Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,882,127 C→T R12H (CGC→CAC)  b2760 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,882,1270CT94.1% 778.1 / 21.5 239R12H (CGC→CAC) b2760hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (6/8);  new base T (112/113);  total (118/121)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.47e-02

Only 100 of 248 total aligned reads displayed.

TCGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCACACTT  >  NC_000913/2882037‑2882218
                                                                                          |                                                                                           
tCGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGgtgt                                                                                           >  1:4174013/1‑93 (MQ=255)
tCGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGgtgt                                                                                           <  2:466780/93‑1 (MQ=255)
 cGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTa                                                                                          >  2:4685148/1‑93 (MQ=255)
    cACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAg                                                                                       <  2:652709/93‑1 (MQ=255)
     aCGGGGCAAACTTAATCGCCACTGAGCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAgg                                                                                      <  1:5828371/93‑1 (MQ=255)
      cGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAggg                                                                                     <  1:3849693/93‑1 (MQ=255)
       ggggCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGa                                                                                    <  1:1328500/93‑1 (MQ=255)
         ggCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATc                                                                                  >  2:1306818/1‑93 (MQ=255)
          gCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATcc                                                                                 <  2:2415619/93‑1 (MQ=255)
           cAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCa                                                                                <  1:3309991/93‑1 (MQ=255)
           cAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCa                                                                                >  1:2917953/1‑93 (MQ=255)
            aaaCTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAg                                                                               >  2:260940/1‑93 (MQ=255)
            aaaCTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAg                                                                               <  1:1649789/93‑1 (MQ=255)
            aaaCTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAg                                                                               >  2:4846212/1‑93 (MQ=255)
             aaCTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGt                                                                              <  1:1076811/93‑1 (MQ=255)
             aaCTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGt                                                                              >  2:3526901/1‑93 (MQ=255)
              aCTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGtt                                                                             >  1:6409349/1‑93 (MQ=255)
               cTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTa                                                                            <  1:4270163/93‑1 (MQ=255)
                ttAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTCCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTAt                                                                           <  1:2701247/93‑1 (MQ=255)
                 tAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCCGATTTATGATTTGGACTTTCGCCCCGTTTCGCGGCTGTACAGGGATCCAGTCATc                                                                          >  2:6376579/1‑93 (MQ=255)
                 tAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATc                                                                          <  1:3516323/93‑1 (MQ=255)
                  aaTCGCCACTGATCTCTACCTCAATATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCa                                                                         <  1:1255257/93‑1 (MQ=255)
                   aTCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCaa                                                                        >  2:3573340/1‑93 (MQ=255)
                   aTCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCaa                                                                        >  2:3330690/1‑93 (MQ=255)
                   aTCGCCAATGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCaa                                                                        >  2:6142537/1‑93 (MQ=255)
                        cACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGc                                                                   >  2:5611691/1‑93 (MQ=255)
                         aCTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCa                                                                  <  2:4357845/93‑1 (MQ=255)
                           tGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCaaa                                                                <  2:3960226/93‑1 (MQ=255)
                            gATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAAt                                                               >  1:6354218/1‑93 (MQ=255)
                            gATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAAt                                                               <  2:4502872/93‑1 (MQ=255)
                            gATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAAt                                                               <  1:4273810/93‑1 (MQ=255)
                            gATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAAt                                                               >  1:4626717/1‑93 (MQ=255)
                             aTCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAAtt                                                              >  2:2341791/1‑93 (MQ=255)
                              tctcTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTc                                                             >  1:2714832/1‑93 (MQ=255)
                              tctcTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATACAGTTATCAATAAGCAAATTc                                                             >  1:3222099/1‑93 (MQ=255)
                               ctctACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCa                                                            <  1:2511908/93‑1 (MQ=255)
                                tctACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCAt                                                           <  2:2862013/93‑1 (MQ=255)
                                 ctACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCAtt                                                          <  1:1756685/93‑1 (MQ=255)
                                 ctACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCAtt                                                          >  2:5717957/1‑93 (MQ=255)
                                  tACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCAttt                                                         >  2:5280399/1‑93 (MQ=255)
                                  tACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCAttt                                                         <  2:2525160/93‑1 (MQ=255)
                                  tACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCAttt                                                         >  2:2979143/1‑93 (MQ=255)
                                    cTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGt                                                       >  1:15215/1‑93 (MQ=255)
                                    cTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGt                                                       >  1:4688891/1‑93 (MQ=255)
                                      gCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTATAGGGGGCCAGTTATCAATAAGCAAATTCATTTGTtc                                                     >  1:6114682/1‑93 (MQ=255)
                                      gCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTtc                                                     >  2:3943250/1‑93 (MQ=255)
                                      gCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTtc                                                     >  1:6260667/1‑93 (MQ=255)
                                      gCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTtc                                                     >  1:5502161/1‑93 (MQ=255)
                                      gCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTtc                                                     >  1:2841/1‑93 (MQ=255)
                                       cAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTtct                                                    <  1:1405731/93‑1 (MQ=255)
                                        aGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCACTTATCAATAAGCAAATTCATTTGTtctc                                                   >  1:4542195/1‑93 (MQ=255)
                                         gTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTcc                                                  <  1:5343356/93‑1 (MQ=255)
                                         gTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTcc                                                  <  1:851475/93‑1 (MQ=255)
                                         gTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTcc                                                  >  2:108334/1‑93 (MQ=255)
                                          tagaGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCt                                                 <  2:2705635/90‑1 (MQ=255)
                                           ataGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCtt                                                >  1:1554205/1‑93 (MQ=255)
                                           ataGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCtt                                                >  2:2161736/1‑93 (MQ=255)
                                            taGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTc                                               <  1:5003143/93‑1 (MQ=255)
                                            taGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTc                                               <  1:4524810/93‑1 (MQ=255)
                                            taGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTc                                               >  2:3188954/1‑93 (MQ=255)
                                              gCGATTGCAGATTTATGATTTGGACTTCCCTCTCGTTTCGCGGGCGTACAGGGATCCAGATATCAGTAACCAAGTTCATTTGTTCTCCTTCat                                             <  2:4268246/93‑1 (MQ=255)
                                               cGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCata                                            <  2:1847907/93‑1 (MQ=255)
                                               cGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCata                                            <  2:2924217/93‑1 (MQ=255)
                                                gATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCatat                                           >  1:2197072/1‑93 (MQ=255)
                                                gATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCatat                                           >  2:5967/1‑93 (MQ=255)
                                                gATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCatat                                           >  2:802948/1‑93 (MQ=255)
                                                 aTTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATg                                          <  2:1491064/93‑1 (MQ=255)
                                                  ttGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGc                                         >  1:3697564/1‑93 (MQ=255)
                                                    gCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTc                                       <  2:1428314/93‑1 (MQ=255)
                                                     cAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTcc                                      <  2:5552016/93‑1 (MQ=255)
                                                      aGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCg                                     >  2:2218288/1‑93 (MQ=255)
                                                       gATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGa                                    >  1:1969039/1‑93 (MQ=255)
                                                          ttATGATTTGGACTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACAt                                 <  1:1153916/93‑1 (MQ=255)
                                                           tATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACAtt                                >  1:1007345/1‑93 (MQ=255)
                                                           tATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACAtt                                >  1:5269662/1‑93 (MQ=255)
                                                           tATGATTTAGACTTTCCCCTCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACAtt                                >  2:6270025/1‑93 (MQ=255)
                                                             tGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACAtttg                              >  2:5066951/1‑92 (MQ=255)
                                                              gATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCAAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTtct                             >  2:2059255/1‑93 (MQ=255)
                                                                tttGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTcc                           >  2:5955539/1‑93 (MQ=255)
                                                                     aCTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCAt                      <  1:180871/93‑1 (MQ=255)
                                                                      cTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCAtt                     <  1:5021258/93‑1 (MQ=255)
                                                                      cTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCAtt                     <  1:5563528/93‑1 (MQ=255)
                                                                        ttCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTc                   <  2:747458/93‑1 (MQ=255)
                                                                        ttCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTc                   <  1:5860370/93‑1 (MQ=255)
                                                                           cccccGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCtat                >  1:1290789/1‑93 (MQ=255)
                                                                           cccccGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCtat                >  1:76233/1‑93 (MQ=255)
                                                                           cccccGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATCTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCtat                <  1:3465458/93‑1 (MQ=255)
                                                                            ccccGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCtata               <  1:1632793/93‑1 (MQ=255)
                                                                            ccccGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCtata               >  2:5821720/1‑93 (MQ=255)
                                                                               cGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGt            <  1:2262711/93‑1 (MQ=255)
                                                                                gTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTc           <  2:3846911/93‑1 (MQ=255)
                                                                                gTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTc           >  1:515337/1‑93 (MQ=255)
                                                                                gTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTc           <  1:4145919/93‑1 (MQ=255)
                                                                                gTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTc           >  1:2069065/1‑93 (MQ=255)
                                                                                 tttCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCg          <  1:2422121/93‑1 (MQ=255)
                                                                                  ttCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCgg         >  1:6032398/1‑93 (MQ=255)
                                                                                     gcgGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGcac      >  2:5305072/1‑93 (MQ=255)
                                                                                      cgGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGcaca     <  1:3138204/93‑1 (MQ=255)
                                                                                       gggTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCacac    <  1:4786596/93‑1 (MQ=255)
                                                                                         gCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCACACtt  <  2:4240138/93‑1 (MQ=255)
                                                                                          |                                                                                           
TCGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCACACTT  >  NC_000913/2882037‑2882218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATCGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCACACTTCC  >  NC_000913/2882034‑2882220
                                                                                             |                                                                                             
ATATCGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGT                                                                                               >  SRR4302537.4160823/1‑94 (MQ=60)
ATATCGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGG                                                                                               >  SRR4302537.478142/1‑94 (MQ=60)
  ATCGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGT                                                                                             <  SRR4302537.5237964/94‑1 (MQ=60)
  ATCGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGT                                                                                             >  SRR4302537.4203159/1‑94 (MQ=60)
    CGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTAC                                                                                           <  SRR4302537.997831/94‑1 (MQ=60)
    CGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTAC                                                                                           >  SRR4302537.4672076/1‑94 (MQ=60)
    CGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTAC                                                                                           >  SRR4302537.2151095/1‑94 (MQ=60)
     GTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACA                                                                                          >  SRR4302537.5420446/1‑94 (MQ=60)
     GTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACA                                                                                          >  SRR4302537.1113302/1‑94 (MQ=60)
     GTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACA                                                                                          <  SRR4302537.36807/94‑1 (MQ=60)
     GTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTATACA                                                                                          >  SRR4302537.33163/1‑94 (MQ=60)
      TCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAG                                                                                         <  SRR4302537.2303137/94‑1 (MQ=60)
       CACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGG                                                                                        <  SRR4302537.3699954/94‑1 (MQ=60)
        ACGGGGCAAACTTAATCGCCACTGAGCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGG                                                                                       <  SRR4302537.5868318/94‑1 (MQ=60)
         CGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGA                                                                                      <  SRR4302537.3876653/94‑1 (MQ=60)
         CGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGA                                                                                      <  SRR4302537.1732739/94‑1 (MQ=60)
          GGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGAT                                                                                     <  SRR4302537.1337762/94‑1 (MQ=60)
           GGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATC                                                                                    >  SRR4302537.477709/1‑94 (MQ=60)
           GGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATC                                                                                    <  SRR4302537.60146/94‑1 (MQ=60)
           GGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATC                                                                                    >  SRR4302537.4598658/1‑94 (MQ=60)
             GCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCA                                                                                  >  SRR4302537.2938455/1‑94 (MQ=60)
             GCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCA                                                                                  >  SRR4302537.2569083/1‑94 (MQ=60)
              CAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAG                                                                                 <  SRR4302537.1302808/94‑1 (MQ=60)
              CAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAG                                                                                 <  SRR4302537.3333405/94‑1 (MQ=60)
               AAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGT                                                                                >  SRR4302537.2351747/1‑94 (MQ=60)
               AAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGT                                                                                >  SRR4302537.1852903/1‑94 (MQ=60)
               AAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGT                                                                                <  SRR4302537.1661365/94‑1 (MQ=60)
               AAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGT                                                                                >  SRR4302537.2498448/1‑94 (MQ=60)
                AACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTT                                                                               <  SRR4302537.1084229/94‑1 (MQ=60)
                AACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTT                                                                               >  SRR4302537.6452960/1‑94 (MQ=60)
                  CTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTAT                                                                             >  SRR4302537.4468821/1‑94 (MQ=60)
                  CTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTAT                                                                             <  SRR4302537.4299830/94‑1 (MQ=60)
                   TTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATC                                                                            >  SRR4302537.5739038/1‑94 (MQ=60)
                   TTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTCCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATC                                                                            <  SRR4302537.2720111/94‑1 (MQ=60)
                    TAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCA                                                                           <  SRR4302537.3541201/94‑1 (MQ=60)
                     AATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAA                                                                          <  SRR4302537.4853105/94‑1 (MQ=60)
                     AATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAA                                                                          >  SRR4302537.952842/1‑94 (MQ=60)
                     AATCGCCACTGATCTCTACCTCAATATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAA                                                                          <  SRR4302537.1263990/94‑1 (MQ=60)
                      ATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAAT                                                                         >  SRR4302537.3056541/1‑94 (MQ=60)
                         GCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAG                                                                      >  SRR4302537.6098323/1‑94 (MQ=60)
                          CCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGC                                                                     <  SRR4302537.5730176/94‑1 (MQ=60)
                           CACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCA                                                                    >  SRR4302537.5222586/1‑94 (MQ=60)
                             CTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAA                                                                  >  SRR4302537.5043084/1‑94 (MQ=60)
                              TGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAAT                                                                 <  SRR4302537.3311076/94‑1 (MQ=60)
                              TGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAAT                                                                 >  SRR4302537.6397590/1‑94 (MQ=60)
                              TGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAAT                                                                 >  SRR4302537.3086649/1‑94 (MQ=60)
                              TGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAAT                                                                 >  SRR4302537.4658736/1‑94 (MQ=60)
                              TGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAAT                                                                 >  SRR4302537.6451646/1‑94 (MQ=60)
                               GATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATT                                                                <  SRR4302537.6487311/94‑1 (MQ=60)
                               GATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATT                                                                <  SRR4302537.4031788/94‑1 (MQ=60)
                               GATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATT                                                                <  SRR4302537.4303510/94‑1 (MQ=60)
                                ATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTC                                                               >  SRR4302537.2733789/1‑94 (MQ=60)
                                ATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATACAGTTATCAATAAGCAAATTC                                                               >  SRR4302537.3244773/1‑94 (MQ=60)
                                 TCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCA                                                              <  SRR4302537.510473/94‑1 (MQ=60)
                                 TCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCA                                                              >  SRR4302537.5910881/1‑94 (MQ=60)
                                  CTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCAT                                                             >  SRR4302537.2574764/1‑94 (MQ=60)
                                  CTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCAT                                                             <  SRR4302537.2529571/94‑1 (MQ=60)
                                    CTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTT                                                           <  SRR4302537.1768929/94‑1 (MQ=60)
                                    CTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTT                                                           <  SRR4302537.1309469/94‑1 (MQ=60)
                                     TACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTG                                                          <  SRR4302537.1803490/94‑1 (MQ=60)
                                      ACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGT                                                         <  SRR4302537.1352877/94‑1 (MQ=60)
                                      ACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGT                                                         >  SRR4302537.15336/1‑94 (MQ=60)
                                      ACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGT                                                         >  SRR4302537.1543065/1‑94 (MQ=60)
                                      ACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGT                                                         >  SRR4302537.4721326/1‑94 (MQ=60)
                                       CTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTT                                                        <  SRR4302537.4356953/94‑1 (MQ=60)
                                       CTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTT                                                        >  SRR4302537.6216057/1‑94 (MQ=60)
                                       CTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTT                                                        >  SRR4302537.1605909/1‑94 (MQ=60)
                                       CTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTT                                                        >  SRR4302537.4251477/1‑94 (MQ=60)
                                        TGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCGCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTC                                                       <  SRR4302537.5437000/94‑1 (MQ=60)
                                        TGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTATAGGGGGCCAGTTATCAATAAGCAAATTCATTTGTTC                                                       >  SRR4302537.6156699/1‑94 (MQ=60)
                                        TGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTC                                                       >  SRR4302537.5539731/1‑94 (MQ=60)
                                        TGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTC                                                       >  SRR4302537.1234755/1‑94 (MQ=60)
                                        TGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTC                                                       >  SRR4302537.6303651/1‑94 (MQ=60)
                                        TGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTC                                                       >  SRR4302537.2862/1‑94 (MQ=60)
                                          CAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTC                                                     <  SRR4302537.1415520/94‑1 (MQ=60)
                                          CAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCACTTATCAATAAGCAAATTCATTTGTTCTC                                                     >  SRR4302537.4573634/1‑94 (MQ=60)
                                           AGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCC                                                    <  SRR4302537.4567710/94‑1 (MQ=60)
                                            GTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCT                                                   <  SRR4302537.6348428/94‑1 (MQ=60)
                                            GTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCT                                                   <  SRR4302537.857310/94‑1 (MQ=60)
                                            GTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCT                                                   <  SRR4302537.2145599/94‑1 (MQ=60)
                                            GTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCT                                                   <  SRR4302537.5379974/94‑1 (MQ=60)
                                            GTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCT                                                   <  SRR4302537.4764217/94‑1 (MQ=60)
                                             TATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTT                                                  >  SRR4302537.1565048/1‑94 (MQ=60)
                                              ATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTC                                                 <  SRR4302537.1714787/94‑1 (MQ=60)
                                              ATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTC                                                 >  SRR4302537.3864455/1‑94 (MQ=60)
                                               TAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCA                                                <  SRR4302537.4556117/94‑1 (MQ=60)
                                               TAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCA                                                <  SRR4302537.5037604/94‑1 (MQ=60)
                                                AGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCAT                                               <  SRR4302537.5321133/94‑1 (MQ=60)
                                                AGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCAT                                               <  SRR4302537.4619017/94‑1 (MQ=60)
                                                  CGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATAT                                             >  SRR4302537.129985/1‑94 (MQ=60)
                                                  CGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATAT                                             <  SRR4302537.353488/94‑1 (MQ=60)
                                                  CGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATAT                                             <  SRR4302537.3164288/94‑1 (MQ=60)
                                                  CGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATAT                                             >  SRR4302537.2212552/1‑94 (MQ=60)
                                                   GATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATG                                            <  SRR4302537.4255334/94‑1 (MQ=60)
                                                   GATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATG                                            >  SRR4302537.6238973/1‑94 (MQ=60)
                                                   GATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATG                                            >  SRR4302537.160710/1‑94 (MQ=60)
                                                    ATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGC                                           >  SRR4302537.3723581/1‑94 (MQ=60)
                                                        CAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCG                                       <  SRR4302537.1450845/94‑1 (MQ=60)
                                                         AGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGA                                      >  SRR4302537.1982733/1‑94 (MQ=60)
                                                          GATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGAC                                     >  SRR4302537.1865615/1‑94 (MQ=60)
                                                           ATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACA                                    >  SRR4302537.3413233/1‑94 (MQ=60)
                                                            TTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACAT                                   <  SRR4302537.5244121/94‑1 (MQ=60)
                                                            TTTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAACCAAATTAATTTGTTCTCCTTCATATGttccgcaat                                   >  SRR4302537.2799652/1‑85 (MQ=60)
                                                             TTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATT                                  >  SRR4302537.5305926/1‑94 (MQ=60)
                                                             TTATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATT                                  >  SRR4302537.1014298/1‑94 (MQ=60)
                                                             TTATGATTTGGACTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATT                                  <  SRR4302537.1161946/94‑1 (MQ=60)
                                                               ATGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTC                                <  SRR4302537.1474911/94‑1 (MQ=60)
                                                                TGATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCT                               <  SRR4302537.1186376/94‑1 (MQ=60)
                                                                  ATTTGGACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCC                             >  SRR4302537.164564/1‑94 (MQ=60)
                                                                   TTTGCACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTCCATATGCTCCGACATTTCTCCT                            <  SRR4302537.4300179/94‑1 (MQ=60)
                                                                       GACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCAT                        >  SRR4302537.5044827/1‑94 (MQ=60)
                                                                        ACTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATT                       <  SRR4302537.182157/94‑1 (MQ=60)
                                                                         CTTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTT                      <  SRR4302537.5055841/94‑1 (MQ=60)
                                                                         CTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTT                      <  SRR4302537.5601553/94‑1 (MQ=60)
                                                                          TTTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTC                     <  SRR4302537.6460046/94‑1 (MQ=60)
                                                                           TTCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCT                    <  SRR4302537.5900556/94‑1 (MQ=60)
                                                                            TCCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTA                   >  SRR4302537.3936497/1‑94 (MQ=60)
                                                                             CCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTAT                  >  SRR4302537.2900156/1‑94 (MQ=60)
                                                                             CCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTAT                  >  SRR4302537.76794/1‑94 (MQ=60)
                                                                             CCCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTAT                  >  SRR4302537.1299766/1‑94 (MQ=60)
                                                                              CCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATA                 >  SRR4302537.799957/1‑94 (MQ=60)
                                                                              CCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATA                 <  SRR4302537.104281/94‑1 (MQ=60)
                                                                              CCCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATCTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATA                 <  SRR4302537.3489963/94‑1 (MQ=60)
                                                                               CCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATAC                >  SRR4302537.2248633/1‑94 (MQ=60)
                                                                               CCCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATAC                <  SRR4302537.1644233/94‑1 (MQ=60)
                                                                                CCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACT               >  SRR4302537.4178432/1‑94 (MQ=60)
                                                                                CCCGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACG               >  SRR4302537.199576/1‑94 (MQ=60)
                                                                                  CGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTC             <  SRR4302537.2278677/94‑1 (MQ=60)
                                                                                  CGTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTC             >  SRR4302537.518882/1‑94 (MQ=60)
                                                                                  CGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTC             >  SRR4302537.2083602/1‑94 (MQ=60)
                                                                                   GTTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCG            <  SRR4302537.4174847/94‑1 (MQ=60)
                                                                                    TTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGG           >  SRR4302537.6073848/1‑94 (MQ=60)
                                                                                    TTTCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGG           <  SRR4302537.2439184/94‑1 (MQ=60)
                                                                                      TCGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCA         <  SRR4302537.5871096/94‑1 (MQ=60)
                                                                                       CGCGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCAC        <  SRR4302537.282045/94‑1 (MQ=60)
                                                                                         CGGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCACAC      <  SRR4302537.3160225/94‑1 (MQ=60)
                                                                                          GGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCACACT     <  SRR4302537.2721893/94‑1 (MQ=60)
                                                                                          GGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCACACT     >  SRR4302537.1484558/1‑94 (MQ=60)
                                                                                          GGGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCACACT     <  SRR4302537.4819631/94‑1 (MQ=60)
                                                                                           GGTGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCACACTT    >  SRR4302537.3357984/1‑94 (MQ=60)
                                                                                             TGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCACACTTCC  <  SRR4302537.2157510/94‑1 (MQ=60)
                                                                                             TGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCACACTTCC  <  SRR4302537.3931329/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATATCGTCACGGGGCAAACTTAATCGCCACTGATCTCTACTGCAGTATAGCGATTGCAGATTTATGATTTGGACTTTCCCCCCGTTTCGCGGGCGTACAGGGATCCAGTTATCAATAAGCAAATTCATTTGTTCTCCTTCATATGCTCCGACATTTCTCCTGCATTTCTATACGTCGGCACACTTCC  >  NC_000913/2882034‑2882220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑