Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,457,232 T→C T420A (ACC→GCC)  b2342 ← putative acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,457,2320TC98.2% 940.7 / ‑3.9 280T420A (ACC→GCC) b2342putative acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base C (132/143);  total (136/144)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

CAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTA  >  NC_000913/2457141‑2457322
                                                                                           |                                                                                          
cAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCaa                                                                                          >  2:7722312/1‑94 (MQ=255)
    cGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTaa                                                                                      <  1:7464496/94‑1 (MQ=255)
      aaCATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAAc                                                                                    >  1:10067119/1‑94 (MQ=255)
       aCATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAAcc                                                                                   >  2:2361727/1‑94 (MQ=255)
        cATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCa                                                                                  <  2:543358/94‑1 (MQ=255)
        cATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCa                                                                                  >  2:3409609/1‑94 (MQ=255)
            aaGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATc                                                                              <  1:4807949/94‑1 (MQ=255)
            aaGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATc                                                                              >  2:4880582/1‑94 (MQ=255)
            aaGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATc                                                                              >  2:8679066/1‑94 (MQ=255)
              gggTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCa                                                                            >  1:2969547/1‑94 (MQ=255)
                gTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAcc                                                                          >  2:3130858/1‑94 (MQ=255)
                gTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAcc                                                                          <  1:3388963/94‑1 (MQ=255)
                 tAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCCACTAAACCAAATCCAccg                                                                         >  1:426964/1‑94 (MQ=255)
                 tAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccg                                                                         <  1:5494805/94‑1 (MQ=255)
                 tAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCACCACAGCCGGCAACTAAACCAAATCCAccc                                                                         >  1:7634060/1‑93 (MQ=255)
                  aaaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAATCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccgc                                                                        >  1:9697735/1‑94 (MQ=255)
                  aaaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccgc                                                                        <  1:4073203/94‑1 (MQ=255)
                   aaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccgcc                                                                       >  2:10585401/1‑94 (MQ=255)
                   aaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccgcc                                                                       >  1:2484670/1‑94 (MQ=255)
                   aaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccgcc                                                                       >  2:9266491/1‑94 (MQ=255)
                    aCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccgccg                                                                      <  1:1895618/94‑1 (MQ=255)
                     cGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACcgccgc                                                                     <  1:1228152/94‑1 (MQ=255)
                      gCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCcgcg                                                                    <  1:8115007/94‑1 (MQ=255)
                      gCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCcgcg                                                                    >  2:3595469/1‑94 (MQ=255)
                      gCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCcgcg                                                                    <  1:4645412/94‑1 (MQ=255)
                        tGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAc                                                                  <  2:1003286/94‑1 (MQ=255)
                        tGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAc                                                                  >  2:10105916/1‑94 (MQ=255)
                        tGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAc                                                                  <  1:3482723/94‑1 (MQ=255)
                        tGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAc                                                                  >  2:6996801/1‑94 (MQ=255)
                        tGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAc                                                                  <  1:8881387/94‑1 (MQ=255)
                         gATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAca                                                                 <  1:6238646/94‑2 (MQ=255)
                         gATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACg                                                                 <  1:5719945/94‑1 (MQ=255)
                          aTGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACgg                                                                >  1:7870482/1‑94 (MQ=255)
                            gTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACcgcg                                                              >  2:2542138/1‑94 (MQ=255)
                              cATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGaa                                                            >  2:8067788/1‑94 (MQ=255)
                                tttCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGt                                                          <  1:642242/94‑1 (MQ=255)
                                 ttCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGtt                                                         <  2:1097325/94‑1 (MQ=255)
                                 ttCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGtt                                                         <  1:7041093/94‑1 (MQ=255)
                                 ttCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGCCAGCACAGGCGCTAACTAAACCAACTCCACCGCCGCGACGGCGAAGtt                                                         >  2:9492614/1‑94 (MQ=255)
                                   ccATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCa                                                       >  2:7624237/1‑94 (MQ=255)
                                    cATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCAt                                                      >  2:7421264/1‑94 (MQ=255)
                                    cATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCAt                                                      <  2:4575679/94‑1 (MQ=255)
                                     attattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATg                                                     <  1:5061901/94‑1 (MQ=255)
                                     attattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATg                                                     >  1:4906749/1‑94 (MQ=255)
                                      ttattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGc                                                    <  1:3288871/94‑1 (MQ=255)
                                       tattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCa                                                   <  1:1653097/94‑1 (MQ=255)
                                       tattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCa                                                   <  1:6529221/94‑1 (MQ=255)
                                       tattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCa                                                   >  1:1134679/1‑94 (MQ=255)
                                          tCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATg                                                >  2:1514099/1‑94 (MQ=255)
                                            cGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTc                                              >  2:3272262/1‑94 (MQ=255)
                                             gCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCt                                             >  1:3330856/1‑94 (MQ=255)
                                              ccTCCAGGACCATTGCCGCGCCAGGCCCGCCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTg                                            <  1:4448871/94‑1 (MQ=255)
                                                tCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATTTCTggg                                          >  1:7998484/1‑94 (MQ=255)
                                                tCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTggg                                          >  2:4493367/1‑94 (MQ=255)
                                                tCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGACGCGACGGCGTAGTTCATGCAATGTCTggg                                          >  2:7114070/1‑94 (MQ=255)
                                                 ccAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGt                                         <  2:1646819/94‑1 (MQ=255)
                                                   aGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTaa                                       >  2:1393110/1‑94 (MQ=255)
                                                       ccATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAatcat                                   <  1:3045359/94‑1 (MQ=255)
                                                        cATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAatcatc                                  >  1:2595385/1‑94 (MQ=255)
                                                        cATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGAAataatc                                  >  2:2044390/1‑94 (MQ=255)
                                                         cttGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATcc                                 <  1:3797254/93‑1 (MQ=255)
                                                         aTTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATcc                                 >  2:5020117/1‑94 (MQ=255)
                                                         aTTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATcc                                 >  2:372845/1‑94 (MQ=255)
                                                          ttGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCcg                                <  2:5888224/94‑1 (MQ=255)
                                                              cgcgCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCcgcgcg                            <  1:6926180/94‑1 (MQ=255)
                                                              cgcgCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCcgcgcg                            <  2:6677777/94‑1 (MQ=255)
                                                              cgcgCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCcgcgcg                            <  1:8333440/94‑1 (MQ=255)
                                                               gcgcCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCgctcgc                           <  2:9020825/94‑1 (MQ=255)
                                                                cgcCAAGCCCACTGGCAGCACAGGCGGCAACCAAACCAAATCCTCCGCCGCGACGGCGAAGTTCATGCAATGCCTGGGTAATCATCCGCGCGcc                          <  2:614709/94‑1 (MQ=255)
                                                                cgcCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGcc                          >  2:8930107/1‑94 (MQ=255)
                                                                cgcCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGcc                          >  1:2793115/1‑94 (MQ=255)
                                                                 gcCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCg                         <  2:6247513/94‑1 (MQ=255)
                                                                 gcCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCg                         >  2:10347264/1‑94 (MQ=255)
                                                                  ccAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCgg                        >  2:8542148/1‑94 (MQ=255)
                                                                  ccAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCgg                        >  1:4169356/1‑94 (MQ=255)
                                                                   cAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGt                       >  1:9477493/1‑94 (MQ=255)
                                                                    aaGCCCCCCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTc                      <  1:2339569/94‑1 (MQ=255)
                                                                    aaGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTc                      <  2:9760647/94‑1 (MQ=255)
                                                                     aGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcg                     <  1:1401056/94‑1 (MQ=255)
                                                                     aGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcg                     >  1:8219907/1‑94 (MQ=255)
                                                                      gCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcgc                    <  2:2655178/94‑1 (MQ=255)
                                                                       cccACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcgcc                   <  1:2112856/94‑1 (MQ=255)
                                                                        ccACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcgccg                  >  2:2742226/1‑94 (MQ=255)
                                                                          aCCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCcgcg                >  1:9365059/1‑94 (MQ=255)
                                                                          aCCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCcgcg                >  1:5792540/1‑94 (MQ=255)
                                                                           ccGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGa               >  1:7272238/1‑94 (MQ=255)
                                                                            cGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCTGTCGCCGCGaa              >  2:5734405/1‑94 (MQ=255)
                                                                            cGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGaa              <  1:2241669/94‑1 (MQ=255)
                                                                             ggCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAg             <  2:6832855/94‑1 (MQ=255)
                                                                             ggCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAg             <  1:9164633/94‑1 (MQ=255)
                                                                              gcagcaCAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAgg            <  2:401721/94‑1 (MQ=255)
                                                                              gcagcaCAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAgg            <  1:8666079/94‑1 (MQ=255)
                                                                              gcagcaCAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAgg            <  1:8286912/94‑1 (MQ=255)
                                                                               cagcaCAGGCGGCAACTAAACCTAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAggg           >  1:10430902/1‑94 (MQ=255)
                                                                                agcaCAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGa          >  2:9734993/1‑94 (MQ=255)
                                                                                 gcaCAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGAt         <  2:4407799/94‑1 (MQ=255)
                                                                                  cacaGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATg        <  2:6482881/94‑1 (MQ=255)
                                                                                    caGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGcc      >  2:4214998/1‑94 (MQ=255)
                                                                                     aGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGccc     <  1:5139016/94‑1 (MQ=255)
                                                                                       gCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTCATCATCTGCGCGCCGGTCGCCGCGACGGGCTGCCCGt   >  2:9440358/1‑94 (MQ=255)
                                                                                           |                                                                                          
CAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTA  >  NC_000913/2457141‑2457322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGC  >  NC_000913/2457139‑2457325
                                                                                             |                                                                                             
TCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGC                                                                                               >  SRR4302545.10225301/1‑94 (MQ=60)
TCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGC                                                                                               <  SRR4302545.7550152/94‑1 (MQ=60)
 CCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCA                                                                                              <  SRR4302545.8043948/94‑1 (MQ=60)
  CAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAA                                                                                             >  SRR4302545.10794744/1‑94 (MQ=60)
  CAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAA                                                                                             <  SRR4302545.34321/94‑1 (MQ=60)
   AGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAAC                                                                                            >  SRR4302545.8711359/1‑94 (MQ=60)
    AACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGACCCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACT                                                                                           <  SRR4302545.9324829/94‑1 (MQ=60)
      CGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAA                                                                                         <  SRR4302545.7675835/94‑1 (MQ=60)
        AACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAAC                                                                                       >  SRR4302545.10365018/1‑94 (MQ=60)
        AACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAAC                                                                                       >  SRR4302545.1938120/1‑94 (MQ=60)
            TTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAA                                                                                   >  SRR4302545.8503206/1‑94 (MQ=60)
            TTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAA                                                                                   >  SRR4302545.4817404/1‑94 (MQ=60)
             TAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAAT                                                                                  >  SRR4302545.12588/1‑94 (MQ=60)
             TAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAAT                                                                                  <  SRR4302545.3062401/94‑1 (MQ=60)
             TAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAAT                                                                                  <  SRR4302545.3062399/94‑1 (MQ=60)
              AAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATC                                                                                 <  SRR4302545.3377914/94‑1 (MQ=60)
              AAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATC                                                                                 <  SRR4302545.4930806/94‑1 (MQ=60)
               AGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCTAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCC                                                                                <  SRR4302545.1454095/94‑1 (MQ=60)
               AGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCC                                                                                <  SRR4302545.2798864/94‑1 (MQ=60)
               AGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCC                                                                                <  SRR4302545.2872049/94‑1 (MQ=60)
               AGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCC                                                                                <  SRR4302545.1454096/94‑1 (MQ=60)
               AGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCC                                                                                <  SRR4302545.7407278/94‑1 (MQ=60)
                GGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCA                                                                               >  SRR4302545.3041888/1‑94 (MQ=60)
                 GGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAC                                                                              <  SRR4302545.9521416/94‑1 (MQ=60)
                  GTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACC                                                                             <  SRR4302545.1988555/94‑1 (MQ=60)
                  GTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACC                                                                             <  SRR4302545.3471555/94‑1 (MQ=60)
                  GTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACC                                                                             <  SRR4302545.5925871/94‑1 (MQ=60)
                  GTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATACACC                                                                             >  SRR4302545.4296025/1‑94 (MQ=60)
                   TAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCCACTAAACCAAATCCACCG                                                                            >  SRR4302545.436460/1‑94 (MQ=60)
                   TAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCG                                                                            <  SRR4302545.5639413/94‑1 (MQ=60)
                   TAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCACCACAGCCGGCAACTAAACCAAATCCACCC                                                                            >  SRR4302545.7850092/1‑94 (MQ=60)
                    AAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAATCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGC                                                                           >  SRR4302545.9986008/1‑94 (MQ=60)
                    AAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGC                                                                           <  SRR4302545.4175187/94‑1 (MQ=60)
                    AAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGC                                                                           >  SRR4302545.328526/1‑94 (MQ=60)
                     AACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCGAATCCACCGCC                                                                          <  SRR4302545.8994009/94‑1 (MQ=60)
                     AACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCC                                                                          >  SRR4302545.2544748/1‑94 (MQ=60)
                      ACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCG                                                                         <  SRR4302545.1942346/94‑1 (MQ=60)
                       CGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGC                                                                        <  SRR4302545.1255951/94‑1 (MQ=60)
                       CGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGC                                                                        >  SRR4302545.8353559/1‑94 (MQ=60)
                        GCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCG                                                                       <  SRR4302545.4764087/94‑1 (MQ=60)
                        GCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCG                                                                       <  SRR4302545.8349141/94‑1 (MQ=60)
                          TGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAC                                                                     <  SRR4302545.9139468/94‑1 (MQ=60)
                          TGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAC                                                                     <  SRR4302545.3568098/94‑1 (MQ=60)
                          TGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAC                                                                     <  SRR4302545.8293893/94‑1 (MQ=60)
                           GATGTCATTTCCATTATTCCGCCTCCAGACCCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACG                                                                    <  SRR4302545.3612092/94‑1 (MQ=60)
                           GATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACG                                                                    <  SRR4302545.6198627/94‑1 (MQ=60)
                           GATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACG                                                                    <  SRR4302545.5873359/94‑1 (MQ=60)
                           GATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACG                                                                    <  SRR4302545.7136818/94‑1 (MQ=60)
                           GATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACA                                                                    <  SRR4302545.6408684/94‑1 (MQ=60)
                            ATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGG                                                                   >  SRR4302545.1259178/1‑94 (MQ=60)
                            ATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGG                                                                   >  SRR4302545.8093781/1‑94 (MQ=60)
                            ATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGG                                                                   >  SRR4302545.3552983/1‑94 (MQ=60)
                              GTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCG                                                                 <  SRR4302545.7733243/94‑1 (MQ=60)
                              GTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCG                                                                 <  SRR4302545.2383174/94‑1 (MQ=60)
                               TCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGA                                                                <  SRR4302545.5781351/94‑1 (MQ=60)
                               GCATTTCCATTATAACGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGTCGGCAACTAAACCAAATCCACCGCCGCGACGGCGA                                                                <  SRR4302545.2138741/94‑1 (MQ=60)
                                CATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAA                                                               >  SRR4302545.2218670/1‑94 (MQ=60)
                                CATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAA                                                               >  SRR4302545.4445003/1‑94 (MQ=60)
                                  TTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGT                                                             >  SRR4302545.997751/1‑94 (MQ=60)
                                  TTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGT                                                             <  SRR4302545.656590/94‑1 (MQ=60)
                                  TTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATACACCCCCGCGACGGCGCAGT                                                             >  SRR4302545.7850123/1‑94 (MQ=60)
                                   TTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTT                                                            <  SRR4302545.7235589/94‑1 (MQ=60)
                                   TTCCATTATACCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTT                                                            <  SRR4302545.7235590/94‑1 (MQ=60)
                                     CCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCA                                                          >  SRR4302545.2636107/1‑94 (MQ=60)
                                      CATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCAT                                                         <  SRR4302545.8415484/94‑1 (MQ=60)
                                      CATTATCCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCAT                                                         <  SRR4302545.5533050/94‑1 (MQ=60)
                                      CATTATCCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAAGCCGCCGCCGCGACGGCGAAGTTCAT                                                         <  SRR4302545.8415483/94‑1 (MQ=60)
                                       ATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATG                                                        <  SRR4302545.7153330/94‑1 (MQ=60)
                                       ATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATG                                                        <  SRR4302545.5190745/94‑1 (MQ=60)
                                       ATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATG                                                        <  SRR4302545.4664826/94‑1 (MQ=60)
                                       ATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATG                                                        >  SRR4302545.1366098/1‑94 (MQ=60)
                                       ATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATG                                                        >  SRR4302545.5031286/1‑94 (MQ=60)
                                       ATTATCCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATG                                                        <  SRR4302545.5190746/94‑1 (MQ=60)
                                        TTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGC                                                       <  SRR4302545.3369399/94‑1 (MQ=60)
                                        TTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGC                                                       <  SRR4302545.3015139/94‑1 (MQ=60)
                                         TATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCA                                                      <  SRR4302545.1692231/94‑1 (MQ=60)
                                         TATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCA                                                      <  SRR4302545.6707822/94‑1 (MQ=60)
                                         TATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCA                                                      >  SRR4302545.1159512/1‑94 (MQ=60)
                                         TATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCA                                                      <  SRR4302545.6707823/94‑1 (MQ=60)
                                         TATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCA                                                      >  SRR4302545.7012473/1‑94 (MQ=60)
                                           TTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAAT                                                    <  SRR4302545.4612198/94‑1 (MQ=60)
                                           TTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAAT                                                    <  SRR4302545.2414807/94‑1 (MQ=60)
                                            TCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATG                                                   <  SRR4302545.7591678/94‑1 (MQ=60)
                                             CCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGT                                                  >  SRR4302545.4242292/1‑94 (MQ=60)
                                              CGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGCAGTTCATGCAATGTC                                                 >  SRR4302545.4327044/1‑94 (MQ=60)
                                               GCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCT                                                >  SRR4302545.3412270/1‑94 (MQ=60)
                                               GCCTCCAGAACCATGGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCT                                                <  SRR4302545.31362/94‑1 (MQ=60)
                                                CCTCCAGGACCATTGCCGCGCCAGGCCCGCCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTG                                               <  SRR4302545.4562246/94‑1 (MQ=60)
                                                CCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTG                                               >  SRR4302545.10443930/1‑94 (MQ=60)
                                                 TTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCAGCGCCGGCGACGTTCATGCAATGGCTGG                                              >  SRR4302545.5205643/1‑94 (MQ=60)
                                                 CTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCTAAGTTCATGCAATGTCTGG                                              <  SRR4302545.1725661/94‑1 (MQ=60)
                                                 CTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGG                                              >  SRR4302545.399160/1‑94 (MQ=60)
                                                  TCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATTTCTGGG                                             >  SRR4302545.8226088/1‑94 (MQ=60)
                                                  TCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGG                                             >  SRR4302545.8226087/1‑94 (MQ=60)
                                                   CCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACAAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGT                                            <  SRR4302545.5710097/94‑1 (MQ=60)
                                                     AGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAA                                          <  SRR4302545.1233467/94‑1 (MQ=60)
                                                     AGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAA                                          <  SRR4302545.9854229/94‑1 (MQ=60)
                                                        ACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCA                                       >  SRR4302545.5123605/1‑94 (MQ=60)
                                                        ACCATTGCAGCGCCAAGCCCACCGGCAGCACAGGCAGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCTATGTCTGGGTAATCA                                       <  SRR4302545.7629168/94‑1 (MQ=60)
                                                         CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCAT                                      <  SRR4302545.8753525/94‑1 (MQ=60)
                                                         CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCAT                                      <  SRR4302545.3510318/94‑1 (MQ=60)
                                                         CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCAT                                      <  SRR4302545.3119996/94‑1 (MQ=60)
                                                          CATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATC                                     >  SRR4302545.2657857/1‑94 (MQ=60)
                                                          CATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATC                                     <  SRR4302545.4776354/94‑1 (MQ=60)
                                                          CATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATC                                     <  SRR4302545.3902049/94‑1 (MQ=60)
                                                           CTTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCC                                    <  SRR4302545.3891394/94‑1 (MQ=60)
                                                           ATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCC                                    >  SRR4302545.9480697/1‑94 (MQ=60)
                                                            TTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCG                                   <  SRR4302545.9725352/94‑1 (MQ=60)
                                                            TTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCG                                   <  SRR4302545.2399855/94‑1 (MQ=60)
                                                              GCCGCTCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCCCCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCG                                 <  SRR4302545.5918929/94‑1 (MQ=60)
                                                              GCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCG                                 >  SRR4302545.3578754/1‑94 (MQ=60)
                                                               CCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGC                                >  SRR4302545.281586/1‑94 (MQ=60)
                                                               CCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGC                                <  SRR4302545.8534425/94‑1 (MQ=60)
                                                               CCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGC                                <  SRR4302545.8534754/94‑1 (MQ=60)
                                                                CGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCG                               <  SRR4302545.8574915/94‑1 (MQ=60)
                                                                CGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCG                               <  SRR4302545.2646941/94‑1 (MQ=60)
                                                                CGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCG                               <  SRR4302545.7117043/94‑1 (MQ=60)
                                                                  CGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCC                             >  SRR4302545.2860624/1‑94 (MQ=60)
                                                                   GCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCG                            <  SRR4302545.497889/94‑1 (MQ=60)
                                                                   GCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCG                            >  SRR4302545.445153/1‑94 (MQ=60)
                                                                    CCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGG                           >  SRR4302545.4273401/1‑94 (MQ=60)
                                                                     CAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCTTACTTCATGCAACGTCTGGGAAATCATCTCCGCGCCGGT                          >  SRR4302545.9756325/1‑94 (MQ=60)
                                                                     CAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGT                          >  SRR4302545.9756324/1‑94 (MQ=60)
                                                                      AAGCCCCCCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTC                         <  SRR4302545.2396526/94‑1 (MQ=60)
                                                                      AAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTC                         >  SRR4302545.8515939/1‑94 (MQ=60)
                                                                       AGCCCGCCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCG                        <  SRR4302545.5083366/94‑1 (MQ=60)
                                                                       AGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCG                        >  SRR4302545.7760820/1‑94 (MQ=60)
                                                                       AGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCG                        <  SRR4302545.1432618/94‑1 (MQ=60)
                                                                       AGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCG                        >  SRR4302545.530472/1‑94 (MQ=60)
                                                                       AGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCG                        >  SRR4302545.3402725/1‑94 (MQ=60)
                                                                       AGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCG                        >  SRR4302545.8457876/1‑94 (MQ=60)
                                                                         CCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCC                      <  SRR4302545.2164748/94‑1 (MQ=60)
                                                                         CCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCC                      <  SRR4302545.2226145/94‑1 (MQ=60)
                                                                            ACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCG                   >  SRR4302545.9639797/1‑94 (MQ=60)
                                                                            ACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCG                   >  SRR4302545.5948309/1‑94 (MQ=60)
                                                                             CCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGA                  >  SRR4302545.9105569/1‑94 (MQ=60)
                                                                             CCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGA                  >  SRR4302545.7476140/1‑94 (MQ=60)
                                                                              CGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAA                 <  SRR4302545.2296290/94‑1 (MQ=60)
                                                                              CGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAA                 >  SRR4302545.7089704/1‑94 (MQ=60)
                                                                              CGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAA                 <  SRR4302545.8072836/94‑1 (MQ=60)
                                                                               GGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAG                >  SRR4302545.6471013/1‑94 (MQ=60)
                                                                               GGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAG                <  SRR4302545.9433587/94‑1 (MQ=60)
                                                                                GCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGG               <  SRR4302545.8526963/94‑1 (MQ=60)
                                                                                GCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGG               <  SRR4302545.8917445/94‑1 (MQ=60)
                                                                                 CAGCACAGGCGGCAACTTTACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCTGGCCGCCGCGAAGGG              >  SRR4302545.9688606/1‑94 (MQ=60)
                                                                                 CAGCACAGGCGGCAACTAAACCTAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGG              >  SRR4302545.10738327/1‑94 (MQ=60)
                                                                                  AGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGA             >  SRR4302545.6468562/1‑94 (MQ=60)
                                                                                   GCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGAT            <  SRR4302545.1077126/94‑1 (MQ=60)
                                                                                   GCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGAT            <  SRR4302545.9185128/94‑1 (MQ=60)
                                                                                    CACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATG           <  SRR4302545.10124247/94‑1 (MQ=60)
                                                                                    CACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATG           >  SRR4302545.21215/1‑94 (MQ=60)
                                                                                    CACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATG           <  SRR4302545.8125039/94‑1 (MQ=60)
                                                                                      CAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCC         <  SRR4302545.4210736/94‑1 (MQ=60)
                                                                                       AGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCC        <  SRR4302545.5270988/94‑1 (MQ=60)
                                                                                       AGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCC        >  SRR4302545.6350226/1‑94 (MQ=60)
                                                                                             CAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGC  <  SRR4302545.8371260/94‑1 (MQ=60)
                                                                                             CAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGC  >  SRR4302545.603222/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGC  >  NC_000913/2457139‑2457325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑