Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,457,111 T→C V23V (GTA→GTG)  b2341 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,457,1110TC99.5% 672.0 / ‑5.6 194V23V (GTA→GTG) b2341hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (85/108);  total (86/108)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

TCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATT  >  NC_000913/2457021‑2457200
                                                                                          |                                                                                         
tCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCAc                                                                                         >  2:1005713/1‑93 (MQ=255)
   cAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCAGCACGtg                                                                                      >  1:2398758/1‑92 (MQ=255)
     aCTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGa                                                                                    <  2:4521056/93‑1 (MQ=255)
      ctctTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGAt                                                                                   >  2:360811/1‑93 (MQ=255)
           tGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGa                                                                              <  2:3162862/93‑1 (MQ=255)
           tGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGa                                                                              <  1:2239005/93‑1 (MQ=255)
            gTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGAt                                                                             >  2:3552221/1‑93 (MQ=255)
              tttCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATaa                                                                           <  1:3205331/93‑1 (MQ=255)
              tttCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATaa                                                                           <  1:578564/93‑1 (MQ=255)
               ttCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAAc                                                                          <  2:6383002/93‑1 (MQ=255)
                tCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACg                                                                         >  2:1180327/1‑93 (MQ=255)
                 cACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACgg                                                                        <  1:2983758/93‑1 (MQ=255)
                  aCGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGc                                                                       <  2:6094779/93‑1 (MQ=255)
                   cGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCa                                                                      <  2:4695216/93‑1 (MQ=255)
                   cGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCa                                                                      >  1:2206514/1‑93 (MQ=255)
                    ggAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCaa                                                                     >  1:1242440/1‑93 (MQ=255)
                       gTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgt                                                                  <  2:5899356/93‑1 (MQ=255)
                       gTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgt                                                                  >  1:5162208/1‑93 (MQ=255)
                       gTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgt                                                                  >  2:623659/1‑93 (MQ=255)
                        ttGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgtt                                                                 <  2:4080532/93‑1 (MQ=255)
                          tcTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt                                                               <  1:1751101/92‑1 (MQ=255)
                          gCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt                                                               <  2:306233/93‑1 (MQ=255)
                          gCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt                                                               >  2:5108234/1‑93 (MQ=255)
                          gCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt                                                               >  1:4204971/1‑93 (MQ=255)
                          gCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAAtgttgt                                                               <  2:4929707/93‑1 (MQ=255)
                           cTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTc                                                              >  1:4747028/1‑93 (MQ=255)
                            ttAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGTGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTcc                                                             >  1:797932/1‑93 (MQ=255)
                            ttAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTcc                                                             <  2:2005559/93‑1 (MQ=255)
                            ttAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTcc                                                             <  1:4490481/93‑1 (MQ=255)
                             taataaTGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCa                                                            <  1:1446169/93‑1 (MQ=255)
                             taataaTGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCa                                                            <  1:2771377/93‑1 (MQ=255)
                               ataatGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGa                                                          >  1:1094160/1‑93 (MQ=255)
                               ataatGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGa                                                          <  2:5154447/93‑1 (MQ=255)
                                taatGGCGCGCGCCTGCGAGGCAACCTGCGCCTTCACGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGAc                                                         <  1:753851/93‑1 (MQ=255)
                                taatGGCGCGCGCCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGAc                                                         <  2:1322229/93‑1 (MQ=255)
                                        cgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTa                                                 <  1:4887603/93‑1 (MQ=255)
                                        cgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTa                                                 <  2:1341049/93‑1 (MQ=255)
                                         gcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTaa                                                <  2:4143158/93‑1 (MQ=255)
                                          cACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAg                                               <  2:2278871/93‑1 (MQ=255)
                                          cACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAg                                               >  2:1941379/1‑93 (MQ=255)
                                           aCCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAgg                                              >  2:898799/1‑93 (MQ=255)
                                           aCCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAgg                                              >  1:6273156/1‑93 (MQ=255)
                                           aCCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAgg                                              >  2:1443459/1‑93 (MQ=255)
                                            ccTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAggg                                             <  2:5735558/93‑1 (MQ=255)
                                            ccTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAggg                                             >  2:6466875/1‑93 (MQ=255)
                                            ccTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAggg                                             >  2:282106/1‑93 (MQ=255)
                                             cTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGt                                            >  1:5106697/1‑93 (MQ=255)
                                             cTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGt                                            <  2:4200509/93‑1 (MQ=255)
                                              tGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTa                                           >  2:5632007/1‑93 (MQ=255)
                                              tGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTa                                           >  2:3958128/1‑93 (MQ=255)
                                              ggCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTa                                           <  2:367432/92‑1 (MQ=255)
                                               gCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTaa                                          <  1:3296345/93‑1 (MQ=255)
                                               gCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTaa                                          <  1:3287881/93‑1 (MQ=255)
                                                 gAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAAc                                        >  1:5369353/1‑93 (MQ=255)
                                                 gAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAAc                                        >  1:5199739/1‑93 (MQ=255)
                                                  aGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACg                                       >  1:4565929/1‑93 (MQ=255)
                                                  aGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACg                                       >  2:3214536/1‑93 (MQ=255)
                                                   ggCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGc                                      <  2:3715943/93‑1 (MQ=255)
                                                   ggCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGc                                      <  2:3688964/93‑1 (MQ=255)
                                                   ggCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGc                                      >  1:587955/1‑93 (MQ=255)
                                                    gCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCt                                     >  1:3969914/1‑93 (MQ=255)
                                                      aaaCTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGCTAACGGCAATGTTGTCCAGACGAACATTCAGGGTAAACGCTGa                                   >  2:725257/1‑93 (MQ=255)
                                                       aaCTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGAt                                  <  1:3662047/93‑1 (MQ=255)
                                                       aaCTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGAt                                  <  2:3443621/93‑1 (MQ=255)
                                                       aaCTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGAt                                  >  2:1277977/1‑93 (MQ=255)
                                                          tCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTc                               <  2:2510656/93‑1 (MQ=255)
                                                          tCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTc                               >  2:2164314/1‑93 (MQ=255)
                                                           ccGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCa                              >  1:5774561/1‑93 (MQ=255)
                                                           ccGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCa                              <  2:3888389/93‑1 (MQ=255)
                                                             gCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCAtt                            <  1:2608628/93‑1 (MQ=255)
                                                             gCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCAtt                            <  1:5490833/93‑1 (MQ=255)
                                                             ctcTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCAtt                            <  1:1305918/91‑1 (MQ=255)
                                                              ccTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCAttt                           <  1:5297364/93‑1 (MQ=255)
                                                                ttCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTcc                         >  1:3078851/1‑93 (MQ=255)
                                                                ttCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTcc                         >  2:5757652/1‑93 (MQ=255)
                                                                ttCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTcc                         >  1:6089003/1‑93 (MQ=255)
                                                                ttCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTcc                         <  1:10232/93‑1 (MQ=255)
                                                                  cAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCACTTCCat                       <  2:5369043/93‑1 (MQ=255)
                                                                   aGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCatt                      >  1:931490/1‑93 (MQ=255)
                                                                    gggTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCatta                     <  2:2609165/93‑1 (MQ=255)
                                                                    gggTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCatta                     >  2:1683820/1‑93 (MQ=255)
                                                                    gggTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCatta                     >  2:4419744/1‑93 (MQ=255)
                                                                      gTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCattatt                   <  2:976277/93‑1 (MQ=255)
                                                                      gTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCattatt                   >  2:1599667/1‑93 (MQ=255)
                                                                       tATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTc                  <  2:1494954/93‑1 (MQ=255)
                                                                         ttCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCg                <  2:5720385/93‑1 (MQ=255)
                                                                            aTTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCt             >  2:867308/1‑93 (MQ=255)
                                                                             ttttCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTc            >  2:6267649/1‑93 (MQ=255)
                                                                               ttCTCCCCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCa          <  1:4950601/93‑1 (MQ=255)
                                                                               ttCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCa          <  2:1664034/93‑1 (MQ=255)
                                                                               ttCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCa          >  1:4179525/1‑93 (MQ=255)
                                                                                tctcACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAg         >  2:526418/1‑93 (MQ=255)
                                                                                tctcACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAg         >  1:2555765/1‑93 (MQ=255)
                                                                                  tcACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGaa       <  1:2063134/93‑1 (MQ=255)
                                                                                  tcACCCGGCAAGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGaa       <  1:4278257/93‑1 (MQ=255)
                                                                                   cACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAAc      <  2:5098650/93‑1 (MQ=255)
                                                                                    aCCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAAcc     >  1:2840835/1‑93 (MQ=255)
                                                                                    aCCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGCCATTTCCATTATTCCGCCTCCAGAAcc     >  1:6315270/1‑93 (MQ=255)
                                                                                      ccGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCAt   <  2:5797621/93‑1 (MQ=255)
                                                                                      ccGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCAt   <  2:1804893/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATT  >  NC_000913/2457021‑2457200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCG  >  NC_000913/2457018‑2457204
                                                                                             |                                                                                             
GCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGC                                                                                               <  SRR4302537.43008/94‑1 (MQ=60)
 CCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCA                                                                                              >  SRR4302537.4442926/1‑94 (MQ=60)
 CCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCA                                                                                              >  SRR4302537.61732/1‑94 (MQ=60)
  CTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTAC                                                                                             >  SRR4302537.5018285/1‑94 (MQ=60)
  CTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCAC                                                                                             <  SRR4302537.2074713/94‑1 (MQ=60)
        ACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGAT                                                                                       >  SRR4302537.6014728/1‑94 (MQ=60)
           CTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGT                                                                                    <  SRR4302537.5698360/94‑1 (MQ=60)
            TTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTG                                                                                   <  SRR4302537.2544798/94‑1 (MQ=60)
              TGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGAT                                                                                 <  SRR4302537.2254792/94‑1 (MQ=60)
              TGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGAT                                                                                 <  SRR4302537.137607/94‑1 (MQ=60)
                TTTTCACGGAGTTGCTTAATAATGGCGCGCGCCTGCGAGGCAAGCTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAA                                                                               <  SRR4302537.4224563/94‑1 (MQ=60)
                 TTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAAC                                                                              <  SRR4302537.3227864/94‑1 (MQ=60)
                 TTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAAC                                                                              <  SRR4302537.3312178/94‑1 (MQ=60)
                 TTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAAC                                                                              <  SRR4302537.582525/94‑1 (MQ=60)
                    CACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGC                                                                           <  SRR4302537.3004612/94‑1 (MQ=60)
                     ACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCA                                                                          >  SRR4302537.2222071/1‑94 (MQ=60)
                      CGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAA                                                                         >  SRR4302537.1251099/1‑94 (MQ=60)
                         AGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGT                                                                      >  SRR4302537.5197792/1‑94 (MQ=60)
                           TTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTG                                                                    <  SRR4302537.928172/94‑1 (MQ=60)
                            TGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGT                                                                   >  SRR4302537.4234319/1‑94 (MQ=60)
                             TCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTC                                                                  <  SRR4302537.1763304/94‑1 (MQ=60)
                             GCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTC                                                                  >  SRR4302537.4779824/1‑94 (MQ=60)
                             GCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTC                                                                  <  SRR4302537.6350496/94‑1 (MQ=60)
                              CTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGTGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCC                                                                 >  SRR4302537.803410/1‑94 (MQ=60)
                               TTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCA                                                                <  SRR4302537.4521542/94‑1 (MQ=60)
                               TTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCA                                                                <  SRR4302537.492475/94‑1 (MQ=60)
                                TAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAG                                                               <  SRR4302537.1456245/94‑1 (MQ=60)
                                TAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAG                                                               <  SRR4302537.2790746/94‑1 (MQ=60)
                                 AATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGA                                                              >  SRR4302537.1101718/1‑94 (MQ=60)
                                  ATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTACAGAC                                                             >  SRR4302537.2358958/1‑94 (MQ=60)
                                   TAATGGCGCGCGCCTGCGAGGCAACCTGCGCCTTCACGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACG                                                            <  SRR4302537.758991/94‑1 (MQ=60)
                                   TAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACG                                                            <  SRR4302537.962095/94‑1 (MQ=60)
                                      TGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAAC                                                         <  SRR4302537.1257207/94‑1 (MQ=60)
                                        GCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACAT                                                       >  SRR4302537.1817432/1‑94 (MQ=60)
                                         CGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATT                                                      <  SRR4302537.4252741/94‑1 (MQ=60)
                                           CGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAA                                                    <  SRR4302537.4921351/94‑1 (MQ=60)
                                            GCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAG                                                   <  SRR4302537.2982878/94‑1 (MQ=60)
                                             CACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGG                                                  >  SRR4302537.5224682/1‑94 (MQ=60)
                                             CACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGG                                                  <  SRR4302537.329922/94‑1 (MQ=60)
                                             CACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGG                                                  >  SRR4302537.6316202/1‑94 (MQ=60)
                                               CCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGT                                                >  SRR4302537.5141888/1‑94 (MQ=60)
                                               CCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGT                                                <  SRR4302537.1701182/94‑1 (MQ=60)
                                                CTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTA                                               <  SRR4302537.5167819/94‑1 (MQ=60)
                                                 TGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAA                                              >  SRR4302537.5830941/1‑94 (MQ=60)
                                                  GCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAA                                             <  SRR4302537.3319646/94‑1 (MQ=60)
                                                  GCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAA                                             <  SRR4302537.3311119/94‑1 (MQ=60)
                                                   CGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAAC                                            >  SRR4302537.5406087/1‑94 (MQ=60)
                                                   CGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAAC                                            >  SRR4302537.5235509/1‑94 (MQ=60)
                                                    GAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACG                                           >  SRR4302537.4597534/1‑94 (MQ=60)
                                                     AGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGC                                          >  SRR4302537.591968/1‑94 (MQ=60)
                                                      GGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCT                                         >  SRR4302537.3997694/1‑94 (MQ=60)
                                                        CAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGA                                       <  SRR4302537.2681998/94‑1 (MQ=60)
                                                         AAACTCCGCCTTCAGGGTATGCATTTTCTCACCCGGCACGTAGATGATGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGAT                                      <  SRR4302537.6203030/94‑1 (MQ=60)
                                                          AACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATG                                     <  SRR4302537.3687822/94‑1 (MQ=60)
                                                            CTCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTC                                   >  SRR4302537.800072/1‑94 (MQ=60)
                                                             TCCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCA                                  >  SRR4302537.5814141/1‑94 (MQ=60)
                                                              CCGCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCAT                                 >  SRR4302537.2574667/1‑94 (MQ=60)
                                                                ctCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTT                               <  SRR4302537.1314993/92‑1 (MQ=60)
                                                                GCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTT                               <  SRR4302537.5528321/94‑1 (MQ=60)
                                                                GCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTT                               >  SRR4302537.1174116/1‑94 (MQ=60)
                                                                GCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTT                               <  SRR4302537.2626873/94‑1 (MQ=60)
                                                                GCCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTT                               <  SRR4302537.6275237/94‑1 (MQ=60)
                                                                 CCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTC                              <  SRR4302537.5333754/94‑1 (MQ=60)
                                                                 CCTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTC                              <  SRR4302537.5822163/94‑1 (MQ=60)
                                                                  CTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCC                             >  SRR4302537.6130834/1‑94 (MQ=60)
                                                                  CTTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCC                             >  SRR4302537.3100297/1‑94 (MQ=60)
                                                                   TTCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCA                            <  SRR4302537.10308/94‑1 (MQ=60)
                                                                    TCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCATACGAACATTAAGGGTAAACGCTGATGTCATTTCCAT                           <  SRR4302537.4565684/94‑1 (MQ=60)
                                                                    TCAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCAT                           <  SRR4302537.691131/94‑1 (MQ=60)
                                                                     CAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATT                          >  SRR4302537.937914/1‑94 (MQ=60)
                                                                     CAGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATT                          <  SRR4302537.5370936/94‑1 (MQ=60)
                                                                      AGGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTA                         >  SRR4302537.4394994/1‑94 (MQ=60)
                                                                       GGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTAT                        <  SRR4302537.2239061/94‑1 (MQ=60)
                                                                       GGGTATTCATTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTAT                        <  SRR4302537.3553421/94‑1 (MQ=60)
                                                                                TTTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCC               <  SRR4302537.3945331/94‑1 (MQ=60)
                                                                                 TTTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCA              >  SRR4302537.4208699/1‑94 (MQ=60)
                                                                                  TTCTCCCCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAG             <  SRR4302537.4984744/94‑1 (MQ=60)
                                                                                  TTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAG             >  SRR4302537.2573681/1‑94 (MQ=60)
                                                                                  TTCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAG             >  SRR4302537.5308148/1‑94 (MQ=60)
                                                                                   TCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGA            <  SRR4302537.3107436/94‑1 (MQ=60)
                                                                                   TCTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGA            <  SRR4302537.4646193/94‑1 (MQ=60)
                                                                                    CTCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAA           <  SRR4302537.3033734/94‑1 (MQ=60)
                                                                                     TCACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAAC          <  SRR4302537.2077622/94‑1 (MQ=60)
                                                                                     TCACCCGGCAAGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAAC          <  SRR4302537.4307992/94‑1 (MQ=60)
                                                                                      CACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACC         >  SRR4302537.2860716/1‑94 (MQ=60)
                                                                                      CACCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGCCATTTCCATTATTCCGCCTCCAGAACC         >  SRR4302537.6358493/1‑94 (MQ=60)
                                                                                        CCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCAT       <  SRR4302537.4788035/94‑1 (MQ=60)
                                                                                        CCCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCAT       >  SRR4302537.2175365/1‑94 (MQ=60)
                                                                                         CCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATT      >  SRR4302537.416802/1‑94 (MQ=60)
                                                                                         CCGGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATT      <  SRR4302537.2828960/94‑1 (MQ=60)
                                                                                           GGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGC    <  SRR4302537.5061055/94‑1 (MQ=60)
                                                                                           GGCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGC    <  SRR4302537.119901/94‑1 (MQ=60)
                                                                                            GCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCC   >  SRR4302537.159294/1‑94 (MQ=60)
                                                                                            GCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCC   >  SRR4302537.2138276/1‑94 (MQ=60)
                                                                                            GCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCC   <  SRR4302537.3366902/94‑1 (MQ=60)
                                                                                            GCACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCC   <  SRR4302537.2740158/94‑1 (MQ=60)
                                                                                             CACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCG  >  SRR4302537.2212244/1‑94 (MQ=60)
                                                                                             CACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCG  >  SRR4302537.3326316/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCG  >  NC_000913/2457018‑2457204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑