Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,223	A→G	T61A (ACC→GCC)	lysR →	LysR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,223	0	A	G	100.0%	884.2 / NA	249	T61A (ACC→GCC)	lysR	LysR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (115/134); total (115/134)

Only 100 of 253 total aligned reads displayed.

TCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG  >  NC_000913/2977133‑2977312

  ccaGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGt                                                                                       <  2:3358051/92‑1 (MQ=255)

  acaGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGt                                                                                       <  2:1700104/93‑1 (MQ=255)

  acaGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGt                                                                                       >  2:2406596/1‑93 (MQ=255)

  acaGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGt                                                                                       >  1:6152478/1‑93 (MQ=255)

  acaGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGt                                                                                       <  1:1807456/93‑1 (MQ=255)

     gCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCa                                                                                    >  2:5804246/1‑93 (MQ=255)

     gCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCa                                                                                    <  1:4927810/93‑1 (MQ=255)

      ccAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCaa                                                                                   <  1:519705/93‑1 (MQ=255)

       cAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAg                                                                                  <  2:1754564/93‑1 (MQ=255)

       cAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAg                                                                                  >  1:3792203/1‑93 (MQ=255)

       cAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAg                                                                                  <  1:5622142/93‑1 (MQ=255)

        aaCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAgg                                                                                 >  1:5900112/1‑93 (MQ=255)

         aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAGATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGa                                                                                <  1:5104043/93‑1 (MQ=255)

         aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGa                                                                                <  2:1224967/93‑1 (MQ=255)

         aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATACTGCCGTGCAAggc                                                                                >  1:4568582/1‑92 (MQ=255)

          ccGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGAc                                                                               <  1:2648778/93‑1 (MQ=255)

          ccGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGAc                                                                               <  1:2028056/93‑1 (MQ=255)

           cGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACt                                                                              <  1:5390757/93‑1 (MQ=255)

            gTCAGCCGCGCACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTg                                                                             >  2:965290/1‑93 (MQ=255)

            gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTg                                                                             <  1:1015962/93‑1 (MQ=255)

             tCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGc                                                                            <  2:1990757/93‑1 (MQ=255)

             tCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGc                                                                            <  2:2526025/93‑1 (MQ=255)

             tCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGc                                                                            >  1:2438706/1‑93 (MQ=255)

               aGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGt                                                                          <  1:4889971/93‑1 (MQ=255)

                gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTc                                                                         <  2:2201798/93‑1 (MQ=255)

                 ccGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCt                                                                        <  2:1900804/93‑1 (MQ=255)

                 ccGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCt                                                                        <  1:3127308/93‑1 (MQ=255)

                  cgcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTg                                                                       <  1:4384227/93‑1 (MQ=255)

                    cgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGtt                                                                     <  1:2528840/93‑1 (MQ=255)

                     gAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGttt                                                                    <  1:887934/93‑1 (MQ=255)

                     gAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGttt                                                                    <  1:1307787/93‑1 (MQ=255)

                     gAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGttt                                                                    >  2:687947/1‑93 (MQ=255)

                      aaCTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTg                                                                   >  1:1726351/1‑93 (MQ=255)

                      aaCTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTg                                                                   >  1:2585907/1‑93 (MQ=255)

                        cTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTgaa                                                                 <  2:3172419/93‑1 (MQ=255)

                        cTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTgaa                                                                 <  2:4538552/93‑1 (MQ=255)

                         ttGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTgaag                                                                >  2:6210365/1‑93 (MQ=255)

                         ttGCGCGCTTGGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTgaag                                                                <  1:2822267/93‑1 (MQ=255)

                             gcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGt                                                            >  1:1388385/1‑93 (MQ=255)

                             gcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGt                                                            >  1:4254946/1‑93 (MQ=255)

                              cgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTg                                                           <  1:3888667/93‑1 (MQ=255)

                               gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGc                                                          >  1:4634481/1‑93 (MQ=255)

                               gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGc                                                          >  1:3443120/1‑93 (MQ=255)

                               gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGc                                                          <  2:5052602/93‑1 (MQ=255)

                               gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGc                                                          <  2:3265663/93‑1 (MQ=255)

                                 tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                        <  2:2045829/93‑1 (MQ=255)

                                 tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                        >  2:4872254/1‑93 (MQ=255)

                                 tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                        >  2:3898331/1‑93 (MQ=255)

                                 tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                        <  2:4223998/93‑1 (MQ=255)

                                 tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                        >  2:433133/1‑93 (MQ=255)

                                 tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                        <  2:760155/93‑1 (MQ=255)

                                   tGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACg                                                      >  1:5792098/1‑93 (MQ=255)

                                        aGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCt                                                 <  1:5303742/93‑1 (MQ=255)

                                         ggTGATCGGGCTGAAATTGTTTGAGCTCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTg                                                <  1:5930591/93‑1 (MQ=255)

                                         ggTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTg                                                <  1:2124933/93‑1 (MQ=255)

                                          gTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTATTTGAAGAAGTGCAACGATCCTgg                                               <  2:5732791/93‑1 (MQ=255)

                                           tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGt                                              <  1:1557350/93‑1 (MQ=255)

                                            gATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTa                                             <  1:116729/93‑1 (MQ=255)

                                              tCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACg                                           >  2:1805035/1‑93 (MQ=255)

                                              tCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACg                                           <  1:3262607/93‑1 (MQ=255)

                                                  gCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACt                                       <  1:1919408/93‑1 (MQ=255)

                                                    tGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTgg                                     <  2:4997831/93‑1 (MQ=255)

                                                     gAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGa                                    <  2:5294627/93‑1 (MQ=255)

                                                      aaaTTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGCAGTGCAACGATCCTGGTACGGACTGGAt                                   >  1:6068653/1‑93 (MQ=255)

                                                      aaaTTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGAt                                   >  1:96049/1‑93 (MQ=255)

                                                      aaaTTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGAt                                   <  2:2641017/93‑1 (MQ=255)

                                                         ttGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGc                                <  1:3296071/93‑1 (MQ=255)

                                                          tgtgtgAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCa                               <  1:648495/93‑1 (MQ=255)

                                                          tGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCa                               <  2:2174639/93‑1 (MQ=255)

                                                          tGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCa                               <  2:2745023/93‑1 (MQ=255)

                                                           gTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                              >  2:3915559/1‑93 (MQ=255)

                                                           gTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                              <  1:5735872/93‑1 (MQ=255)

                                                            tttGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAtt                             >  2:1410372/1‑93 (MQ=255)

                                                            tttGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAtt                             <  1:5559057/93‑1 (MQ=255)

                                                            tttGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAtt                             >  1:4085990/1‑93 (MQ=255)

                                                            tttGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAtt                             <  1:5508576/93‑1 (MQ=255)

                                                               gAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTc                          <  1:4399886/93‑1 (MQ=255)

                                                               gAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTc                          <  2:4813384/93‑1 (MQ=255)

                                                               gAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTc                          >  1:276124/1‑93 (MQ=255)

                                                                aGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCa                         <  2:2755033/93‑1 (MQ=255)

                                                                      tACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccg                   >  2:3503652/1‑93 (MQ=255)

                                                                        cGTGGGTGATTACATCCTGCCGTGCAAGGACTGCGTAGGTGTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCa                 <  2:359102/93‑1 (MQ=255)

                                                                        cGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCa                 >  1:3930396/1‑93 (MQ=255)

                                                                         gTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAg                >  1:3679490/1‑93 (MQ=255)

                                                                          tGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGa               >  2:3630173/1‑93 (MQ=255)

                                                                          tGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGa               >  1:629754/1‑93 (MQ=255)

                                                                           gggCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGaa              <  2:6016747/93‑1 (MQ=255)

                                                                             gCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAg            >  2:3839538/1‑93 (MQ=255)

                                                                              cGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGt           <  2:1578834/93‑1 (MQ=255)

                                                                               gATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTc          <  2:2845307/93‑1 (MQ=255)

                                                                                aTTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCt         >  2:6116890/1‑93 (MQ=255)

                                                                                  tACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTgc       >  2:4274863/1‑93 (MQ=255)

                                                                                  tACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTgc       >  2:4102391/1‑93 (MQ=255)

                                                                                  tACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTgc       <  2:6352961/93‑1 (MQ=255)

                                                                                   aCATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTgcg      <  2:4604066/93‑1 (MQ=255)

                                                                                      tCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGa   >  2:5390745/1‑93 (MQ=255)

                                                                                      tCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGa   <  2:6271173/93‑1 (MQ=255)

                                                                                      tCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGa   <  1:6357546/93‑1 (MQ=255)

                                                                                       ccTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAg  >  2:6420908/1‑93 (MQ=255)

                                                                                       ccTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAg  <  1:2960613/93‑1 (MQ=255)

TCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG  >  NC_000913/2977133‑2977312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTC  >  NC_000913/2977130‑2977316

ACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTG                                                                                               >  SRR4302537.5674248/1‑94 (MQ=60)

ACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTG                                                                                               >  SRR4302537.5951867/1‑94 (MQ=60)

 CCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGC                                                                                              >  SRR4302537.2241157/1‑94 (MQ=60)

  CTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCC                                                                                             >  SRR4302537.3861593/1‑94 (MQ=60)

  CTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCC                                                                                             <  SRR4302537.5158212/94‑1 (MQ=60)

    CACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGT                                                                                           >  SRR4302537.3588740/1‑94 (MQ=60)

    CACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGT                                                                                           >  SRR4302537.1940650/1‑94 (MQ=60)

    CACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGT                                                                                           >  SRR4302537.6194801/1‑94 (MQ=60)

     ACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTG                                                                                          <  SRR4302537.1820066/94‑1 (MQ=60)

       AGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCA                                                                                        >  SRR4302537.6081954/1‑94 (MQ=60)

        GCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAA                                                                                       <  SRR4302537.6173758/94‑1 (MQ=60)

        GCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAA                                                                                       >  SRR4302537.2741210/1‑94 (MQ=60)

        GCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAA                                                                                       <  SRR4302537.4961781/94‑1 (MQ=60)

        GCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAA                                                                                       <  SRR4302537.4634258/94‑1 (MQ=60)

         CCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAG                                                                                      <  SRR4302537.523281/94‑1 (MQ=60)

         CCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAG                                                                                      <  SRR4302537.4192483/94‑1 (MQ=60)

         CCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAG                                                                                      >  SRR4302537.3818816/1‑94 (MQ=60)

          CAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGG                                                                                     <  SRR4302537.5660579/94‑1 (MQ=60)

          CAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGG                                                                                     >  SRR4302537.5940596/1‑94 (MQ=60)

           AACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGA                                                                                    >  SRR4302537.2515785/1‑94 (MQ=60)

           AACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATACTGCCGTGCAAGGC                                                                                    >  SRR4302537.4600200/1‑94 (MQ=60)

            ACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAGATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGAC                                                                                   <  SRR4302537.5139217/94‑1 (MQ=60)

            ACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGAC                                                                                   <  SRR4302537.5405370/94‑1 (MQ=60)

             CCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACT                                                                                  <  SRR4302537.2042195/94‑1 (MQ=60)

             CCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACT                                                                                  <  SRR4302537.2835798/94‑1 (MQ=60)

             CCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACT                                                                                  <  SRR4302537.2667304/94‑1 (MQ=60)

             CCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACT                                                                                  <  SRR4302537.1719541/94‑1 (MQ=60)

              CGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTG                                                                                 <  SRR4302537.5427584/94‑1 (MQ=60)

               GTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGC                                                                                <  SRR4302537.1022960/94‑1 (MQ=60)

               GTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGC                                                                                >  SRR4302537.2455884/1‑94 (MQ=60)

                TCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCG                                                                               <  SRR4302537.3593203/94‑1 (MQ=60)

                  AGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTC                                                                             <  SRR4302537.4923740/94‑1 (MQ=60)

                  AGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTC                                                                             <  SRR4302537.5193543/94‑1 (MQ=60)

                   GCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCT                                                                            <  SRR4302537.6439687/94‑1 (MQ=60)

                    CCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTG                                                                           >  SRR4302537.5715607/1‑94 (MQ=60)

                    CCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTG                                                                           <  SRR4302537.3149228/94‑1 (MQ=60)

                     CGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGT                                                                          <  SRR4302537.4414482/94‑1 (MQ=60)

                      GCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTT                                                                         >  SRR4302537.3797725/1‑94 (MQ=60)

                       CGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTT                                                                        <  SRR4302537.2546584/94‑1 (MQ=60)

                       CGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTT                                                                        >  SRR4302537.3414749/1‑94 (MQ=60)

                       CGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTT                                                                        >  SRR4302537.1447833/1‑94 (MQ=60)

                        GAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTG                                                                       >  SRR4302537.2604000/1‑94 (MQ=60)

                        GAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTG                                                                       <  SRR4302537.4036371/94‑1 (MQ=60)

                        GAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTG                                                                       >  SRR4302537.587288/1‑94 (MQ=60)

                        GAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTG                                                                       <  SRR4302537.1316892/94‑1 (MQ=60)

                        GAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTG                                                                       >  SRR4302537.1738413/1‑94 (MQ=60)

                        GAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTG                                                                       <  SRR4302537.894040/94‑1 (MQ=60)

                        GAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTG                                                                       <  SRR4302537.5414599/94‑1 (MQ=60)

                         AACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGA                                                                      >  SRR4302537.3273990/1‑94 (MQ=60)

                          ACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAA                                                                     >  SRR4302537.4065898/1‑94 (MQ=60)

                          ACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAA                                                                     <  SRR4302537.5969270/94‑1 (MQ=60)

                           CTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAG                                                                    <  SRR4302537.6204224/94‑1 (MQ=60)

                            TTGCGCGCTTGGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGA                                                                   <  SRR4302537.2841995/94‑1 (MQ=60)

                             TGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAA                                                                  >  SRR4302537.3890078/1‑94 (MQ=60)

                             TGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAA                                                                  <  SRR4302537.1776651/94‑1 (MQ=60)

                               CGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGT                                                                >  SRR4302537.1398063/1‑94 (MQ=60)

                               CGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGT                                                                >  SRR4302537.4284522/1‑94 (MQ=60)

                               CGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGG                                                                >  SRR4302537.4830019/1‑94 (MQ=60)

                                 CGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGC                                                              >  SRR4302537.3467480/1‑94 (MQ=60)

                                 CGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGC                                                              <  SRR4302537.3915868/94‑1 (MQ=60)

                                 CGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGC                                                              >  SRR4302537.4666538/1‑94 (MQ=60)

                                  GCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCA                                                             <  SRR4302537.2277889/94‑1 (MQ=60)

                                     TTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACG                                                          >  SRR4302537.5831810/1‑94 (MQ=60)

                                        AGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATC                                                       >  SRR4302537.3071073/1‑94 (MQ=60)

                                          AAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                     <  SRR4302537.1385381/94‑1 (MQ=60)

                                           AGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTG                                                    <  SRR4302537.5621116/94‑1 (MQ=60)

                                           AGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTG                                                    <  SRR4302537.5340198/94‑1 (MQ=60)

                                            GGTGATCGGGCTGAAATTGTTTGAGCTCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGG                                                   <  SRR4302537.5971281/94‑1 (MQ=60)

                                            GGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGG                                                   >  SRR4302537.687804/1‑94 (MQ=60)

                                            GGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGG                                                   <  SRR4302537.1139812/94‑1 (MQ=60)

                                            GGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGG                                                   <  SRR4302537.2139882/94‑1 (MQ=60)

                                              TGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTA                                                 <  SRR4302537.5727122/94‑1 (MQ=60)

                                              TGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTA                                                 <  SRR4302537.1568221/94‑1 (MQ=60)

                                               GATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAC                                                >  SRR4302537.2972473/1‑94 (MQ=60)

                                               GATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAC                                                <  SRR4302537.117572/94‑1 (MQ=60)

                                                ATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACG                                               <  SRR4302537.956043/94‑1 (MQ=60)

                                                ATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACG                                               <  SRR4302537.3292895/94‑1 (MQ=60)

                                                 TCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGG                                              <  SRR4302537.3285620/94‑1 (MQ=60)

                                                  CGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGA                                             >  SRR4302537.4416107/1‑94 (MQ=60)

                                                  CGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGA                                             <  SRR4302537.5996105/94‑1 (MQ=60)

                                                     GCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTG                                          >  SRR4302537.392252/1‑94 (MQ=60)

                                                     GCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTG                                          <  SRR4302537.1932735/94‑1 (MQ=60)

                                                       TGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACTATCCTGGTACGGACTGGA                                        >  SRR4302537.4696999/1‑94 (MQ=60)

                                                        GAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGCAGTGCAACGATCCTGGTACGGACTGGAT                                       >  SRR4302537.6110348/1‑94 (MQ=60)

                                                        GAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGAT                                       >  SRR4302537.96750/1‑94 (MQ=60)

                                                           ATTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGC                                    >  SRR4302537.5013264/1‑94 (MQ=60)

                                                            TTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCT                                   >  SRR4302537.2573010/1‑94 (MQ=60)

                                                            TTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCA                                   <  SRR4302537.3319369/94‑1 (MQ=60)

                                                            TTGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCA                                   >  SRR4302537.1177520/1‑94 (MQ=60)

                                                             TGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAT                                  >  SRR4302537.2013651/1‑94 (MQ=60)

                                                             TGTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAT                                  <  SRR4302537.4470491/94‑1 (MQ=60)

                                                             TGTGTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAT                                  <  SRR4302537.652916/94‑1 (MQ=60)

                                                              GTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATT                                 >  SRR4302537.4114521/1‑94 (MQ=60)

                                                              GTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATT                                 >  SRR4302537.5932347/1‑94 (MQ=60)

                                                              GTTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATT                                 <  SRR4302537.5775147/94‑1 (MQ=60)

                                                               TTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTG                                <  SRR4302537.5597044/94‑1 (MQ=60)

                                                               TTTGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTG                                <  SRR4302537.5546198/94‑1 (MQ=60)

                                                                 TGAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTC                              >  SRR4302537.278058/1‑94 (MQ=60)

                                                                  GAGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCA                             <  SRR4302537.4430248/94‑1 (MQ=60)

                                                                   AGCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAG                            <  SRR4302537.2560504/94‑1 (MQ=60)

                                                                    GCGCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGC                           >  SRR4302537.2660935/1‑94 (MQ=60)

                                                                      GCGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGC                         >  SRR4302537.4960524/1‑94 (MQ=60)

                                                                       CGTACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCC                        <  SRR4302537.329950/94‑1 (MQ=60)

                                                                         TACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGC                      <  SRR4302537.6430499/94‑1 (MQ=60)

                                                                         TACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGC                      >  SRR4302537.2051589/1‑94 (MQ=60)

                                                                          ACGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCA                     >  SRR4302537.3957858/1‑94 (MQ=60)

                                                                           CGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAG                    >  SRR4302537.3705378/1‑94 (MQ=60)

                                                                           CGTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAG                    <  SRR4302537.4503/94‑1 (MQ=60)

                                                                            GTGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGA                   >  SRR4302537.634037/1‑94 (MQ=60)

                                                                             TGGGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAA                  >  SRR4302537.1848048/1‑94 (MQ=60)

                                                                               GGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAG                <  SRR4302537.5543417/94‑1 (MQ=60)

                                                                               GGCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAG                <  SRR4302537.1784370/94‑1 (MQ=60)

                                                                                GCGATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGT               <  SRR4302537.5811208/94‑1 (MQ=60)

                                                                                  GATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCT             >  SRR4302537.5175017/1‑94 (MQ=60)

                                                                                  GATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCAGCAGAAAGTAT             >  SRR4302537.6396265/1‑94 (MQ=60)

                                                                                   ATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTG            <  SRR4302537.1499261/94‑1 (MQ=60)

                                                                                   ATTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTG            >  SRR4302537.5113729/1‑94 (MQ=60)

                                                                                    TTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGC           <  SRR4302537.5572352/94‑1 (MQ=60)

                                                                                    TTACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGC           >  SRR4302537.118010/1‑94 (MQ=60)

                                                                                     TACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCG          <  SRR4302537.2668809/94‑1 (MQ=60)

                                                                                      ACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGC         <  SRR4302537.4325747/94‑1 (MQ=60)

                                                                                      ACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGC         >  SRR4302537.5573830/1‑94 (MQ=60)

                                                                                      ACATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGC         >  SRR4302537.284242/1‑94 (MQ=60)

                                                                                       CATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCG        <  SRR4302537.6319696/94‑1 (MQ=60)

                                                                                        ATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGA       >  SRR4302537.2330925/1‑94 (MQ=60)

                                                                                        ATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGA       >  SRR4302537.5322294/1‑94 (MQ=60)

                                                                                        ATCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGA       >  SRR4302537.4314051/1‑94 (MQ=60)

                                                                                         TCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG      <  SRR4302537.6400934/94‑1 (MQ=60)

                                                                                         TCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG      <  SRR4302537.927751/94‑1 (MQ=60)

                                                                                         TCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG      >  SRR4302537.3145949/1‑94 (MQ=60)

                                                                                          CCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGT     <  SRR4302537.2981401/94‑1 (MQ=60)

                                                                                           CTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTT    <  SRR4302537.2793032/94‑1 (MQ=60)

                                                                                            TGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTT   >  SRR4302537.3092227/1‑94 (MQ=60)

                                                                                             GCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTC  <  SRR4302537.5718728/94‑1 (MQ=60)

ACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTC  >  NC_000913/2977130‑2977316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`