Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,821,290 G→A A168V (GCG→GTG)  recA ← RecA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,821,2900GA97.0% 910.4 / 7.7 262A168V (GCG→GTG) recARecA
Reads supporting (aligned to +/- strand):  ref base G (6/2);  new base A (139/115);  total (145/117)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

Only 100 of 264 total aligned reads displayed.

ATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTG  >  NC_000913/2821200‑2821380
                                                                                          |                                                                                          
aTACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCcaca                                                                                          >  1:2473900/1‑93 (MQ=255)
aTACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCcaca                                                                                          >  2:2806493/1‑93 (MQ=255)
  aCGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAg                                                                                        <  1:3125895/93‑1 (MQ=255)
    ggATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGc                                                                                      >  1:6175348/1‑93 (MQ=255)
    ggATCTGGTGGATGAAGATCAGCAGCGTGTTGCACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGc                                                                                      <  1:6035616/93‑1 (MQ=255)
      aTCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGccc                                                                                    >  1:275643/1‑93 (MQ=255)
       tCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCa                                                                                   <  1:3246367/93‑1 (MQ=255)
       tCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCa                                                                                   <  2:1070918/93‑1 (MQ=255)
        cTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCAt                                                                                  <  1:1806150/93‑1 (MQ=255)
         tGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCAtg                                                                                 >  2:1655553/1‑93 (MQ=255)
         tGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCAtg                                                                                 >  1:5520270/1‑93 (MQ=255)
         tGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCAtg                                                                                 >  1:5057605/1‑93 (MQ=255)
         tGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCAtg                                                                                 <  2:5141288/93‑1 (MQ=255)
         tGGTTGATGAAGATCAGCAGCGTGTGGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCAtg                                                                                 <  1:5751094/93‑1 (MQ=255)
            ttGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTga                                                                              <  2:5460195/93‑1 (MQ=255)
            ttGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTga                                                                              <  1:508742/93‑1 (MQ=255)
            ttGATAAAGATCAGCAGCGTGTTTGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTga                                                                              <  2:1003091/93‑1 (MQ=255)
             tgatgaAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTgag                                                                             <  2:1299970/93‑1 (MQ=255)
             tgatgaAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTgag                                                                             <  2:5011838/93‑1 (MQ=255)
              gatgaAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTgaga                                                                            >  2:1337419/1‑93 (MQ=255)
              gatgaAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTgaga                                                                            >  2:1404596/1‑93 (MQ=255)
                tgaAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTTAGAGt                                                                          >  1:2212147/1‑93 (MQ=255)
                tgaAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGt                                                                          <  1:3008761/93‑1 (MQ=255)
                      tCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGa                                                                    >  2:3221765/1‑93 (MQ=255)
                      tCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGa                                                                    <  2:4657570/93‑1 (MQ=255)
                       cagcagCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGAt                                                                   <  1:1939718/93‑1 (MQ=255)
                       cagcagCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGAt                                                                   <  2:2957906/93‑1 (MQ=255)
                         acagcGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGAttt                                                                 <  1:1304593/92‑1 (MQ=255)
                           agcGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCg                                                               >  1:3607700/1‑93 (MQ=255)
                           agcGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCg                                                               >  1:6218375/1‑93 (MQ=255)
                              gtgtTGGCCTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCt                                                            <  1:15259/93‑1 (MQ=255)
                              gtgtTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCt                                                            >  1:5212840/1‑93 (MQ=255)
                                gtTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGTCTTc                                                          >  2:4487638/1‑93 (MQ=255)
                                  tGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGa                                                        <  2:4850786/93‑1 (MQ=255)
                                    gACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGAtt                                                      >  2:1842723/1‑93 (MQ=255)
                                     aCTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTAGCCTTCGAttt                                                     >  2:5260078/1‑93 (MQ=255)
                                      cTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTc                                                    >  2:5641356/1‑93 (MQ=255)
                                       tGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTcc                                                   <  2:6124374/93‑1 (MQ=255)
                                       tGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTcc                                                   <  2:4327039/93‑1 (MQ=255)
                                        gCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCg                                                  >  2:3611500/1‑93 (MQ=255)
                                           tCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCtt                                               >  1:2527659/1‑93 (MQ=255)
                                              gggtACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCg                                            <  1:1651177/90‑1 (MQ=255)
                                              ggTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCg                                            >  2:5448402/1‑93 (MQ=255)
                                              ggTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTCCCACAAGGCCCCTGTGAGAGTCGCCGCTTTAGCCTTCGATTTACGCTTTCg                                            >  1:3916879/1‑93 (MQ=255)
                                               gTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCgg                                           >  1:245491/1‑93 (MQ=255)
                                               gTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCgg                                           >  1:2945678/1‑93 (MQ=255)
                                               gTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCgg                                           <  2:4016097/93‑1 (MQ=255)
                                               gTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCgg                                           <  2:5671544/93‑1 (MQ=255)
                                               gTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCgg                                           >  1:4814652/1‑93 (MQ=255)
                                               gTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCgg                                           <  1:4419571/93‑1 (MQ=255)
                                                ttccccGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGc                                          <  2:306942/90‑1 (MQ=255)
                                                ttccccGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGc                                          <  1:3332578/90‑1 (MQ=255)
                                                 tACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCg                                         <  1:4964844/93‑1 (MQ=255)
                                                 tACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCg                                         <  1:4880404/93‑1 (MQ=255)
                                                 tACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCg                                         <  2:6402319/93‑1 (MQ=255)
                                                   cccGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTc                                       >  1:6402363/1‑93 (MQ=255)
                                                    ccGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCa                                      >  1:1226386/1‑93 (MQ=255)
                                                    ccGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCa                                      >  1:3645462/1‑93 (MQ=255)
                                                     cGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGTCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAg                                     <  1:4049091/93‑1 (MQ=255)
                                                     cGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAg                                     >  1:5790986/1‑93 (MQ=255)
                                                       ccAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTg                                   <  2:5233990/93‑1 (MQ=255)
                                                       ccAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTAGCCTTCGATTTGCGCTTTCGGCGTCAGTg                                   >  1:535607/1‑93 (MQ=255)
                                                        cAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTgc                                  >  2:868121/1‑93 (MQ=255)
                                                        cAGCTTACGCATCAGCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCAGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTgc                                  <  1:2040568/93‑1 (MQ=255)
                                                         aGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTgcc                                 <  1:4185488/93‑1 (MQ=255)
                                                            ttACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTgccgcc                              >  1:430460/1‑93 (MQ=255)
                                                             tACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCa                             >  1:1084940/1‑93 (MQ=255)
                                                             tACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCa                             <  2:4535045/93‑1 (MQ=255)
                                                             tACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCa                             <  1:4785859/93‑1 (MQ=255)
                                                             tACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCa                             >  2:838931/1‑93 (MQ=255)
                                                              aCGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCAc                            <  2:2558818/93‑1 (MQ=255)
                                                               cGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACg                           >  2:5547250/1‑93 (MQ=255)
                                                                 cATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGa                         >  1:5655947/1‑93 (MQ=255)
                                                                 cATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGa                         >  2:4056546/1‑93 (MQ=255)
                                                                  aTCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAg                        >  2:1479892/1‑93 (MQ=255)
                                                                  aTCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAg                        >  2:6049946/1‑93 (MQ=255)
                                                                   tCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGt                       <  1:2894233/93‑1 (MQ=255)
                                                                   tCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGt                       <  2:3149366/93‑1 (MQ=255)
                                                                   tCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGt                       >  1:5933740/1‑93 (MQ=255)
                                                                   tCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGt                       <  1:1492645/93‑1 (MQ=255)
                                                                     gCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCa                     >  2:5908497/1‑93 (MQ=255)
                                                                      ccTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCaa                    <  2:3320005/93‑1 (MQ=255)
                                                                        tGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAacg                  >  1:2625669/1‑93 (MQ=255)
                                                                         ggCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAacga                 >  1:1746953/1‑93 (MQ=255)
                                                                         ggCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAacga                 <  2:1929094/93‑1 (MQ=255)
                                                                         ggCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAacga                 <  2:3914888/93‑1 (MQ=255)
                                                                           cTCATCATACGTGACACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAacgacg               <  2:972673/93‑1 (MQ=255)
                                                                              atcatACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATa            >  1:3783547/1‑93 (MQ=255)
                                                                               tcatACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATaa           <  2:4740088/93‑1 (MQ=255)
                                                                                catACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAAc          <  2:4615341/93‑1 (MQ=255)
                                                                                 atACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACg         >  1:892875/1‑93 (MQ=255)
                                                                                 atACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACg         >  2:2675099/1‑93 (MQ=255)
                                                                                  tACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGt        <  2:4915129/93‑1 (MQ=255)
                                                                                  tACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGt        >  2:4122789/1‑93 (MQ=255)
                                                                                  tACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGt        >  1:657886/1‑93 (MQ=255)
                                                                                     gTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTa     <  2:4442922/93‑1 (MQ=255)
                                                                                     gTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTa     <  2:2320253/93‑1 (MQ=255)
                                                                                      tGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTAc    >  2:3795840/1‑93 (MQ=255)
                                                                                      tGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTAc    >  2:2035284/1‑93 (MQ=255)
                                                                                      ggCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTAc    <  1:4952687/92‑1 (MQ=255)
                                                                                          |                                                                                          
ATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTG  >  NC_000913/2821200‑2821380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCG  >  NC_000913/2821198‑2821382
                                                                                            |                                                                                            
TCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCAC                                                                                             >  SRR4302537.4478098/1‑94 (MQ=60)
 CATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACA                                                                                            <  SRR4302537.4152402/94‑1 (MQ=60)
 CATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACA                                                                                            >  SRR4302537.2491292/1‑94 (MQ=60)
   TACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAG                                                                                          <  SRR4302537.3607409/94‑1 (MQ=60)
   TACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAG                                                                                          <  SRR4302537.3272203/94‑1 (MQ=60)
   TACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAG                                                                                          <  SRR4302537.3699654/94‑1 (MQ=60)
    ACGGATCTGGTTTATTAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGG                                                                                         <  SRR4302537.2694093/94‑1 (MQ=60)
    ACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGG                                                                                         <  SRR4302537.3147793/94‑1 (MQ=60)
     CGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGC                                                                                        >  SRR4302537.6217878/1‑94 (MQ=60)
      GGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCC                                                                                       <  SRR4302537.5117486/94‑1 (MQ=60)
      GGATCTGGTGGATGAAGATCAGCAGCGTGTTGCACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCC                                                                                       <  SRR4302537.6077089/94‑1 (MQ=60)
       GATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCC                                                                                      >  SRR4302537.277574/1‑94 (MQ=60)
        ATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCA                                                                                     >  SRR4302537.5696297/1‑94 (MQ=60)
         TCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCAT                                                                                    <  SRR4302537.2608251/94‑1 (MQ=60)
         TCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCAT                                                                                    <  SRR4302537.3269242/94‑1 (MQ=60)
         TCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCAT                                                                                    <  SRR4302537.307101/94‑1 (MQ=60)
          CTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATG                                                                                   >  SRR4302537.5092420/1‑94 (MQ=60)
          CTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATG                                                                                   >  SRR4302537.4984594/1‑94 (MQ=60)
          CTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATG                                                                                   <  SRR4302537.1818751/94‑1 (MQ=60)
          CTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATG                                                                                   >  SRR4302537.5557957/1‑94 (MQ=60)
          CTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATG                                                                                   >  SRR4302537.895627/1‑94 (MQ=60)
           TGGTTGATGAAGATCAGCAGCGTGTGGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGT                                                                                  <  SRR4302537.5790491/94‑1 (MQ=60)
            GGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTG                                                                                 >  SRR4302537.6335225/1‑94 (MQ=60)
             GTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGA                                                                                >  SRR4302537.4846906/1‑94 (MQ=60)
             GTTGATGAAGATCAGCAGCGTGTCGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGA                                                                                <  SRR4302537.5333716/94‑1 (MQ=60)
              TTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAG                                                                               <  SRR4302537.512236/94‑1 (MQ=60)
               TGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGA                                                                              >  SRR4302537.1419987/1‑94 (MQ=60)
                GATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAG                                                                             <  SRR4302537.5738936/94‑1 (MQ=60)
                 ATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTTAGAGT                                                                            >  SRR4302537.2227740/1‑94 (MQ=60)
                 ATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGT                                                                            >  SRR4302537.4678708/1‑94 (MQ=60)
                  TGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTC                                                                           <  SRR4302537.3029703/94‑1 (MQ=60)
                  TGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTC                                                                           >  SRR4302537.4934055/1‑94 (MQ=60)
                   GAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCG                                                                          <  SRR4302537.1555830/94‑1 (MQ=60)
                    AAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGC                                                                         >  SRR4302537.1699612/1‑94 (MQ=60)
                     AGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCC                                                                        >  SRR4302537.5051344/1‑94 (MQ=60)
                     AGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCC                                                                        <  SRR4302537.737860/94‑1 (MQ=60)
                      GATCACNAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCG                                                                       <  SRR4302537.249026/94‑1 (MQ=60)
                        TCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCTCATGTGAGAGTCGCCGAT                                                                     >  SRR4302537.5379441/1‑94 (MQ=60)
                        TCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGAT                                                                     <  SRR4302537.4849032/94‑1 (MQ=60)
                         CAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATT                                                                    <  SRR4302537.1953208/94‑1 (MQ=60)
                          AGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTT                                                                   >  SRR4302537.6141048/1‑94 (MQ=60)
                           ACAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTC                                                                  <  SRR4302537.1313662/94‑1 (MQ=60)
                            CAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCG                                                                 >  SRR4302537.6261187/1‑94 (MQ=60)
                            CAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCG                                                                 >  SRR4302537.1604654/1‑94 (MQ=60)
                            CAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCG                                                                 >  SRR4302537.3633125/1‑94 (MQ=60)
                            CAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCC                                                                 >  SRR4302537.3047932/1‑94 (MQ=60)
                            CAGCGTGTTGGACTGCTTCAGGTAACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCG                                                                 <  SRR4302537.3719624/94‑1 (MQ=60)
                               CGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCT                                                              >  SRR4302537.5248681/1‑94 (MQ=60)
                               CGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCT                                                              <  SRR4302537.488633/94‑1 (MQ=60)
                                gttttggcctctTTCAGGTTCCCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTT                                                             <  SRR4302537.1528366/82‑1 (MQ=60)
                                GTGTTGGCCTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTT                                                             <  SRR4302537.15380/94‑1 (MQ=60)
                                GTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTT                                                             >  SRR4302537.1308291/1‑94 (MQ=60)
                                 TGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTC                                                            >  SRR4302537.1939002/1‑94 (MQ=60)
                                    TGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGAT                                                         <  SRR4302537.6438920/94‑1 (MQ=60)
                                     GGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATT                                                        <  SRR4302537.443936/94‑1 (MQ=60)
                                      GACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTT                                                       >  SRR4302537.5623240/1‑94 (MQ=60)
                                        CTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCC                                                     <  SRR4302537.3339960/94‑1 (MQ=60)
                                        CTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCC                                                     >  SRR4302537.6234528/1‑94 (MQ=60)
                                         TGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGcggcggattcggcttcgcttttcgc                                                    >  SRR4302537.2314111/1‑69 (MQ=60)
                                         TGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCG                                                    <  SRR4302537.4709501/94‑1 (MQ=60)
                                          GCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGC                                                   >  SRR4302537.3924361/1‑94 (MQ=60)
                                           CTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCT                                                  <  SRR4302537.4994505/94‑1 (MQ=60)
                                           CTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCT                                                  <  SRR4302537.1524218/94‑1 (MQ=60)
                                            TTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTT                                                 >  SRR4302537.2836225/1‑94 (MQ=60)
                                            TTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTT                                                 >  SRR4302537.2545393/1‑94 (MQ=60)
                                            TACAGGTTACCCGACTGATTACACATCGCCTGGCTCATCATGCGTGCCACAAGGCCAAAGTGAGAGTCGCCGATTTCGCCATCGATTTCCGCTT                                                 <  SRR4302537.6511725/94‑1 (MQ=48)
                                              CAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTC                                               <  SRR4302537.961262/94‑1 (MQ=60)
                                               AGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCG                                              >  SRR4302537.5911451/1‑94 (MQ=60)
                                               AGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTCCCACAAGGCCCCTGTGAGAGTCGCCGCTTTAGCCTTCGATTTACGCTTTCG                                              >  SRR4302537.3944257/1‑94 (MQ=60)
                                                GGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGG                                             >  SRR4302537.2966354/1‑94 (MQ=60)
                                                GGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGG                                             >  SRR4302537.2966495/1‑94 (MQ=60)
                                                GGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGG                                             >  SRR4302537.4847885/1‑94 (MQ=60)
                                                GGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGG                                             >  SRR4302537.247232/1‑94 (MQ=60)
                                                GGGTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGG                                             <  SRR4302537.1662757/94‑1 (MQ=60)
                                                 GTTCCCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGC                                            <  SRR4302537.5978372/94‑1 (MQ=60)
                                                 GTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGC                                            <  SRR4302537.4450084/94‑1 (MQ=60)
                                                 GTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTACGCTTTCGGG                                            >  SRR4302537.2136038/1‑94 (MQ=60)
                                                  TTCCCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCG                                           <  SRR4302537.3356158/94‑1 (MQ=60)
                                                   TACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGT                                          <  SRR4302537.4999067/94‑1 (MQ=60)
                                                   TACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGT                                          <  SRR4302537.4914117/94‑1 (MQ=60)
                                                    ACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTC                                         >  SRR4302537.6445947/1‑94 (MQ=60)
                                                    ACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTC                                         >  SRR4302537.5106636/1‑94 (MQ=60)
                                                     CCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCA                                        >  SRR4302537.1234914/1‑94 (MQ=60)
                                                     CCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCA                                        >  SRR4302537.2770221/1‑94 (MQ=60)
                                                     CCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCA                                        >  SRR4302537.3671128/1‑94 (MQ=60)
                                                     CCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCA                                        >  SRR4302537.3645369/1‑94 (MQ=60)
                                                      CCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAG                                       >  SRR4302537.4176621/1‑94 (MQ=60)
                                                      CCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAG                                       >  SRR4302537.5830692/1‑94 (MQ=60)
                                                       CGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGTCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGT                                      <  SRR4302537.4077403/94‑1 (MQ=60)
                                                       CGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGT                                      >  SRR4302537.2500031/1‑94 (MQ=60)
                                                        GCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTG                                     >  SRR4302537.1896021/1‑94 (MQ=60)
                                                        GCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTG                                     <  SRR4302537.5822528/94‑1 (MQ=60)
                                                        GCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTAGCCTTCGATTTGCGCTTTCGGCGTCAGTG                                     >  SRR4302537.539282/1‑94 (MQ=60)
                                                         CCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGC                                    <  SRR4302537.4246964/94‑1 (MQ=60)
                                                         CCAGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGC                                    <  SRR4302537.293186/94‑1 (MQ=60)
                                                          CAGCTTACGCATCAGCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCAGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCC                                   <  SRR4302537.2054815/94‑1 (MQ=60)
                                                           AGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCG                                  <  SRR4302537.2978634/94‑1 (MQ=60)
                                                           AGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCG                                  >  SRR4302537.2450171/1‑94 (MQ=60)
                                                           AGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCG                                  <  SRR4302537.913394/94‑1 (MQ=60)
                                                           AGCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCG                                  <  SRR4302537.4214709/94‑1 (MQ=60)
                                                            GCTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGC                                 >  SRR4302537.290281/1‑94 (MQ=60)
                                                             CTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCC                                >  SRR4302537.5615580/1‑94 (MQ=60)
                                                             CTTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCC                                >  SRR4302537.433460/1‑94 (MQ=60)
                                                              TTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCA                               >  SRR4302537.1092424/1‑94 (MQ=60)
                                                              TTACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCA                               >  SRR4302537.5203238/1‑94 (MQ=60)
                                                               TACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCAC                              <  SRR4302537.4818893/94‑1 (MQ=60)
                                                                ACGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACG                             >  SRR4302537.176522/1‑94 (MQ=60)
                                                                 CGCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGG                            >  SRR4302537.5534070/1‑94 (MQ=60)
                                                                  GCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGA                           <  SRR4302537.1963432/94‑1 (MQ=60)
                                                                  GCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGA                           >  SRR4302537.5694650/1‑94 (MQ=60)
                                                                  GCATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGA                           >  SRR4302537.5867477/1‑94 (MQ=60)
                                                                   CATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAG                          >  SRR4302537.5979545/1‑94 (MQ=60)
                                                                   CATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGGCGCCGATTTAGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGCG                          >  SRR4302537.1516425/1‑94 (MQ=60)
                                                                    ATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGT                         >  SRR4302537.1228757/1‑94 (MQ=60)
                                                                    ATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGT                         >  SRR4302537.4088862/1‑94 (MQ=60)
                                                                    ATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGT                         >  SRR4302537.5974459/1‑94 (MQ=60)
                                                                    ATCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCCGCGTCAGTGCCGCCACGGAGG                         >  SRR4302537.335742/1‑94 (MQ=60)
                                                                     TCGCNTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTC                        <  SRR4302537.2163101/94‑1 (MQ=60)
                                                                     TCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTC                        <  SRR4302537.1503041/94‑1 (MQ=60)
                                                                     TCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTC                        <  SRR4302537.3962/94‑1 (MQ=60)
                                                                     TCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTC                        <  SRR4302537.2914549/94‑1 (MQ=60)
                                                                     TCGCCTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTC                        <  SRR4302537.4431497/94‑1 (MQ=60)
                                                                      CGCCTTGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTGCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCA                       <  SRR4302537.2114026/94‑1 (MQ=60)
                                                                         CTGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACG                    >  SRR4302537.2644065/1‑94 (MQ=60)
                                                                          TGGCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGA                   >  SRR4302537.1759139/1‑94 (MQ=60)
                                                                            GCTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACG                 <  SRR4302537.1305155/94‑1 (MQ=60)
                                                                             CTCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGA                >  SRR4302537.1781146/1‑94 (MQ=60)
                                                                              TCATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGAT               >  SRR4302537.5079755/1‑94 (MQ=60)
                                                                               CATCATACGTGCCGCAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATA              >  SRR4302537.2112872/1‑94 (MQ=60)
                                                                               CATCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATA              >  SRR4302537.3810108/1‑94 (MQ=60)
                                                                                 TCATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAAC            <  SRR4302537.2544961/94‑1 (MQ=60)
                                                                                  CATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACG           >  SRR4302537.6240730/1‑94 (MQ=60)
                                                                                  CATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACG           >  SRR4302537.899037/1‑94 (MQ=60)
                                                                                   ATACGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGT          >  SRR4302537.662369/1‑94 (MQ=60)
                                                                                      CGTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTA       >  SRR4302537.3815509/1‑94 (MQ=60)
                                                                                       GTGCCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTAC      <  SRR4302537.3239259/94‑1 (MQ=60)
                                                                                          CCACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGC   >  SRR4302537.4253068/1‑94 (MQ=60)
                                                                                           CACAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCG  <  SRR4302537.4414423/94‑1 (MQ=60)
                                                                                            |                                                                                            
TCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCG  >  NC_000913/2821198‑2821382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑