Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,641,750 A→G L185P (CTG→CCG)  yfgB ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,641,7500AG99.6% 810.4 / ‑5.0 235L185P (CTG→CCG) yfgBhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (120/114);  total (120/115)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 240 total aligned reads displayed.

CACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCC  >  NC_000913/2641661‑2641839
                                                                                         |                                                                                         
atccGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGt                                                                                        <  2:5245098/91‑1 (MQ=255)
  ccGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTg                                                                                      >  1:2293093/1‑93 (MQ=255)
  ccGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTg                                                                                      >  1:3660430/1‑93 (MQ=255)
   cGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCTATCGCCGGAACGACGTTGTTCGGGTTGa                                                                                     >  1:4393605/1‑93 (MQ=255)
    ggAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGag                                                                                    <  1:702602/93‑1 (MQ=255)
    ggAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGag                                                                                    <  2:3203113/93‑1 (MQ=255)
    ggAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGag                                                                                    <  1:3606794/93‑1 (MQ=255)
     gAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGagc                                                                                   >  2:5668473/1‑93 (MQ=255)
     gAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGagc                                                                                   <  1:5666443/93‑1 (MQ=255)
       aGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGagcag                                                                                 <  2:3839606/93‑1 (MQ=255)
         tGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCg                                                                               <  2:1967642/93‑1 (MQ=255)
         tGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCg                                                                               <  1:2631186/93‑1 (MQ=255)
          ggAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCgg                                                                              <  2:3385312/93‑1 (MQ=255)
            aaaGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCt                                                                            >  1:3121235/1‑93 (MQ=255)
             aaGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTc                                                                           >  2:3146807/1‑93 (MQ=255)
                cGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgcc                                                                        <  1:5501972/93‑1 (MQ=255)
                  tGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgccca                                                                      >  2:3467875/1‑93 (MQ=255)
                  tGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgccca                                                                      <  1:256964/93‑1 (MQ=255)
                  tGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgccca                                                                      <  1:5078093/93‑1 (MQ=255)
                  tGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgccca                                                                      <  1:6368097/93‑1 (MQ=255)
                  tGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgccca                                                                      <  2:4628246/93‑1 (MQ=255)
                    aCGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgcccatg                                                                    >  1:2471578/1‑93 (MQ=255)
                     cgcgACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgcccatgc                                                                   >  1:3513662/1‑93 (MQ=255)
                      gcgACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgcccatgcc                                                                  >  1:3155570/1‑93 (MQ=255)
                       cgACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgcccatgccc                                                                 <  2:3954937/93‑1 (MQ=255)
                       cgACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgcccatgccc                                                                 <  1:5118141/93‑1 (MQ=255)
                        gACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgcccatgccca                                                                >  2:2585128/1‑93 (MQ=255)
                         aCGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCgcccatgcccat                                                               <  1:4314330/93‑1 (MQ=255)
                          cGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCcatc                                                              <  1:4917799/93‑1 (MQ=255)
                          cGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCcatc                                                              >  1:957175/1‑93 (MQ=255)
                            tttAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCcatcat                                                            >  1:4076863/1‑93 (MQ=255)
                             ttAGACAGGCCTAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCatcatc                                                           <  2:5247025/93‑1 (MQ=255)
                             ttAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCatcatc                                                           <  2:4543778/93‑1 (MQ=255)
                              tAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCAtcatca                                                          <  1:3335543/93‑1 (MQ=255)
                                 aCAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTa                                                       <  2:3349124/93‑1 (MQ=255)
                                   aGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACg                                                     <  1:464207/93‑1 (MQ=255)
                                    ggCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCCTGCCCATCATCCCTACGt                                                    >  1:5391957/1‑93 (MQ=255)
                                    ggCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGt                                                    >  1:3918699/1‑93 (MQ=255)
                                    ggCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGt                                                    <  2:403532/93‑1 (MQ=255)
                                    ggCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGt                                                    <  2:6413746/93‑1 (MQ=255)
                                      ccAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTa                                                  >  1:1274391/1‑93 (MQ=255)
                                       cAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAg                                                 <  1:5765765/93‑1 (MQ=255)
                                       cAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAg                                                 >  2:1662969/1‑93 (MQ=255)
                                        aaaaCCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAgg                                                >  2:3592008/1‑92 (MQ=255)
                                         aaaCCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTg                                               >  1:6167483/1‑93 (MQ=255)
                                         aaaCCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTg                                               >  1:4422294/1‑93 (MQ=255)
                                            ccGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATc                                            <  2:4155835/93‑1 (MQ=255)
                                             cGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCg                                           >  1:1726543/1‑93 (MQ=255)
                                             cGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCg                                           >  1:3696627/1‑93 (MQ=255)
                                             cGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCg                                           <  2:5785132/93‑1 (MQ=255)
                                              gAAATCATCAAGCATGATTTCCATCGCCGGAATGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACCTTAGTGATCgg                                          >  2:5113335/1‑93 (MQ=255)
                                              gAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCgg                                          >  1:30295/1‑93 (MQ=255)
                                              gAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCgg                                          >  1:624046/1‑93 (MQ=255)
                                               aaaTCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGa                                         <  2:4299795/93‑1 (MQ=255)
                                                  tcatcaAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCAGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGc                                      <  2:4367299/93‑1 (MQ=255)
                                                   catcaAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCt                                     <  2:2504749/93‑1 (MQ=255)
                                                    atcaAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTg                                    >  1:5972600/1‑93 (MQ=255)
                                                     tcaAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCCCGCCCATCATCACGACGTTAGTGATCGGACGCTgc                                   >  2:5010413/1‑92 (MQ=255)
                                                     tcaAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGa                                   >  2:4667276/1‑93 (MQ=255)
                                                      caAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGAc                                  >  2:1139407/1‑93 (MQ=255)
                                                       aaGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGAcc                                 <  1:1446596/93‑1 (MQ=255)
                                                       aaGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGAcc                                 >  1:2929612/1‑93 (MQ=255)
                                                       aaGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGAcc                                 <  2:2988844/93‑1 (MQ=255)
                                                        aGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCg                                >  2:3862832/1‑93 (MQ=255)
                                                        aGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCg                                >  1:17783/1‑93 (MQ=255)
                                                             gATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGAc                           >  1:906588/1‑93 (MQ=255)
                                                             gATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGAc                           <  1:4597592/93‑1 (MQ=255)
                                                              aTTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACt                          <  2:766792/93‑1 (MQ=255)
                                                               tttCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACtt                         <  1:861379/93‑1 (MQ=255)
                                                               tttCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACtt                         >  1:2180701/1‑93 (MQ=255)
                                                                ttCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACttt                        <  2:4636924/93‑1 (MQ=255)
                                                                 tCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACtttt                       >  2:5951794/1‑93 (MQ=255)
                                                                 tCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACtttt                       >  1:4896813/1‑93 (MQ=255)
                                                                  ccATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTtg                      >  1:1644268/1‑93 (MQ=255)
                                                                   cATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTtgc                     <  1:2947325/93‑1 (MQ=255)
                                                                    aTCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTtgct                    <  2:2068443/93‑1 (MQ=255)
                                                                    aTCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTtgct                    <  2:1523278/93‑1 (MQ=255)
                                                                     tCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTtgctg                   <  1:5945241/93‑1 (MQ=255)
                                                                      cGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTtgctgc                  <  1:3274020/93‑1 (MQ=255)
                                                                       gCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTgcg                 <  1:2419056/93‑1 (MQ=255)
                                                                       ccctgAACGCGGTTGTTCGGGTTGAGCAGCGGCTCTCCCATGCCCCTCATGACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTgcg                 <  1:4130835/89‑1 (MQ=255)
                                                                           gAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCcgc             >  2:4721976/1‑92 (MQ=255)
                                                                           gAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCcga             <  1:4946695/93‑1 (MQ=255)
                                                                           gAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCcga             <  2:2784064/93‑1 (MQ=255)
                                                                           gAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCcga             <  2:120996/93‑1 (MQ=255)
                                                                            aaCGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCcgac            <  2:4274668/93‑1 (MQ=255)
                                                                             acgacgTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCcgacg           <  1:1261560/93‑1 (MQ=255)
                                                                             acgacgTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCcgacg           >  2:2303168/1‑93 (MQ=255)
                                                                             acgacgTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCcgacg           >  2:5382004/1‑93 (MQ=255)
                                                                                acgTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGAtt        >  2:5835257/1‑93 (MQ=255)
                                                                                acgTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGAtt        >  2:1957721/1‑93 (MQ=255)
                                                                                 cgTTGTTCGGGTTGAGCAGCGGCTTGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGGTGCGCCGACGAttt       >  2:914474/1‑93 (MQ=255)
                                                                                 cgTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGAttt       >  1:4158098/1‑93 (MQ=255)
                                                                                 cgTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGAttt       <  1:3994568/93‑1 (MQ=255)
                                                                                     gttCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgc   >  1:5893052/1‑93 (MQ=255)
                                                                                     gttCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgc   <  2:271722/93‑1 (MQ=255)
                                                                                     gttCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgc   >  1:5331215/1‑93 (MQ=255)
                                                                                      ttCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgcc  >  2:1248562/1‑93 (MQ=255)
                                                                                      ttCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgcc  <  1:1357735/93‑1 (MQ=255)
                                                                                      ttCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgcc  <  1:3936358/93‑1 (MQ=255)
                                                                                         |                                                                                         
CACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCC  >  NC_000913/2641661‑2641839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCAC  >  NC_000913/2641661‑2641843
                                                                                         |                                                                                             
CACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTT                                                                                           >  SRR4302537.4240310/1‑94 (MQ=60)
 ACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTG                                                                                          >  SRR4302537.2309279/1‑94 (MQ=60)
 ACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTG                                                                                          >  SRR4302537.4946747/1‑94 (MQ=60)
 ACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTG                                                                                          >  SRR4302537.3686195/1‑94 (MQ=60)
  CCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCTATCGCCGGAACGACGTTGTTCGGGTTGA                                                                                         >  SRR4302537.4423917/1‑94 (MQ=60)
    GGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGC                                                                                       <  SRR4302537.707412/94‑1 (MQ=60)
    GGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGC                                                                                       <  SRR4302537.3632217/94‑1 (MQ=60)
     GAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCA                                                                                      <  SRR4302537.5705211/94‑1 (MQ=60)
     GAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCA                                                                                      <  SRR4302537.6274222/94‑1 (MQ=60)
       AGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGC                                                                                    <  SRR4302537.2100740/94‑1 (MQ=60)
         TGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGG                                                                                  <  SRR4302537.2649609/94‑1 (MQ=60)
          GGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGC                                                                                 >  SRR4302537.221009/1‑94 (MQ=60)
           GAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCT                                                                                >  SRR4302537.3143063/1‑94 (MQ=60)
           GAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCT                                                                                <  SRR4302537.2441882/94‑1 (MQ=60)
            AAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTC                                                                               <  SRR4302537.4093703/94‑1 (MQ=60)
               GCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCC                                                                            <  SRR4302537.1489950/94‑1 (MQ=60)
                CGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCC                                                                           <  SRR4302537.5539541/94‑1 (MQ=60)
                  tgcaGCGACGTTTAGACAGGCCAAACCCGAAATCATCAAGCATGATGTTCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCAT                                                                         <  SRR4302537.3072000/90‑1 (MQ=60)
                  TGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCAT                                                                         <  SRR4302537.258785/94‑1 (MQ=60)
                  TGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCAT                                                                         <  SRR4302537.5113051/94‑1 (MQ=60)
                  TGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCAT                                                                         <  SRR4302537.6411528/94‑1 (MQ=60)
                   GACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATG                                                                        >  SRR4302537.2488952/1‑94 (MQ=60)
                   GACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATC                                                                        >  SRR4302537.1700548/1‑94 (MQ=60)
                    ACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGC                                                                       <  SRR4302537.5516060/94‑1 (MQ=60)
                    ACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGC                                                                       <  SRR4302537.3285155/94‑1 (MQ=60)
                    ACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGC                                                                       >  SRR4302537.3538519/1‑94 (MQ=60)
                     CGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCC                                                                      <  SRR4302537.4374860/94‑1 (MQ=60)
                     CGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCC                                                                      >  SRR4302537.3177722/1‑94 (MQ=60)
                       CGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCA                                                                    <  SRR4302537.5153412/94‑1 (MQ=60)
                        GACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCGATGTCCAT                                                                   >  SRR4302537.5676679/1‑94 (MQ=60)
                        GACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGGTGTTCGGGTTGAGCAGCGGCTCGCGCATGGCCAT                                                                   >  SRR4302537.6006662/1‑94 (MQ=60)
                         ACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATC                                                                  >  SRR4302537.963775/1‑94 (MQ=60)
                         ACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATC                                                                  <  SRR4302537.4344318/94‑1 (MQ=60)
                          CGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAGCGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCA                                                                 <  SRR4302537.3223942/94‑1 (MQ=60)
                          CGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCA                                                                 <  SRR4302537.4951726/94‑1 (MQ=60)
                           GTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGCCGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCAT                                                                <  SRR4302537.4697795/94‑1 (MQ=60)
                           GTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCAT                                                                >  SRR4302537.4105331/1‑94 (MQ=60)
                           GTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCAT                                                                <  SRR4302537.3761703/94‑1 (MQ=60)
                            TTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATC                                                               <  SRR4302537.5142288/94‑1 (MQ=60)
                             TTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCA                                                              >  SRR4302537.1695462/1‑94 (MQ=60)
                              TAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCAC                                                             <  SRR4302537.3359149/94‑1 (MQ=60)
                                GACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTA                                                           >  SRR4302537.4003597/1‑94 (MQ=60)
                                  CAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACG                                                         <  SRR4302537.3952990/94‑1 (MQ=60)
                                   AGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCCTGCCCATCATCCCTACGT                                                        >  SRR4302537.5428787/1‑94 (MQ=60)
                                   AGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGT                                                        >  SRR4302537.5821088/1‑94 (MQ=60)
                                   AGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGT                                                        >  SRR4302537.3946091/1‑94 (MQ=60)
                                   AGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGT                                                        <  SRR4302537.467404/94‑1 (MQ=60)
                                    GGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTT                                                       <  SRR4302537.4152693/94‑1 (MQ=60)
                                    GGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTT                                                       >  SRR4302537.3720855/1‑94 (MQ=60)
                                     GCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTA                                                      >  SRR4302537.1283253/1‑94 (MQ=60)
                                      CCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAG                                                     >  SRR4302537.617966/1‑94 (MQ=60)
                                       CAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGT                                                    <  SRR4302537.5805259/94‑1 (MQ=60)
                                        AAAACCGAAATCATCAAGCATGCTTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGGGGCTCGGCCATGCCCATCATCACTACGTTAGTG                                                   >  SRR4302537.6209947/1‑94 (MQ=60)
                                        AAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTG                                                   >  SRR4302537.4452836/1‑94 (MQ=60)
                                        AAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTG                                                   >  SRR4302537.717391/1‑94 (MQ=60)
                                        AAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTG                                                   >  SRR4302537.6209948/1‑94 (MQ=60)
                                         AAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGA                                                  >  SRR4302537.3580117/1‑94 (MQ=60)
                                         AAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGA                                                  <  SRR4302537.598102/94‑1 (MQ=60)
                                            CCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTTCGTTAGTGGTCG                                               >  SRR4302537.2956764/1‑94 (MQ=60)
                                            CCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCG                                               <  SRR4302537.1304155/94‑1 (MQ=60)
                                            CCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCG                                               >  SRR4302537.3722640/1‑94 (MQ=60)
                                            CCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCG                                               >  SRR4302537.5931817/1‑94 (MQ=60)
                                            CCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCG                                               >  SRR4302537.1738606/1‑94 (MQ=60)
                                             CGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGG                                              >  SRR4302537.628303/1‑94 (MQ=60)
                                             CGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGG                                              >  SRR4302537.30538/1‑94 (MQ=60)
                                             CGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGG                                              <  SRR4302537.2788104/94‑1 (MQ=60)
                                               AAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGAC                                            <  SRR4302537.2666965/94‑1 (MQ=60)
                                               AAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGAC                                            <  SRR4302537.6336766/94‑1 (MQ=60)
                                                AATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACG                                           >  SRR4302537.4609255/1‑94 (MQ=60)
                                                AATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACG                                           >  SRR4302537.341362/1‑94 (MQ=60)
                                                AATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACG                                           >  SRR4302537.6326230/1‑94 (MQ=60)
                                                AATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACG                                           <  SRR4302537.905995/94‑1 (MQ=60)
                                                AATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACG                                           >  SRR4302537.3190853/1‑94 (MQ=60)
                                                 ATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGC                                          >  SRR4302537.4316057/1‑94 (MQ=60)
                                                 ATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGC                                          >  SRR4302537.3037011/1‑94 (MQ=60)
                                                 ATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGC                                          >  SRR4302537.4541009/1‑94 (MQ=60)
                                                  TCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCT                                         <  SRR4302537.1276499/94‑1 (MQ=60)
                                                   CATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTG                                        <  SRR4302537.537880/94‑1 (MQ=60)
                                                   CATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTG                                        >  SRR4302537.6013594/1‑94 (MQ=60)
                                                    ATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGCTCGGACGCCGA                                       >  SRR4302537.5306397/1‑94 (MQ=60)
                                                    ATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGA                                       >  SRR4302537.1771331/1‑94 (MQ=60)
                                                    ATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGA                                       <  SRR4302537.5748705/94‑1 (MQ=60)
                                                     TCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGAC                                      <  SRR4302537.410678/94‑1 (MQ=60)
                                                      CAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACC                                     <  SRR4302537.3170166/94‑1 (MQ=60)
                                                      CAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACC                                     >  SRR4302537.2950185/1‑94 (MQ=60)
                                                      CAAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACC                                     <  SRR4302537.2290587/94‑1 (MQ=60)
                                                       AAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG                                    >  SRR4302537.17924/1‑94 (MQ=60)
                                                       AAGCATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG                                    <  SRR4302537.1456676/94‑1 (MQ=60)
                                                          CATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTG                                 >  SRR4302537.1419798/1‑94 (MQ=60)
                                                           ATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGA                                >  SRR4302537.506927/1‑94 (MQ=60)
                                                           ATGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGA                                <  SRR4302537.907533/94‑1 (MQ=60)
                                                            TGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGAC                               <  SRR4302537.5290314/94‑1 (MQ=60)
                                                            TGATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGAC                               >  SRR4302537.912851/1‑94 (MQ=60)
                                                             GATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACT                              <  SRR4302537.4629410/94‑1 (MQ=60)
                                                              ATTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTT                             >  SRR4302537.2196066/1‑94 (MQ=60)
                                                               TTTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTT                            <  SRR4302537.867283/94‑1 (MQ=60)
                                                                TTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCTGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTT                           <  SRR4302537.665327/94‑1 (MQ=60)
                                                                TTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTT                           >  SRR4302537.4930618/1‑94 (MQ=60)
                                                                TTCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTT                           >  SRR4302537.480925/1‑94 (MQ=60)
                                                                 TCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTG                          <  SRR4302537.3701660/94‑1 (MQ=60)
                                                                 TCCATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTG                          >  SRR4302537.1655789/1‑94 (MQ=60)
                                                                   CATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCT                        <  SRR4302537.2968014/94‑1 (MQ=60)
                                                                   CATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGACCATCATCACTACGTTAGTGATAGGACGCAGACCGGGGACTTTTGCT                        >  SRR4302537.1141422/1‑94 (MQ=60)
                                                                    ATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTGTGCTG                       >  SRR4302537.2442488/1‑94 (MQ=60)
                                                                    ATCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGCGACTTTTGCTG                       >  SRR4302537.930578/1‑94 (MQ=60)
                                                                     TCGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGC                      <  SRR4302537.5986048/94‑1 (MQ=60)
                                                                      CGCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCG                     <  SRR4302537.3297125/94‑1 (MQ=60)
                                                                       ccctgaacgcgGTTGTTCGGGTTGAGCAGCGGCTCTCCCATGCCCCTCATGACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGC                    <  SRR4302537.4159663/83‑1 (MQ=60)
                                                                       GCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGC                    <  SRR4302537.2436106/94‑1 (MQ=60)
                                                                       GCCGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGC                    <  SRR4302537.5031595/94‑1 (MQ=60)
                                                                         CGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCG                  >  SRR4302537.2648128/1‑94 (MQ=60)
                                                                         CGGAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCG                  <  SRR4302537.5542021/94‑1 (MQ=60)
                                                                           GAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGAC                <  SRR4302537.1376354/94‑1 (MQ=60)
                                                                           GAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGAC                <  SRR4302537.4439190/94‑1 (MQ=60)
                                                                           GAACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGAC                <  SRR4302537.4980800/94‑1 (MQ=60)
                                                                            AACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACG               >  SRR4302537.2390691/1‑94 (MQ=60)
                                                                             ACGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGA              <  SRR4302537.1270334/94‑1 (MQ=60)
                                                                              CGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGAT             >  SRR4302537.4974541/1‑94 (MQ=60)
                                                                              CGACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGAT             >  SRR4302537.3493481/1‑94 (MQ=60)
                                                                               GACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATT            >  SRR4302537.2534982/1‑94 (MQ=60)
                                                                               GACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATT            >  SRR4302537.3654118/1‑94 (MQ=60)
                                                                                ACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTT           >  SRR4302537.4187137/1‑94 (MQ=60)
                                                                                ACGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTT           >  SRR4302537.3308314/1‑94 (MQ=60)
                                                                                 CGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTT          >  SRR4302537.4633572/1‑94 (MQ=60)
                                                                                 CGTTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTT          <  SRR4302537.4022532/94‑1 (MQ=60)
                                                                                 CGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTT          <  SRR4302537.3098876/94‑1 (MQ=60)
                                                                                   GTGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCG        <  SRR4302537.3003528/94‑1 (MQ=60)
                                                                                    TGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGC       >  SRR4302537.5367786/1‑94 (MQ=60)
                                                                                    TGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGC       >  SRR4302537.4160079/1‑94 (MQ=60)
                                                                                    TGTTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGC       >  SRR4302537.5933486/1‑94 (MQ=60)
                                                                                      TTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCG     <  SRR4302537.1367189/94‑1 (MQ=60)
                                                                                      TTCGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCG     <  SRR4302537.3963870/94‑1 (MQ=60)
                                                                                      TTCGGGTGGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCG     <  SRR4302537.3963869/94‑1 (MQ=60)
                                                                                        CGGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCA   >  SRR4302537.2577359/1‑94 (MQ=60)
                                                                                         GGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCAC  >  SRR4302537.3691940/1‑94 (MQ=60)
                                                                                         |                                                                                             
CACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCAC  >  NC_000913/2641661‑2641843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑