Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,770,753 T→C intergenic (‑578/+104) b2638 ← / ← b4541 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,770,7530TC99.5% 751.0 / ‑5.0 219intergenic (‑578/+104)b2638/b4541hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (97/121);  minor base A (0/1);  total (97/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

Only 100 of 234 total aligned reads displayed.

CCGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCTCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTC  >  NC_000913/2770663‑2770842
                                                                                          |                                                                                         
ccGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGcccc                                                                                         <  2:931240/93‑1 (MQ=255)
 cGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCa                                                                                        <  1:2775919/93‑1 (MQ=255)
 cGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCa                                                                                        >  1:1483407/1‑93 (MQ=255)
 cGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCa                                                                                        <  2:2633021/93‑1 (MQ=255)
  ggTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCag                                                                                       >  1:496304/1‑93 (MQ=255)
  ggTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCag                                                                                       >  1:3499536/1‑93 (MQ=255)
  ggTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCag                                                                                       <  1:2125542/93‑1 (MQ=255)
  ggTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCACGATTCCCCTCGGcccccc                                                                                       >  2:5736053/1‑91 (MQ=255)
   gtgtTTATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCaga                                                                                      <  2:2340656/93‑1 (MQ=255)
   gtgtTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCaga                                                                                      <  1:5383090/93‑1 (MQ=255)
   gtgtTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCaga                                                                                      >  2:3288691/1‑93 (MQ=255)
      ttGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCagaaag                                                                                   >  1:5672670/1‑93 (MQ=255)
       tGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCagaaaga                                                                                  <  1:4896003/93‑1 (MQ=255)
        gATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCagaaagaa                                                                                 >  2:6363896/1‑93 (MQ=255)
        gATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCagaaagaa                                                                                 <  2:757568/93‑1 (MQ=255)
        gATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCagaaagaa                                                                                 >  2:1274452/1‑93 (MQ=255)
         aTCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAaagaag                                                                                <  1:3279340/93‑1 (MQ=255)
           cGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAgaagaa                                                                              <  1:2842929/93‑1 (MQ=255)
            gAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGaaa                                                                             >  1:5750532/1‑93 (MQ=255)
             aaTGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAAc                                                                            <  2:426864/93‑1 (MQ=255)
              aTGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAAcc                                                                           <  1:3743419/93‑1 (MQ=255)
               tGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCg                                                                          <  2:6432982/93‑1 (MQ=255)
                gACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGa                                                                         >  2:1719949/1‑93 (MQ=255)
                gACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGa                                                                         <  2:5684747/93‑1 (MQ=255)
                 aCCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCGGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGAc                                                                        <  2:6250338/93‑1 (MQ=255)
                 aCCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGAc                                                                        <  1:2030091/93‑1 (MQ=255)
                 aCCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGAc                                                                        <  1:3032380/93‑1 (MQ=255)
                 aCCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGAc                                                                        >  2:3114225/1‑93 (MQ=255)
                   ctctTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACaa                                                                      <  1:4061292/93‑1 (MQ=255)
                   ctctTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACaa                                                                      >  1:4331433/1‑93 (MQ=255)
                   ctcgTTTCGCCCCCTCAAACAGCACTGCTGCGCCAGCGGCCGCGATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACaa                                                                      <  2:3260454/93‑1 (MQ=255)
                    tctTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAag                                                                     >  2:6084334/1‑93 (MQ=255)
                      ttttCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAagca                                                                   >  1:3909182/1‑93 (MQ=255)
                       tttCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAagcag                                                                  <  2:389161/93‑1 (MQ=255)
                         tCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAgcagca                                                                <  1:6183999/93‑1 (MQ=255)
                          cGCCACCACAAACAGCACTGCTGCGCCAGCGGACGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCaa                                                               <  2:1641695/93‑1 (MQ=255)
                            ccaccaCAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATa                                                             >  1:3006656/1‑93 (MQ=255)
                             caccacAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATaa                                                            >  1:3812014/1‑93 (MQ=255)
                             caccacAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATaa                                                            >  2:6146123/1‑93 (MQ=255)
                             caccacAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATaa                                                            >  1:6339030/1‑93 (MQ=255)
                              accacAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAAc                                                           >  2:4018849/1‑93 (MQ=255)
                              accacAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAAc                                                           <  2:2809552/93‑1 (MQ=255)
                               ccacAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACa                                                          <  1:1704787/93‑1 (MQ=255)
                               ccacAAACAGCACTGCTGCGCCAGCGGCAGCTGTCGCACTGAGCAGGATTCCCTGCGGCCCCCGAAAGAAGAAACCGCAGAACAGCAATAAcg                                                          >  2:1213167/1‑92 (MQ=255)
                                cacaACCAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAg                                                         <  1:1586149/93‑1 (MQ=255)
                                cacaACCAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAg                                                         <  1:479526/93‑1 (MQ=255)
                                cacaAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAg                                                         <  1:5563067/93‑1 (MQ=255)
                                cacaAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAg                                                         <  2:4428326/93‑1 (MQ=255)
                                cacaAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAg                                                         <  1:4430642/93‑1 (MQ=255)
                                  caAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGa                                                       <  2:1350901/93‑1 (MQ=255)
                                   aaaCAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGAc                                                      >  2:3368465/1‑93 (MQ=255)
                                   aaaCAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGAc                                                      >  2:5493033/1‑93 (MQ=255)
                                   aaaCAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGAc                                                      <  2:5244894/93‑1 (MQ=255)
                                     aCAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACaa                                                    <  1:1613302/93‑1 (MQ=255)
                                     aCAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACaa                                                    <  2:1525098/93‑1 (MQ=255)
                                     aCAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACaa                                                    <  2:4744612/93‑1 (MQ=255)
                                       aGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATc                                                  >  1:1021147/1‑93 (MQ=255)
                                         cACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCa                                                <  2:3748627/93‑1 (MQ=255)
                                          aCTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAg                                               <  1:114206/93‑1 (MQ=255)
                                           ctgctgCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAgc                                              <  1:1439470/93‑1 (MQ=255)
                                           ctgctgCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAgc                                              <  1:5959061/93‑1 (MQ=255)
                                               tgcGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAgcccgc                                          >  2:2276293/1‑93 (MQ=255)
                                                gcgcCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAgcccgcc                                         >  2:806023/1‑93 (MQ=255)
                                                gcgcCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAgcccgcc                                         <  1:614533/93‑1 (MQ=255)
                                                 cgcCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAgcccgccc                                        <  1:2954712/93‑1 (MQ=255)
                                                 cgcCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAgcccgccc                                        >  1:1893122/1‑93 (MQ=255)
                                                 cgcCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCAAGAAAGAAGAACCCGACAAGCAGCAATAACAGGACAATCCAgcccgccc                                        <  2:2398863/93‑1 (MQ=255)
                                                   ccAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTg                                      >  2:910647/1‑93 (MQ=255)
                                                    cAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGa                                     >  1:2497603/1‑93 (MQ=255)
                                                     aGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGaa                                    <  2:198417/93‑1 (MQ=255)
                                                     aGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGaa                                    >  1:5546269/1‑93 (MQ=255)
                                                      gCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAg                                   <  1:2178711/93‑1 (MQ=255)
                                                           cGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGc                              <  1:905556/93‑1 (MQ=255)
                                                             cTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCa                            <  1:658027/93‑1 (MQ=255)
                                                             cTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCa                            >  1:1449572/1‑93 (MQ=255)
                                                                tCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGAt                         >  2:1407012/1‑93 (MQ=255)
                                                                tCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGAt                         >  2:4455274/1‑93 (MQ=255)
                                                                tCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGAt                         >  1:3344019/1‑93 (MQ=255)
                                                                  gCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATcc                       >  2:4151633/1‑93 (MQ=255)
                                                                    aCTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCtt                     >  2:360556/1‑93 (MQ=255)
                                                                     cTGACCAGGATTCCCTGCGGCCGCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGATGCCAGATCCTTg                    >  2:784673/1‑93 (MQ=255)
                                                                     cTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTg                    <  2:5088197/93‑1 (MQ=255)
                                                                      tGACCAGGATCCCCTGCGCCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGa                   <  2:5819272/93‑1 (MQ=255)
                                                                       gACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGAt                  <  1:2425868/93‑1 (MQ=255)
                                                                        aCCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATc                 <  2:4927018/93‑1 (MQ=255)
                                                                        aCCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATc                 >  2:5019396/1‑93 (MQ=255)
                                                                          cAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGc               >  1:5273113/1‑93 (MQ=255)
                                                                           aGGATTCCCTGCGGCCCCAGCAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGGCCTGAAGGTTGCCAGATCCTTGATCGCa              >  1:59306/1‑93 (MQ=255)
                                                                            ggATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCAc             <  1:3947332/93‑1 (MQ=255)
                                                                            ggATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCAc             >  1:3796354/1‑93 (MQ=255)
                                                                            ggATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCAc             <  2:1814916/93‑1 (MQ=255)
                                                                            ggATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGAAAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCAc             <  2:63033/93‑1 (MQ=255)
                                                                             taTTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACt            <  2:2085736/92‑1 (MQ=255)
                                                                              aTTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTg           >  1:5326547/1‑93 (MQ=255)
                                                                              aTTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTg           <  2:1103411/93‑1 (MQ=255)
                                                                                    tGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGc     <  1:2366623/93‑1 (MQ=255)
                                                                                    tGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGc     <  2:1793813/93‑1 (MQ=255)
                                                                                     gCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCg    >  1:1220790/1‑93 (MQ=255)
                                                                                       ggCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTc  <  2:5326088/93‑1 (MQ=255)
                                                                                       ggCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTc  <  1:5086495/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCTCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTC  >  NC_000913/2770663‑2770842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCTCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCTTCA  >  NC_000913/2770662‑2770846
                                                                                           |                                                                                             
TCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCC                                                                                             >  SRR4302537.5242283/1‑94 (MQ=60)
 CCGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCA                                                                                            >  SRR4302537.3360451/1‑94 (MQ=60)
 CCGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCA                                                                                            >  SRR4302537.1493724/1‑94 (MQ=60)
  CGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAG                                                                                           >  SRR4302537.3524297/1‑94 (MQ=60)
  CGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAG                                                                                           <  SRR4302537.2795324/94‑1 (MQ=60)
  CGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAG                                                                                           <  SRR4302537.3278790/94‑1 (MQ=60)
  CGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAG                                                                                           >  SRR4302537.499717/1‑94 (MQ=60)
   GGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGA                                                                                          <  SRR4302537.2140495/94‑1 (MQ=60)
    GTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAA                                                                                         <  SRR4302537.5419896/94‑1 (MQ=60)
      GTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAG                                                                                       >  SRR4302537.5711485/1‑94 (MQ=60)
      GTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAG                                                                                       <  SRR4302537.3017779/94‑1 (MQ=60)
       TTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGA                                                                                      >  SRR4302537.1748141/1‑94 (MQ=60)
       TTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCACGATTCCCTGCGGCCCCAGAAAGA                                                                                      >  SRR4302537.562392/1‑94 (MQ=60)
        TGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAA                                                                                     <  SRR4302537.4929806/94‑1 (MQ=60)
         GATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAG                                                                                    >  SRR4302537.2666076/1‑94 (MQ=60)
          ATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGA                                                                                   <  SRR4302537.3302489/94‑1 (MQ=60)
          ATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGA                                                                                   <  SRR4302537.3048166/94‑1 (MQ=60)
           TCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAA                                                                                  >  SRR4302537.1607691/1‑94 (MQ=60)
           TCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAA                                                                                  <  SRR4302537.4287756/94‑1 (MQ=60)
            CGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAA                                                                                 >  SRR4302537.572760/1‑94 (MQ=60)
            CGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAA                                                                                 >  SRR4302537.1887986/1‑94 (MQ=60)
            CGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAA                                                                                 <  SRR4302537.2862828/94‑1 (MQ=60)
            CGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAA                                                                                 >  SRR4302537.5789923/1‑94 (MQ=60)
              AATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACC                                                                               <  SRR4302537.3947724/94‑1 (MQ=60)
               ATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCG                                                                              <  SRR4302537.3769705/94‑1 (MQ=60)
                TGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGA                                                                             >  SRR4302537.2445697/1‑94 (MQ=60)
                  ACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACA                                                                           <  SRR4302537.2044245/94‑1 (MQ=60)
                  ACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACA                                                                           <  SRR4302537.3053523/94‑1 (MQ=60)
                  ACCTCTTTTCGCCACCAAAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACA                                                                           <  SRR4302537.4975871/94‑1 (MQ=60)
                   CCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAA                                                                          <  SRR4302537.1527063/94‑1 (MQ=60)
                   CCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAA                                                                          >  SRR4302537.4361521/1‑94 (MQ=60)
                    CTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAG                                                                         <  SRR4302537.6083397/94‑1 (MQ=60)
                    CTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAG                                                                         <  SRR4302537.4089670/94‑1 (MQ=60)
                     TCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGC                                                                        >  SRR4302537.5731255/1‑94 (MQ=60)
                     TCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGC                                                                        >  SRR4302537.3844764/1‑94 (MQ=60)
                      CTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCA                                                                       >  SRR4302537.1448524/1‑94 (MQ=60)
                      CTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCA                                                                       >  SRR4302537.3936508/1‑94 (MQ=60)
                         TGCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCA                                                                    <  SRR4302537.2459996/94‑1 (MQ=60)
                          TCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAA                                                                   <  SRR4302537.6226597/94‑1 (MQ=60)
                           CGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAAT                                                                  >  SRR4302537.3542944/1‑94 (MQ=60)
                           CGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAAT                                                                  <  SRR4302537.5376103/94‑1 (MQ=60)
                           CGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAAT                                                                  >  SRR4302537.4364865/1‑94 (MQ=60)
                            GCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATA                                                                 >  SRR4302537.3027592/1‑94 (MQ=60)
                             CCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAA                                                                >  SRR4302537.3838740/1‑94 (MQ=60)
                             CCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAA                                                                >  SRR4302537.1194989/1‑94 (MQ=60)
                             CCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAA                                                                >  SRR4302537.6382349/1‑94 (MQ=60)
                                CCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAG                                                             <  SRR4302537.1716706/94‑1 (MQ=60)
                                 CACAACCAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGG                                                            <  SRR4302537.1597237/94‑1 (MQ=60)
                                 CACAACCAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGG                                                            <  SRR4302537.482813/94‑1 (MQ=60)
                                 CACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGG                                                            <  SRR4302537.4792843/94‑1 (MQ=60)
                                 CACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGG                                                            <  SRR4302537.5601088/94‑1 (MQ=60)
                                 CACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGG                                                            <  SRR4302537.4461240/94‑1 (MQ=60)
                                 CACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGG                                                            >  SRR4302537.6204550/1‑94 (MQ=60)
                                  ACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGA                                                           <  SRR4302537.844865/94‑1 (MQ=60)
                                   CAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGAC                                                          <  SRR4302537.3474747/94‑1 (MQ=60)
                                     AACAGCACTGCTGCGCCAGCGGCAGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAA                                                        >  SRR4302537.2186415/1‑94 (MQ=60)
                                      ACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAAT                                                       >  SRR4302537.2150942/1‑94 (MQ=60)
                                      ACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAAT                                                       <  SRR4302537.1624578/94‑1 (MQ=60)
                                       CAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATC                                                      >  SRR4302537.1028184/1‑94 (MQ=60)
                                       CAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATC                                                      <  SRR4302537.5298605/94‑1 (MQ=60)
                                        AGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCC                                                     <  SRR4302537.4901630/94‑1 (MQ=60)
                                        AGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCC                                                     >  SRR4302537.2026362/1‑94 (MQ=60)
                                        AGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCA                                                     >  SRR4302537.146035/1‑94 (MQ=60)
                                          CACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACATGACAATTCAG                                                   >  SRR4302537.1457133/1‑94 (MQ=60)
                                          CACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAG                                                   <  SRR4302537.5496350/94‑1 (MQ=60)
                                          CACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAG                                                   <  SRR4302537.983186/94‑1 (MQ=60)
                                           ACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGC                                                  >  SRR4302537.3771829/1‑94 (MQ=60)
                                           ACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGC                                                  <  SRR4302537.115038/94‑1 (MQ=60)
                                            CTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCC                                                 >  SRR4302537.2810196/1‑94 (MQ=60)
                                            CTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCC                                                 <  SRR4302537.5999963/94‑1 (MQ=60)
                                            CTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCC                                                 >  SRR4302537.3868949/1‑94 (MQ=60)
                                            CTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCC                                                 <  SRR4302537.1449502/94‑1 (MQ=60)
                                             TGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCC                                                <  SRR4302537.1041166/94‑1 (MQ=60)
                                             TGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCAACAAGCAGCAATAACAGGACAATCCAGCCC                                                <  SRR4302537.5526035/94‑1 (MQ=60)
                                              GCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCG                                               <  SRR4302537.6241162/94‑1 (MQ=60)
                                                 GCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCC                                            >  SRR4302537.1906278/1‑94 (MQ=60)
                                                 GCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCC                                            <  SRR4302537.618722/94‑1 (MQ=60)
                                                  CGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCT                                           <  SRR4302537.2121747/94‑1 (MQ=60)
                                                  CGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCT                                           <  SRR4302537.2975446/94‑1 (MQ=60)
                                                  CGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAAAAGGACAATCCAGCCCGCCCT                                           <  SRR4302537.638836/94‑1 (MQ=60)
                                                    CCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGA                                         >  SRR4302537.2515182/1‑94 (MQ=60)
                                                     CAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAA                                        >  SRR4302537.5584140/1‑94 (MQ=60)
                                                     CAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAA                                        <  SRR4302537.5986976/94‑1 (MQ=60)
                                                      AGCGTCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAG                                       <  SRR4302537.159407/94‑1 (MQ=60)
                                                       GCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGG                                      <  SRR4302537.2194066/94‑1 (MQ=60)
                                                         GGCCGCTATCGCACTGACCAGGATTCCCTTCGGCCCCAGAAAGAAGAACGCGACAAGCAGCAATAACAGGACAGTCCAGCCCGCCCTGAAGGTT                                    <  SRR4302537.739917/94‑1 (MQ=60)
                                                         GGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTT                                    >  SRR4302537.5361809/1‑94 (MQ=60)
                                                         GGCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCACTGAAGGTG                                    >  SRR4302537.2295248/1‑94 (MQ=60)
                                                          GCCGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTG                                   <  SRR4302537.5584736/94‑1 (MQ=60)
                                                            CGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCC                                 <  SRR4302537.3127016/94‑1 (MQ=60)
                                                            CGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCC                                 >  SRR4302537.6405004/1‑94 (MQ=60)
                                                            CGCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCC                                 <  SRR4302537.911809/94‑1 (MQ=60)
                                                             GCTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCA                                >  SRR4302537.1459671/1‑94 (MQ=60)
                                                              CTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAG                               <  SRR4302537.1687074/94‑1 (MQ=60)
                                                              CTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAG                               <  SRR4302537.662513/94‑1 (MQ=60)
                                                              CTATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAG                               <  SRR4302537.3832472/94‑1 (MQ=60)
                                                               TATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGA                              >  SRR4302537.2870864/1‑94 (MQ=60)
                                                               TATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGA                              >  SRR4302537.1244996/1‑94 (MQ=60)
                                                                ATCGCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGAT                             >  SRR4302537.3367689/1‑94 (MQ=60)
                                                                   GCACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCT                          <  SRR4302537.4009496/94‑1 (MQ=60)
                                                                    CACTGACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTT                         <  SRR4302537.2398047/94‑1 (MQ=60)
                                                                        GACCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATC                     <  SRR4302537.2442961/94‑1 (MQ=60)
                                                                          CCAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGC                   >  SRR4302537.5309400/1‑94 (MQ=60)
                                                                           CAGGATTCCCTGCGGCCCCAGCAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGGCCTGAAGGTTGCCAGATCCTTGATCGCA                  >  SRR4302537.59743/1‑94 (MQ=60)
                                                                           CAGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTaacgcaa                  >  SRR4302537.3462069/1‑87 (MQ=60)
                                                                            AGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCAC                 >  SRR4302537.3822991/1‑94 (MQ=60)
                                                                            AGGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCAC                 <  SRR4302537.4861324/94‑1 (MQ=60)
                                                                             GGATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACT                <  SRR4302537.3974940/94‑1 (MQ=60)
                                                                              GATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTG               >  SRR4302537.93680/1‑94 (MQ=60)
                                                                              GATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTG               >  SRR4302537.5363094/1‑94 (MQ=60)
                                                                               ATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGG              <  SRR4302537.288453/94‑1 (MQ=60)
                                                                               ATTCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGG              <  SRR4302537.5014843/94‑1 (MQ=60)
                                                                                 TCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCA            <  SRR4302537.4255257/94‑1 (MQ=60)
                                                                                 TCCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCA            <  SRR4302537.3552120/94‑1 (MQ=60)
                                                                                  CCCTGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAG           >  SRR4302537.6048575/1‑94 (MQ=60)
                                                                                   CCTGCGGCCACAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGG          <  SRR4302537.586584/94‑1 (MQ=60)
                                                                                     TGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCG        <  SRR4302537.2383306/94‑1 (MQ=60)
                                                                                     TGCGGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCG        >  SRR4302537.1229279/1‑94 (MQ=60)
                                                                                        GGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGgat     >  SRR4302537.1712976/1‑91 (MQ=60)
                                                                                        GGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCT     >  SRR4302537.3460854/1‑94 (MQ=60)
                                                                                        GGCCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCT     <  SRR4302537.5121512/94‑1 (MQ=60)
                                                                                          CCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCTTC   <  SRR4302537.706034/94‑1 (MQ=60)
                                                                                          CCCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCTTC   >  SRR4302537.3790725/1‑94 (MQ=60)
                                                                                           CCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCTTCA  >  SRR4302537.2802981/1‑94 (MQ=60)
                                                                                           |                                                                                             
TCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACAAACAGCACTGCTGCGCCAGCGGCCGCTATCGCACTGACCAGGATTCCCTGCGGCTCCAGAAAGAAGAAACCGACAAGCAGCAATAACAGGACAATCCAGCCCGCCCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCTTCA  >  NC_000913/2770662‑2770846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑