Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,332,579 A→G L134P (CTG→CCG)  b2229 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,332,5790AG98.8% 855.9 / ‑2.0 253L134P (CTG→CCG) b2229hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (122/128);  total (122/131)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 263 total aligned reads displayed.

GGCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCAGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCCCT  >  NC_000913/2332488‑2332669
                                                                                           |                                                                                          
ggCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAggcggc                                                                                          <  1:7401736/94‑1 (MQ=255)
ggCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAggcggc                                                                                          >  1:6061113/1‑94 (MQ=255)
  cgcgTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGTCATCGCCCTGATCGAATAAAATCATATCGCCAGGCGgcgc                                                                                        <  2:5096266/94‑1 (MQ=255)
    cTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCt                                                                                      >  1:258316/1‑94 (MQ=255)
    cTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCt                                                                                      >  2:2505375/1‑94 (MQ=255)
    cTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCt                                                                                      <  2:8972464/94‑1 (MQ=255)
         ggTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGttt                                                                                 >  1:1587217/1‑94 (MQ=255)
         ggTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGttt                                                                                 >  1:4395347/1‑94 (MQ=255)
           tgtgGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTAt                                                                               <  2:7022985/94‑1 (MQ=255)
               gTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTcc                                                                           >  2:7519334/1‑94 (MQ=255)
                gaGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCt                                                                          <  2:1456646/93‑1 (MQ=255)
                 aGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTg                                                                         >  2:6919971/1‑94 (MQ=255)
                 aGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTg                                                                         <  1:4684167/94‑1 (MQ=255)
                 aGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTg                                                                         >  2:7323969/1‑94 (MQ=255)
                  aaggACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTgg                                                                        <  1:7811023/91‑1 (MQ=255)
                     gACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCa                                                                     <  2:6223457/94‑1 (MQ=255)
                       cGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAAt                                                                   >  1:425871/1‑94 (MQ=255)
                       cGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGACAGGCGGCGCCTGGTTTATGTCCTGGCCAAt                                                                   >  1:9068855/1‑94 (MQ=255)
                       cGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCATGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAAt                                                                   <  2:5162855/94‑1 (MQ=255)
                        gTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATa                                                                  <  1:510734/94‑1 (MQ=255)
                         tAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTTCTTGCCAATaa                                                                 >  1:990252/1‑94 (MQ=255)
                         tAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATaa                                                                 <  2:9292067/94‑1 (MQ=255)
                             gCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTg                                                             <  2:341797/94‑1 (MQ=255)
                             gCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTg                                                             <  1:10371206/94‑1 (MQ=255)
                             gCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGATTATGTCCTGGCCAATAAACTg                                                             >  1:5653666/1‑94 (MQ=255)
                              ccccATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTgg                                                            <  2:2258227/94‑1 (MQ=255)
                              ccccATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTgg                                                            >  2:4256929/1‑94 (MQ=255)
                              ccccATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTgg                                                            >  2:4483028/1‑94 (MQ=255)
                               cccATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGc                                                           >  2:3129234/1‑94 (MQ=255)
                                ccATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCt                                                          <  2:9837718/94‑1 (MQ=255)
                                 cATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCtg                                                         <  2:4860308/94‑1 (MQ=255)
                                  aTCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCtgt                                                        >  1:1905106/1‑94 (MQ=255)
                                  aTCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCtgt                                                        <  1:6535591/94‑1 (MQ=255)
                                    ccAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCtgttg                                                      <  1:3885422/94‑1 (MQ=255)
                                     cAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCtgttgt                                                     >  1:8275333/1‑94 (MQ=255)
                                      aGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTa                                                    >  2:8348131/1‑94 (MQ=255)
                                       gACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTAc                                                   <  2:5043210/94‑1 (MQ=255)
                                        aCCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACt                                                  <  2:7970024/94‑1 (MQ=255)
                                        aCCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACt                                                  <  1:1054525/94‑1 (MQ=255)
                                          cATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGTCCAATAAACTGGCTGTTGTTCTGa                                                >  1:1324892/1‑94 (MQ=255)
                                          cATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGa                                                >  2:10211759/1‑94 (MQ=255)
                                           aTTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGaa                                               >  1:4096077/1‑94 (MQ=255)
                                            ttAAGGGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAAt                                              <  2:8139902/94‑1 (MQ=255)
                                             tAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATc                                             <  1:6916708/94‑1 (MQ=255)
                                              aaGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCa                                            <  1:6719398/94‑1 (MQ=255)
                                               aGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCaa                                           >  1:6418666/1‑94 (MQ=255)
                                                  gCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCTCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAAtt                                        <  1:2962571/94‑1 (MQ=255)
                                                   cTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTa                                       <  1:9783196/94‑1 (MQ=255)
                                                    tGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGAGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTaa                                      >  1:2761651/1‑94 (MQ=255)
                                                     gggCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAAt                                     <  2:3191697/94‑1 (MQ=255)
                                                      ggCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATc                                    >  1:1552229/1‑94 (MQ=255)
                                                      ggCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATc                                    <  2:3842756/94‑1 (MQ=255)
                                                      ggCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATc                                    <  2:9708142/94‑1 (MQ=255)
                                                      ggCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATc                                    <  2:8341812/94‑1 (MQ=255)
                                                      ggCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATc                                    <  1:9190601/94‑1 (MQ=255)
                                                        cATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATcgc                                  <  2:10287115/94‑1 (MQ=255)
                                                         aTCGCCCTGATGGAAAAAAATCATATCGCCAGCCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATcgcg                                 <  2:6353073/94‑1 (MQ=255)
                                                         aTCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATcgcg                                 <  2:2825096/94‑1 (MQ=255)
                                                         aTCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATcgcg                                 <  2:852932/94‑1 (MQ=255)
                                                          tCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCgg                                <  2:3771385/94‑1 (MQ=255)
                                                           cGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGt                               <  1:10011155/94‑1 (MQ=255)
                                                            gCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTc                              <  2:2712363/94‑1 (MQ=255)
                                                             cccTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTca                             <  1:7111087/94‑1 (MQ=255)
                                                              ccTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTcac                            >  1:5915446/1‑94 (MQ=255)
                                                              ccTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTcac                            <  1:1993333/94‑1 (MQ=255)
                                                               cTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTcaca                           >  1:3662766/1‑94 (MQ=255)
                                                               cTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTcaca                           >  2:3872300/1‑94 (MQ=255)
                                                               cTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTcaca                           >  1:3526331/1‑94 (MQ=255)
                                                                tGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACAt                          >  1:3179937/1‑94 (MQ=255)
                                                                 gATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATa                         >  2:8887572/1‑94 (MQ=255)
                                                                 gATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATa                         <  2:1078349/94‑1 (MQ=255)
                                                                  aTCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATATCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAg                        <  1:643137/94‑1 (MQ=255)
                                                                   tCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAgg                       >  2:6452262/1‑94 (MQ=255)
                                                                    cGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAggg                      >  1:8108202/1‑94 (MQ=255)
                                                                    cGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAggg                      <  1:3268063/94‑1 (MQ=255)
                                                                     gAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAgggg                     >  2:6599320/1‑94 (MQ=255)
                                                                     gAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAAATGGCTGTTGTACTGAATCAAATTAATCGCGGTCAAATAgggg                     >  2:1732038/1‑94 (MQ=255)
                                                                        aaaaaTCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCg                  <  2:242899/94‑1 (MQ=255)
                                                                        aaaaaTCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCg                  >  2:954574/1‑94 (MQ=255)
                                                                        aaaaaTCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCg                  <  2:4165958/94‑1 (MQ=255)
                                                                          aaaTCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGt                <  1:6274523/94‑1 (MQ=255)
                                                                            aTCATATCGCCAGGCGCCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGttt              <  2:4303967/94‑1 (MQ=255)
                                                                             tCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGtttt             >  2:5800894/1‑94 (MQ=255)
                                                                             tCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGtttt             >  2:5679102/1‑94 (MQ=255)
                                                                              cATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACCGAATCAAATTAATCGCGGTCACATAGGGGCCGGTtctc            >  1:9379952/1‑94 (MQ=255)
                                                                               atatCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTcc           <  1:3825895/94‑1 (MQ=255)
                                                                               atatCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTcc           >  2:3597047/1‑94 (MQ=255)
                                                                                tatCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTccc          <  1:9673555/94‑1 (MQ=255)
                                                                                tatCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTcca          >  1:5472175/1‑93 (MQ=255)
                                                                                  tCGCCAGGCGGCGCGTGGTGTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGt        <  1:1677049/94‑1 (MQ=255)
                                                                                    gCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTc      <  2:6869456/94‑1 (MQ=255)
                                                                                    gCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTc      <  2:524016/94‑1 (MQ=255)
                                                                                     ccAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTcc     >  2:1846476/1‑94 (MQ=255)
                                                                                     ccAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTcc     >  1:6221545/1‑94 (MQ=255)
                                                                                     ccAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTcc     >  2:609778/1‑94 (MQ=255)
                                                                                     ccAGGCGGCGCCTGGTTTATGTCCTAGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTcc     >  1:10151354/1‑94 (MQ=255)
                                                                                      cAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATCGGGGCCGGTTTTCCCCTTccc    >  2:8159107/1‑94 (MQ=255)
                                                                                      cAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTccc    >  1:496734/1‑94 (MQ=255)
                                                                                      cAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTccc    <  1:3574484/94‑1 (MQ=255)
                                                                                      cAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTccc    >  2:5564908/1‑94 (MQ=255)
                                                                                           |                                                                                          
GGCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCAGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCCCT  >  NC_000913/2332488‑2332669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGGCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCAGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCCCTGAT  >  NC_000913/2332486‑2332672
                                                                                             |                                                                                             
GTGGCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCG                                                                                               >  SRR4302545.10288889/1‑94 (MQ=60)
GTGGCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCG                                                                                               <  SRR4302545.3917098/94‑1 (MQ=60)
GTGGCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCG                                                                                               <  SRR4302545.4124697/94‑1 (MQ=60)
 TGGCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGG                                                                                              >  SRR4302545.6347918/1‑94 (MQ=60)
  GGCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGC                                                                                             >  SRR4302545.6225583/1‑94 (MQ=60)
  GGCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGC                                                                                             <  SRR4302545.7610759/94‑1 (MQ=60)
  GGCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGC                                                                                             >  SRR4302545.1946103/1‑94 (MQ=60)
   GCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCG                                                                                            <  SRR4302545.1697313/94‑1 (MQ=60)
      CTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCTCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCT                                                                                         <  SRR4302545.6870281/94‑1 (MQ=60)
      CTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGTAAAAAATCATATCGCCAGGCGGCGCCT                                                                                         >  SRR4302545.5976100/1‑94 (MQ=60)
      CTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCT                                                                                         >  SRR4302545.263639/1‑94 (MQ=60)
      CTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCT                                                                                         >  SRR4302545.1327184/1‑94 (MQ=60)
      CTTCCGGTGTCGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCT                                                                                         >  SRR4302545.7236758/1‑94 (MQ=60)
        TCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGG                                                                                       <  SRR4302545.2187946/94‑1 (MQ=60)
          CGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTT                                                                                     >  SRR4302545.8112854/1‑94 (MQ=60)
           GGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTT                                                                                    >  SRR4302545.4506745/1‑94 (MQ=60)
           GGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTT                                                                                    >  SRR4302545.1624720/1‑94 (MQ=60)
           GGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTT                                                                                    >  SRR4302545.4506747/1‑94 (MQ=60)
              GTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATG                                                                                 <  SRR4302545.3943199/94‑1 (MQ=60)
               TGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGT                                                                                <  SRR4302545.9916584/94‑1 (MQ=60)
                 GTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCC                                                                              <  SRR4302545.4316721/94‑1 (MQ=60)
                  TAGATGCCGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCAGCGCCTGGTTTATGTCCT                                                                             <  SRR4302545.5652176/94‑1 (MQ=60)
                   AGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTG                                                                            >  SRR4302545.10514648/1‑94 (MQ=60)
                   AGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTG                                                                            <  SRR4302545.4803623/94‑1 (MQ=60)
                    aagGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGG                                                                           <  SRR4302545.8032198/91‑1 (MQ=60)
                       TACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATAGAAAAAAATCATACCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCA                                                                        <  SRR4302545.10264086/94‑1 (MQ=60)
                       GACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCA                                                                        >  SRR4302545.137089/1‑94 (MQ=60)
                         CGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAAT                                                                      >  SRR4302545.435338/1‑94 (MQ=60)
                         CGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGACAGGCGGCGCCTGGTTTATGTCCTGGCCAAT                                                                      >  SRR4302545.9333857/1‑94 (MQ=60)
                          GTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATA                                                                     <  SRR4302545.8028549/94‑1 (MQ=60)
                          GTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATA                                                                     <  SRR4302545.522176/94‑1 (MQ=60)
                          GTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATA                                                                     <  SRR4302545.5733902/94‑1 (MQ=60)
                          GTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATA                                                                     <  SRR4302545.4841173/94‑1 (MQ=60)
                           TAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTTCTTGCCAATAA                                                                    >  SRR4302545.1012258/1‑94 (MQ=60)
                            AACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAA                                                                   >  SRR4302545.6630573/1‑94 (MQ=60)
                             ACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAAC                                                                  <  SRR4302545.4079265/94‑1 (MQ=60)
                               GCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTG                                                                <  SRR4302545.10676927/94‑1 (MQ=60)
                               GCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGATTATGTCCTGGCCAATAAACTG                                                                >  SRR4302545.5804326/1‑94 (MQ=60)
                                CCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGG                                                               <  SRR4302545.1150211/94‑1 (MQ=60)
                                  CCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCAGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCT                                                             <  SRR4302545.7132509/94‑1 (MQ=60)
                                   CATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTG                                                            >  SRR4302545.10051816/1‑94 (MQ=60)
                                    ATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGT                                                           <  SRR4302545.10867295/94‑1 (MQ=60)
                                    ATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGT                                                           <  SRR4302545.6714396/94‑1 (MQ=60)
                                    ATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGT                                                           <  SRR4302545.6714395/94‑1 (MQ=60)
                                    ATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGT                                                           <  SRR4302545.6099364/94‑1 (MQ=60)
                                    ATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGT                                                           >  SRR4302545.1952152/1‑94 (MQ=60)
                                      CCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTG                                                         <  SRR4302545.5019243/94‑1 (MQ=60)
                                      CCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTG                                                         <  SRR4302545.3982417/94‑1 (MQ=60)
                                      CCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTG                                                         <  SRR4302545.277797/94‑1 (MQ=60)
                                      CCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTG                                                         >  SRR4302545.9847483/1‑94 (MQ=60)
                                       CAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGT                                                        >  SRR4302545.8514959/1‑94 (MQ=60)
                                        AGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTC                                                       >  SRR4302545.3689152/1‑94 (MQ=60)
                                        AGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTA                                                       <  SRR4302545.1683058/94‑1 (MQ=60)
                                         GACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTAC                                                      <  SRR4302545.3588657/94‑1 (MQ=60)
                                          ACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACT                                                     <  SRR4302545.1077461/94‑1 (MQ=60)
                                           CCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTG                                                    >  SRR4302545.3772403/1‑94 (MQ=60)
                                           CCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTG                                                    <  SRR4302545.8454847/94‑1 (MQ=60)
                                            CATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGTCCAATAAACTGGCTGTTGTTCTGA                                                   >  SRR4302545.1354347/1‑94 (MQ=60)
                                             ATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAA                                                  <  SRR4302545.7690886/94‑1 (MQ=60)
                                             ATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAA                                                  >  SRR4302545.4198630/1‑94 (MQ=60)
                                             ATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAA                                                  <  SRR4302545.3629493/94‑1 (MQ=60)
                                               TAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATC                                                <  SRR4302545.927697/94‑1 (MQ=60)
                                               TAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATC                                                >  SRR4302545.3902799/1‑94 (MQ=60)
                                               TAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATC                                                <  SRR4302545.10172216/94‑1 (MQ=60)
                                               TAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATC                                                <  SRR4302545.7107281/94‑1 (MQ=60)
                                               TAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCGGGATTATGTCCTGGCtaatagaatagctgcggtattgaatc                                                >  SRR4302545.6092547/1‑68 (MQ=60)
                                                AAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCA                                               <  SRR4302545.6903609/94‑1 (MQ=60)
                                                 AGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAA                                              >  SRR4302545.6594165/1‑94 (MQ=60)
                                                 AGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAA                                              >  SRR4302545.6594464/1‑94 (MQ=60)
                                                 AGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAA                                              <  SRR4302545.10723059/94‑1 (MQ=60)
                                                  GTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAA                                             <  SRR4302545.945372/94‑1 (MQ=60)
                                                    GCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCTCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATT                                           <  SRR4302545.3034717/94‑1 (MQ=60)
                                                    GCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATT                                           >  SRR4302545.1604999/1‑94 (MQ=60)
                                                     CTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTA                                          <  SRR4302545.10074040/94‑1 (MQ=60)
                                                      TGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAA                                         >  SRR4302545.9237307/1‑94 (MQ=60)
                                                      TGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAA                                         >  SRR4302545.5202847/1‑94 (MQ=60)
                                                      TGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGAGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAA                                         >  SRR4302545.2828288/1‑94 (MQ=60)
                                                       GGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAAT                                        <  SRR4302545.5439201/94‑1 (MQ=60)
                                                        GGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATC                                       <  SRR4302545.9460062/94‑1 (MQ=60)
                                                        GGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATC                                       >  SRR4302545.4475257/1‑94 (MQ=60)
                                                        GGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATC                                       >  SRR4302545.1588728/1‑94 (MQ=60)
                                                          CATCGCCCTGATCGAAAAAAATCATATCGCCCAGCGGTGCCTGGTTTATGTCCTAGCCAATAAACTGGCTGTTGTTCTGAATCAAAataatacc                                     >  SRR4302545.4245805/1‑86 (MQ=60)
                                                            TCGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGG                                   <  SRR4302545.5423445/94‑1 (MQ=60)
                                                             CGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGT                                  <  SRR4302545.10307917/94‑1 (MQ=60)
                                                             CGCCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGT                                  <  SRR4302545.926974/94‑1 (MQ=60)
                                                               CCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCA                                <  SRR4302545.7308104/94‑1 (MQ=60)
                                                               CCCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCA                                >  SRR4302545.3239441/1‑94 (MQ=60)
                                                                CCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCAC                               <  SRR4302545.2042639/94‑1 (MQ=60)
                                                                CCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCAC                               >  SRR4302545.3178927/1‑94 (MQ=60)
                                                                CCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCAC                               <  SRR4302545.7000420/94‑1 (MQ=60)
                                                                CCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCAC                               >  SRR4302545.6075001/1‑94 (MQ=60)
                                                                CCTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTAGCCAATAAACTGGCTGTTGTACTGGATCAAATTAATCagcgtcac                               >  SRR4302545.2353849/1‑86 (MQ=60)
                                                                 CTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACA                              >  SRR4302545.3752274/1‑94 (MQ=60)
                                                                 CTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACA                              >  SRR4302545.2483912/1‑94 (MQ=60)
                                                                 CTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACA                              >  SRR4302545.4520983/1‑94 (MQ=60)
                                                                 CTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACA                              <  SRR4302545.6420212/94‑1 (MQ=60)
                                                                 CTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACA                              <  SRR4302545.6420213/94‑1 (MQ=60)
                                                                 CTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACA                              >  SRR4302545.2991201/1‑94 (MQ=60)
                                                                 CTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACA                              <  SRR4302545.1043705/94‑1 (MQ=60)
                                                                 CTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACA                              >  SRR4302545.3612781/1‑94 (MQ=60)
                                                                 CTGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGGTGTACTGAATCAAATTAATCGCGGTCACA                              >  SRR4302545.8336975/1‑94 (MQ=60)
                                                                  TGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACAT                             <  SRR4302545.1480078/94‑1 (MQ=60)
                                                                  TGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACAT                             <  SRR4302545.4287322/94‑1 (MQ=60)
                                                                  TGATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACAT                             >  SRR4302545.3257962/1‑94 (MQ=60)
                                                                    ATCGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATATCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAG                           <  SRR4302545.657499/94‑1 (MQ=60)
                                                                      CGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGG                         <  SRR4302545.3348178/94‑1 (MQ=60)
                                                                      CGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGG                         <  SRR4302545.3393864/94‑1 (MQ=60)
                                                                      CGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGG                         <  SRR4302545.6485866/94‑1 (MQ=60)
                                                                      CGAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGG                         >  SRR4302545.8342075/1‑94 (MQ=60)
                                                                       GAAAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGG                        >  SRR4302545.325206/1‑94 (MQ=60)
                                                                         AAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCC                      <  SRR4302545.1981442/94‑1 (MQ=60)
                                                                         AAAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCC                      <  SRR4302545.10140589/94‑1 (MQ=60)
                                                                          AAAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCG                     >  SRR4302545.6526525/1‑94 (MQ=60)
                                                                           AAAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGG                    >  SRR4302545.2868726/1‑94 (MQ=60)
                                                                            AAATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGT                   <  SRR4302545.6445595/94‑1 (MQ=60)
                                                                             AATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTT                  >  SRR4302545.6131923/1‑94 (MQ=60)
                                                                              ATCATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTT                 >  SRR4302545.6872269/1‑94 (MQ=60)
                                                                                CATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACCGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTCTC               >  SRR4302545.9655210/1‑94 (MQ=60)
                                                                                 ATATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCC              <  SRR4302545.3921169/94‑1 (MQ=60)
                                                                                  TATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCC             <  SRR4302545.9960524/94‑1 (MQ=60)
                                                                                  TATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCC             >  SRR4302545.3655597/1‑94 (MQ=60)
                                                                                  TATCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCA             >  SRR4302545.5615822/1‑94 (MQ=60)
                                                                                    TCGCCAGGCGGCGCGTGGTGTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGT           <  SRR4302545.1716766/94‑1 (MQ=60)
                                                                                    TCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGT           <  SRR4302545.1716767/94‑1 (MQ=60)
                                                                                    TCGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGT           <  SRR4302545.3991831/94‑1 (MQ=60)
                                                                                     CGCCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTT          >  SRR4302545.6288263/1‑94 (MQ=60)
                                                                                       CCAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCC        >  SRR4302545.6391003/1‑94 (MQ=60)
                                                                                       CCAGGCGGCGCCTGGTTTATGTCCTAGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCC        >  SRR4302545.10451512/1‑94 (MQ=60)
                                                                                        CAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCC       >  SRR4302545.507851/1‑94 (MQ=60)
                                                                                        CAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCC       <  SRR4302545.10320238/94‑1 (MQ=60)
                                                                                        CAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCC       <  SRR4302545.3661760/94‑1 (MQ=60)
                                                                                        CAGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCC       >  SRR4302545.7474867/1‑94 (MQ=60)
                                                                                         AGGCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCCC      >  SRR4302545.9406755/1‑94 (MQ=60)
                                                                                           GCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCCCTG    <  SRR4302545.8886374/94‑1 (MQ=60)
                                                                                           GCGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCCCTG    <  SRR4302545.2740604/94‑1 (MQ=60)
                                                                                            CGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCCCTGA   >  SRR4302545.3957281/1‑94 (MQ=60)
                                                                                            CGGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCCCTGA   <  SRR4302545.5471700/94‑1 (MQ=60)
                                                                                             GGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCCCTGAT  >  SRR4302545.897940/1‑94 (MQ=60)
                                                                                             GGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCCCTGAT  <  SRR4302545.6027744/94‑1 (MQ=60)
                                                                                             GGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCCCTGAT  <  SRR4302545.8590895/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTGGCGCTTCCGGTGTGGTAGATGACGTAACGCCCCATCCAGACCATTAAGTGCTGGGCATCGCCCTGATCGAAAAAAATCATATCGCCAGGCAGCGCCTGGTTTATGTCCTGGCCAATAAACTGGCTGTTGTACTGAATCAAATTAATCGCGGTCACATAGGGGCCGGTTTTCCCGTTCCCCTGAT  >  NC_000913/2332486‑2332672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑