                                                                   <  SRR4302537.1634279/94‑1 (MQ=60)

                           CGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGT                                                                   >  SRR4302537.142903/1‑94 (MQ=60)

                           CGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGT                                                                   >  SRR4302537.2018303/1‑94 (MQ=60)

                            GCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTG                                                                  <  SRR4302537.1031074/94‑1 (MQ=60)

                            GCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTG                                                                  >  SRR4302537.4935978/1‑94 (MQ=60)

                             CCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGA                                                                 >  SRR4302537.2792002/1‑94 (MQ=60)

                               TGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAAC                                                               <  SRR4302537.2533885/94‑1 (MQ=60)

                               TGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAAC                                                               <  SRR4302537.5823384/94‑1 (MQ=60)

                                GCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACA                                                              >  SRR4302537.503589/1‑94 (MQ=60)

                                 CCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACAC                                                             >  SRR4302537.2094321/1‑94 (MQ=60)

                                 CCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACAC                                                             <  SRR4302537.4143434/94‑1 (MQ=60)

                                 CCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGCGAACAC                                                             >  SRR4302537.5874623/1‑94 (MQ=60)

                                  CCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGATGGCCATCATCACTAATGGAACGCCGGAGGTGAACACT                                                            <  SRR4302537.6188979/94‑1 (MQ=60)

                                   CTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTA                                                           >  SRR4302537.4048443/1‑94 (MQ=60)

                                    TTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTAC                                                          <  SRR4302537.451058/94‑1 (MQ=60)

                                    TTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTAC                                                          >  SRR4302537.1003983/1‑94 (MQ=60)

                                    TTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTAC                                                          >  SRR4302537.3197625/1‑94 (MQ=60)

                                       CCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCG                                                       <  SRR4302537.5815032/94‑1 (MQ=60)

                                       CCATCCGAGCAATCACGTCCGGGGGGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCG                                                       >  SRR4302537.6146399/1‑94 (MQ=60)

                                        CATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCTATCATCACTAATGGAACGCCGCAGGCGAACACTACGCGC                                                      >  SRR4302537.1441935/1‑94 (MQ=60)

                                        CATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGT                                                      >  SRR4302537.3880698/1‑94 (MQ=60)

                                         ATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTG                                                     >  SRR4302537.1956778/1‑94 (MQ=60)

                                         ATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTG                                                     <  SRR4302537.4808351/94‑1 (MQ=60)

                                          TCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGC                                                    <  SRR4302537.2425179/94‑1 (MQ=60)

                                           CCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCG                                                   >  SRR4302537.3246548/1‑94 (MQ=60)

                                              AGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCT                                                >  SRR4302537.4128604/1‑94 (MQ=60)

                                              AGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCT                                                <  SRR4302537.1654082/94‑1 (MQ=60)

                                              AGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCT                                                >  SRR4302537.6086633/1‑94 (MQ=60)

                                              AGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCT                                                <  SRR4302537.4025846/94‑1 (MQ=60)

                                               GCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTT                                               <  SRR4302537.3362170/94‑1 (MQ=60)

                                               GCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTT                                               <  SRR4302537.4309115/94‑1 (MQ=60)

                                               GCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTT                                               <  SRR4302537.6325174/94‑1 (MQ=60)

                                               GCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTT                                               <  SRR4302537.2336132/94‑1 (MQ=60)

                                                 AATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCC                                             >  SRR4302537.3590936/1‑94 (MQ=60)

                                                  ATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCG                                            <  SRR4302537.3460816/94‑1 (MQ=60)

                                                  ATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCG                                            >  SRR4302537.2818561/1‑94 (MQ=60)

                                                   TCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGG                                           <  SRR4302537.5568714/94‑1 (MQ=60)

                                                   ACACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGG                                           <  SRR4302537.4701307/94‑1 (MQ=60)

                                                    CACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGG                                          <  SRR4302537.1530506/94‑1 (MQ=60)

                                                    CACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGG                                          >  SRR4302537.2673747/1‑94 (MQ=60)

                                                    CACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGG                                          <  SRR4302537.1915278/94‑1 (MQ=60)

                                                     ACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGT                                         >  SRR4302537.2308329/1‑94 (MQ=60)

                                                     ACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGT                                         >  SRR4302537.3114314/1‑94 (MQ=60)

                                                       GTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCG                                       <  SRR4302537.2262625/94‑1 (MQ=60)

                                                       GTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCG                                       >  SRR4302537.1579722/1‑94 (MQ=60)

                                                        GCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGACCGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGG                                      <  SRR4302537.5603068/94‑1 (MQ=60)

                                                         CCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGC                                     >  SRR4302537.2943143/1‑94 (MQ=60)

                                                          CGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCA                                    <  SRR4302537.6206906/94‑1 (MQ=60)

                                                          CGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTACGGGTCGGCA                                    >  SRR4302537.3276398/1‑94 (MQ=60)

                                                           GGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAA                                   <  SRR4302537.4953270/94‑1 (MQ=60)

                                                           GGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAA                                   <  SRR4302537.4071335/94‑1 (MQ=60)

                                                           GGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGCGAACACTACGCGTGCGGCTTCCGGGTCGGCAA                                   >  SRR4302537.1768757/1‑94 (MQ=60)

                                                           GGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAA                                   >  SRR4302537.6008177/1‑94 (MQ=60)

                                                             GGTGCAAACGGTCTGGTGGGTGAGATCGAGGGCCACCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAG                                 <  SRR4302537.1100984/94‑1 (MQ=60)

                                                              GTGCAGACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGTTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGA                                <  SRR4302537.275078/94‑1 (MQ=60)

                                                              GTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGA                                <  SRR4302537.4886530/94‑1 (MQ=60)

                                                              GTGCAAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGA                                >  SRR4302537.6369218/1‑94 (MQ=60)

                                                                GCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATG                              >  SRR4302537.1302650/1‑94 (MQ=60)

                                                                  AAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTT                            >  SRR4302537.6383411/1‑94 (MQ=60)

                                                                  AAACGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTT                            >  SRR4302537.5838831/1‑94 (MQ=60)

                                                                  AAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGAAGTT                            >  SRR4302537.6518719/1‑94 (MQ=60)

                                                                     CGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTTAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAA                         <  SRR4302537.3058128/94‑1 (MQ=60)

                                                                     CGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAA                         >  SRR4302537.1397142/1‑94 (MQ=60)

                                                                     CGGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAA                         <  SRR4302537.137154/94‑1 (MQ=60)

                                                                      GGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAAC                        <  SRR4302537.1234003/94‑1 (MQ=60)

                                                                      GGTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAAC                        >  SRR4302537.5269743/1‑94 (MQ=60)

                                                                       GTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACT                       >  SRR4302537.917150/1‑94 (MQ=60)

                                                                       GTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACT                       <  SRR4302537.2529498/94‑1 (MQ=60)

                                                                       GTCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACT                       >  SRR4302537.2693582/1‑94 (MQ=60)

                                                                        TCTGGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTC                      <  SRR4302537.746833/94‑1 (MQ=60)

                                                                          TGGTTGGTGAGAACGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGG                    <  SRR4302537.531861/94‑1 (MQ=60)

                                                                           GGTTGGTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGC                   <  SRR4302537.3027332/94‑1 (MQ=60)

                                                                            GTTGGTGAGATCGAGTGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCA                  <  SRR4302537.3555138/94‑1 (MQ=60)

                                                                            GTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCA                  >  SRR4302537.862213/1‑94 (MQ=60)

                                                                               GGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGAT               <  SRR4302537.6015994/94‑1 (MQ=60)

                                                                                GTGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATG              >  SRR4302537.1752811/1‑94 (MQ=60)

                                                                                 TGAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGG             <  SRR4302537.3258550/94‑1 (MQ=60)

                                                                                 TGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGATGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGG             >  SRR4302537.37659/1‑94 (MQ=60)

                                                                                  GAGATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGGGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGC            <  SRR4302537.2317487/94‑1 (MQ=60)

                                                                                     ATCGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTG         >  SRR4302537.5409792/1‑94 (MQ=60)

                                                                                       CGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAA       <  SRR4302537.5126255/94‑1 (MQ=60)

                                                                                       CGAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGAGAA       >  SRR4302537.3114616/1‑94 (MQ=60)

                                                                                        GAGGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAG      <  SRR4302537.435550/94‑1 (MQ=60)

                                                                                          GGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTT    >  SRR4302537.2633175/1‑94 (MQ=60)

                                                                                          GGGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTT    >  SRR4302537.895760/1‑94 (MQ=60)

                                                                                           GGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTG   <  SRR4302537.3414446/94‑1 (MQ=60)

                                                                                           GGCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTG   <  SRR4302537.5808897/94‑1 (MQ=60)

                                                                                            GCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGC  >  SRR4302537.470108/1‑94 (MQ=60)

TGTCGCTGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGCCCATCATCACTAATGGAACGCCGGAGGTGAACACTACGCGTGCGGCTTCCGGGTCGGCAAAGATGTTGAACTCGGCAGATGGCGTGAAGTTGC  >  NC_000913/2252552‑2252737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`