Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,226 T→C V55A (GTC→GCC)  atoS → AtoS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,2260TC100.0% 793.8 / NA 230V55A (GTC→GCC) atoSAtoS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (121/109);  total (121/109)

Only 100 of 233 total aligned reads displayed.

TTGTCCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAAC  >  NC_000913/2318136‑2318315
                                                                                          |                                                                                         
ttGTCCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCa                                                                                         <  2:1432551/93‑1 (MQ=255)
   tCCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAAcc                                                                                      >  2:3956100/1‑93 (MQ=255)
    cccAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCt                                                                                     <  1:5175515/93‑1 (MQ=255)
     ccAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTg                                                                                    >  1:6013857/1‑93 (MQ=255)
     ccAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTg                                                                                    >  2:2110991/1‑93 (MQ=255)
       aaCGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCt                                                                                  >  2:2976379/1‑93 (MQ=255)
       aaCGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCt                                                                                  >  2:4892423/1‑93 (MQ=255)
          gCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTaa                                                                               >  1:347373/1‑93 (MQ=255)
          gCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTaa                                                                               >  2:1647479/1‑93 (MQ=255)
            ttACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATc                                                                             >  1:1023181/1‑93 (MQ=255)
              aCTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAg                                                                           >  1:5850057/1‑93 (MQ=255)
              aCTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAg                                                                           <  2:127591/93‑1 (MQ=255)
               cTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACTTGCTTAATCAgg                                                                          <  2:5402557/93‑1 (MQ=255)
                tATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGc                                                                         <  2:3749135/93‑1 (MQ=255)
                  ttGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCAc                                                                       <  1:508717/93‑1 (MQ=255)
                  ttGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCAc                                                                       <  1:3117915/93‑1 (MQ=255)
                  ttGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCAc                                                                       >  2:2419000/1‑93 (MQ=255)
                    ggTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTa                                                                     <  1:3779713/93‑1 (MQ=255)
                     gTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAg                                                                    <  2:2561301/93‑1 (MQ=255)
                      ttATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAgg                                                                   <  2:3155235/93‑1 (MQ=255)
                        atatCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCg                                                                 <  1:2900422/93‑1 (MQ=255)
                         tatCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGa                                                                <  1:3306079/93‑1 (MQ=255)
                         tatCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGa                                                                >  1:1866197/1‑93 (MQ=255)
                         tatCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGa                                                                <  1:4144153/93‑1 (MQ=255)
                          atCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGAt                                                               <  2:5763908/93‑1 (MQ=255)
                           tCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATc                                                              >  1:1341319/1‑93 (MQ=255)
                           tCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACCAGGCGATc                                                              >  2:871516/1‑93 (MQ=255)
                             gTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGc                                                            >  2:3720749/1‑93 (MQ=255)
                                gAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTAt                                                         >  2:4774131/1‑93 (MQ=255)
                                gAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTAt                                                         >  1:236718/1‑93 (MQ=255)
                                    cGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtc                                                     <  2:4473123/93‑1 (MQ=255)
                                     ggAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtct                                                    <  1:6416000/93‑1 (MQ=255)
                                     ggAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtct                                                    <  2:6341466/93‑1 (MQ=255)
                                      gAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtctc                                                   <  2:4369063/93‑1 (MQ=255)
                                      gAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtctc                                                   >  2:4859556/1‑93 (MQ=255)
                                      gAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtctc                                                   >  1:2290538/1‑93 (MQ=255)
                                        aGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTa                                                 >  2:3651961/1‑93 (MQ=255)
                                          gACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACa                                               <  1:3192809/93‑1 (MQ=255)
                                           aCGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACAt                                              >  2:4026818/1‑93 (MQ=255)
                                           aCGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACAt                                              >  2:5839065/1‑93 (MQ=255)
                                            cGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATc                                             >  2:1117450/1‑93 (MQ=255)
                                             gTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCg                                            >  1:4942212/1‑93 (MQ=255)
                                              ttCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCgc                                           >  1:3561548/1‑92 (MQ=255)
                                              ttCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGa                                           <  2:3300148/93‑1 (MQ=255)
                                              ttCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGa                                           <  1:5709217/93‑1 (MQ=255)
                                              ttCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGa                                           <  2:1727088/93‑1 (MQ=255)
                                              ttCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGa                                           >  1:276525/1‑93 (MQ=255)
                                               tCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGAc                                          >  2:3903967/1‑93 (MQ=255)
                                               tCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGAc                                          >  1:6130809/1‑93 (MQ=255)
                                               tCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGAc                                          <  2:3422953/93‑1 (MQ=255)
                                                 agcagTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACtt                                        >  1:4873342/1‑93 (MQ=255)
                                                 agcagTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACtt                                        <  2:1876618/93‑1 (MQ=255)
                                                 agcagTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACtt                                        >  2:2056572/1‑93 (MQ=255)
                                                 agcagTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACtt                                        >  2:6050493/1‑93 (MQ=255)
                                                  gcagTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTa                                       >  1:811029/1‑93 (MQ=255)
                                                  gcagTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTa                                       >  1:5513335/1‑93 (MQ=255)
                                                   cagTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTAc                                      >  2:92539/1‑93 (MQ=255)
                                                    agTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTAcc                                     <  1:4025711/93‑1 (MQ=255)
                                                     gTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCa                                    <  2:5197712/93‑1 (MQ=255)
                                                     gTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCa                                    <  2:5322386/93‑1 (MQ=255)
                                                     gTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCa                                    >  2:5481659/1‑93 (MQ=255)
                                                      tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCAc                                   >  1:4915033/1‑93 (MQ=255)
                                                      tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCAc                                   <  2:2748644/93‑1 (MQ=255)
                                                       cTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACg                                  <  1:937926/93‑1 (MQ=255)
                                                        ttATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGt                                 >  1:3660892/1‑93 (MQ=255)
                                                        ttATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGt                                 <  1:1606399/93‑1 (MQ=255)
                                                         tATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTg                                >  1:155981/1‑93 (MQ=255)
                                                          aTCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTga                               >  2:5607424/1‑93 (MQ=255)
                                                          aTCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTga                               >  2:693375/1‑93 (MQ=255)
                                                          aTCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTga                               <  2:4187751/93‑1 (MQ=255)
                                                           tCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTgag                              >  2:6243420/1‑93 (MQ=255)
                                                           tCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTgag                              >  1:2615039/1‑93 (MQ=255)
                                                             tGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTgagga                            >  1:1286473/1‑93 (MQ=255)
                                                              gAAAAAGAGAAAAAACTTTCTGCCGTGGCCAAGCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTgaggag                           <  1:3279405/93‑1 (MQ=255)
                                                              gAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTgaggag                           >  2:5067265/1‑93 (MQ=255)
                                                              gAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTgaggag                           <  2:154740/93‑1 (MQ=255)
                                                              gAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTgaggag                           >  1:391369/1‑93 (MQ=255)
                                                                aaaaGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCg                         <  2:2354805/93‑1 (MQ=255)
                                                                aaaaGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCg                         >  2:3219393/1‑93 (MQ=255)
                                                                 aaaGAGAAAAAACTTTCTGCTGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGt                        >  1:4603946/1‑93 (MQ=255)
                                                                  aaGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa                       <  2:5921090/93‑1 (MQ=255)
                                                                   agagAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTAt                      >  2:3226995/1‑93 (MQ=255)
                                                                     agaAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATcc                    >  1:4538794/1‑93 (MQ=255)
                                                                       aaaaaaCTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCcgc                  >  1:6306673/1‑93 (MQ=255)
                                                                       aaaaaaCTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCcgc                  <  2:1363634/93‑1 (MQ=255)
                                                                        aaaaaCTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCcgcg                 <  1:1711778/93‑1 (MQ=255)
                                                                            aCTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCAtt             <  1:2677976/93‑1 (MQ=255)
                                                                               ttCTGCCGGGGCCAACCTGCTTAATCAGGCCCTAGGCGCTCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTaaa          <  1:4214775/93‑1 (MQ=255)
                                                                               tgcTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTaaa          <  1:4591188/91‑1 (MQ=255)
                                                                                tCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAAt         >  1:5328803/1‑93 (MQ=255)
                                                                                tCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAAt         <  2:3187203/93‑1 (MQ=255)
                                                                                   gCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCa      <  2:283100/93‑1 (MQ=255)
                                                                                   gCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCa      <  1:303059/93‑1 (MQ=255)
                                                                                   gCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCa      <  1:3882724/93‑1 (MQ=255)
                                                                                   gCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCa      >  1:5463234/1‑93 (MQ=255)
                                                                                     cGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGa    >  1:1990779/1‑93 (MQ=255)
                                                                                     cGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGa    <  1:1159370/93‑1 (MQ=255)
                                                                                      gTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGaa   >  1:5412057/1‑93 (MQ=255)
                                                                                       tGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAAc  >  1:2432648/1‑93 (MQ=255)
                                                                                       tGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTCAGCAGCGTCTCCGCGCATTAAATGCAGAAc  >  1:303637/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTGTCCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAAC  >  NC_000913/2318136‑2318315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATTGTCCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTTGC  >  NC_000913/2318134‑2318319
                                                                                            |                                                                                             
CATTGTCCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCC                                                                                              >  SRR4302537.6167940/1‑94 (MQ=60)
CATTGTCCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCC                                                                                              >  SRR4302537.4899940/1‑94 (MQ=60)
CATTGTCCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCC                                                                                              >  SRR4302537.1557447/1‑94 (MQ=60)
 ATTGTCCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCA                                                                                             <  SRR4302537.2298981/94‑1 (MQ=60)
      CCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTG                                                                                        >  SRR4302537.3182093/1‑94 (MQ=60)
      CCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTG                                                                                        >  SRR4302537.6055174/1‑94 (MQ=60)
      CCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTG                                                                                        >  SRR4302537.5063692/1‑94 (MQ=60)
      CCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTG                                                                                        <  SRR4302537.5211138/94‑1 (MQ=60)
       CCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGC                                                                                       >  SRR4302537.4156574/1‑94 (MQ=60)
           CGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAA                                                                                   >  SRR4302537.349787/1‑94 (MQ=60)
             CTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATC                                                                                 >  SRR4302537.1030223/1‑94 (MQ=60)
             CCTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATC                                                                                 >  SRR4302537.3411768/1‑94 (MQ=60)
               TACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAG                                                                               >  SRR4302537.1437594/1‑94 (MQ=60)
               TACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAG                                                                               >  SRR4302537.5890184/1‑94 (MQ=60)
                ACTATTGGTTATATCGTAGAAACGGAAGGATGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGG                                                                              >  SRR4302537.906329/1‑94 (MQ=60)
                ACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGG                                                                              <  SRR4302537.1520649/94‑1 (MQ=60)
                ACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGG                                                                              <  SRR4302537.5405886/94‑1 (MQ=60)
                ACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGG                                                                              <  SRR4302537.6327823/94‑1 (MQ=60)
                  TATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCA                                                                            <  SRR4302537.4174882/94‑1 (MQ=60)
                  TATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCA                                                                            >  SRR4302537.6098333/1‑94 (MQ=60)
                    TTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACT                                                                          <  SRR4302537.512211/94‑1 (MQ=60)
                    TTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACT                                                                          <  SRR4302537.1672027/94‑1 (MQ=60)
                    TTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACT                                                                          >  SRR4302537.4368136/1‑94 (MQ=60)
                    TTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACT                                                                          <  SRR4302537.3139696/94‑1 (MQ=60)
                     TGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTA                                                                         <  SRR4302537.4044430/94‑1 (MQ=60)
                     TGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTA                                                                         <  SRR4302537.993293/94‑1 (MQ=60)
                      GGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAG                                                                        <  SRR4302537.3806258/94‑1 (MQ=60)
                       GTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGCAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGG                                                                       <  SRR4302537.4582331/94‑1 (MQ=60)
                       GTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGG                                                                       <  SRR4302537.381040/94‑1 (MQ=60)
                        TTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGC                                                                      <  SRR4302537.312667/94‑1 (MQ=60)
                         TATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCG                                                                     <  SRR4302537.2549110/94‑1 (MQ=60)
                         TATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCG                                                                     <  SRR4302537.2808460/94‑1 (MQ=60)
                          ATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGA                                                                    >  SRR4302537.191125/1‑94 (MQ=60)
                          ATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGA                                                                    >  SRR4302537.1879191/1‑94 (MQ=60)
                          ATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGA                                                                    <  SRR4302537.2920799/94‑1 (MQ=60)
                           TATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGAT                                                                   <  SRR4302537.3329450/94‑1 (MQ=60)
                           TATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGAT                                                                   <  SRR4302537.4173063/94‑1 (MQ=60)
                            ATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATC                                                                  <  SRR4302537.2337679/94‑1 (MQ=60)
                            ATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATC                                                                  >  SRR4302537.1350659/1‑94 (MQ=60)
                             TCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCG                                                                 <  SRR4302537.6161015/94‑1 (MQ=60)
                                 AGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTAT                                                             >  SRR4302537.241866/1‑94 (MQ=60)
                                 AGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTAT                                                             >  SRR4302537.238386/1‑94 (MQ=60)
                                    AACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAT                                                          <  SRR4302537.2045906/94‑1 (MQ=60)
                                      CGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGTAACTAGGCGATCGCTATGATCT                                                        >  SRR4302537.781707/1‑94 (MQ=60)
                                      CGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCT                                                        >  SRR4302537.5580211/1‑94 (MQ=60)
                                       GGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTC                                                       >  SRR4302537.2306712/1‑94 (MQ=60)
                                       GGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTC                                                       <  SRR4302537.6459636/94‑1 (MQ=60)
                                         AAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTA                                                     >  SRR4302537.3055852/1‑94 (MQ=60)
                                         AAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTA                                                     >  SRR4302537.3441747/1‑94 (MQ=60)
                                          AGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTAC                                                    >  SRR4302537.4783029/1‑94 (MQ=60)
                                            GACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACAT                                                  <  SRR4302537.1600991/94‑1 (MQ=60)
                                            GACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACAT                                                  <  SRR4302537.3215258/94‑1 (MQ=60)
                                              CGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCG                                                >  SRR4302537.4666341/1‑94 (MQ=60)
                                              CGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCG                                                >  SRR4302537.4976293/1‑94 (MQ=60)
                                               GTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGC                                               >  SRR4302537.3586672/1‑94 (MQ=60)
                                               GTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGA                                               >  SRR4302537.278459/1‑94 (MQ=60)
                                               GTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGA                                               >  SRR4302537.2951511/1‑94 (MQ=60)
                                                TTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGAC                                              >  SRR4302537.6172949/1‑94 (MQ=60)
                                                TTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGAC                                              <  SRR4302537.5748285/94‑1 (MQ=60)
                                                  CAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTT                                            >  SRR4302537.4906976/1‑94 (MQ=60)
                                                   AGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTA                                           >  SRR4302537.5550982/1‑94 (MQ=60)
                                                   AGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTA                                           >  SRR4302537.816589/1‑94 (MQ=60)
                                                   AGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTA                                           <  SRR4302537.6375133/94‑1 (MQ=60)
                                                   AGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTA                                           >  SRR4302537.5282173/1‑94 (MQ=60)
                                                      AGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCA                                        <  SRR4302537.4053895/94‑1 (MQ=60)
                                                      AGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCA                                        >  SRR4302537.5461752/1‑94 (MQ=60)
                                                      AGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCA                                        <  SRR4302537.1122695/94‑1 (MQ=60)
                                                      AGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCA                                        >  SRR4302537.3022219/1‑94 (MQ=60)
                                                       GTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCAC                                       >  SRR4302537.4948953/1‑94 (MQ=60)
                                                         CTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGT                                     >  SRR4302537.3686659/1‑94 (MQ=60)
                                                         CTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGT                                     <  SRR4302537.944392/94‑1 (MQ=60)
                                                          TTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTG                                    >  SRR4302537.5544164/1‑94 (MQ=60)
                                                          TTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTG                                    <  SRR4302537.1617631/94‑1 (MQ=60)
                                                          TTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTG                                    >  SRR4302537.157091/1‑94 (MQ=60)
                                                            ATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAG                                  >  SRR4302537.2633330/1‑94 (MQ=60)
                                                            ATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAG                                  <  SRR4302537.3949010/94‑1 (MQ=60)
                                                             TCTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGG                                 >  SRR4302537.2370098/1‑94 (MQ=60)
                                                              CTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGA                                >  SRR4302537.1295420/1‑94 (MQ=60)
                                                              CTGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGA                                >  SRR4302537.5641738/1‑94 (MQ=60)
                                                               TGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAG                               >  SRR4302537.5841495/1‑94 (MQ=60)
                                                               TGAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAG                               >  SRR4302537.394076/1‑94 (MQ=60)
                                                                GAAAAAGAGAAAAAACTTTCTGCCGTGGCCAAGCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGC                              <  SRR4302537.3302554/94‑1 (MQ=60)
                                                                GAAAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGC                              <  SRR4302537.3939229/94‑1 (MQ=60)
                                                                  AAAAGAGAAAAAACTTTCTGCTGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGT                            >  SRR4302537.4635822/1‑94 (MQ=60)
                                                                  AAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGT                            >  SRR4302537.4938248/1‑94 (MQ=60)
                                                                  AAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGT                            >  SRR4302537.2653401/1‑94 (MQ=60)
                                                                  AAAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGT                            <  SRR4302537.2692758/94‑1 (MQ=60)
                                                                   AAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGCCTTACCACGTGAGGAGCGTA                           >  SRR4302537.3662216/1‑94 (MQ=60)
                                                                   AAAGAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTA                           >  SRR4302537.5077459/1‑94 (MQ=60)
                                                                      GAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCC                        <  SRR4302537.5943020/94‑1 (MQ=60)
                                                                      GAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCC                        <  SRR4302537.1206091/94‑1 (MQ=60)
                                                                      GAGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCC                        >  SRR4302537.4570214/1‑94 (MQ=60)
                                                                       AGAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCG                       >  SRR4302537.3775527/1‑94 (MQ=60)
                                                                        GAAAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGC                      >  SRR4302537.6349860/1‑94 (MQ=60)
                                                                          AAAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGC                    <  SRR4302537.1723733/94‑1 (MQ=60)
                                                                           AAAACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCA                   >  SRR4302537.4559552/1‑94 (MQ=60)
                                                                             AACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATT                 >  SRR4302537.1838931/1‑94 (MQ=60)
                                                                              ACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTA                <  SRR4302537.2696677/94‑1 (MQ=60)
                                                                              ACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTA                >  SRR4302537.4950223/1‑94 (MQ=60)
                                                                              ACTTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTA                >  SRR4302537.3150075/1‑94 (MQ=60)
                                                                                TTTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAA              <  SRR4302537.2766814/94‑1 (MQ=60)
                                                                                 TTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAAT             >  SRR4302537.5365362/1‑94 (MQ=60)
                                                                                 TTCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAAT             <  SRR4302537.6488989/94‑1 (MQ=60)
                                                                                 TTCTGCCGGGGCCAACCTGCTTAATCAGGCCCTAGGCGCTCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAAT             <  SRR4302537.4244162/94‑1 (MQ=60)
                                                                                 TGCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAAT             <  SRR4302537.4622946/94‑1 (MQ=60)
                                                                                  TCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATG            <  SRR4302537.1338821/94‑1 (MQ=60)
                                                                                  TCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATG            <  SRR4302537.1735053/94‑1 (MQ=60)
                                                                                  TCTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATG            <  SRR4302537.5411632/94‑1 (MQ=60)
                                                                                   CTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGC           <  SRR4302537.5157034/94‑1 (MQ=60)
                                                                                   CTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGC           >  SRR4302537.3519734/1‑94 (MQ=60)
                                                                                   CTGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGC           >  SRR4302537.3268424/1‑94 (MQ=60)
                                                                                    TGCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCA          >  SRR4302537.5500499/1‑94 (MQ=60)
                                                                                     GCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAG         <  SRR4302537.3909885/94‑1 (MQ=60)
                                                                                     GCCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAG         <  SRR4302537.305168/94‑1 (MQ=60)
                                                                                      CCGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGA        >  SRR4302537.2004616/1‑94 (MQ=60)
                                                                                       CGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAA       >  SRR4302537.5448960/1‑94 (MQ=60)
                                                                                       CGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAA       <  SRR4302537.1167435/94‑1 (MQ=60)
                                                                                       CGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAA       <  SRR4302537.36962/94‑1 (MQ=60)
                                                                                       CGTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAA       <  SRR4302537.4628122/94‑1 (MQ=60)
                                                                                        GTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAAC      >  SRR4302537.2449785/1‑94 (MQ=60)
                                                                                        GTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAAC      <  SRR4302537.3316055/94‑1 (MQ=60)
                                                                                        GTGGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTCAGCAGCGTCTCCGCGCATTAAATGCAGAAC      >  SRR4302537.305750/1‑94 (MQ=60)
                                                                                          GGCCAACCTGGTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTT    <  SRR4302537.4842749/94‑1 (MQ=60)
                                                                                          GGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTT    >  SRR4302537.241537/1‑94 (MQ=60)
                                                                                          GGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTT    <  SRR4302537.2334195/94‑1 (MQ=60)
                                                                                          GGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTT    >  SRR4302537.1286474/1‑94 (MQ=60)
                                                                                          GGCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTT    <  SRR4302537.453388/94‑1 (MQ=60)
                                                                                           GCCAACCTGCTTAATCAGGCACTAGGCGATTGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTTG   <  SRR4302537.2125871/94‑1 (MQ=60)
                                                                                           GCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTTG   <  SRR4302537.5922589/94‑1 (MQ=60)
                                                                                           GCCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTTG   <  SRR4302537.2271321/94‑1 (MQ=60)
                                                                                            CCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTTGC  >  SRR4302537.1997053/1‑94 (MQ=60)
                                                                                            CCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTTGC  <  SRR4302537.5416248/94‑1 (MQ=60)
                                                                                            CCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTTGC  >  SRR4302537.4288994/1‑94 (MQ=60)
                                                                                            CCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTTGC  >  SRR4302537.4442563/1‑94 (MQ=60)
                                                                                            CCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTTGC  >  SRR4302537.1014455/1‑94 (MQ=60)
                                                                                            |                                                                                             
CATTGTCCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGTTCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACTTGC  >  NC_000913/2318134‑2318319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑