Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,668,735 T→C I109T (ATC→ACC)  hcaA2 → small terminal subunit of phenylpropionate dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,668,7350TC95.0% 558.7 / 14.2 180I109T (ATC→ACC) hcaA2small terminal subunit of phenylpropionate dioxygenase
Reads supporting (aligned to +/- strand):  ref base T (2/7);  new base C (69/102);  total (71/109)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 190 total aligned reads displayed.

CCGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTC  >  NC_000913/2668646‑2668823
                                                                                         |                                                                                        
ccGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACAcccc                                                                                       >  2:1726578/1‑93 (MQ=255)
ccGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACAcccc                                                                                       >  1:855141/1‑93 (MQ=255)
ccGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACAcccc                                                                                       >  1:2187545/1‑93 (MQ=255)
   tCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGATACCCCaaa                                                                                    <  2:6330507/93‑1 (MQ=255)
   tCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCaaa                                                                                    <  1:6086656/93‑1 (MQ=255)
   tCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGCCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCaaa                                                                                    >  1:612354/1‑93 (MQ=255)
    cTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAAc                                                                                   >  2:1793407/1‑93 (MQ=255)
     tGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACg                                                                                  >  2:1817352/1‑93 (MQ=255)
     tGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACg                                                                                  >  1:4618428/1‑93 (MQ=255)
      ggAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGt                                                                                 <  2:5331409/93‑1 (MQ=255)
       gAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTa                                                                                <  1:3194927/93‑1 (MQ=255)
        aaaCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTAt                                                                               <  1:4938532/93‑1 (MQ=255)
         aaCCGGCATTGCCTGGGGAGAAGAGGCGGCCTTACGCCCCCGGTAATTAATCAGCAACTGCCAAAAAAGCGAAACCGACACCCCAAACGTAtt                                                                              <  2:991474/93‑1 (MQ=255)
          aCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTAttt                                                                             >  1:3523844/1‑93 (MQ=255)
             ggCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCt                                                                          >  1:2354171/1‑93 (MQ=255)
             ggCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCt                                                                          >  1:5303194/1‑93 (MQ=255)
             ggCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCt                                                                          <  1:3006799/93‑1 (MQ=255)
              gCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCtg                                                                         <  1:5318493/93‑1 (MQ=255)
               cATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCtgt                                                                        <  2:2557904/93‑1 (MQ=255)
               cATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCtgt                                                                        <  2:3667552/93‑1 (MQ=255)
                aTGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCtgtg                                                                       <  2:1937149/93‑1 (MQ=255)
                 tGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGc                                                                      <  2:5544887/93‑1 (MQ=255)
                  gcccTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAACCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCg                                                                     <  2:4539933/91‑1 (MQ=255)
                       gggCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTaa                                                                >  1:5120122/1‑93 (MQ=255)
                        ggCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTaaa                                                               <  2:4508137/93‑1 (MQ=255)
                         gCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAAt                                                              <  1:3609864/93‑1 (MQ=255)
                         gCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAAt                                                              <  1:6360284/93‑1 (MQ=255)
                         gCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGACAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGCGCGGGGAAAt                                                              >  2:2149805/1‑93 (MQ=255)
                          cAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAAtt                                                             <  1:28579/93‑1 (MQ=255)
                           agaagaGCCGCCGTCACGCCCCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTa                                                            <  1:5037527/93‑1 (MQ=255)
                           agaagaGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTa                                                            >  1:113151/1‑93 (MQ=255)
                            gaagaGCCGCCGTCACGCATCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGCATTTGCTGTGCGGGTAAATTAt                                                           >  1:390854/1‑93 (MQ=255)
                            gaagaGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTAt                                                           >  1:3910948/1‑93 (MQ=255)
                             aagaGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATc                                                          >  1:3668667/1‑93 (MQ=255)
                             aagaGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATc                                                          <  2:4900226/93‑1 (MQ=255)
                             aagaGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATc                                                          <  1:1927084/93‑1 (MQ=255)
                              agagCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCt                                                         <  1:3729389/93‑1 (MQ=255)
                              agagCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCt                                                         <  2:3375417/93‑1 (MQ=255)
                              agagCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCt                                                         <  2:1627218/93‑1 (MQ=255)
                                     ccgTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTAt                                                  >  2:5886498/1‑93 (MQ=255)
                                      cgTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATc                                                 <  2:3920976/93‑1 (MQ=255)
                                       gTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCg                                                <  1:1430121/93‑1 (MQ=255)
                                       gTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCg                                                <  2:2862913/93‑1 (MQ=255)
                                       gTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCg                                                >  2:4745550/1‑93 (MQ=255)
                                       gTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCg                                                >  1:5939790/1‑93 (MQ=255)
                                       gTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCg                                                >  2:5053819/1‑93 (MQ=255)
                                       gTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCg                                                <  2:4898065/93‑1 (MQ=255)
                                        tCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCgg                                               >  2:4790433/1‑93 (MQ=255)
                                        tCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCgg                                               <  2:3233751/93‑1 (MQ=255)
                                        tCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCgg                                               >  1:5243971/1‑93 (MQ=255)
                                             cACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGcaca                                          >  1:2884358/1‑93 (MQ=255)
                                             cACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGcaca                                          >  2:2831409/1‑93 (MQ=255)
                                              aCCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACaa                                         <  1:2709929/93‑1 (MQ=255)
                                              aCCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACaa                                         >  1:3617199/1‑93 (MQ=255)
                                               cccGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACaaa                                        >  2:5994667/1‑93 (MQ=255)
                                                 cGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACaaaaa                                      >  2:4327123/1‑93 (MQ=255)
                                                 cGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACaaaaa                                      >  1:811967/1‑93 (MQ=255)
                                                 cGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACaaaaa                                      <  2:1364708/93‑1 (MQ=255)
                                                  gTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAag                                     <  1:3031380/93‑1 (MQ=255)
                                                  gTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAag                                     >  1:1978599/1‑93 (MQ=255)
                                                   tCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAaga                                    >  2:5432432/1‑93 (MQ=255)
                                                   tCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAaga                                    >  2:626820/1‑93 (MQ=255)
                                                   tCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAaga                                    >  2:2483823/1‑93 (MQ=255)
                                                    cACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAagag                                   <  1:6144639/93‑1 (MQ=255)
                                                    cACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAagag                                   <  2:693934/93‑1 (MQ=255)
                                                      cTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAgcg                                 >  1:1035317/1‑93 (MQ=255)
                                                      cTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAgcg                                 >  1:3189120/1‑93 (MQ=255)
                                                       ttAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAgcgc                                >  1:5890305/1‑93 (MQ=255)
                                                       ttAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAgcgc                                >  2:1229698/1‑93 (MQ=255)
                                                          aTCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGAt                             >  1:3103820/1‑93 (MQ=255)
                                                           tCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATg                            >  1:1743287/1‑93 (MQ=255)
                                                            cAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGa                           <  1:4737704/93‑1 (MQ=255)
                                                              gCAACTACCAGATAAGCGAAACCGACACCACAAACGTATTTGCTGAGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGaaa                         <  2:4607029/93‑1 (MQ=255)
                                                               cAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAAc                        <  2:5605324/93‑1 (MQ=255)
                                                                aaCTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACa                       >  2:4661596/1‑93 (MQ=255)
                                                                      cAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTAt                 >  1:1230572/1‑93 (MQ=255)
                                                                      cAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTAt                 >  1:3324932/1‑93 (MQ=255)
                                                                       aGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATg                >  2:4738899/1‑93 (MQ=255)
                                                                       aGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATg                >  1:1596438/1‑93 (MQ=255)
                                                                       aGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATg                <  1:1669814/93‑1 (MQ=255)
                                                                        gATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGt               <  2:606308/93‑1 (MQ=255)
                                                                          tAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTg             <  1:5791662/93‑1 (MQ=255)
                                                                          tAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTg             >  2:855916/1‑93 (MQ=255)
                                                                           aaGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTATATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTgg            <  1:4386209/93‑1 (MQ=255)
                                                                            aGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGa           <  1:1282971/93‑1 (MQ=255)
                                                                             gCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGaa          >  1:5759325/1‑93 (MQ=255)
                                                                             gCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGaa          <  1:245843/93‑1 (MQ=255)
                                                                              cGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAAc         <  2:1888555/93‑1 (MQ=255)
                                                                               gAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAAcg        >  1:1972656/1‑93 (MQ=255)
                                                                               gAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAAcg        <  1:2931088/93‑1 (MQ=255)
                                                                                 aaCCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAAcgcg      >  1:6012754/1‑93 (MQ=255)
                                                                                 aaCCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAAcgcg      <  1:5644789/93‑1 (MQ=255)
                                                                                  aCCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGt     >  2:6426222/1‑93 (MQ=255)
                                                                                    cGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGttt   <  2:6369664/93‑1 (MQ=255)
                                                                                    cGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGttt   <  2:1162814/93‑1 (MQ=255)
                                                                                     gACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTc  <  2:3228547/93‑1 (MQ=255)
                                                                                     gACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTc  <  1:886963/93‑1 (MQ=255)
                                                                                     gACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTc  <  1:5439793/93‑1 (MQ=255)
                                                                                     gACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTc  <  1:485537/93‑1 (MQ=255)
                                                                                     gACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTc  <  1:1009862/93‑1 (MQ=255)
                                                                                         |                                                                                        
CCGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTC  >  NC_000913/2668646‑2668823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAA  >  NC_000913/2668645‑2668828
                                                                                          |                                                                                             
CCCGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCC                                                                                            >  SRR4302537.861011/1‑94 (MQ=60)
CCCGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCC                                                                                            >  SRR4302537.5252300/1‑94 (MQ=60)
CCCGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCC                                                                                            >  SRR4302537.2202956/1‑94 (MQ=60)
 CCGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCA                                                                                           <  SRR4302537.6150223/94‑1 (MQ=60)
   GTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAA                                                                                         >  SRR4302537.3711696/1‑94 (MQ=60)
   GTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAA                                                                                         >  SRR4302537.1301157/1‑94 (MQ=60)
   GTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGCCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAA                                                                                         >  SRR4302537.616533/1‑94 (MQ=60)
    TCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAAC                                                                                        <  SRR4302537.6128468/94‑1 (MQ=60)
     CTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACG                                                                                       >  SRR4302537.4650414/1‑94 (MQ=60)
       GGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTA                                                                                     >  SRR4302537.5283407/1‑94 (MQ=60)
       GGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTA                                                                                     >  SRR4302537.3038524/1‑94 (MQ=60)
       GGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTA                                                                                     >  SRR4302537.4802919/1‑94 (MQ=60)
        GAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTAT                                                                                    <  SRR4302537.3217391/94‑1 (MQ=60)
        GAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTAT                                                                                    >  SRR4302537.6468971/1‑94 (MQ=60)
         AAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATT                                                                                   <  SRR4302537.4972595/94‑1 (MQ=60)
          AACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTT                                                                                  >  SRR4302537.3548773/1‑94 (MQ=60)
             GGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCT                                                                               >  SRR4302537.2370771/1‑94 (MQ=60)
             GGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCT                                                                               >  SRR4302537.5339649/1‑94 (MQ=60)
              GGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTG                                                                              <  SRR4302537.3027736/94‑1 (MQ=60)
               GCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGT                                                                             <  SRR4302537.5355006/94‑1 (MQ=60)
                     CCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGT                                                                       <  SRR4302537.3274459/94‑1 (MQ=60)
                     CCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGT                                                                       >  SRR4302537.5827639/1‑94 (MQ=60)
                      CTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTA                                                                      <  SRR4302537.2552662/94‑1 (MQ=60)
                       TGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAA                                                                     >  SRR4302537.5155410/1‑94 (MQ=60)
                         GGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAAT                                                                   <  SRR4302537.5082309/94‑1 (MQ=60)
                          GCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATT                                                                  <  SRR4302537.6403680/94‑1 (MQ=60)
                          GCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATT                                                                  <  SRR4302537.3635310/94‑1 (MQ=60)
                           CAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTA                                                                 <  SRR4302537.28808/94‑1 (MQ=60)
                           CAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTA                                                                 >  SRR4302537.113975/1‑94 (MQ=60)
                            AGAAGAGCCGCCGTCACGCCCCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTAT                                                                <  SRR4302537.5072206/94‑1 (MQ=60)
                            AGAAGAGCCGCCGTCACGCATCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGCATTTGCTGTGCGGGTAAATTAT                                                                >  SRR4302537.393556/1‑94 (MQ=60)
                            AGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTAT                                                                <  SRR4302537.2987924/94‑1 (MQ=60)
                            AGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTAT                                                                >  SRR4302537.3938285/1‑94 (MQ=60)
                             GAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATC                                                               >  SRR4302537.3694481/1‑94 (MQ=60)
                              AAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCT                                                              >  SRR4302537.2393495/1‑94 (MQ=60)
                              AAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCT                                                              <  SRR4302537.1940458/94‑1 (MQ=60)
                               AGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTG                                                             <  SRR4302537.3755586/94‑1 (MQ=60)
                                    CGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTA                                                        <  SRR4302537.1220346/94‑1 (MQ=60)
                                     GCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTAT                                                       <  SRR4302537.3656505/94‑1 (MQ=60)
                                      CCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATC                                                      >  SRR4302537.3588447/1‑94 (MQ=60)
                                       CGTCACGCACCCGTCACTTAATCAGCAACTGTCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCG                                                     <  SRR4302537.3163136/94‑1 (MQ=60)
                                       CGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCG                                                     >  SRR4302537.5980557/1‑94 (MQ=60)
                                       CGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCG                                                     <  SRR4302537.6078866/94‑1 (MQ=60)
                                        GTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGG                                                    >  SRR4302537.5280055/1‑94 (MQ=60)
                                        GTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGG                                                    <  SRR4302537.1440077/94‑1 (MQ=60)
                                           TCGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCA                                                 <  SRR4302537.1461626/94‑1 (MQ=60)
                                           ACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCA                                                 <  SRR4302537.6027706/94‑1 (MQ=60)
                                             GCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACA                                               >  SRR4302537.2904599/1‑94 (MQ=60)
                                             GCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAAACGACACCCCACACGTCTTTTCTGTGCGGGTAAATTATCTcatttctcgggcgta                                               >  SRR4302537.2904598/1‑79 (MQ=60)
                                              CACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAA                                              >  SRR4302537.1955163/1‑94 (MQ=60)
                                              CACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAA                                              >  SRR4302537.3642704/1‑94 (MQ=60)
                                              CACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAA                                              <  SRR4302537.836172/94‑1 (MQ=60)
                                               ACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAA                                             <  SRR4302537.2728854/94‑1 (MQ=60)
                                                 CCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAA                                           >  SRR4302537.817532/1‑94 (MQ=60)
                                                  CGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAG                                          >  SRR4302537.1992347/1‑94 (MQ=60)
                                                   GTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGA                                         <  SRR4302537.3052515/94‑1 (MQ=60)
                                                   GTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTACTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGA                                         <  SRR4302537.3487479/94‑1 (MQ=60)
                                                    TCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAG                                        >  SRR4302537.3639900/1‑94 (MQ=60)
                                                    TCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAG                                        <  SRR4302537.5940186/94‑1 (MQ=60)
                                                    TCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAG                                        <  SRR4302537.2923959/94‑1 (MQ=60)
                                                     CACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGC                                       <  SRR4302537.6186894/94‑1 (MQ=60)
                                                      ACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCG                                      >  SRR4302537.1042448/1‑94 (MQ=60)
                                                      ACTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCG                                      >  SRR4302537.3211550/1‑94 (MQ=60)
                                                       CTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGC                                     >  SRR4302537.5930722/1‑94 (MQ=60)
                                                       CTTAATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAACGC                                     <  SRR4302537.4554368/94‑1 (MQ=60)
                                                          AATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGAT                                  <  SRR4302537.4735958/94‑1 (MQ=60)
                                                          AATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGAT                                  >  SRR4302537.3125500/1‑94 (MQ=60)
                                                           ATCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATG                                 >  SRR4302537.1755439/1‑94 (MQ=60)
                                                            TCAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGA                                <  SRR4302537.1885698/94‑1 (MQ=60)
                                                             CAGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAA                               <  SRR4302537.4770452/94‑1 (MQ=60)
                                                              AGCAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAA                              >  SRR4302537.1065379/1‑94 (MQ=60)
                                                                CAACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACA                            >  SRR4302537.636901/1‑94 (MQ=60)
                                                                  ACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATT                          <  SRR4302537.2147762/94‑1 (MQ=60)
                                                                  ACTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATT                          >  SRR4302537.2864887/1‑94 (MQ=60)
                                                                   CTGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTC                         >  SRR4302537.4163659/1‑94 (MQ=60)
                                                                    TGCCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCT                        <  SRR4302537.2248309/94‑1 (MQ=60)
                                                                      CCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTAT                      >  SRR4302537.3830085/1‑94 (MQ=60)
                                                                      CCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTAT                      >  SRR4302537.3348458/1‑94 (MQ=60)
                                                                      CCAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTAT                      >  SRR4302537.1239129/1‑94 (MQ=60)
                                                                       CAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATG                     >  SRR4302537.1607592/1‑94 (MQ=60)
                                                                       CAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATG                     <  SRR4302537.1732337/94‑1 (MQ=60)
                                                                       CAGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATG                     <  SRR4302537.1677331/94‑1 (MQ=60)
                                                                        AGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGT                    <  SRR4302537.5491140/94‑1 (MQ=60)
                                                                        AGATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGT                    <  SRR4302537.1681509/94‑1 (MQ=60)
                                                                          ATAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTG                  <  SRR4302537.1248825/94‑1 (MQ=60)
                                                                           TAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGG                 <  SRR4302537.5831371/94‑1 (MQ=60)
                                                                           TAAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGG                 >  SRR4302537.5909182/1‑94 (MQ=60)
                                                                            AAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTATATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGA                <  SRR4302537.4416472/94‑1 (MQ=60)
                                                                            AAGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGA                >  SRR4302537.3214241/1‑94 (MQ=60)
                                                                             AGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAA               <  SRR4302537.1291890/94‑1 (MQ=60)
                                                                             AGCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAA               >  SRR4302537.5798775/1‑94 (MQ=60)
                                                                              GCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAAC              <  SRR4302537.247585/94‑1 (MQ=60)
                                                                              GCGAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAAC              <  SRR4302537.5034956/94‑1 (MQ=60)
                                                                               CGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACG             >  SRR4302537.1986370/1‑94 (MQ=60)
                                                                                GAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGC            <  SRR4302537.2951670/94‑1 (MQ=60)
                                                                                GAAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGC            <  SRR4302537.5700928/94‑1 (MQ=60)
                                                                                 AAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCG           >  SRR4302537.6054065/1‑94 (MQ=60)
                                                                                 AAACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCG           <  SRR4302537.4952286/94‑1 (MQ=60)
                                                                                  AACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGT          >  SRR4302537.879065/1‑94 (MQ=60)
                                                                                  AACCGACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGT          <  SRR4302537.5683421/94‑1 (MQ=60)
                                                                                      GACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCG      <  SRR4302537.1016826/94‑1 (MQ=60)
                                                                                      GACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCG      <  SRR4302537.893066/94‑1 (MQ=60)
                                                                                      GACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCG      <  SRR4302537.5476901/94‑1 (MQ=60)
                                                                                      GACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCG      <  SRR4302537.5362932/94‑1 (MQ=60)
                                                                                      GACACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCG      <  SRR4302537.488868/94‑1 (MQ=60)
                                                                                        CACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAGCGCGTTTCGAA    >  SRR4302537.4331949/1‑94 (MQ=60)
                                                                                        CACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGAC    >  SRR4302537.5450684/1‑94 (MQ=60)
                                                                                         ACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACA   <  SRR4302537.1573089/94‑1 (MQ=60)
                                                                                         ACCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACA   >  SRR4302537.2341817/1‑94 (MQ=60)
                                                                                          CCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAA  >  SRR4302537.2688130/1‑94 (MQ=60)
                                                                                          |                                                                                             
CCCGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAA  >  NC_000913/2668645‑2668828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑