Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,972,555	A→G	S494S (AGT→AGC)	aas ←	2‑acyl‑glycerophospho‑ethanolamine acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,972,555	0	A	G	99.6%	814.8 / ‑4.6	239	S494S (AGT→AGC)	aas	2‑acyl‑glycerophospho‑ethanolamine acyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (122/115); total (122/117)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

Only 100 of 241 total aligned reads displayed.

TACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAA  >  NC_000913/2972465‑2972644

 aCGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAACTTTATCCTGCCGAAGGTGTTTGGTGCtt                                                                                        >  2:1414294/1‑93 (MQ=255)

 aCGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCtt                                                                                        >  2:1720897/1‑93 (MQ=255)

   cttGACAGAAACGACAGCCGCGCATTCGGTCATGCCGTAGCCTTCAAGGATTCGCAGGCTAAATTTATCCTGCAAAAGCTGTTTGGTGCTTTc                                                                                      <  1:6068741/92‑1 (MQ=255)

      gATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTg                                                                                   <  1:4353816/93‑1 (MQ=255)

      gATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTg                                                                                   >  1:4834369/1‑93 (MQ=255)

        tAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTa                                                                                 >  2:5583268/1‑93 (MQ=255)

        tAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTa                                                                                 <  1:23742/93‑1 (MQ=255)

           aaaCGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAAtt                                                                              <  2:805706/93‑1 (MQ=255)

           aaaCGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAAtt                                                                              <  1:3993797/93‑1 (MQ=255)

           aaaCGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAAtt                                                                              <  2:3705496/93‑1 (MQ=255)

              cGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAAttttt                                                                           <  1:4085282/93‑1 (MQ=255)

               gACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTc                                                                          <  2:5744677/93‑1 (MQ=255)

               gACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTc                                                                          <  2:5993343/93‑1 (MQ=255)

               gACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTc                                                                          <  1:4272518/93‑1 (MQ=255)

                aCAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCt                                                                         <  1:450707/93‑1 (MQ=255)

                 cAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTg                                                                        <  1:2907158/93‑1 (MQ=255)

                 cAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTg                                                                        <  2:3984505/93‑1 (MQ=255)

                   ggCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTgcg                                                                      >  1:2259500/1‑93 (MQ=255)

                    gcgcgcATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTgcgc                                                                     <  1:5307467/93‑1 (MQ=255)

                    gcgcgcATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTgcgc                                                                     <  2:6464328/93‑1 (MQ=255)

                     cgcgcCTTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGcc                                                                    <  1:286870/93‑1 (MQ=255)

                     cgcgcATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGcc                                                                    <  1:2843181/93‑1 (MQ=255)

                      gcgcATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCt                                                                   >  2:2865493/1‑93 (MQ=255)

                          aTTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGcca                                                               >  1:1996158/1‑93 (MQ=255)

                          aTTCGGTCACGCCGTAGACTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGcca                                                               >  2:4441628/1‑93 (MQ=255)

                           ttCGGTCACGCCGTAGCCTTGAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGccac                                                              <  1:3656258/93‑1 (MQ=255)

                           ttCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGccac                                                              <  1:761811/93‑1 (MQ=255)

                           ttCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGccac                                                              >  1:4786891/1‑93 (MQ=255)

                           ttCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGccac                                                              >  2:2944271/1‑93 (MQ=255)

                           ttCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGccac                                                              >  1:3410690/1‑93 (MQ=255)

                            tCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGccacc                                                             >  2:4514400/1‑93 (MQ=255)

                            tCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGccacc                                                             <  2:3558895/93‑1 (MQ=255)

                              ggTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCcaccac                                                           >  2:302026/1‑93 (MQ=255)

                                tCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACAt                                                         <  2:5066719/93‑1 (MQ=255)

                                    gCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCg                                                     >  2:5401111/1‑93 (MQ=255)

                                     ccGTAGCCTTCAAGGATGCGCAGGCCAAATTTATTCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGt                                                    >  1:6138226/1‑93 (MQ=255)

                                     ccGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGt                                                    <  1:5918407/93‑1 (MQ=255)

                                      cGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTa                                                   >  1:1709060/1‑93 (MQ=255)

                                       gTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAg                                                  >  1:884351/1‑93 (MQ=255)

                                       gTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAg                                                  >  1:4619081/1‑93 (MQ=255)

                                        tAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGa                                                 >  1:985880/1‑93 (MQ=255)

                                         aGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGAc                                                <  2:2310732/93‑1 (MQ=255)

                                          gCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTTATTTTTCTGCGCCTGCCACCACATAGCGTAGACg                                               >  1:314926/1‑93 (MQ=255)

                                           ctttCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGa                                              <  1:1049186/91‑1 (MQ=255)

                                           ccTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGa                                              >  2:4844730/1‑93 (MQ=255)

                                            cTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGAt                                             <  2:4230833/93‑1 (MQ=255)

                                             ttCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATa                                            <  1:2235656/93‑1 (MQ=255)

                                                 aGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAg                                        <  2:2478998/93‑1 (MQ=255)

                                                 aGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAg                                        >  1:5702996/1‑93 (MQ=255)

                                                 aGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAg                                        <  1:546354/93‑1 (MQ=255)

                                                   gATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTc                                      >  1:2693968/1‑93 (MQ=255)

                                                     tGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCAt                                    >  1:3849342/1‑93 (MQ=255)

                                                      gcgcAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATa                                   <  1:3035787/93‑1 (MQ=255)

                                                       cgcAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATAc                                  <  1:1571540/93‑1 (MQ=255)

                                                       cgcAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATAc                                  <  2:1583324/93‑1 (MQ=255)

                                                        gcAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGTTAGAAGTCATACg                                 >  1:3689119/1‑93 (MQ=255)

                                                        gcAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACg                                 >  1:3514370/1‑93 (MQ=255)

                                                         cAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACgg                                <  1:4730002/93‑1 (MQ=255)

                                                         cAGGCAAAATTTAGCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACgg                                <  2:2048944/93‑1 (MQ=255)

                                                          aGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACggg                               >  2:5923701/1‑93 (MQ=255)

                                                          aGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACggg                               >  2:5706630/1‑93 (MQ=255)

                                                          aGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACggg                               >  1:3366476/1‑93 (MQ=255)

                                                          aGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACggg                               <  1:1931912/93‑1 (MQ=255)

                                                           ggCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGt                              <  2:1121745/93‑1 (MQ=255)

                                                            gCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGtt                             <  2:3444258/93‑1 (MQ=255)

                                                              cAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTgg                           >  2:2562022/1‑93 (MQ=255)

                                                               aaaTTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGc                          >  1:2449801/1‑93 (MQ=255)

                                                               aaaTTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGc                          >  2:2735566/1‑93 (MQ=255)

                                                               aaaTTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGc                          >  2:2976392/1‑93 (MQ=255)

                                                                aaTTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCg                         >  1:4977522/1‑93 (MQ=255)

                                                                 aTTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGa                        >  2:3252789/1‑93 (MQ=255)

                                                                    tATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGaaag                     >  2:3289202/1‑92 (MQ=255)

                                                                      tCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAAcgc                   >  2:1450982/1‑93 (MQ=255)

                                                                      tCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAAcgc                   <  1:1211094/93‑1 (MQ=255)

                                                                       ccTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAAcgcg                  >  2:4725299/1‑93 (MQ=255)

                                                                       ccTGCAAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAAcgcg                  <  1:5538585/93‑1 (MQ=255)

                                                                        cTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAAcgcgc                 <  2:61736/93‑1 (MQ=255)

                                                                        cTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAAcgcgc                 <  2:4305833/93‑1 (MQ=255)

                                                                        cTGCCAAAGCTGTGTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAAcgcgc                 <  1:5777718/93‑1 (MQ=255)

                                                                         tGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAAcgcgcg                >  1:3142481/1‑93 (MQ=255)

                                                                          gCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGt               >  1:5013639/1‑93 (MQ=255)

                                                                           ccAAGGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTa              <  2:4062962/93‑1 (MQ=255)

                                                                           ccAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCGACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTa              >  1:4560881/1‑93 (MQ=255)

                                                                           ccAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTa              <  1:5641635/93‑1 (MQ=255)

                                                                             aaaGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGt            >  2:3777319/1‑93 (MQ=255)

                                                                             aaaGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGt            >  1:1332531/1‑93 (MQ=255)

                                                                              aaGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTg           >  2:6136818/1‑93 (MQ=255)

                                                                              aaGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTg           >  1:6315319/1‑93 (MQ=255)

                                                                                gCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGAc         >  1:4217173/1‑93 (MQ=255)

                                                                                gCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGAc         <  2:1031215/93‑1 (MQ=255)

                                                                                 cTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGAcc        <  2:18818/93‑1 (MQ=255)

                                                                                 cTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGAcc        <  2:1851883/93‑1 (MQ=255)

                                                                                 cTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGAcc        >  1:1016812/1‑93 (MQ=255)

                                                                                   gTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGa      >  2:1605209/1‑93 (MQ=255)

                                                                                   gTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGa      >  2:589428/1‑93 (MQ=255)

                                                                                   gTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGa      <  2:1217418/93‑1 (MQ=255)

                                                                                    tttGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAg     >  2:1153668/1‑93 (MQ=255)

                                                                                    tttGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAg     <  1:386735/93‑1 (MQ=255)

                                                                                      tGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGa   <  2:525503/93‑1 (MQ=255)

                                                                                      tGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGa   <  1:2602732/93‑1 (MQ=255)

TACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAA  >  NC_000913/2972465‑2972644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTC  >  NC_000913/2972462‑2972648

TGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCTTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTG                                                                                               <  SRR4302537.3536066/94‑1 (MQ=60)

CGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTG                                                                                               <  SRR4302537.4865095/94‑1 (MQ=60)

  GTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCT                                                                                             <  SRR4302537.4657478/94‑1 (MQ=60)

     CGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTC                                                                                          <  SRR4302537.6322934/94‑1 (MQ=60)

      GTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCT                                                                                         <  SRR4302537.1973631/94‑1 (MQ=60)

      GTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGACGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCT                                                                                         <  SRR4302537.3488364/94‑1 (MQ=60)

        TGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTG                                                                                       >  SRR4302537.4867749/1‑94 (MQ=60)

         GATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGT                                                                                      >  SRR4302537.5054130/1‑94 (MQ=60)

         GATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGT                                                                                      <  SRR4302537.4383963/94‑1 (MQ=60)

           TAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAA                                                                                    <  SRR4302537.23926/94‑1 (MQ=60)

             GAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATT                                                                                  >  SRR4302537.3392474/1‑94 (MQ=60)

             GAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATT                                                                                  <  SRR4302537.5718684/94‑1 (MQ=60)

              AAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTT                                                                                 <  SRR4302537.4021759/94‑1 (MQ=60)

              AAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTT                                                                                 <  SRR4302537.3916841/94‑1 (MQ=60)

               AACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTT                                                                                >  SRR4302537.5538688/1‑94 (MQ=60)

               AACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTT                                                                                <  SRR4302537.4856473/94‑1 (MQ=60)

                 CGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTC                                                                              <  SRR4302537.608213/94‑1 (MQ=60)

                 CGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTC                                                                              <  SRR4302537.4113807/94‑1 (MQ=60)

                  GACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCT                                                                             <  SRR4302537.1792870/94‑1 (MQ=60)

                  GACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCT                                                                             <  SRR4302537.4302207/94‑1 (MQ=60)

                   ACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTG                                                                            <  SRR4302537.453825/94‑1 (MQ=60)

                    CAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGC                                                                           <  SRR4302537.2927583/94‑1 (MQ=60)

                    CAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGC                                                                           <  SRR4302537.2470465/94‑1 (MQ=60)

                     AGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCG                                                                          >  SRR4302537.2433990/1‑94 (MQ=60)

                     AGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCG                                                                          >  SRR4302537.2275444/1‑94 (MQ=60)

                      GGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGC                                                                         >  SRR4302537.2375002/1‑94 (MQ=60)

                       GCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCC                                                                        <  SRR4302537.5343935/94‑1 (MQ=60)

                        CGCGCCTTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCT                                                                       <  SRR4302537.288868/94‑1 (MQ=60)

                        CGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCT                                                                       <  SRR4302537.2863080/94‑1 (MQ=60)

                          CGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGC                                                                     >  SRR4302537.1717444/1‑94 (MQ=60)

                            CATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCA                                                                   >  SRR4302537.2622581/1‑94 (MQ=60)

                            CATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCA                                                                   >  SRR4302537.2010035/1‑94 (MQ=60)

                            CATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCA                                                                   <  SRR4302537.2904220/94‑1 (MQ=60)

                             ATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCAC                                                                  >  SRR4302537.3434846/1‑94 (MQ=60)

                             ATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCAC                                                                  >  SRR4302537.4819928/1‑94 (MQ=60)

                              TTCGGTCACGCCGTAGCCTTGAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACC                                                                 <  SRR4302537.3681989/94‑1 (MQ=60)

                              TTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACC                                                                 <  SRR4302537.767016/94‑1 (MQ=60)

                                 GGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACA                                                              <  SRR4302537.5552330/94‑1 (MQ=60)

                                 GGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACA                                                              >  SRR4302537.3126472/1‑94 (MQ=60)

                                  GTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACAT                                                             >  SRR4302537.203050/1‑94 (MQ=60)

                                     ACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGC                                                          >  SRR4302537.227083/1‑94 (MQ=60)

                                      CGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCG                                                         >  SRR4302537.1040756/1‑94 (MQ=60)

                                      CGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCG                                                         <  SRR4302537.770966/94‑1 (MQ=60)

                                       GCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATTCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGT                                                        >  SRR4302537.6180431/1‑94 (MQ=60)

                                       GCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGT                                                        >  SRR4302537.4365583/1‑94 (MQ=60)

                                       GCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGT                                                        >  SRR4302537.1181696/1‑94 (MQ=60)

                                        CCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTA                                                       <  SRR4302537.5959020/94‑1 (MQ=60)

                                        CCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTA                                                       >  SRR4302537.1721001/1‑94 (MQ=60)

                                        CCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTA                                                       >  SRR4302537.5490982/1‑94 (MQ=60)

                                         CGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAG                                                      >  SRR4302537.4651068/1‑94 (MQ=60)

                                         CGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAG                                                      >  SRR4302537.890437/1‑94 (MQ=60)

                                          GTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGA                                                     >  SRR4302537.992687/1‑94 (MQ=60)

                                          GTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGA                                                     >  SRR4302537.5053074/1‑94 (MQ=60)

                                            AGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTTATTTTTCTGCGCCTGCCACCACATAGCGTAGACG                                                   >  SRR4302537.317107/1‑94 (MQ=60)

                                            AGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACG                                                   <  SRR4302537.4686485/94‑1 (MQ=60)

                                              CTTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGAT                                                 <  SRR4302537.1056420/94‑1 (MQ=60)

                                                TTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAG                                               <  SRR4302537.1591255/94‑1 (MQ=60)

                                                TTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAG                                               <  SRR4302537.2251423/94‑1 (MQ=60)

                                                CTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAG                                               <  SRR4302537.2292726/94‑1 (MQ=60)

                                                 TCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGA                                              <  SRR4302537.1298951/94‑1 (MQ=60)

                                                 TCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGA                                              <  SRR4302537.5615836/94‑1 (MQ=60)

                                                 TCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGA                                              <  SRR4302537.205878/94‑1 (MQ=60)

                                                   AAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAG                                            >  SRR4302537.5742023/1‑94 (MQ=60)

                                                   AAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAG                                            <  SRR4302537.3882865/94‑1 (MQ=60)

                                                   AAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGCAGACGATAGAAG                                            >  SRR4302537.4845372/1‑94 (MQ=60)

                                                   AAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCCCCTGCCACCACATAGCGTAGACGATAGAAG                                            >  SRR4302537.5802494/1‑94 (MQ=60)

                                                    AGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGT                                           >  SRR4302537.4656122/1‑94 (MQ=60)

                                                    AGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGT                                           >  SRR4302537.4257677/1‑94 (MQ=60)

                                                    AGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGT                                           <  SRR4302537.550117/94‑1 (MQ=60)

                                                    AGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGT                                           >  SRR4302537.5042071/1‑94 (MQ=60)

                                                     GGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTC                                          >  SRR4302537.2712792/1‑94 (MQ=60)

                                                       ATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCAT                                        >  SRR4302537.3876299/1‑94 (MQ=60)

                                                         GCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATAC                                      <  SRR4302537.3056949/94‑1 (MQ=60)

                                                          CGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGTTAGAAGTCATACG                                     >  SRR4302537.3715065/1‑94 (MQ=60)

                                                          CGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACG                                     <  SRR4302537.1582507/94‑1 (MQ=60)

                                                          CGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACG                                     >  SRR4302537.3539233/1‑94 (MQ=60)

                                                           GCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGG                                    >  SRR4302537.4138242/1‑94 (MQ=60)

                                                            CAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGG                                   >  SRR4302537.3390321/1‑94 (MQ=60)

                                                            CAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGG                                   <  SRR4302537.4762687/94‑1 (MQ=60)

                                                             AGGCTAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGT                                  <  SRR4302537.288123/94‑1 (MQ=60)

                                                             AGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGT                                  >  SRR4302537.5006920/1‑94 (MQ=60)

                                                             AGGCCAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGT                                  <  SRR4302537.1945351/94‑1 (MQ=60)

                                                              GGCCAAATTTATCCTGCCAAAGATGTTTGGTGCTTTCTTGTAAGTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTT                                 >  SRR4302537.3497083/1‑94 (MQ=60)

                                                                 CAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGC                              >  SRR4302537.2467034/1‑94 (MQ=60)

                                                                 CAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGC                              >  SRR4302537.3661941/1‑94 (MQ=60)

                                                                 CAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGC                              >  SRR4302537.2645853/1‑94 (MQ=60)

                                                                 CAAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGCAGACGATAGAAGTCATACGGGTTGGC                              >  SRR4302537.4595900/1‑94 (MQ=60)

                                                                  AAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCG                             >  SRR4302537.426124/1‑94 (MQ=60)

                                                                  AAATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCG                             >  SRR4302537.5011828/1‑94 (MQ=60)

                                                                   AATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACTGGTTGGCGA                            >  SRR4302537.616510/1‑94 (MQ=60)

                                                                   AATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGA                            >  SRR4302537.1409603/1‑94 (MQ=60)

                                                                    ATTTATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAA                           >  SRR4302537.6391373/1‑94 (MQ=60)

                                                                       TATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAGACG                        >  SRR4302537.4393455/1‑94 (MQ=60)

                                                                        ATCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGC                       >  SRR4302537.4572053/1‑94 (MQ=60)

                                                                         TCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCG                      >  SRR4302537.2878833/1‑94 (MQ=60)

                                                                         TCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCG                      >  SRR4302537.274487/1‑94 (MQ=60)

                                                                         TCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCG                      <  SRR4302537.1219519/94‑1 (MQ=60)

                                                                         TCCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCG                      >  SRR4302537.989557/1‑94 (MQ=60)

                                                                          CCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGC                     >  SRR4302537.3931511/1‑94 (MQ=60)

                                                                          CCTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCATAGACGATAGAAGTCATACGGGTTGGCGAAACGCGC                     <  SRR4302537.3762238/94‑1 (MQ=60)

                                                                          CCTGCAAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGC                     <  SRR4302537.5576392/94‑1 (MQ=60)

                                                                           CTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCG                    <  SRR4302537.6057973/94‑1 (MQ=60)

                                                                           CTGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCG                    >  SRR4302537.3164536/1‑94 (MQ=60)

                                                                           CTGCCAAAGCTGTGTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCG                    <  SRR4302537.5817323/94‑1 (MQ=60)

                                                                            TGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGT                   >  SRR4302537.5048177/1‑94 (MQ=60)

                                                                            TGCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGT                   <  SRR4302537.5710332/94‑1 (MQ=60)

                                                                             GCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCGACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTA                  >  SRR4302537.4592452/1‑94 (MQ=60)

                                                                             GCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTA                  >  SRR4302537.3117105/1‑94 (MQ=60)

                                                                             GCCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTA                  >  SRR4302537.368200/1‑94 (MQ=60)

                                                                              CCAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAG                 <  SRR4302537.5680244/94‑1 (MQ=60)

                                                                               CAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGT                <  SRR4302537.3235600/94‑1 (MQ=60)

                                                                               CAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGT                >  SRR4302537.1341816/1‑94 (MQ=60)

                                                                               CAAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGT                <  SRR4302537.3164647/94‑1 (MQ=60)

                                                                                AAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTG               >  SRR4302537.6358542/1‑94 (MQ=60)

                                                                                AAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTG               >  SRR4302537.964392/1‑94 (MQ=60)

                                                                                AAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTG               <  SRR4302537.479343/94‑1 (MQ=60)

                                                                                AAAGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTG               >  SRR4302537.45258/1‑94 (MQ=60)

                                                                                  AGCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGAC             >  SRR4302537.4246568/1‑94 (MQ=60)

                                                                                   GCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATGGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACC            <  SRR4302537.1280626/94‑1 (MQ=60)

                                                                                   GCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACC            >  SRR4302537.3248710/1‑94 (MQ=60)

                                                                                   GCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACC            >  SRR4302537.1023813/1‑94 (MQ=60)

                                                                                   GCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACC            <  SRR4302537.4102073/94‑1 (MQ=60)

                                                                                   GCTGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTAGCGAAACGCGCGTAGTGACC            >  SRR4302537.6243492/1‑94 (MQ=60)

                                                                                     TGTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGA          >  SRR4302537.3793985/1‑94 (MQ=60)

                                                                                      GTTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAG         >  SRR4302537.5863045/1‑94 (MQ=60)

                                                                                       TTTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGG        <  SRR4302537.389408/94‑1 (MQ=60)

                                                                                        TTGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGA       <  SRR4302537.2966139/94‑1 (MQ=60)

                                                                                         TGGTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAA      <  SRR4302537.2620929/94‑1 (MQ=60)

                                                                                           GTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAG    >  SRR4302537.382356/1‑94 (MQ=60)

                                                                                           GTGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAG    <  SRR4302537.3419011/94‑1 (MQ=60)

                                                                                            TGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGT   >  SRR4302537.1230493/1‑94 (MQ=60)

                                                                                            TGCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGT   >  SRR4302537.2944920/1‑94 (MQ=60)

                                                                                             TCTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTC  <  SRR4302537.2474788/94‑1 (MQ=60)

CGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTC  >  NC_000913/2972462‑2972648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`