Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,688,395 G→A R263C (CGC→TGC)  yfhK ← putative 2‑component sensor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,688,3950GA94.9% 730.6 / 15.3 218R263C (CGC→TGC) yfhKputative 2‑component sensor protein
Reads supporting (aligned to +/- strand):  ref base G (4/7);  new base A (110/96);  total (114/104)
Fisher's exact test for biased strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 229 total aligned reads displayed.

GTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCC  >  NC_000913/2688304‑2688484
                                                                                           |                                                                                         
gTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGgcgc                                                                                          >  1:6444319/1‑93 (MQ=255)
    ccTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCAc                                                                                      <  2:882518/93‑1 (MQ=255)
     cTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGTGATTCCAGCCATGACAGGcactcact                                                                                     >  1:3419096/1‑93 (MQ=255)
      tCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACtt                                                                                    >  1:6114729/1‑93 (MQ=255)
      tCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACtt                                                                                    <  1:488261/93‑1 (MQ=255)
       cgcgCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTa                                                                                   >  1:740878/1‑93 (MQ=255)
        gcgcATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTaa                                                                                  <  2:3371911/93‑1 (MQ=255)
           cATACTCGCCAGTGGCGTTTTTAATTCCTGAGACAAATGTCTTAAAAATTAATGGCGCTGGGATTCCAGCCATGACAGGCACTCACTTAACCa                                                                               >  2:2187675/1‑93 (MQ=255)
             tACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCaaa                                                                             >  1:1255683/1‑93 (MQ=255)
             tACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCaaa                                                                             <  2:6191526/93‑1 (MQ=255)
             tACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCaaa                                                                             >  1:4688947/1‑93 (MQ=255)
                 cGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAAt                                                                         >  1:6243491/1‑93 (MQ=255)
                 cGCCAGTGCCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAAt                                                                         <  1:6412760/93‑1 (MQ=255)
                  gCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATa                                                                        <  2:1402342/93‑1 (MQ=255)
                  gCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATa                                                                        <  1:97501/93‑1 (MQ=255)
                  gCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATa                                                                        >  2:395441/1‑93 (MQ=255)
                  gCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATa                                                                        >  1:1714277/1‑93 (MQ=255)
                     aGTGGCTTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGt                                                                     <  2:5106931/93‑1 (MQ=255)
                     aGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGt                                                                     <  1:4908670/93‑1 (MQ=255)
                     aGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGt                                                                     >  2:4985393/1‑93 (MQ=255)
                     aGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGt                                                                     >  2:1776700/1‑93 (MQ=255)
                      gTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCAAGACAGGCACTCACTTAACCAAAGAATACGtt                                                                    <  2:1067464/93‑1 (MQ=255)
                       tGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTg                                                                   >  1:2419696/1‑93 (MQ=255)
                        ggCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGc                                                                  >  2:346198/1‑93 (MQ=255)
                            tttttAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCaa                                                              >  2:6464427/1‑93 (MQ=255)
                              tttAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAAcc                                                            >  1:267004/1‑93 (MQ=255)
                                tAATTCAGGAGATAAATGTCTTAAAATTTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGa                                                          <  2:2311991/93‑1 (MQ=255)
                                  aTTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGc                                                        >  2:6241577/1‑93 (MQ=255)
                                   ttCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCg                                                       <  1:3673319/93‑1 (MQ=255)
                                   ttCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCg                                                       >  1:3219090/1‑93 (MQ=255)
                                   ttCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCg                                                       <  1:2260417/93‑1 (MQ=255)
                                   ttCATGAGATAAATGTCTTAAAAATGGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCg                                                       <  1:3072362/93‑1 (MQ=255)
                                     cATGAGATAAACGTCTTAAAAAGTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTa                                                     <  1:1451640/93‑1 (MQ=255)
                                      aTGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCCACCGAGCGTaa                                                    >  2:3026350/1‑93 (MQ=255)
                                      aTGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTaa                                                    <  2:5458070/93‑1 (MQ=255)
                                      aTGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTaa                                                    <  1:1192650/93‑1 (MQ=255)
                                      aTGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTaa                                                    >  1:1323248/1‑93 (MQ=255)
                                       tGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAAc                                                   <  2:4603064/93‑1 (MQ=255)
                                        gagaTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAAct                                                  >  2:3575989/1‑93 (MQ=255)
                                        gagaTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAAct                                                  >  1:3887349/1‑93 (MQ=255)
                                         agaTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActc                                                 >  1:4339643/1‑93 (MQ=255)
                                         agaTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActc                                                 >  1:2767562/1‑93 (MQ=255)
                                         agaTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActc                                                 >  2:1336978/1‑93 (MQ=255)
                                         agaTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActc                                                 >  1:6181038/1‑93 (MQ=255)
                                         agaTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActc                                                 >  1:5942882/1‑93 (MQ=255)
                                         agaTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActc                                                 <  1:4799892/93‑1 (MQ=255)
                                          gaTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActcg                                                >  2:3068201/1‑93 (MQ=255)
                                           aTAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActcgc                                               <  2:1428802/93‑1 (MQ=255)
                                            tAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActcgct                                              >  2:1577274/1‑93 (MQ=255)
                                             aaaTGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActcgctc                                             <  1:150814/93‑1 (MQ=255)
                                             aaaTGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActcgctc                                             <  1:3037360/93‑1 (MQ=255)
                                              aaTGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActcgctcg                                            >  2:3733188/1‑93 (MQ=255)
                                              aaTGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActcgctcg                                            >  2:655336/1‑93 (MQ=255)
                                              aaTGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAActcgctcg                                            >  2:2182559/1‑93 (MQ=255)
                                                    ttAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCAc                                      <  1:3549282/93‑1 (MQ=255)
                                                    ttAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCAc                                      >  2:5704332/1‑93 (MQ=255)
                                                     tAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACt                                     <  1:2556418/93‑1 (MQ=255)
                                                      aaaaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTg                                    <  1:4405861/93‑1 (MQ=255)
                                                       aaaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGa                                   >  1:1648042/1‑93 (MQ=255)
                                                        aaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGaa                                  >  1:5308347/1‑93 (MQ=255)
                                                        aaaTTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGaa                                  <  2:4898965/93‑1 (MQ=255)
                                                         aaTTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAAc                                 <  2:1708713/93‑1 (MQ=255)
                                                         aaTTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAAc                                 <  1:4529256/93‑1 (MQ=255)
                                                           ttGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACga                               >  1:3370279/1‑93 (MQ=255)
                                                           ttGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACga                               >  2:2620601/1‑93 (MQ=255)
                                                           ttGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACga                               <  1:1745722/93‑1 (MQ=255)
                                                           ttGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACga                               <  2:2787660/93‑1 (MQ=255)
                                                           ttGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACga                               <  2:2901028/93‑1 (MQ=255)
                                                             gATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACgaga                             <  2:2293461/93‑1 (MQ=255)
                                                             gATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGCTCCACTGAACgaga                             >  2:1875747/1‑93 (MQ=255)
                                                              aTGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGAc                            >  1:5448510/1‑93 (MQ=255)
                                                              aTGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGAc                            >  1:1049073/1‑93 (MQ=255)
                                                              aTGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGAc                            >  2:274574/1‑93 (MQ=255)
                                                              aTGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGAc                            >  2:372374/1‑93 (MQ=255)
                                                               tGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACg                           >  2:4737606/1‑93 (MQ=255)
                                                                 gCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCt                         >  2:4240683/1‑93 (MQ=255)
                                                                 gCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCt                         >  1:5170568/1‑93 (MQ=255)
                                                                    ttGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTAtt                      >  2:6140519/1‑93 (MQ=255)
                                                                     tGGGATGCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTg                     <  2:2838145/93‑1 (MQ=255)
                                                                       ggATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCGACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGcc                   >  2:2695821/1‑93 (MQ=255)
                                                                       ggATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGcc                   >  1:5790885/1‑93 (MQ=255)
                                                                         aTTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCa                 <  2:3131594/93‑1 (MQ=255)
                                                                         aTTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCa                 <  2:5828449/93‑1 (MQ=255)
                                                                         aTTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCa                 <  2:5517563/93‑1 (MQ=255)
                                                                         aTTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCa                 >  2:3163754/1‑93 (MQ=255)
                                                                              aGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGaa            >  1:5664778/1‑93 (MQ=255)
                                                                              aGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGaa            <  1:1526795/93‑1 (MQ=255)
                                                                               gCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAAc           <  1:2386217/93‑1 (MQ=255)
                                                                               gCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAAc           <  1:5811955/93‑1 (MQ=255)
                                                                                 cATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGc         >  2:476271/1‑93 (MQ=255)
                                                                                 cATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGc         <  1:674720/93‑1 (MQ=255)
                                                                                 cATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGc         <  2:4132702/93‑1 (MQ=255)
                                                                                 cATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGc         <  1:400188/93‑1 (MQ=255)
                                                                                       aGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTcc   <  2:5361086/93‑1 (MQ=255)
                                                                                       aGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTcc   <  1:4069401/93‑1 (MQ=255)
                                                                                       aGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTcc   >  1:2848709/1‑93 (MQ=255)
                                                                                       aGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTcc   <  2:5312026/93‑1 (MQ=255)
                                                                                       aGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTcc   <  1:1842611/93‑1 (MQ=255)
                                                                                       aGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTcc   >  2:3929679/1‑93 (MQ=255)
                                                                                        ggCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTCCCCAGAGAACACCCTTccc  >  1:948085/1‑93 (MQ=255)
                                                                                           |                                                                                         
GTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCC  >  NC_000913/2688304‑2688484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAG  >  NC_000913/2688303‑2688488
                                                                                            |                                                                                             
AGTGCCCTCGCGCATACTCGCCAGTGGCTTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCAC                                                                                              <  SRR4302537.5728314/94‑1 (MQ=60)
AGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGC                                                                                              >  SRR4302537.6488040/1‑94 (MQ=60)
AGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCAC                                                                                              <  SRR4302537.3941465/94‑1 (MQ=60)
AGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCAC                                                                                              <  SRR4302537.6021144/94‑1 (MQ=60)
 GTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACT                                                                                             <  SRR4302537.6513719/94‑1 (MQ=60)
     gccctCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACT                                                                                         >  SRR4302537.1073749/6‑94 (MQ=60)
     CCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGTGATTCCAGCCATGACAGGCACTCACT                                                                                         >  SRR4302537.3443298/1‑94 (MQ=60)
      CTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTT                                                                                        >  SRR4302537.6156747/1‑94 (MQ=60)
       TCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTA                                                                                       <  SRR4302537.491614/94‑1 (MQ=60)
       TCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTA                                                                                       >  SRR4302537.745938/1‑94 (MQ=60)
       TCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTA                                                                                       <  SRR4302537.5843602/94‑1 (MQ=60)
         GCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAAC                                                                                     >  SRR4302537.1487986/1‑94 (MQ=60)
         GCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAAC                                                                                     <  SRR4302537.939627/94‑1 (MQ=60)
           GCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCA                                                                                   >  SRR4302537.1588661/1‑94 (MQ=60)
             ATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAA                                                                                 >  SRR4302537.4721382/1‑94 (MQ=60)
             ATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAA                                                                                 >  SRR4302537.1264417/1‑94 (MQ=60)
              TACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAG                                                                                <  SRR4302537.273438/94‑1 (MQ=60)
              TACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAG                                                                                <  SRR4302537.3086465/94‑1 (MQ=60)
                CTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAA                                                                              <  SRR4302537.678003/94‑1 (MQ=60)
                CTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAA                                                                              <  SRR4302537.1430742/94‑1 (MQ=60)
                 TCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAAT                                                                             >  SRR4302537.6286402/1‑94 (MQ=60)
                 TCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAAT                                                                             <  SRR4302537.2242651/94‑1 (MQ=60)
                  CGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATA                                                                            >  SRR4302537.1726245/1‑94 (MQ=60)
                  CGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATA                                                                            <  SRR4302537.1211618/94‑1 (MQ=60)
                  CGCCAGTGCCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATA                                                                            <  SRR4302537.6456381/94‑1 (MQ=60)
                   GCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATAC                                                                           <  SRR4302537.98210/94‑1 (MQ=60)
                    CCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACG                                                                          <  SRR4302537.5800414/94‑1 (MQ=60)
                      AGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTT                                                                        >  SRR4302537.1461303/1‑94 (MQ=60)
                      AGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTT                                                                        >  SRR4302537.2662136/1‑94 (MQ=60)
                      AGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTT                                                                        <  SRR4302537.4942550/94‑1 (MQ=60)
                       GTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTG                                                                       >  SRR4302537.2436749/1‑94 (MQ=60)
                        TGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGC                                                                      >  SRR4302537.4398722/1‑94 (MQ=60)
                        TGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGC                                                                      <  SRR4302537.3741090/94‑1 (MQ=60)
                         GGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCC                                                                     >  SRR4302537.1753095/1‑94 (MQ=60)
                         GGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCC                                                                     >  SRR4302537.6474439/1‑94 (MQ=60)
                           CGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCA                                                                   <  SRR4302537.1824421/94‑1 (MQ=60)
                             TTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAAC                                                                 >  SRR4302537.2803345/1‑94 (MQ=60)
                              TTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACC                                                                >  SRR4302537.4782388/1‑94 (MQ=60)
                              TTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACC                                                                >  SRR4302537.6164376/1‑94 (MQ=60)
                              TTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACC                                                                >  SRR4302537.268878/1‑94 (MQ=60)
                              TTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACC                                                                <  SRR4302537.6269351/94‑1 (MQ=60)
                              TTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACC                                                                >  SRR4302537.3405337/1‑94 (MQ=60)
                               TTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCG                                                               <  SRR4302537.5045620/94‑1 (MQ=60)
                               TTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCG                                                               <  SRR4302537.2984989/94‑1 (MQ=60)
                                TTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGA                                                              >  SRR4302537.4235325/1‑94 (MQ=60)
                                  AATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGC                                                            <  SRR4302537.548149/94‑1 (MQ=60)
                                  AATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGC                                                            >  SRR4302537.2440129/1‑94 (MQ=60)
                                   ATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCG                                                           >  SRR4302537.3241742/1‑94 (MQ=60)
                                   ATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCG                                                           <  SRR4302537.4878770/94‑1 (MQ=60)
                                   ATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCG                                                           >  SRR4302537.2069643/1‑94 (MQ=60)
                                   ATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCG                                                           >  SRR4302537.4587924/1‑94 (MQ=60)
                                    TTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGT                                                          <  SRR4302537.2276367/94‑1 (MQ=60)
                                    TTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGT                                                          <  SRR4302537.3699162/94‑1 (MQ=60)
                                    TTCATGAGATAAATGTCTTAAAAATGGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGT                                                          <  SRR4302537.3093765/94‑1 (MQ=60)
                                      CATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAA                                                        >  SRR4302537.1332472/1‑94 (MQ=60)
                                      CATGAGATAAACGTCTTAAAAAGTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAA                                                        <  SRR4302537.1461755/94‑1 (MQ=60)
                                       ATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAAC                                                       <  SRR4302537.1200933/94‑1 (MQ=60)
                                        TGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACT                                                      >  SRR4302537.3914540/1‑94 (MQ=60)
                                         GAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTC                                                     >  SRR4302537.4369755/1‑94 (MQ=60)
                                         GAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTC                                                     >  SRR4302537.5983668/1‑94 (MQ=60)
                                         GAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTC                                                     >  SRR4302537.6223615/1‑94 (MQ=60)
                                         GAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTC                                                     >  SRR4302537.2786905/1‑94 (MQ=60)
                                          AGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCG                                                    <  SRR4302537.349021/94‑1 (MQ=60)
                                          AGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCG                                                    <  SRR4302537.4833008/94‑1 (MQ=60)
                                          AGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCG                                                    >  SRR4302537.4272163/1‑94 (MQ=60)
                                           GATAAATGTCTTAAAAATTGATGGCGTTGGGATCCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGC                                                   <  SRR4302537.304098/94‑1 (MQ=60)
                                            ATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCT                                                  >  SRR4302537.3668918/1‑94 (MQ=60)
                                              AAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCG                                                <  SRR4302537.151891/94‑1 (MQ=60)
                                              AAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCG                                                <  SRR4302537.3058528/94‑1 (MQ=60)
                                               AATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGG                                               <  SRR4302537.2414805/94‑1 (MQ=60)
                                                   TCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCA                                           >  SRR4302537.721767/1‑94 (MQ=60)
                                                    CTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCAC                                          >  SRR4302537.3144857/1‑94 (MQ=60)
                                                     TTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACT                                         <  SRR4302537.4520273/94‑1 (MQ=60)
                                                     TTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACT                                         <  SRR4302537.3574351/94‑1 (MQ=60)
                                                      TAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTG                                        <  SRR4302537.2574339/94‑1 (MQ=60)
                                                       AAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGA                                       <  SRR4302537.4436264/94‑1 (MQ=60)
                                                       AAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGA                                       >  SRR4302537.1659581/1‑94 (MQ=60)
                                                        AAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAA                                      >  SRR4302537.5344820/1‑94 (MQ=60)
                                                         AAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAAC                                     <  SRR4302537.5516771/94‑1 (MQ=60)
                                                         AAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAAC                                     >  SRR4302537.152960/1‑94 (MQ=60)
                                                         AAATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAAC                                     <  SRR4302537.872888/94‑1 (MQ=60)
                                                          AATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACG                                    <  SRR4302537.4560617/94‑1 (MQ=60)
                                                          AATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACG                                    >  SRR4302537.6336690/1‑94 (MQ=60)
                                                           ATTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGA                                   >  SRR4302537.3394160/1‑94 (MQ=60)
                                                            TTGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAG                                  <  SRR4302537.1757897/94‑1 (MQ=60)
                                                             TGATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGA                                 <  SRR4302537.330908/94‑1 (MQ=60)
                                                              GATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGAC                                >  SRR4302537.1612660/1‑94 (MQ=60)
                                                              GATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGAC                                >  SRR4302537.1056307/1‑94 (MQ=60)
                                                              GATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGAC                                >  SRR4302537.5485674/1‑94 (MQ=60)
                                                              GATGGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGAC                                >  SRR4302537.4931520/1‑94 (MQ=60)
                                                                 GGCGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCT                             >  SRR4302537.5206172/1‑94 (MQ=60)
                                                                   CGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACTAGACGCTAT                           <  SRR4302537.4787998/94‑1 (MQ=60)
                                                                   CGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTAT                           >  SRR4302537.3919398/1‑94 (MQ=60)
                                                                   CGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTAT                           <  SRR4302537.2453873/94‑1 (MQ=60)
                                                                   CGTTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTAT                           >  SRR4302537.170691/1‑94 (MQ=60)
                                                                    GTGGGGATCCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATT                          <  SRR4302537.3704992/94‑1 (MQ=60)
                                                                     TTGGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTG                         <  SRR4302537.4994107/94‑1 (MQ=60)
                                                                       GGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCC                       >  SRR4302537.5252678/1‑94 (MQ=60)
                                                                       GGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCC                       >  SRR4302537.5996065/1‑94 (MQ=60)
                                                                       GGGATTCCAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCC                       >  SRR4302537.5830590/1‑94 (MQ=60)
                                                                              CAGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAA                >  SRR4302537.5703534/1‑94 (MQ=60)
                                                                               CGCCATGACAGGCCCTCACTTAACCAACGAATACGTTGCCAAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGCCGCTATTGCCCAGAGAAC               <  SRR4302537.4541247/94‑1 (MQ=60)
                                                                               AGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAAC               <  SRR4302537.1537460/94‑1 (MQ=60)
                                                                               AGCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAAC               <  SRR4302537.405618/94‑1 (MQ=60)
                                                                                GCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACG              <  SRR4302537.5851799/94‑1 (MQ=60)
                                                                                GCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACG              <  SRR4302537.2403024/94‑1 (MQ=60)
                                                                                GCCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACG              <  SRR4302537.5651995/94‑1 (MQ=60)
                                                                                 CCATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGC             >  SRR4302537.3325607/1‑94 (MQ=60)
                                                                                  CATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCC            <  SRR4302537.402963/94‑1 (MQ=60)
                                                                                  CATGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCC            <  SRR4302537.679338/94‑1 (MQ=60)
                                                                                    TGACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCT          <  SRR4302537.1289525/94‑1 (MQ=60)
                                                                                     GACAGGCACTCACTTAACCAAAGAATACGTTGCCCAGCCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCACTT         <  SRR4302537.5899970/94‑1 (MQ=60)
                                                                                     GACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTT         >  SRR4302537.2321440/1‑94 (MQ=60)
                                                                                      ACAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTC        <  SRR4302537.6082592/94‑1 (MQ=60)
                                                                                       CAGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCC       >  SRR4302537.2868657/1‑94 (MQ=60)
                                                                                        AGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCC      <  SRR4302537.1855436/94‑1 (MQ=60)
                                                                                        AGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCC      >  SRR4302537.6453694/1‑94 (MQ=60)
                                                                                        AGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCC      <  SRR4302537.4097825/94‑1 (MQ=60)
                                                                                        AGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCC      <  SRR4302537.973184/94‑1 (MQ=60)
                                                                                        AGGCACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTCCCCAGAGAACACCCTTCCC      >  SRR4302537.954643/1‑94 (MQ=60)
                                                                                           CACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCA   <  SRR4302537.4856498/94‑1 (MQ=60)
                                                                                            ACTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAG  <  SRR4302537.2814583/94‑1 (MQ=60)
                                                                                            |                                                                                             
AGTGCCCTCGCGCATACTCGCCAGTGGCGTTTTTAATTCATGAGATAAATGTCTTAAAAATTGATGGCGTTGGGATTCCAGCCATGACAGGCGCTCACTTAACCAAAGAATACGTTGCCCAACCGAGCGTAACTCGCTCGGTCCACTGAACGAGACGCTATTGCCCAGAGAACGCCCTTCCCCCAG  >  NC_000913/2688303‑2688488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑