Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,416,514	A→G	R479G (AGG→GGG)	yfcC →	putative S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,416,514	0	A	G	99.1%	732.5 / ‑3.1	215	R479G (AGG→GGG)	yfcC	putative S‑transferase
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (100/113); total (102/113)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 227 total aligned reads displayed.

GGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGT  >  NC_000913/2416423‑2416604

ggTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCggg                                                                                          <  1:4492161/94‑1 (MQ=255)

ggTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCggg                                                                                          >  1:4827486/1‑94 (MQ=255)

  ttACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCggggg                                                                                        >  2:3633197/1‑93 (MQ=255)

  ttACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGt                                                                                        <  1:10161324/94‑1 (MQ=255)

  gttccGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGt                                                                                        <  2:8887962/91‑1 (MQ=255)

   tACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTg                                                                                       >  1:8399376/1‑94 (MQ=255)

    aCCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTgg                                                                                      <  1:1983083/94‑1 (MQ=255)

     ccGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTggc                                                                                     >  2:2662487/1‑93 (MQ=255)

     ccGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGa                                                                                     >  1:10299043/1‑94 (MQ=255)

      cGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGAc                                                                                    >  2:2846869/1‑94 (MQ=255)

      cGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGAc                                                                                    >  1:1152615/1‑94 (MQ=255)

      cGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGAc                                                                                    >  1:5412337/1‑94 (MQ=255)

          cTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTcc                                                                                >  2:2401822/1‑94 (MQ=255)

          cTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTcc                                                                                <  1:7040640/94‑1 (MQ=255)

           cggCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCg                                                                               <  2:2128709/93‑1 (MQ=255)

               gTTCCAGTTTTGTGATGGCTTCAGTCACCTCATTTCCCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAAc                                                                           <  2:3708206/94‑1 (MQ=255)

                 tCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTg                                                                         <  2:1024980/94‑1 (MQ=255)

                   cAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGc                                                                       >  2:8620703/1‑94 (MQ=255)

                   cAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGc                                                                       >  2:6192422/1‑94 (MQ=255)

                     gTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTg                                                                     >  2:24656/1‑94 (MQ=255)

                     gTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTg                                                                     <  2:3311031/94‑1 (MQ=255)

                       ttGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGaa                                                                   <  1:7512873/94‑1 (MQ=255)

                        tGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAg                                                                  <  1:6430477/94‑1 (MQ=255)

                        tGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAg                                                                  <  1:8828501/94‑1 (MQ=255)

                          gTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGt                                                                <  1:5192618/94‑1 (MQ=255)

                          gTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGt                                                                <  2:531915/94‑1 (MQ=255)

                            gATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTgg                                                              <  2:7309187/94‑1 (MQ=255)

                            gATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTgg                                                              <  2:5784525/94‑1 (MQ=255)

                             aTGGCTTCAGTCACCTCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTggg                                                             <  2:4149471/94‑1 (MQ=255)

                              tGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGgc                                                            >  1:3811775/1‑94 (MQ=255)

                               ggCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGgcg                                                           >  1:9928778/1‑94 (MQ=255)

                                gCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGgcgc                                                          <  2:6436092/94‑1 (MQ=255)

                                 cTTCAGTCACATCATTTACCCAACCGCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGcgcg                                                         <  2:6514688/94‑1 (MQ=255)

                                  ttCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGa                                                        >  1:3135474/1‑94 (MQ=255)

                                    taGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGAcc                                                      <  1:7916037/93‑1 (MQ=255)

                                    cAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGAcc                                                      <  2:5060348/94‑1 (MQ=255)

                                     aGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGAccc                                                     <  1:263361/94‑1 (MQ=255)

                                      gTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCt                                                    <  1:3274634/94‑1 (MQ=255)

                                       tCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTa                                                   <  2:9654920/94‑1 (MQ=255)

                                        cacaTCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTAc                                                  <  1:8179391/94‑1 (MQ=255)

                                         acaTCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACt                                                 >  1:1369932/1‑94 (MQ=255)

                                          catcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACtt                                                <  1:7845670/94‑1 (MQ=255)

                                           atcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTg                                               >  1:10581350/1‑94 (MQ=255)

                                            tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTgg                                              <  2:7085799/94‑1 (MQ=255)

                                            tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTgg                                              >  2:8361697/1‑94 (MQ=255)

                                             catTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGa                                             >  1:4043717/1‑94 (MQ=255)

                                             catTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGa                                             >  2:712997/1‑94 (MQ=255)

                                               tttACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGAct                                           >  1:6751939/1‑94 (MQ=255)

                                               tttACCCAACCTCAGCGTCGTGAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGAct                                           <  1:4692336/94‑1 (MQ=255)

                                                ttACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGActg                                          >  2:9480866/1‑94 (MQ=255)

                                                ttACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGActg                                          >  2:6734580/1‑94 (MQ=255)

                                                ttACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGActg                                          >  1:7787642/1‑94 (MQ=255)

                                                  aCCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGActgct                                        >  1:7135611/1‑94 (MQ=255)

                                                      aaCCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGttt                                    >  1:3371435/1‑94 (MQ=255)

                                                      aaCCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGttt                                    <  1:8918063/94‑1 (MQ=255)

                                                       aCCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTtta                                   >  1:10356195/1‑94 (MQ=255)

                                                       aCCTCAGCGTCGTTAATGGCAACGCTCGGTGGGTGCGGGGTGGACTGCCGTAACTGGCTGAAGGTGGGCGCGACCCTCCTTGGACTGCTGTtta                                   <  2:1878627/94‑1 (MQ=255)

                                                        ccTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttat                                  >  1:5961620/1‑94 (MQ=255)

                                                        acTCAGCGTTGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttat                                  <  1:8259652/93‑1 (MQ=255)

                                                         cTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttatt                                 <  2:7610473/94‑1 (MQ=255)

                                                           cAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTtattat                               <  1:2642848/94‑1 (MQ=255)

                                                            aGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATg                              >  2:3094253/1‑94 (MQ=255)

                                                             gCGTCGTTAATGGCAACGCCCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGt                             >  1:5707791/1‑94 (MQ=255)

                                                              cgtcgtTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTc                            >  2:2562790/1‑94 (MQ=255)

                                                                tcgtTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAGTTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCa                          <  1:8308383/94‑1 (MQ=255)

                                                                tcgtTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCa                          <  2:4366219/94‑1 (MQ=255)

                                                                tcgtTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCa                          <  1:2052366/94‑1 (MQ=255)

                                                                tcgtTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCa                          <  2:4516432/94‑1 (MQ=255)

                                                                tcgtTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCa                          >  2:235875/1‑94 (MQ=255)

                                                                 cgtTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAg                         <  1:2133163/94‑1 (MQ=255)

                                                                 cgtTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAg                         <  2:6618074/94‑1 (MQ=255)

                                                                  gtTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGc                        <  1:1794317/94‑1 (MQ=255)

                                                                  gtTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGc                        <  1:2846869/94‑1 (MQ=255)

                                                                  gtTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGc                        >  1:5838029/1‑94 (MQ=255)

                                                                   ttAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGcg                       >  2:8117987/1‑94 (MQ=255)

                                                                   ttAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGcg                       <  1:1307090/94‑1 (MQ=255)

                                                                    tAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGcgt                      >  2:9343787/1‑94 (MQ=255)

                                                                      aTGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGcgtcg                    >  1:4346259/1‑94 (MQ=255)

                                                                       tGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGcgtcgt                   <  1:5944042/94‑1 (MQ=255)

                                                                        ggCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTa                  >  1:867976/1‑94 (MQ=255)

                                                                         gCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAg                 <  2:1207655/94‑1 (MQ=255)

                                                                          cAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGt                <  2:1043994/94‑1 (MQ=255)

                                                                             cGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGAt             >  2:4368607/1‑94 (MQ=255)

                                                                             cGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGAt             <  1:4250610/94‑1 (MQ=255)

                                                                             cGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGAt             <  1:5665557/94‑1 (MQ=255)

                                                                              gCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATc            <  2:1410234/94‑1 (MQ=255)

                                                                               cTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGGGCGACCCTACTTGGACTGCTGTTTATTATGTCCCGCGTCGTAGTGAtag           >  2:7883228/1‑92 (MQ=255)

                                                                               cTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCg           >  1:4137801/1‑94 (MQ=255)

                                                                                tCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCgg          >  2:8738702/1‑94 (MQ=255)

                                                                                 cGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGgc         >  2:9195990/1‑94 (MQ=255)

                                                                                  ggTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGgcg        >  1:2671170/1‑94 (MQ=255)

                                                                                  ggTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGCGATCGgcg        >  1:5474176/1‑94 (MQ=255)

                                                                                   gtgtgtGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGgcgc       >  2:6131229/1‑94 (MQ=255)

                                                                                      tgtgCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCa    <  1:2277818/94‑1 (MQ=255)

                                                                                      tgtgCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCa    <  2:5882148/94‑1 (MQ=255)

                                                                                       gtgCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAg   <  1:5030675/94‑1 (MQ=255)

                                                                                       gtgCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAg   >  2:5235486/1‑94 (MQ=255)

                                                                                       gtgCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAg   >  1:9596379/1‑94 (MQ=255)

                                                                                       gtgCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAg   <  1:6902506/94‑1 (MQ=255)

                                                                                        tgCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGt  >  2:1151847/1‑94 (MQ=255)

GGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGT  >  NC_000913/2416423‑2416604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGA  >  NC_000913/2416421‑2416607

CAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCG                                                                                               <  SRR4302545.4461000/94‑1 (MQ=60)

 AGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGG                                                                                              >  SRR4302545.237861/1‑94 (MQ=60)

 AGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGG                                                                                              <  SRR4302545.126793/94‑1 (MQ=60)

  GGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGG                                                                                             <  SRR4302545.4606958/94‑1 (MQ=60)

  GGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGG                                                                                             >  SRR4302545.4950688/1‑94 (MQ=60)

   GTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGG                                                                                            >  SRR4302545.806042/1‑94 (MQ=60)

    TTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGT                                                                                           <  SRR4302545.10461775/94‑1 (MQ=60)

    TTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGT                                                                                           <  SRR4302545.5636576/94‑1 (MQ=60)

    TTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGT                                                                                           <  SRR4302545.6064201/94‑1 (MQ=60)

    TTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTAGGTGTGTGCGGGGT                                                                                           >  SRR4302545.1330669/1‑94 (MQ=60)

     TACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTG                                                                                          >  SRR4302545.8642454/1‑94 (MQ=60)

     TACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTG                                                                                          >  SRR4302545.10051480/1‑94 (MQ=60)

      ACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGG                                                                                         <  SRR4302545.2032187/94‑1 (MQ=60)

       CCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGA                                                                                        >  SRR4302545.10602775/1‑94 (MQ=60)

        CGTGCTGGCGTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCAAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGAC                                                                                       >  SRR4302545.5553627/1‑94 (MQ=60)

        CGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGAC                                                                                       >  SRR4302545.5553626/1‑94 (MQ=60)

        CGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGAC                                                                                       >  SRR4302545.1177902/1‑94 (MQ=60)

         GTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACT                                                                                      >  SRR4302545.2723449/1‑94 (MQ=60)

           GCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTC                                                                                    >  SRR4302545.3528831/1‑94 (MQ=60)

           GCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTC                                                                                    <  SRR4302545.6881988/94‑1 (MQ=60)

            CTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCC                                                                                   <  SRR4302545.6262352/94‑1 (MQ=60)

            CTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCC                                                                                   <  SRR4302545.7235120/94‑1 (MQ=60)

            CTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCA                                                                                   <  SRR4302545.6002808/94‑1 (MQ=60)

              GGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGT                                                                                 >  SRR4302545.1924564/1‑94 (MQ=60)

               GCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTA                                                                                <  SRR4302545.3689519/94‑1 (MQ=60)

                CGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGGGGACTTCCGTAA                                                                               >  SRR4302545.2255293/1‑94 (MQ=60)

                 GTTCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAAC                                                                              <  SRR4302545.5494780/94‑1 (MQ=60)

                 GTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAAC                                                                              <  SRR4302545.4624183/94‑1 (MQ=60)

                  TTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACT                                                                             <  SRR4302545.3439544/94‑1 (MQ=60)

                   TCCAGTTTGGTGATGGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTG                                                                            <  SRR4302545.4369658/94‑1 (MQ=60)

                    CCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGG                                                                           >  SRR4302545.9170044/1‑94 (MQ=60)

                      AGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCT                                                                         <  SRR4302545.1142945/94‑1 (MQ=60)

                        TTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGA                                                                       >  SRR4302545.8031362/1‑94 (MQ=60)

                         TTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAA                                                                      <  SRR4302545.7725426/94‑1 (MQ=60)

                          TGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAG                                                                     <  SRR4302545.6606270/94‑1 (MQ=60)

                          TGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAG                                                                     <  SRR4302545.9084874/94‑1 (MQ=60)

                          TGGTGATGGCGTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCGCTTTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAG                                                                     <  SRR4302545.4709510/94‑1 (MQ=60)

                            GTGATGGCTTCAGTCACATCATTTACCTAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGT                                                                   <  SRR4302545.2092496/94‑1 (MQ=60)

                            GTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGT                                                                   <  SRR4302545.5326341/94‑1 (MQ=60)

                               ATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGG                                                                >  SRR4302545.4314886/1‑94 (MQ=60)

                                TGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGC                                                               >  SRR4302545.3906520/1‑94 (MQ=60)

                                 GGCTTCGGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCG                                                              >  SRR4302545.10223636/1‑94 (MQ=60)

                                   CTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCG                                                            >  SRR4302545.2543158/1‑94 (MQ=60)

                                    TTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGA                                                           >  SRR4302545.3212750/1‑94 (MQ=60)

                                     TCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGAC                                                          <  SRR4302545.5004258/94‑1 (MQ=60)

                                     TCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGAC                                                          <  SRR4302545.320056/94‑1 (MQ=60)

                                     TCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGAC                                                          >  SRR4302545.10558484/1‑94 (MQ=60)

                                      TAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACC                                                         <  SRR4302545.8140776/94‑1 (MQ=60)

                                       AGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCC                                                        >  SRR4302545.9850939/1‑94 (MQ=60)

                                       AGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCC                                                        <  SRR4302545.268797/94‑1 (MQ=60)

                                       AGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCC                                                        >  SRR4302545.8440821/1‑94 (MQ=60)

                                        GTCACATCATTTACCCACCCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCT                                                       <  SRR4302545.4201449/94‑1 (MQ=60)

                                        GTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCT                                                       <  SRR4302545.8151802/94‑1 (MQ=60)

                                        GTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCT                                                       <  SRR4302545.3354895/94‑1 (MQ=60)

                                          CACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTAC                                                     <  SRR4302545.8415994/94‑1 (MQ=60)

                                          CACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTAC                                                     <  SRR4302545.9001501/94‑1 (MQ=60)

                                           ACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACT                                                    >  SRR4302545.1400597/1‑94 (MQ=60)

                                           ACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACT                                                    <  SRR4302545.7125621/94‑1 (MQ=60)

                                            CATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTT                                                   <  SRR4302545.8068125/94‑1 (MQ=60)

                                             ATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTG                                                  <  SRR4302545.4404768/94‑1 (MQ=60)

                                             ATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTG                                                  >  SRR4302545.10893024/1‑94 (MQ=60)

                                               CATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGA                                                >  SRR4302545.4144967/1‑94 (MQ=60)

                                                ATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGAC                                               <  SRR4302545.1666710/94‑1 (MQ=60)

                                                ATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGAC                                               <  SRR4302545.4875189/94‑1 (MQ=60)

                                                 TTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACT                                              >  SRR4302545.6937093/1‑94 (MQ=60)

                                                 TTTACCCAACCTCAGCGTCGTGAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACT                                              <  SRR4302545.4811919/94‑1 (MQ=60)

                                                  TTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTG                                             <  SRR4302545.6111486/94‑1 (MQ=60)

                                                  TTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTG                                             >  SRR4302545.8008086/1‑94 (MQ=60)

                                                    ACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTCACTGGCTGAAGGTGGGCGCGACACTACTTGGACTGCT                                           >  SRR4302545.7333518/1‑94 (MQ=60)

                                                    ACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCT                                           >  SRR4302545.7333516/1‑94 (MQ=60)

                                                      CCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGT                                         <  SRR4302545.10649918/94‑1 (MQ=60)

                                                        AACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTT                                       <  SRR4302545.9177333/94‑1 (MQ=60)

                                                        AACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTT                                       >  SRR4302545.3453648/1‑94 (MQ=60)

                                                        AACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGAGCGACCCTACTTGGACTGCTGTTT                                       >  SRR4302545.1347542/1‑94 (MQ=60)

                                                         ACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTA                                      >  SRR4302545.10661458/1‑94 (MQ=60)

                                                          CCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTAT                                     >  SRR4302545.6122436/1‑94 (MQ=60)

                                                          ACTCAGCGTTGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTAT                                     <  SRR4302545.8498704/94‑1 (MQ=60)

                                                           CTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATT                                    >  SRR4302545.8386354/1‑94 (MQ=60)

                                                             CAGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTAT                                  <  SRR4302545.2706452/94‑1 (MQ=60)

                                                              AGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATG                                 >  SRR4302545.4006988/1‑94 (MQ=60)

                                                              AGCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATG                                 <  SRR4302545.7744855/94‑1 (MQ=60)

                                                               GCGTGGTTAAAGGCAACGCTAGGTGAGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGGTCCTACTTGGACTGCTGTTTATTATGT                                <  SRR4302545.8698779/94‑1 (MQ=60)

                                                               GCGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGT                                <  SRR4302545.2581714/94‑1 (MQ=60)

                                                               GCGTCGTTAATGGCAACGCCCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGT                                >  SRR4302545.5860741/1‑94 (MQ=60)

                                                                CGTCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTC                               <  SRR4302545.8706571/94‑1 (MQ=60)

                                                                  TCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAGTTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCA                             <  SRR4302545.8549139/94‑1 (MQ=60)

                                                                  TCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCA                             <  SRR4302545.5303304/94‑1 (MQ=60)

                                                                  TCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCA                             <  SRR4302545.8549137/94‑1 (MQ=60)

                                                                  TCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCA                             <  SRR4302545.5566054/94‑1 (MQ=60)

                                                                  TCGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCA                             <  SRR4302545.2102931/94‑1 (MQ=60)

                                                                   GGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGTTGGGCGCGCCCCTACCTGGACTGCTGTTTATTATGTCCAG                            <  SRR4302545.5949443/94‑1 (MQ=60)

                                                                   CGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAG                            <  SRR4302545.2185469/94‑1 (MQ=60)

                                                                   CGTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAG                            >  SRR4302545.2174476/1‑94 (MQ=60)

                                                                    GTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGC                           <  SRR4302545.2915718/94‑1 (MQ=60)

                                                                    GTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGC                           >  SRR4302545.5995198/1‑94 (MQ=60)

                                                                    GTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGC                           <  SRR4302545.1837634/94‑1 (MQ=60)

                                                                    GTTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTAGACTGCTGTTTATTATGTCCAGC                           >  SRR4302545.5995196/1‑94 (MQ=60)

                                                                     TTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCCGCG                          >  SRR4302545.6998781/1‑94 (MQ=60)

                                                                     TTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCG                          >  SRR4302545.2705919/1‑94 (MQ=60)

                                                                     TTAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCG                          <  SRR4302545.1336096/94‑1 (MQ=60)

                                                                      TAATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGT                         >  SRR4302545.7433938/1‑94 (MQ=60)

                                                                       AATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTC                        <  SRR4302545.4572438/94‑1 (MQ=60)

                                                                        ATGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCG                       >  SRR4302545.4455971/1‑94 (MQ=60)

                                                                         TGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGT                      <  SRR4302545.7025784/94‑1 (MQ=60)

                                                                         TGGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGT                      <  SRR4302545.6104409/94‑1 (MQ=60)

                                                                          GGCACCGCTCGGTGTGTGGGGGGTGGATTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTA                     <  SRR4302545.7297781/94‑1 (MQ=60)

                                                                          GGCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTA                     >  SRR4302545.887447/1‑94 (MQ=60)

                                                                           GCAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAG                    >  SRR4302545.110164/1‑94 (MQ=60)

                                                                            CAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGGGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGT                   >  SRR4302545.2819715/1‑94 (MQ=60)

                                                                            CAACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGT                   >  SRR4302545.1994252/1‑94 (MQ=60)

                                                                             AACGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGTCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGCTTATTATGTCCAGCGCCGTAGTG                  >  SRR4302545.4842178/1‑94 (MQ=60)

                                                                               CGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGAT                <  SRR4302545.4357071/94‑1 (MQ=60)

                                                                               CGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGAT                >  SRR4302545.286973/1‑94 (MQ=60)

                                                                               CGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGAT                <  SRR4302545.5816716/94‑1 (MQ=60)

                                                                               CGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGAT                <  SRR4302545.5816717/94‑1 (MQ=60)

                                                                               CGCTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGAT                <  SRR4302545.9644540/94‑1 (MQ=60)

                                                                                 CTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCG              >  SRR4302545.4241217/1‑94 (MQ=60)

                                                                                 CTCGGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGCGCGCGACCCTACTTGGACTGCTGGTTATTATGTCCAGCGTCGTAGTGATCG              <  SRR4302545.10850468/94‑1 (MQ=60)

                                                                                    GGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCG           >  SRR4302545.2735523/1‑94 (MQ=60)

                                                                                    GGTGTGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGCGATCGGCG           >  SRR4302545.5617907/1‑94 (MQ=60)

                                                                                        TGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCA       <  SRR4302545.2333228/94‑1 (MQ=60)

                                                                                        TGTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCA       >  SRR4302545.856627/1‑94 (MQ=60)

                                                                                         GTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAG      >  SRR4302545.9879152/1‑94 (MQ=60)

                                                                                         GTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAG      <  SRR4302545.7092630/94‑1 (MQ=60)

                                                                                         GTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAG      <  SRR4302545.5158220/94‑1 (MQ=60)

                                                                                         GTGCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAG      <  SRR4302545.3307307/94‑1 (MQ=60)

                                                                                           GCGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTT    >  SRR4302545.10522885/1‑94 (MQ=60)

                                                                                            CGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTG   <  SRR4302545.4839011/94‑1 (MQ=60)

                                                                                            CGGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTG   <  SRR4302545.2554195/94‑1 (MQ=60)

                                                                                             GGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGA  >  SRR4302545.7696021/1‑94 (MQ=60)

                                                                                             GGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGGTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGA  >  SRR4302545.5378200/1‑94 (MQ=60)

CAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGA  >  NC_000913/2416421‑2416607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`