Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,516,832	T→C	intergenic (+1/‑1)	yfeC → / → yfeD	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,516,832	0	T	C	99.6%	830.0 / NA	233	intergenic (+1/‑1)	yfeC/yfeD	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (112/120); minor base A (1/0); total (113/120)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

CCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGA  >  NC_000913/2516742‑2516921

ccAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAacat                                                                                         <  2:3926759/93‑1 (MQ=255)

ccAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAacat                                                                                         <  1:4095871/93‑1 (MQ=255)

 cAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATg                                                                                        >  1:2361594/1‑93 (MQ=255)

   gTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGaa                                                                                      >  1:1497422/1‑93 (MQ=255)

   gTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAAAATGaa                                                                                      >  1:2121784/1‑93 (MQ=255)

    ttGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGaaa                                                                                     >  1:1189193/1‑93 (MQ=255)

    ttGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGaaa                                                                                     <  1:729310/93‑1 (MQ=255)

      gAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAg                                                                                   >  2:3691928/1‑93 (MQ=255)

      gAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAg                                                                                   <  2:2621014/93‑1 (MQ=255)

       aaCAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGa                                                                                  <  2:5973220/93‑1 (MQ=255)

       aaCAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGa                                                                                  <  2:5902668/93‑1 (MQ=255)

        aCAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGAt                                                                                 >  2:630342/1‑93 (MQ=255)

        aCAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGAt                                                                                 >  1:1633340/1‑93 (MQ=255)

          aaaaaCAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTa                                                                               >  2:1119938/1‑93 (MQ=255)

              aCAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCa                                                                           >  1:2580100/1‑93 (MQ=255)

              aCAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCa                                                                           >  1:6351819/1‑93 (MQ=255)

               cAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCaa                                                                          <  1:5871649/93‑1 (MQ=255)

                aGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTATAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAAt                                                                         >  1:155729/1‑93 (MQ=255)

                aGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAAt                                                                         <  1:2249252/93‑1 (MQ=255)

                aGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAAt                                                                         <  2:1851161/93‑1 (MQ=255)

                aGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAAt                                                                         <  1:4218299/93‑1 (MQ=255)

                aGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAAt                                                                         <  1:1040627/93‑1 (MQ=255)

                 gTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATa                                                                        >  1:2748675/1‑93 (MQ=255)

                   ttACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATaaa                                                                      >  2:5167980/1‑93 (MQ=255)

                    tACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATaaaa                                                                     <  1:4312850/93‑1 (MQ=255)

                    tACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATaaaa                                                                     >  1:6085566/1‑93 (MQ=255)

                     aCATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAAt                                                                    <  2:5841098/93‑1 (MQ=255)

                      cATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATg                                                                   >  1:499962/1‑93 (MQ=255)

                       aTCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGa                                                                  >  1:2925692/1‑93 (MQ=255)

                         ccTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGacc                                                                >  2:1664488/1‑93 (MQ=255)

                          cTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGacca                                                               >  1:1853265/1‑93 (MQ=255)

                           ttGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGaccac                                                              <  1:3415688/93‑1 (MQ=255)

                           ttGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGaccac                                                              <  2:6456965/93‑1 (MQ=255)

                           ttGCTTCTGCGGGAAGGGATTATCAGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGaccac                                                              <  1:5764420/93‑1 (MQ=255)

                            tGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGaacacc                                                             >  1:3922886/1‑93 (MQ=255)

                             gCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCg                                                            >  2:3265651/1‑93 (MQ=255)

                              cTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCga                                                           >  1:4717338/1‑93 (MQ=255)

                              cTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCga                                                           >  2:1322117/1‑93 (MQ=255)

                              cTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCga                                                           <  1:2022605/93‑1 (MQ=255)

                               ttCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCgaa                                                          >  2:230621/1‑93 (MQ=255)

                               ttCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCgaa                                                          >  1:2439955/1‑93 (MQ=255)

                               ttCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCgaa                                                          >  2:5403489/1‑93 (MQ=255)

                                tCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCgaag                                                         >  1:3193000/1‑93 (MQ=255)

                                   gCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAAc                                                      <  1:771654/93‑1 (MQ=255)

                                   gCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAAc                                                      <  2:1231736/93‑1 (MQ=255)

                                      ggAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTgg                                                   <  2:519232/93‑1 (MQ=255)

                                       gAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGc                                                  <  2:1062126/93‑1 (MQ=255)

                                        aaGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCt                                                 <  2:1424207/93‑1 (MQ=255)

                                         aGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTg                                                <  2:4905567/93‑1 (MQ=255)

                                           ggATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGaa                                              >  2:2416423/1‑93 (MQ=255)

                                           ggATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGaa                                              <  1:4633994/93‑1 (MQ=255)

                                           ggATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGaa                                              <  1:90505/93‑1 (MQ=255)

                                           ggATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGaa                                              <  2:469944/93‑1 (MQ=255)

                                            gATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAAt                                             >  2:6049490/1‑93 (MQ=255)

                                             aTTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATg                                            <  1:6057382/93‑1 (MQ=255)

                                              ttATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGc                                           >  1:591572/1‑93 (MQ=255)

                                                aTCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCt                                         >  2:1485056/1‑93 (MQ=255)

                                                aTCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCt                                         <  1:1421062/93‑1 (MQ=255)

                                                aTCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCt                                         >  2:3050222/1‑93 (MQ=255)

                                                 tCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTc                                        <  2:1706503/93‑1 (MQ=255)

                                                   ggATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCgg                                      >  2:5023119/1‑93 (MQ=255)

                                                    gATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGgt                                     >  2:2087477/1‑93 (MQ=255)

                                                     aTTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGgtg                                    <  2:2608655/93‑1 (MQ=255)

                                                     aTTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGgtg                                    >  1:191322/1‑93 (MQ=255)

                                                      ttGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGgtgt                                   <  2:6388308/93‑1 (MQ=255)

                                                           aCAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTa                              >  2:969607/1‑93 (MQ=255)

                                                            cAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaa                             <  1:4201650/93‑1 (MQ=255)

                                                              aCGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaac                           >  1:5237160/1‑93 (MQ=255)

                                                                 cTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaacaaa                        >  2:3156688/1‑93 (MQ=255)

                                                                 cTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaacaaa                        <  1:3023396/93‑1 (MQ=255)

                                                                 cTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaacaaa                        >  1:5453752/1‑93 (MQ=255)

                                                                  ttAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGCCCACCGAAGAGCTGGCTAAATGCCTCGGTGTGGCTaaacaaac                       <  2:5819532/93‑1 (MQ=255)

                                                                  ttAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaacaaac                       <  2:3531105/93‑1 (MQ=255)

                                                                  ttAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaacaaac                       >  1:3303219/1‑93 (MQ=255)

                                                                  ttAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaacaaac                       >  2:4182587/1‑93 (MQ=255)

                                                                  tgggggATTCGCCATAGGAAATAAAAGGAAAAGATTACGCGATAAAATGACCACCGACCCACTTTCTGAATGCCTTGGTGTAACTacaaaaac                       >  2:3434132/4‑93 (MQ=255)

                                                                   tAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACt                      <  1:230996/93‑1 (MQ=255)

                                                                   tAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACt                      >  2:4250645/1‑93 (MQ=255)

                                                                   tAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACt                      <  2:209381/93‑1 (MQ=255)

                                                                    aGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTg                     <  1:1271486/93‑1 (MQ=255)

                                                                       gATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTa                  <  1:1452060/93‑1 (MQ=255)

                                                                        aTTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTaa                 <  2:3757971/93‑1 (MQ=255)

                                                                         ttCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAAc                >  2:4963816/1‑93 (MQ=255)

                                                                              gATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTg           >  2:6197416/1‑93 (MQ=255)

                                                                              gATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTg           <  2:4995864/93‑1 (MQ=255)

                                                                              gATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTg           >  2:5579495/1‑93 (MQ=255)

                                                                              gATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTg           <  1:4905012/93‑1 (MQ=255)

                                                                                tAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGa         >  2:4426241/1‑93 (MQ=255)

                                                                                 aGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGAt        >  1:1931268/1‑93 (MQ=255)

                                                                                  gCAAATAACATGACAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATc       <  1:3691776/93‑1 (MQ=255)

                                                                                  gCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATc       <  2:5069451/93‑1 (MQ=255)

                                                                                  gCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATc       <  2:4053959/93‑1 (MQ=255)

                                                                                    aaaTAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCag     >  1:5219518/1‑93 (MQ=255)

                                                                                    aaaTAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCag     >  2:406353/1‑93 (MQ=255)

                                                                                    aaaTAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCag     >  1:2354679/1‑93 (MQ=255)

                                                                                     aataacATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCaga    >  1:5573322/1‑93 (MQ=255)

                                                                                     aataacATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCaga    >  1:2992912/1‑93 (MQ=255)

                                                                                     aataacATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGCGTGGCTAAAAAAACTGTTAACCGTTGGATCaga    >  2:2887856/1‑93 (MQ=255)

                                                                                      ataacaTGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCagag   <  2:3625401/93‑1 (MQ=255)

                                                                                       taacaTGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAgaga  >  2:1126821/1‑93 (MQ=255)

CCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGA  >  NC_000913/2516742‑2516921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAA  >  NC_000913/2516739‑2516925

ACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAAC                                                                                               <  SRR4302537.6085081/94‑1 (MQ=60)

ACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAAC                                                                                               <  SRR4302537.2490556/94‑1 (MQ=60)

ACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAAC                                                                                               <  SRR4302537.4772434/94‑1 (MQ=60)

 CGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTGGCGGATTGTTACAACGCTTAGGGATTCTCGATAGCAAATAACA                                                                                              >  SRR4302537.287213/1‑94 (MQ=60)

 CGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACA                                                                                              >  SRR4302537.2611568/1‑94 (MQ=60)

 CGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACA                                                                                              <  SRR4302537.1344117/94‑1 (MQ=60)

  GCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACAT                                                                                             <  SRR4302537.2585046/94‑1 (MQ=60)

  GCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACAT                                                                                             <  SRR4302537.4497145/94‑1 (MQ=60)

  GCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACAT                                                                                             >  SRR4302537.3811454/1‑94 (MQ=60)

   CCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATG                                                                                            <  SRR4302537.2374829/94‑1 (MQ=60)

   CCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATG                                                                                            <  SRR4302537.2814882/94‑1 (MQ=60)

   CCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATG                                                                                            >  SRR4302537.2378251/1‑94 (MQ=60)

   CCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATG                                                                                            <  SRR4302537.4124471/94‑1 (MQ=60)

     AGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAA                                                                                          <  SRR4302537.5375632/94‑1 (MQ=60)

     AGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAA                                                                                          >  SRR4302537.4675543/1‑94 (MQ=60)

     AGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAA                                                                                          >  SRR4302537.3397756/1‑94 (MQ=60)

     AGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAA                                                                                          >  SRR4302537.1531944/1‑94 (MQ=60)

     AGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAA                                                                                          >  SRR4302537.1507870/1‑94 (MQ=60)

     AGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAAAATGAA                                                                                          >  SRR4302537.2136715/1‑94 (MQ=60)

      GTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAA                                                                                         >  SRR4302537.1197453/1‑94 (MQ=60)

      GTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAA                                                                                         <  SRR4302537.92231/94‑1 (MQ=60)

      GTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAA                                                                                         >  SRR4302537.4278041/1‑94 (MQ=60)

       TTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAA                                                                                        <  SRR4302537.734291/94‑1 (MQ=60)

        TGAACAAAACCAGTTTACATCCTTGCTTCTGCGGGACGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAG                                                                                       <  SRR4302537.2528137/94‑1 (MQ=60)

        TGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAG                                                                                       <  SRR4302537.883686/94‑1 (MQ=60)

        TGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAG                                                                                       <  SRR4302537.1915491/94‑1 (MQ=60)

         GAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGA                                                                                      >  SRR4302537.4900507/1‑94 (MQ=60)

          AACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGAT                                                                                     <  SRR4302537.4244577/94‑1 (MQ=60)

          AACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGAT                                                                                     >  SRR4302537.1644785/1‑94 (MQ=60)

            CAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTA                                                                                   >  SRR4302537.1434124/1‑94 (MQ=60)

             AAAAACAGTTTACATCCTTGCTTCTGGGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTAC                                                                                  <  SRR4302537.4040408/94‑1 (MQ=60)

               AAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGC                                                                                >  SRR4302537.5710674/1‑94 (MQ=60)

                AACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCA                                                                               >  SRR4302537.2598162/1‑94 (MQ=60)

                AACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCA                                                                               >  SRR4302537.6395184/1‑94 (MQ=60)

                  CAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTATAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAAT                                                                             >  SRR4302537.156837/1‑94 (MQ=60)

                  CAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAAT                                                                             <  SRR4302537.5911926/94‑1 (MQ=60)

                   AGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATA                                                                            >  SRR4302537.2767848/1‑94 (MQ=60)

                   AGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATA                                                                            <  SRR4302537.4247699/94‑1 (MQ=60)

                   AGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATA                                                                            <  SRR4302537.1047815/94‑1 (MQ=60)

                   AGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATA                                                                            <  SRR4302537.2265127/94‑1 (MQ=60)

                   AGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATA                                                                            >  SRR4302537.2182313/1‑94 (MQ=60)

                    GTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAA                                                                           >  SRR4302537.3131801/1‑94 (MQ=60)

                      TTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAA                                                                         >  SRR4302537.6127373/1‑94 (MQ=60)

                       TACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAAT                                                                        <  SRR4302537.4342827/94‑1 (MQ=60)

                       TACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAAT                                                                        <  SRR4302537.5263797/94‑1 (MQ=60)

                        ACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATG                                                                       <  SRR4302537.4942898/94‑1 (MQ=60)

                        ACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATG                                                                       >  SRR4302537.503400/1‑94 (MQ=60)

                        ACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATG                                                                       >  SRR4302537.1440294/1‑94 (MQ=60)

                         CATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGA                                                                      >  SRR4302537.2946239/1‑94 (MQ=60)

                         CATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGA                                                                      <  SRR4302537.3684230/94‑1 (MQ=60)

                           TCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACC                                                                    >  SRR4302537.168856/1‑94 (MQ=60)

                           TCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACC                                                                    >  SRR4302537.1219872/1‑94 (MQ=60)

                            CCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCA                                                                   <  SRR4302537.236609/94‑1 (MQ=60)

                            CCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCA                                                                   <  SRR4302537.4198401/94‑1 (MQ=60)

                            CCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCA                                                                   >  SRR4302537.1866152/1‑94 (MQ=60)

                             CTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCAC                                                                  <  SRR4302537.4360478/94‑1 (MQ=60)

                             CTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCAC                                                                  <  SRR4302537.5055488/94‑1 (MQ=60)

                              TTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACC                                                                 <  SRR4302537.3439873/94‑1 (MQ=60)

                              TTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGAACACC                                                                 >  SRR4302537.3950297/1‑94 (MQ=60)

                              TTGCTTCTGCGGGAAGGGATTATCAGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACC                                                                 <  SRR4302537.5803905/94‑1 (MQ=60)

                                GCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGA                                                               >  SRR4302537.4749961/1‑94 (MQ=60)

                                 CTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAA                                                              >  SRR4302537.3771692/1‑94 (MQ=60)

                                 CTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAA                                                              <  SRR4302537.2036715/94‑1 (MQ=60)

                                 CTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAA                                                              >  SRR4302537.2457136/1‑94 (MQ=60)

                                  TTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAG                                                             <  SRR4302537.1425207/94‑1 (MQ=60)

                                  TTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAG                                                             >  SRR4302537.2130548/1‑94 (MQ=60)

                                  TTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAG                                                             >  SRR4302537.3215451/1‑94 (MQ=60)

                                  TTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAG                                                             <  SRR4302537.760539/94‑1 (MQ=60)

                                    CTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAA                                                           <  SRR4302537.3030082/94‑1 (MQ=60)

                                      GCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACT                                                         <  SRR4302537.776944/94‑1 (MQ=60)

                                         GGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGC                                                      <  SRR4302537.1255985/94‑1 (MQ=60)

                                         GGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGC                                                      >  SRR4302537.3314864/1‑94 (MQ=60)

                                          GAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCT                                                     <  SRR4302537.2431573/94‑1 (MQ=60)

                                           AAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTG                                                    >  SRR4302537.3363881/1‑94 (MQ=60)

                                            AGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGA                                                   >  SRR4302537.3312006/1‑94 (MQ=60)

                                             GGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAA                                                  >  SRR4302537.698049/1‑94 (MQ=60)

                                             GGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAA                                                  >  SRR4302537.565315/1‑94 (MQ=60)

                                              GGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAAT                                                 <  SRR4302537.4666047/94‑1 (MQ=60)

                                              GGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAAT                                                 <  SRR4302537.91167/94‑1 (MQ=60)

                                                ATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGC                                               <  SRR4302537.6098992/94‑1 (MQ=60)

                                                ATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGC                                               >  SRR4302537.595617/1‑94 (MQ=60)

                                                  TATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCT                                             <  SRR4302537.3026759/94‑1 (MQ=60)

                                                   ATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTC                                            <  SRR4302537.1430958/94‑1 (MQ=60)

                                                   ATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTC                                            <  SRR4302537.612262/94‑1 (MQ=60)

                                                      GGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGT                                         >  SRR4302537.5925422/1‑94 (MQ=60)

                                                      GGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGG                                         >  SRR4302537.2211159/1‑94 (MQ=60)

                                                       GATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTG                                        <  SRR4302537.1077197/94‑1 (MQ=60)

                                                       GATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTG                                        >  SRR4302537.192682/1‑94 (MQ=60)

                                                          TGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGG                                     <  SRR4302537.6022096/94‑1 (MQ=60)

                                                          TGTTACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGG                                     >  SRR4302537.1858476/1‑94 (MQ=60)

                                                             TACAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTA                                  >  SRR4302537.588032/1‑94 (MQ=60)

                                                              TCAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAA                                 <  SRR4302537.2381949/94‑1 (MQ=60)

                                                               CAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAA                                <  SRR4302537.4230984/94‑1 (MQ=60)

                                                               CAACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAA                                <  SRR4302537.1542558/94‑1 (MQ=60)

                                                                AACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAAC                               >  SRR4302537.5273194/1‑94 (MQ=60)

                                                                 ACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACA                              <  SRR4302537.746272/94‑1 (MQ=60)

                                                                 ACGCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACA                              >  SRR4302537.2568823/1‑94 (MQ=60)

                                                                   GCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAA                            >  SRR4302537.5490954/1‑94 (MQ=60)

                                                                   GCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAA                            >  SRR4302537.4743339/1‑94 (MQ=60)

                                                                   GCTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAA                            >  SRR4302537.5918050/1‑94 (MQ=60)

                                                                    CTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAAC                           <  SRR4302537.3044470/94‑1 (MQ=60)

                                                                    CTTAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAAC                           >  SRR4302537.3326573/1‑94 (MQ=60)

                                                                      TAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTG                         <  SRR4302537.1488804/94‑1 (MQ=60)

                                                                      TAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTG                         >  SRR4302537.202376/1‑94 (MQ=60)

                                                                      TAGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTG                         <  SRR4302537.232625/94‑1 (MQ=60)

                                                                       AGGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGT                        <  SRR4302537.1280324/94‑1 (MQ=60)

                                                                        GGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTT                       <  SRR4302537.4152413/94‑1 (MQ=60)

                                                                        GGGATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAAGGCCTCGGTGTGGCTAAACAAACTGTT                       <  SRR4302537.6205688/94‑1 (MQ=60)

                                                                          GATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAA                     <  SRR4302537.5438692/94‑1 (MQ=60)

                                                                          GATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAA                     <  SRR4302537.1462178/94‑1 (MQ=60)

                                                                           ATTCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAAC                    <  SRR4302537.1944635/94‑1 (MQ=60)

                                                                             TCGCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCG                  <  SRR4302537.4311887/94‑1 (MQ=60)

                                                                               GCGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTT                >  SRR4302537.6150162/1‑94 (MQ=60)

                                                                                CGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTG               <  SRR4302537.5519013/94‑1 (MQ=60)

                                                                                CGATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTG               >  SRR4302537.1894285/1‑94 (MQ=60)

                                                                                 GATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGG              <  SRR4302537.4938870/94‑1 (MQ=60)

                                                                                 GATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGG              <  SRR4302537.2775111/94‑1 (MQ=60)

                                                                                  ATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGA             >  SRR4302537.2329296/1‑94 (MQ=60)

                                                                                  ATAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGA             >  SRR4302537.2715498/1‑94 (MQ=60)

                                                                                   TAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGAT            <  SRR4302537.4871947/94‑1 (MQ=60)

                                                                                   TAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGAT            <  SRR4302537.2270374/94‑1 (MQ=60)

                                                                                   TAGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGAT            >  SRR4302537.1944702/1‑94 (MQ=60)

                                                                                    AGCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATC           >  SRR4302537.2027634/1‑94 (MQ=60)

                                                                                     GCAAATAACATGACAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCA          <  SRR4302537.3717754/94‑1 (MQ=60)

                                                                                     GCAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCA          <  SRR4302537.5126990/94‑1 (MQ=60)

                                                                                      CAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAG         >  SRR4302537.5255415/1‑94 (MQ=60)

                                                                                      CAAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAG         >  SRR4302537.2371281/1‑94 (MQ=60)

                                                                                       AAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGA        >  SRR4302537.3013795/1‑94 (MQ=60)

                                                                                       AAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGA        >  SRR4302537.1633479/1‑94 (MQ=60)

                                                                                       AAATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGA        >  SRR4302537.5611417/1‑94 (MQ=60)

                                                                                         ATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGA      >  SRR4302537.2967160/1‑94 (MQ=60)

                                                                                         ATAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGA      >  SRR4302537.5959109/1‑94 (MQ=60)

                                                                                          TAACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAA     >  SRR4302537.4121620/1‑94 (MQ=60)

                                                                                           AACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAA    >  SRR4302537.5643873/1‑94 (MQ=60)

                                                                                            ACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAA   >  SRR4302537.4205825/1‑94 (MQ=60)

                                                                                            ACATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAAGGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGCAAA   >  SRR4302537.2715700/1‑94 (MQ=60)

                                                                                             CATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAA  >  SRR4302537.1853799/1‑94 (MQ=60)

                                                                                             CATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAA  <  SRR4302537.25820/94‑1 (MQ=60)

ACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAA  >  NC_000913/2516739‑2516925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`