Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,481,129	T→C	R77R (CGA→CGG)	emrK ←	multidrug resistance protein K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,481,129	0	T	C	95.2%	797.3 / 22.1	251	R77R (CGA→CGG)	emrK	multidrug resistance protein K
Reads supporting (aligned to +/- strand): ref base T (4/8); new base C (136/103); total (140/111)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-01

Only 100 of 254 total aligned reads displayed.

TTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGC  >  NC_000913/2481039‑2481218

ttGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCg                                                                                         >  2:6065227/1‑93 (MQ=255)

  gCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGaa                                                                                       <  2:4982316/93‑1 (MQ=255)

     gAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGt                                                                                    <  1:3607395/93‑1 (MQ=255)

     gAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGt                                                                                    >  1:4775337/1‑93 (MQ=255)

     gAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGt                                                                                    >  2:4645487/1‑93 (MQ=255)

     gAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGt                                                                                    >  2:1638339/1‑93 (MQ=255)

     gAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGt                                                                                    >  1:261314/1‑93 (MQ=255)

     gAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGt                                                                                    <  2:4125934/93‑1 (MQ=255)

      aaCAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTa                                                                                   >  2:4018043/1‑93 (MQ=255)

      aaCAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTa                                                                                   >  2:129102/1‑93 (MQ=255)

      aaCAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTa                                                                                   >  2:6004070/1‑93 (MQ=255)

       aCAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAg                                                                                  <  1:6148741/93‑1 (MQ=255)

       aCAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAg                                                                                  >  1:1892441/1‑93 (MQ=255)

        cAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGt                                                                                 <  1:3859504/93‑1 (MQ=255)

         aaTATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGtt                                                                                >  1:2688897/1‑93 (MQ=255)

         aaTATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGtt                                                                                <  1:6342358/93‑1 (MQ=255)

         aaTATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGtt                                                                                <  2:1847862/93‑1 (MQ=255)

         aaTATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGtt                                                                                <  2:3248057/93‑1 (MQ=255)

          atatTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTc                                                                               <  1:5238534/93‑1 (MQ=255)

           tatTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCg                                                                              <  2:2021078/93‑1 (MQ=255)

           tatTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCg                                                                              >  1:4339421/1‑93 (MQ=255)

            atTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGt                                                                             <  1:2429057/93‑1 (MQ=255)

            atTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGt                                                                             <  2:1045296/93‑1 (MQ=255)

             tttGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTa                                                                            >  1:1504313/1‑93 (MQ=255)

             tttGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTa                                                                            <  1:5588638/93‑1 (MQ=255)

             tttGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTa                                                                            <  1:3949363/93‑1 (MQ=255)

              ttGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTAt                                                                           >  2:1708924/1‑93 (MQ=255)

                gCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCt                                                                         >  1:5466796/1‑93 (MQ=255)

                gCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCt                                                                         >  1:5317917/1‑93 (MQ=255)

                gCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGCCACTTTGCCGAACGTAGTTCGTATCt                                                                         >  1:4318278/1‑93 (MQ=255)

                 ccAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCtt                                                                        <  1:2899635/93‑1 (MQ=255)

                 ccAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCtt                                                                        <  2:4854575/93‑1 (MQ=255)

                   aGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTa                                                                      <  1:3670513/93‑1 (MQ=255)

                     attatgTTTAGCTTTATTGAGTGCGATAGTAGCATCAGTTTTATCCAGTGAAACTAAAATGTCCCCTTGCCGAACGTAGTTCGTATCTTTATg                                                                    <  1:981850/93‑1 (MQ=255)

                      ttattTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGa                                                                   >  1:674034/1‑93 (MQ=255)

                      ttattTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGa                                                                   >  1:2985994/1‑93 (MQ=255)

                      ttattTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCATGCCGAACGTAGTTCGTATCTTTATGa                                                                   >  2:152159/1‑93 (MQ=255)

                       tattTTTAGCTTTATTGAGTGCGATAGTGGCACCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGAt                                                                  >  1:799591/1‑93 (MQ=255)

                         tttttAGCTTTATTGAGTGCTATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTa                                                                <  2:3308513/93‑1 (MQ=255)

                         tttttAGCTTTATTGAGTGCGATGGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTa                                                                <  1:4110445/93‑1 (MQ=255)

                         tttttAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTa                                                                >  2:6213795/1‑93 (MQ=255)

                         tttttAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTa                                                                >  1:2757161/1‑93 (MQ=255)

                         tttttAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTa                                                                <  1:3153342/93‑1 (MQ=255)

                          ttttAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAACCTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTaa                                                               <  1:4187305/93‑1 (MQ=255)

                          ttttAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTaa                                                               <  1:5119293/93‑1 (MQ=255)

                          ttttAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTaa                                                               <  1:3435297/93‑1 (MQ=255)

                           tttAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAAc                                                              >  1:5895934/1‑93 (MQ=255)

                           tttAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAAc                                                              >  1:6079720/1‑93 (MQ=255)

                            ttAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACg                                                             >  1:3215527/1‑93 (MQ=255)

                               gCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACa                                                          <  2:5350016/93‑1 (MQ=255)

                                 tttATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGt                                                        <  1:1003269/93‑1 (MQ=255)

                                 tttATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGt                                                        <  1:1095818/93‑1 (MQ=255)

                                 tttATTGAGGGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGt                                                        <  1:1171083/93‑1 (MQ=255)

                                   tATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGa                                                      <  1:1347621/93‑1 (MQ=255)

                                    aTTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGac                                                     >  2:2323250/1‑93 (MQ=255)

                                    aTTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGac                                                     >  2:2576217/1‑93 (MQ=255)

                                     ttGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGaca                                                    >  2:1439285/1‑93 (MQ=255)

                                     ttGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGaca                                                    >  1:2932712/1‑93 (MQ=255)

                                      tGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGacac                                                   >  1:2499886/1‑93 (MQ=255)

                                      tGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGacac                                                   <  1:2202389/93‑1 (MQ=255)

                                         gTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTAc                                                >  2:5451240/1‑93 (MQ=255)

                                          tGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTAcc                                               <  1:312229/93‑1 (MQ=255)

                                          tGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTAcc                                               >  1:1662430/1‑93 (MQ=255)

                                             gATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTga                                            >  2:4196473/1‑93 (MQ=255)

                                             gATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTga                                            >  1:2063351/1‑93 (MQ=255)

                                                 gTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACt                                        <  2:1457956/93‑1 (MQ=255)

                                                 gTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACt                                        <  2:5309551/93‑1 (MQ=255)

                                                       tCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCa                                  <  1:1038966/93‑1 (MQ=255)

                                                        cAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAg                                 <  2:5946998/93‑1 (MQ=255)

                                                         aGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGa                                <  2:6168692/93‑1 (MQ=255)

                                                         aGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGa                                >  2:1548696/1‑93 (MQ=255)

                                                          gTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGaa                               <  2:3609563/93‑1 (MQ=255)

                                                          gTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGaa                               >  2:5154582/1‑93 (MQ=255)

                                                               aTCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTgg                          >  2:3766570/1‑93 (MQ=255)

                                                               aTCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTgg                          >  1:4064474/1‑93 (MQ=255)

                                                                tCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGa                         >  1:5558411/1‑93 (MQ=255)

                                                                   aGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCt                      >  1:492363/1‑93 (MQ=255)

                                                                    gTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTg                     <  2:4277890/93‑1 (MQ=255)

                                                                     tGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGc                    >  1:1771100/1‑93 (MQ=255)

                                                                     tGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGc                    >  1:915309/1‑93 (MQ=255)

                                                                      gAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCa                   >  2:5855330/1‑93 (MQ=255)

                                                                       aaaCTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCAt                  <  2:332399/93‑1 (MQ=255)

                                                                       aaaCTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCAt                  <  1:3195938/93‑1 (MQ=255)

                                                                        aaCTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCAtt                 <  2:2766081/93‑1 (MQ=255)

                                                                         aCTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTc                <  2:4301451/93‑1 (MQ=255)

                                                                          cTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTcc               >  2:562607/1‑93 (MQ=255)

                                                                           tAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTccc              >  2:5694023/1‑93 (MQ=255)

                                                                           tAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTccc              >  1:2287392/1‑93 (MQ=255)

                                                                              aaTGTCACCTTGCCGAACGAAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCCGAAATTAGATCTGCGTTCCCCga           >  2:6293610/1‑92 (MQ=255)

                                                                               aTGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTg          >  2:47249/1‑93 (MQ=255)

                                                                                tGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGa         <  2:2494105/93‑1 (MQ=255)

                                                                                   cACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACAt      <  1:5783108/93‑1 (MQ=255)

                                                                                    aCCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATa     >  1:5937912/1‑93 (MQ=255)

                                                                                    aCCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATa     <  2:382391/93‑1 (MQ=255)

                                                                                    aCCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATa     <  1:2147237/93‑1 (MQ=255)

                                                                                    aCCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATa     <  2:3052405/93‑1 (MQ=255)

                                                                                    aCCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATa     <  2:903402/93‑1 (MQ=255)

                                                                                      cTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAgg   >  2:4823695/1‑93 (MQ=255)

                                                                                      cTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAgg   <  2:6437409/93‑1 (MQ=255)

                                                                                       ttGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGc  >  1:3757811/1‑93 (MQ=255)

TTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGC  >  NC_000913/2481039‑2481218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCA  >  NC_000913/2481036‑2481222

CGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGC                                                                                               >  SRR4302537.4410090/1‑94 (MQ=60)

CGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGC                                                                                               <  SRR4302537.2766862/94‑1 (MQ=60)

CGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGC                                                                                               >  SRR4302537.4680943/1‑94 (MQ=60)

 GTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCC                                                                                              >  SRR4302537.4866444/1‑94 (MQ=60)

 GTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCC                                                                                              >  SRR4302537.4323726/1‑94 (MQ=60)

 GTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCC                                                                                              >  SRR4302537.1388724/1‑94 (MQ=60)

   TTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGA                                                                                            <  SRR4302537.3182953/94‑1 (MQ=60)

      CCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACG                                                                                         >  SRR4302537.1263982/1‑94 (MQ=60)

      CCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACG                                                                                         >  SRR4302537.1489378/1‑94 (MQ=60)

      CCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACG                                                                                         >  SRR4302537.1729861/1‑94 (MQ=60)

      CCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACG                                                                                         <  SRR4302537.5788175/94‑1 (MQ=60)

       CGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGT                                                                                        >  SRR4302537.263156/1‑94 (MQ=60)

       CGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGT                                                                                        >  SRR4302537.4808304/1‑94 (MQ=60)

       CGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGT                                                                                        >  SRR4302537.562256/1‑94 (MQ=60)

        GAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTA                                                                                       <  SRR4302537.3632819/94‑1 (MQ=60)

        GAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTA                                                                                       >  SRR4302537.2341003/1‑94 (MQ=60)

         AACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAG                                                                                      >  SRR4302537.6510809/1‑94 (MQ=60)

         AACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAG                                                                                      >  SRR4302537.1905594/1‑94 (MQ=60)

          ACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGT                                                                                     <  SRR4302537.6191031/94‑1 (MQ=60)

           CAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTT                                                                                    >  SRR4302537.2707665/1‑94 (MQ=60)

           CAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTT                                                                                    <  SRR4302537.3886523/94‑1 (MQ=60)

           CAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTT                                                                                    >  SRR4302537.2098321/1‑94 (MQ=60)

           CAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTT                                                                                    <  SRR4302537.3341701/94‑1 (MQ=60)

            AATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTC                                                                                   >  SRR4302537.4913133/1‑94 (MQ=60)

            AATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTC                                                                                   <  SRR4302537.6385690/94‑1 (MQ=60)

            AATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTC                                                                                   >  SRR4302537.267955/1‑94 (MQ=60)

             ATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCG                                                                                  >  SRR4302537.4369533/1‑94 (MQ=60)

             ATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCG                                                                                  <  SRR4302537.5274579/94‑1 (MQ=60)

               ATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTA                                                                                >  SRR4302537.1514811/1‑94 (MQ=60)

               ATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTA                                                                                <  SRR4302537.2446174/94‑1 (MQ=60)

               ATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTA                                                                                >  SRR4302537.5230554/1‑94 (MQ=60)

                TTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTAT                                                                               <  SRR4302537.3976986/94‑1 (MQ=60)

                TTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTAT                                                                               <  SRR4302537.3605076/94‑1 (MQ=60)

                TTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTAT                                                                               >  SRR4302537.98040/1‑94 (MQ=60)

                TTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTAT                                                                               <  SRR4302537.5626850/94‑1 (MQ=60)

                TTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTAT                                                                               <  SRR4302537.1821504/94‑1 (MQ=60)

                  TGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCT                                                                             >  SRR4302537.5354427/1‑94 (MQ=60)

                  TGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCT                                                                             >  SRR4302537.5504085/1‑94 (MQ=60)

                  TGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGCCACTTTGCCGAACGTAGTTCGTATCT                                                                             >  SRR4302537.4348295/1‑94 (MQ=60)

                   GCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTT                                                                            >  SRR4302537.6151266/1‑94 (MQ=60)

                   GCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTT                                                                            >  SRR4302537.5967756/1‑94 (MQ=60)

                    CCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTT                                                                           <  SRR4302537.3157065/94‑1 (MQ=60)

                    CCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTT                                                                           <  SRR4302537.2920007/94‑1 (MQ=60)

                     CAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTA                                                                          >  SRR4302537.5310089/1‑94 (MQ=60)

                      AGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTAT                                                                         <  SRR4302537.3696337/94‑1 (MQ=60)

                      AGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTAT                                                                         <  SRR4302537.1039940/94‑1 (MQ=60)

                      AGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTAT                                                                         >  SRR4302537.4059959/1‑94 (MQ=60)

                       GATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATG                                                                        <  SRR4302537.320993/94‑1 (MQ=60)

                        ATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGA                                                                       >  SRR4302537.678649/1‑94 (MQ=60)

                        ATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGA                                                                       >  SRR4302537.3006856/1‑94 (MQ=60)

                        ATTATGTTTAGCTTTATTGAGTGCGATAGTAGCATCAGTTTTATCCAGTGAAACTAAAATGTCCCCTTGCCGAACGTAGTTCGTATCTTTATGA                                                                       <  SRR4302537.988631/94‑1 (MQ=60)

                         TTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGAT                                                                      <  SRR4302537.5898139/94‑1 (MQ=60)

                         TTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGAT                                                                      >  SRR4302537.5606739/1‑94 (MQ=60)

                         TTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGAT                                                                      <  SRR4302537.2231925/94‑1 (MQ=60)

                         TTATTTTTAGCTTTATTGAGTGCGATAGTGGCACCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGAT                                                                      >  SRR4302537.805083/1‑94 (MQ=60)

                          TATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATT                                                                     <  SRR4302537.1076940/94‑1 (MQ=60)

                          TATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATT                                                                     >  SRR4302537.4134107/1‑94 (MQ=60)

                           ATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTA                                                                    >  SRR4302537.37671/1‑94 (MQ=60)

                           ATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTA                                                                    >  SRR4302537.2776405/1‑94 (MQ=60)

                           ATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTA                                                                    >  SRR4302537.4094422/1‑94 (MQ=60)

                            TTTTTAGCTTTATTGAGTGCGATGGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAA                                                                   <  SRR4302537.4139160/94‑1 (MQ=60)

                            TTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAA                                                                   <  SRR4302537.3175483/94‑1 (MQ=60)

                            TTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAA                                                                   >  SRR4302537.2948007/1‑94 (MQ=60)

                             TTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAACCTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAAC                                                                  <  SRR4302537.4216543/94‑1 (MQ=60)

                             TTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAAC                                                                  <  SRR4302537.5154572/94‑1 (MQ=60)

                             TTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAAC                                                                  <  SRR4302537.4361554/94‑1 (MQ=60)

                             TTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAAC                                                                  >  SRR4302537.5936388/1‑94 (MQ=60)

                             TTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAAC                                                                  <  SRR4302537.3459611/94‑1 (MQ=60)

                             TTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAAC                                                                  >  SRR4302537.6121499/1‑94 (MQ=60)

                              TTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACG                                                                 >  SRR4302537.3635926/1‑94 (MQ=60)

                              TTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACG                                                                 >  SRR4302537.3238158/1‑94 (MQ=60)

                               TTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGA                                                                <  SRR4302537.3883691/94‑1 (MQ=60)

                                TAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGAC                                                               >  SRR4302537.1645819/1‑94 (MQ=60)

                                TAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGAC                                                               <  SRR4302537.5000879/94‑1 (MQ=60)

                                  GCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAG                                                             >  SRR4302537.2950078/1‑94 (MQ=60)

                                    TTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTG                                                           >  SRR4302537.393115/1‑94 (MQ=60)

                                    TTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTG                                                           <  SRR4302537.1010194/94‑1 (MQ=60)

                                    TTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTG                                                           <  SRR4302537.1000448/94‑1 (MQ=60)

                                    TTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTG                                                           <  SRR4302537.1103387/94‑1 (MQ=60)

                                    TTTATTGAGGGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTG                                                           <  SRR4302537.1179235/94‑1 (MQ=60)

                                     TTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGA                                                          <  SRR4302537.815867/94‑1 (MQ=60)

                                     TTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGA                                                          >  SRR4302537.688380/1‑94 (MQ=60)

                                      TATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGAC                                                         >  SRR4302537.2623298/1‑94 (MQ=60)

                                      TATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGAC                                                         <  SRR4302537.1357013/94‑1 (MQ=60)

                                       ATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACA                                                        >  SRR4302537.2953305/1‑94 (MQ=60)

                                        TTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACAC                                                       >  SRR4302537.2517478/1‑94 (MQ=60)

                                         TGAGTGCGATAGTTGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGCAGTTCGTATCTTTATGATTAACGACAGTGACACT                                                      <  SRR4302537.6350878/94‑1 (MQ=60)

                                         TGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACT                                                      <  SRR4302537.2217921/94‑1 (MQ=60)

                                         TGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACT                                                      <  SRR4302537.6103981/94‑1 (MQ=60)

                                           AGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTAC                                                    >  SRR4302537.1871994/1‑94 (MQ=60)

                                            GTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACC                                                   >  SRR4302537.1674078/1‑94 (MQ=60)

                                             TGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCT                                                  <  SRR4302537.314387/94‑1 (MQ=60)

                                             TGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCT                                                  <  SRR4302537.5880343/94‑1 (MQ=60)

                                               CGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGA                                                <  SRR4302537.5374111/94‑1 (MQ=60)

                                               CGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGA                                                >  SRR4302537.1270511/1‑94 (MQ=60)

                                               CGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGA                                                >  SRR4302537.2077840/1‑94 (MQ=60)

                                                GATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAG                                               >  SRR4302537.3305431/1‑94 (MQ=60)

                                                 ATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGA                                              >  SRR4302537.1522810/1‑94 (MQ=60)

                                                    GTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTT                                           <  SRR4302537.3968327/94‑1 (MQ=60)

                                                     TGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTG                                          <  SRR4302537.5011351/94‑1 (MQ=60)

                                                      GGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGT                                         >  SRR4302537.2397085/1‑94 (MQ=60)

                                                      GGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCCTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGT                                         <  SRR4302537.1103475/94‑1 (MQ=60)

                                                        CATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGC                                       >  SRR4302537.437443/1‑94 (MQ=60)

                                                         ATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGCTTAACGACAGTGACACTACCTGAGACTTGTGCA                                      >  SRR4302537.2180099/1‑94 (MQ=60)

                                                         ATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCA                                      >  SRR4302537.1094383/1‑94 (MQ=60)

                                                          TCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAG                                     <  SRR4302537.1046116/94‑1 (MQ=60)

                                                           CAGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGA                                    >  SRR4302537.2547570/1‑94 (MQ=60)

                                                            AGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAA                                   >  SRR4302537.3712826/1‑94 (MQ=60)

                                                            AGTTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAA                                   >  SRR4302537.4228602/1‑94 (MQ=60)

                                                              TTTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAT                                 >  SRR4302537.6273563/1‑94 (MQ=60)

                                                               TTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATT                                >  SRR4302537.4263998/1‑94 (MQ=60)

                                                               TTTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATT                                <  SRR4302537.2786031/94‑1 (MQ=60)

                                                                TTATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTG                               >  SRR4302537.3066592/1‑94 (MQ=60)

                                                                 TATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGG                              >  SRR4302537.4092875/1‑94 (MQ=60)

                                                                 TATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGG                              >  SRR4302537.808393/1‑94 (MQ=60)

                                                                  ATCCAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGA                             >  SRR4302537.5596391/1‑94 (MQ=60)

                                                                     CAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCT                          >  SRR4302537.495753/1‑94 (MQ=60)

                                                                     CAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCT                          >  SRR4302537.1929083/1‑94 (MQ=60)

                                                                     CAGTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCT                          <  SRR4302537.6098715/94‑1 (MQ=60)

                                                                       GTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGC                        >  SRR4302537.921630/1‑94 (MQ=60)

                                                                       GTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGC                        <  SRR4302537.4469278/94‑1 (MQ=60)

                                                                       GTGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGC                        >  SRR4302537.1783434/1‑94 (MQ=60)

                                                                        TGAAACTAAATTGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCA                       <  SRR4302537.276344/94‑1 (MQ=60)

                                                                        TGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCA                       <  SRR4302537.226711/94‑1 (MQ=60)

                                                                        TGAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCA                       >  SRR4302537.3092764/1‑94 (MQ=60)

                                                                         GAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCAT                      <  SRR4302537.4168890/94‑1 (MQ=60)

                                                                          AAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATT                     <  SRR4302537.3218411/94‑1 (MQ=60)

                                                                            ACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCC                   >  SRR4302537.352388/1‑94 (MQ=60)

                                                                             CTAAAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCC                  >  SRR4302537.2303526/1‑94 (MQ=60)

                                                                                CAATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGT               <  SRR4302537.5196527/94‑1 (MQ=60)

                                                                                 AATGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTG              >  SRR4302537.212225/1‑94 (MQ=60)

                                                                                   TGTCACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGAC            <  SRR4302537.2764718/94‑1 (MQ=60)

                                                                                      CACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATA         >  SRR4302537.5978660/1‑94 (MQ=60)

                                                                                      CACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATA         <  SRR4302537.5822752/94‑1 (MQ=60)

                                                                                       ACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAG        <  SRR4302537.6397215/94‑1 (MQ=60)

                                                                                       ACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAG        <  SRR4302537.3228733/94‑1 (MQ=60)

                                                                                       ACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAG        >  SRR4302537.5432644/1‑94 (MQ=60)

                                                                                       ACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAG        >  SRR4302537.5552029/1‑94 (MQ=60)

                                                                                       ACCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAG        <  SRR4302537.2162340/94‑1 (MQ=60)

                                                                                        CCTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGG       >  SRR4302537.2493349/1‑94 (MQ=60)

                                                                                         CTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGC      >  SRR4302537.2968403/1‑94 (MQ=60)

                                                                                         CTTGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGC      >  SRR4302537.3784194/1‑94 (MQ=60)

                                                                                           TGCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGT    >  SRR4302537.998350/1‑94 (MQ=60)

                                                                                            GCCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTC   >  SRR4302537.2823159/1‑94 (MQ=60)

                                                                                             CCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCA  >  SRR4302537.4102336/1‑94 (MQ=60)

CGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCA  >  NC_000913/2481036‑2481222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`