Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,260,454 A→G G354G (GGT→GGC)  fruB ← PTS system, fructose‑specific IIA/fpr component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,260,4540AG100.0% 759.9 / NA 222G354G (GGT→GGC) fruBPTS system, fructose‑specific IIA/fpr component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (119/103);  total (119/103)

Only 100 of 222 total aligned reads displayed.

TAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGT  >  NC_000913/2260364‑2260543
                                                                                          |                                                                                         
tagtagCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGcc                                                                                         <  1:1821477/93‑1 (MQ=255)
  gtagCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCt                                                                                       <  1:28768/93‑1 (MQ=255)
    agCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTgg                                                                                     <  1:1233870/93‑1 (MQ=255)
     gCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTggg                                                                                    >  1:3618661/1‑93 (MQ=255)
      cAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGc                                                                                   <  1:335224/93‑1 (MQ=255)
      cAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGc                                                                                   >  1:650451/1‑93 (MQ=255)
        acacGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCgg                                                                                 >  2:2522944/1‑93 (MQ=255)
          acGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTa                                                                               <  2:1549910/93‑1 (MQ=255)
           cGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTaa                                                                              >  2:5511207/1‑93 (MQ=255)
            gTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTaaa                                                                             >  2:3500255/1‑93 (MQ=255)
            gTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTaaa                                                                             <  1:1670223/93‑1 (MQ=255)
            gTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTaaa                                                                             >  1:6280050/1‑93 (MQ=255)
            gTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTaaa                                                                             <  2:5033404/93‑1 (MQ=255)
             tCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAg                                                                            <  2:1087678/93‑1 (MQ=255)
             tCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAg                                                                            >  1:2484824/1‑93 (MQ=255)
             tCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAg                                                                            >  1:5257746/1‑93 (MQ=255)
                gCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTAAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGt                                                                         <  2:517024/93‑1 (MQ=255)
                 cTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTa                                                                        <  1:4044842/93‑1 (MQ=255)
                  tCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAg                                                                       >  1:1274801/1‑93 (MQ=255)
                   cATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAgg                                                                      <  1:5463634/93‑1 (MQ=255)
                   cATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAgg                                                                      <  2:4176681/93‑1 (MQ=255)
                   cATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAgg                                                                      <  1:3830785/93‑1 (MQ=255)
                   cATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAgg                                                                      <  2:745149/93‑1 (MQ=255)
                   cATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAgg                                                                      >  1:3194180/1‑93 (MQ=255)
                   cATTATCCGCCCCCCCCAAGACGAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAgg                                                                      <  1:4353995/93‑1 (MQ=255)
                    aTTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGc                                                                     <  1:3626670/93‑1 (MQ=255)
                    aTTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGc                                                                     >  1:4472555/1‑93 (MQ=255)
                     ttATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCg                                                                    >  2:5950030/1‑93 (MQ=255)
                      tATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGa                                                                   >  1:1456023/1‑93 (MQ=255)
                      tATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGa                                                                   <  1:5919698/93‑1 (MQ=255)
                      tATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGa                                                                   <  2:4390307/93‑1 (MQ=255)
                       aTGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGAt                                                                  <  1:2751316/93‑1 (MQ=255)
                        tGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATg                                                                 >  1:6202212/1‑93 (MQ=255)
                         gcgcCCTCCCTAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGa                                                                <  1:63930/93‑1 (MQ=255)
                           gcCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGAcc                                                              <  2:2970803/93‑1 (MQ=255)
                           gcCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGAcc                                                              >  2:1735403/1‑93 (MQ=255)
                           gcCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGAcc                                                              <  2:3016216/93‑1 (MQ=255)
                           gcCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGAcc                                                              >  1:3299154/1‑93 (MQ=255)
                            cccTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCt                                                             >  2:6268841/1‑93 (MQ=255)
                             ccTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCtt                                                            <  1:4994272/93‑1 (MQ=255)
                             ccTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCtt                                                            >  1:4501912/1‑93 (MQ=255)
                             ccTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCtt                                                            >  1:3107143/1‑93 (MQ=255)
                             ccTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCtt                                                            <  2:3296338/93‑1 (MQ=255)
                             ccTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCtt                                                            <  1:715243/93‑1 (MQ=255)
                              cTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCttt                                                           >  1:3132018/1‑93 (MQ=255)
                               cccccAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTc                                                          <  1:4883084/92‑1 (MQ=255)
                                ccccAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCt                                                         <  1:914725/93‑1 (MQ=255)
                                  ccAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTa                                                       <  1:2292413/93‑1 (MQ=255)
                                    aaGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAAc                                                     >  2:4297670/1‑93 (MQ=255)
                                      gACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGc                                                   >  2:4064406/1‑93 (MQ=255)
                                       aCCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGcc                                                  <  1:4516978/93‑1 (MQ=255)
                                          agcagcGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAg                                               <  2:952372/93‑1 (MQ=255)
                                           gcagcGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGt                                              <  1:5585985/93‑1 (MQ=255)
                                                 aTAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCcaca                                        >  2:1390856/1‑93 (MQ=255)
                                                 aTAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCcaca                                        <  2:4458633/93‑1 (MQ=255)
                                                 aTAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCcaca                                        >  2:5054511/1‑93 (MQ=255)
                                                   aGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAAc                                      >  2:1765927/1‑93 (MQ=255)
                                                   aGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAAc                                      <  1:2609064/93‑1 (MQ=255)
                                                    gCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACt                                     <  2:5692043/93‑1 (MQ=255)
                                                      gTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACttt                                   <  1:1535193/93‑1 (MQ=255)
                                                       tCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtc                                  <  2:4425593/93‑1 (MQ=255)
                                                       tCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtc                                  >  1:2049886/1‑93 (MQ=255)
                                                        cGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtca                                 <  2:5072747/93‑1 (MQ=255)
                                                        cGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtca                                 >  1:5341842/1‑93 (MQ=255)
                                                        cGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtca                                 >  2:2318285/1‑93 (MQ=255)
                                                         gCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcat                                <  2:2838785/93‑1 (MQ=255)
                                                          cctaTTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatc                               <  2:403290/90‑1 (MQ=255)
                                                          ccGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatc                               <  1:5841905/93‑1 (MQ=255)
                                                           cGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatca                              >  2:576266/1‑93 (MQ=255)
                                                           cGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatca                              <  1:4606719/93‑1 (MQ=255)
                                                           cGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatca                              >  2:2383059/1‑93 (MQ=255)
                                                            gATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAg                             >  1:77615/1‑93 (MQ=255)
                                                            gATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAg                             <  1:1160399/93‑1 (MQ=255)
                                                            gATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAg                             <  2:1920401/93‑1 (MQ=255)
                                                            gATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAg                             >  1:3734617/1‑93 (MQ=255)
                                                              ttGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGAc                           >  2:2027161/1‑93 (MQ=255)
                                                                gCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTa                         >  2:3966123/1‑93 (MQ=255)
                                                                gCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTa                         >  2:6351312/1‑93 (MQ=255)
                                                                 cTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACACCTTTCATCAGACTAc                        >  1:4299696/1‑93 (MQ=255)
                                                                 cTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTAc                        >  2:5582732/1‑93 (MQ=255)
                                                                  tttCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACg                       >  1:4284784/1‑93 (MQ=255)
                                                                   ttCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGt                      <  2:5846794/93‑1 (MQ=255)
                                                                    tCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTc                     <  2:172256/93‑1 (MQ=255)
                                                                       gcgcCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgt                  >  1:2338985/1‑93 (MQ=255)
                                                                       gcgcCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgt                  >  2:2875063/1‑93 (MQ=255)
                                                                       gcgcCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgt                  >  1:6238191/1‑93 (MQ=255)
                                                                          ccTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttg               >  1:1142810/1‑93 (MQ=255)
                                                                          ccTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttg               >  2:4923177/1‑93 (MQ=255)
                                                                           cTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgc              >  2:5475656/1‑93 (MQ=255)
                                                                           cTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgc              >  1:1067736/1‑93 (MQ=255)
                                                                            tGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgcc             >  1:603440/1‑93 (MQ=255)
                                                                            tGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgcc             >  1:3244269/1‑93 (MQ=255)
                                                                             gTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccg            <  2:3878053/93‑1 (MQ=255)
                                                                              ttCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccgg           <  2:3056748/93‑1 (MQ=255)
                                                                                cAGCATCTGCGCCCTGGGCGGTAAAGCTTAGGCGATCACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtt         <  2:3803751/93‑1 (MQ=255)
                                                                                cAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtt         >  2:4752180/1‑93 (MQ=255)
                                                                                   cATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtttgc      <  1:2898982/93‑1 (MQ=255)
                                                                                   cATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtttgc      <  2:4157633/93‑1 (MQ=255)
                                                                                     tCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtttgccg    <  2:5388033/93‑1 (MQ=255)
                                                                                       tGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTttgccggtttgccggt  >  2:212187/1‑93 (MQ=255)
                                                                                          |                                                                                         
TAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGT  >  NC_000913/2260364‑2260543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCA  >  NC_000913/2260361‑2260547
                                                                                             |                                                                                             
TGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCG                                                                                               >  SRR4302537.5462903/1‑94 (MQ=60)
  ATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCC                                                                                             <  SRR4302537.1522102/94‑1 (MQ=60)
   TAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCC                                                                                            <  SRR4302537.1834177/94‑1 (MQ=60)
   TAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCC                                                                                            <  SRR4302537.6286382/94‑1 (MQ=60)
    AGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCT                                                                                           >  SRR4302537.750892/1‑94 (MQ=60)
    AGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCT                                                                                           <  SRR4302537.2665468/94‑1 (MQ=60)
     atagcagcgCGTCTGCTCATTATGCGCCCCCCCCAAGGCCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTG                                                                                          <  SRR4302537.452099/85‑1 (MQ=60)
     GTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTG                                                                                          <  SRR4302537.28998/94‑1 (MQ=60)
      TAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGG                                                                                         >  SRR4302537.4173032/1‑94 (MQ=60)
      TAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGG                                                                                         <  SRR4302537.5877263/94‑1 (MQ=60)
       AGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGG                                                                                        <  SRR4302537.1242451/94‑1 (MQ=60)
       AGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGG                                                                                        >  SRR4302537.3644168/1‑94 (MQ=60)
       AGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGG                                                                                        >  SRR4302537.5633411/1‑94 (MQ=60)
        GCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGC                                                                                       <  SRR4302537.5159921/94‑1 (MQ=60)
        GCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGC                                                                                       >  SRR4302537.654880/1‑94 (MQ=60)
         CAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCG                                                                                      <  SRR4302537.233657/94‑1 (MQ=60)
         CAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCG                                                                                      >  SRR4302537.1482331/1‑94 (MQ=60)
         CAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCG                                                                                      <  SRR4302537.337544/94‑1 (MQ=60)
          AACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGG                                                                                     >  SRR4302537.3241174/1‑94 (MQ=60)
          AACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGG                                                                                     <  SRR4302537.207588/94‑1 (MQ=60)
           ACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGT                                                                                    >  SRR4302537.2841448/1‑94 (MQ=60)
             ACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAA                                                                                  <  SRR4302537.2541986/94‑1 (MQ=60)
              CGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAA                                                                                 >  SRR4302537.6323122/1‑94 (MQ=60)
              CGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAA                                                                                 >  SRR4302537.6323121/1‑94 (MQ=60)
               GTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAG                                                                                <  SRR4302537.3339387/94‑1 (MQ=60)
               GTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAG                                                                                >  SRR4302537.2502317/1‑94 (MQ=60)
               GTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAG                                                                                <  SRR4302537.4679801/94‑1 (MQ=60)
               GTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAG                                                                                >  SRR4302537.5293929/1‑94 (MQ=60)
               GTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAG                                                                                >  SRR4302537.1272577/1‑94 (MQ=60)
               GTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAG                                                                                <  SRR4302537.1681922/94‑1 (MQ=60)
               GTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAG                                                                                <  SRR4302537.4593972/94‑1 (MQ=60)
                  TGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGTATCTGCGCCCTGGGCGGTAAAGCGT                                                                             >  SRR4302537.1364914/1‑94 (MQ=60)
                  TGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGT                                                                             >  SRR4302537.6346824/1‑94 (MQ=60)
                   GCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTA                                                                            >  SRR4302537.4517564/1‑94 (MQ=60)
                    CTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAG                                                                           >  SRR4302537.1300527/1‑94 (MQ=60)
                    CTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAG                                                                           <  SRR4302537.3285173/94‑1 (MQ=60)
                    CTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAG                                                                           <  SRR4302537.3591373/94‑1 (MQ=60)
                    CTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAG                                                                           <  SRR4302537.3154518/94‑1 (MQ=60)
                    CTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAG                                                                           >  SRR4302537.1283667/1‑94 (MQ=60)
                    CTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAG                                                                           <  SRR4302537.4073125/94‑1 (MQ=60)
                    CTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAG                                                                           <  SRR4302537.271382/94‑1 (MQ=60)
                     TCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGG                                                                          >  SRR4302537.3216641/1‑94 (MQ=60)
                      CATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGC                                                                         <  SRR4302537.3857636/94‑1 (MQ=60)
                      CATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGC                                                                         >  SRR4302537.4503496/1‑94 (MQ=60)
                      CATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGC                                                                         >  SRR4302537.1728123/1‑94 (MQ=60)
                      CATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGC                                                                         <  SRR4302537.5500902/94‑1 (MQ=60)
                      CATTATCCGCCCCCCCCAAGACGAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGC                                                                         <  SRR4302537.4384144/94‑1 (MQ=60)
                       ATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCG                                                                        <  SRR4302537.3652235/94‑1 (MQ=60)
                        TTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGA                                                                       >  SRR4302537.1115987/1‑94 (MQ=60)
                        TTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGA                                                                       >  SRR4302537.1466164/1‑94 (MQ=60)
                         TATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGAT                                                                      <  SRR4302537.5960320/94‑1 (MQ=60)
                         TATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGAT                                                                      <  SRR4302537.6131393/94‑1 (MQ=60)
                          ATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATG                                                                     <  SRR4302537.2770513/94‑1 (MQ=60)
                          ATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATG                                                                     <  SRR4302537.2417845/94‑1 (MQ=60)
                          ATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATG                                                                     >  SRR4302537.6244957/1‑94 (MQ=60)
                           TGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCTATGA                                                                    <  SRR4302537.5338509/94‑1 (MQ=60)
                            GCGCCCTCCCTAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGAC                                                                   <  SRR4302537.64405/94‑1 (MQ=60)
                             CGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACC                                                                  >  SRR4302537.3322480/1‑94 (MQ=60)
                               CCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTT                                                                >  SRR4302537.3128844/1‑94 (MQ=60)
                               CCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTT                                                                >  SRR4302537.4533044/1‑94 (MQ=60)
                                CCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTT                                                               >  SRR4302537.1162322/1‑94 (MQ=60)
                                CCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTT                                                               <  SRR4302537.720133/94‑1 (MQ=60)
                                CCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTT                                                               >  SRR4302537.3153993/1‑94 (MQ=60)
                                CCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTT                                                               <  SRR4302537.5028685/94‑1 (MQ=60)
                                 CTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTC                                                              >  SRR4302537.5496796/1‑94 (MQ=60)
                                  TCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCT                                                             >  SRR4302537.4320609/1‑94 (MQ=60)
                                  CCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCT                                                             <  SRR4302537.4916811/94‑1 (MQ=60)
                                   CCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTT                                                            <  SRR4302537.1178310/94‑1 (MQ=60)
                                   CCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTT                                                            <  SRR4302537.921040/94‑1 (MQ=60)
                                     CCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAA                                                          <  SRR4302537.2308595/94‑1 (MQ=60)
                                     CCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAA                                                          >  SRR4302537.775601/1‑94 (MQ=60)
                                       AAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACG                                                        >  SRR4302537.4467954/1‑94 (MQ=60)
                                       AAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACG                                                        <  SRR4302537.1646780/94‑1 (MQ=60)
                                          ACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCA                                                     <  SRR4302537.4548224/94‑1 (MQ=60)
                                           CCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAA                                                    >  SRR4302537.3400142/1‑94 (MQ=60)
                                             AGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGT                                                  >  SRR4302537.2897285/1‑94 (MQ=60)
                                             AGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGT                                                  >  SRR4302537.6018849/1‑94 (MQ=60)
                                              GCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTG                                                 <  SRR4302537.5624176/94‑1 (MQ=60)
                                              GCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTG                                                 <  SRR4302537.5108252/94‑1 (MQ=60)
                                                  CGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCAC                                             >  SRR4302537.3820078/1‑94 (MQ=60)
                                                   GATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACA                                            <  SRR4302537.3650748/94‑1 (MQ=60)
                                                   GATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACA                                            >  SRR4302537.4335359/1‑94 (MQ=60)
                                                      AGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACT                                         <  SRR4302537.2627313/94‑1 (MQ=60)
                                                        CGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTT                                       <  SRR4302537.3906112/94‑1 (MQ=60)
                                                         GTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTC                                      >  SRR4302537.2064266/1‑94 (MQ=60)
                                                         GTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTC                                      <  SRR4302537.1545915/94‑1 (MQ=60)
                                                          TCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCA                                     >  SRR4302537.5378451/1‑94 (MQ=60)
                                                             CCGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCA                                  <  SRR4302537.5881963/94‑1 (MQ=60)
                                                              CGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAG                                 >  SRR4302537.3693643/1‑94 (MQ=60)
                                                              CGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAG                                 >  SRR4302537.3760849/1‑94 (MQ=60)
                                                              CGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAG                                 >  SRR4302537.78183/1‑94 (MQ=60)
                                                              CGATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAG                                 <  SRR4302537.4638618/94‑1 (MQ=60)
                                                               GATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGA                                <  SRR4302537.1168472/94‑1 (MQ=60)
                                                                ATTGCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGAC                               >  SRR4302537.4244947/1‑94 (MQ=60)
                                                                   GCTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACACCTTTCATCAGACTAC                            >  SRR4302537.4329575/1‑94 (MQ=60)
                                                                    CTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACG                           >  SRR4302537.4314569/1‑94 (MQ=60)
                                                                    CTTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACG                           >  SRR4302537.70874/1‑94 (MQ=60)
                                                                     TTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGT                          >  SRR4302537.2056062/1‑94 (MQ=60)
                                                                     TTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGT                          >  SRR4302537.4537219/1‑94 (MQ=60)
                                                                     TTTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGT                          <  SRR4302537.294003/94‑1 (MQ=60)
                                                                      TTCAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTC                         <  SRR4302537.5015591/94‑1 (MQ=60)
                                                                        CAGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCG                       <  SRR4302537.6266443/94‑1 (MQ=60)
                                                                         AGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGT                      >  SRR4302537.2355471/1‑94 (MQ=60)
                                                                         AGCGCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGT                      >  SRR4302537.6281082/1‑94 (MQ=60)
                                                                            GCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTG                   >  SRR4302537.1150754/1‑94 (MQ=60)
                                                                            GCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTG                   >  SRR4302537.6464977/1‑94 (MQ=60)
                                                                            GCCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACCCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTG                   <  SRR4302537.907586/94‑1 (MQ=60)
                                                                             CCTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGC                  >  SRR4302537.1075092/1‑94 (MQ=60)
                                                                              CTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCC                 >  SRR4302537.3267134/1‑94 (MQ=60)
                                                                              CTGTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCC                 >  SRR4302537.607567/1‑94 (MQ=60)
                                                                                GTTCAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGG               <  SRR4302537.3198021/94‑1 (MQ=60)
                                                                                   CAGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTT            >  SRR4302537.5615436/1‑94 (MQ=60)
                                                                                    AGCATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTG           >  SRR4302537.2126720/1‑94 (MQ=60)
                                                                                      CATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCC         <  SRR4302537.2919352/94‑1 (MQ=60)
                                                                                       ATCTGCGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCG        >  SRR4302537.5953171/1‑94 (MQ=60)
                                                                                          TGCGCCCTGGGCGTTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTA     <  SRR4302537.3138414/94‑1 (MQ=60)
                                                                                            CGCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACC   >  SRR4302537.4184292/1‑94 (MQ=60)
                                                                                             GCCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCA  >  SRR4302537.1933915/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCA  >  NC_000913/2260361‑2260547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑