Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,352,497	A→G	A71A (GCA→GCG)	glpB →	sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), membrane anchor subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,352,497	0	A	G	96.2%	581.1 / 6.5	188	A71A (GCA→GCG)	glpB	sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), membrane anchor subunit
Reads supporting (aligned to +/- strand): ref base A (4/3); new base G (112/67); total (116/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 219 total aligned reads displayed.

ATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCA  >  NC_000913/2352407‑2352586

aTCCGGAGCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGcc                                                                                         <  2:4981144/93‑1 (MQ=255)

 tCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCa                                                                                        <  2:1264284/93‑1 (MQ=255)

  ccGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAg                                                                                       >  1:2583919/1‑93 (MQ=255)

  ccGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAg                                                                                       >  1:141228/1‑93 (MQ=255)

   cGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGc                                                                                      >  1:1031821/1‑93 (MQ=255)

   cGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGc                                                                                      <  1:4715950/93‑1 (MQ=255)

   cGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGc                                                                                      >  1:4466655/1‑93 (MQ=255)

    ggATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGcc                                                                                     >  1:1574730/1‑93 (MQ=255)

    ggATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGcc                                                                                     >  2:3976556/1‑93 (MQ=255)

     gATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGccc                                                                                    <  2:580171/93‑1 (MQ=255)

      aTCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCa                                                                                   <  2:3540360/93‑1 (MQ=255)

       tCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCAt                                                                                  >  2:5775072/1‑93 (MQ=255)

       tCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCAt                                                                                  >  2:5881394/1‑93 (MQ=255)

       tCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCAt                                                                                  >  2:2041982/1‑93 (MQ=255)

       tCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCAt                                                                                  >  1:3230016/1‑93 (MQ=255)

        cGCTCGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATc                                                                                 <  2:3168594/93‑1 (MQ=255)

         gCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATcc                                                                                <  1:6473834/93‑1 (MQ=255)

           tGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCtt                                                                              >  2:3000210/1‑93 (MQ=255)

             gATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTAc                                                                            >  2:5674056/1‑93 (MQ=255)

             gATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTAc                                                                            >  1:5459858/1‑93 (MQ=255)

             gATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTAc                                                                            <  1:3519852/93‑1 (MQ=255)

                  gCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCt                                                                       <  1:4866165/93‑1 (MQ=255)

                   cTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCtt                                                                      <  1:3850098/93‑1 (MQ=255)

                   cTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCtt                                                                      <  2:237245/93‑1 (MQ=255)

                   cTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTAATCCCtt                                                                      >  1:1957842/1‑93 (MQ=255)

                    tGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTtc                                                                     <  2:4892607/93‑1 (MQ=255)

                    tGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTtc                                                                     <  1:4263663/93‑1 (MQ=255)

                     gAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTtct                                                                    >  2:4110216/1‑93 (MQ=255)

                       gCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCg                                                                  >  2:1708951/1‑93 (MQ=255)

                       gCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCg                                                                  >  2:3606091/1‑93 (MQ=255)

                        ccATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGTGTCAGCAGGCGCCAGCCCAGCCTTACTCCCTTCTCGa                                                                 <  1:6403603/93‑1 (MQ=255)

                        ccATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGa                                                                 <  2:4199153/93‑1 (MQ=255)

                        ccATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGa                                                                 >  1:4420261/1‑93 (MQ=255)

                          aTCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGc                                                               >  1:819789/1‑93 (MQ=255)

                          aTCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGc                                                               >  1:4040507/1‑93 (MQ=255)

                           tCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGcc                                                              >  2:2576974/1‑93 (MQ=255)

                           tCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGcc                                                              >  1:2336383/1‑93 (MQ=255)

                           tCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGcc                                                              >  2:4764692/1‑93 (MQ=255)

                             tGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCcac                                                            >  1:122681/1‑93 (MQ=255)

                              gctaGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATTTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCcaca                                                           <  2:3920158/90‑1 (MQ=255)

                              gCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCcaca                                                           >  2:1181803/1‑93 (MQ=255)

                               ccAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCCCCAGCCCATCCTTCCTCCCTTCTCGAGCCACaa                                                          <  2:4302844/93‑1 (MQ=255)

                                 aGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAAcg                                                        >  2:1438826/1‑93 (MQ=255)

                                  gATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAAcgc                                                       >  1:2826075/1‑93 (MQ=255)

                                    tGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGt                                                     >  1:1163052/1‑93 (MQ=255)

                                      gTCAACCGGTGACAGACATTCACAGCGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTAGTCCCTTCTCGAGCCACAACGCGTGc                                                   >  2:5251073/1‑93 (MQ=255)

                                       tCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCt                                                  <  1:202745/93‑1 (MQ=255)

                                         aaCCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCg                                                <  1:4230814/93‑1 (MQ=255)

                                          aCCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGa                                               <  2:1615796/93‑1 (MQ=255)

                                           ccGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGAt                                              >  1:5310976/1‑93 (MQ=255)

                                           ccGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGAt                                              >  2:5326923/1‑93 (MQ=255)

                                           ccGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGAt                                              <  2:1047857/93‑1 (MQ=255)

                                           ccGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGAt                                              <  1:2379776/93‑1 (MQ=255)

                                              gTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGAtctc                                           <  2:39460/93‑1 (MQ=255)

                                               tGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCg                                          <  1:11757/93‑1 (MQ=255)

                                               tGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCg                                          >  1:2083355/1‑93 (MQ=255)

                                                  cagacaTTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTCCTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCtt                                       <  2:593400/93‑1 (MQ=255)

                                                  cagacaTTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCtt                                       >  2:658690/1‑93 (MQ=255)

                                                  cagacaTTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCtt                                       >  2:5646165/1‑93 (MQ=255)

                                                  cagacaTTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCtt                                       >  1:1693353/1‑93 (MQ=255)

                                                   agacaTTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATATCGCTTg                                      <  2:6408883/93‑1 (MQ=255)

                                                    gacaTTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGc                                     >  1:4855503/1‑93 (MQ=255)

                                                    gacaTTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGc                                     >  1:3657598/1‑93 (MQ=255)

                                                      caTTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCa                                   >  1:2772858/1‑93 (MQ=255)

                                                       aTTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAg                                  <  1:6135602/93‑1 (MQ=255)

                                                        ttCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAgg                                 >  2:6336891/1‑93 (MQ=255)

                                                        ttCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGATCGATCTCGCTTGCCAgg                                 >  1:2321292/1‑93 (MQ=255)

                                                           acaGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGgcgc                              >  2:4640408/1‑93 (MQ=255)

                                                            caGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCa                             <  1:1071620/93‑1 (MQ=255)

                                                            caGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCa                             <  1:6168520/93‑1 (MQ=255)

                                                             aGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTCCCCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAg                            <  2:552583/93‑1 (MQ=255)

                                                             aGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAg                            >  2:2799514/1‑93 (MQ=255)

                                                              gTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAgg                           >  2:5389519/1‑93 (MQ=255)

                                                              gTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAgg                           <  2:4674472/93‑1 (MQ=255)

                                                                ggACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCTAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCa                         <  1:4909413/93‑1 (MQ=255)

                                                                ggACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCa                         <  2:6253626/93‑1 (MQ=255)

                                                                   cTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTa                      <  1:5802091/93‑1 (MQ=255)

                                                                    tGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTaa                     >  2:1369111/1‑93 (MQ=255)

                                                                        aTCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGc                 >  1:2869708/1‑93 (MQ=255)

                                                                        aTCTTTGCGTCAGCAGGCCCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGc                 <  2:715536/93‑1 (MQ=255)

                                                                        aTCTTTGCGTAAGCAGGCTCCAGCCCATCCTTACTCCCTCCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGc                 <  2:920571/93‑1 (MQ=255)

                                                                          cTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTg               >  2:879362/1‑93 (MQ=255)

                                                                           tttGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGa              <  2:5098219/93‑1 (MQ=255)

                                                                             tGCGTCAGCAGGCCCCAGCCCACCCTCACTCCCTTCCCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGaaa            <  2:2487466/93‑1 (MQ=255)

                                                                             tGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGaaa            <  1:480677/93‑1 (MQ=255)

                                                                              gCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAg           <  1:851828/93‑1 (MQ=255)

                                                                               cGTCAGCAGGCGCCAGCCCATCCTTCCTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGc          <  2:1396041/93‑1 (MQ=255)

                                                                                gTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCg         >  1:5923794/1‑93 (MQ=255)

                                                                                gTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCg         <  1:327997/93‑1 (MQ=255)

                                                                                ctCAGCAGCCGCCAGCCCATCCTTCCTCCCTCCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCg         <  1:1373292/92‑1 (MQ=255)

                                                                                 tCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCgg        <  2:3749276/93‑1 (MQ=255)

                                                                                 tCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCgg        >  1:2494088/1‑93 (MQ=255)

                                                                                 gcaccagGCCCCAGCGCATCCTTACGCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCgg        <  1:938616/89‑1 (MQ=255)

                                                                                  cagcagGCGCCAGCCCACCCTTCCTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGt       <  2:3802261/93‑1 (MQ=255)

                                                                                   agcagGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTg      >  1:773300/1‑93 (MQ=255)

                                                                                   agcagGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTg      >  1:1334883/1‑93 (MQ=255)

                                                                                   agcagGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTg      >  1:1001795/1‑93 (MQ=255)

                                                                                    gcagGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgc     >  2:507243/1‑93 (MQ=255)

                                                                                      agGCGCCAGCGCATCCTTACTCCCTTCCCGAACCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc   <  1:5635801/93‑1 (MQ=255)

                                                                                       ggCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCa  >  1:3947134/1‑93 (MQ=255)

ATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCA  >  NC_000913/2352407‑2352586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATT  >  NC_000913/2352404‑2352589

CTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCG                                                                                              >  SRR4302537.6436165/1‑94 (MQ=60)

    TCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAG                                                                                          >  SRR4302537.142232/1‑94 (MQ=60)

    TCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAG                                                                                          >  SRR4302537.2602004/1‑94 (MQ=60)

     CCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGC                                                                                         >  SRR4302537.1038930/1‑94 (MQ=60)

     CCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGC                                                                                         >  SRR4302537.4497546/1‑94 (MQ=60)

     CCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGC                                                                                         >  SRR4302537.1191533/1‑94 (MQ=60)

      CGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCC                                                                                        <  SRR4302537.4748564/94‑1 (MQ=60)

      CGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCC                                                                                        >  SRR4302537.1585714/1‑94 (MQ=60)

       GGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCC                                                                                       >  SRR4302537.137069/1‑94 (MQ=60)

         ATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCAT                                                                                     >  SRR4302537.593199/1‑94 (MQ=60)

         ATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCAT                                                                                     >  SRR4302537.2815989/1‑94 (MQ=60)

         ATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCAT                                                                                     >  SRR4302537.3252781/1‑94 (MQ=60)

         ATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCAT                                                                                     >  SRR4302537.3896782/1‑94 (MQ=60)

           CGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCC                                                                                   >  SRR4302537.1828652/1‑94 (MQ=60)

            GCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATGCT                                                                                  >  SRR4302537.4855901/1‑94 (MQ=60)

            GCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCT                                                                                  <  SRR4302537.6517765/94‑1 (MQ=60)

            GCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATACT                                                                                  >  SRR4302537.1418223/1‑94 (MQ=60)

             CTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTT                                                                                 >  SRR4302537.1042349/1‑94 (MQ=60)

              TGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTA                                                                                <  SRR4302537.467413/94‑1 (MQ=60)

               GGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTAC                                                                               >  SRR4302537.1311766/1‑94 (MQ=60)

               GGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTAC                                                                               >  SRR4302537.5497103/1‑94 (MQ=60)

                GATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACT                                                                              <  SRR4302537.3544758/94‑1 (MQ=60)

                     GCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTT                                                                         <  SRR4302537.4899744/94‑1 (MQ=60)

                     GCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTAATCCCTT                                                                         >  SRR4302537.1971465/1‑94 (MQ=60)

                      CTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTC                                                                        <  SRR4302537.2946232/94‑1 (MQ=60)

                      CTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTC                                                                        <  SRR4302537.3877059/94‑1 (MQ=60)

                       TGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCT                                                                       <  SRR4302537.4293288/94‑1 (MQ=60)

                       TGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCT                                                                       <  SRR4302537.6125119/94‑1 (MQ=60)

                          GCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGA                                                                    >  SRR4302537.4450779/1‑94 (MQ=60)

                           CCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGTGTCAGCAGGCGCCAGCCCAGCCTTACTCCCTTCTCGAG                                                                   <  SRR4302537.6447189/94‑1 (MQ=60)

                            CATCTTCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGC                                                                  >  SRR4302537.490186/1‑94 (MQ=60)

                            CATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGC                                                                  >  SRR4302537.4068772/1‑94 (MQ=60)

                            CATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGC                                                                  >  SRR4302537.825419/1‑94 (MQ=60)

                             ATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCC                                                                 >  SRR4302537.2352856/1‑94 (MQ=60)

                              TCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCA                                                                <  SRR4302537.620056/94‑1 (MQ=60)

                               CTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCAC                                                               >  SRR4302537.123566/1‑94 (MQ=60)

                                TGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACA                                                              >  SRR4302537.5750964/1‑94 (MQ=60)

                                 GCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAA                                                             <  SRR4302537.1342899/94‑1 (MQ=60)

                                  CCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAAC                                                            >  SRR4302537.6462982/1‑94 (MQ=60)

                                    AGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGC                                                          >  SRR4302537.2845830/1‑94 (MQ=60)

                                    AGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGC                                                          <  SRR4302537.1120722/94‑1 (MQ=60)

                                     GATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCG                                                         <  SRR4302537.6409771/94‑1 (MQ=60)

                                     GATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCG                                                         <  SRR4302537.2008873/94‑1 (MQ=60)

                                      ATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGT                                                        >  SRR4302537.1171139/1‑94 (MQ=60)

                                         GTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCT                                                     >  SRR4302537.5056207/1‑94 (MQ=60)

                                          TCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTC                                                    <  SRR4302537.204197/94‑1 (MQ=60)

                                           CAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCG                                                   <  SRR4302537.5408769/94‑1 (MQ=60)

                                           CAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCG                                                   >  SRR4302537.2068665/1‑94 (MQ=60)

                                            AACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGA                                                  <  SRR4302537.4260267/94‑1 (MQ=60)

                                             ACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGAT                                                 >  SRR4302537.5347459/1‑94 (MQ=60)

                                              cgggtgacaaaatTTCACAGTGGACTGAATTCTTTGCGTCAGCAGGCGCCAGCCCATCCTTCCCCCCTTCTCGAGCCACAACGCGTGCTCGATC                                                <  SRR4302537.4463768/81‑1 (MQ=60)

                                              cgggggcCAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATC                                                <  SRR4302537.5464155/87‑1 (MQ=60)

                                              ccggtgccgaACATTCACAGT‑GCCTGGACTCTTGGCGTCAGCAGGCCCGAGCCCATCCTTCCCCCCTTCCCGAGCCACAACGCGTGCTCGATCT                                               <  SRR4302537.50536/84‑1 (MQ=31)

                                              CCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATC                                                <  SRR4302537.2396543/94‑1 (MQ=60)

                                               CGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCT                                               >  SRR4302537.5622208/1‑94 (MQ=60)

                                               CGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCCTGCTCGATCT                                               >  SRR4302537.1851363/1‑94 (MQ=60)

                                                GGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTC                                              <  SRR4302537.3720075/94‑1 (MQ=60)

                                                 GTGACAGACATTCACATTGGACGGAATTCTTTGCGTCAGCAGGCCCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCG                                             <  SRR4302537.4532171/94‑1 (MQ=60)

                                                 GTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCG                                             >  SRR4302537.2098032/1‑94 (MQ=60)

                                                  TGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGC                                            >  SRR4302537.3948212/1‑94 (MQ=60)

                                                  TGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGC                                            <  SRR4302537.11842/94‑1 (MQ=60)

                                                   GACAGACATTCTCAGTGGACTGGAATCTTGGCGTCAGCAGGCGCCAGCCCATCCTTACCCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCT                                           <  SRR4302537.4834833/94‑1 (MQ=60)

                                                   GACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTCCTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCT                                           <  SRR4302537.4146919/94‑1 (MQ=60)

                                                   GACAGACATTCACAGTGGACTGGAATCTTGGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCT                                           <  SRR4302537.1554784/94‑1 (MQ=60)

                                                   GACAGACATTCACAGTGGACGGGAATCTTGGCGGCAGCAGGCCCCAGCCAATCCTTTCTCCCTTCTGGAGCCACAACGCGTGCTCGATCTCGCT                                           <  SRR4302537.1341289/94‑1 (MQ=60)

                                                    ACAGAGATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTT                                          <  SRR4302537.2380033/94‑1 (MQ=60)

                                                    ACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTCCTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTT                                          <  SRR4302537.692113/94‑1 (MQ=60)

                                                    ACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTT                                          >  SRR4302537.1705204/1‑94 (MQ=60)

                                                      AGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGC                                        >  SRR4302537.4889006/1‑94 (MQ=60)

                                                      AGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGC                                        >  SRR4302537.2372536/1‑94 (MQ=60)

                                                      AGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGC                                        >  SRR4302537.3683340/1‑94 (MQ=60)

                                                       GACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCC                                       >  SRR4302537.4320741/1‑94 (MQ=60)

                                                        ACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCA                                      >  SRR4302537.2792236/1‑94 (MQ=60)

                                                          ATTCACAGTGGCCTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTCCTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGG                                    <  SRR4302537.2537110/94‑1 (MQ=60)

                                                          ATTCACAGTGGACTGGAATCTTTGCGTCGGCAGCCCCCACCCCATCCTGACTCCCTTCCGGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGG                                    <  SRR4302537.2873743/94‑1 (MQ=60)

                                                          ATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGG                                    <  SRR4302537.6177782/94‑1 (MQ=60)

                                                          ATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGATCGATCTCGCTTGCCAGG                                    >  SRR4302537.2337682/1‑94 (MQ=60)

                                                            TCACAGTGGACGGGAATCTTGGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCG                                  <  SRR4302537.4895690/94‑1 (MQ=60)

                                                             CACAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGC                                 >  SRR4302537.3616117/1‑94 (MQ=60)

                                                               CAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTCCTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAG                               <  SRR4302537.2352756/94‑1 (MQ=60)

                                                               CAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAG                               <  SRR4302537.6210996/94‑1 (MQ=60)

                                                               CAGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAG                               <  SRR4302537.1079007/94‑1 (MQ=60)

                                                                AGTGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGG                              >  SRR4302537.4292745/1‑94 (MQ=60)

                                                                  TGGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCA                            >  SRR4302537.6128956/1‑94 (MQ=60)

                                                                   GGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCTAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCAT                           <  SRR4302537.4943302/94‑1 (MQ=60)

                                                                   GGACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCAT                           <  SRR4302537.4067840/94‑1 (MQ=60)

                                                                     ACTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTA                         >  SRR4302537.925648/1‑94 (MQ=60)

                                                                      CTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAA                        >  SRR4302537.4465740/1‑94 (MQ=60)

                                                                      CTGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAA                        <  SRR4302537.5841871/94‑1 (MQ=60)

                                                                       TGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAAT                       >  SRR4302537.4913199/1‑94 (MQ=60)

                                                                       TGGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAAT                       >  SRR4302537.851183/1‑94 (MQ=60)

                                                                        GGAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATC                      >  SRR4302537.3291160/1‑94 (MQ=60)

                                                                         GAATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCACTAATCG                     >  SRR4302537.4468922/1‑94 (MQ=60)

                                                                         GAATCTTTGCGTCAGCAGGCGCCACCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCG                     <  SRR4302537.822904/94‑1 (MQ=60)

                                                                          AATCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGC                    >  SRR4302537.2889850/1‑94 (MQ=60)

                                                                            TCTTTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTG                  >  SRR4302537.2279867/1‑94 (MQ=60)

                                                                               TTGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAA               <  SRR4302537.803095/94‑1 (MQ=60)

                                                                                TGCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAG              >  SRR4302537.1683472/1‑94 (MQ=60)

                                                                                TGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAG              <  SRR4302537.483970/94‑1 (MQ=60)

                                                                                 GCGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGC             <  SRR4302537.857665/94‑1 (MQ=60)

                                                                                  CGTCAGCAGGCGCCAGCCCATCCTTCCTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCG            <  SRR4302537.2687137/94‑1 (MQ=60)

                                                                                  CGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCG            >  SRR4302537.996699/1‑94 (MQ=60)

                                                                                  CGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCG            <  SRR4302537.3310191/94‑1 (MQ=60)

                                                                                  CGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCG            >  SRR4302537.5964441/1‑94 (MQ=60)

                                                                                  CGTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCG            >  SRR4302537.2401493/1‑94 (MQ=60)

                                                                                   GTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGG           <  SRR4302537.330273/94‑1 (MQ=60)

                                                                                   GTCAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGG           >  SRR4302537.2511648/1‑94 (MQ=60)

                                                                                   CTCAGCAGCCGCCAGCCCATCCTTCCTCCCTCCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGG           <  SRR4302537.1382881/94‑1 (MQ=60)

                                                                                    gcacCAGGCCCCAGCGCATCCTTACGCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGT          <  SRR4302537.945089/90‑1 (MQ=60)

                                                                                    ACAGCAGGCCCCAGCCCATCCTTGCCCCCTTCCCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGT          <  SRR4302537.1010622/94‑1 (MQ=60)

                                                                                     CAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTG         >  SRR4302537.778598/1‑94 (MQ=60)

                                                                                     CAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTG         >  SRR4302537.1008707/1‑94 (MQ=60)

                                                                                     CAGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTG         >  SRR4302537.1344183/1‑94 (MQ=60)

                                                                                      AGCAGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGC        >  SRR4302537.1179125/1‑94 (MQ=60)

                                                                                      AGCAGGCGCCAGCCCACCCTTACTCCCTCCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGC        <  SRR4302537.2933426/94‑1 (MQ=60)

                                                                                         AGGCGCCAGCGCATCCTTACTCCCTTCCCGAACCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCA     <  SRR4302537.5674369/94‑1 (MQ=60)

                                                                                         AGGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCA     >  SRR4302537.3974740/1‑94 (MQ=60)

                                                                                          GGCGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAA    >  SRR4302537.2589253/1‑94 (MQ=60)

                                                                                            CGCCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATT  >  SRR4302537.6481974/1‑94 (MQ=60)

CTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATT  >  NC_000913/2352404‑2352589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`