Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,260,454	A→G	G354G (GGT→GGC)	fruB ←	PTS system, fructose‑specific IIA/fpr component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,260,454	0	A	G	96.7%	680.2 / 5.2	215	G354G (GGT→GGC)	fruB	PTS system, fructose‑specific IIA/fpr component
Reads supporting (aligned to +/- strand): ref base A (5/2); new base G (116/92); total (121/94)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 223 total aligned reads displayed.

ATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACC  >  NC_000913/2260363‑2260546

aTAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGGCCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGcc                                                                                            <  1:7446900/94‑1 (MQ=255)

aTAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGcc                                                                                            >  2:7855757/1‑94 (MQ=255)

  agtagCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCt                                                                                          <  1:5712159/94‑1 (MQ=255)

  agtagCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCt                                                                                          <  1:3180461/94‑1 (MQ=255)

  agtagCAACACGTCCGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCt                                                                                          <  2:3238004/94‑1 (MQ=255)

     agCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTggg                                                                                       >  2:10374410/1‑94 (MQ=255)

     agCAACACGTCTGCTCATTATGCGCCCTCACCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTggg                                                                                       <  1:9685019/94‑1 (MQ=255)

       cAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCg                                                                                     >  1:7090249/1‑94 (MQ=255)

        aaCGCGCCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCgg                                                                                    <  1:5519292/94‑1 (MQ=255)

         acacGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGt                                                                                   >  2:7628178/1‑94 (MQ=255)

         acacGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGt                                                                                   >  2:10017097/1‑94 (MQ=255)

          cacGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTa                                                                                  <  2:2634518/94‑1 (MQ=255)

           acGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGACTGTTCAGCATCTGCGCCCCGGGCGGTaa                                                                                 >  2:5235278/1‑94 (MQ=255)

            cGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTaaa                                                                                >  2:6814662/1‑94 (MQ=255)

            cGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTaaa                                                                                >  2:9495603/1‑94 (MQ=255)

             gTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAg                                                                               >  2:6896309/1‑94 (MQ=255)

             gTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAg                                                                               <  1:5070304/94‑1 (MQ=255)

             gTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAg                                                                               >  1:3790762/1‑94 (MQ=255)

             gTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAg                                                                               >  1:7425075/1‑94 (MQ=255)

               cTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCg                                                                             >  1:1166148/1‑94 (MQ=255)

                tGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGt                                                                            <  2:2983318/94‑1 (MQ=255)

                 gCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTa                                                                           >  1:5953535/1‑94 (MQ=255)

                  cTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAg                                                                          <  1:6174008/94‑1 (MQ=255)

                   tCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTacg                                                                         <  1:6469934/94‑3 (MQ=255)

                   tCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAgg                                                                         <  2:2612527/94‑1 (MQ=255)

                   tCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAgg                                                                         <  1:6420012/94‑1 (MQ=255)

                     aTTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCg                                                                       <  1:448454/94‑1 (MQ=255)

                     aTTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCg                                                                       >  2:4355531/1‑94 (MQ=255)

                       tATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGAt                                                                     >  2:1323193/1‑94 (MQ=255)

                       tATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGAt                                                                     >  1:1043624/1‑94 (MQ=255)

                        aTGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATg                                                                    >  1:6745434/1‑94 (MQ=255)

                         tGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGa                                                                   >  1:2973777/1‑94 (MQ=255)

                         tGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCAAATGCTTTCAGCGCCTGTACAGCATCTGCAACCTGGGCGGTAAAGCGTAGGCGATGa                                                                   <  1:9370273/94‑1 (MQ=255)

                            gcCCTCCCCAAGACCAGCAGCGATAGCGTCACCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCt                                                                <  1:10070426/94‑1 (MQ=255)

                             cccTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCtt                                                               >  1:4432242/1‑94 (MQ=255)

                             cccTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCtt                                                               >  2:8517316/1‑94 (MQ=255)

                              ccTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCttt                                                              >  1:9982671/1‑94 (MQ=255)

                               cTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTc                                                             <  1:3536059/94‑1 (MQ=255)

                               cTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTc                                                             <  1:6896309/94‑1 (MQ=255)

                               cTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTc                                                             >  1:6525703/1‑94 (MQ=255)

                                 ccccAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCtt                                                           <  2:2510678/94‑1 (MQ=255)

                                   ccAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTaa                                                         >  1:922438/1‑94 (MQ=255)

                                    cAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTaag                                                        >  1:2949378/1‑93 (MQ=255)

                                    cAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAAc                                                        >  1:9566092/1‑94 (MQ=255)

                                    cAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAAc                                                        <  1:10324433/94‑1 (MQ=255)

                                      agcccAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGc                                                      <  1:10484238/91‑1 (MQ=255)

                                      aGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGc                                                      >  1:2151016/1‑94 (MQ=255)

                                      aGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGc                                                      >  2:8825271/1‑94 (MQ=255)

                                       gACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGcc                                                     >  1:8484243/1‑94 (MQ=255)

                                       gACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGcc                                                     <  2:8170779/94‑1 (MQ=255)

                                         ccAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCaa                                                   >  1:1972029/1‑94 (MQ=255)

                                           agcagcGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGt                                                 <  1:1471980/94‑1 (MQ=255)

                                              agcGATCGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGcc                                              <  1:8283799/94‑1 (MQ=255)

                                              agcGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGcc                                              >  1:6807263/1‑94 (MQ=255)

                                               gcGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCca                                             <  2:5604270/94‑1 (MQ=255)

                                                cGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCcac                                            >  1:5836166/1‑94 (MQ=255)

                                                cGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCcac                                            >  2:6092458/1‑94 (MQ=255)

                                                cGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCcac                                            >  1:8995613/1‑94 (MQ=255)

                                                 gATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCcaca                                           >  1:1638212/1‑94 (MQ=255)

                                                 gATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCcaca                                           >  2:10549019/1‑94 (MQ=255)

                                                  aTAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACaa                                          >  1:1427399/1‑94 (MQ=255)

                                                   tAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAAc                                         >  1:5373545/1‑94 (MQ=255)

                                                    aGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACt                                        >  2:8682321/1‑94 (MQ=255)

                                                    aGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACt                                        <  2:6414960/94‑1 (MQ=255)

                                                    aGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACt                                        >  2:3646162/1‑94 (MQ=255)

                                                    aGCATCGCCGAGTGCTTGCAGCGCCTGTTCAGCATCTGCGCCCGGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACt                                        <  1:6740645/94‑1 (MQ=255)

                                                      cGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACttt                                      >  1:871211/1‑94 (MQ=255)

                                                       gTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtc                                     >  1:2740456/1‑94 (MQ=255)

                                                         cGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcat                                   <  1:1300167/94‑1 (MQ=255)

                                                         cGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcat                                   <  2:5027071/94‑1 (MQ=255)

                                                         cGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcat                                   >  1:3309588/1‑94 (MQ=255)

                                                          gCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatc                                  >  2:7722827/1‑94 (MQ=255)

                                                           ccGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatca                                 <  2:3265407/94‑1 (MQ=255)

                                                           ccGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatca                                 <  2:10268048/94‑1 (MQ=255)

                                                           ccGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatca                                 >  2:9768637/1‑94 (MQ=255)

                                                            cGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAg                                >  2:4162139/1‑94 (MQ=255)

                                                            cGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAg                                <  1:7526945/94‑1 (MQ=255)

                                                             gATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGa                               >  1:8909878/1‑94 (MQ=255)

                                                               ttGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACt                             <  1:3642914/94‑1 (MQ=255)

                                                               ttGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACt                             >  2:7410109/1‑94 (MQ=255)

                                                                  cTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGGGCCACAACTTTCATCAGACTACg                          >  1:5107591/1‑94 (MQ=255)

                                                                    ttCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTc                        <  2:93479/94‑1 (MQ=255)

                                                                       aGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgt                     >  1:440841/1‑94 (MQ=255)

                                                                       aGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgt                     >  2:1799161/1‑94 (MQ=255)

                                                                       aGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgt                     >  1:8038017/1‑94 (MQ=255)

                                                                         cgcCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgttt                   >  1:2931249/1‑94 (MQ=255)

                                                                         cgcCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgttt                   <  2:5858739/94‑1 (MQ=255)

                                                                          gcCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttg                  <  1:1863904/94‑1 (MQ=255)

                                                                          gcCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttg                  >  1:4274137/1‑94 (MQ=255)

                                                                           ccTGTTCAGCATCTGCGCCTTGGGCGTTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgc                 <  1:971834/94‑1 (MQ=255)

                                                                           ccTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgc                 >  2:6358888/1‑94 (MQ=255)

                                                                            cTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgcc                >  1:1061433/1‑94 (MQ=255)

                                                                             tGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccg               <  1:401235/94‑1 (MQ=255)

                                                                                tCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtt            >  2:8963317/1‑94 (MQ=255)

                                                                                 cAGCATCTGCGCCGGGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggttt           <  2:4952058/94‑1 (MQ=255)

                                                                                 cAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggttt           <  1:4869006/94‑1 (MQ=255)

                                                                                 cAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggttt           <  1:10543035/94‑1 (MQ=255)

                                                                                  aGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtttg          >  2:9306080/1‑94 (MQ=255)

                                                                                  aGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtttg          >  1:8570043/1‑94 (MQ=255)

                                                                                   gCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtttgc         >  2:5104421/1‑94 (MQ=255)

ATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACC  >  NC_000913/2260363‑2260546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACC  >  NC_000913/2260361‑2260546

TGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCG                                                                                              <  SRR4302545.2554997/94‑1 (MQ=60)

  ATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGGCCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCC                                                                                            <  SRR4302545.7657745/94‑1 (MQ=60)

  ATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCC                                                                                            <  SRR4302545.6919431/94‑1 (MQ=60)

  ATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCC                                                                                            <  SRR4302545.3223620/94‑1 (MQ=60)

  ATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCC                                                                                            <  SRR4302545.7657744/94‑1 (MQ=60)

  ATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCC                                                                                            >  SRR4302545.2414167/1‑94 (MQ=60)

    AGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCT                                                                                          <  SRR4302545.3258497/94‑1 (MQ=60)

    AGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCT                                                                                          <  SRR4302545.5865285/94‑1 (MQ=60)

     GTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTG                                                                                         <  SRR4302545.6927555/94‑1 (MQ=60)

       AGCAACACGTCTGCTCATTATGCGCCCTCACCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGG                                                                                       <  SRR4302545.9972630/94‑1 (MQ=60)

        GCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGC                                                                                      <  SRR4302545.7701599/94‑1 (MQ=60)

         CAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCG                                                                                     >  SRR4302545.9894219/1‑94 (MQ=60)

         CAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCG                                                                                     >  SRR4302545.7286584/1‑94 (MQ=60)

         CAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCG                                                                                     >  SRR4302545.9719139/1‑94 (MQ=60)

          AACGCGCCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGG                                                                                    <  SRR4302545.5664936/94‑1 (MQ=60)

          AACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGG                                                                                    >  SRR4302545.5642488/1‑94 (MQ=60)

          AACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGG                                                                                    >  SRR4302545.894879/1‑94 (MQ=60)

           ACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTCGGCGGT                                                                                   <  SRR4302545.10834963/94‑1 (MQ=60)

            CCCGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTA                                                                                  <  SRR4302545.9017833/94‑1 (MQ=60)

             ACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAA                                                                                 <  SRR4302545.8789293/94‑1 (MQ=60)

              CGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAA                                                                                <  SRR4302545.1638308/94‑1 (MQ=60)

               GTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAG                                                                               >  SRR4302545.3884651/1‑94 (MQ=60)

               GTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAG                                                                               >  SRR4302545.6561708/1‑94 (MQ=60)

               GTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAG                                                                               <  SRR4302545.5199457/94‑1 (MQ=60)

               GTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAG                                                                               <  SRR4302545.5199456/94‑1 (MQ=60)

               GTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAG                                                                               >  SRR4302545.7635020/1‑94 (MQ=60)

                TCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGC                                                                              <  SRR4302545.8710732/94‑1 (MQ=60)

                 CTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCG                                                                             <  SRR4302545.1484381/94‑1 (MQ=60)

                 CTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCG                                                                             >  SRR4302545.313280/1‑94 (MQ=60)

                 CTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCG                                                                             >  SRR4302545.1191920/1‑94 (MQ=60)

                  TGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGT                                                                            <  SRR4302545.10791830/94‑1 (MQ=60)

                  TGCTCATTATGCGCCCCCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGT                                                                            >  SRR4302545.3698130/1‑94 (MQ=60)

                   GCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTA                                                                           >  SRR4302545.6114155/1‑94 (MQ=60)

                   GCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTA                                                                           >  SRR4302545.6114157/1‑94 (MQ=60)

                    CTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAG                                                                          <  SRR4302545.10920873/94‑1 (MQ=60)

                    CTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAG                                                                          <  SRR4302545.1186984/94‑1 (MQ=60)

                    CTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAG                                                                          <  SRR4302545.2352700/94‑1 (MQ=60)

                    CTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAG                                                                          <  SRR4302545.6342060/94‑1 (MQ=60)

                     TCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGG                                                                         <  SRR4302545.6595549/94‑1 (MQ=60)

                     TCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGG                                                                         <  SRR4302545.6595550/94‑1 (MQ=60)

                     TCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTACG                                                                         <  SRR4302545.6646811/94‑1 (MQ=60)

                      CATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGC                                                                        <  SRR4302545.8629028/94‑1 (MQ=60)

                      CATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGC                                                                        >  SRR4302545.5236559/1‑94 (MQ=60)

                      CATAATGCGCCCCCCCCAAGACCAGCAGCGAGAGCGTCGACGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGC                                                                        <  SRR4302545.6860334/94‑1 (MQ=60)

                       ATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCG                                                                       <  SRR4302545.458398/94‑1 (MQ=60)

                         TATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGAT                                                                     <  SRR4302545.4425292/94‑1 (MQ=60)

                         TATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGAT                                                                     >  SRR4302545.1066398/1‑94 (MQ=60)

                          ATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATG                                                                    >  SRR4302545.6930405/1‑94 (MQ=60)

                           TGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGA                                                                   >  SRR4302545.3046230/1‑94 (MQ=60)

                           TGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCAAATGCTTTCAGCGCCTGTACAGCATCTGCAACCTGGGCGGTAAAGCGTAGGCGATGA                                                                   <  SRR4302545.9645191/94‑1 (MQ=60)

                            GCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGAC                                                                  <  SRR4302545.6225694/94‑1 (MQ=60)

                              GCCCTCCCCAAGACCAGCAGCGATAGCGTCACCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCT                                                                <  SRR4302545.10368409/94‑1 (MQ=60)

                               CCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTT                                                               >  SRR4302545.4544957/1‑94 (MQ=60)

                               CCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTT                                                               >  SRR4302545.4544956/1‑94 (MQ=60)

                                CCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTT                                                              <  SRR4302545.8403507/94‑1 (MQ=60)

                                CCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTT                                                              >  SRR4302545.10278732/1‑94 (MQ=60)

                                CCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTT                                                              <  SRR4302545.10632129/94‑1 (MQ=60)

                                 CTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTC                                                             <  SRR4302545.7086127/94‑1 (MQ=60)

                                 CTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTC                                                             >  SRR4302545.6704216/1‑94 (MQ=60)

                                 CTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTC                                                             <  SRR4302545.3622656/94‑1 (MQ=60)

                                 CTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTC                                                             >  SRR4302545.4588000/1‑94 (MQ=60)

                                    CCCAAGACCAGCAGGAATAGCGTCGCCGATTGCTTCCAGCCCCTGTTCAGCATATGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTA                                                          <  SRR4302545.8782411/94‑1 (MQ=60)

                                     CCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAA                                                         >  SRR4302545.943141/1‑94 (MQ=60)

                                      CAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAAG                                                        >  SRR4302545.3021154/1‑94 (MQ=60)

                                      CAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAAC                                                        <  SRR4302545.1493232/94‑1 (MQ=60)

                                      CAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAAC                                                        >  SRR4302545.21235/1‑94 (MQ=60)

                                      CAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAAC                                                        >  SRR4302545.9848073/1‑94 (MQ=60)

                                      CAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAAC                                                        <  SRR4302545.10628849/94‑1 (MQ=60)

                                       AAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACG                                                       <  SRR4302545.4608716/94‑1 (MQ=60)

                                        AGCCCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGC                                                      <  SRR4302545.10793292/94‑1 (MQ=60)

                                        AGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGC                                                      >  SRR4302545.2203643/1‑94 (MQ=60)

                                         GACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCC                                                     >  SRR4302545.8729415/1‑94 (MQ=60)

                                         GACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCC                                                     >  SRR4302545.9834197/1‑94 (MQ=60)

                                           CCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAA                                                   >  SRR4302545.2020889/1‑94 (MQ=60)

                                            CAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAG                                                  >  SRR4302545.9487473/1‑94 (MQ=60)

                                             AGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGT                                                 >  SRR4302545.3866790/1‑94 (MQ=60)

                                             AGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGT                                                 <  SRR4302545.1505809/94‑1 (MQ=60)

                                             AGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGT                                                 <  SRR4302545.9973491/94‑1 (MQ=60)

                                                AGCGATCGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCC                                              <  SRR4302545.8523764/94‑1 (MQ=60)

                                                AGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCC                                              >  SRR4302545.3581086/1‑94 (MQ=60)

                                                AGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCC                                              >  SRR4302545.6994316/1‑94 (MQ=60)

                                                  TGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCAC                                            >  SRR4302545.724043/1‑94 (MQ=60)

                                                  CGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCAC                                            >  SRR4302545.5993280/1‑94 (MQ=60)

                                                  CGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCAC                                            >  SRR4302545.9257534/1‑94 (MQ=60)

                                                   GATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACA                                           >  SRR4302545.1677083/1‑94 (MQ=60)

                                                    ATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAA                                          <  SRR4302545.8889945/94‑1 (MQ=60)

                                                    ATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAA                                          >  SRR4302545.1459774/1‑94 (MQ=60)

                                                    ATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAA                                          <  SRR4302545.4114852/94‑1 (MQ=60)

                                                     TAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAAC                                         >  SRR4302545.5513306/1‑94 (MQ=60)

                                                      AGCATCGCCGAGTGCTTGCAGCGCCTGTTCAGCATCTGCGCCCGGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACT                                        <  SRR4302545.6925458/94‑1 (MQ=60)

                                                       GCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTT                                       >  SRR4302545.1423311/1‑94 (MQ=60)

                                                        CGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTT                                      >  SRR4302545.890749/1‑94 (MQ=60)

                                                         GTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTC                                     >  SRR4302545.7021621/1‑94 (MQ=60)

                                                         GTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTC                                     >  SRR4302545.2806552/1‑94 (MQ=60)

                                                         GTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTC                                     <  SRR4302545.2866713/94‑1 (MQ=60)

                                                           CGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCAT                                   >  SRR4302545.3390513/1‑94 (MQ=60)

                                                           CGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCAT                                   <  SRR4302545.1329039/94‑1 (MQ=60)

                                                             CCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCA                                 <  SRR4302545.6043422/94‑1 (MQ=60)

                                                             CCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGACGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCA                                 <  SRR4302545.3022703/94‑1 (MQ=60)

                                                              CGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAG                                >  SRR4302545.10585135/1‑94 (MQ=60)

                                                              CGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAG                                <  SRR4302545.7739884/94‑1 (MQ=60)

                                                              CGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAG                                >  SRR4302545.10095705/1‑94 (MQ=60)

                                                              CGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAG                                <  SRR4302545.5859518/94‑1 (MQ=60)

                                                               GATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGA                               >  SRR4302545.9168873/1‑94 (MQ=60)

                                                                ATTGCTTTCAGCGCCTGTGCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGAC                              <  SRR4302545.10291485/94‑1 (MQ=60)

                                                                 TTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACT                             <  SRR4302545.3731887/94‑1 (MQ=60)

                                                                  TGCTTTCAGCGCCTGTTCAGCATGTGCGCACTGGGCGGTAAAGCGTAGGCGATGCCCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTA                            <  SRR4302545.944925/94‑1 (MQ=60)

                                                                  TGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTA                            <  SRR4302545.4538068/94‑1 (MQ=60)

                                                                   GCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTAC                           >  SRR4302545.7193326/1‑94 (MQ=60)

                                                                   GCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTAC                           >  SRR4302545.8057821/1‑94 (MQ=60)

                                                                    CTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGGGCCACAACTTTCATCAGACTACG                          >  SRR4302545.5238211/1‑94 (MQ=60)

                                                                     TTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGT                         >  SRR4302545.5878267/1‑94 (MQ=60)

                                                                       TCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCC                       >  SRR4302545.7316528/1‑94 (MQ=60)

                                                                         AGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGT                     <  SRR4302545.4081813/94‑1 (MQ=60)

                                                                         AGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGT                     >  SRR4302545.450644/1‑94 (MQ=60)

                                                                         AGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGT                     >  SRR4302545.8267223/1‑94 (MQ=60)

                                                                           CGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTT                   >  SRR4302545.6975873/1‑94 (MQ=60)

                                                                           CGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTT                   >  SRR4302545.7927142/1‑94 (MQ=60)

                                                                           CGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTT                   >  SRR4302545.3002499/1‑94 (MQ=60)

                                                                           CGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTT                   >  SRR4302545.3002498/1‑94 (MQ=60)

                                                                           CGCCTGTTCAGCATCGGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTT                   <  SRR4302545.1609093/94‑1 (MQ=60)

                                                                            GCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGCCCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTG                  <  SRR4302545.5389141/94‑1 (MQ=60)

                                                                            GCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACACCTTTCATCAGACTACGTCCGTTTG                  <  SRR4302545.6699818/94‑1 (MQ=60)

                                                                            GCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTG                  >  SRR4302545.4381483/1‑94 (MQ=60)

                                                                            GCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTG                  <  SRR4302545.1909525/94‑1 (MQ=60)

                                                                             CCTGTTCAGCATCTGCGCCTTGGGCGTTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGC                 <  SRR4302545.993576/94‑1 (MQ=60)

                                                                              CTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCC                >  SRR4302545.1084571/1‑94 (MQ=60)

                                                                               TGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCG               <  SRR4302545.410162/94‑1 (MQ=60)

                                                                               TGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCG               >  SRR4302545.1301301/1‑94 (MQ=60)

                                                                                GTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGG              >  SRR4302545.1964570/1‑94 (MQ=60)

                                                                                GTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGG              <  SRR4302545.9180871/94‑1 (MQ=60)

                                                                                 TTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGT             >  SRR4302545.3387774/1‑94 (MQ=60)

                                                                                 TTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGT             <  SRR4302545.5295805/94‑1 (MQ=60)

                                                                                   CAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTT           <  SRR4302545.10853602/94‑1 (MQ=60)

                                                                                   CAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTT           <  SRR4302545.4992859/94‑1 (MQ=60)

                                                                                    AGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTG          >  SRR4302545.8817886/1‑94 (MQ=60)

                                                                                      CATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCC        <  SRR4302545.10900089/94‑1 (MQ=60)

                                                                                       ATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTGATCAGACTACGTCCGTTTGCCGGTTTGCCG       >  SRR4302545.1857252/1‑94 (MQ=60)

                                                                                        TCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGG      >  SRR4302545.376204/1‑94 (MQ=60)

                                                                                            CGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTCCC  >  SRR4302545.2113795/1‑94 (MQ=60)

TGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACC  >  NC_000913/2260361‑2260546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`