Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,899,155 A→G Y181C (TAC→TGC)  yqcE → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,899,1550AG97.3% 865.3 / 7.4 259Y181C (TAC→TGC) yqcEputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (4/3);  new base G (121/131);  total (125/134)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 262 total aligned reads displayed.

TGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGC  >  NC_000913/2899065‑2899244
                                                                                          |                                                                                         
tGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCa                                                                                         >  2:5310015/1‑93 (MQ=255)
tGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCa                                                                                         >  2:5066886/1‑93 (MQ=255)
 ggCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCAt                                                                                        >  2:2098675/1‑93 (MQ=255)
 ggCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCAt                                                                                        >  1:4185833/1‑93 (MQ=255)
  gCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATc                                                                                       <  1:4009983/93‑1 (MQ=255)
  gCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATc                                                                                       <  1:1686441/93‑1 (MQ=255)
  gCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATc                                                                                       <  2:4387963/93‑1 (MQ=255)
   cGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCt                                                                                      <  1:427057/93‑1 (MQ=255)
    ggTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATTACAGCACCAGCCTGAAAACGGGCATTATCATCTATAGTGTGGTTTGCATCtt                                                                                     >  2:3356139/1‑93 (MQ=255)
     gtgtTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCttg                                                                                    <  1:4619678/93‑1 (MQ=255)
      tgtTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCttgt                                                                                   >  2:2158267/1‑93 (MQ=255)
      tgtTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCttgt                                                                                   <  2:3247887/93‑1 (MQ=255)
        tttACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCttgttg                                                                                 >  1:1005197/1‑93 (MQ=255)
        tttACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCttgttg                                                                                 <  2:5543888/93‑1 (MQ=255)
          tACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTggg                                                                               <  2:2813212/93‑1 (MQ=255)
           aCCATGTGGGTCTTTTCTCGCTTTGCACTGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTgggg                                                                              <  1:2695408/93‑1 (MQ=255)
           aCCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTgggg                                                                              >  1:5826952/1‑93 (MQ=255)
            ccATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGa                                                                             >  1:1257995/1‑93 (MQ=255)
             cATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGAt                                                                            >  1:5258133/1‑93 (MQ=255)
             cATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGAt                                                                            >  1:3522697/1‑93 (MQ=255)
             cATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGAt                                                                            <  1:3556401/93‑1 (MQ=255)
             cATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGAt                                                                            >  1:296325/1‑93 (MQ=255)
              aTGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGAtt                                                                           >  2:3427507/1‑93 (MQ=255)
              aTGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGAtt                                                                           <  1:1653430/93‑1 (MQ=255)
              aTGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGAtt                                                                           <  2:4596766/93‑1 (MQ=255)
               tgtgGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTc                                                                          <  2:2856/93‑1 (MQ=255)
               tgtgGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTc                                                                          >  2:5904266/1‑93 (MQ=255)
                 tgGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCtg                                                                        <  2:4030347/93‑1 (MQ=255)
                  gggTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCtgt                                                                       >  2:2899237/1‑93 (MQ=255)
                   ggTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCtgtg                                                                      >  2:1118477/1‑93 (MQ=255)
                   ggTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCtgtg                                                                      <  2:194781/93‑1 (MQ=255)
                    gTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGc                                                                     <  2:3457257/93‑1 (MQ=255)
                     tCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCt                                                                    <  1:1388661/93‑1 (MQ=255)
                       ttttCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTTGGTTGCATCTTGTTTGGGATTCTGTGCTgg                                                                  >  1:2565568/1‑93 (MQ=255)
                        tttCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGt                                                                 <  2:3943733/93‑1 (MQ=255)
                        tttCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGt                                                                 <  2:1616983/93‑1 (MQ=255)
                          tctcGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGttt                                                               <  2:2482899/93‑1 (MQ=255)
                          tctcGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGttt                                                               <  1:4965039/93‑1 (MQ=255)
                          tctcGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGttt                                                               >  2:3812835/1‑93 (MQ=255)
                            tcGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGttttt                                                             <  1:5788433/93‑1 (MQ=255)
                            tcGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGttttt                                                             <  2:4762228/93‑1 (MQ=255)
                            tcGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGttttt                                                             >  1:871599/1‑93 (MQ=255)
                             cGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGtttttt                                                            <  2:4331934/93‑1 (MQ=255)
                             cGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGtttttt                                                            <  1:1221133/93‑1 (MQ=255)
                             cGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGtttttt                                                            <  1:2452353/93‑1 (MQ=255)
                              gCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTg                                                           >  2:442738/1‑93 (MQ=255)
                                tttGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGtt                                                         >  1:5372714/1‑93 (MQ=255)
                                 ttGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTa                                                        <  1:3723430/93‑1 (MQ=255)
                                 ttGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTa                                                        >  1:3086522/1‑93 (MQ=255)
                                 ttGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTa                                                        <  1:1818208/93‑1 (MQ=255)
                                      ccGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGAt                                                   <  1:4465607/93‑1 (MQ=255)
                                       cGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATa                                                  <  1:2481919/93‑1 (MQ=255)
                                        ggATGACAGCACCAGCCTGAATACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATaa                                                 <  1:3180938/93‑1 (MQ=255)
                                        ggATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATaa                                                 <  1:4768585/93‑1 (MQ=255)
                                          aTGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATaaca                                               >  2:2644965/1‑93 (MQ=255)
                                           tGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATaacaa                                              <  1:68508/93‑1 (MQ=255)
                                           tGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATaacaa                                              <  1:4550138/93‑1 (MQ=255)
                                           tGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATaacaa                                              <  2:1591122/93‑1 (MQ=255)
                                           tGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGG‑TTTTTGTTAGCGATaacaac                                             <  2:2711107/93‑1 (MQ=255)
                                             aCAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATaacaaca                                            <  2:6456155/93‑1 (MQ=255)
                                                gCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAAcc                                         <  1:3678556/93‑1 (MQ=255)
                                                   ccAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTgc                                      <  1:5450168/93‑1 (MQ=255)
                                                   ccAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTgc                                      >  2:2271895/1‑93 (MQ=255)
                                                   ccAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTgc                                      <  1:3986410/93‑1 (MQ=255)
                                                     aGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTgcgc                                    <  1:5606911/93‑1 (MQ=255)
                                                     aGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTgcgc                                    >  2:5724193/1‑93 (MQ=255)
                                                      gCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCa                                   <  1:1551690/93‑1 (MQ=255)
                                                       ccTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAg                                  <  2:758915/93‑1 (MQ=255)
                                                         tGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTg                                >  2:6397580/1‑93 (MQ=255)
                                                          gAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGc                               >  2:2419178/1‑93 (MQ=255)
                                                           aaaaCGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGcc                              <  2:6186163/93‑1 (MQ=255)
                                                            aaaCGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACGACCTGCGCAGTGCCa                             <  2:6059986/93‑1 (MQ=255)
                                                            aaaCGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCa                             >  1:6186577/1‑93 (MQ=255)
                                                            aaaCGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCa                             >  2:4789458/1‑93 (MQ=255)
                                                             aaCGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCaa                            >  1:4242319/1‑93 (MQ=255)
                                                                ggTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATaa                         >  1:5975432/1‑93 (MQ=255)
                                                                ggTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATaa                         <  1:345980/93‑1 (MQ=255)
                                                                 gTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAAc                        <  2:3765810/93‑1 (MQ=255)
                                                                 gTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAAc                        >  2:178391/1‑93 (MQ=255)
                                                                   cATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACga                      >  1:5043953/1‑93 (MQ=255)
                                                                    aTTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACgaa                     >  1:2125277/1‑93 (MQ=255)
                                                                      tATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACgaaga                   >  1:3329289/1‑93 (MQ=255)
                                                                         catcTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGaaaa                >  2:1003644/1‑93 (MQ=255)
                                                                         catcTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGaaaa                <  2:3558425/93‑1 (MQ=255)
                                                                         catcTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGaaaa                <  2:2524565/93‑1 (MQ=255)
                                                                          atcTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGaaaaa               <  1:144319/93‑1 (MQ=255)
                                                                          atcTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGaaaaa               <  2:4115464/93‑1 (MQ=255)
                                                                             tataGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAg            >  1:19637/1‑93 (MQ=255)
                                                                             tataGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAg            <  1:2279032/93‑1 (MQ=255)
                                                                             tataGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAg            <  1:1311927/93‑1 (MQ=255)
                                                                              ataGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGt           >  2:5861997/1‑93 (MQ=255)
                                                                               taGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTc          <  2:4561820/93‑1 (MQ=255)
                                                                                aGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCa         <  1:6398836/93‑1 (MQ=255)
                                                                                aGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCa         >  2:1146664/1‑93 (MQ=255)
                                                                                aGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCa         <  2:1492467/93‑1 (MQ=255)
                                                                                aGTGTGGTTTGCATCTTGTTGGCGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCa         <  2:5340240/93‑1 (MQ=255)
                                                                                  tgtgGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCAtt       >  2:1271813/1‑93 (MQ=255)
                                                                                     ggTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCa    <  2:2915806/93‑1 (MQ=255)
                                                                                       tttGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGc  <  2:1317587/93‑1 (MQ=255)
                                                                                       tttGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGc  >  1:4321969/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGC  >  NC_000913/2899065‑2899244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAG  >  NC_000913/2899062‑2899248
                                                                                             |                                                                                             
CGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTG                                                                                               >  SRR4302537.962257/1‑94 (MQ=60)
 GCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGC                                                                                              <  SRR4302537.5851254/94‑1 (MQ=60)
   TGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCAT                                                                                            >  SRR4302537.4215059/1‑94 (MQ=60)
     GCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCT                                                                                          <  SRR4302537.4038056/94‑1 (MQ=60)
     GCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCT                                                                                          <  SRR4302537.1698250/94‑1 (MQ=60)
      CGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTT                                                                                         <  SRR4302537.430025/94‑1 (MQ=60)
      CGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTT                                                                                         >  SRR4302537.1648297/1‑94 (MQ=60)
       GGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTG                                                                                        >  SRR4302537.1284883/1‑94 (MQ=60)
        GTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGT                                                                                       <  SRR4302537.4651665/94‑1 (MQ=60)
         TGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTT                                                                                      >  SRR4302537.2441024/1‑94 (MQ=60)
         TGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTT                                                                                      >  SRR4302537.2494546/1‑94 (MQ=60)
          GTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTG                                                                                     >  SRR4302537.1012134/1‑94 (MQ=60)
           TTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGG                                                                                    <  SRR4302537.3731642/94‑1 (MQ=60)
           TTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGG                                                                                    >  SRR4302537.1893181/1‑94 (MQ=60)
             TACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGG                                                                                  >  SRR4302537.5866888/1‑94 (MQ=60)
             TACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGG                                                                                  >  SRR4302537.3102821/1‑94 (MQ=60)
             TACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGG                                                                                  >  SRR4302537.3827311/1‑94 (MQ=60)
              ACCATGTGGGTCTTTTCTCGCTTTGCACTGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGA                                                                                 <  SRR4302537.2714241/94‑1 (MQ=60)
              ACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGA                                                                                 >  SRR4302537.5201076/1‑94 (MQ=60)
              ACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGA                                                                                 >  SRR4302537.1266753/1‑94 (MQ=60)
               CCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGAT                                                                                >  SRR4302537.3547615/1‑94 (MQ=60)
               CCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGAT                                                                                >  SRR4302537.298392/1‑94 (MQ=60)
               CCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGAT                                                                                >  SRR4302537.5294317/1‑94 (MQ=60)
               CCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGAT                                                                                >  SRR4302537.169508/1‑94 (MQ=60)
               CCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGAT                                                                                >  SRR4302537.661198/1‑94 (MQ=60)
                CATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATT                                                                               <  SRR4302537.3581500/94‑1 (MQ=60)
                 ATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTC                                                                              <  SRR4302537.1665020/94‑1 (MQ=60)
                  TGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCT                                                                             <  SRR4302537.4945758/94‑1 (MQ=60)
                  TGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCT                                                                             <  SRR4302537.1232489/94‑1 (MQ=60)
                    TGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGT                                                                           >  SRR4302537.4485880/1‑94 (MQ=60)
                     GGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTG                                                                          <  SRR4302537.3111999/94‑1 (MQ=60)
                      GGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGC                                                                         >  SRR4302537.5418531/1‑94 (MQ=60)
                        TCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTG                                                                       <  SRR4302537.1398341/94‑1 (MQ=60)
                        TCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTG                                                                       <  SRR4302537.402409/94‑1 (MQ=60)
                         CTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTTGGTTGCATCTTGTTTGGGATTCTGTGCTGG                                                                      >  SRR4302537.2583544/1‑94 (MQ=60)
                         CTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGG                                                                      >  SRR4302537.1593601/1‑94 (MQ=60)
                          TTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGT                                                                     >  SRR4302537.1342979/1‑94 (MQ=60)
                          TTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGT                                                                     >  SRR4302537.4667321/1‑94 (MQ=60)
                           TTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTT                                                                    <  SRR4302537.4090112/94‑1 (MQ=60)
                           TTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTT                                                                    >  SRR4302537.4855803/1‑94 (MQ=60)
                             TCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTT                                                                  <  SRR4302537.4999263/94‑1 (MQ=60)
                              CTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTT                                                                 >  SRR4302537.877590/1‑94 (MQ=60)
                               TCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTT                                                                <  SRR4302537.5828127/94‑1 (MQ=60)
                                CGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTG                                                               <  SRR4302537.1229622/94‑1 (MQ=60)
                                CGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTG                                                               <  SRR4302537.2469607/94‑1 (MQ=60)
                                  CTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTT                                                             >  SRR4302537.4153764/1‑94 (MQ=60)
                                  CTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTT                                                             >  SRR4302537.5409462/1‑94 (MQ=60)
                                  CTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTT                                                             <  SRR4302537.2172661/94‑1 (MQ=60)
                                  CTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTT                                                             >  SRR4302537.4599778/1‑94 (MQ=60)
                                   TTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTA                                                            >  SRR4302537.4260111/1‑94 (MQ=60)
                                   TTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTA                                                            >  SRR4302537.3108027/1‑94 (MQ=60)
                                    TTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAG                                                           <  SRR4302537.3749594/94‑1 (MQ=60)
                                    TTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAG                                                           <  SRR4302537.4342324/94‑1 (MQ=60)
                                    TTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAG                                                           <  SRR4302537.1830879/94‑1 (MQ=60)
                                     TGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGC                                                          <  SRR4302537.773169/94‑1 (MQ=60)
                                      GCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCG                                                         <  SRR4302537.1832811/94‑1 (MQ=60)
                                        ACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGAT                                                       <  SRR4302537.5904816/94‑1 (MQ=60)
                                         CCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATA                                                      <  SRR4302537.4496492/94‑1 (MQ=60)
                                         CCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATA                                                      <  SRR4302537.5911387/94‑1 (MQ=60)
                                          CGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAA                                                     <  SRR4302537.4500528/94‑1 (MQ=60)
                                          CGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAA                                                     <  SRR4302537.2499366/94‑1 (MQ=60)
                                           GGATGACAGCACCAGCCTGAATACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAAC                                                    <  SRR4302537.3203295/94‑1 (MQ=60)
                                           GGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAAC                                                    >  SRR4302537.898409/1‑94 (MQ=60)
                                           GGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAAC                                                    <  SRR4302537.4801510/94‑1 (MQ=60)
                                            GATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACA                                                   >  SRR4302537.428991/1‑94 (MQ=60)
                                             ATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAA                                                  <  SRR4302537.1399889/94‑1 (MQ=60)
                                              TGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAAC                                                 <  SRR4302537.5380278/94‑1 (MQ=60)
                                              TGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAAC                                                 <  SRR4302537.69010/94‑1 (MQ=60)
                                              TGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAAC                                                 <  SRR4302537.4581628/94‑1 (MQ=60)
                                              TGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTATGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAAC                                                 <  SRR4302537.3300786/94‑1 (MQ=60)
                                               GACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACA                                                <  SRR4302537.3561134/94‑1 (MQ=60)
                                                ACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAA                                               >  SRR4302537.372145/1‑94 (MQ=60)
                                                ACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAA                                               >  SRR4302537.204249/1‑94 (MQ=60)
                                                 CAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAAC                                              <  SRR4302537.3066028/94‑1 (MQ=60)
                                                   GCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCT                                            <  SRR4302537.3704436/94‑1 (MQ=60)
                                                    CACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCGTCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTG                                           >  SRR4302537.3480399/1‑94 (MQ=60)
                                                    CACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTG                                           >  SRR4302537.2677871/1‑94 (MQ=60)
                                                    CACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTG                                           >  SRR4302537.4558408/1‑94 (MQ=60)
                                                     ACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGC                                          <  SRR4302537.1497531/94‑1 (MQ=60)
                                                     ACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGC                                          >  SRR4302537.5656833/1‑94 (MQ=60)
                                                      CCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCG                                         <  SRR4302537.5487343/94‑1 (MQ=60)
                                                      CCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCG                                         <  SRR4302537.4014325/94‑1 (MQ=60)
                                                      CCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCG                                         >  SRR4302537.322927/1‑94 (MQ=60)
                                                      CCAGCCTGAAAACGGTCATTATCATATATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCG                                         >  SRR4302537.4325484/1‑94 (MQ=60)
                                                        AGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCA                                       <  SRR4302537.5645241/94‑1 (MQ=60)
                                                        AGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCA                                       >  SRR4302537.1375318/1‑94 (MQ=60)
                                                         GCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAG                                      <  SRR4302537.1562516/94‑1 (MQ=60)
                                                          CCTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGT                                     >  SRR4302537.2540854/1‑94 (MQ=60)
                                                           CTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTG                                    <  SRR4302537.1420445/94‑1 (MQ=60)
                                                           CTGAAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTG                                    >  SRR4302537.6262154/1‑94 (MQ=60)
                                                            TGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGC                                   >  SRR4302537.428414/1‑94 (MQ=60)
                                                              AAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCA                                 >  SRR4302537.2960077/1‑94 (MQ=60)
                                                              AAAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCA                                 >  SRR4302537.6229192/1‑94 (MQ=60)
                                                               AAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAA                                >  SRR4302537.4271812/1‑94 (MQ=60)
                                                               AAACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAA                                >  SRR4302537.506298/1‑94 (MQ=60)
                                                                AANGNNNNNTNTCATCTATNGNNNGGTNTGCATCTTGTTGGGNATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAAT                               <  SRR4302537.5618425/94‑1 (MQ=60)
                                                                AACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAAT                               <  SRR4302537.22698/94‑1 (MQ=60)
                                                                AACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAAT                               >  SRR4302537.5567820/1‑94 (MQ=60)
                                                                 ACGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATA                              >  SRR4302537.2717461/1‑94 (MQ=60)
                                                                  CGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAA                             >  SRR4302537.1181349/1‑94 (MQ=60)
                                                                  CGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAA                             >  SRR4302537.6016456/1‑94 (MQ=60)
                                                                  CGGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAA                             <  SRR4302537.958475/94‑1 (MQ=60)
                                                                   GGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAAC                            <  SRR4302537.348385/94‑1 (MQ=60)
                                                                   GGTCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAAC                            <  SRR4302537.2448720/94‑1 (MQ=60)
                                                                     TCATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGA                          >  SRR4302537.5078691/1‑94 (MQ=60)
                                                                      CATTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAA                         >  SRR4302537.2140229/1‑94 (MQ=60)
                                                                        TTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGA                       >  SRR4302537.3352846/1‑94 (MQ=60)
                                                                        TTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGA                       <  SRR4302537.6482847/94‑1 (MQ=60)
                                                                        TTATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGA                       <  SRR4302537.5682767/94‑1 (MQ=60)
                                                                         TATCATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAA                      <  SRR4302537.5861816/94‑1 (MQ=60)
                                                                            CATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAA                   <  SRR4302537.3567593/94‑1 (MQ=60)
                                                                            CATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAA                   <  SRR4302537.1260232/94‑1 (MQ=60)
                                                                            CATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAA                   >  SRR4302537.1739632/1‑94 (MQ=60)
                                                                            CATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAA                   <  SRR4302537.3764196/94‑1 (MQ=60)
                                                                             ATCTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAAC                  <  SRR4302537.145347/94‑1 (MQ=60)
                                                                               CTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAG                >  SRR4302537.4643128/1‑94 (MQ=60)
                                                                               CTATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAG                >  SRR4302537.19792/1‑94 (MQ=60)
                                                                                TATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGT               <  SRR4302537.1321056/94‑1 (MQ=60)
                                                                                TATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGT               <  SRR4302537.534831/94‑1 (MQ=60)
                                                                                TATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGT               <  SRR4302537.2295121/94‑1 (MQ=60)
                                                                                 ATAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTC              <  SRR4302537.1737988/94‑1 (MQ=60)
                                                                                  TAGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCA             >  SRR4302537.5129557/1‑94 (MQ=60)
                                                                                   AGTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCAT            <  SRR4302537.6442403/94‑1 (MQ=60)
                                                                                    GTGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATT           <  SRR4302537.2538691/94‑1 (MQ=60)
                                                                                     TGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTC          >  SRR4302537.3647512/1‑94 (MQ=60)
                                                                                     TGTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTC          >  SRR4302537.6251005/1‑94 (MQ=60)
                                                                                      GTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCC         <  SRR4302537.6131975/94‑1 (MQ=60)
                                                                                      GTGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTAATTCA         >  SRR4302537.5624404/1‑94 (MQ=60)
                                                                                       TGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCA        <  SRR4302537.3508781/94‑1 (MQ=60)
                                                                                       TGGTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCA        <  SRR4302537.3445979/94‑1 (MQ=60)
                                                                                         GTTTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGC      >  SRR4302537.4352018/1‑94 (MQ=60)
                                                                                           TTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTT    <  SRR4302537.1714995/94‑1 (MQ=60)
                                                                                           TTGCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTT    >  SRR4302537.5279860/1‑94 (MQ=60)
                                                                                             GCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAG  <  SRR4302537.2990694/94‑1 (MQ=60)
                                                                                             GCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAG  <  SRR4302537.4854858/94‑1 (MQ=60)
                                                                                             GCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAG  >  SRR4302537.124997/1‑94 (MQ=60)
                                                                                             GCATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCAAATAACGAAGAAAAACAGTCATTCCAGCTTAG  <  SRR4302537.6068373/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAG  >  NC_000913/2899062‑2899248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑