Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,264,392 T→C L43P (CTG→CCG)  yeiQ → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,264,3920TC97.9% 784.8 / 3.4 234L43P (CTG→CCG) yeiQputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (111/118);  total (114/120)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 240 total aligned reads displayed.

TACACGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACTGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCTCC  >  NC_000913/2264302‑2264481
                                                                                          |                                                                                         
   acGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTaccgaccg                                                                                      >  2:3899257/1‑93 (MQ=255)
     gCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGAt                                                                                    <  2:1104744/93‑1 (MQ=255)
     gCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGAt                                                                                    >  2:3146923/1‑93 (MQ=255)
     gCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGAt                                                                                    >  1:5468645/1‑93 (MQ=255)
       tCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCg                                                                                  >  2:2263167/1‑93 (MQ=255)
       tCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCg                                                                                  >  2:3312354/1‑93 (MQ=255)
         cACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCgtg                                                                                >  1:3948165/1‑93 (MQ=255)
         cACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCgtg                                                                                <  2:751953/93‑1 (MQ=255)
          aCGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCgtgt                                                                               >  1:3488911/1‑93 (MQ=255)
           cGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGtgtg                                                                              <  2:2253533/93‑1 (MQ=255)
            gCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACTGACCGATCGtgtgt                                                                             >  1:1432956/1‑92 (MQ=255)
            gCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGc                                                                             <  1:1860717/93‑1 (MQ=255)
            gCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGc                                                                             <  2:5232951/93‑1 (MQ=255)
            gCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGc                                                                             <  1:1410406/93‑1 (MQ=255)
             cTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCt                                                                            <  1:607612/93‑1 (MQ=255)
              tATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTg                                                                           >  1:3525298/1‑93 (MQ=255)
                tGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGaa                                                                         <  1:506687/93‑1 (MQ=255)
                tGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGaa                                                                         >  1:3756840/1‑93 (MQ=255)
                 gATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAAt                                                                        <  1:244537/93‑1 (MQ=255)
                  aTCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATg                                                                       <  1:1040013/93‑1 (MQ=255)
                  aTCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATg                                                                       >  2:5030054/1‑93 (MQ=255)
                    cGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGcc                                                                     >  2:2919635/1‑93 (MQ=255)
                     gCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGccc                                                                    >  1:2494058/1‑93 (MQ=255)
                      ccAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCa                                                                   <  2:5841456/93‑1 (MQ=255)
                       cagcagTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAg                                                                  <  2:4764238/93‑1 (MQ=255)
                       cagcagTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAg                                                                  <  1:4982596/93‑1 (MQ=255)
                       cagcagTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAg                                                                  <  2:5462469/93‑1 (MQ=255)
                        agcagTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAgg                                                                 >  1:1129898/1‑93 (MQ=255)
                         gcagTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAggg                                                                <  1:4667041/93‑1 (MQ=255)
                         gcagTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAggg                                                                >  1:6425346/1‑93 (MQ=255)
                         gcagTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAggg                                                                >  1:1357618/1‑93 (MQ=255)
                           agTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGggcg                                                              <  2:1874334/93‑1 (MQ=255)
                           agTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGggcg                                                              >  1:5892098/1‑93 (MQ=255)
                            gTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGggcgg                                                             >  1:2201289/1‑93 (MQ=255)
                             ttGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGggcggc                                                            <  1:6123970/93‑1 (MQ=255)
                               gCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGgcggcgc                                                          >  1:2969472/1‑92 (MQ=255)
                               gCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGa                                                          <  2:4164485/93‑1 (MQ=255)
                               gCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGa                                                          >  1:5558147/1‑93 (MQ=255)
                                 aaTCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACt                                                        >  1:1474349/1‑93 (MQ=255)
                                 aaTCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACt                                                        >  1:1694933/1‑93 (MQ=255)
                                  aTCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTg                                                       <  1:5321828/93‑1 (MQ=255)
                                   tCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTgg                                                      >  1:1797058/1‑93 (MQ=255)
                                    cACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTggg                                                     <  1:517027/93‑1 (MQ=255)
                                    cACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTggg                                                     <  1:324403/93‑1 (MQ=255)
                                     aCGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTgggg                                                    <  1:4763654/93‑1 (MQ=255)
                                     aCGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTgggg                                                    <  2:4099890/93‑1 (MQ=255)
                                     aCGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTgggg                                                    >  2:4473225/1‑93 (MQ=255)
                                       gTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGa                                                  <  1:2872575/93‑1 (MQ=255)
                                       gTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGa                                                  >  2:3184138/1‑93 (MQ=255)
                                       gTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGa                                                  >  1:3802264/1‑93 (MQ=255)
                                        tATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGAt                                                 <  1:2352826/93‑1 (MQ=255)
                                         aTCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATc                                                >  2:4854108/1‑93 (MQ=255)
                                          tCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCt                                               >  1:3852908/1‑93 (MQ=255)
                                             ttCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCtgtg                                            >  2:1440118/1‑93 (MQ=255)
                                              tCATTGTGGCTTTGGACCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGa                                           <  2:2463219/93‑1 (MQ=255)
                                                 ttttGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAAt                                        <  2:5711072/93‑1 (MQ=255)
                                                 ttttGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAAt                                        <  2:5782806/93‑1 (MQ=255)
                                                 ttttGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAAt                                        <  2:6365912/93‑1 (MQ=255)
                                                  tttGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATc                                       >  2:3009425/1‑93 (MQ=255)
                                                   ttGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCa                                      <  1:1926684/93‑1 (MQ=255)
                                                   ttGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCa                                      <  2:1967078/93‑1 (MQ=255)
                                                   ttGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCa                                      <  2:2672661/93‑1 (MQ=255)
                                                    tGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAg                                     <  1:2719371/93‑1 (MQ=255)
                                                      gCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCt                                   >  1:4636896/1‑93 (MQ=255)
                                                      gCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCt                                   <  2:563946/93‑1 (MQ=255)
                                                       cTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCtt                                  >  2:1006713/1‑93 (MQ=255)
                                                       cTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCtt                                  <  1:2628193/93‑1 (MQ=255)
                                                         ttGGAGCCTTTGTCCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGGCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGt                                <  2:1623012/93‑1 (MQ=255)
                                                            gAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCa                             >  1:2151868/1‑93 (MQ=255)
                                                              gCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAgg                           >  2:5387048/1‑92 (MQ=255)
                                                              gCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGc                           >  2:37507/1‑93 (MQ=255)
                                                              gCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTCGCTCAGc                           >  2:4640082/1‑93 (MQ=255)
                                                                cTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCgg                         <  1:4210531/93‑1 (MQ=255)
                                                                 tttCACCGCGCTCATCAGGCGTTACTGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGt                        <  2:6463484/93‑1 (MQ=255)
                                                                 tttCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGt                        >  1:2744251/1‑93 (MQ=255)
                                                                   tCACCGCGCTGATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGa                      <  1:1009363/93‑1 (MQ=255)
                                                                   tCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGa                      <  1:1529837/93‑1 (MQ=255)
                                                                   tCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGa                      <  1:3618601/93‑1 (MQ=255)
                                                                    cACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGAt                     <  2:1551561/93‑1 (MQ=255)
                                                                    cACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGAt                     <  1:1680478/93‑1 (MQ=255)
                                                                      ccGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCa                   <  1:2168754/93‑1 (MQ=255)
                                                                        gcgcTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAAc                 <  2:3617003/93‑1 (MQ=255)
                                                                         cgcTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACt                <  2:3396376/93‑1 (MQ=255)
                                                                         cgcTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACt                <  1:3384701/93‑1 (MQ=255)
                                                                          gcTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACtg               <  1:5936448/93‑1 (MQ=255)
                                                                          gcTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACtg               <  1:842648/93‑1 (MQ=255)
                                                                           cTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACtga              <  2:554657/93‑1 (MQ=255)
                                                                            tcatcaGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGAGTGGGGGATCTGGGAAATAAGCTCGTTCAGCGGCGATCAACtggg             >  2:1345186/1‑91 (MQ=255)
                                                                            tcatcaGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACtgat             <  1:4392365/93‑1 (MQ=255)
                                                                              atcaGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACtgatga           >  2:2502281/1‑93 (MQ=255)
                                                                               tcaGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAg          >  2:5121369/1‑93 (MQ=255)
                                                                               tcaGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAg          >  2:4362375/1‑93 (MQ=255)
                                                                               tcaGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGGGATCAACTGATGAg          >  2:806199/1‑93 (MQ=255)
                                                                                caGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGc         <  2:175139/93‑1 (MQ=255)
                                                                                  ggCGTTACGGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCa       <  2:1224002/93‑1 (MQ=255)
                                                                                  ggCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCa       >  1:5860584/1‑93 (MQ=255)
                                                                                  ggCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCa       >  2:3488113/1‑93 (MQ=255)
                                                                                   gCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAg      <  1:4281472/93‑1 (MQ=255)
                                                                                    cGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGc     <  1:765779/93‑1 (MQ=255)
                                                                                      ttACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCTc   <  2:6390793/93‑1 (MQ=255)
                                                                                          |                                                                                         
TACACGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACTGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCTCC  >  NC_000913/2264302‑2264481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGTACACGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACTGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCTCCGCG  >  NC_000913/2264299‑2264484
                                                                                             |                                                                                            
ATGTACACGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACC                                                                                              <  SRR4302537.5850482/94‑1 (MQ=60)
ATGTACACGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACC                                                                                              <  SRR4302537.5134517/94‑1 (MQ=60)
ATGTACACGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACC                                                                                              <  SRR4302537.6164173/94‑1 (MQ=60)
  GTACACGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGA                                                                                            >  SRR4302537.5564102/1‑94 (MQ=60)
   TACACGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGAC                                                                                           >  SRR4302537.5901157/1‑94 (MQ=60)
    ACACGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACC                                                                                          <  SRR4302537.1940205/94‑1 (MQ=60)
       CGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGAT                                                                                       >  SRR4302537.936753/1‑94 (MQ=60)
       CGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGAT                                                                                       >  SRR4302537.5505950/1‑94 (MQ=60)
           CCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTG                                                                                   >  SRR4302537.3975778/1‑94 (MQ=60)
            CACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGT                                                                                  >  SRR4302537.5970968/1‑94 (MQ=60)
            CACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGT                                                                                  >  SRR4302537.3513588/1‑94 (MQ=60)
             ACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGATTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGAG                                                                                 >  SRR4302537.2179191/1‑94 (MQ=60)
              CGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACTGACCGATCGTGTGT                                                                                >  SRR4302537.1442927/1‑94 (MQ=60)
              CGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGC                                                                                >  SRR4302537.2212117/1‑94 (MQ=60)
               GCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCT                                                                               <  SRR4302537.5180969/94‑1 (MQ=60)
               GCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCT                                                                               >  SRR4302537.4890699/1‑94 (MQ=60)
               GCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCT                                                                               <  SRR4302537.1420232/94‑1 (MQ=60)
               GCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCT                                                                               >  SRR4302537.179508/1‑94 (MQ=60)
               GCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCT                                                                               <  SRR4302537.1873673/94‑1 (MQ=60)
                CTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTG                                                                              >  SRR4302537.3550235/1‑94 (MQ=60)
                CTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTG                                                                              <  SRR4302537.3823073/94‑1 (MQ=60)
                CTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTG                                                                              <  SRR4302537.611767/94‑1 (MQ=60)
                 TATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGA                                                                             >  SRR4302537.4318864/1‑94 (MQ=60)
                  ATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAA                                                                            <  SRR4302537.1787415/94‑1 (MQ=60)
                  ATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAA                                                                            >  SRR4302537.3783214/1‑94 (MQ=60)
                   TGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAAT                                                                           <  SRR4302537.510163/94‑1 (MQ=60)
                   TGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAAT                                                                           <  SRR4302537.1413369/94‑1 (MQ=60)
                    GATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGGTGAATG                                                                          >  SRR4302537.282661/1‑94 (MQ=60)
                    GATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATG                                                                          <  SRR4302537.246268/94‑1 (MQ=60)
                    GATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATG                                                                          <  SRR4302537.1469800/94‑1 (MQ=60)
                     ATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGC                                                                         <  SRR4302537.1047171/94‑1 (MQ=60)
                       CGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCC                                                                       >  SRR4302537.2511618/1‑94 (MQ=60)
                        GCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCA                                                                      >  SRR4302537.1214775/1‑94 (MQ=60)
                        GCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCA                                                                      <  SRR4302537.2971884/94‑1 (MQ=60)
                          CAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGG                                                                    <  SRR4302537.5469297/94‑1 (MQ=60)
                          CAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGG                                                                    <  SRR4302537.5016938/94‑1 (MQ=60)
                          CAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGG                                                                    >  SRR4302537.1137738/1‑94 (MQ=60)
                           AGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGG                                                                   >  SRR4302537.6469007/1‑94 (MQ=60)
                           AGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGG                                                                   >  SRR4302537.1367072/1‑94 (MQ=60)
                            GCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGC                                                                  <  SRR4302537.4699328/94‑1 (MQ=60)
                             CAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCG                                                                 >  SRR4302537.3698569/1‑94 (MQ=60)
                             CAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCG                                                                 >  SRR4302537.5932527/1‑94 (MQ=60)
                             CAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCG                                                                 <  SRR4302537.5056372/94‑1 (MQ=60)
                              AGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGG                                                                >  SRR4302537.2216805/1‑94 (MQ=60)
                              AGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGG                                                                >  SRR4302537.405931/1‑94 (MQ=60)
                              AGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGG                                                                >  SRR4302537.6165202/1‑94 (MQ=60)
                              AGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGG                                                                <  SRR4302537.1430289/94‑1 (MQ=60)
                                TTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCG                                                              >  SRR4302537.4680641/1‑94 (MQ=60)
                                TTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCG                                                              <  SRR4302537.6166066/94‑1 (MQ=60)
                                 TGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGC                                                             >  SRR4302537.2990290/1‑94 (MQ=60)
                                 TGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGA                                                             >  SRR4302537.5596122/1‑94 (MQ=60)
                                 TGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGA                                                             >  SRR4302537.1437835/1‑94 (MQ=60)
                                   CAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACT                                                           <  SRR4302537.5396413/94‑1 (MQ=60)
                                   CAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACT                                                           >  SRR4302537.1484595/1‑94 (MQ=60)
                                   CAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACT                                                           >  SRR4302537.1706791/1‑94 (MQ=60)
                                   CAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACT                                                           >  SRR4302537.5761925/1‑94 (MQ=60)
                                    AATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTG                                                          >  SRR4302537.6055987/1‑94 (MQ=60)
                                     ATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGG                                                         >  SRR4302537.1809602/1‑94 (MQ=60)
                                     ATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGG                                                         <  SRR4302537.5358353/94‑1 (MQ=60)
                                       CACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGG                                                       <  SRR4302537.326652/94‑1 (MQ=60)
                                       CACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGG                                                       <  SRR4302537.1877242/94‑1 (MQ=60)
                                       CACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGG                                                       >  SRR4302537.3500814/1‑94 (MQ=60)
                                       CACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGG                                                       <  SRR4302537.520583/94‑1 (MQ=60)
                                        ACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGG                                                      >  SRR4302537.4657221/1‑94 (MQ=60)
                                        ACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGG                                                      <  SRR4302537.4796554/94‑1 (MQ=60)
                                         CGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGA                                                     >  SRR4302537.5678489/1‑94 (MQ=60)
                                         CGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGA                                                     >  SRR4302537.3828935/1‑94 (MQ=60)
                                          GTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGAT                                                    <  SRR4302537.2892733/94‑1 (MQ=60)
                                           TATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATC                                                   <  SRR4302537.2369414/94‑1 (MQ=60)
                                            ATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCT                                                  >  SRR4302537.700751/1‑94 (MQ=60)
                                            ATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCT                                                  >  SRR4302537.3879884/1‑94 (MQ=60)
                                             TCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTG                                                 >  SRR4302537.3335288/1‑94 (MQ=60)
                                              CGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGT                                                <  SRR4302537.4414196/94‑1 (MQ=60)
                                               GTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTG                                               >  SRR4302537.3550595/1‑94 (MQ=60)
                                                TTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGA                                              >  SRR4302537.2147041/1‑94 (MQ=60)
                                                  CATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAA                                            >  SRR4302537.3955397/1‑94 (MQ=60)
                                                   ATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGGCCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAAT                                           <  SRR4302537.4735976/94‑1 (MQ=60)
                                                   ATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAAT                                           >  SRR4302537.2681268/1‑94 (MQ=60)
                                                      TTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAG                                        <  SRR4302537.1940055/94‑1 (MQ=60)
                                                       TGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGCTCGTGGGCTGAATTCCCAGGGCGGCGACTGAGGGGTCTGTCAAATCAGT                                       >  SRR4302537.2158494/1‑94 (MQ=60)
                                                       TGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGC                                       <  SRR4302537.2738364/94‑1 (MQ=60)
                                                        GGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCT                                      <  SRR4302537.3820251/94‑1 (MQ=60)
                                                        GGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCT                                      >  SRR4302537.4668969/1‑94 (MQ=60)
                                                        GGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCT                                      >  SRR4302537.3858468/1‑94 (MQ=60)
                                                         GCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTT                                     <  SRR4302537.30285/94‑1 (MQ=60)
                                                          CTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTG                                    <  SRR4302537.2646602/94‑1 (MQ=60)
                                                          CTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTG                                    >  SRR4302537.1553148/1‑94 (MQ=60)
                                                            TTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTT                                  <  SRR4302537.5062509/94‑1 (MQ=60)
                                                            TTGGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTT                                  >  SRR4302537.4757701/1‑94 (MQ=60)
                                                              GGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCA                                <  SRR4302537.2720377/94‑1 (MQ=60)
                                                              GGAGCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCA                                >  SRR4302537.2167035/1‑94 (MQ=60)
                                                                 GCCTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCG                             <  SRR4302537.1071658/94‑1 (MQ=60)
                                                                   CTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGT                           <  SRR4302537.4254015/94‑1 (MQ=60)
                                                                   CTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGT                           <  SRR4302537.4239900/94‑1 (MQ=60)
                                                                   CTTTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGT                           >  SRR4302537.2763395/1‑94 (MQ=60)
                                                                     TTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGA                         >  SRR4302537.1021546/1‑94 (MQ=60)
                                                                     TTCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGA                         <  SRR4302537.2190650/94‑1 (MQ=60)
                                                                      TCACCGCGCTGATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGAT                        <  SRR4302537.1016324/94‑1 (MQ=60)
                                                                      TCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGAT                        >  SRR4302537.3278839/1‑94 (MQ=60)
                                                                      TCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGAT                        <  SRR4302537.1540527/94‑1 (MQ=60)
                                                                      TCACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGAT                        <  SRR4302537.3644108/94‑1 (MQ=60)
                                                                       CACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATC                       <  SRR4302537.1692240/94‑1 (MQ=60)
                                                                       CACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATC                       <  SRR4302537.2477295/94‑1 (MQ=60)
                                                                        CCCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCA                      <  SRR4302537.6042797/94‑1 (MQ=60)
                                                                        ACCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCA                      >  SRR4302537.1816458/1‑94 (MQ=60)
                                                                         CCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAA                     <  SRR4302537.2184032/94‑1 (MQ=60)
                                                                         CCGCGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAA                     >  SRR4302537.2738879/1‑94 (MQ=60)
                                                                            CGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTG                  <  SRR4302537.3408680/94‑1 (MQ=60)
                                                                            CGCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTG                  >  SRR4302537.298773/1‑94 (MQ=60)
                                                                             GCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGA                 <  SRR4302537.848423/94‑1 (MQ=60)
                                                                             GCTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGA                 <  SRR4302537.5977190/94‑1 (MQ=60)
                                                                              CTCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGAT                <  SRR4302537.3534638/94‑1 (MQ=60)
                                                                               TCATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATG               <  SRR4302537.4422672/94‑1 (MQ=60)
                                                                                CATCAGGCGTTACTGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGA              >  SRR4302537.2448361/1‑94 (MQ=60)
                                                                                 ATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAG             <  SRR4302537.2545360/94‑1 (MQ=60)
                                                                                 ATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAG             >  SRR4302537.2465406/1‑94 (MQ=60)
                                                                                 ATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAG             <  SRR4302537.1515341/94‑1 (MQ=60)
                                                                                 ATCAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAG             >  SRR4302537.1159665/1‑94 (MQ=60)
                                                                                   CAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCC           >  SRR4302537.1700852/1‑94 (MQ=60)
                                                                                   CAGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCC           <  SRR4302537.1614428/94‑1 (MQ=60)
                                                                                    AGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCA          >  SRR4302537.5900771/1‑94 (MQ=60)
                                                                                    AGGCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTATTCAGCGGTGATCAACTGATGAGCCA          >  SRR4302537.838811/1‑94 (MQ=60)
                                                                                     GGCGTTACTGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAG         <  SRR4302537.526693/94‑1 (MQ=60)
                                                                                      GCGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGC        <  SRR4302537.4311236/94‑1 (MQ=60)
                                                                                       CGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCT       >  SRR4302537.2257899/1‑94 (MQ=60)
                                                                                       CGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCT       >  SRR4302537.4119641/1‑94 (MQ=60)
                                                                                       CGTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCT       <  SRR4302537.771024/94‑1 (MQ=60)
                                                                                        GTTACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCTC      >  SRR4302537.6380098/1‑94 (MQ=60)
                                                                                          TACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCTCCG    <  SRR4302537.3637021/94‑1 (MQ=60)
                                                                                          TACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCTCCG    <  SRR4302537.6283963/94‑1 (MQ=60)
                                                                                           ACCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCTCCGC   <  SRR4302537.5315317/94‑1 (MQ=60)
                                                                                            CCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCTCCGCG  <  SRR4302537.4996339/94‑1 (MQ=60)
                                                                                            CCGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCTCCGCG  <  SRR4302537.4133156/94‑1 (MQ=60)
                                                                                            CCGACCGATCGTGTGCTGAATGCACAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCTCCGCG  <  SRR4302537.5019092/94‑1 (MQ=60)
                                                                                             |                                                                                            
ATGTACACGCTCCACGCTATGATCGCCAGCAGTTGCAATCACGTATCGTTCATTTTGGCTTTGGAGCCTTTCACCGCGCTCATCAGGCGTTACTGACCGATCGTGTGCTGAATGCCCAGGGCGGCGACTGGGGGATCTGTGAAATCAGCTTGTTCAGCGGTGATCAACTGATGAGCCAGCTCCGCG  >  NC_000913/2264299‑2264484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑