Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,457,232 T→C T420A (ACC→GCC)  b2342 ← putative acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,457,2320TC100.0% 713.6 / NA 205T420A (ACC→GCC) b2342putative acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (124/81);  total (124/81)

Only 100 of 213 total aligned reads displayed.

GACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGT  >  NC_000913/2457143‑2457321
                                                                                         |                                                                                         
gACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAAc                                                                                        >  2:3483899/1‑93 (MQ=255)
  cGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTa                                                                                      <  1:6015788/93‑1 (MQ=255)
   gAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTaa                                                                                     <  1:3002651/93‑1 (MQ=255)
    aaCATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTaaa                                                                                    <  2:4057045/93‑1 (MQ=255)
      cATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAAcc                                                                                  <  2:3196993/93‑1 (MQ=255)
        ttAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCaa                                                                                >  2:5027360/1‑93 (MQ=255)
          aaGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAAt                                                                              >  1:2084674/1‑93 (MQ=255)
          aaGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAAt                                                                              <  1:523469/93‑1 (MQ=255)
           aGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATc                                                                             <  2:286398/93‑1 (MQ=255)
            gggTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATcc                                                                            <  1:1005713/93‑1 (MQ=255)
            gggTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATcc                                                                            >  2:4534680/1‑93 (MQ=255)
            gggTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATcc                                                                            >  1:354309/1‑93 (MQ=255)
             ggTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCa                                                                           <  1:3926118/93‑1 (MQ=255)
             ggTAAACGCTGATGTCATTTCCATTATTCCGCCCCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAAACAAATCCa                                                                           >  1:1492383/1‑93 (MQ=255)
               tAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAcc                                                                         <  2:2114845/93‑1 (MQ=255)
                aaaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccg                                                                        >  2:4239544/1‑93 (MQ=255)
                aaaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccg                                                                        <  2:1242440/93‑1 (MQ=255)
                aaaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATACAacg                                                                        >  2:1044683/1‑93 (MQ=255)
                 aaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccgc                                                                       <  1:6406480/93‑1 (MQ=255)
                 aaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccgc                                                                       <  2:1166010/93‑1 (MQ=255)
                 aaCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccgc                                                                       >  2:2976995/1‑93 (MQ=255)
                  aCGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAccgcc                                                                      <  2:5284441/93‑1 (MQ=255)
                    gCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACcgccgc                                                                    >  2:1435343/1‑93 (MQ=255)
                    gCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACcgccgc                                                                    >  1:1245114/1‑93 (MQ=255)
                    gCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACcgccgc                                                                    >  1:499364/1‑93 (MQ=255)
                     cTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCcgcg                                                                   <  2:2603731/93‑1 (MQ=255)
                     cTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCcgcg                                                                   >  1:6294294/1‑93 (MQ=255)
                      tGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGa                                                                  >  2:223874/1‑93 (MQ=255)
                      tGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGa                                                                  <  2:633401/93‑1 (MQ=255)
                      tGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGa                                                                  >  2:1954393/1‑93 (MQ=255)
                      tGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGa                                                                  >  1:2650450/1‑93 (MQ=255)
                       gATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAc                                                                 >  2:4403285/1‑93 (MQ=255)
                       gATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAc                                                                 >  2:6243132/1‑93 (MQ=255)
                       gATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAc                                                                 <  1:4156641/93‑1 (MQ=255)
                        aTGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACg                                                                <  1:1613961/93‑1 (MQ=255)
                        aTGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACg                                                                >  2:4230689/1‑93 (MQ=255)
                          gTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGc                                                              >  2:4470445/1‑93 (MQ=255)
                           tCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCg                                                             >  2:3635954/1‑93 (MQ=255)
                              tttCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAg                                                          >  1:6254130/1‑93 (MQ=255)
                                tCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGtt                                                        <  1:5369311/93‑1 (MQ=255)
                                 ccATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTc                                                       >  1:5180976/1‑93 (MQ=255)
                                  cATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCa                                                      >  2:4674899/1‑93 (MQ=255)
                                  cATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCa                                                      >  2:4903368/1‑93 (MQ=255)
                                   attattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCAt                                                     >  2:3257419/1‑93 (MQ=255)
                                   actattCCGCCTCCAGAACCATTGCCACGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCAt                                                     <  2:715621/91‑1 (MQ=255)
                                    ttattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGTCAACTAAACCAACCCCCCCGCCGCTACGGCGAAGTTCATg                                                    <  2:1496376/93‑1 (MQ=255)
                                    ttattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCTGCGACGGCGAAGTTCATg                                                    >  1:3174788/1‑93 (MQ=255)
                                    ttattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATg                                                    >  2:6055693/1‑93 (MQ=255)
                                    ttattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATg                                                    <  1:742534/93‑1 (MQ=255)
                                     tattCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGc                                                   >  1:303987/1‑93 (MQ=255)
                                      attCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACAGCCGCGACGGCGAAGTTCATGCa                                                  >  1:558941/1‑93 (MQ=255)
                                       ttCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATACACCGCCGCGACGGCGAAGTTCATGCaa                                                 >  1:1915858/1‑93 (MQ=255)
                                           gCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTc                                             <  2:2285302/93‑1 (MQ=255)
                                            ccTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCt                                            >  1:2298460/1‑93 (MQ=255)
                                              tCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTgg                                          >  2:5364705/1‑93 (MQ=255)
                                              tCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTgg                                          >  2:2499426/1‑93 (MQ=255)
                                              tCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTgg                                          >  2:1854980/1‑93 (MQ=255)
                                               ccAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTggg                                         >  1:270335/1‑93 (MQ=255)
                                               ccAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTggg                                         >  1:242375/1‑93 (MQ=255)
                                               ccAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTggg                                         <  1:4754918/93‑1 (MQ=255)
                                                 aGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTa                                       >  1:3410794/1‑93 (MQ=255)
                                                  gAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTaa                                      >  1:4457317/1‑93 (MQ=255)
                                                   aaCCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAat                                     >  2:6101798/1‑93 (MQ=255)
                                                   aaCCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAat                                     <  1:5066100/93‑1 (MQ=255)
                                                   aaCCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAat                                     >  2:5457283/1‑93 (MQ=255)
                                                   aaCCATGGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAat                                     <  1:5272923/93‑1 (MQ=255)
                                                    aCCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAatc                                    >  1:4608560/1‑93 (MQ=255)
                                                    aCCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAata                                    >  2:3880035/1‑92 (MQ=255)
                                                    aCCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAAACCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAatc                                    <  1:6466875/93‑1 (MQ=255)
                                                     ccATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAatca                                   <  1:583136/93‑1 (MQ=255)
                                                      cATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTaataat                                  >  2:1267230/1‑93 (MQ=255)
                                                       aTTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAatcatc                                 >  1:894035/1‑93 (MQ=255)
                                                       aTTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAatcatc                                 >  1:4244713/1‑93 (MQ=255)
                                                       aTTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAatcatc                                 <  2:3687840/93‑1 (MQ=255)
                                                        ttGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATcc                                <  2:3339089/93‑1 (MQ=255)
                                                           ccGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCcgcg                             >  1:3789801/1‑93 (MQ=255)
                                                            cgcgCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCcgcgc                            <  1:5606871/93‑1 (MQ=255)
                                                              cgcCAAACCCGCCGCCAGCACAGTCGGCAACTAAAGCAAAGCCACCCCCGCGACGGCGGAGTCCATGCAATGTCTGCGTAATCATCCgcgcgc                          <  2:1607611/93‑1 (MQ=255)
                                                                     cccACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcgc                   <  2:5774561/93‑1 (MQ=255)
                                                                      ccACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcgcc                  >  2:1786498/1‑93 (MQ=255)
                                                                      ccACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcgcc                  >  2:2711766/1‑93 (MQ=255)
                                                                       cACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcgccg                 >  2:840760/1‑93 (MQ=255)
                                                                       cACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcgccg                 >  2:3664986/1‑93 (MQ=255)
                                                                        aCCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcgccgc                <  1:3683666/93‑1 (MQ=255)
                                                                        aCCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTcgccgc                >  1:5912610/1‑93 (MQ=255)
                                                                         ccGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCcgcg               >  2:2439502/1‑93 (MQ=255)
                                                                           ggCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGaa             <  1:2519714/93‑1 (MQ=255)
                                                                           ggCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGaa             <  1:2750624/93‑1 (MQ=255)
                                                                           ggCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGaa             <  1:282106/93‑1 (MQ=255)
                                                                           ggCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGaa             <  1:6235718/93‑1 (MQ=255)
                                                                             cagcaCAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAgg           <  1:4589462/93‑1 (MQ=255)
                                                                              agcaCAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAggg          >  2:4504293/1‑93 (MQ=255)
                                                                               gcaCAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGa         <  1:4419744/93‑1 (MQ=255)
                                                                               gcaCAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGa         <  1:2912592/93‑1 (MQ=255)
                                                                               gcaCAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGa         >  1:960235/1‑93 (MQ=255)
                                                                               gcaCAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGa         >  1:1231261/1‑93 (MQ=255)
                                                                                cacaGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGAt        <  2:5008605/93‑1 (MQ=255)
                                                                                cacaGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGAt        >  2:1832308/1‑93 (MQ=255)
                                                                                 acaGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATg       >  2:4798947/1‑93 (MQ=255)
                                                                                 acaGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCAGGTCGCCGCGAAGGGATg       >  1:1843050/1‑93 (MQ=255)
                                                                                         |                                                                                         
GACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGT  >  NC_000913/2457143‑2457321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAA  >  NC_000913/2457139‑2457323
                                                                                             |                                                                                           
TCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGC                                                                                             >  SRR4302537.4130585/1‑94 (MQ=60)
 CCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCA                                                                                            >  SRR4302537.4992576/1‑94 (MQ=60)
    GACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACT                                                                                         <  SRR4302537.5851538/94‑1 (MQ=60)
      CGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCATCGGCAGCACAGGCGGCAACTAA                                                                                       >  SRR4302537.5901655/1‑94 (MQ=60)
      CGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAA                                                                                       >  SRR4302537.2835816/1‑94 (MQ=60)
      CGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAA                                                                                       <  SRR4302537.6057121/94‑1 (MQ=60)
       GAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAA                                                                                      <  SRR4302537.3023569/94‑1 (MQ=60)
        AACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAAC                                                                                     >  SRR4302537.4638136/1‑94 (MQ=60)
        AACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAAC                                                                                     <  SRR4302537.5659270/94‑1 (MQ=60)
          CATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCA                                                                                   >  SRR4302537.5424903/1‑94 (MQ=60)
            TTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAA                                                                                 >  SRR4302537.3022123/1‑94 (MQ=60)
            TTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAA                                                                                 >  SRR4302537.238544/1‑94 (MQ=60)
             TAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAAT                                                                                >  SRR4302537.6489371/1‑94 (MQ=60)
             TAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAAT                                                                                >  SRR4302537.2099363/1‑94 (MQ=60)
              AAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATC                                                                               <  SRR4302537.527061/94‑1 (MQ=60)
               AGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCC                                                                              <  SRR4302537.1188540/94‑1 (MQ=60)
               AGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCC                                                                              >  SRR4302537.356775/1‑94 (MQ=60)
               AGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCC                                                                              >  SRR4302537.4183321/1‑94 (MQ=60)
                GGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCA                                                                             <  SRR4302537.5628935/94‑1 (MQ=60)
                GGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCA                                                                             <  SRR4302537.1012654/94‑1 (MQ=60)
                GGGTAAACGCTGATGTCATTTCCATTATTCCGCCCCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAAACAAATCCA                                                                             >  SRR4302537.1502778/1‑94 (MQ=60)
                 GGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCAC                                                                            <  SRR4302537.3953548/94‑1 (MQ=60)
                   TAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCG                                                                          >  SRR4302537.3843756/1‑94 (MQ=60)
                     AACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCC                                                                        <  SRR4302537.4292813/94‑1 (MQ=60)
                     AACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCC                                                                        <  SRR4302537.6450083/94‑1 (MQ=60)
                      ACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCG                                                                       <  SRR4302537.5022675/94‑1 (MQ=60)
                       CGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGC                                                                      >  SRR4302537.502795/1‑94 (MQ=60)
                       CGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGC                                                                      >  SRR4302537.1253790/1‑94 (MQ=60)
                        GCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCG                                                                     >  SRR4302537.6337416/1‑94 (MQ=60)
                        GCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCG                                                                     >  SRR4302537.6322408/1‑94 (MQ=60)
                        GCTGATGTCATTTCCATTATTCCGCCTACAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCG                                                                     >  SRR4302537.6181335/1‑94 (MQ=60)
                         CTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGA                                                                    >  SRR4302537.2668992/1‑94 (MQ=60)
                         CTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGA                                                                    >  SRR4302537.1605535/1‑94 (MQ=60)
                         CTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGA                                                                    >  SRR4302537.3781846/1‑94 (MQ=60)
                          TGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGAC                                                                   >  SRR4302537.3086307/1‑94 (MQ=60)
                           GATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACG                                                                  <  SRR4302537.4185668/94‑1 (MQ=60)
                            ATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGG                                                                 <  SRR4302537.1625238/94‑1 (MQ=60)
                            ATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACCG                                                                 >  SRR4302537.2301269/1‑94 (MQ=60)
                             TGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGC                                                                <  SRR4302537.1246455/94‑1 (MQ=60)
                               TCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGA                                                              >  SRR4302537.4550677/1‑94 (MQ=60)
                                 ATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAG                                                            >  SRR4302537.1983316/1‑94 (MQ=60)
                                 ATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAG                                                            <  SRR4302537.3603900/94‑1 (MQ=60)
                                 ATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAG                                                            >  SRR4302537.6297093/1‑94 (MQ=60)
                                  TTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGT                                                           >  SRR4302537.2874841/1‑94 (MQ=60)
                                   TTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTT                                                          >  SRR4302537.4653860/1‑94 (MQ=60)
                                    TCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTC                                                         >  SRR4302537.5216628/1‑94 (MQ=60)
                                    TCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTC                                                         <  SRR4302537.5406045/94‑1 (MQ=60)
                                     CCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCA                                                        <  SRR4302537.3462951/94‑1 (MQ=60)
                                       ATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCTGCGACGGCGAAGTTCATG                                                      >  SRR4302537.3197103/1‑94 (MQ=60)
                                        TTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGC                                                     >  SRR4302537.306101/1‑94 (MQ=60)
                                        TTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGC                                                     <  SRR4302537.747599/94‑1 (MQ=60)
                                         TATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACAGCCGCGACGGCGAAGTTCATGCA                                                    >  SRR4302537.562789/1‑94 (MQ=60)
                                          ATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATACACCGCCGCGACGGCGAAGTTCATGCAA                                                   >  SRR4302537.1929153/1‑94 (MQ=60)
                                               GCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCT                                              >  SRR4302537.2314683/1‑94 (MQ=60)
                                               GCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCT                                              >  SRR4302537.301332/1‑94 (MQ=60)
                                                  TCCAGACCCATTGCCGCGCCAAGCCCGCCGGCAGCACAGGCGGCATCTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGG                                           <  SRR4302537.875699/94‑1 (MQ=60)
                                                  TCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGG                                           >  SRR4302537.1296196/1‑94 (MQ=60)
                                                  TCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGG                                           >  SRR4302537.272238/1‑94 (MQ=60)
                                                  TCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGG                                           >  SRR4302537.244093/1‑94 (MQ=60)
                                                   CCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGT                                          <  SRR4302537.3275445/94‑1 (MQ=60)
                                                   CCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGT                                          <  SRR4302537.4787761/94‑1 (MQ=60)
                                                    CAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTA                                         >  SRR4302537.3434951/1‑94 (MQ=60)
                                                    CAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTA                                         >  SRR4302537.3032432/1‑94 (MQ=60)
                                                     AGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAA                                        >  SRR4302537.4488101/1‑94 (MQ=60)
                                                      GAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAAT                                       <  SRR4302537.2708384/94‑1 (MQ=60)
                                                       AACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATC                                      <  SRR4302537.5100976/94‑1 (MQ=60)
                                                       AACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATC                                      >  SRR4302537.4640471/1‑94 (MQ=60)
                                                       AACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATC                                      <  SRR4302537.4144126/94‑1 (MQ=60)
                                                       AACCATGGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATC                                      <  SRR4302537.5309209/94‑1 (MQ=60)
                                                        ACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCA                                     >  SRR4302537.668032/1‑94 (MQ=60)
                                                        ACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAAACCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCA                                     <  SRR4302537.6510686/94‑1 (MQ=60)
                                                         CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCAT                                    <  SRR4302537.587123/94‑1 (MQ=60)
                                                         CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACAGCGAAGTTCATGCAATGTCTGGGTAATCAT                                    >  SRR4302537.5082921/1‑94 (MQ=60)
                                                          CATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATC                                   >  SRR4302537.900204/1‑94 (MQ=60)
                                                          CATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATC                                   >  SRR4302537.4274215/1‑94 (MQ=60)
                                                           ATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGACGATCATGCAATGTCTGGGTAATCATCC                                  >  SRR4302537.6382136/1‑94 (MQ=60)
                                                           ATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCC                                  <  SRR4302537.5252957/94‑1 (MQ=60)
                                                           ATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCC                                  >  SRR4302537.6011718/1‑94 (MQ=60)
                                                             TGCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGC                                <  SRR4302537.5364462/94‑1 (MQ=60)
                                                              GCCGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCG                               >  SRR4302537.3816400/1‑94 (MQ=60)
                                                                CGCGCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCG                             <  SRR4302537.5645201/94‑1 (MQ=60)
                                                                   GCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGTCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCG                          <  SRR4302537.445806/94‑1 (MQ=60)
                                                                   GCCAAGCCCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCG                          >  SRR4302537.944677/1‑94 (MQ=60)
                                                                          CCACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCG                   >  SRR4302537.1901986/1‑94 (MQ=60)
                                                                           CACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGC                  >  SRR4302537.5953180/1‑94 (MQ=60)
                                                                            ACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCG                 <  SRR4302537.3709570/94‑1 (MQ=60)
                                                                            ACCGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCG                 <  SRR4302537.1001248/94‑1 (MQ=60)
                                                                              CGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAG               >  SRR4302537.2443272/1‑94 (MQ=60)
                                                                              CGGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAA               <  SRR4302537.486945/94‑1 (MQ=60)
                                                                               GGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAG              <  SRR4302537.2159058/94‑1 (MQ=60)
                                                                               GGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAG              <  SRR4302537.274521/94‑1 (MQ=60)
                                                                               GGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAG              <  SRR4302537.2537410/94‑1 (MQ=60)
                                                                               GGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAG              <  SRR4302537.6278601/94‑1 (MQ=60)
                                                                               GGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAG              <  SRR4302537.2769815/94‑1 (MQ=60)
                                                                               GGCAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAG              <  SRR4302537.284072/94‑1 (MQ=60)
                                                                               GGCAGCACAGCCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAG              <  SRR4302537.730208/94‑1 (MQ=60)
                                                                                 CAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGG            <  SRR4302537.4621201/94‑1 (MQ=60)
                                                                                 CAGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGG            <  SRR4302537.5274026/94‑1 (MQ=60)
                                                                                  AGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGA           >  SRR4302537.966855/1‑94 (MQ=60)
                                                                                  AGCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGA           >  SRR4302537.1239822/1‑94 (MQ=60)
                                                                                   GCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGAT          <  SRR4302537.4450258/94‑1 (MQ=60)
                                                                                   GCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGAT          <  SRR4302537.2933050/94‑1 (MQ=60)
                                                                                   GCACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGAT          <  SRR4302537.1669624/94‑1 (MQ=60)
                                                                                    CACAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCAGGTCGCCGCGAAGGGATG         >  SRR4302537.1855878/1‑94 (MQ=60)
                                                                                      CAGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCC       >  SRR4302537.2547010/1‑94 (MQ=60)
                                                                                       AGGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCC      <  SRR4302537.4209314/94‑1 (MQ=60)
                                                                                        GGCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCG     <  SRR4302537.5143435/94‑1 (MQ=60)
                                                                                         GCGGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGT    <  SRR4302537.627907/94‑1 (MQ=60)
                                                                                           GGCAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAA  <  SRR4302537.3078775/94‑1 (MQ=60)
                                                                                             |                                                                                           
TCCAGACGAACATTAAGGGTAAACGCTGATGTCATTTCCATTATTCCGCCTCCAGAACCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACCAAATCCACCGCCGCGACGGCGAAGTTCATGCAATGTCTGGGTAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAA  >  NC_000913/2457139‑2457323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑