Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,416,460	A→G	S461G (AGT→GGT)	yfcC →	putative S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,416,460	0	A	G	96.1%	571.0 / 9.5	180	S461G (AGT→GGT)	yfcC	putative S‑transferase
Reads supporting (aligned to +/- strand): ref base A (3/4); new base G (97/76); total (100/80)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

Only 100 of 184 total aligned reads displayed.

GTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGC  >  NC_000913/2416369‑2416548

gTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAg                                                                                         >  2:2893423/1‑93 (MQ=255)

 ttAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGt                                                                                        >  1:3226930/1‑93 (MQ=255)

    aCCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTcac                                                                                     >  2:2647675/1‑93 (MQ=255)

    aCCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTcac                                                                                     >  2:4087343/1‑93 (MQ=255)

     ccATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTcaca                                                                                    >  1:6359565/1‑93 (MQ=255)

       aTGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCAcatc                                                                                  <  2:3970535/93‑1 (MQ=255)

       aTGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCAcatc                                                                                  <  2:5131657/93‑1 (MQ=255)

        tGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCAcatca                                                                                 >  1:4321179/1‑93 (MQ=255)

        tGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCAcatca                                                                                 <  2:4841809/93‑1 (MQ=255)

          ccGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCAtt                                                                               <  1:4985062/93‑1 (MQ=255)

          ccGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCAtt                                                                               >  2:5336723/1‑93 (MQ=255)

           cGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCAttt                                                                              <  1:160471/93‑1 (MQ=255)

            gTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTa                                                                             >  1:202511/1‑93 (MQ=255)

            gTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTa                                                                             >  2:4594854/1‑93 (MQ=255)

               aCTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTAccc                                                                          >  1:4648701/1‑93 (MQ=255)

                   gCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAAcc                                                                      <  2:6011856/93‑1 (MQ=255)

                     aCCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTc                                                                    >  2:2756615/1‑93 (MQ=255)

                      ccGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCa                                                                   <  1:2459028/93‑1 (MQ=255)

                      ccGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCa                                                                   >  1:6430883/1‑93 (MQ=255)

                      ccGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCa                                                                   >  2:3513163/1‑93 (MQ=255)

                      ccGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCa                                                                   >  2:5569/1‑93 (MQ=255)

                      ccGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCa                                                                   <  1:2008874/93‑1 (MQ=255)

                       cGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAg                                                                  <  2:219597/93‑1 (MQ=255)

                       cGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAg                                                                  <  1:4433496/93‑1 (MQ=255)

                        gCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGc                                                                 >  1:1754235/1‑93 (MQ=255)

                           tGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGcgtc                                                              >  2:4892479/1‑93 (MQ=255)

                            ggCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGcgtcg                                                             >  1:308715/1‑93 (MQ=255)

                              cGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGtt                                                           <  1:142704/93‑1 (MQ=255)

                               gATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTa                                                          >  1:3868717/1‑93 (MQ=255)

                               gATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACACAACCTCAGCGTCGTTa                                                          >  2:2068301/1‑93 (MQ=255)

                                aTCTGTTCCGTGTTAACCGTCAGGTTACCGTGCTGGCCTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTaa                                                         <  2:1379686/93‑1 (MQ=255)

                                aTCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTaa                                                         >  1:3258799/1‑93 (MQ=255)

                                aTCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTaa                                                         >  2:932196/1‑93 (MQ=255)

                                aTCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTaa                                                         <  1:4795401/93‑1 (MQ=255)

                                 tCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAAt                                                        >  2:3184370/1‑93 (MQ=255)

                                    ggTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGc                                                     <  1:1014166/93‑1 (MQ=255)

                                    ggTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGc                                                     <  1:2803089/93‑1 (MQ=255)

                                     gTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCa                                                    <  2:432123/93‑1 (MQ=255)

                                      tCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCaa                                                   <  2:5287080/93‑1 (MQ=255)

                                      tCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCaa                                                   <  2:2851603/93‑1 (MQ=255)

                                        ggTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTTATGGCAACg                                                 >  2:5899504/1‑93 (MQ=255)

                                        ggTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACg                                                 >  2:1929959/1‑93 (MQ=255)

                                         gtgtTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGc                                                >  1:3642752/1‑93 (MQ=255)

                                         gtgtTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGc                                                >  2:4346782/1‑93 (MQ=255)

                                          tgtTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCt                                               >  2:2723233/1‑93 (MQ=255)

                                           gtTAACCGTCAGGTTACGGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTc                                              <  2:1542439/93‑1 (MQ=255)

                                            ttAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCg                                             <  1:4252482/93‑1 (MQ=255)

                                             tAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCgg                                            <  1:6244423/93‑1 (MQ=255)

                                               aCCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGgtg                                          <  2:6205778/93‑1 (MQ=255)

                                                ccGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGgtgt                                         >  1:4448328/1‑93 (MQ=255)

                                                ccGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGgtgt                                         <  1:66782/93‑1 (MQ=255)

                                                ccGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGgtgt                                         >  1:5329016/1‑93 (MQ=255)

                                                 cGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGgtgtg                                        <  2:1802481/93‑1 (MQ=255)

                                                  gTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGgtgtgt                                       <  2:3308417/93‑1 (MQ=255)

                                                  gTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGgtgtgt                                       >  1:2607116/1‑93 (MQ=255)

                                                  gTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGgtgtgt                                       <  2:624387/93‑1 (MQ=255)

                                                    cAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGc                                     <  1:3551456/93‑1 (MQ=255)

                                                     aGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGcg                                    <  1:3916587/93‑2 (MQ=255)

                                                     aGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGcg                                    >  1:4941709/1‑92 (MQ=255)

                                                      ggTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGcgg                                   <  1:1545497/93‑3 (MQ=255)

                                                      ggTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGcgg                                   >  2:866739/1‑91 (MQ=255)

                                                       gTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCggg                                  >  1:2499567/1‑93 (MQ=255)

                                                       gTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCggg                                  <  2:2384874/93‑1 (MQ=255)

                                                         tACCGTGCTGGCGTTCTAGTTTGGTGATGGCTTCGGTCACATCATTTACCCACCCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGt                                <  2:6345516/93‑1 (MQ=255)

                                                         tACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGt                                <  2:1099164/93‑1 (MQ=255)

                                                          aCCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTg                               >  2:2571401/1‑93 (MQ=255)

                                                          aCCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTg                               >  1:1890952/1‑93 (MQ=255)

                                                           ccGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTgg                              >  2:1257307/1‑93 (MQ=255)

                                                           ccGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTgg                              <  2:6472026/93‑1 (MQ=255)

                                                           ccGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTgg                              >  2:763015/1‑93 (MQ=255)

                                                            cGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGa                             >  1:2994185/1‑93 (MQ=255)

                                                              tGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCTACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACt                           >  1:6242432/1‑93 (MQ=255)

                                                              tGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACt                           >  2:3631463/1‑93 (MQ=255)

                                                              tGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACt                           >  1:5998877/1‑93 (MQ=255)

                                                                cTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTaa                                                         <  1:5255296/61‑1 (MQ=255)

                                                                cTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTc                         >  2:4285873/1‑93 (MQ=255)

                                                                cTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTc                         <  2:2342647/93‑1 (MQ=255)

                                                                cTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTc                         <  2:2855720/93‑1 (MQ=255)

                                                                 tGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTcc                        <  1:5179546/93‑1 (MQ=255)

                                                                 tGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTcc                        >  1:2223532/1‑93 (MQ=255)

                                                                     gTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTaa                    >  1:2502323/1‑93 (MQ=255)

                                                                     gTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTaa                    <  2:2227677/93‑1 (MQ=255)

                                                                        ccAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTg                 >  1:3753354/1‑93 (MQ=255)

                                                                        ccAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTg                 <  1:2185470/93‑1 (MQ=255)

                                                                         cAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTgg                >  1:100877/1‑93 (MQ=255)

                                                                         cAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTgg                >  1:902963/1‑93 (MQ=255)

                                                                          aGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGc               >  2:1971936/1‑93 (MQ=255)

                                                                            tttGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTg             >  1:3480498/1‑93 (MQ=255)

                                                                            tttGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTg             <  1:3949585/93‑1 (MQ=255)

                                                                              tGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGaa           <  1:4239373/93‑1 (MQ=255)

                                                                               ggTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAg          >  1:757910/1‑93 (MQ=255)

                                                                                 tGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGt        <  1:5822434/93‑1 (MQ=255)

                                                                                  gATGGCTTCGGTCACATCATTTACCCAACCTGAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTg       <  1:790643/93‑1 (MQ=255)

                                                                                  gATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTg       >  2:5224569/1‑93 (MQ=255)

                                                                                  gATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTg       <  2:1036060/93‑1 (MQ=255)

                                                                                   aTGTCTTCGCTCACATCATTTGCCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTgg      <  2:3584623/93‑1 (MQ=255)

                                                                                   aTGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTgg      <  2:5619086/93‑1 (MQ=255)

                                                                                   aTGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTgg      <  2:2976896/93‑1 (MQ=255)

                                                                                   aTGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTgg      >  1:5667385/1‑93 (MQ=255)

                                                                                       cTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGgcgg  >  1:4078079/1‑92 (MQ=255)

GTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGC  >  NC_000913/2416369‑2416548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGA  >  NC_000913/2416367‑2416550

GCGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCG                                                                                            >  SRR4302537.3462528/1‑94 (MQ=60)

 CGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGG                                                                                           <  SRR4302537.2629568/94‑1 (MQ=60)

 CGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGG                                                                                           <  SRR4302537.2739794/94‑1 (MQ=60)

  GTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGT                                                                                          <  SRR4302537.646073/94‑1 (MQ=60)

  GTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGT                                                                                          <  SRR4302537.4279174/94‑1 (MQ=60)

  GTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGT                                                                                          <  SRR4302537.333087/94‑1 (MQ=60)

  GTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGT                                                                                          >  SRR4302537.3249638/1‑94 (MQ=60)

   TTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTC                                                                                         <  SRR4302537.3927603/94‑1 (MQ=60)

   TTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTC                                                                                         <  SRR4302537.4419621/94‑1 (MQ=60)

      ACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACA                                                                                      >  SRR4302537.6402956/1‑94 (MQ=60)

       CCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACAT                                                                                     >  SRR4302537.3083516/1‑94 (MQ=60)

       CCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACAT                                                                                     <  SRR4302537.3806167/94‑1 (MQ=60)

         ATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCA                                                                                   >  SRR4302537.4351217/1‑94 (MQ=60)

           GCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATT                                                                                 <  SRR4302537.2206109/94‑1 (MQ=60)

            CCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTT                                                                                <  SRR4302537.5019422/94‑1 (MQ=60)

             CGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTA                                                                               >  SRR4302537.203959/1‑94 (MQ=60)

             CGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTA                                                                               <  SRR4302537.161614/94‑1 (MQ=60)

              GTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTAC                                                                              >  SRR4302537.3326325/1‑94 (MQ=60)

              GTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTAC                                                                              >  SRR4302537.6407197/1‑94 (MQ=60)

                TACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCC                                                                            >  SRR4302537.4680839/1‑94 (MQ=60)

                 ACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCAAGTTTGGTGATGGCTTCGGTCACATCATTTACCCA                                                                           >  SRR4302537.4510429/1‑94 (MQ=60)

                  CTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAC                                                                          >  SRR4302537.3598812/1‑94 (MQ=60)

                   TGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAAC                                                                         <  SRR4302537.4994817/94‑1 (MQ=60)

                    GGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACC                                                                        <  SRR4302537.1811026/94‑1 (MQ=60)

                       ACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCA                                                                     >  SRR4302537.6474567/1‑94 (MQ=60)

                        CCGCTTGGCGATCTGGTCGGTGTTAACCGTCATGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAG                                                                    >  SRR4302537.3113554/1‑94 (MQ=60)

                        CCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAG                                                                    <  SRR4302537.2476322/94‑1 (MQ=60)

                        CCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAG                                                                    <  SRR4302537.2022874/94‑1 (MQ=60)

                         CGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGC                                                                   >  SRR4302537.1766463/1‑94 (MQ=60)

                         CGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGC                                                                   <  SRR4302537.4464111/94‑1 (MQ=60)

                          GCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCG                                                                  <  SRR4302537.1570051/94‑1 (MQ=60)

                          CCTTGGCGGTCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCG                                                                  <  SRR4302537.2031109/94‑1 (MQ=60)

                           CTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGT                                                                 <  SRR4302537.5340968/94‑1 (MQ=60)

                           CTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGT                                                                 >  SRR4302537.3988739/1‑94 (MQ=60)

                             TGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCG                                                               >  SRR4302537.310856/1‑94 (MQ=60)

                                CGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTA                                                            >  SRR4302537.3895792/1‑94 (MQ=60)

                                CGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTA                                                            >  SRR4302537.2820758/1‑94 (MQ=60)

                                CGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTA                                                            <  SRR4302537.143717/94‑1 (MQ=60)

                                 GATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAA                                                           >  SRR4302537.3281778/1‑94 (MQ=60)

                                  cctgctgaaccgctcatccgatctattCCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGGCACATCATTTACCCAACCTCAGCGTCGTTAAT                                                          <  SRR4302537.5291459/67‑1 (MQ=60)

                                  ATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAAT                                                          <  SRR4302537.4828493/94‑1 (MQ=60)

                                   TCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATG                                                         >  SRR4302537.5422681/1‑94 (MQ=60)

                                      GGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCA                                                      <  SRR4302537.1021151/94‑1 (MQ=60)

                                      GGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCA                                                      <  SRR4302537.2822683/94‑1 (MQ=60)

                                        TCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAAC                                                    <  SRR4302537.2609295/94‑1 (MQ=60)

                                         CGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACG                                                   >  SRR4302537.6376678/1‑94 (MQ=60)

                                          GGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGC                                                  >  SRR4302537.3668408/1‑94 (MQ=60)

                                          GGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGC                                                  >  SRR4302537.2384753/1‑94 (MQ=60)

                                              TTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGG                                              <  SRR4302537.4282037/94‑1 (MQ=60)

                                               TAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTTATGGCTTCGGTTACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGT                                             >  SRR4302537.708903/1‑94 (MQ=60)

                                               TAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGT                                             <  SRR4302537.6287336/94‑1 (MQ=60)

                                                 ACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGT                                           >  SRR4302537.4479050/1‑94 (MQ=60)

                                                 ACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGT                                           >  SRR4302537.5365575/1‑94 (MQ=60)

                                                 ACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCGCGGTGG                                           >  SRR4302537.489121/1‑94 (MQ=60)

                                                  CCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTG                                          <  SRR4302537.67276/94‑1 (MQ=60)

                                                  CCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGCTAATGGAAACGCTCGGTGTG                                          >  SRR4302537.848976/1‑94 (MQ=60)

                                                   CGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGT                                         >  SRR4302537.2625342/1‑94 (MQ=60)

                                                     TCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGC                                       >  SRR4302537.4693539/1‑94 (MQ=60)

                                                      CAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCG                                      >  SRR4302537.4975788/1‑94 (MQ=60)

                                                      CAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCG                                      <  SRR4302537.3576536/94‑1 (MQ=60)

                                                      CAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCG                                      <  SRR4302537.4291850/94‑1 (MQ=60)

                                                       AGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGG                                     >  SRR4302537.3531606/1‑94 (MQ=60)

                                                       AGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGG                                     >  SRR4302537.4281206/1‑94 (MQ=60)

                                                       AGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGG                                     <  SRR4302537.3943963/94‑1 (MQ=60)

                                                        GGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGG                                    >  SRR4302537.2517158/1‑94 (MQ=60)

                                                        GGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGG                                    >  SRR4302537.1522565/1‑94 (MQ=60)

                                                        GGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGG                                    <  SRR4302537.2174035/94‑1 (MQ=60)

                                                        GGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGG                                    <  SRR4302537.1556286/94‑1 (MQ=60)

                                                           TACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTG                                 >  SRR4302537.1904096/1‑94 (MQ=60)

                                                            ACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGG                                >  SRR4302537.1283033/1‑94 (MQ=60)

                                                             CCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGA                               >  SRR4302537.3015072/1‑94 (MQ=60)

                                                              CGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGAC                              >  SRR4302537.766012/1‑94 (MQ=60)

                                                               GTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCTACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACT                             >  SRR4302537.6285338/1‑94 (MQ=60)

                                                               GTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACT                             >  SRR4302537.6040087/1‑94 (MQ=60)

                                                                 GCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTC                           >  SRR4302537.6163072/1‑94 (MQ=60)

                                                                  CTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCC                          >  SRR4302537.2239196/1‑94 (MQ=60)

                                                                  CTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCC                          <  SRR4302537.5902880/94‑1 (MQ=60)

                                                                   TGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCG                         <  SRR4302537.5215189/94‑1 (MQ=60)

                                                                      CGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAA                      >  SRR4302537.2519926/1‑94 (MQ=60)

                                                                       GTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAAC                     <  SRR4302537.1707165/94‑1 (MQ=60)

                                                                        TTCCAGTTTGGTGATGGCTTCGGTCACATCATTTAACCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACT                    >  SRR4302537.5091909/1‑94 (MQ=60)

                                                                         TCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTG                   >  SRR4302537.3779713/1‑94 (MQ=60)

                                                                         TCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTG                   <  SRR4302537.3682945/94‑1 (MQ=60)

                                                                          CCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGG                  >  SRR4302537.909197/1‑94 (MQ=60)

                                                                          CCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGG                  >  SRR4302537.101602/1‑94 (MQ=60)

                                                                          CCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGG                  <  SRR4302537.2200868/94‑1 (MQ=60)

                                                                             GTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTG               >  SRR4302537.297294/1‑94 (MQ=60)

                                                                             GTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTG               >  SRR4302537.3505121/1‑94 (MQ=60)

                                                                              TTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGA              <  SRR4302537.3977208/94‑1 (MQ=60)

                                                                               TTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAA             <  SRR4302537.581052/94‑1 (MQ=60)

                                                                                TGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAG            <  SRR4302537.4268852/94‑1 (MQ=60)

                                                                                TGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAG            <  SRR4302537.4229172/94‑1 (MQ=60)

                                                                                TGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAG            >  SRR4302537.763084/1‑94 (MQ=60)

                                                                                   TGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTG         <  SRR4302537.5862337/94‑1 (MQ=60)

                                                                                    GATGGCTTCGGTCACATCATTTACCCAACCTGAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGG        <  SRR4302537.796072/94‑1 (MQ=60)

                                                                                    GATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGG        >  SRR4302537.5706165/1‑94 (MQ=60)

                                                                                      TGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGC      >  SRR4302537.6483773/1‑94 (MQ=60)

                                                                                       GGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCG     <  SRR4302537.2477107/94‑1 (MQ=60)

                                                                                        GCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGG    >  SRR4302537.4106552/1‑94 (MQ=60)

                                                                                          TTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGA  >  SRR4302537.2131581/1‑94 (MQ=60)

GCGTTAACCATGCCGTTACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGA  >  NC_000913/2416367‑2416550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`