Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,466,614 A→G G127G (GGA→GGG)  b2351 → putative glycan biosynthesis enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,466,6140AG93.9% 806.5 / 30.4 264G127G (GGA→GGG) b2351putative glycan biosynthesis enzyme
Reads supporting (aligned to +/- strand):  ref base A (5/11);  new base G (130/118);  total (135/129)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 273 total aligned reads displayed.

CCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCA  >  NC_000913/2466524‑2466702
                                                                                          |                                                                                        
cccGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCg                                                                                        >  2:3328599/1‑93 (MQ=255)
cccGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCg                                                                                        >  2:4368965/1‑93 (MQ=255)
 ccGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGgcgc                                                                                       >  2:3574974/1‑93 (MQ=255)
     ttGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGa                                                                                   >  2:1761997/1‑93 (MQ=255)
     ttGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGGGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGGCTGa                                                                                   >  2:2785728/1‑93 (MQ=255)
       gAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAg                                                                                 <  1:2129821/93‑1 (MQ=255)
         ggTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCg                                                                               >  1:4265167/1‑93 (MQ=255)
         ggTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCg                                                                               >  2:4184634/1‑93 (MQ=255)
          gTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTAAACTGATGGGCGCCTGAAGCGa                                                                              >  1:3384965/1‑93 (MQ=255)
           ttATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGaa                                                                             >  2:5588326/1‑93 (MQ=255)
             aTTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGaaaa                                                                           <  1:4572387/93‑1 (MQ=255)
             aTTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGaaaa                                                                           >  2:6103782/1‑93 (MQ=255)
             aTTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGaaaa                                                                           >  1:2000178/1‑93 (MQ=255)
              ttCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGaaaaa                                                                          >  2:159312/1‑93 (MQ=255)
              ttCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGaaaaa                                                                          <  1:2477459/93‑1 (MQ=255)
                ccTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACg                                                                        >  1:2375872/1‑93 (MQ=255)
                ccTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACg                                                                        <  2:5217667/93‑1 (MQ=255)
                 cTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGGAAAACgg                                                                       >  2:4562505/1‑93 (MQ=255)
                  tCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGc                                                                      <  1:5029417/93‑1 (MQ=255)
                  tCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGc                                                                      <  2:5142265/93‑1 (MQ=255)
                   cATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCt                                                                     >  2:4891632/1‑93 (MQ=255)
                      cTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAg                                                                  <  2:3000373/93‑1 (MQ=255)
                        tATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCCACTGATGGGCGCCTGAAGCGAAACACGGCTGCGTg                                                                >  2:1178613/1‑93 (MQ=255)
                        tATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTg                                                                <  2:1885337/93‑1 (MQ=255)
                         aTTGAAAAATGGCAAGCAGGTTCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTgg                                                               <  2:4023474/93‑1 (MQ=255)
                         aTTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTgg                                                               >  1:1826211/1‑93 (MQ=255)
                         aTTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTgg                                                               <  1:2605164/93‑1 (MQ=255)
                           tGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGtt                                                             <  1:1118913/93‑1 (MQ=255)
                            gAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTc                                                            <  2:2071979/93‑1 (MQ=255)
                            gAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTc                                                            <  1:1450068/93‑1 (MQ=255)
                              aaaaTGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCta                                                          >  2:6461044/1‑93 (MQ=255)
                               aaaTGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCtat                                                         <  1:4816058/93‑1 (MQ=255)
                               aaaTGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCtat                                                         <  1:5553119/93‑1 (MQ=255)
                               aaaTGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCtat                                                         <  1:5783223/93‑1 (MQ=255)
                                aaTGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCtata                                                        >  1:6249256/1‑93 (MQ=255)
                                 aTGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATaa                                                       >  2:4990873/1‑93 (MQ=255)
                                 aTGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATaa                                                       <  2:5979821/93‑1 (MQ=255)
                                 aTGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATaa                                                       <  1:1362654/93‑1 (MQ=255)
                                 aTGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATaa                                                       <  1:3566588/93‑1 (MQ=255)
                                   ggCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGc                                                     <  1:108641/93‑1 (MQ=255)
                                      aaGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGTTcc                                                  >  2:384707/1‑93 (MQ=255)
                                      aaGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTcc                                                  <  1:439557/93‑1 (MQ=255)
                                      aaGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTcc                                                  <  1:3721533/93‑1 (MQ=255)
                                      aaGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTcc                                                  >  2:657748/1‑93 (MQ=255)
                                       aGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCca                                                 <  2:1446114/93‑1 (MQ=255)
                                           ggTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaat                                             >  2:3444045/1‑93 (MQ=255)
                                           ggTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaat                                             <  2:5637672/93‑1 (MQ=255)
                                            gTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaata                                            <  1:2837992/93‑1 (MQ=255)
                                             tGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaataa                                           <  2:5878648/93‑1 (MQ=255)
                                             tGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaataa                                           >  2:2593771/1‑93 (MQ=255)
                                             tGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaataa                                           <  1:1247661/93‑1 (MQ=255)
                                              gCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaataaa                                          <  2:4156563/93‑1 (MQ=255)
                                              gCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaataaa                                          <  2:2746711/93‑1 (MQ=255)
                                                tGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaataaaat                                        <  2:1267749/93‑1 (MQ=255)
                                                tGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaataaaat                                        >  1:4133178/1‑93 (MQ=255)
                                                tGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaataaaat                                        >  2:5324683/1‑93 (MQ=255)
                                                tGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaataaaat                                        >  2:2115802/1‑93 (MQ=255)
                                                  atatGGTTCTTGCTAAAAGATCTGACCGCTCAGCTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaataaaataa                                      >  1:4647416/1‑93 (MQ=255)
                                                  atatGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACaataaaataa                                      <  1:3837811/93‑1 (MQ=255)
                                                    atGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGc                                    >  2:198238/1‑93 (MQ=255)
                                                    atGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGc                                    >  2:2695038/1‑93 (MQ=255)
                                                    atGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGc                                    <  1:751331/93‑1 (MQ=255)
                                                    atGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGc                                    <  1:5935790/93‑1 (MQ=255)
                                                     tGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCa                                   <  2:730907/93‑1 (MQ=255)
                                                     tGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCa                                   >  2:6055690/1‑93 (MQ=255)
                                                     tGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCa                                   >  1:3775184/1‑93 (MQ=255)
                                                       gTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAAt                                 >  2:1054595/1‑93 (MQ=255)
                                                       gTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAAt                                 >  2:4590437/1‑93 (MQ=255)
                                                        ttCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATc                                >  1:4481696/1‑93 (MQ=255)
                                                        ttCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATc                                <  1:1507352/93‑1 (MQ=255)
                                                         tCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAGGCGAAAAACGGCTGAGTGATTCTATAAGCTCCACAATAAAATAAGCAATcc                               <  1:3315610/93‑1 (MQ=255)
                                                         tCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATcc                               <  1:3142305/93‑1 (MQ=255)
                                                         tCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATcc                               >  1:5235041/1‑93 (MQ=255)
                                                           ttGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTa                             >  2:6179367/1‑93 (MQ=255)
                                                            tGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTaa                            <  2:4676106/93‑1 (MQ=255)
                                                            tGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTaa                            <  1:5456846/93‑1 (MQ=255)
                                                                aaaaGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAAtt                        >  1:3482146/1‑93 (MQ=255)
                                                                aaaaGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAAtt                        >  2:2005877/1‑93 (MQ=255)
                                                                aaaaGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAAtt                        >  1:635936/1‑93 (MQ=255)
                                                                aaaaGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAAtt                        >  1:683568/1‑93 (MQ=255)
                                                                  aaGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGa                      >  2:475555/1‑93 (MQ=255)
                                                                     aTCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAaga                   <  1:2530967/93‑1 (MQ=255)
                                                                      tCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAagag                  <  2:2462527/93‑1 (MQ=255)
                                                                        tGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGaa                <  1:5675843/93‑1 (MQ=255)
                                                                        tGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGGGGTTCTATAAGCTCCACAATAAAATAAGCCATCCTAACATTGAAGAGaa                >  2:3022818/1‑93 (MQ=255)
                                                                         gACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAAt               <  1:5441223/93‑1 (MQ=255)
                                                                         gACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAAt               >  2:5799830/1‑93 (MQ=255)
                                                                            cGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGtt            >  2:231265/1‑93 (MQ=255)
                                                                            cGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGtt            >  2:1405046/1‑93 (MQ=255)
                                                                            cGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGGTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGtt            >  2:511854/1‑93 (MQ=255)
                                                                            cGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGtt            <  2:2890742/93‑1 (MQ=255)
                                                                                cAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGGGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTg        >  1:3050108/1‑93 (MQ=255)
                                                                                 aaCTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGa       <  1:1627424/93‑1 (MQ=255)
                                                                                  aCTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGAt      >  2:172496/1‑93 (MQ=255)
                                                                                   cTGATGGGCGCCTGAAGCGAAAAGCGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGAtt     <  2:5500699/93‑1 (MQ=255)
                                                                                   cTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGAtt     <  2:3667493/93‑1 (MQ=255)
                                                                                    tGATGGGCGCGTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGAttt    <  2:3188104/93‑1 (MQ=255)
                                                                                     gATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTc   <  1:2891676/93‑1 (MQ=255)
                                                                                     gATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTc   <  1:6298544/93‑1 (MQ=255)
                                                                                      aTGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCa  <  2:813308/93‑1 (MQ=255)
                                                                                          |                                                                                        
CCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCA  >  NC_000913/2466524‑2466702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTG  >  NC_000913/2466521‑2466707
                                                                                             |                                                                                             
AGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGG                                                                                               >  SRR4302537.726393/1‑94 (MQ=60)
AGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGG                                                                                               <  SRR4302537.3596900/94‑1 (MQ=60)
 GACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGC                                                                                              <  SRR4302537.2444684/94‑1 (MQ=60)
  ACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCG                                                                                             <  SRR4302537.5329483/94‑1 (MQ=60)
   CCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGC                                                                                            >  SRR4302537.6206764/1‑94 (MQ=60)
   CCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGC                                                                                            >  SRR4302537.5675315/1‑94 (MQ=60)
   CCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGC                                                                                            >  SRR4302537.3842755/1‑94 (MQ=60)
     CGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCT                                                                                          >  SRR4302537.2580470/1‑94 (MQ=60)
       ATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGA                                                                                        <  SRR4302537.5016040/94‑1 (MQ=60)
        TTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAA                                                                                       >  SRR4302537.5798111/1‑94 (MQ=60)
          GAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGC                                                                                     <  SRR4302537.2144805/94‑1 (MQ=60)
          GAGGTTATTCCTCATCTTATTGAAAAAAGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGC                                                                                     <  SRR4302537.3453619/94‑1 (MQ=60)
           AGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCG                                                                                    >  SRR4302537.4294803/1‑94 (MQ=60)
           AGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCG                                                                                    <  SRR4302537.3777925/94‑1 (MQ=60)
           AGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCG                                                                                    >  SRR4302537.1103885/1‑94 (MQ=60)
            GGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGA                                                                                   >  SRR4302537.6481416/1‑94 (MQ=60)
            GGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTAAACTGATGGGCGCCTGAAGCGA                                                                                   >  SRR4302537.3408948/1‑94 (MQ=60)
               TATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAA                                                                                >  SRR4302537.2014086/1‑94 (MQ=60)
                ATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAA                                                                               <  SRR4302537.4604039/94‑1 (MQ=60)
                 TTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAAC                                                                              <  SRR4302537.2494876/94‑1 (MQ=60)
                 TTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAAC                                                                              <  SRR4302537.3126049/94‑1 (MQ=60)
                 TTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAAC                                                                              <  SRR4302537.2860084/94‑1 (MQ=60)
                 TTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAAC                                                                              >  SRR4302537.4466403/1‑94 (MQ=60)
                 TTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAAC                                                                              <  SRR4302537.4514760/94‑1 (MQ=60)
                  TCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACG                                                                             >  SRR4302537.2392612/1‑94 (MQ=60)
                    CTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGC                                                                           >  SRR4302537.4648285/1‑94 (MQ=60)
                     TCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCT                                                                          <  SRR4302537.5064041/94‑1 (MQ=60)
                       ATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGA                                                                        >  SRR4302537.3271620/1‑94 (MQ=60)
                       ATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTAGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGA                                                                        >  SRR4302537.4699044/1‑94 (MQ=60)
                        TCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAG                                                                       <  SRR4302537.5559353/94‑1 (MQ=60)
                         CTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGT                                                                      <  SRR4302537.1211813/94‑1 (MQ=60)
                          TTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTG                                                                     <  SRR4302537.292197/94‑1 (MQ=60)
                          TTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTG                                                                     <  SRR4302537.3596514/94‑1 (MQ=60)
                           TATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGG                                                                    >  SRR4302537.1838938/1‑94 (MQ=60)
                           TATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGG                                                                    >  SRR4302537.6434780/1‑94 (MQ=60)
                            ATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGT                                                                   <  SRR4302537.2460203/94‑1 (MQ=60)
                            ATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGT                                                                   <  SRR4302537.2623379/94‑1 (MQ=60)
                             TTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTT                                                                  <  SRR4302537.4129431/94‑1 (MQ=60)
                              TGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTC                                                                 <  SRR4302537.1126659/94‑1 (MQ=60)
                               GAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCT                                                                >  SRR4302537.4406902/1‑94 (MQ=60)
                               GAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCT                                                                <  SRR4302537.828510/94‑1 (MQ=60)
                               GAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCT                                                                <  SRR4302537.2342793/94‑1 (MQ=60)
                               GAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCT                                                                <  SRR4302537.1460171/94‑1 (MQ=60)
                                  AAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATA                                                             <  SRR4302537.4849299/94‑1 (MQ=60)
                                  AAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATA                                                             <  SRR4302537.5591038/94‑1 (MQ=60)
                                  AAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATA                                                             <  SRR4302537.5822868/94‑1 (MQ=60)
                                  AAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATA                                                             >  SRR4302537.6292188/1‑94 (MQ=60)
                                   AATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATATGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGAGGTTCTttct                                                            >  SRR4302537.1485482/1‑90 (MQ=60)
                                    ATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAG                                                           <  SRR4302537.1372147/94‑1 (MQ=60)
                                    ATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAG                                                           <  SRR4302537.2371192/94‑1 (MQ=60)
                                    ATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAG                                                           >  SRR4302537.2534761/1‑94 (MQ=60)
                                    ATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAG                                                           >  SRR4302537.4692656/1‑94 (MQ=60)
                                    ATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAG                                                           <  SRR4302537.3591738/94‑1 (MQ=60)
                                     TGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGC                                                          >  SRR4302537.5478717/1‑94 (MQ=60)
                                      GGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCT                                                         <  SRR4302537.109436/94‑1 (MQ=60)
                                         AAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCA                                                      <  SRR4302537.442613/94‑1 (MQ=60)
                                         AAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCA                                                      <  SRR4302537.3747679/94‑1 (MQ=60)
                                         AAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCA                                                      >  SRR4302537.5663129/1‑94 (MQ=60)
                                          AGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCAC                                                     >  SRR4302537.5371970/1‑94 (MQ=60)
                                           GCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACA                                                    <  SRR4302537.1320741/94‑1 (MQ=60)
                                             AGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAAT                                                  >  SRR4302537.2437201/1‑94 (MQ=60)
                                              GGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATA                                                 >  SRR4302537.5172482/1‑94 (MQ=60)
                                               GTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAA                                                <  SRR4302537.2857848/94‑1 (MQ=60)
                                               GTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAA                                                <  SRR4302537.1673353/94‑1 (MQ=60)
                                                TGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAA                                               >  SRR4302537.3161775/1‑94 (MQ=60)
                                                TGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAA                                               >  SRR4302537.4604021/1‑94 (MQ=60)
                                                TGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAA                                               <  SRR4302537.1256351/94‑1 (MQ=60)
                                                  CTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAAT                                             >  SRR4302537.4162021/1‑94 (MQ=60)
                                                   TGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATA                                            >  SRR4302537.3861348/1‑94 (MQ=60)
                                                    GATATGGTTCTTGCTAAAAGATCTGACCGCTCAGCTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAA                                           >  SRR4302537.4679547/1‑94 (MQ=60)
                                                    GATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAA                                           >  SRR4302537.2057288/1‑94 (MQ=60)
                                                     ATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAG                                          <  SRR4302537.3864697/94‑1 (MQ=60)
                                                       ATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCA                                        >  SRR4302537.3801691/1‑94 (MQ=60)
                                                       ATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCA                                        <  SRR4302537.756454/94‑1 (MQ=60)
                                                       ATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCA                                        <  SRR4302537.5976524/94‑1 (MQ=60)
                                                       ATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCA                                        <  SRR4302537.5126021/94‑1 (MQ=60)
                                                       ATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCA                                        <  SRR4302537.1513778/94‑1 (MQ=60)
                                                        TGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAA                                       >  SRR4302537.4891490/1‑94 (MQ=60)
                                                          GTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATC                                     >  SRR4302537.4512697/1‑94 (MQ=60)
                                                          GTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATC                                     >  SRR4302537.3006703/1‑94 (MQ=60)
                                                           TTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCC                                    >  SRR4302537.1970244/1‑94 (MQ=60)
                                                           TTCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCC                                    >  SRR4302537.5271057/1‑94 (MQ=60)
                                                           TTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCC                                    <  SRR4302537.1517869/94‑1 (MQ=60)
                                                            TCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAGGCGAAAAACGGCTGAGTGATTCTATAAGCTCCACAATAAAATAAGCAATCCT                                   <  SRR4302537.3339063/94‑1 (MQ=60)
                                                            TCTTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCT                                   <  SRR4302537.3164359/94‑1 (MQ=60)
                                                              TTGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAA                                 <  SRR4302537.5869706/94‑1 (MQ=60)
                                                               TGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAA                                <  SRR4302537.4017605/94‑1 (MQ=60)
                                                               TGCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAA                                <  SRR4302537.5494065/94‑1 (MQ=60)
                                                                GCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAA                               >  SRR4302537.6376967/1‑94 (MQ=60)
                                                                GCTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAA                               <  SRR4302537.4328986/94‑1 (MQ=60)
                                                                 CTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAAT                              >  SRR4302537.1557168/1‑94 (MQ=60)
                                                                 CTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAAT                              <  SRR4302537.3291482/94‑1 (MQ=60)
                                                                 CTAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAAT                              >  SRR4302537.6245368/1‑94 (MQ=60)
                                                                  TAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATT                             >  SRR4302537.688255/1‑94 (MQ=60)
                                                                  TAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATT                             >  SRR4302537.640261/1‑94 (MQ=60)
                                                                  TAAAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATT                             >  SRR4302537.3506784/1‑94 (MQ=60)
                                                                    AAAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCTACAATAAAATAAGCAATCCTAAAATTGA                           >  SRR4302537.737787/1‑94 (MQ=60)
                                                                     AAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAA                          >  SRR4302537.1445182/1‑94 (MQ=60)
                                                                     AAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAA                          <  SRR4302537.1628350/94‑1 (MQ=60)
                                                                     AAGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAA                          >  SRR4302537.4471090/1‑94 (MQ=60)
                                                                      AGATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAG                         <  SRR4302537.745476/94‑1 (MQ=60)
                                                                      AGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAG                         <  SRR4302537.2612294/94‑1 (MQ=60)
                                                                      AGATCTGACAGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAG                         <  SRR4302537.3352983/94‑1 (MQ=60)
                                                                       GATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGA                        >  SRR4302537.2090266/1‑94 (MQ=60)
                                                                       GATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGA                        <  SRR4302537.3416160/94‑1 (MQ=60)
                                                                        ATCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAG                       <  SRR4302537.2548726/94‑1 (MQ=60)
                                                                         TCTGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGA                      <  SRR4302537.1318740/94‑1 (MQ=60)
                                                                           TGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAAT                    >  SRR4302537.1792093/1‑94 (MQ=60)
                                                                           TGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAAT                    <  SRR4302537.5714679/94‑1 (MQ=60)
                                                                           TGACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAAT                    >  SRR4302537.4186613/1‑94 (MQ=60)
                                                                            GACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATG                   <  SRR4302537.5478341/94‑1 (MQ=60)
                                                                             ACCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGT                  <  SRR4302537.3576057/94‑1 (MQ=60)
                                                                              CCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTT                 <  SRR4302537.5857916/94‑1 (MQ=60)
                                                                              CCGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTT                 <  SRR4302537.630373/94‑1 (MQ=60)
                                                                               CGCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTG                <  SRR4302537.2849284/94‑1 (MQ=60)
                                                                                GCTCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGG               <  SRR4302537.3695074/94‑1 (MQ=60)
                                                                                  TCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTG             <  SRR4302537.112910/94‑1 (MQ=60)
                                                                                  TCAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGGGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTG             >  SRR4302537.3071359/1‑94 (MQ=60)
                                                                                   CAACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGA            >  SRR4302537.6384734/1‑94 (MQ=60)
                                                                                    AACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGAT           >  SRR4302537.548269/1‑94 (MQ=60)
                                                                                    AACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGAT           <  SRR4302537.1638801/94‑1 (MQ=60)
                                                                                     ACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATT          >  SRR4302537.2635321/1‑94 (MQ=60)
                                                                                     ACTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATT          >  SRR4302537.5552113/1‑94 (MQ=60)
                                                                                      CTGATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTT         >  SRR4302537.3571110/1‑94 (MQ=60)
                                                                                        GATGGGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCA       <  SRR4302537.2911977/94‑1 (MQ=60)
                                                                                        GATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCA       <  SRR4302537.6341684/94‑1 (MQ=60)
                                                                                            GGCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCT   <  SRR4302537.1657487/94‑1 (MQ=60)
                                                                                             GCGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTG  <  SRR4302537.2608168/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTG  >  NC_000913/2466521‑2466707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑