Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,934,857 G→A E418K (GAA→AAA)  fucI → L‑fucose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,934,8570GA93.6% 733.7 / 21.4 218E418K (GAA→AAA) fucIL‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base G (7/7);  new base A (107/97);  total (114/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 235 total aligned reads displayed.

ATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCT  >  NC_000913/2934767‑2934946
                                                                                          |                                                                                         
 tcCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGaaaa                                                                                        >  2:4897806/1‑93 (MQ=255)
 tcCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGaaaa                                                                                        >  2:5092791/1‑93 (MQ=255)
 tcCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGaaaa                                                                                        >  1:2429181/1‑93 (MQ=255)
   cATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAAtc                                                                                      >  1:2868011/1‑93 (MQ=255)
    aTTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAAtct                                                                                     >  1:1165294/1‑93 (MQ=255)
     tttGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTACCCCGACGATGAAGCCACACTGGAAAAtctc                                                                                    <  1:1723902/93‑1 (MQ=255)
     tttGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAAtctc                                                                                    <  1:2028520/93‑1 (MQ=255)
      ttGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAAtctct                                                                                   <  2:2274391/93‑1 (MQ=255)
       tGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAAtctctc                                                                                  <  1:1397127/93‑1 (MQ=255)
       tGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAAtctctc                                                                                  <  1:2260329/93‑1 (MQ=255)
       tGACCAACTCCGTTTCTGCTGCGCTGGACGTTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAAtctctc                                                                                  <  2:5745328/93‑1 (MQ=255)
        gATCACCTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCa                                                                                 <  2:3333518/93‑1 (MQ=255)
          tCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGc                                                                               <  2:1067606/93‑1 (MQ=255)
          tCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGc                                                                               >  2:3694477/1‑93 (MQ=255)
            aaCTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCaa                                                                             <  2:1567697/93‑1 (MQ=255)
            aaCTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCaa                                                                             >  2:3883518/1‑93 (MQ=255)
            aaCTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCaa                                                                             <  1:3094760/93‑1 (MQ=255)
             aCTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAag                                                                            >  1:3864958/1‑93 (MQ=255)
             aCTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAag                                                                            >  2:6269016/1‑93 (MQ=255)
              cTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAaga                                                                           <  1:5811456/93‑1 (MQ=255)
              cTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAaga                                                                           <  2:5596275/93‑1 (MQ=255)
               tCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAagag                                                                          >  1:4688779/1‑93 (MQ=255)
                ccGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAgg                                                                         >  1:4023428/1‑93 (MQ=255)
                 tggTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCATCAAGAGGc                                                                        >  2:2682903/2‑93 (MQ=255)
                 cGGTTCTGCTGCGCTGGAGGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGc                                                                        <  2:5507340/93‑1 (MQ=255)
                 cGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGc                                                                        >  1:6339251/1‑93 (MQ=255)
                 cGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGc                                                                        <  1:5377401/93‑1 (MQ=255)
                 cGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGc                                                                        <  1:4300943/93‑1 (MQ=255)
                  ggTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCt                                                                       >  2:955059/1‑93 (MQ=255)
                   gTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTg                                                                      <  1:53114/93‑1 (MQ=255)
                   gTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACACGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTg                                                                      >  2:6179026/1‑93 (MQ=255)
                    ttCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGa                                                                     <  2:4484179/93‑1 (MQ=255)
                     tCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGAc                                                                    >  1:3643366/1‑93 (MQ=255)
                     tCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGAc                                                                    <  1:352170/93‑1 (MQ=255)
                     tCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGAc                                                                    >  2:6422791/1‑93 (MQ=255)
                       tgctgcGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGc                                                                  >  2:2975820/1‑93 (MQ=255)
                        tctgcGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCt                                                                 <  2:1873382/92‑1 (MQ=255)
                        gctgcGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCt                                                                 >  2:2573016/1‑93 (MQ=255)
                         ctgcGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCtt                                                                <  1:5071418/93‑1 (MQ=255)
                         ctgcGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCtt                                                                <  1:2634962/93‑1 (MQ=255)
                          tgcGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTg                                                               >  1:4337202/1‑93 (MQ=255)
                           gcgcTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGc                                                              <  1:5975481/93‑1 (MQ=255)
                             gcTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCt                                                            <  2:211410/93‑1 (MQ=255)
                              cTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTc                                                           <  1:2302345/93‑1 (MQ=255)
                               tGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTcc                                                          >  1:6454148/1‑92 (MQ=255)
                                ggACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTcgc                                                         >  1:2607969/1‑93 (MQ=255)
                                   cGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGTCTcgccgc                                                      >  2:3509146/1‑93 (MQ=255)
                                   cGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTcgccgc                                                      <  1:106025/93‑1 (MQ=255)
                                    ggTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCt                                                     <  2:2212679/93‑1 (MQ=255)
                                      ttCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTAc                                                   >  2:5907138/1‑93 (MQ=255)
                                       tCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTAcc                                                  >  2:4972764/1‑93 (MQ=255)
                                          tGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGaa                                               <  2:1288167/93‑1 (MQ=255)
                                          tGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGaa                                               >  2:419614/1‑93 (MQ=255)
                                          tGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGaa                                               <  1:5230308/93‑1 (MQ=255)
                                           gTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAAt                                              >  1:1596565/1‑93 (MQ=255)
                                           gTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAAt                                              >  1:2838482/1‑93 (MQ=255)
                                           gTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAAt                                              >  1:4000592/1‑93 (MQ=255)
                                            tAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATg                                             <  1:1636074/93‑1 (MQ=255)
                                            tAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATg                                             <  1:2734786/93‑1 (MQ=255)
                                             aaaCAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTGGCCTCGCCGCTACCGAATgg                                            <  2:6221947/93‑1 (MQ=255)
                                              aacaacGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGt                                           <  2:4780172/93‑1 (MQ=255)
                                                 aacGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGcc                                        <  2:2826545/93‑1 (MQ=255)
                                                  acGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGccc                                       <  2:4234723/93‑1 (MQ=255)
                                                   cgcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCg                                      <  1:1009367/93‑1 (MQ=255)
                                                       aCAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGa                                  >  2:4340857/1‑93 (MQ=255)
                                                         aGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATc                                >  1:5728985/1‑93 (MQ=255)
                                                          gCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATcc                               >  1:4873015/1‑93 (MQ=255)
                                                            gAAGGTACCCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCAc                             <  2:1990339/93‑1 (MQ=255)
                                                            gAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCAc                             >  2:6346942/1‑93 (MQ=255)
                                                              aGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCCCGa                           >  2:4150240/1‑93 (MQ=255)
                                                               ggTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGaa                          >  2:6041500/1‑93 (MQ=255)
                                                               ggTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGaa                          >  2:2041575/1‑93 (MQ=255)
                                                                gcaaCCCGACGATGAAGCCACGCTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAAt                         <  1:6021527/91‑1 (MQ=255)
                                                                gTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAAt                         <  1:5638369/93‑1 (MQ=255)
                                                                  accccGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATAc                       <  2:4684513/91‑1 (MQ=255)
                                                                  aaCCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATAc                       >  2:6020336/1‑93 (MQ=255)
                                                                  aaCCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATAc                       >  1:4248698/1‑93 (MQ=255)
                                                                  aaCCCGACGATGAAGCCACACTGGAAAATATCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGCTCCACGAATAc                       >  2:2877243/1‑93 (MQ=255)
                                                                   aCCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACt                      >  1:1978250/1‑93 (MQ=255)
                                                                   aCCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACt                      <  1:903494/93‑1 (MQ=255)
                                                                   aCCCGACGATGAAGCCACACTGGAAAATCTCTCAGAAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACt                      <  1:6157488/93‑1 (MQ=255)
                                                                    cccGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATGCACGAATACtt                     >  2:4374022/1‑93 (MQ=255)
                                                                    cccGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACtt                     >  1:3166169/1‑93 (MQ=255)
                                                                      cgacgaTGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTcc                   <  1:4584045/93‑1 (MQ=255)
                                                                       gacgaTGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCg                  >  1:812371/1‑93 (MQ=255)
                                                                       gacgaTGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCg                  >  1:4964485/1‑93 (MQ=255)
                                                                        acgaTGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGt                 <  1:5423686/93‑1 (MQ=255)
                                                                        acgaTGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGt                 >  2:680294/1‑93 (MQ=255)
                                                                         cgaTGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTg                <  1:5540402/93‑1 (MQ=255)
                                                                         cgaTGAAGCCACACTGAAAACTCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTg                <  1:3729577/93‑1 (MQ=255)
                                                                          gaTGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGTCCGGCGATCCACGAATACTTCCGTgg               >  2:5217557/1‑93 (MQ=255)
                                                                          gaTGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTgg               >  1:3995549/1‑93 (MQ=255)
                                                                          gaTGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTgg               >  2:3152796/1‑93 (MQ=255)
                                                                           aTGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTggc              <  2:6360710/93‑1 (MQ=255)
                                                                               aGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGgcggcg          <  1:5758522/93‑1 (MQ=255)
                                                                               aGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGgcggcg          >  2:837490/1‑93 (MQ=255)
                                                                                    cacTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTAc     <  2:1726792/93‑1 (MQ=255)
                                                                                      cTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTActc   <  2:6369242/93‑1 (MQ=255)
                                                                                      cTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTActc   >  2:399795/1‑93 (MQ=255)
                                                                                       tGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTActct  <  1:723004/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCT  >  NC_000913/2934767‑2934946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCC  >  NC_000913/2934764‑2934949
                                                                                             |                                                                                            
ATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGA                                                                                              >  SRR4302537.212921/1‑94 (MQ=60)
ATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTATAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGA                                                                                              >  SRR4302537.4218894/1‑94 (MQ=60)
 TCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAA                                                                                             <  SRR4302537.2781652/94‑1 (MQ=60)
  CATCCATTTTATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAA                                                                                            >  SRR4302537.1293852/1‑94 (MQ=60)
  CATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAA                                                                                            >  SRR4302537.2420850/1‑94 (MQ=60)
   ATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAA                                                                                           >  SRR4302537.493416/1‑94 (MQ=60)
   ATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAA                                                                                           >  SRR4302537.2446298/1‑94 (MQ=60)
    TCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAAT                                                                                          <  SRR4302537.1643759/94‑1 (MQ=60)
     CCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATC                                                                                         >  SRR4302537.2888140/1‑94 (MQ=60)
      CATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCT                                                                                        >  SRR4302537.1173395/1‑94 (MQ=60)
        TTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTACCCCGACGATGAAGCCACACTGGAAAATCTCT                                                                                      <  SRR4302537.1735948/94‑1 (MQ=60)
        TTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCT                                                                                      <  SRR4302537.2042663/94‑1 (MQ=60)
        TTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCATGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCT                                                                                      <  SRR4302537.2042662/94‑1 (MQ=60)
         TTGATCACCTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTC                                                                                     <  SRR4302537.4140244/94‑1 (MQ=60)
          TGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCA                                                                                    <  SRR4302537.2276279/94‑1 (MQ=60)
          TGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCA                                                                                    <  SRR4302537.1406866/94‑1 (MQ=60)
           GATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAG                                                                                   <  SRR4302537.5797667/94‑1 (MQ=60)
            ATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGC                                                                                  >  SRR4302537.2458668/1‑94 (MQ=60)
            ATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGC                                                                                  <  SRR4302537.1212485/94‑1 (MQ=60)
             TCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCA                                                                                 >  SRR4302537.2656843/1‑94 (MQ=60)
              CAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAA                                                                                >  SRR4302537.1731817/1‑94 (MQ=60)
              CAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAA                                                                                >  SRR4302537.326227/1‑94 (MQ=60)
               AACTCCGGTTGTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAG                                                                               <  SRR4302537.4496113/94‑1 (MQ=60)
               AACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAG                                                                               >  SRR4302537.3892011/1‑94 (MQ=60)
               AACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAG                                                                               <  SRR4302537.3116349/94‑1 (MQ=60)
                 CTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAG                                                                             <  SRR4302537.5851298/94‑1 (MQ=60)
                 CTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAG                                                                             >  SRR4302537.4721214/1‑94 (MQ=60)
                  cgCGGTTCTGCGGCGCTGGTCGGTTCCTGTAAGCAACGCGACAGCGAAGGTAACCCGACGATGAAGCAACACTGGAAAATCTCTCAGCAAGAGG                                                                            <  SRR4302537.3220202/92‑1 (MQ=60)
                  TCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGG                                                                            >  SRR4302537.4051599/1‑94 (MQ=60)
                  TCCGGTTCTGCTGCGCTGGACGGTCCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGG                                                                            <  SRR4302537.225279/94‑1 (MQ=60)
                   CCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGC                                                                           >  SRR4302537.6382570/1‑94 (MQ=60)
                    CGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCT                                                                          <  SRR4302537.3922401/94‑1 (MQ=60)
                    CGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCT                                                                          <  SRR4302537.4330833/94‑1 (MQ=60)
                    CGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCT                                                                          <  SRR4302537.5414189/94‑1 (MQ=60)
                     GGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTG                                                                         >  SRR4302537.3571606/1‑94 (MQ=60)
                     GGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTG                                                                         >  SRR4302537.3163393/1‑94 (MQ=60)
                      GTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGA                                                                        <  SRR4302537.803837/94‑1 (MQ=60)
                      GTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGA                                                                        >  SRR4302537.4554669/1‑94 (MQ=60)
                      GTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGA                                                                        <  SRR4302537.53505/94‑1 (MQ=60)
                       TTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTACCCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGAC                                                                       <  SRR4302537.3979301/94‑1 (MQ=60)
                       TTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGAC                                                                       >  SRR4302537.3669025/1‑94 (MQ=60)
                        tttcctggGCTGGACGGTTCATGAAACCAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACG                                                                      <  SRR4302537.5533916/86‑1 (MQ=60)
                        TCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACG                                                                      <  SRR4302537.354615/94‑1 (MQ=60)
                         CTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGC                                                                     >  SRR4302537.3174536/1‑94 (MQ=60)
                         CTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGC                                                                     >  SRR4302537.2976621/1‑94 (MQ=60)
                           GCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTT                                                                   <  SRR4302537.572031/94‑1 (MQ=60)
                           GCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTT                                                                   <  SRR4302537.5134925/94‑1 (MQ=60)
                            CTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTG                                                                  <  SRR4302537.5106333/94‑1 (MQ=60)
                            CTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTG                                                                  <  SRR4302537.2653400/94‑1 (MQ=60)
                            CTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTG                                                                  >  SRR4302537.4367310/1‑94 (MQ=60)
                            CTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTG                                                                  <  SRR4302537.1658269/94‑1 (MQ=60)
                             TGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGC                                                                 >  SRR4302537.1496489/1‑94 (MQ=60)
                              GCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCC                                                                >  SRR4302537.3554080/1‑94 (MQ=60)
                              GCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCC                                                                <  SRR4302537.6016505/94‑1 (MQ=60)
                               CGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCT                                                               <  SRR4302537.1799067/94‑1 (MQ=60)
                                 CTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCG                                                             <  SRR4302537.2318598/94‑1 (MQ=60)
                                 CTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCC                                                             >  SRR4302537.6497902/1‑94 (MQ=60)
                                  TGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGC                                                            >  SRR4302537.2626207/1‑94 (MQ=60)
                                    GACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCG                                                          >  SRR4302537.5744610/1‑94 (MQ=60)
                                      CGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCT                                                        <  SRR4302537.106790/94‑1 (MQ=60)
                                         cacgtgcacACAACGCGACAGCGACGCTACCCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACC                                                     <  SRR4302537.2267459/85‑1 (MQ=60)
                                           CCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGA                                                   >  SRR4302537.1017238/1‑94 (MQ=60)
                                           CCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGA                                                   >  SRR4302537.5846983/1‑94 (MQ=60)
                                             TGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAAT                                                 >  SRR4302537.1607720/1‑94 (MQ=60)
                                             TGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAAT                                                 <  SRR4302537.6230297/94‑1 (MQ=60)
                                             TGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAAT                                                 >  SRR4302537.4028601/1‑94 (MQ=60)
                                             TGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAAT                                                 >  SRR4302537.2858343/1‑94 (MQ=60)
                                             TGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAAT                                                 <  SRR4302537.5266276/94‑1 (MQ=60)
                                              GTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATG                                                >  SRR4302537.4034274/1‑94 (MQ=60)
                                               TAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGG                                               <  SRR4302537.2753869/94‑1 (MQ=60)
                                               TAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGG                                               <  SRR4302537.1647536/94‑1 (MQ=60)
                                                 AACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTG                                             >  SRR4302537.5021402/1‑94 (MQ=60)
                                                   CAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATAGTGCC                                           >  SRR4302537.6412909/1‑94 (MQ=60)
                                                    AACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCC                                          >  SRR4302537.2847297/1‑94 (MQ=60)
                                                     ACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCG                                         <  SRR4302537.5882784/94‑1 (MQ=60)
                                                      CGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGG                                        <  SRR4302537.1016328/94‑1 (MQ=60)
                                                         GACAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGA                                     >  SRR4302537.1108273/1‑94 (MQ=60)
                                                          ACAGCGAAGGTAACCCGACGATGAGGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGAT                                    <  SRR4302537.4135763/94‑1 (MQ=60)
                                                           CAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATC                                   >  SRR4302537.5768201/1‑94 (MQ=60)
                                                           CAGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATC                                   >  SRR4302537.247824/1‑94 (MQ=60)
                                                            AGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCC                                  >  SRR4302537.729729/1‑94 (MQ=60)
                                                            AGCGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCC                                  >  SRR4302537.4906647/1‑94 (MQ=60)
                                                             GCGAAGGTACCCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCA                                 <  SRR4302537.4749908/94‑1 (MQ=60)
                                                              CGAAGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCAC                                >  SRR4302537.4279067/1‑94 (MQ=60)
                                                               taaggtacgCCGACGAAGAAGCCCCACTGGAAATTCCCTCAGCAAGAGGCTGACGCTGGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACG                               <  SRR4302537.2905179/85‑1 (MQ=60)
                                                                 AGGTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAA                             >  SRR4302537.530655/1‑94 (MQ=60)
                                                                   GTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATA                           >  SRR4302537.2302936/1‑94 (MQ=60)
                                                                   GTAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATA                           <  SRR4302537.5676955/94‑1 (MQ=60)
                                                                   GCAACCCGACGATGAAGCCACGCTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATA                           <  SRR4302537.6062893/94‑1 (MQ=60)
                                                                    TAACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATAC                          >  SRR4302537.4278219/1‑94 (MQ=60)
                                                                     AACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACT                         >  SRR4302537.1991997/1‑94 (MQ=60)
                                                                     AACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACT                         >  SRR4302537.872736/1‑94 (MQ=60)
                                                                      ACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTT                        <  SRR4302537.909729/94‑1 (MQ=60)
                                                                      ACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTT                        <  SRR4302537.650264/94‑1 (MQ=60)
                                                                      ACCCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTT                        >  SRR4302537.3188409/1‑94 (MQ=60)
                                                                      ACCCGACGATGAAGCCACACTGGAAAATCTCTCAGAAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTT                        <  SRR4302537.6199865/94‑1 (MQ=60)
                                                                        CCGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCC                      <  SRR4302537.3709935/94‑1 (MQ=60)
                                                                         CGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCG                     <  SRR4302537.4615747/94‑1 (MQ=60)
                                                                         CGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCG                     >  SRR4302537.817938/1‑94 (MQ=60)
                                                                         CGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCG                     >  SRR4302537.4998705/1‑94 (MQ=60)
                                                                         CGACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTCCCCGGCGATCCACGAATACTTCCG                     <  SRR4302537.5990248/94‑1 (MQ=60)
                                                                          GACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGT                    <  SRR4302537.2860124/94‑1 (MQ=60)
                                                                           ACGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTG                   <  SRR4302537.5460655/94‑1 (MQ=60)
                                                                           ACGATGAAGCCACACTGAAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTG                   <  SRR4302537.28451/94‑1 (MQ=60)
                                                                            CGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGG                  <  SRR4302537.5578231/94‑1 (MQ=60)
                                                                            CGATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGG                  >  SRR4302537.4023516/1‑94 (MQ=60)
                                                                            CGATGAAGCCACACTGAAAACTCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGG                  <  SRR4302537.3755777/94‑1 (MQ=60)
                                                                             GATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGC                 >  SRR4302537.5650370/1‑94 (MQ=60)
                                                                             GATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGC                 <  SRR4302537.605787/94‑1 (MQ=60)
                                                                              ATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCG                <  SRR4302537.6112608/94‑1 (MQ=60)
                                                                              ATGAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCG                <  SRR4302537.6223415/94‑1 (MQ=60)
                                                                                GAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGC              >  SRR4302537.4026579/1‑94 (MQ=60)
                                                                                GAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGC              <  SRR4302537.5567638/94‑1 (MQ=60)
                                                                                GAAGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGC              <  SRR4302537.4675522/94‑1 (MQ=60)
                                                                                  AGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGG            >  SRR4302537.4347179/1‑94 (MQ=60)
                                                                                  AGCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGG            <  SRR4302537.5797967/94‑1 (MQ=60)
                                                                                   GCCACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGT           >  SRR4302537.2640107/1‑94 (MQ=60)
                                                                                     CACACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTA         <  SRR4302537.5460009/94‑1 (MQ=60)
                                                                                        ACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTC      >  SRR4302537.226688/1‑94 (MQ=60)
                                                                                        ACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTC      >  SRR4302537.5293519/1‑94 (MQ=60)
                                                                                        ACTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTC      >  SRR4302537.538097/1‑94 (MQ=60)
                                                                                         CTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCT     >  SRR4302537.4982997/1‑94 (MQ=60)
                                                                                         CTGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGCTACTCT     >  SRR4302537.3777620/1‑94 (MQ=60)
                                                                                          TGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTT    >  SRR4302537.1773262/1‑94 (MQ=60)
                                                                                          TGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTT    <  SRR4302537.727939/94‑1 (MQ=60)
                                                                                          TGGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTT    >  SRR4302537.5058372/1‑94 (MQ=60)
                                                                                           GGAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTC   >  SRR4302537.1144939/1‑94 (MQ=60)
                                                                                           GGAAAACCTCTCAGCAAGAGGCTGCCGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTC   <  SRR4302537.2507553/94‑1 (MQ=60)
                                                                                            GAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCC  <  SRR4302537.5127856/94‑1 (MQ=60)
                                                                                            GAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCC  <  SRR4302537.4061851/94‑1 (MQ=60)
                                                                                            GAAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCAGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCC  <  SRR4302537.4767726/94‑1 (MQ=60)
                                                                                             |                                                                                            
ATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCC  >  NC_000913/2934764‑2934949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑