Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,552	C→T	G129G (GGC→GGT)	b2595 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,552	0	C	T	95.7%	723.4 / 10.9	209	G129G (GGC→GGT)	b2595	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (5/4); new base T (95/105); total (100/109)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

Only 100 of 218 total aligned reads displayed.

CCCGACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGT  >  NC_000913/2734462‑2734640

cccGACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGgtgt                                                                                        >  2:3820726/1‑93 (MQ=255)

 ccGACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTc                                                                                       <  2:3744007/93‑1 (MQ=255)

 ccGACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGAGTc                                                                                       >  2:3319908/1‑93 (MQ=255)

   gACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGa                                                                                     >  1:4948152/1‑93 (MQ=255)

   caCCCATCAGAATATCGATGATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGa                                                                                     <  1:1235017/92‑1 (MQ=255)

    aCCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGAt                                                                                    >  1:6160035/1‑93 (MQ=255)

      ccATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCg                                                                                  >  1:3056524/1‑93 (MQ=255)

      ccATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCg                                                                                  >  1:2579090/1‑93 (MQ=255)

      ccATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCg                                                                                  >  2:5945210/1‑93 (MQ=255)

       cATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGt                                                                                 >  2:1675200/1‑93 (MQ=255)

       cATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGt                                                                                 >  2:6388700/1‑93 (MQ=255)

        aTCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTa                                                                                <  2:3003771/93‑1 (MQ=255)

        aTCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCCCTGGATGACAGTGCCCTGCAAGGCTTCTTTGCTGACGATCgcg                                                                                >  1:1792342/1‑91 (MQ=255)

         tCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAg                                                                               >  1:5165412/1‑93 (MQ=255)

          ccGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgc                                                                              <  2:1240971/93‑1 (MQ=255)

          ccGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgc                                                                              <  1:2155372/93‑1 (MQ=255)

           cGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcg                                                                             >  1:5799884/1‑93 (MQ=255)

            gAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcga                                                                            >  2:3890807/1‑93 (MQ=255)

            gAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcga                                                                            <  1:2644488/93‑1 (MQ=255)

             aaTATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAgcgat                                                                           <  1:4478644/93‑1 (MQ=255)

              atatCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcgatc                                                                          <  1:4766031/93‑1 (MQ=255)

              atatCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcgatc                                                                          <  2:1100972/93‑1 (MQ=255)

               tatCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcgatcg                                                                         >  1:1401943/1‑93 (MQ=255)

               tatCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcgatcg                                                                         >  2:2314316/1‑93 (MQ=255)

                atCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcgatcgc                                                                        <  2:3679222/93‑1 (MQ=255)

                atCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcgatcgc                                                                        <  1:2063999/93‑1 (MQ=255)

                atCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcgatcgc                                                                        <  1:5356108/93‑1 (MQ=255)

                 tCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcgatcgcg                                                                       <  2:5682968/93‑1 (MQ=255)

                 tCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcgatcgcg                                                                       >  2:5487397/1‑93 (MQ=255)

                 tCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcgatcgcg                                                                       >  1:4124711/1‑93 (MQ=255)

                  cGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAgcgatcgcga                                                                      <  2:4677423/93‑1 (MQ=255)

                       aTGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCGATCGCGATCCTc                                                                 <  2:2549949/93‑1 (MQ=255)

                        tGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCa                                                                >  1:2929918/1‑93 (MQ=255)

                        tGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCa                                                                <  1:650599/93‑1 (MQ=255)

                          tCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAAc                                                              <  2:6027699/93‑1 (MQ=255)

                          tCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAAc                                                              >  2:598362/1‑93 (MQ=255)

                           cATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACa                                                             <  2:631703/93‑1 (MQ=255)

                           cATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACa                                                             <  2:5072656/93‑1 (MQ=255)

                           cATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACa                                                             <  1:5916294/93‑1 (MQ=255)

                            aTGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACAt                                                            <  2:341281/93‑1 (MQ=255)

                             tGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATg                                                           >  2:4404310/1‑93 (MQ=255)

                             tGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATg                                                           <  1:5047883/93‑1 (MQ=255)

                             tGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCGATCGCGATCCTCAACATg                                                           >  1:4827099/1‑93 (MQ=255)

                              gTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGc                                                          <  1:568947/93‑1 (MQ=255)

                               tACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCa                                                         >  2:1137520/1‑93 (MQ=255)

                               tACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCa                                                         <  2:2393201/93‑1 (MQ=255)

                                    gCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGc                                                    <  2:4880505/93‑1 (MQ=255)

                                    gCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGc                                                    >  2:1244830/1‑93 (MQ=255)

                                     ggTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCt                                                   <  1:3987337/92‑1 (MQ=255)

                                      gTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTg                                                  >  2:5251166/1‑93 (MQ=255)

                                      gTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTAAACATGCACGAGCTg                                                  <  1:1924018/93‑1 (MQ=255)

                                       tGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGc                                                 <  2:5593041/93‑1 (MQ=255)

                                       gggCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGc                                                 <  1:2766590/92‑1 (MQ=255)

                                         gCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGt                                               >  2:1383718/1‑93 (MQ=255)

                                         gCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGt                                               <  2:1900787/93‑1 (MQ=255)

                                          ccTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGtt                                              <  1:5466595/93‑1 (MQ=255)

                                           cTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGttt                                             <  1:1470542/93‑1 (MQ=255)

                                           cTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGttt                                             >  1:3550125/1‑93 (MQ=255)

                                            tGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTa                                            >  1:3749600/1‑93 (MQ=255)

                                              aCCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGt                                          <  2:4246150/93‑1 (MQ=255)

                                               ccAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAgcg                                         <  2:6185007/93‑3 (MQ=255)

                                               ccAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTg                                         <  2:2072145/93‑1 (MQ=255)

                                                cAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGa                                        >  1:4233959/1‑93 (MQ=255)

                                                cAATATGGCGCTGGATGACAGTGCGATGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCATCAACATGCACGAGCTGCGTTTAGTGa                                        <  2:2380884/93‑1 (MQ=255)

                                                 aaTATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGAc                                       >  1:1968756/1‑93 (MQ=255)

                                                 aaTATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGAc                                       <  2:5226668/93‑1 (MQ=255)

                                                 aaTATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTACGTTTAGTGat                                       >  1:1166210/1‑92 (MQ=255)

                                                   tatGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACtt                                     >  2:3078996/1‑93 (MQ=255)

                                                    atGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACttt                                    <  1:2972076/93‑1 (MQ=255)

                                                      ggCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTc                                  <  1:983153/93‑1 (MQ=255)

                                                        cgcTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCa                                <  1:3682121/93‑1 (MQ=255)

                                                           tGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAAc                             <  1:1707559/93‑1 (MQ=255)

                                                             gATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTg                           <  2:4174128/93‑1 (MQ=255)

                                                              aTGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTgg                          <  1:2729277/93‑1 (MQ=255)

                                                              aTGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTgg                          >  1:1385050/1‑93 (MQ=255)

                                                               tGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGt                         <  2:1545942/93‑1 (MQ=255)

                                                               tGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTACACACTggc                         >  2:1463992/1‑92 (MQ=255)

                                                                 aCAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTgc                       <  1:3814532/93‑1 (MQ=255)

                                                                  cAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTgcg                      >  1:5437472/1‑93 (MQ=255)

                                                                   aGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTgcgc                     >  1:260923/1‑93 (MQ=255)

                                                                    gTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGcgcg                    >  2:2809942/1‑93 (MQ=255)

                                                                     tGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCgg                   >  2:5732807/1‑93 (MQ=255)

                                                                      gcgcTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGc                  >  1:4205691/1‑93 (MQ=255)

                                                                       cgcTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCt                 <  1:3491943/93‑1 (MQ=255)

                                                                        gcTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTa                <  1:3200300/93‑1 (MQ=255)

                                                                           gCAAGTGTTCTTTGGTGCCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATcc             <  2:2230492/93‑1 (MQ=255)

                                                                            cAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCg            >  2:655444/1‑93 (MQ=255)

                                                                            cAAGGGTTCTTTGGCGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCg            <  2:4991843/93‑1 (MQ=255)

                                                                             aaGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGa           >  1:1639481/1‑93 (MQ=255)

                                                                             aaGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGa           >  1:2330499/1‑93 (MQ=255)

                                                                              aGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGaa          >  2:67172/1‑93 (MQ=255)

                                                                              aGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGaa          >  2:3203956/1‑93 (MQ=255)

                                                                              aGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGaa          <  2:209448/93‑1 (MQ=255)

                                                                               gggTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAAc         >  1:779389/1‑93 (MQ=255)

                                                                                ggTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAAca        >  1:1309307/1‑93 (MQ=255)

                                                                                 gTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAAcag       >  1:4702445/1‑93 (MQ=255)

                                                                                  ttCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAAcagt      >  2:3570060/1‑93 (MQ=255)

                                                                                  ttCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAAcagt      >  2:1578696/1‑93 (MQ=255)

                                                                                      ttGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAAcagtcagt  <  2:5792318/93‑1 (MQ=255)

                                                                                      ttGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAAcagtcagt  <  1:6312853/93‑1 (MQ=255)

CCCGACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGT  >  NC_000913/2734462‑2734640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAACCCGACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTA  >  NC_000913/2734459‑2734641

TAACCCGACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGT                                                                                           <  SRR4302537.2017139/94‑1 (MQ=60)

 AACCCGACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTG                                                                                          <  SRR4302537.2797549/94‑1 (MQ=60)

   CCCGACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTC                                                                                        >  SRR4302537.997591/1‑94 (MQ=60)

   CCCGACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTC                                                                                        >  SRR4302537.488139/1‑94 (MQ=60)

     CGACCCATCCGAATATCGATTATGTCATGTAGATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGA                                                                                      >  SRR4302537.6061130/1‑94 (MQ=60)

     CGACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGA                                                                                      >  SRR4302537.4982264/1‑94 (MQ=60)

      GACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGAT                                                                                     >  SRR4302537.5236644/1‑94 (MQ=60)

      GACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGAT                                                                                     >  SRR4302537.6202429/1‑94 (MQ=60)

      CACCCATCAGAATATCGATGATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGAT                                                                                     <  SRR4302537.1243604/94‑1 (MQ=60)

        CCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCG                                                                                   >  SRR4302537.3077818/1‑94 (MQ=60)

        CCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCG                                                                                   <  SRR4302537.2102821/94‑1 (MQ=60)

        CCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCG                                                                                   >  SRR4302537.2597144/1‑94 (MQ=60)

          CATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTA                                                                                 >  SRR4302537.1956428/1‑94 (MQ=60)

           ATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAG                                                                                >  SRR4302537.2635077/1‑94 (MQ=60)

           ATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAG                                                                                >  SRR4302537.5201003/1‑94 (MQ=60)

           ATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAG                                                                                >  SRR4302537.1402562/1‑94 (MQ=60)

             CCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCG                                                                              <  SRR4302537.2170562/94‑1 (MQ=60)

             CCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCG                                                                              >  SRR4302537.5839648/1‑94 (MQ=60)

              CGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGA                                                                             >  SRR4302537.1336032/1‑94 (MQ=60)

               GAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGAT                                                                            <  SRR4302537.2662985/94‑1 (MQ=60)

               GAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGAT                                                                            <  SRR4302537.760051/94‑1 (MQ=60)

               GAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCGAT                                                                            <  SRR4302537.2004586/94‑1 (MQ=60)

                AATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCGATC                                                                           <  SRR4302537.4509625/94‑1 (MQ=60)

                 ATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCG                                                                          <  SRR4302537.4798946/94‑1 (MQ=60)

                 ATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCG                                                                          >  SRR4302537.1411710/1‑94 (MQ=60)

                 ATATCGATTATGTCATGTACATACGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCG                                                                          <  SRR4302537.2775363/94‑1 (MQ=60)

                  TATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGC                                                                         <  SRR4302537.3072394/94‑1 (MQ=60)

                   ATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCG                                                                        <  SRR4302537.2078491/94‑1 (MQ=60)

                   ATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCG                                                                        <  SRR4302537.5392794/94‑1 (MQ=60)

                   ATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCG                                                                        >  SRR4302537.5437728/1‑94 (MQ=60)

                   ATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCG                                                                        >  SRR4302537.4153500/1‑94 (MQ=60)

                     CGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGAT                                                                      <  SRR4302537.5581063/94‑1 (MQ=60)

                      GATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATC                                                                     <  SRR4302537.1738021/94‑1 (MQ=60)

                       ATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCC                                                                    >  SRR4302537.1828957/1‑94 (MQ=60)

                          ATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCA                                                                 >  SRR4302537.2950492/1‑94 (MQ=60)

                           TGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAA                                                                >  SRR4302537.4600501/1‑94 (MQ=60)

                           TGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAA                                                                <  SRR4302537.655028/94‑1 (MQ=60)

                             TCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACA                                                              <  SRR4302537.2332067/94‑1 (MQ=60)

                              CATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACAT                                                             <  SRR4302537.5956890/94‑1 (MQ=60)

                               ATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATG                                                            <  SRR4302537.2160785/94‑1 (MQ=60)

                               ATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATG                                                            <  SRR4302537.905004/94‑1 (MQ=60)

                               ATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATG                                                            <  SRR4302537.3526107/94‑1 (MQ=60)

                               ATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATG                                                            >  SRR4302537.1493162/1‑94 (MQ=60)

                               ATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATG                                                            >  SRR4302537.1696382/1‑94 (MQ=60)

                               ATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCGATCGCGATCCTCAACATG                                                            >  SRR4302537.4860442/1‑94 (MQ=60)

                               ATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCGATCGCGATCCTCAACATG                                                            >  SRR4302537.3612090/1‑94 (MQ=60)

                                TGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGC                                                           <  SRR4302537.5082643/94‑1 (MQ=60)

                                TGTACATGCGGGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGC                                                           <  SRR4302537.3977772/94‑1 (MQ=60)

                                 GTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCA                                                          >  SRR4302537.2559984/1‑94 (MQ=60)

                                 GTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCA                                                          <  SRR4302537.572858/94‑1 (MQ=60)

                                 GTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCA                                                          <  SRR4302537.6027658/94‑1 (MQ=60)

                                  TACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCAC                                                         <  SRR4302537.2901990/94‑1 (MQ=60)

                                       GCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTATGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCT                                                    <  SRR4302537.3480703/94‑1 (MQ=60)

                                        GGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTG                                                   <  SRR4302537.4015264/94‑1 (MQ=60)

                                        CGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTG                                                   <  SRR4302537.2955979/94‑1 (MQ=60)

                                         GTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTAAACATGCACGAGCTGC                                                  <  SRR4302537.1937373/94‑1 (MQ=60)

                                          GGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCG                                                 <  SRR4302537.2785929/94‑1 (MQ=60)

                                            GCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTT                                               <  SRR4302537.5189883/94‑1 (MQ=60)

                                             CCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTT                                              >  SRR4302537.3575199/1‑94 (MQ=60)

                                             CCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTT                                              <  SRR4302537.5503882/94‑1 (MQ=60)

                                              CTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTA                                             >  SRR4302537.3775932/1‑94 (MQ=60)

                                              CTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTA                                             <  SRR4302537.1480764/94‑1 (MQ=60)

                                              CTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTA                                             >  SRR4302537.1176094/1‑94 (MQ=60)

                                               TGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAG                                            <  SRR4302537.4375266/94‑1 (MQ=60)

                                               TGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAG                                            <  SRR4302537.2699366/94‑1 (MQ=60)

                                               TGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAG                                            >  SRR4302537.4082674/1‑94 (MQ=60)

                                               TGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAG                                            <  SRR4302537.5892436/94‑1 (MQ=60)

                                                GACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGT                                           >  SRR4302537.4212762/1‑94 (MQ=60)

                                                GACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGT                                           <  SRR4302537.2599250/94‑1 (MQ=60)

                                                 ACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTG                                          <  SRR4302537.5475255/94‑1 (MQ=60)

                                                 ACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTG                                          <  SRR4302537.6410829/94‑1 (MQ=60)

                                                  CCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGA                                         >  SRR4302537.4263424/1‑94 (MQ=60)

                                                   CAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGAC                                        >  SRR4302537.1982448/1‑94 (MQ=60)

                                                   CAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGAC                                        <  SRR4302537.3153354/94‑1 (MQ=60)

                                                   CAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTACGTTTAGTGAT                                        >  SRR4302537.1174319/1‑94 (MQ=60)

                                                       ATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTT                                    <  SRR4302537.2992900/94‑1 (MQ=60)

                                                         GGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCC                                  <  SRR4302537.989946/94‑1 (MQ=60)

                                                           CGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAA                                <  SRR4302537.3708018/94‑1 (MQ=60)

                                                              TGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACT                             <  SRR4302537.1719495/94‑1 (MQ=60)

                                                              TGGATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACT                             <  SRR4302537.1604844/94‑1 (MQ=60)

                                                                GATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGG                           >  SRR4302537.1394711/1‑94 (MQ=60)

                                                                 ATGACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGT                          <  SRR4302537.2748333/94‑1 (MQ=60)

                                                                 ATGACAGTCCGCTGCACGGCTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGT                          <  SRR4302537.3763343/94‑1 (MQ=60)

                                                                   GACAGTGCGTTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGC                        <  SRR4302537.3960618/94‑1 (MQ=60)

                                                                   GACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGC                        <  SRR4302537.5054730/94‑1 (MQ=60)

                                                                    ACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCG                       <  SRR4302537.3841280/94‑1 (MQ=60)

                                                                    ACAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCG                       >  SRR4302537.5474563/1‑94 (MQ=60)

                                                                     CAGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGC                      >  SRR4302537.262762/1‑94 (MQ=60)

                                                                      AGTGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCG                     >  SRR4302537.5799493/1‑94 (MQ=60)

                                                                        TGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGC                   >  SRR4302537.48192/1‑94 (MQ=60)

                                                                        TGCGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGC                   >  SRR4302537.4235041/1‑94 (MQ=60)

                                                                          CGCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTA                 <  SRR4302537.3516643/94‑1 (MQ=60)

                                                                           GCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTAT                <  SRR4302537.3222799/94‑1 (MQ=60)

                                                                           GCTGCAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTAT                <  SRR4302537.479832/94‑1 (MQ=60)

                                                                               CAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGA            >  SRR4302537.1650965/1‑94 (MQ=60)

                                                                               CAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGA            >  SRR4302537.6277451/1‑94 (MQ=60)

                                                                               CAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGA            >  SRR4302537.3839349/1‑94 (MQ=60)

                                                                               CAAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGA            >  SRR4302537.2346932/1‑94 (MQ=60)

                                                                                AAGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAA           >  SRR4302537.474800/1‑94 (MQ=60)

                                                                                 AGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAAC          >  SRR4302537.784738/1‑94 (MQ=60)

                                                                                 AGGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAAC          >  SRR4302537.426319/1‑94 (MQ=60)

                                                                                 AGGGTTCTTTGGTGTCGATCGTAGCGATAGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAAC          >  SRR4302537.3263846/1‑94 (MQ=60)

                                                                                  GGGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACA         >  SRR4302537.1318421/1‑94 (MQ=60)

                                                                                   GGTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAG        >  SRR4302537.4734977/1‑94 (MQ=60)

                                                                                    GTTCTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGT       <  SRR4302537.5252906/94‑1 (MQ=60)

                                                                                       CTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAG    >  SRR4302537.2686387/1‑94 (MQ=60)

                                                                                       CTTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAG    <  SRR4302537.1300807/94‑1 (MQ=60)

                                                                                        TTTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGT   >  SRR4302537.145393/1‑94 (MQ=60)

                                                                                         TTGGTGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTA  <  SRR4302537.6356064/94‑1 (MQ=60)

TAACCCGACCCATCCGAATATCGATTATGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTCGATCGTAGCGATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTA  >  NC_000913/2734459‑2734641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`