Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,011,187 T→C E358E (GAA→GAG)  b2875 ← putative synthases

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,011,1870TC99.7% 1018.4 / ‑5.0 286E358E (GAA→GAG) b2875putative synthases
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (142/143);  total (143/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

Only 100 of 289 total aligned reads displayed.

CCCAAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGTTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAG  >  NC_000913/3011097‑3011276
                                                                                          |                                                                                         
 ccAAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCtca                                                                                        <  1:3448860/93‑1 (MQ=255)
 ccAAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCtca                                                                                        >  1:2461102/1‑93 (MQ=255)
  cAAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCtcat                                                                                       >  2:3472480/1‑93 (MQ=255)
   caGCGCCAATGTTTATGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCtcatc                                                                                      <  1:2020842/92‑1 (MQ=255)
   aaGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCtcatc                                                                                      <  2:3159341/93‑1 (MQ=255)
    aGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCtcatca                                                                                     >  1:3187473/1‑93 (MQ=255)
    aGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCtcatca                                                                                     >  1:5352699/1‑93 (MQ=255)
    aGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCtcatca                                                                                     >  2:4149435/1‑93 (MQ=255)
      cgcgATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCATAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAg                                                                                   <  2:4004495/93‑1 (MQ=255)
       gcgATTGTTGCTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGc                                                                                  <  2:1692700/93‑1 (MQ=255)
       gcgATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGc                                                                                  <  2:6153967/93‑1 (MQ=255)
        cgATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCa                                                                                 >  1:4777845/1‑93 (MQ=255)
        cgATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCa                                                                                 <  2:2223505/93‑1 (MQ=255)
         gATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCaa                                                                                <  2:4233547/93‑1 (MQ=255)
          aTTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAAt                                                                               >  1:2546926/1‑93 (MQ=255)
          aTTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAAt                                                                               >  2:5922024/1‑93 (MQ=255)
            tGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGa                                                                             >  2:2309868/1‑93 (MQ=255)
             gTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGaa                                                                            <  1:2340097/93‑1 (MQ=255)
             gTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGaa                                                                            <  1:736279/93‑1 (MQ=255)
              ttACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAAc                                                                           >  2:692280/1‑93 (MQ=255)
               tACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCTGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACa                                                                          <  2:1299510/93‑1 (MQ=255)
               tACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACa                                                                          <  1:2792741/93‑1 (MQ=255)
                aCTGGTGCGATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAg                                                                         <  1:1483888/93‑1 (MQ=255)
                 cTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAgg                                                                        <  2:1702519/93‑1 (MQ=255)
                     tGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCggg                                                                    >  1:353884/1‑93 (MQ=255)
                     tGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCggg                                                                    >  2:1834069/1‑93 (MQ=255)
                       ccATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGAt                                                                  >  1:5205089/1‑93 (MQ=255)
                        cATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAACGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGAtg                                                                 >  2:1155830/1‑92 (MQ=255)
                        cATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATc                                                                 >  2:5640020/1‑93 (MQ=255)
                         atatCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCt                                                                >  1:4159742/1‑93 (MQ=255)
                         atatCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCt                                                                >  1:2649272/1‑93 (MQ=255)
                          tatCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTa                                                               >  2:1616999/1‑93 (MQ=255)
                           atCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTAc                                                              >  2:3409406/1‑93 (MQ=255)
                            tCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTAcc                                                             <  2:468295/93‑1 (MQ=255)
                              tGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCAt                                                           >  1:3638977/1‑93 (MQ=255)
                              tGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCAt                                                           >  2:5013542/1‑93 (MQ=255)
                               gCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATc                                                          >  2:870572/1‑93 (MQ=255)
                                cccGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATca                                                         >  1:2166705/1‑93 (MQ=255)
                                cccGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATca                                                         <  2:1288144/93‑1 (MQ=255)
                                  cgcgTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATcaca                                                       <  1:5404553/93‑1 (MQ=255)
                                    cgTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAgg                                                     <  2:2563668/93‑1 (MQ=255)
                                    cgTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAgg                                                     <  1:1872680/93‑1 (MQ=255)
                                     gTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGga                                                    <  2:628371/93‑1 (MQ=255)
                                     gTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGga                                                    >  2:3590769/1‑93 (MQ=255)
                                     gTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGga                                                    >  1:1179975/1‑93 (MQ=255)
                                      ttCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGgaa                                                   <  2:6035624/93‑1 (MQ=255)
                                      ttCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGgaa                                                   <  1:4484690/93‑1 (MQ=255)
                                      ttCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGgaa                                                   <  2:5870245/93‑1 (MQ=255)
                                          cAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGgaatgaa                                               >  2:5356242/1‑93 (MQ=255)
                                          cAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGgaatgaa                                               >  2:4845698/1‑93 (MQ=255)
                                          cAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGgaatgaa                                               >  1:437238/1‑93 (MQ=255)
                                          cAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGgaatgaa                                               >  1:6223654/1‑93 (MQ=255)
                                           aaaTTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGgaatgaat                                              <  1:803805/93‑1 (MQ=255)
                                           aaaTTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGgaatgaat                                              >  1:5990201/1‑93 (MQ=255)
                                            aaTTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATc                                             <  1:4972130/93‑1 (MQ=255)
                                            aaTTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATc                                             >  2:140458/1‑93 (MQ=255)
                                            aaTTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATc                                             >  1:6153152/1‑93 (MQ=255)
                                             aTTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATcc                                            >  1:1675236/1‑93 (MQ=255)
                                              ttCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATGcg                                           >  1:996238/1‑93 (MQ=255)
                                              ttCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCcg                                           >  2:2077432/1‑93 (MQ=255)
                                               tCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCcgc                                          <  1:3872735/93‑1 (MQ=255)
                                               tCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCcgc                                          >  2:6355626/1‑93 (MQ=255)
                                                 tGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGt                                        >  1:3986954/1‑93 (MQ=255)
                                                  gTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGtt                                       >  2:6346531/1‑93 (MQ=255)
                                                   tttCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTc                                      <  2:3926097/93‑1 (MQ=255)
                                                    ttCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCg                                     >  1:6395808/1‑93 (MQ=255)
                                                     tCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCgg                                    >  2:2809596/1‑93 (MQ=255)
                                                        ccAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTaa                                 <  1:4885610/93‑1 (MQ=255)
                                                        ccAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTaa                                 <  2:3367000/93‑1 (MQ=255)
                                                        ccAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTaa                                 >  1:3091571/1‑93 (MQ=255)
                                                         cAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAg                                >  2:3045108/1‑93 (MQ=255)
                                                          aGAATAGCGTCCCCCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGt                               <  2:3242837/93‑1 (MQ=255)
                                                          aGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTGAGt                               >  2:2496330/1‑93 (MQ=255)
                                                          aGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGt                               >  1:861998/1‑93 (MQ=255)
                                                          aGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGt                               <  1:1451534/93‑1 (MQ=255)
                                                           gAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGtt                              >  1:3021583/1‑93 (MQ=255)
                                                            aaTAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGttt                             >  2:5124946/1‑93 (MQ=255)
                                                             aTAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTc                            >  2:4342231/1‑93 (MQ=255)
                                                              tAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCa                           >  1:4069786/1‑93 (MQ=255)
                                                               aGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCAt                          >  1:581541/1‑93 (MQ=255)
                                                               aGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCAt                          >  1:508274/1‑93 (MQ=255)
                                                                   tCCCCCGCGCAAAGCGTTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTGCCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCt                      <  2:147174/93‑1 (MQ=255)
                                                                   tCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCt                      >  1:4337325/1‑93 (MQ=255)
                                                                    ccaccaCGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCtt                     >  2:2433993/1‑93 (MQ=255)
                                                                     caccacGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTc                    <  2:2672045/93‑1 (MQ=255)
                                                                     caccacGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTc                    >  1:132900/1‑93 (MQ=255)
                                                                     caccacGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTc                    >  1:3277393/1‑93 (MQ=255)
                                                                      cccacGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTcc                   <  1:2857775/92‑1 (MQ=255)
                                                                      accacGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTcc                   >  1:1524384/1‑93 (MQ=255)
                                                                           gCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCaga              <  1:1123862/93‑1 (MQ=255)
                                                                           gCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCaga              <  2:3865519/93‑1 (MQ=255)
                                                                             aaaGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGTATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGc            <  1:4432188/93‑1 (MQ=255)
                                                                              aaGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCt           <  2:1334635/93‑1 (MQ=255)
                                                                               aGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTg          >  2:5525479/1‑93 (MQ=255)
                                                                                gCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATCAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTgg         <  2:5265524/93‑1 (MQ=255)
                                                                                   gTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTg      >  2:3801938/1‑93 (MQ=255)
                                                                                      tCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCa   >  2:2331367/1‑93 (MQ=255)
                                                                                      tCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCa   <  1:5675432/93‑1 (MQ=255)
                                                                                      tCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGGTCGGTAAGTTTGATGGCTTCGGCAGAGCTGGTGGccg   >  2:4538696/1‑92 (MQ=255)
                                                                                       cAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAg  >  2:3230469/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCCAAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGTTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAG  >  NC_000913/3011097‑3011276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCCCAAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGTTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAGGCGA  >  NC_000913/3011095‑3011280
                                                                                            |                                                                                             
GGCCCAAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCT                                                                                              <  SRR4302537.622317/94‑1 (MQ=60)
  CCCAAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCA                                                                                            >  SRR4302537.2478408/1‑94 (MQ=60)
  CCCAAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCA                                                                                            >  SRR4302537.4096831/1‑94 (MQ=60)
   CCAAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCAT                                                                                           <  SRR4302537.3473256/94‑1 (MQ=60)
    CAAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATA                                                                                          >  SRR4302537.5258946/1‑94 (MQ=60)
     cagcgccaatgtttaTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCA                                                                                         <  SRR4302537.2034939/79‑1 (MQ=60)
     AAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCA                                                                                         >  SRR4302537.4269172/1‑94 (MQ=60)
     AAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCA                                                                                         >  SRR4302537.3209895/1‑94 (MQ=60)
     AAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCA                                                                                         >  SRR4302537.4436166/1‑94 (MQ=60)
     AAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCA                                                                                         >  SRR4302537.5389361/1‑94 (MQ=60)
       GCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAG                                                                                       <  SRR4302537.4499412/94‑1 (MQ=60)
       GCGCGATTGTTACTGGTGCCATATCGGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAG                                                                                       <  SRR4302537.504344/94‑1 (MQ=60)
         GCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCA                                                                                     >  SRR4302537.4810831/1‑94 (MQ=60)
          CGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAA                                                                                    <  SRR4302537.147489/94‑1 (MQ=60)
          CGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGAAA                                                                                    >  SRR4302537.4469049/1‑94 (MQ=60)
           GATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAAT                                                                                   >  SRR4302537.2564769/1‑94 (MQ=60)
           GATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAAT                                                                                   >  SRR4302537.1921142/1‑94 (MQ=60)
           GATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAAT                                                                                   >  SRR4302537.5058585/1‑94 (MQ=60)
            ATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATG                                                                                  >  SRR4302537.1503825/1‑94 (MQ=60)
             TTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGA                                                                                 >  SRR4302537.3986527/1‑94 (MQ=60)
              TGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAA                                                                                >  SRR4302537.3880957/1‑94 (MQ=60)
              TGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAA                                                                                <  SRR4302537.721601/94‑1 (MQ=60)
               GTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAAC                                                                               <  SRR4302537.741301/94‑1 (MQ=60)
               GTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAAC                                                                               <  SRR4302537.3109426/94‑1 (MQ=60)
               GTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAAC                                                                               <  SRR4302537.2356587/94‑1 (MQ=60)
                 TACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAG                                                                             <  SRR4302537.2812258/94‑1 (MQ=60)
                 TACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAG                                                                             <  SRR4302537.6251605/94‑1 (MQ=60)
                  ACTGGTGCGATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGG                                                                            <  SRR4302537.1494213/94‑1 (MQ=60)
                  ACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGG                                                                            <  SRR4302537.6386537/94‑1 (MQ=60)
                  ACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGG                                                                            >  SRR4302537.767655/1‑94 (MQ=60)
                   CTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGC                                                                           <  SRR4302537.1332886/94‑1 (MQ=60)
                   CTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGC                                                                           <  SRR4302537.4449443/94‑1 (MQ=60)
                    TGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCG                                                                          <  SRR4302537.2445448/94‑1 (MQ=60)
                      GTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGG                                                                        >  SRR4302537.356345/1‑94 (MQ=60)
                      GTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGG                                                                        >  SRR4302537.2540258/1‑94 (MQ=60)
                      GTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGG                                                                        <  SRR4302537.174664/94‑1 (MQ=60)
                      GTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGG                                                                        <  SRR4302537.203497/94‑1 (MQ=60)
                        GCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGAT                                                                      <  SRR4302537.4883907/94‑1 (MQ=60)
                        GCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGAT                                                                      >  SRR4302537.5240894/1‑94 (MQ=60)
                        GCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATAAAGCAATGAACAGGCGGGAT                                                                      <  SRR4302537.850658/94‑1 (MQ=60)
                         CCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATC                                                                     >  SRR4302537.2699627/1‑94 (MQ=60)
                          CATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCT                                                                    >  SRR4302537.2667800/1‑94 (MQ=60)
                          CATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCT                                                                    >  SRR4302537.4188792/1‑94 (MQ=60)
                             ATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACC                                                                 >  SRR4302537.4266362/1‑94 (MQ=60)
                              TCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCA                                                                <  SRR4302537.155885/94‑1 (MQ=60)
                               CTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCAT                                                               >  SRR4302537.3664599/1‑94 (MQ=60)
                                TGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATC                                                              <  SRR4302537.624742/94‑1 (MQ=60)
                                TGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATC                                                              <  SRR4302537.3143266/94‑1 (MQ=60)
                                 GCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCA                                                             >  SRR4302537.1360856/1‑94 (MQ=60)
                                 GCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCA                                                             >  SRR4302537.2181967/1‑94 (MQ=60)
                                  CCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCAC                                                            <  SRR4302537.1183570/94‑1 (MQ=60)
                                  CCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCAC                                                            >  SRR4302537.3333779/1‑94 (MQ=60)
                                    CGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAG                                                          <  SRR4302537.5441425/94‑1 (MQ=60)
                                      CGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGA                                                        <  SRR4302537.1885711/94‑1 (MQ=60)
                                      CGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGA                                                        >  SRR4302537.1188186/1‑94 (MQ=60)
                                      CGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGA                                                        >  SRR4302537.2839833/1‑94 (MQ=60)
                                       GTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAA                                                       >  SRR4302537.2756424/1‑94 (MQ=60)
                                       GTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAA                                                       >  SRR4302537.5695186/1‑94 (MQ=60)
                                       GTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAA                                                       >  SRR4302537.6455749/1‑94 (MQ=60)
                                       GTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAA                                                       <  SRR4302537.5719641/94‑1 (MQ=60)
                                        TTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAAT                                                      <  SRR4302537.4515714/94‑1 (MQ=60)
                                        TTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAAT                                                      <  SRR4302537.5734928/94‑1 (MQ=60)
                                         TCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATG                                                     >  SRR4302537.4399793/1‑94 (MQ=60)
                                         TCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATG                                                     >  SRR4302537.2542398/1‑94 (MQ=60)
                                         TCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATG                                                     >  SRR4302537.4825520/1‑94 (MQ=60)
                                          CCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGA                                                    <  SRR4302537.3285056/94‑1 (MQ=60)
                                           CCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAA                                                   >  SRR4302537.6266489/1‑94 (MQ=60)
                                           CCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAA                                                   >  SRR4302537.440286/1‑94 (MQ=60)
                                            CAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGCAT                                                  >  SRR4302537.536710/1‑94 (MQ=60)
                                            CAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAAT                                                  >  SRR4302537.6031339/1‑94 (MQ=60)
                                            CAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAAT                                                  <  SRR4302537.4927702/94‑1 (MQ=60)
                                            CAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAAT                                                  >  SRR4302537.1308457/1‑94 (MQ=60)
                                             AAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATC                                                 <  SRR4302537.616150/94‑1 (MQ=60)
                                             AAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATC                                                 >  SRR4302537.6195480/1‑94 (MQ=60)
                                             AAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATC                                                 <  SRR4302537.809323/94‑1 (MQ=60)
                                              AATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCC                                                <  SRR4302537.5006402/94‑1 (MQ=60)
                                              AATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCC                                                >  SRR4302537.4020199/1‑94 (MQ=60)
                                              AATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCC                                                >  SRR4302537.1686964/1‑94 (MQ=60)
                                              AATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCC                                                <  SRR4302537.5657720/94‑1 (MQ=60)
                                              AATTCTGTGTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATTAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCC                                                <  SRR4302537.5006401/94‑1 (MQ=60)
                                               ATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATGCG                                               >  SRR4302537.1003119/1‑94 (MQ=60)
                                                TTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGC                                              >  SRR4302537.6163776/1‑94 (MQ=60)
                                                 TCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCG                                             <  SRR4302537.3899832/94‑1 (MQ=60)
                                                  CTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGT                                            >  SRR4302537.4014872/1‑94 (MQ=60)
                                                   TGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGTTCATCAAGCAATGAACAGGCGGGAGCTACCATCACAGGAATGAATTCGCGTT                                           >  SRR4302537.178368/1‑94 (MQ=60)
                                                   TGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTT                                           >  SRR4302537.3079351/1‑94 (MQ=60)
                                                   TGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTT                                           >  SRR4302537.4650142/1‑94 (MQ=60)
                                                   TGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTT                                           >  SRR4302537.6404070/1‑94 (MQ=60)
                                                     TTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCG                                         >  SRR4302537.6439363/1‑94 (MQ=60)
                                                      TTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGG                                        <  SRR4302537.1502394/94‑1 (MQ=60)
                                                       TCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGT                                       <  SRR4302537.5158581/94‑1 (MQ=60)
                                                         GCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAA                                     >  SRR4302537.3113136/1‑94 (MQ=60)
                                                          CCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGGTCTACCATCACAGGAATGAATCCGCGTTCGGTAAG                                    >  SRR4302537.4106635/1‑94 (MQ=60)
                                                          CCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAG                                    <  SRR4302537.2867338/94‑1 (MQ=60)
                                                          CCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAG                                    <  SRR4302537.4919350/94‑1 (MQ=60)
                                                           CAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGT                                   <  SRR4302537.168680/94‑1 (MQ=60)
                                                           CAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGT                                   >  SRR4302537.867909/1‑94 (MQ=60)
                                                           CAGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGT                                   <  SRR4302537.418402/94‑1 (MQ=60)
                                                            AGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTT                                  >  SRR4302537.5134971/1‑94 (MQ=60)
                                                            AGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTT                                  <  SRR4302537.1461649/94‑1 (MQ=60)
                                                            AGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTT                                  >  SRR4302537.3042647/1‑94 (MQ=60)
                                                            AGAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTT                                  >  SRR4302537.3538978/1‑94 (MQ=60)
                                                             GAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTT                                 <  SRR4302537.2160696/94‑1 (MQ=60)
                                                             GAATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTT                                 <  SRR4302537.3950153/94‑1 (MQ=60)
                                                              AATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTC                                >  SRR4302537.138832/1‑94 (MQ=60)
                                                               ATAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCA                               >  SRR4302537.4098211/1‑94 (MQ=60)
                                                                TAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCAT                              >  SRR4302537.511763/1‑94 (MQ=60)
                                                                TAGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCAT                              >  SRR4302537.585526/1‑94 (MQ=60)
                                                                 AGCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATC                             <  SRR4302537.2126374/94‑1 (MQ=60)
                                                                  GCGTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCG                            <  SRR4302537.5932769/94‑1 (MQ=60)
                                                                    GTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCT                          >  SRR4302537.3365101/1‑94 (MQ=60)
                                                                    GTCCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCT                          >  SRR4302537.4367433/1‑94 (MQ=60)
                                                                    GTCCACCACGCAAAGCGGTTTCAGCTCATCAAGAAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCT                          <  SRR4302537.2608510/94‑1 (MQ=60)
                                                                      CCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTC                        >  SRR4302537.3300524/1‑94 (MQ=60)
                                                                      CCACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTC                        >  SRR4302537.133841/1‑94 (MQ=60)
                                                                       CACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCC                       >  SRR4302537.4682484/1‑94 (MQ=60)
                                                                       CACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCC                       <  SRR4302537.3652149/94‑1 (MQ=60)
                                                                       CACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCC                       <  SRR4302537.3024780/94‑1 (MQ=60)
                                                                       CACCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCC                       >  SRR4302537.1535032/1‑94 (MQ=60)
                                                                        CCCACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCG                      <  SRR4302537.2877812/94‑1 (MQ=60)
                                                                          CACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCA                    >  SRR4302537.929103/1‑94 (MQ=60)
                                                                          CACGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCA                    <  SRR4302537.5939944/94‑1 (MQ=60)
                                                                            CGCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGA                  >  SRR4302537.3875895/1‑94 (MQ=60)
                                                                             GCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTGCCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAG                 >  SRR4302537.3486898/1‑94 (MQ=60)
                                                                             GCAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAG                 <  SRR4302537.1131656/94‑1 (MQ=60)
                                                                              CAAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGC                >  SRR4302537.2242641/1‑94 (MQ=60)
                                                                               AAAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGTATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCT               <  SRR4302537.4462793/94‑1 (MQ=60)
                                                                                AAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTG              <  SRR4302537.3619937/94‑1 (MQ=60)
                                                                                AAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTG              <  SRR4302537.2570511/94‑1 (MQ=60)
                                                                                AAGCGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTG              >  SRR4302537.2404676/1‑94 (MQ=60)
                                                                                   CGGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGATTCATCGCTTCCGCAGAGCTGGTT           >  SRR4302537.3162612/1‑94 (MQ=60)
                                                                                    GGTTTCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTG          <  SRR4302537.1476936/94‑1 (MQ=60)
                                                                                        TCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAG      <  SRR4302537.5714264/94‑1 (MQ=60)
                                                                                        TCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAG      <  SRR4302537.5422988/94‑1 (MQ=60)
                                                                                        TCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAG      <  SRR4302537.757870/94‑1 (MQ=60)
                                                                                        TCAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAG      <  SRR4302537.300976/94‑1 (MQ=60)
                                                                                         CAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAGG     >  SRR4302537.5200378/1‑94 (MQ=60)
                                                                                         CAGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAGG     <  SRR4302537.990660/94‑1 (MQ=60)
                                                                                          AGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAGGC    <  SRR4302537.6114381/94‑1 (MQ=60)
                                                                                          AGCTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAGGC    <  SRR4302537.5918502/94‑1 (MQ=60)
                                                                                            CTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAGGCGA  >  SRR4302537.3351026/1‑94 (MQ=60)
                                                                                            |                                                                                             
GGCCCAAGCGCGATTGTTACTGGTGCCATATCTGCCCGCGTTCCCAAATTCTGTTTCGCCAGAATAGCGTCCACCACGCAAAGCGGTTTCAGTTCATCAAGCAATGAACAGGCGGGATCTACCATCACAGGAATGAATCCGCGTTCGGTAAGTTTCATCGCTTCCGCAGAGCTGGTTGCCAGGCGA  >  NC_000913/3011095‑3011280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑