Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,458,644	A→G	intergenic (‑155/+26)	b2342 ← / ← b2343	putative acyltransferase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,458,644	0	A	G	96.3%	614.3 / 8.4	189	intergenic (‑155/+26)	b2342/b2343	putative acyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (2/5); new base G (107/75); total (109/80)
Fisher's exact test for biased strand distribution p-value = 1.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

Only 100 of 200 total aligned reads displayed.

ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATC  >  NC_000913/2458554‑2458732

aCATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCa                                                                                        <  2:2615317/93‑1 (MQ=255)

aCATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCa                                                                                        <  2:660958/93‑1 (MQ=255)

aCATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCa                                                                                        >  1:897591/1‑93 (MQ=255)

aCATTTAGCAAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCa                                                                                        <  1:3665071/93‑1 (MQ=255)

 cATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAg                                                                                       >  1:3165047/1‑93 (MQ=255)

 cATTTAGCCAAGTGGAGAAAAGGGAAAGGGGGAGCTATGACACAGAGAAAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAg                                                                                       <  2:370262/93‑1 (MQ=255)

  aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAgg                                                                                      >  2:5025611/1‑93 (MQ=255)

   tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAggg                                                                                     >  1:2992976/1‑93 (MQ=255)

    ttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCGCCTGCCAGCAACTGGCAgggg                                                                                    >  2:1020209/1‑93 (MQ=255)

    ttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAgggg                                                                                    >  2:3794203/1‑93 (MQ=255)

        ccAGGTGGAGAAAAGGAAAAGTGGGAGCTATGACACAGAGAAAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGcgta                                                                                <  1:5915571/93‑1 (MQ=255)

         cAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGACAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGcgtac                                                                               >  1:3464546/1‑93 (MQ=255)

         cAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGcgtac                                                                               <  1:4174258/93‑1 (MQ=255)

         cAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGcgtac                                                                               >  1:2879711/1‑93 (MQ=255)

         cAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGcgtac                                                                               <  1:5057742/93‑1 (MQ=255)

         cAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGcgtac                                                                               >  2:972641/1‑93 (MQ=255)

          aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGcgtacg                                                                              >  2:5313129/1‑93 (MQ=255)

          aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGcgtacg                                                                              >  1:5516983/1‑93 (MQ=255)

          aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGcgtacg                                                                              >  2:6131063/1‑93 (MQ=255)

           aGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGcgtacgt                                                                             >  2:3844816/1‑93 (MQ=255)

            gTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGcgtacgta                                                                            <  2:715569/93‑1 (MQ=255)

              ggAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTAcc                                                                          <  1:6417461/93‑1 (MQ=255)

              gaagaAAAGGAAAAGTGGGAGTTATGACAAAGAGAAAAAAGAAGAAGAAAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTAcc                                                                          <  2:1369423/91‑1 (MQ=255)

                 gaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCga                                                                       >  1:1679161/1‑93 (MQ=255)

                 gaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCga                                                                       <  2:3201934/93‑1 (MQ=255)

                  aaaaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCgcg                                                                      >  2:5886119/1‑91 (MQ=255)

                  aaaaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCgag                                                                      >  1:4836192/1‑93 (MQ=255)

                   aaaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCgaga                                                                     >  1:1614427/1‑93 (MQ=255)

                    aaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGagag                                                                    >  1:5448191/1‑93 (MQ=255)

                     aGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAgg                                                                   >  1:3856203/1‑93 (MQ=255)

                      gggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGa                                                                  >  2:5388191/1‑93 (MQ=255)

                      gggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGa                                                                  >  2:6180217/1‑93 (MQ=255)

                      gggAAAGTGGGAGCTAGGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGa                                                                  <  1:5722345/93‑1 (MQ=255)

                             tGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACg                                                           <  2:3891535/93‑1 (MQ=255)

                              gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGc                                                          <  2:5191353/93‑1 (MQ=255)

                               ggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                         >  2:1856418/1‑93 (MQ=255)

                               ggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                         >  1:3255322/1‑93 (MQ=255)

                                gAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAg                                                        >  2:2115219/1‑93 (MQ=255)

                                 aGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGa                                                       <  2:6283800/93‑1 (MQ=255)

                                  gCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAc                                                      >  2:4299458/1‑93 (MQ=255)

                                    tATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAccc                                                    >  1:1265832/1‑93 (MQ=255)

                                      tGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAg                                                  <  2:1984546/93‑1 (MQ=255)

                                       gACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGc                                                 >  1:6334964/1‑93 (MQ=255)

                                            agagagAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaa                                            >  1:1449670/1‑93 (MQ=255)

                                             gagagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaa                                           >  1:3885661/1‑93 (MQ=255)

                                               aaaaaaaaaAGAAAAAAAAAAAAACCCCCCCGGCCAGCACCGGGCGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAAt                                         <  2:4438315/90‑1 (MQ=255)

                                                 gaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                                       >  2:1227606/1‑93 (MQ=255)

                                                  aaaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAg                                      <  2:4766771/93‑1 (MQ=255)

                                                    aagaagaagAGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCa                                    >  1:4751828/1‑93 (MQ=255)

                                                     agaaaaagaGAAAAGAAACCGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAt                                   <  2:3647914/93‑1 (MQ=255)

                                                      gaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAtt                                  >  2:5350995/1‑93 (MQ=255)

                                                      gaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAtt                                  >  2:4205710/1‑93 (MQ=255)

                                                      gaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAtt                                  >  2:559949/1‑93 (MQ=255)

                                                      gaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAtt                                  >  1:3924470/1‑93 (MQ=255)

                                                       aagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAttt                                 >  1:2677567/1‑93 (MQ=255)

                                                        agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                >  1:2130171/1‑93 (MQ=255)

                                                        agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                >  2:4660114/1‑93 (MQ=255)

                                                        agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                <  2:4099675/93‑1 (MQ=255)

                                                         gaagaGAAAAAAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCa                               <  2:2860603/93‑1 (MQ=255)

                                                          aagaGAAAAAAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAg                              <  1:3564975/93‑1 (MQ=255)

                                                           agagAAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGc                             <  1:2088573/93‑1 (MQ=255)

                                                            gagaAAAGAAAACGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCt                            >  1:2341291/1‑93 (MQ=255)

                                                             aaaaaaaaaaaCCCCCCGGCCAGCACCGGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCtt                           <  2:1131906/91‑1 (MQ=255)

                                                                aaagaaaaCGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                        >  1:3510573/1‑93 (MQ=255)

                                                                 aagaaacCCCCCCTGCCAGCAACTGGCAGGGGCGTACGTCCCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                       <  2:5251192/93‑1 (MQ=255)

                                                                 aagaaaaCGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCGAGCTGGAAAATCAGCATTTCAGCTTCGCa                       <  2:1559125/93‑1 (MQ=255)

                                                                 aagaaaaCGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                       >  1:2731069/1‑93 (MQ=255)

                                                                   gaaaaCGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                     >  1:4484260/1‑93 (MQ=255)

                                                                    aaaaCGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGc                    >  2:685434/1‑93 (MQ=255)

                                                                      aaCGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaa                  >  1:1193249/1‑93 (MQ=255)

                                                                      aaCGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaa                  >  1:1858939/1‑93 (MQ=255)

                                                                       aCGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGGTTCGCAGGCaaa                 >  1:1427224/1‑93 (MQ=255)

                                                                         gCCCCTGCCAGCAACTGGCAGGGGAGTACGTACCCGAGAGGGATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAg               >  2:3393774/1‑93 (MQ=255)

                                                                          ccccTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt              <  2:5746760/93‑1 (MQ=255)

                                                                          ccccTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt              <  2:2254411/93‑1 (MQ=255)

                                                                          ccccTGCCACCAACGGGCGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt              <  2:5498858/93‑1 (MQ=255)

                                                                           cccTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTg             >  1:5606152/1‑93 (MQ=255)

                                                                           cccTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTg             >  1:4316948/1‑93 (MQ=255)

                                                                            ccTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGa            >  1:4229062/1‑93 (MQ=255)

                                                                            ccTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGa            <  1:6179947/93‑1 (MQ=255)

                                                                            ccTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTAGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGa            >  2:5111520/1‑93 (MQ=255)

                                                                             cTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaa           >  1:748371/1‑93 (MQ=255)

                                                                             cTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaa           >  1:4649959/1‑93 (MQ=255)

                                                                              tGCCAGCAACTGGCGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa          <  2:3415303/93‑1 (MQ=255)

                                                                              tGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa          <  2:3973832/93‑1 (MQ=255)

                                                                               gCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAt         <  2:1655478/93‑1 (MQ=255)

                                                                               gCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTAGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAt         <  1:4325806/93‑1 (MQ=255)

                                                                                ccAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATc        <  2:5283766/93‑1 (MQ=255)

                                                                                ccAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATc        >  1:50906/1‑93 (MQ=255)

                                                                                ccAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATc        <  2:4601753/93‑1 (MQ=255)

                                                                                 cAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCg       >  2:1732204/1‑93 (MQ=255)

                                                                                 cAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCg       >  1:3036710/1‑93 (MQ=255)

                                                                                 cAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCg       >  1:1523814/1‑93 (MQ=255)

                                                                                  agcagcTGGCGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGa      <  1:3342065/93‑1 (MQ=255)

                                                                                  aGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGa      >  1:6430284/1‑93 (MQ=255)

                                                                                   gCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGat     <  1:5181499/93‑1 (MQ=255)

                                                                                     aaCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGatat   <  2:2410882/93‑1 (MQ=255)

                                                                                     aaCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGatat   >  2:2527213/1‑93 (MQ=255)

                                                                                     aaCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGatat   >  2:3492811/1‑93 (MQ=255)

                                                                                      aCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATc  <  2:1783991/93‑1 (MQ=255)

ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATC  >  NC_000913/2458554‑2458732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAG  >  NC_000913/2458553‑2458735

TACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCA                                                                                           >  SRR4302537.903784/1‑94 (MQ=60)

TACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCA                                                                                           <  SRR4302537.4736663/94‑1 (MQ=60)

TACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCA                                                                                           >  SRR4302537.1384957/1‑94 (MQ=60)

 ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAG                                                                                          >  SRR4302537.3187273/1‑94 (MQ=60)

 ACATTTAGCAAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAG                                                                                          <  SRR4302537.3690870/94‑1 (MQ=60)

  CATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGG                                                                                         >  SRR4302537.1507436/1‑94 (MQ=60)

  CATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGG                                                                                         >  SRR4302537.5373156/1‑94 (MQ=60)

  CATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGG                                                                                         >  SRR4302537.3228752/1‑94 (MQ=60)

   ATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGG                                                                                        >  SRR4302537.276543/1‑94 (MQ=60)

   ATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGG                                                                                        >  SRR4302537.3013859/1‑94 (MQ=60)

   ATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCACCTGCCAGCAACTGGCAGGG                                                                                        >  SRR4302537.5615980/1‑94 (MQ=60)

    TTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGG                                                                                       >  SRR4302537.5198906/1‑94 (MQ=60)

         CCAGGTGGAGAAAAGGAAAAGTGGGAGCTATGACACAGAGAAAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTAC                                                                                  <  SRR4302537.5956166/94‑1 (MQ=60)

         CCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGACAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTAC                                                                                  >  SRR4302537.3489045/1‑94 (MQ=60)

         CCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTAC                                                                                  >  SRR4302537.2899919/1‑94 (MQ=60)

          CAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACG                                                                                 <  SRR4302537.5092559/94‑1 (MQ=60)

          CAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACG                                                                                 >  SRR4302537.2372063/1‑94 (MQ=60)

          CAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACG                                                                                 <  SRR4302537.4203404/94‑1 (MQ=60)

          CAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACG                                                                                 >  SRR4302537.5554652/1‑94 (MQ=60)

             GTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTAC                                                                              >  SRR4302537.3418774/1‑94 (MQ=60)

               GGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCC                                                                            <  SRR4302537.6461100/94‑1 (MQ=60)

                 AGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAAGTGGCAGGGGCGTACGTACCCGA                                                                          >  SRR4302537.6315678/1‑94 (MQ=60)

                 AGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGA                                                                          >  SRR4302537.1690911/1‑94 (MQ=60)

                  GAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAG                                                                         >  SRR4302537.4869585/1‑94 (MQ=60)

                  GAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAG                                                                         >  SRR4302537.3007922/1‑94 (MQ=60)

                   AAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGA                                                                        >  SRR4302537.1625708/1‑94 (MQ=60)

                    AAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAG                                                                       >  SRR4302537.5485352/1‑94 (MQ=60)

                    AAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAG                                                                       >  SRR4302537.1444960/1‑94 (MQ=60)

                     AAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGG                                                                      >  SRR4302537.3883194/1‑94 (MQ=60)

                       GGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAA                                                                    <  SRR4302537.6424102/94‑1 (MQ=60)

                       GGGAAAGTGGGAGCTAGGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAA                                                                    <  SRR4302537.5761515/94‑1 (MQ=60)

                         gaaggcgtaagcgATGACAAAGAGAGAAAAAAAGAAGAGAAAAGACAA‑CGCCCCTGCCAGCAACTGAGAGGGGCGTACGTACCCGAGAGGAATT                                                                  <  SRR4302537.314353/81‑1 (MQ=60)

                         gaaagggggatataggaaaaaggaaaaaagGAAGAAGAGAAAAAAAAC‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATT                                                                  <  SRR4302537.5911874/64‑1 (MQ=60)

                            AGGGGGAGCTATGACAAAGAGAAAAAAAAAGAAAAAAAAAAAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAAC                                                               <  SRR4302537.232273/94‑1 (MQ=60)

                               GGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCA                                                            >  SRR4302537.3278279/1‑94 (MQ=60)

                                GGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAG                                                           <  SRR4302537.929184/94‑1 (MQ=60)

                                 GAGCAATGACAAAGAAAAAAAAAAAAAAGAAAAAAGAACA‑CCCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGA                                                          <  SRR4302537.3577714/94‑1 (MQ=50)

                                  AGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAC                                                         >  SRR4302537.4422158/1‑94 (MQ=60)

                                    CTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCC                                                       >  SRR4302537.1274634/1‑94 (MQ=60)

                                     TATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCA                                                      <  SRR4302537.1748344/94‑1 (MQ=60)

                                     TATGACAAAGAGAAAAAAAAAAAAGAGAAAAAAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCA                                                      <  SRR4302537.5704412/94‑1 (MQ=60)

                                       agaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaacccCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAG                                                     <  SRR4302537.3449070/57‑1 (MQ=60)

                                       TGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGC                                                    <  SRR4302537.5970137/94‑1 (MQ=60)

                                       TGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGC                                                    >  SRR4302537.6378273/1‑94 (MQ=60)

                                       TGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGC                                                    <  SRR4302537.4820920/94‑1 (MQ=60)

                                        GACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCT                                                   <  SRR4302537.766882/94‑1 (MQ=60)

                                         ACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTG                                                  >  SRR4302537.4684755/1‑94 (MQ=60)

                                         ACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTG                                                  >  SRR4302537.4054839/1‑94 (MQ=60)

                                            CAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAA                                               >  SRR4302537.1459770/1‑94 (MQ=60)

                                             AGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAA                                              >  SRR4302537.3912843/1‑94 (MQ=60)

                                                    AAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCA                                       >  SRR4302537.4784652/1‑94 (MQ=60)

                                                      AGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATT                                     >  SRR4302537.3951889/1‑94 (MQ=60)

                                                       GAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTT                                    >  SRR4302537.2696265/1‑94 (MQ=60)

                                                        AAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTC                                   >  SRR4302537.2145156/1‑94 (MQ=60)

                                                           AAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGGGGACGTACCCGAGAGGAAATAACGCAGACCCAGCTGGAAAAacagaacatcaac                                >  SRR4302537.1012428/1‑81 (MQ=60)

                                                           AAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGC                                >  SRR4302537.388901/1‑94 (MQ=60)

                                                           AAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGC                                <  SRR4302537.3045271/94‑1 (MQ=60)

                                                           AAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGT                                >  SRR4302537.4267319/1‑94 (MQ=60)

                                                           AAGAGAAAAAAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGC                                <  SRR4302537.3590119/94‑1 (MQ=60)

                                                            AGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCT                               >  SRR4302537.2357790/1‑94 (MQ=60)

                                                            AGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCT                               <  SRR4302537.2103304/94‑1 (MQ=60)

                                                              AGAAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTC                             >  SRR4302537.1619440/1‑94 (MQ=60)

                                                                AAAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGC                           >  SRR4302537.3535406/1‑94 (MQ=60)

                                                                AAAAAAAAACCGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCG                            <  SRR4302537.5520947/94‑1 (MQ=60)

                                                                 AAAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCA                          >  SRR4302537.2750134/1‑94 (MQ=60)

                                                                  AAGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAG                         <  SRR4302537.736562/94‑1 (MQ=60)

                                                                   aaaacA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGG                        <  SRR4302537.4482538/89‑1 (MQ=60)

                                                                   AGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGG                        >  SRR4302537.4515281/1‑94 (MQ=60)

                                                                   AGAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGG                        <  SRR4302537.2140858/94‑1 (MQ=60)

                                                                    GAAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGC                       >  SRR4302537.4606866/1‑94 (MQ=60)

                                                                     AAAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCA                      <  SRR4302537.6470248/94‑1 (MQ=60)

                                                                      AAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAA                     >  SRR4302537.1201536/1‑94 (MQ=60)

                                                                      AAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAA                     >  SRR4302537.2073459/1‑94 (MQ=60)

                                                                      AAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAA                     >  SRR4302537.1871887/1‑94 (MQ=60)

                                                                      AAA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAA                     <  SRR4302537.3280687/94‑1 (MQ=60)

                                                                       AA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGGTTCGCAGGCAAA                    >  SRR4302537.1437157/1‑94 (MQ=60)

                                                                       AA‑CGCCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAA                    <  SRR4302537.3878866/94‑1 (MQ=60)

                                                                          CGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAG                  <  SRR4302537.4479697/94‑1 (MQ=60)

                                                                            CCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTG                <  SRR4302537.5034012/94‑1 (MQ=60)

                                                                            CCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTG                <  SRR4302537.3609990/94‑1 (MQ=60)

                                                                            CCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTG                >  SRR4302537.4346960/1‑94 (MQ=60)

                                                                            CCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTG                >  SRR4302537.5644481/1‑94 (MQ=60)

                                                                            CCCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCACGCAAAAGTG                >  SRR4302537.1634981/1‑94 (MQ=60)

                                                                             CCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGA               >  SRR4302537.5778920/1‑94 (MQ=60)

                                                                             CCCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGA               >  SRR4302537.4258507/1‑94 (MQ=60)

                                                                              CCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAA              >  SRR4302537.753475/1‑94 (MQ=60)

                                                                              CCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAA              <  SRR4302537.6222515/94‑1 (MQ=60)

                                                                              CCTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAA              >  SRR4302537.4682108/1‑94 (MQ=60)

                                                                               CTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAA             <  SRR4302537.4232967/94‑1 (MQ=60)

                                                                               CTGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAA             <  SRR4302537.1267251/94‑1 (MQ=60)

                                                                                TGCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAT            <  SRR4302537.3515827/94‑1 (MQ=60)

                                                                                 GCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATC           >  SRR4302537.51283/1‑94 (MQ=60)

                                                                                 GCCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTAGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATC           <  SRR4302537.4355879/94‑1 (MQ=60)

                                                                                  CCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCG          <  SRR4302537.2717302/94‑1 (MQ=60)

                                                                                  CCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCG          >  SRR4302537.3057877/1‑94 (MQ=60)

                                                                                  CCAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCG          >  SRR4302537.1534457/1‑94 (MQ=60)

                                                                                   CAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGA         >  SRR4302537.3919041/1‑94 (MQ=60)

                                                                                   CAGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGA         >  SRR4302537.6473968/1‑94 (MQ=60)

                                                                                    AGCAGCTGGCGGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGAT        <  SRR4302537.3365726/94‑1 (MQ=60)

                                                                                    AGCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGAT        >  SRR4302537.5110016/1‑94 (MQ=60)

                                                                                     GCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATA       <  SRR4302537.3145531/94‑1 (MQ=60)

                                                                                     GCAACTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATA       <  SRR4302537.5217154/94‑1 (MQ=60)

                                                                                         CTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCA   >  SRR4302537.5892613/1‑94 (MQ=60)

                                                                                          TGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAG  <  SRR4302537.2569690/94‑1 (MQ=60)

TACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAA‑CGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAG  >  NC_000913/2458553‑2458735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`