Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,683,844	(A)_7→8	intergenic (‑317/‑11)	glyA ← / → hmpA	serine hydroxymethyltransferase/dihydropteridine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,683,837	1	.	A	99.6%	802.8 / ‑3.6	223	intergenic (‑310/‑18)	glyA/hmpA	serine hydroxymethyltransferase/dihydropteridine reductase
Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (116/106); total (116/107)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

Only 100 of 251 total aligned reads displayed.

TTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGC‑AAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGT  >  NC_000913/2683745‑2683924

ttCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGc                                                                                           <  2:4389103/93‑1 (MQ=255)

ttCATCACATTCTGTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGc                                                                                           <  2:670142/93‑1 (MQ=255)

 tcatcaCATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGC‑a                                                                                         <  2:4327064/93‑1 (MQ=255)

  catcaCATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGC‑aa                                                                                        >  2:3495184/1‑93 (MQ=255)

   atcaCATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGC‑aaa                                                                                       >  2:1592985/1‑93 (MQ=255)

      acaTTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGC‑aaaa‑aa                                                                                   <  1:5245236/93‑1 (MQ=255)

       caTTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGC‑aaaa‑aaa                                                                                  <  2:5670338/93‑1 (MQ=255)

        aTTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGC‑aaaa‑aaaa                                                                                 >  2:1265036/1‑92 (MQ=255)

        aTTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGC‑aaaa‑aaaa                                                                                 <  2:4605883/93‑2 (MQ=255)

         ttctttctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATATGAGATACATCAATTAAGTTGC‑aaaa‑aaaag                                                                                <  2:5168778/93‑3 (MQ=255)

          tctttctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGC‑aaaa‑aaaagg                                                                               >  2:4208113/1‑90 (MQ=255)

           ctttctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGC‑aaaa‑aaaagga                                                                              <  1:3821391/93‑5 (MQ=255)

             ttctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAg                                                                             >  1:1487505/1‑93 (MQ=255)

             ttctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAg                                                                             <  1:3022452/93‑1 (MQ=255)

             ttctGAAAAACACAAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAAATAAGATGCAAAAA‑AAAGGAAg                                                                             <  1:4639229/93‑1 (MQ=255)

              tctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGa                                                                            <  1:3344849/93‑1 (MQ=255)

               ctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGAc                                                                           <  1:3482705/93‑1 (MQ=255)

               ctGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGAc                                                                           <  1:929004/93‑1 (MQ=255)

                tGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGAcc                                                                          >  2:2679246/1‑93 (MQ=255)

                tGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGAcc                                                                          <  2:4453023/93‑1 (MQ=255)

                  aaaaaCACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCat                                                                        >  1:2703778/1‑93 (MQ=255)

                  aaaaaCACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCat                                                                        <  1:1626713/93‑1 (MQ=255)

                  aaaaaCACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCat                                                                        <  1:4269027/93‑1 (MQ=255)

                  aaaaaCACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCat                                                                        >  2:2893450/1‑93 (MQ=255)

                    aaaCACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCatat                                                                      <  2:4987246/93‑1 (MQ=255)

                    aaaCACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCatat                                                                      <  1:5632050/93‑1 (MQ=255)

                     aaCACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATg                                                                     <  1:4691318/93‑1 (MQ=255)

                      acacCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGc                                                                    <  2:769359/93‑1 (MQ=255)

                       cacCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCt                                                                   >  1:6427441/1‑93 (MQ=255)

                       cacCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCt                                                                   >  1:5765497/1‑93 (MQ=255)

                         ccAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTg                                                                 <  1:5930786/93‑1 (MQ=255)

                          cAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGa                                                                <  1:2451434/93‑1 (MQ=255)

                            aaGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACg                                                              >  2:3238525/1‑93 (MQ=255)

                             aGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGc                                                             >  2:113002/1‑93 (MQ=255)

                              gAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCt                                                            >  2:2492085/1‑93 (MQ=255)

                              gAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCt                                                            >  2:3027607/1‑93 (MQ=255)

                               aaCCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTc                                                           >  1:5315202/1‑93 (MQ=255)

                               aaCCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTc                                                           >  1:4600031/1‑93 (MQ=255)

                                aCCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCa                                                          >  2:4761168/1‑93 (MQ=255)

                                aCCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCa                                                          >  2:4933129/1‑93 (MQ=255)

                                  cATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCaaa                                                        >  2:2770748/1‑93 (MQ=255)

                                   aTTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAAc                                                       >  1:3057864/1‑93 (MQ=255)

                                   aTTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAAc                                                       <  2:5919002/93‑1 (MQ=255)

                                     ttACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCa                                                     >  2:1169029/1‑93 (MQ=255)

                                      tACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCAt                                                    <  2:927199/93‑1 (MQ=255)

                                       aCATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATc                                                   <  2:5444909/93‑1 (MQ=255)

                                         aTTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGc                                                 >  2:3633234/1‑93 (MQ=255)

                                         aTTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGc                                                 <  2:412076/93‑1 (MQ=255)

                                          ttGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCt                                                >  1:1152936/1‑93 (MQ=255)

                                           tGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTa                                               <  2:3946246/93‑1 (MQ=255)

                                            gCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTAc                                              <  1:2218248/93‑1 (MQ=255)

                                             cAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACa                                             <  2:3685999/93‑1 (MQ=255)

                                             cAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACa                                             <  1:1278781/93‑1 (MQ=255)

                                               gggCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGt                                           >  1:6178068/1‑93 (MQ=255)

                                                ggCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTa                                          >  2:2304773/1‑93 (MQ=255)

                                                 gCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTaa                                         <  1:6285027/93‑1 (MQ=255)

                                                 gCTATTTTTTATAAGATGCATTTGAGATACATCAATGAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTaa                                         <  1:3476748/93‑1 (MQ=255)

                                                  cTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTaaa                                        <  1:1424050/93‑1 (MQ=255)

                                                  cTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTaaa                                        <  2:2250237/93‑1 (MQ=255)

                                                   tATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTaaaa                                       >  1:4373846/1‑93 (MQ=255)

                                                   tATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTaaaa                                       >  1:2904648/1‑93 (MQ=255)

                                                    aTTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAg                                      >  1:3263667/1‑93 (MQ=255)

                                                     ttttttATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGc                                     <  1:5975611/93‑1 (MQ=255)

                                                     ttttttATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGc                                     <  1:6087313/93‑1 (MQ=255)

                                                      tttttATAAGATGCATTTGAGATACATGAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGcc                                    <  1:1901654/93‑1 (MQ=255)

                                                      tttttATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGcc                                    >  2:2051701/1‑93 (MQ=255)

                                                       ttttATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGcca                                   >  2:2136343/1‑93 (MQ=255)

                                                       ttttATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGcca                                   <  1:3959255/93‑1 (MQ=255)

                                                        tttATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGccac                                  <  2:1623125/93‑1 (MQ=255)

                                                         ttATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGccacc                                 >  2:1769374/1‑93 (MQ=255)

                                                          tataAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGccacca                                <  2:1395520/93‑1 (MQ=255)

                                                          tataAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGccacca                                <  2:4428792/93‑1 (MQ=255)

                                                           ataAGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCAt                               >  2:5470707/1‑93 (MQ=255)

                                                              aGATGCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATccc                            <  1:3945341/93‑1 (MQ=255)

                                                                  gCATTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTa                        <  1:3383610/93‑1 (MQ=255)

                                                                   gagttGAGATACATCAAGGAAGATGC‑AAAAGAAAGGAAGGCCATATGCTTGACGGTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTAc                       <  2:3128196/90‑1 (MQ=255)

                                                                    aTTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACt                      <  1:1071784/93‑1 (MQ=255)

                                                                    aTTTGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACt                      <  1:4458906/93‑1 (MQ=255)

                                                                     tttGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACtg                     >  1:4137528/1‑93 (MQ=255)

                                                                     tttGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACtg                     <  2:629058/93‑1 (MQ=255)

                                                                     tttGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACtg                     >  1:5405092/1‑93 (MQ=255)

                                                                     tttGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACtg                     <  1:5501845/93‑1 (MQ=255)

                                                                      ttGAGATACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACtgg                    <  1:4923953/93‑1 (MQ=255)

                                                                          gaTACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGa                <  1:151617/93‑1 (MQ=255)

                                                                           aTACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGaa               <  2:1879805/93‑1 (MQ=255)

                                                                            tACATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGaaa              <  2:6282668/93‑1 (MQ=255)

                                                                             aCATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAAc             <  1:6035051/93‑1 (MQ=255)

                                                                             aCATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAAc             <  2:2544487/93‑1 (MQ=255)

                                                                             aCATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAAc             <  2:5383238/93‑1 (MQ=255)

                                                                              cATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACg            >  1:4396804/1‑93 (MQ=255)

                                                                              cATCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACg            >  1:1688924/1‑93 (MQ=255)

                                                                               aTCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACgg           >  2:328106/1‑93 (MQ=255)

                                                                                tCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACggg          <  1:5935865/93‑1 (MQ=255)

                                                                                tCAATTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACggg          <  1:4099639/93‑1 (MQ=255)

                                                                                  aaTTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGc        <  2:4077315/93‑1 (MQ=255)

                                                                                   aTTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGcc       >  2:5071449/1‑93 (MQ=255)

                                                                                   aTTAAGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGcc       >  2:291232/1‑93 (MQ=255)

                                                                                      aaGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCaaa    <  2:1206061/93‑1 (MQ=255)

                                                                                      aaGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCaaa    >  1:5104516/1‑93 (MQ=255)

                                                                                       aGATGCAAAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAg   <  1:807204/93‑1 (MQ=255)

TTCATCACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGC‑AAAA‑AAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGT  >  NC_000913/2683745‑2683924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑C‑AAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTT  >  NC_000913/2683751‑2683937

ACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑C‑AAAAA‑AA                                                                                               <  SRR4302537.5281329/94‑1 (MQ=60)

 CATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑C‑AAAAA‑AAA                                                                                              >  SRR4302537.5865435/1‑94 (MQ=60)

  ATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑C‑AAAAA‑AAAG                                                                                             >  SRR4302537.283033/1‑94 (MQ=60)

   TTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑C‑AAAAA‑AAagg                                                                                            >  SRR4302537.2122916/1‑91 (MQ=60)

   TTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑C‑AAAAA‑AAagg                                                                                            >  SRR4302537.3980270/1‑91 (MQ=60)

   TTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑C‑AAAAA‑AAagg                                                                                            >  SRR4302537.3297202/1‑91 (MQ=60)

     CTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAA                                                                                           >  SRR4302537.4204321/1‑94 (MQ=60)

     CTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAA                                                                                           <  SRR4302537.3848185/94‑1 (MQ=60)

     CTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAA                                                                                           >  SRR4302537.1479230/1‑94 (MQ=60)

      TTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAG                                                                                          <  SRR4302537.2335137/94‑1 (MQ=60)

      TTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAG                                                                                          >  SRR4302537.1497856/1‑94 (MQ=60)

      TTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAG                                                                                          >  SRR4302537.1293371/1‑94 (MQ=60)

       TTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGA                                                                                         <  SRR4302537.3043521/94‑1 (MQ=60)

       TTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGA                                                                                         <  SRR4302537.4671311/94‑1 (MQ=60)

       TTCTGAAAAACACAAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAAATAAGATG‑CAAAAAA‑AAGGAAGA                                                                                         <  SRR4302537.4671312/94‑1 (MQ=60)

        TCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGAC                                                                                        <  SRR4302537.3368529/94‑1 (MQ=60)

         CTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACC                                                                                       >  SRR4302537.1486075/1‑94 (MQ=60)

         CTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACC                                                                                       <  SRR4302537.3507344/94‑1 (MQ=60)

         CTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACC                                                                                       <  SRR4302537.757589/94‑1 (MQ=60)

         CTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACC                                                                                       <  SRR4302537.935409/94‑1 (MQ=60)

           GAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCAT                                                                                     >  SRR4302537.2722656/1‑94 (MQ=60)

            AAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATA                                                                                    <  SRR4302537.4298688/94‑1 (MQ=60)

            AAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATA                                                                                    <  SRR4302537.4486343/94‑1 (MQ=60)

            AAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATA                                                                                    <  SRR4302537.1638089/94‑1 (MQ=60)

             AAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATAT                                                                                   <  SRR4302537.5172489/94‑1 (MQ=60)

              AAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATG                                                                                  <  SRR4302537.5670581/94‑1 (MQ=60)

               AACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGC                                                                                 <  SRR4302537.4723764/94‑1 (MQ=60)

                ACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCT                                                                                >  SRR4302537.4425039/1‑94 (MQ=60)

                ACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCT                                                                                <  SRR4302537.5008162/94‑1 (MQ=60)

                ACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCT                                                                                >  SRR4302537.5804990/1‑94 (MQ=60)

                ACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCT                                                                                >  SRR4302537.6471117/1‑94 (MQ=60)

                   CCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGA                                                                             >  SRR4302537.236199/1‑94 (MQ=60)

                   CCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGA                                                                             <  SRR4302537.5971477/94‑1 (MQ=60)

                    CAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGAC                                                                            >  SRR4302537.1693841/1‑94 (MQ=60)

                    CAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGAC                                                                            <  SRR4302537.2468680/94‑1 (MQ=60)

                    CAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACAATATGCTTGAC                                                                            >  SRR4302537.1085879/1‑94 (MQ=60)

                      AAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGC                                                                          >  SRR4302537.4756838/1‑94 (MQ=60)

                       AGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCT                                                                         <  SRR4302537.6162370/94‑1 (MQ=60)

                       AGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCT                                                                         >  SRR4302537.5348743/1‑94 (MQ=60)

                        GAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTC                                                                        >  SRR4302537.5351700/1‑94 (MQ=60)

                        GAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTC                                                                        >  SRR4302537.4631869/1‑94 (MQ=60)

                         AACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGATTGACGCTCA                                                                       >  SRR4302537.6128890/1‑94 (MQ=60)

                           CCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAA                                                                     >  SRR4302537.37139/1‑94 (MQ=60)

                           CCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAA                                                                     >  SRR4302537.5384478/1‑94 (MQ=60)

                            CATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAAC                                                                    >  SRR4302537.641105/1‑94 (MQ=60)

                            CATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAAC                                                                    >  SRR4302537.593154/1‑94 (MQ=60)

                            CATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAAC                                                                    >  SRR4302537.3079171/1‑94 (MQ=60)

                             ATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACC                                                                   >  SRR4302537.1597064/1‑94 (MQ=60)

                                TACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATC                                                                <  SRR4302537.3638279/94‑1 (MQ=60)

                                 ACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCG                                                               <  SRR4302537.2255934/94‑1 (MQ=60)

                                 ACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCG                                                               >  SRR4302537.6069615/1‑94 (MQ=60)

                                   ATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCT                                                             >  SRR4302537.1160959/1‑94 (MQ=60)

                                     TGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTAC                                                           >  SRR4302537.2756383/1‑94 (MQ=60)

                                      GCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACA                                                          <  SRR4302537.2233885/94‑1 (MQ=60)

                                       CAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAG                                                         >  SRR4302537.463326/1‑94 (MQ=60)

                                       CAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAG                                                         <  SRR4302537.1287665/94‑1 (MQ=60)

                                        AGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGT                                                        >  SRR4302537.6220618/1‑94 (MQ=60)

                                         GGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTA                                                       <  SRR4302537.3074726/94‑1 (MQ=60)

                                         GGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTA                                                       >  SRR4302537.5489544/1‑94 (MQ=60)

                                         GGGCTATTTTTTATAAGATGCATTTGAGATACATCAANTAAGATG‑C‑AAAAANAANGAAGACCANATGCTTGANGNTCAAANCNTCNCNNNANTA                                                       >  SRR4302537.288643/1‑94 (MQ=60)

                                          GGTTATTTTTAATAGGATGCATTGGAAATACATAAATAAAGATGAA‑AAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAA                                                      <  SRR4302537.888787/94‑1 (MQ=50)

                                           GCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAA                                                     <  SRR4302537.1521466/94‑1 (MQ=60)

                                           GCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAA                                                     <  SRR4302537.4354864/94‑1 (MQ=60)

                                           GCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAA                                                     >  SRR4302537.4795617/1‑94 (MQ=60)

                                           GCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAA                                                     <  SRR4302537.6328119/94‑1 (MQ=60)

                                           GCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAA                                                     <  SRR4302537.3472726/94‑1 (MQ=60)

                                           GCTATTTTTTATAAGATGCATTTGAGATACATCAATGAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAA                                                     <  SRR4302537.3501353/94‑1 (MQ=60)

                                            CTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAA                                                    <  SRR4302537.1433966/94‑1 (MQ=60)

                                            CTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAA                                                    >  SRR4302537.4404060/1‑94 (MQ=60)

                                            CTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAA                                                    >  SRR4302537.2925053/1‑94 (MQ=60)

                                            CTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAA                                                    >  SRR4302537.394981/1‑94 (MQ=60)

                                             TATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAG                                                   <  SRR4302537.4140165/94‑1 (MQ=60)

                                             TATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAG                                                   >  SRR4302537.3286689/1‑94 (MQ=60)

                                              ATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTATAAGC                                                  >  SRR4302537.4215557/1‑94 (MQ=60)

                                              ATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGC                                                  >  SRR4302537.6308360/1‑94 (MQ=60)

                                               TTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCC                                                 <  SRR4302537.6016635/94‑1 (MQ=60)

                                               TTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCC                                                 <  SRR4302537.6129131/94‑1 (MQ=60)

                                                TTTTTATAAGATGCATTTGAGATACATGAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCA                                                <  SRR4302537.1914860/94‑1 (MQ=60)

                                                 TTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCAC                                               <  SRR4302537.3986958/94‑1 (MQ=60)

                                                     ATAAGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATC                                           >  SRR4302537.4608467/1‑94 (MQ=60)

                                                        AGATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCT                                        <  SRR4302537.3972940/94‑1 (MQ=60)

                                                         GATGCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTT                                       <  SRR4302537.1275794/94‑1 (MQ=60)

                                                            GCATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTAC                                    <  SRR4302537.3407583/94‑1 (MQ=60)

                                                             CATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACT                                   >  SRR4302537.5589076/1‑94 (MQ=60)

                                                              ATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTG                                  >  SRR4302537.5441967/1‑94 (MQ=60)

                                                              ATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTG                                  >  SRR4302537.3170940/1‑94 (MQ=60)

                                                              ATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTG                                  >  SRR4302537.5614130/1‑94 (MQ=60)

                                                              ATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTG                                  <  SRR4302537.1079171/94‑1 (MQ=60)

                                                              ATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTG                                  >  SRR4302537.6323893/1‑94 (MQ=60)

                                                              ATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTG                                  <  SRR4302537.4489737/94‑1 (MQ=60)

                                                              ATTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTG                                  >  SRR4302537.4166391/1‑94 (MQ=60)

                                                               TTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGG                                 <  SRR4302537.5539414/94‑1 (MQ=60)

                                                               TTTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGG                                 >  SRR4302537.5396175/1‑94 (MQ=60)

                                                                TTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGT                                <  SRR4302537.1489331/94‑1 (MQ=60)

                                                                TTGAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGT                                <  SRR4302537.4957907/94‑1 (MQ=60)

                                                                  GAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGG                              <  SRR4302537.3344640/94‑1 (MQ=60)

                                                                  GAGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGG                              <  SRR4302537.40484/94‑1 (MQ=60)

                                                                   AGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCACACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGA                             >  SRR4302537.1828906/1‑94 (MQ=60)

                                                                   AGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGA                             >  SRR4302537.4475623/1‑94 (MQ=60)

                                                                   AGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGA                             <  SRR4302537.2950427/94‑1 (MQ=60)

                                                                   AGATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGA                             >  SRR4302537.4146391/1‑94 (MQ=60)

                                                                    GATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAA                            <  SRR4302537.152699/94‑1 (MQ=60)

                                                                    GATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAA                            >  SRR4302537.6015358/1‑94 (MQ=60)

                                                                    GATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAA                            >  SRR4302537.5976589/1‑94 (MQ=60)

                                                                    GATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCAACATCCCTTTACTGGTGGAA                            >  SRR4302537.3435266/1‑94 (MQ=60)

                                                                     ATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAA                           >  SRR4302537.2533253/1‑94 (MQ=60)

                                                                     ATACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAA                           >  SRR4302537.5696647/1‑94 (MQ=60)

                                                                       ACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACG                         <  SRR4302537.1187387/94‑1 (MQ=60)

                                                                       ACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACG                         >  SRR4302537.4309581/1‑94 (MQ=60)

                                                                       ACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACG                         <  SRR4302537.6076518/94‑1 (MQ=60)

                                                                       ACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACG                         >  SRR4302537.1700748/1‑94 (MQ=60)

                                                                       ACATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACG                         >  SRR4302537.4427139/1‑94 (MQ=60)

                                                                        CATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGG                        <  SRR4302537.1799941/94‑1 (MQ=60)

                                                                        CATCAATGAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGG                        <  SRR4302537.5989359/94‑1 (MQ=60)

                                                                         ATCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGG                       >  SRR4302537.3503341/1‑94 (MQ=60)

                                                                          TCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGG                      <  SRR4302537.5976600/94‑1 (MQ=60)

                                                                          TCAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGG                      <  SRR4302537.4128258/94‑1 (MQ=60)

                                                                           CAATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGC                     >  SRR4302537.3374822/1‑94 (MQ=60)

                                                                            AATTAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCC                    >  SRR4302537.521313/1‑94 (MQ=60)

                                                                               TAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAA                 <  SRR4302537.1538829/94‑1 (MQ=60)

                                                                               TAAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAA                 >  SRR4302537.5139697/1‑94 (MQ=60)

                                                                                AAGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAG                <  SRR4302537.4692866/94‑1 (MQ=60)

                                                                                 AGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGT               <  SRR4302537.812743/94‑1 (MQ=60)

                                                                                 AGATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGT               >  SRR4302537.704515/1‑94 (MQ=60)

                                                                                  agnancnanAAA‑AAGGAAGNCNNNATGCTTNACGCTTAANCCATCGCTACAGTAAANGCCACCATCCCNTTNCTGGTGGAAACGGGGCCAAAGT               <  SRR4302537.2209212/85‑1 (MQ=60)

                                                                                   ATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTA             <  SRR4302537.2192491/94‑1 (MQ=60)

                                                                                   ATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTA             >  SRR4302537.2375766/1‑94 (MQ=60)

                                                                                   ATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTA             <  SRR4302537.2899091/94‑1 (MQ=60)

                                                                                   ATG‑CAAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTA             >  SRR4302537.1595072/1‑94 (MQ=60)

                                                                                       caAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACC          <  SRR4302537.3840410/92‑1 (MQ=60)

                                                                                       caAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACC          <  SRR4302537.2442246/92‑1 (MQ=60)

                                                                                       caAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACC          <  SRR4302537.3314444/92‑1 (MQ=60)

                                                                                       caAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACC          <  SRR4302537.2083879/92‑1 (MQ=60)

                                                                                       caAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACC          >  SRR4302537.3997338/3‑94 (MQ=60)

                                                                                       caAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACC          <  SRR4302537.229135/92‑1 (MQ=60)

                                                                                       caAAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCACTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACC          >  SRR4302537.4851674/3‑94 (MQ=60)

                                                                                           AAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCC      >  SRR4302537.5399404/1‑94 (MQ=60)

                                                                                            AA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCA     <  SRR4302537.3953772/94‑1 (MQ=60)

                                                                                             A‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCAT    >  SRR4302537.2968132/1‑94 (MQ=60)

                                                                                             A‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCAT    >  SRR4302537.4380742/1‑94 (MQ=60)

                                                                                               AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATT   <  SRR4302537.3384559/94‑1 (MQ=60)

                                                                                                AGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTT  >  SRR4302537.4640428/1‑94 (MQ=60)

ACATTCTTTCTGAAAAACACCAAAGAACCATTTACATTGCAGGGCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATG‑C‑AAAAA‑AAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTT  >  NC_000913/2683751‑2683937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`